CN105076136B - New opplication of the epiphysin in plant resistant damage or crop failure caused by waterlogging stress is improved - Google Patents

Abstract

The present invention relates to new opplication of the epiphysin in plant resistant damage or crop failure caused by waterlogging stress is improved.Product with epiphysin as active ingredient is imposed on the plant coerced by damage or crop failure caused by waterlogging, the ability that plant removes ROS can be significantly increased, mitigate the oxidative damage that damage or crop failure caused by waterlogging Stress on Plant is caused, hence it is evident that improve the ability of plant resistant damage or crop failure caused by waterlogging stress, so as to reduce yield and quality caused by damage or crop failure caused by waterlogging decline.

Description

New Application of Melatonin in Improving Plant Resistance to Waterlogging Stress

technical field

The invention relates to a new application of melatonin in improving plant resistance to waterlogging stress.

Background technique

Waterlogging is the main natural disaster. The precipitation in summer and autumn is concentrated and abundant. If the drainage of farmland is not timely, it is easy to cause crop waterlogging. When crops suffer from waterlogging, the level of reactive oxygen species in the crops will increase, breaking the original metabolic balance of reactive oxygen species. Excessively synthesized reactive oxygen species will damage the plant membrane system, causing great harm to crop physiology, and finally causing crop yield reduction.

The root hypoxia caused by waterlogging will weaken the respiration of plant roots, affect plant water metabolism and the absorption of mineral elements such as potassium ions; and the reduction of potassium ion levels will in turn lead to the closure of stomata and affect water absorption. Insufficient oxygen in plant roots leads to weakening of aerobic respiration and strengthening of anaerobic respiration. The enhancement of anaerobic respiration caused by anoxic environment causes the accumulation of toxic substances such as ethanol in plant roots, causing poisonous effects on plant root cells. In addition, the activities of plant photorespiratory enzymes and carboxylases are affected, exacerbating photoinhibition and affecting photosynthetic yield. Therefore, it is imminent to improve the waterlogging stress resistance of cultivated plants, which is of great significance in agricultural production.

Excessive active oxygen produced under waterlogging stress is an important factor causing plant damage. Therefore, in recent years, people try to alleviate the damage caused by waterlogging stress to plants from the perspective of reducing or eliminating the excessive active oxygen produced under adversity stress. Studies have shown that 8-hydroxyquinoline (superoxide radical scavenger), sodium benzoate (hydroxyl radical scavenger), vitamin E (singlet oxygen scavenger), etc. can effectively scavenge active oxygen and improve the waterlogging resistance of plants. damage ability. Ascorbic acid and reduced glutathione are also effective in scavenging a variety of reactive oxygen species. In addition, these active oxygen scavengers can also promote the activity of various antioxidant enzymes, thereby further scavenging active oxygen and protecting plants from active oxygen damage. In order to remove reactive oxygen species (reactive oxygen species, hereinafter referred to as ROS) caused by stress in time, plants have a series of anti-oxidation mechanisms to ensure their adaptation to various environmental stresses. Studies have shown that the tolerance of plants to abiotic stress is closely related to the ability of their antioxidant system to scavenge ROS. However, in agricultural production, the antioxidant mechanism in plants is often insufficient to remove stress-induced ROS in a timely manner, thus hindering plant growth.

Melatonin, also known as melatonin, melatonin, pineal gland, chemically named N-acetyl-5-methoxytryptamine, is an important plant endogenous active oxygen scavenger discovered only in recent years However, no one has studied the effect of melatonin on plant waterlogging stress.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the deficiencies of the above-mentioned prior art, and provide a new application of melatonin in improving plant resistance to waterlogging stress, which can significantly enhance the ability of plants to remove ROS, and reduce the impact of waterlogging stress on plants. The oxidative damage caused by the plant significantly improved the ability of plants to resist waterlogging stress.

To achieve the above object, the present invention adopts the following technical solutions:

New application of products with melatonin as active ingredient in improving plant resistance to waterlogging stress.

The product with melatonin as an active ingredient is an aqueous solution of melatonin.

The concentration of the melatonin aqueous solution is 10-300 μmol/L.

Preferably, the concentration of the melatonin aqueous solution is 50-200 μmol/L.

The melatonin aqueous solution is formed by diluting 100mmol/L melatonin ethanol solution.

The plant is a monocot or a dicot.

Preferably, the monocotyledonous plant is wheat, corn, rice or leek; the dicotyledonous plant is kaleidoscope, soybean, peanut or radish.

The method for improving plant resistance to waterlogging stress includes the following steps: spraying a product containing melatonin as an active ingredient on the leaves of plant seedlings.

In the spraying step, the spraying frequency is once every two days, and the total spraying frequency is 5-10 times.

The spraying amount of each plant is the same.

The structure of the melatonin is:

The beneficial effects of the present invention are: the present invention applies melatonin to improve plant resistance to waterlogging stress, can significantly enhance the ability of plants to remove ROS, reduce the oxidative damage caused by waterlogging stress to plants, and significantly improve plant resistance to waterlogging stress ability, thereby reducing the yield and quality decline caused by waterlogging; in addition, it is convenient to apply and has good stability.

detailed description

The present invention will be further described below in conjunction with embodiment.

The materials and reagents used in the following examples are commercially available unless otherwise specified.

The melatonin used in the following examples is a 100 mmol/L melatonin ethanol solution prepared by the Fruit Tree Department of China Agricultural University.

The melatonin product used in the following examples is an aqueous solution of melatonin.

Example 1, the new application of a product with melatonin as an active ingredient in improving the resistance to waterlogging stress of Semen chinensis.

Dilute the 100 mmol/L melatonin ethanol solution with distilled water to prepare respectively 50 μmol/L, 100 μmol/L, and 200 μmol/L melatonin aqueous solutions for use.

Sow the seeds of Betia chinensis soaked in deionized water for 48 hours in a pot filled with vermiculite, and cultivate them in a culture room with a temperature of 25 °C and a light intensity of 6 lux for two weeks, then treat them with half culture solution for one week, and then use full The nutrient solution was treated for one week.

Firstly, 24 seedlings of T. japonica with the same growth and four leaves and one heart were selected; then, the selected seedlings of T. chinensis were divided into four groups with 6 trees in each group; among them, three groups were the test group and one group was the control group.

Put the sterilized nutrient soil into wide-mouth glass bottles (diameter 6cm, depth 11cm), transplant the selected seedlings of T. chinensis into the above-mentioned wide-mouth glass bottles respectively, and submerge the nutrient soil with nutrient solution so that T. In the state of water, add about 10mL of nutrient solution every two days to keep the mountain stamens in a state of flooding.

Spray the prepared melatonin aqueous solution at the same time to the above-mentioned chrysanthemum transplanted into the wide-mouth glass bottle. Among them, the three experimental groups were sprayed with melatonin aqueous solution with a concentration of 50 μmol/L, 100 μmol/L, and 200 μmol/L respectively. , and the volume of the sprayed melatonin aqueous solution was the same, while the plants of the control group were sprayed with the same volume of the solvent used to prepare the melatonin aqueous solution, sprayed once every two days, and the total cycle was 9 days.

Measure the activity of malondialdehyde (MDA), melatonin, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in each group of plants before and after 9 days of treatment and count For analysis, the experiment was repeated three more times.

Analysis of the results: after 9 days of waterlogging treatment, the growth of Cedarwood not sprayed with melatonin solution was weak and showed obvious symptoms of waterlogging; however, with the increase of the concentration of sprayed melatonin solution, Cicada Cinnamomi resisted waterlogging stress 200μmol/L melatonin aqueous solution has the best effect on resisting waterlogging stress, and basically no symptoms of waterlogging appear. The above experiments show that exogenous spraying of melatonin aqueous solution can significantly improve the growth of St.

The MDA content in the plant is an index to measure the degree of plant oxidative damage. The higher the MDA content, the more serious the oxidative damage of the plant. Through the determination of the MDA content in the test plants, it was found that after 9 days of waterlogging stress treatment, the endogenous MDA content of the seedlings of Shandingzi seedlings increased significantly, from about 120nmol/g·FW to about 220nmol/g·FW, an increase of about 70%. . However, after treatment with exogenous 200μmol/L melatonin aqueous solution, the MDA content increased slightly compared with that before treatment. It shows that exogenous spraying of 200 μmol/L melatonin aqueous solution can effectively reduce the content of endogenous MDA under waterlogging stress.

Under waterlogging stress, the seedlings of T. japonicus were treated with melatonin aqueous solutions at concentrations of 50 μmol/L, 100 μmol/L, and 200 μmol/L for 9 days, and it was found that with the increase of the concentration of melatonin aqueous solution, the SOD in T. japonicus seedlings The activities of , POD and CAT were enhanced in turn. That is, under waterlogging stress, the degree of oxidative damage to plants sprayed with melatonin aqueous solution was significantly lower than that of plants not sprayed with melatonin aqueous solution. Stress-induced ROS.

The test data showed that the endogenous melatonin content of Shandingzi was significantly increased under waterlogging stress, from about 40ng/g·FW to about 150ng/g·FW, an increase of about 3 times. Under waterlogging stress, exogenous spraying of 200 μmol/L melatonin aqueous solution can also significantly increase the endogenous melatonin content of the seedlings of T. chinensis, from about 40 ng/g·FW to about 170 ng/g·FW , increased by about 3.2 times. The above shows that the exogenous spraying of melatonin solution will have a certain impact on the endogenous melatonin content of the seedlings of T. As a highly active antioxidant, melatonin can effectively scavenge the massively increased ROS caused by waterlogging, and the stress resistance of plants is closely related to its ability to scavenge ROS. Therefore, the increase of endogenous melatonin content shows that exogenous spraying of melatonin solution can effectively improve the ability of Shandingzi to remove ROS caused by waterlogging stress.

After 9 days of waterlogging treatment, the chlorophyll content in the leaves of Shandingzi leaves decreased significantly, from about 3.9mg/g·FW to about 1.4mg/g·FW, a drop of about 64%. However, after being treated with exogenous 200 μmol/L melatonin aqueous solution, the decline of chlorophyll content in stator leaves under waterlogging stress was significantly inhibited and maintained within a relatively normal range. After 9 days of waterlogging stress treatment, the photosynthetic rate of the leaves of C. chinensis decreased significantly, from about 1.4μmol·m-2·s-1 to about 0.7μmol·m-2·s-1, a drop of about 53%. However, after being treated with 200 μmol/L melatonin aqueous solution, the decrease in photosynthetic rate of stators under waterlogging stress was significantly alleviated, and normal photosynthesis was almost maintained, and the growth of the plants was almost the same as that of the control. It can be seen that spraying melatonin aqueous solution can effectively remove ROS, slow down the degradation of chlorophyll, and maintain the normal progress of photosynthesis under waterlogging stress.

The above experiments and data show that exogenous spraying of melatonin aqueous solution can significantly improve the ability of C. chinensis to resist waterlogging stress.

The content not described in detail in this specification belongs to the prior art known to those skilled in the art.

The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, so all equivalent changes or modifications made according to the structure, features and principles described in the patent scope of the present invention should be Including the patent application scope of the present invention.

Claims (7)

1. the product with epiphysin as active ingredient is improving the new opplication during plant resistant damage or crop failure caused by waterlogging is coerced；

The product with epiphysin as active ingredient is the epiphysin aqueous solution；

The concentration of the epiphysin aqueous solution is 10~300 μm of ol/L；

The epiphysin aqueous solution is formed by the epiphysin ethanol solution dilution of 100mmol/L.

2. new opplication as claimed in claim 1, it is characterised in that：The concentration of the epiphysin aqueous solution is 50~200 μm of ol/ L。

3. new opplication as claimed in claim 1, it is characterised in that：The plant is monocotyledon or dicotyledon.

4. new opplication as claimed in claim 3, it is characterised in that：The monocotyledon is wheat, corn, paddy rice or fragrant-flowered garlic Dish；The dicotyledon is Malus baccata, soybean, peanut or radish.

5. the new opplication as described in claim any one of 1-4, it is characterised in that：The side for improving plant resistant damage or crop failure caused by waterlogging stress Method, comprises the following steps：Product with epiphysin as active ingredient is sprayed on plantling blade face.

6. new opplication as claimed in claim 5, it is characterised in that：It is described to spray step, spray frequency once two days, total spray Number of times is applied for 5~10 times.

7. new opplication as claimed in claim 5, it is characterised in that：The amount of spraying of each each plant is identical.