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CN105008536A - 分离的寡核苷酸及其用途 - Google Patents

分离的寡核苷酸及其用途 Download PDF

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Publication number
CN105008536A
CN105008536A CN201480011125.2A CN201480011125A CN105008536A CN 105008536 A CN105008536 A CN 105008536A CN 201480011125 A CN201480011125 A CN 201480011125A CN 105008536 A CN105008536 A CN 105008536A
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CN
China
Prior art keywords
sequence
rvd
identification module
plasmid
oligonucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480011125.2A
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English (en)
Inventor
吴璐
许奇武
王磊
原辉
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BGI Technology Solutions Co Ltd
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BGI Technology Solutions Co Ltd
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Filing date
Publication date
Application filed by BGI Technology Solutions Co Ltd filed Critical BGI Technology Solutions Co Ltd
Priority to CN201480011125.2A priority Critical patent/CN105008536A/zh
Publication of CN105008536A publication Critical patent/CN105008536A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

本发明提出一种寡核苷酸及其用途。该分离的寡核苷酸包括：第一核酸分子，所述第一核酸分子编码双碱基识别模块，所述双碱基识别模块由串联的第一单碱基识别模块和第二单碱基识别模块构成，所述第一单碱基识别模块和第二单碱基识别模块均包含重复可变双氨基酸残基；第一扩增序列，所述第一扩增序列位于所述第一核酸分子的5’侧，并且所述第一扩增序列包含IIs型内切酶识别序列；以及第二扩增序列，所述第二扩增序列位于所述第一核酸分子的3’侧，并且所述第二核酸序列包含IIs型内切酶识别序列。能够快速获得各种不同组合的RVD，从而可以识别任意靶核酸序列。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201480011125.2A 2013-04-16 2014-04-15 分离的寡核苷酸及其用途 Pending CN105008536A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201480011125.2A CN105008536A (zh) 2013-04-16 2014-04-15 分离的寡核苷酸及其用途

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201310132864 2013-04-16
CN2013101328644 2013-04-16
CN201480011125.2A CN105008536A (zh) 2013-04-16 2014-04-15 分离的寡核苷酸及其用途
PCT/CN2014/075403 WO2014169810A1 (zh) 2013-04-16 2014-04-15 分离的寡核苷酸及其用途

Publications (1)

Publication Number Publication Date
CN105008536A true CN105008536A (zh) 2015-10-28

Family

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Family Applications (1)

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CN201480011125.2A Pending CN105008536A (zh) 2013-04-16 2014-04-15 分离的寡核苷酸及其用途

Country Status (3)

Country Link
CN (1) CN105008536A (zh)
HK (1) HK1214302A1 (zh)
WO (1) WO2014169810A1 (zh)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011072246A2 (en) * 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
WO2011146121A1 (en) * 2010-05-17 2011-11-24 Sangamo Biosciences, Inc. Novel dna-binding proteins and uses thereof
US20120204282A1 (en) * 2011-02-04 2012-08-09 Sangamo Biosciences, Inc. Methods and compositions for treating occular disorders
WO2012138927A2 (en) * 2011-04-05 2012-10-11 Philippe Duchateau Method for the generation of compact tale-nucleases and uses thereof
WO2012149470A1 (en) * 2011-04-27 2012-11-01 Amyris, Inc. Methods for genomic modification
WO2012152912A1 (en) * 2011-05-12 2012-11-15 Newvectys Genetically modified pig as a cancer prone model
CN102787125A (zh) * 2011-08-05 2012-11-21 北京大学 一种构建tale重复序列的方法
CN102864158A (zh) * 2012-09-29 2013-01-09 北京大学 用于基因定点修饰的tale重复片段的高效合成方法
WO2013016434A1 (en) * 2011-07-27 2013-01-31 The Board Institute, Inc. Compositions and methods of treating head and neck cancer

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011072246A2 (en) * 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
WO2011072246A3 (en) * 2009-12-10 2012-02-02 Regents Of The University Of Minnesota Tal effector-mediated dna modification
CN102770539A (zh) * 2009-12-10 2012-11-07 明尼苏达大学董事会 Tal效应子介导的dna修饰
WO2011146121A1 (en) * 2010-05-17 2011-11-24 Sangamo Biosciences, Inc. Novel dna-binding proteins and uses thereof
CN103025344A (zh) * 2010-05-17 2013-04-03 桑格摩生物科学股份有限公司 新型dna-结合蛋白及其用途
US20120204282A1 (en) * 2011-02-04 2012-08-09 Sangamo Biosciences, Inc. Methods and compositions for treating occular disorders
WO2012138927A2 (en) * 2011-04-05 2012-10-11 Philippe Duchateau Method for the generation of compact tale-nucleases and uses thereof
WO2012149470A1 (en) * 2011-04-27 2012-11-01 Amyris, Inc. Methods for genomic modification
WO2012152912A1 (en) * 2011-05-12 2012-11-15 Newvectys Genetically modified pig as a cancer prone model
WO2013016434A1 (en) * 2011-07-27 2013-01-31 The Board Institute, Inc. Compositions and methods of treating head and neck cancer
CN102787125A (zh) * 2011-08-05 2012-11-21 北京大学 一种构建tale重复序列的方法
CN102864158A (zh) * 2012-09-29 2013-01-09 北京大学 用于基因定点修饰的tale重复片段的高效合成方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIXIN LI等: "Rapid and highly efficient construction of TALE-basedtranscriptional regulators and nucleases for genomemodification", 《PLANT MOL BIOL》 *

Also Published As

Publication number Publication date
HK1214302A1 (zh) 2016-07-22
WO2014169810A1 (zh) 2014-10-23

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