CN104970201A - Production method for fermented soybean meal rich in arginine and lysine - Google Patents
Production method for fermented soybean meal rich in arginine and lysine Download PDFInfo
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- CN104970201A CN104970201A CN201510417312.7A CN201510417312A CN104970201A CN 104970201 A CN104970201 A CN 104970201A CN 201510417312 A CN201510417312 A CN 201510417312A CN 104970201 A CN104970201 A CN 104970201A
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- dregs
- lysine
- beans
- soybean meal
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- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 239000004472 Lysine Substances 0.000 title claims abstract description 42
- 235000019764 Soybean Meal Nutrition 0.000 title claims abstract description 30
- 239000004455 soybean meal Substances 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 29
- 239000004475 Arginine Substances 0.000 title claims abstract description 24
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 91
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 91
- 238000000855 fermentation Methods 0.000 claims description 42
- 230000001954 sterilising effect Effects 0.000 claims description 35
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 30
- 230000004151 fermentation Effects 0.000 claims description 24
- 239000002002 slurry Substances 0.000 claims description 21
- CANRESZKMUPMAE-UHFFFAOYSA-L Zinc lactate Chemical compound [Zn+2].CC(O)C([O-])=O.CC(O)C([O-])=O CANRESZKMUPMAE-UHFFFAOYSA-L 0.000 claims description 19
- 235000000193 zinc lactate Nutrition 0.000 claims description 19
- 239000011576 zinc lactate Substances 0.000 claims description 19
- 229940050168 zinc lactate Drugs 0.000 claims description 19
- 239000002994 raw material Substances 0.000 claims description 18
- PMVSDNDAUGGCCE-TYYBGVCCSA-L Ferrous fumarate Chemical compound [Fe+2].[O-]C(=O)\C=C\C([O-])=O PMVSDNDAUGGCCE-TYYBGVCCSA-L 0.000 claims description 17
- 235000002332 ferrous fumarate Nutrition 0.000 claims description 17
- 239000011773 ferrous fumarate Substances 0.000 claims description 17
- 229960000225 ferrous fumarate Drugs 0.000 claims description 17
- 235000015393 sodium molybdate Nutrition 0.000 claims description 17
- 239000011684 sodium molybdate Substances 0.000 claims description 17
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 17
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 15
- 229960003390 magnesium sulfate Drugs 0.000 claims description 15
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 15
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 15
- 235000019157 thiamine Nutrition 0.000 claims description 15
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims description 15
- 229960003495 thiamine Drugs 0.000 claims description 15
- 239000011721 thiamine Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000002361 compost Substances 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 241000194108 Bacillus licheniformis Species 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 8
- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 claims description 7
- 229940004120 bifidobacterium infantis Drugs 0.000 claims description 7
- 241001465754 Metazoa Species 0.000 abstract description 28
- 150000001413 amino acids Chemical class 0.000 abstract description 17
- 241000251468 Actinopterygii Species 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 6
- 238000009360 aquaculture Methods 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 235000013406 prebiotics Nutrition 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract 2
- 235000009697 arginine Nutrition 0.000 description 18
- 235000001014 amino acid Nutrition 0.000 description 16
- 230000000052 comparative effect Effects 0.000 description 8
- 239000003797 essential amino acid Substances 0.000 description 6
- 235000020776 essential amino acid Nutrition 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186012 Bifidobacterium breve Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 241001275898 Mylopharyngodon piceus Species 0.000 description 1
- 238000004497 NIR spectroscopy Methods 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000013527 bean curd Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000005792 cardiovascular activity Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
Based on a nutritional requirement of animals and arginine and lysine in current soybean meal can not satisfy the animal requirement, particularly the condition that the nutritional requirement of aquaculture animals limits growth of the animals, the invention aims to provide fermented soybean meal rich in arginine and lysine and a production method of the fermented soybean meal rich in arginine and lysine. The production method for fermented soybean meal provided by the invention can be used for effectively improving the content of arginine and lysine in soybean meal and improving the economical value of the soybean meal. Moreover, as far as feed production as concerned, the production cost can be lowered and the process can be simplified. As far as culture animals, particularly aquaculture animals and more particularly fishes, the demand of the fermented soybean meal on limiting amino acids can be satisfied. Furthermore, the fermented soybean meal has a prebiotic effect and can be used for improving the growing condition of the animals and the animal immunity.
Description
Technical field
The invention belongs to field of fodder, also relate to microbial fermentation technology simultaneously, be specifically related to a kind of production method being rich in the fermented bean dregs of arginine and lysine.
Background technology
For animal, amino acid can be divided into two classes, and a class is essential amino acid, and another kind of is nonessential amino acid; For feed manufacturing, in 10 kinds of essential amino acids that feed provides, if some amino acid lacks especially, then can limit the utilization rate of other essential amino acid, and determine the effective rate of utilization of whole feed protein, be called as limiting amino acid.Limiting amino acid in feed, according to the size of its restriction, is called first, second or the 3rd limiting amino acid.For each feed protein, first, second or the 3rd limiting amino acid of various animal may be different, for aquiculture animal, particularly fish, in fish meal, first, second limiting amino acid is arginine or lysine respectively, for pig, its first, second limiting amino acid is lysine or methionine.
Generally speaking, forage protein utilization rate can be improved by adding synthesizing amino acid in feed, if but do not add the first limiting amino acids, interpolation second or the 3rd limiting amino acid do not have effect.To this, one " bucket is theoretical " is had to do vivid explanation: 10 kinds of essential amino acids to be compared to the 10 pieces of planks surrounding a wooden barrel bucket wall, this bucket can fill how much water (being equivalent to effective trophic level of protein) depends on that the shortest block plank (being equivalent to the first limiting amino acids), and other plank is long also of no avail again.Lysine is the essential amino acid that animal body requirement is maximum, and animal body can not oneself synthesize completely, and particularly grow the requirement of animal to lysine high, the speed of growth is faster, and growth pressure is higher, then the lysine needed is also more.In most animals common feedstuffs raw material, content is lower, therefore is the first or second limiting amino acid, its effect be improve a poor appetite, enhancing development; Promote the healing of wound, fracture etc., strengthen the resistance to various infectious disease.Arginine is one of essential amino acid of fish, and it participates in protein in fish body, the synthesis of polyamines, growth promoting effects; Arginine has short endocrine effect; Arginine can generate nitric oxide in vivo, and nitric oxide participates in fish cardiovascular activity, the adjustment of immunologic function.
Summary of the invention
Can not meet Animal needs based on lysine and arginine in the nutritional need of animal and existing dregs of beans, the present situation of restriction growth of animal, the present invention aims to provide one and is rich in lysine and arginic fermented bean dregs and production method thereof.
For solving above technical problem, the present invention adopts following technical scheme:
Be rich in a production method for lysine and arginic fermented bean dregs, it is characterized in that, comprise the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.5 ~ 1.0;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 135 ~ 144 DEG C, and sterilization time is 2-3min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added sodium molybdate, zinc lactate, magnesium sulfate, thiamine (Cobastab
1) and ferrous fumarate, wherein the addition of sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.3-0.5%, 0.1-0.3%, 0.2-0.4%, 1-2% and 0.4-0.7% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen fermentation 36-48 hour, obtains bean pulp fermentation thing at 32-35 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, at 30-32 DEG C after anaerobic fermentation 24-30 hour, be cooled to 25-28 DEG C, continues anaerobic fermentation 36-48 hour, obtains tunning;
Step 7: the tunning obtained that ferments through step 6 is carried out drying, arginine and fermenting lysine dregs of beans must be rich in.
Preferably, the addition of sodium molybdate in described step 4, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.4%, 0.2%, 0.3%, 1.2% and 0.6% of dregs of beans weight.
Preferably, be at 33 DEG C, pass into oxygen ferment 40 hours in described step 5.
Preferably, be that anaerobic fermentation, after 28 hours, is cooled to 26 DEG C at 31 DEG C in described step 6, continue anaerobic fermentation 40 hours.
Preferably, low temperature drying is selected in the drying in described step 7, such as, dry at the temperature of 45 DEG C.
Tool of the present invention has the following advantages and beneficial effect:
First, the present invention adopts the method for biologically fermenting, by the composite fermentation of bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae, Lactobacillus plantarum and bifidobacterium breve, the nutritional labeling of dregs of beans is improved, effectively improves lysine and arginic content in dregs of beans.
Secondly, sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is with the addition of in fermentation substrate of the present invention, itself and dregs of beans carry out composite fermentation, effectively improve the arginic content in fermented bean dregs, five with the use of serving unexpected effect, it is compared with the scheme of not adding or partly add, substantially reduce fermentation time, also drastically increase lysine and arginic output simultaneously, this there is no report in the prior art, belongs to unforeseeable technique effect.
Again, by the fermented bean dregs that production method of the present invention is produced, it has been rich in lysine and arginine, no longer need additionally to strengthen lysine and arginine in the production of mixed feed using this dregs of beans, animal can be met, particularly aquatic livestock is to lysine and arginic demand, significantly reduces Feed Manufacturing cost, makes the production technology of feed be simplified simultaneously; In addition, the probiotic microorganisms that fermented bean dregs of the present invention uses microorganism all to belong to conventional, residual viable bacteria or spore can activate in animal intestinal, play certain prebiotic left and right; Meanwhile, create a large amount of vitamin by fermentable, particularly B family vitamin, contribute to upgrowth situation and the raising immunity of improving animal.
Generally speaking, the production method of fermented bean dregs of the present invention effectively can improve arginine and arginic content in dregs of beans, improve the economic worth of dregs of beans, simultaneously for Feed Manufacturing, it can reduce production cost, Simplified flowsheet, for cultivated animals, particularly aquiculture animal, particularly fish, it can meet the demand of animal to limiting amino acid, play prebiotic left and right simultaneously, improve the upgrowth situation of animal, improve animal dis motility rate.
Detailed description of the invention
embodiment 1: a kind of production method being rich in lysine and arginic fermented bean dregs, is characterized in that, comprise the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.8;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 144 DEG C, and sterilization time is 2min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added sodium molybdate, zinc lactate, magnesium sulfate, thiamine (Cobastab
1) and ferrous fumarate, wherein the addition of sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.4%, 0.2%, 0.3%, 1.2% and 0.6% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen and ferments 40 hours, obtain bean pulp fermentation thing at 33 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, anaerobic fermentation is after 28 hours at 31 DEG C, is cooled to 26 DEG C, continues anaerobic fermentation 40 hours, obtains tunning;
Step 7: the tunning obtained fermenting through step 6 carries out drying (drying at the temperature of 45 DEG C), must be rich in arginine and fermenting lysine dregs of beans.
embodiment 2: a kind of production method being rich in lysine and arginic fermented bean dregs, is characterized in that, comprise the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.8;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 135 DEG C, and sterilization time is 3min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added sodium molybdate, zinc lactate, magnesium sulfate, thiamine (Cobastab
1) and ferrous fumarate, wherein the addition of sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.3%, 0.3%, 0.2%, 2% and 0.4% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen and ferments 48 hours, obtain bean pulp fermentation thing at 32 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, anaerobic fermentation is after 30 hours at 30 DEG C, is cooled to 25 DEG C, continues anaerobic fermentation 48 hours, obtains tunning;
Step 7: the tunning obtained that ferments through step 6 is carried out drying, arginine and fermenting lysine dregs of beans must be rich in.
embodiment 3: a kind of production method being rich in lysine and arginic fermented bean dregs, is characterized in that, comprise the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.7;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 137 DEG C, and sterilization time is 2min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added sodium molybdate, zinc lactate, magnesium sulfate, thiamine (Cobastab
1) and ferrous fumarate, wherein the addition of sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.5%, 0.1%, 0.4%, 1% and 0.4% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen and ferments 36 hours, obtain bean pulp fermentation thing at 35 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, anaerobic fermentation is after 24 hours at 32 DEG C, is cooled to 28 DEG C, continues anaerobic fermentation 36 hours, obtains tunning;
Step 7: the tunning obtained that ferments through step 6 is carried out drying, arginine and fermenting lysine dregs of beans must be rich in.
embodiment 4: comparative example
comparative example 1: a kind of production method of fermented bean dregs, comprises the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.8;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 144 DEG C, and sterilization time is 2min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added zinc lactate, wherein the addition of zinc lactate is 0.2% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen and ferments 40 hours, obtain bean pulp fermentation thing at 33 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, anaerobic fermentation is after 28 hours at 31 DEG C, is cooled to 26 DEG C, continues anaerobic fermentation 40 hours, obtains tunning;
Step 7: the tunning obtained fermenting through step 6 carries out drying (drying at the temperature of 45 DEG C), to obtain final product.
comparative example 2: a kind of production method of fermented bean dregs, comprises the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.8;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 144 DEG C, and sterilization time is 2min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added sodium molybdate, zinc lactate and ferrous fumarate, wherein the addition of sodium molybdate, zinc lactate and ferrous fumarate is respectively 0.4%, 0.2% and 0.6% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen and ferments 40 hours, obtain bean pulp fermentation thing at 33 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, anaerobic fermentation is after 28 hours at 31 DEG C, is cooled to 26 DEG C, continues anaerobic fermentation 40 hours, obtains tunning;
Step 7: the tunning obtained fermenting through step 6 carries out drying (drying at the temperature of 45 DEG C), must be rich in arginine and fermenting lysine dregs of beans.
embodiment 5:
On the basis of the comparative examples of embodiment 1-3 and embodiment 4, method according to " in GB/T 18868-2002 feed moisture, thick protein, crude fibre, crude fat, lysine, methionine Fast Measurement near infrared spectroscopy " and " GB 28306-2012 national food safety standard food additives L-arginine " measures the content of arginine and lysine in fermented bean dregs, and concrete outcome is as following table:
| Processing sample | Lysine mass percentage | Arginine mass percentage |
| Raw material dregs of beans | 2.92 | 3.81 |
| Embodiment 1 | 11.27 | 14.74 |
| Embodiment 2 | 10.66 | 13.45 |
| Embodiment 3 | 10.13 | 12.97 |
| Comparative example 1 | 4.79 | 5.12 |
| Comparative example 2 | 5.21 | 6.37 |
From above measurement result, compare raw material dregs of beans, in the fermentation skin of beancurd of embodiment 1-3, lysine content improves 4-5 doubly, be up to 11.27%, arginic mass percentage improves 3-4 doubly left and right, is up to 14.74%, is rich in aquiculture animal in fermented bean dregs, lysine particularly needed for fish and arginine, effectively improve the nutritive value of dregs of beans.By the comparison of comparative example, also the guess of inventor and the result of fermentation test is on a small scale demonstrated, compound due to sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is added and is effectively improve lysine and arginic output, and this point never reports in prior art; From comparative result, the particularly interpolation of sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate, it greatly can improve lysine and arginic content.
embodiment 6:
At the product of fishery cultivating base trial edition inventive embodiments 1, the object of feeding be carp and black carp raise together with pond, compare through peasant household and feed, find the fish that the fish speed of growth adopting fermented bean dregs of the present invention to feed is fed faster than normal meal, and owing to not needing strengthening amino acid and enzyme, through adjusting, the production cost of feed per ton reduces about 4-8%, has greatly saved production cost; And through statistics, the survival rate that in net cage, the survival rate of fish compares traditional dregs of beans or commercially available fermented bean dregs or fish meal exceeds 3-7 percentage point, drastically increases the survival rate of feeding animals.
Raw material involved in the present invention is prior art can purchase available convenient source and bacterial classification.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after having read above-mentioned instruction content of the present invention.
Claims (6)
1. be rich in lysine and arginic fermented bean dregs and a production method thereof, it is characterized in that, comprise the following steps:
Step one: prepare dregs of beans raw material;
Step 2: add water and the soybean meal slurry that stirs to obtain in dregs of beans raw material, wherein expects that water weight ratio is: 1:0.5 ~ 1.0;
Step 3: soybean meal slurry step 2 obtained carries out sterilization treatment, sterilising temp is 135 ~ 144 DEG C, and sterilization time is 2-3min, naturally cools to normal temperature after sterilizing;
Step 4: the soybean meal slurry after step 3 sterilizing is added sodium molybdate, zinc lactate, magnesium sulfate, thiamine (Cobastab
1) and ferrous fumarate, wherein the addition of sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.3-0.5%, 0.1-0.3%, 0.2-0.4%, 1-2% and 0.4-0.7% of dregs of beans weight, and the dregs of beans compost that stirs to obtain;
Step 5: access bacillus subtilis, bacillus licheniformis bacillus and saccharomyces cerevisiae in the dregs of beans compost obtained through step 4, passes into oxygen fermentation 36-48 hour, obtains bean pulp fermentation thing at 32-35 DEG C;
Step 6: add Lactobacillus plantarum and bifidobacterium infantis in the bean pulp fermentation thing that obtains of fermenting through step 5, at 30-32 DEG C after anaerobic fermentation 24-30 hour, be cooled to 25-28 DEG C, continues anaerobic fermentation 36-48 hour, obtains tunning;
Step 7: the tunning obtained that ferments through step 6 is carried out drying, arginine and fermenting lysine dregs of beans must be rich in.
2. the production method being rich in lysine and arginic fermented bean dregs according to claim 1, it is characterized in that, the addition of sodium molybdate in described step 4, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively 0.4%, 0.2%, 0.3%, 1.2% and 0.6% of dregs of beans weight.
3. the production method being rich in lysine and arginic fermented bean dregs according to claim 1, is characterized in that, is at 33 DEG C, to pass into oxygen ferment 40 hours in described step 5.
4. the production method being rich in lysine and arginic fermented bean dregs according to claim 1, is characterized in that, is that anaerobic fermentation, after 28 hours, is cooled to 26 DEG C at 31 DEG C in described step 6, continues anaerobic fermentation 40 hours.
5. the production method being rich in lysine and arginic fermented bean dregs according to claim 1, is characterized in that, low temperature drying is selected in the drying in described step 7, such as, dry at the temperature of 45 DEG C.
6. be rich in lysine and arginic fermentation beans, it is characterized in that, it prepares through the method described in claim 1-5 any one.
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