CN104817371B - A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize - Google Patents
A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize Download PDFInfo
- Publication number
- CN104817371B CN104817371B CN201510144395.7A CN201510144395A CN104817371B CN 104817371 B CN104817371 B CN 104817371B CN 201510144395 A CN201510144395 A CN 201510144395A CN 104817371 B CN104817371 B CN 104817371B
- Authority
- CN
- China
- Prior art keywords
- selenium
- fermentation
- earthworm
- kelp
- fermentation material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 240000008042 Zea mays Species 0.000 title claims abstract description 86
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 86
- 238000002360 preparation method Methods 0.000 title claims abstract description 46
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title claims abstract description 28
- 235000009973 maize Nutrition 0.000 title claims abstract description 28
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 27
- 239000011669 selenium Substances 0.000 claims abstract description 139
- 238000000855 fermentation Methods 0.000 claims abstract description 134
- 230000004151 fermentation Effects 0.000 claims abstract description 134
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 111
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 110
- 239000000463 material Substances 0.000 claims abstract description 93
- 241000361919 Metaphire sieboldi Species 0.000 claims abstract description 77
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 70
- 241001494479 Pecora Species 0.000 claims abstract description 40
- 241000283690 Bos taurus Species 0.000 claims abstract description 36
- 239000002689 soil Substances 0.000 claims abstract description 35
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 18
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 13
- 241001655322 Streptomycetales Species 0.000 claims abstract description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 12
- 238000004321 preservation Methods 0.000 claims abstract description 3
- 229940091258 selenium supplement Drugs 0.000 claims description 106
- 241000894006 Bacteria Species 0.000 claims description 33
- 210000003608 fece Anatomy 0.000 claims description 25
- 239000010871 livestock manure Substances 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 24
- 239000000654 additive Substances 0.000 claims description 23
- 230000000996 additive effect Effects 0.000 claims description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 20
- 238000009395 breeding Methods 0.000 claims description 18
- 230000001488 breeding effect Effects 0.000 claims description 18
- 239000004576 sand Substances 0.000 claims description 12
- 239000002002 slurry Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 11
- 239000011781 sodium selenite Substances 0.000 claims description 11
- 229960001471 sodium selenite Drugs 0.000 claims description 11
- 235000015921 sodium selenite Nutrition 0.000 claims description 11
- 235000014655 lactic acid Nutrition 0.000 claims description 10
- 239000004310 lactic acid Substances 0.000 claims description 10
- 241000287828 Gallus gallus Species 0.000 claims description 7
- 239000004568 cement Substances 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 238000002803 maceration Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 230000000384 rearing effect Effects 0.000 claims description 6
- 241000230129 Eisenia <Phaeophyceae> Species 0.000 claims description 4
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 241000726221 Gemma Species 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000002893 slag Substances 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 5
- 235000011649 selenium Nutrition 0.000 description 95
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 58
- 235000005822 corn Nutrition 0.000 description 58
- 239000003337 fertilizer Substances 0.000 description 23
- 206010039921 Selenium deficiency Diseases 0.000 description 10
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- 229910052700 potassium Inorganic materials 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 6
- 229910052698 phosphorus Inorganic materials 0.000 description 6
- 239000011591 potassium Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- KFZAUHNPPZCSCR-UHFFFAOYSA-N iron zinc Chemical compound [Fe].[Zn] KFZAUHNPPZCSCR-UHFFFAOYSA-N 0.000 description 5
- 235000013336 milk Nutrition 0.000 description 5
- 239000008267 milk Substances 0.000 description 5
- 210000004080 milk Anatomy 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000011701 zinc Substances 0.000 description 5
- 229910052725 zinc Inorganic materials 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000005416 organic matter Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- OFQCQIGMURIECL-UHFFFAOYSA-N 2-[2-(diethylamino)ethyl]-2',6'-dimethylspiro[isoquinoline-4,4'-oxane]-1,3-dione;phosphoric acid Chemical compound OP(O)(O)=O.O=C1N(CCN(CC)CC)C(=O)C2=CC=CC=C2C21CC(C)OC(C)C2 OFQCQIGMURIECL-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 240000006153 Dioscorea trifida Species 0.000 description 2
- 235000002718 Dioscorea trifida Nutrition 0.000 description 2
- 241000243686 Eisenia fetida Species 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 229910017604 nitric acid Inorganic materials 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003342 selenium Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 150000003751 zinc Chemical class 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000019926 Keshan disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 235000014106 fortified food Nutrition 0.000 description 1
- 235000006486 human diet Nutrition 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- WALCGGIJOOWJIN-UHFFFAOYSA-N iron(ii) selenide Chemical compound [Se]=[Fe] WALCGGIJOOWJIN-UHFFFAOYSA-N 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000255 pathogenic effect Toxicity 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, preparation process is：The first step：Prepare kelp fermentation material；Second step：Prepare earthworm selenium-rich night soil；3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material：4th step：Continue to add bacillus megaterium, bacillus subtilis, black-koji mould, white light gray streptomycete progress secondary fermentation after kelp fermentation material and cattle and sheep excrement Selenium-enriched fermentation material are mixed with needle mushroom dreg, ferment and mixed fermentation material one is made；5th step：Air-dried after mixed fermentation material one is mixed with earthworm selenium-rich excrement and crush, pack preservation.The special Se-enriched bio-organic fertilizer of fresh edible maize being prepared using the method for the present invention, it can cultivate to obtain selenium-rich fresh edible maize, improve the economic benefit of the plantation of fresh edible maize, while improve the quality and yield of fresh edible maize, with good economy and zoology benifit, should be widely promoted.
Description
Technical field：
The present invention relates to agricultural fertilizer field, more particularly to a kind of preparation method of biological organic fertilizer, is specially a kind of fresh
Eat the preparation method of the special Se-enriched bio-organic fertilizer of corn.
Background technology：
Selenium is animal and the essential trace elements of the human body, has the function of various biological.According to statistics, the whole world has 2/3
Area is selenium deficiency Huo Dixi areas, and the county that China there are about 72% is in low selenium or Selenium deficiency area, wherein nearly 1/3 is serious selenium deficiency area.
Selenium deficiency can cause the generation of more than 40 kinds of selenium deficiency disease (such as Keshan disease), and the Se content only leaned in natural food is generally inadequate to completely
The normal need of sufficient human body is it is reported that edible selenium-enriched food is generally acknowledged most safe, most effective selenium-supply method.
Using cereal crops selenium content after selenium fertilizer than 3-32 times of control increase, fruit increases 2-4 times, vegetables increase 7-60
The report such as times remaining light brightness, Se content is far above vegetables and fruit in cereal and bean food.Absorption aspects in human body to selenium,
Biological source Organic Selenium is better than other Organic Seleniums, and the selenium in cereal accounts for the 70%-80% of selenium intake total amount in human diet, is human body
Most important selenium source, therefore develop selenium-rich cereal crops (such as rice, wheat, corn) and be of great significance.It is regional in selenium deficiency,
Feed and the Se content of cereal crops are improved by applying selenium, is the effective measures for preventing selenium deficiency.
Corn is the important cereal crops in China, its cultivated area and yield are only second to rice and wheat, occupy the 3rd.It is beautiful
Rice is C4 plant, and the dry of respiration consumption is few, and photosynthetic efficiency is higher, thus yield per unit area is higher, unit area
Yield is occupied first of world food crop.Fresh edible maize is also Green maize, that is, refers to the corn ear of milk stage, removes bract and fringe
Handle, boiling to ripe ready-to-serve corn.Fresh edible maize is the corresponding concept of dry corn with being dehydrated after complete ripeness, it
Feature is：Milk stage harvest is not dehydrated, processing or unprocessed, for edible corn.Fresh edible maize can be common jade
Rice, corn, waxy corn, high nutrition corn and characteristic odor type corn, are preferred with corn, waxy corn especially.Corn thin skin sweet tea
Tender, the fragrant glutinous exquisiteness of waxy corn meat, is liked by people very much.
Fresh edible maize nutritional ingredient and edible value had it compared with the dry seed of corn and fabricated product harvested after complete ripeness
And without too late.According to the sweet tea type for requiring harvesting, four class fresh corn of glutinous type, odor type and plain edition is eaten raw, various nutritional ingredients are all bright
It is aobvious to be better than the dry seed of conventional corn.Fresh edible maize contains a variety of amino acid, trace element, aliphatic acid etc., and nutrient categories reach several
Ten kinds.It is worth pointing out that content of starch is very high in the dry seed of conventional corn, considerably reduced in fresh edible maize；It is dry
The protein of inferior quality, is improved in seed；The nutrition such as the quite high vitamin of nutritive value, mineral matter and cellulose into
Point, content is all significantly increased in fresh edible maize.
Chinese invention patent CN103304345A discloses a kind of cultural method of selenium-rich zinc-iron corn, at corn heading stage
Apply selenium-rich zinc-iron chelating fertilizer into the soil of maize planting, the selenium-rich zinc-iron chelating fertilizer contains chelating selenium salt, chelate zinc salt
With chelating molysite, the chelating agent of the chelating selenium salt is amino acid, and the chelate zinc salt and the chelating agent for chelating molysite are second two
Amine tetraacethyl；The amount of application of the selenium-rich zinc-iron chelating fertilizer is in terms of the selenium in chelating fertilizer, zinc, ferro element, the amount of application of selenium element
For 0.8~1.5 gram/acre, the amount of application of Zn-ef ficiency is 80~150 grams/acre, and the amount of application of ferro element is 150~200 grams/acre.This
Invention also applies corn composite fertilizer while selenium-rich zinc-iron chelating fertilizer is applied.In the invention, compared to single administration containing one kind
Or control group, the control group of single administration corn composite fertilizer of the fertilizer of two kinds of elements (selenium, zinc, iron), using containing selenium, zinc, iron
Selenium, zinc, the iron content of the corn of the experimental group production of the fertilizer of three kinds of elements greatly improve.Invention addition is using chelating
The mode of fertilizer adds inorganic selenium, and Maize Absorption utilization rate is low, it is impossible to improves the content of selenium element in Corn well.
Chinese invention patent CN103304345A discloses a kind of system for the corn specific complex microorganism fertilizer for improveing soil
Preparation Method and products thereof, its preparation method are first by after fresh chicken manure and cattle manure deodorization, add thermophilic side born of the same parents mould, black song
Stalk, corn flour and potassium dihydrogen phosphate after mould, vinasse, brown sugar, crushing carry out fermenting twice, then air-dry to obtain preparation I；Again
Dregs of beans, wheat bran, calcium powder, bacillus megaterium, bacillus subtilis, lactic acid bacteria, sucrose and ferrous sulfate are taken through fermenting twice, and
Air-dry to obtain preparation II；Then preparation I and preparation II are mixed, then adds bacillus megaterium, bacillus subtilis, aspergillus niger
Bacterium, white light gray streptomycete, terrestrial cyanobacteria, photosynthetic bacteria and dinoflagellate, ferment, are eventually adding the chemical fertilizer needed for corn,
Corn specific complex microorganism fertilizer is obtained, the beneficial microbe of survival is contained in obtained product, soil aggregate structure can be improved
With other indexs, corn yield is improved.The invention is to improve soil as the main purpose, it is possible to increase corn yield, but do not have
It is improved the function of corn selenium content.
Chinese invention patent CN103583216A discloses a kind of High-Se corn cultural method and prepares High-Se corn nutrition
The method of powder, the High-Se corn cultural method, first choice selection corn variety, selection of land site preparation, plantation；In the phase of reeling off raw silk from cocoons, seed shape
An Organic Selenium is respectively sprayed into phase and milk stage.The invention can be such that High-Se corn Se content reaches more than 4mg/kg, Se content
Stablize, selenium-enriching cultivation method is easy to operate, of low cost, and selenium element in corn fruit is improved by way of spraying Organic Selenium
Content, selenium element absorptivity and conversion ratio and utilization rate are all relatively low.
The content of the invention：
In view of the problems of the existing technology, it is an object of the invention to provide a kind of special richness of fresh edible maize by the present invention
Selenium biological organic fertilizer, is converted into more conducively plant absorption by the inorganic selenium easily outflowed with water and utilizes and Se content high-efficient and lasting
State, cultivation obtain selenium-rich corn, improve the economic benefit of the plantation of fresh edible maize.
The present invention provides following technical solution：
A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, preparation process are：
The first step：Prepare kelp fermentation material；
Second step：Prepare earthworm selenium-rich night soil；
3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material：
4th step：Continue to add huge bud after kelp fermentation material and cattle and sheep excrement Selenium-enriched fermentation material are mixed with needle mushroom dreg
Spore bacillus, bacillus subtilis, black-koji mould, white light gray streptomycete carry out secondary fermentation, ferment and mixed fermentation material one is made；
5th step：Air-dried after mixed fermentation material one is mixed with earthworm selenium-rich excrement and crush, pack preservation.
Preferably, the preparation process of kelp fermentation material is in the first step：
Smash into the water that 8-10 times of kelp weight in wet base is added after kelp maceration to pieces kelp slurry, it is 5 to adjust kelp slurry pH, is added
Enter the pectase of kelp paste weight 0.4%, when enzymolysis 2 is small under the conditions of 47 DEG C of bath temperatures, after enzymolysis
It is 5.0 to adjust pH, and obtained kelp treats zymotic fluid, and bacillus megaterium is added in kelp treats zymotic fluid and is sent out with lactic acid bacteria
Ferment, fermentation time are 5-6 days, and fermentation temperature is 55-60 DEG C.
Preferably, the preparation process of the second step earthworm selenium-rich night soil is；
A. sodium selenite is added after chicken manure is dried, additive amount 400mg/kg, it is 60- to be uniformly mixed and adjust moisture
65%, earthworm breeding material is made；
B. it is respectively that the cement pit bottom of 1m × 1m × 0.5m spreads the fine sand of a thickness 1cm, fine sand surface paving in length, width and height
One gauze, then adds earthworm breeding material 125kg described in step A, puts earthworm breeding pond in order；
C. 2-2.2 kilograms of earthworm is inoculated with after rearing pool is put in order every other day, earthworm kind likes earthworm (Eisenia for compatriot
fetida)；
D. earthworm manure sample was collected every ten days, obtained earthworm selenium-rich excrement will be air-dried after the completion of earthworm manure sample collection
Just.
Preferably, the preparation process of the 3rd step cattle and sheep excrement Selenium-enriched fermentation material is：
Crushed after cattle and sheep excrement is dried, add sodium selenite, additive amount 200mg/kg, adjusts moisture after mixing
Content is 30-35%, and after being inoculated with thermophilic side born of the same parents mould, black-koji mould, inoculum concentration is 0.2% and 0.4%, fermentation temperature 58
DEG C, fermentation time is 10 days, and cattle and sheep excrement Selenium-enriched fermentation material is obtained after the completion of fermentation.
Preferably, it is according to parts by weight that 40-45 parts of kelp fermentation materials, 130-140 parts of cattle and sheep excrement are rich in the 4th step
Selenium fermentation material, 13-19 a part needle mushroom dreg are uniformly mixed, after mixing after continue add bacillus megaterium, bacillus subtilis,
Black-koji mould, white light gray streptomycete carry out secondary fermentation, and the additive amount of strain is respectively kelp fermentation material, sheep manure Selenium-enriched fermentation
Material, 0.2%, 0.3%, the 0.2% of needle mushroom dreg fermenting mixture quality, fermentation temperature are 30-32 DEG C, fermentation time 2-
3 days, fermentation finished obtained mixed fermentation material one.
Preferably, in the 5th step by mixed fermentation material one and earthworm selenium-rich excrement according to part by weight 100:1 mixing,
It is 15-18% to be air-dried after mixing to moisture, crushed 40 mesh sieves, pack preserves.
Preferably, the addition bacillus megaterium, lactic acid bacteria, thermophilic side born of the same parents mould, black-koji mould, bacillus subtilis
Bacterium, the additive amount of white light gray streptomycete are with living bacteria count gauge ,/gram of its living bacteria count amount >=500,000,000.
The characteristics of biological organic fertilizer：1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has the effect such as fixed nitrogen, phosphorus decomposing, potassium decomposing, can
To greatly improve the utilization rate of chemical fertilizer.2 improvement soil, increase soil fertility, and not only organic component content is rich in biological organic fertilizer
Richness, is in addition also conducive to the beneficial bacterium of soil environment improvement.After soil application biological organic fertilizer, largely add
The content of the nutriments such as the organic matter of soil, nitrogen, phosphorus, potassium, phosphorus decomposing, potassium solubilizing bacteria in fertilizer play in the soil its phosphorus decomposing,
Potassium decomposing acts on, and the P elements in soil and potassium element are discharged, add the available content of mineral substances of plant in soil.
On the other hand, since biological organic fertilizer is applied in soil, the organic matter content in soil is added, soil is become thin
Pine, nutrient laden, the original flora lived in the soil obtains amount reproduction, so as to ensure that the micro-ecological environment of soil.3 mitigate
Environmental pollution, according to statistics, has 1.4 hundred million tons to be incinerated, the burning of stalk in more than 600,000,000 tons of the agricultural crop straw that China produces every year
The waste of organic substance is not only caused, also creates air pollution.These discarded stalks are used and is fermented into biology and has
Machine fertilizer, it will the protection of huge economic benefit and air is produced to agricultural.It can improve soil quality using biological organic fertilizer
And soil microenvironment.4 improve crop qualities, and biological organic fertilizer is rich in the multiple nutritional components that have, and to impart fertilizer special
Fertilizer efficiency, has shown that through research, and biological organic fertilizer can improve the qualities such as color and luster, mouthfeel and the growth vigor of crop.5 mitigate crops
Insect pest, when the bacteriostasis of biological organic fertilizer itself, second, effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant promotion are made
With.
China's major part corn belongs to selenium deficiency or potential selenium deficiency type, and selenium most in conventional corn seed is
Easily it is absorbed by the body what is utilized.Therefore, it can be absorbed using corn and apply inorganic states selenium outside being enriched with, and be translated into peace
Entirely, the characteristic of effective biological organic state selenium, develops Se-enriched bio-organic fertilizer material of the present invention, is provided persistently for corn growth
Efficient selenium element, and reach its Se content highest when eating raw.
Beneficial effects of the present invention are：
1. the inorganic selenium easily outflowed with water is converted into more conducively by the present invention by the fermentation of earthworm and microorganism
Plant absorption utilizes and the state of content high-efficient and lasting, cultivation obtain selenium-rich corn, and selenium element content highest when eating raw, is improved
The economic benefit of the economic benefit of the plantation of corn, especially fresh edible maize.
2. the present invention contains a variety of effective microbes, retain good function characteristic possessed by general organic fertilizer, can promote
Into corn to nutrient absorb and tangerine resists the pathogenic effects of some pathogenic microorganisms, during mitigating corn planting
Pest and disease damage, improve plantation quality and yield, improve economy and zoology benifit.
3. selenium-rich of the present invention is efficient, selenium element can promote seed to sprout, improve root activity, promote plant growth, have
Effect improves plant quality.
Embodiment：
The embodiment of the present invention is described in detail below, the present embodiment is carried out lower premised on inventive technique scheme
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following realities
Apply example.The experimental program of actual conditions, the condition proposed by usually according to normal condition or manufacturer are not specified in embodiment
Implement.
Embodiment one
A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, preparation process are：
The first step：Prepare kelp fermentation material；
The preparation process of kelp fermentation material is：
Smash into the water that 8 times of kelp weight in wet base is added after kelp maceration to pieces kelp slurry, it is 5 to adjust kelp slurry pH, is added
The pectase of kelp paste weight 0.4%, when enzymolysis 2 is small under the conditions of 47 DEG C of bath temperatures, is adjusted after enzymolysis
It is 5.0 to save pH, and obtained kelp treats zymotic fluid, and bacillus megaterium is added in kelp treats zymotic fluid and is fermented with lactic acid bacteria,
Fermentation time is 5 days, and fermentation temperature is 55 DEG C.
Second step：Prepare earthworm selenium-rich night soil；
The preparation process of earthworm selenium-rich night soil is；
A. sodium selenite is added after chicken manure is dried, additive amount 400mg/kg, being uniformly mixed adjusting moisture is
60%, earthworm breeding material is made；
B. it is respectively that the cement pit bottom of 1m × 1m × 0.5m spreads the fine sand of a thickness 1cm, fine sand surface paving in length, width and height
One gauze, then adds earthworm breeding material 125kg described in step A, puts earthworm breeding pond in order；
C. 2 kilograms of earthworm is inoculated with after rearing pool is put in order every other day, earthworm kind loves earthworm for compatriot
(Eiseniafetida)；
D. earthworm manure sample was collected every ten days, obtained earthworm selenium-rich excrement will be air-dried after the completion of earthworm manure sample collection
Just.
3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material：
The preparation process of cattle and sheep excrement Selenium-enriched fermentation material is：
Crushed after cattle and sheep excrement is dried, add sodium selenite, additive amount 200mg/kg, adjusts moisture after mixing
Content is 30%, and after being inoculated with thermophilic side born of the same parents mould, black-koji mould, inoculum concentration is 0.2% and 0.4%, and fermentation temperature is 58 DEG C, hair
The ferment time is 10 days, and cattle and sheep excrement Selenium-enriched fermentation material is obtained after the completion of fermentation.
4th step：According to parts by weight by 40 parts of kelp fermentation materials, 130 portions of cattle and sheep excrement Selenium-enriched fermentation material, 13 portions of needle mushrooms
Bacteria residue is uniformly mixed, after mixing after continue add bacillus megaterium, bacillus subtilis, black-koji mould, white light gray streptomycete
Secondary fermentation is carried out, the additive amount of strain is respectively kelp fermentation material, sheep manure Selenium-enriched fermentation material, needle mushroom dreg fermenting mixture
0.2%, 0.3%, the 0.2% of quality, fermentation temperature are 30 DEG C, and fermentation time is 2 days, and fermentation finishes obtained mixed fermentation material
One；
5th step：Mixed fermentation material one is with earthworm selenium-rich excrement according to part by weight 100:1 mixing, air-dries after mixing to water
It is 15% to divide content, crushed 40 mesh sieves, pack preserves.
Embodiment two
A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, preparation process are：
The first step：Prepare kelp fermentation material；
The preparation process of kelp fermentation material is：
Smash into the water that 10 times of kelp weight in wet base is added after kelp maceration to pieces kelp slurry, it is 5 to adjust kelp slurry pH, is added
The pectase of kelp paste weight 0.4%, when enzymolysis 2 is small under the conditions of 47 DEG C of bath temperatures, is adjusted after enzymolysis
It is 5.0 to save pH, and obtained kelp treats zymotic fluid, and bacillus megaterium is added in kelp treats zymotic fluid and is fermented with lactic acid bacteria,
Fermentation time is 6 days, and fermentation temperature is 60 DEG C.
Second step：Prepare earthworm selenium-rich night soil；
The preparation process of earthworm selenium-rich night soil is；
A. sodium selenite is added after chicken manure is dried, additive amount 400mg/kg, being uniformly mixed adjusting moisture is
65%, earthworm breeding material is made；
B. it is respectively that the cement pit bottom of 1m × 1m × 0.5m spreads the fine sand of a thickness 1cm, fine sand surface paving in length, width and height
One gauze, then adds earthworm breeding material 125kg described in step A, puts earthworm breeding pond in order；
C. 2.2 kilograms of earthworm is inoculated with after rearing pool is put in order every other day, earthworm kind likes earthworm (Eisenia for compatriot
fetida)；
D. earthworm manure sample was collected every ten days, obtained earthworm selenium-rich excrement will be air-dried after the completion of earthworm manure sample collection
Just.
3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material：
The preparation process of cattle and sheep excrement Selenium-enriched fermentation material is：
Crushed after cattle and sheep excrement is dried, add sodium selenite, additive amount 200mg/kg, adjusts moisture after mixing
Content is 35%, and after being inoculated with thermophilic side born of the same parents mould, black-koji mould, inoculum concentration is 0.2% and 0.4%, and fermentation temperature is 58 DEG C, hair
The ferment time is 10 days, and cattle and sheep excrement Selenium-enriched fermentation material is obtained after the completion of fermentation.
4th step：According to parts by weight by 45 parts of kelp fermentation materials, 140 portions of cattle and sheep excrement Selenium-enriched fermentation material, 19 portions of needle mushrooms
Bacteria residue is uniformly mixed, after mixing after continue add bacillus megaterium, bacillus subtilis, black-koji mould, white light gray streptomycete
Secondary fermentation is carried out, the additive amount of strain is respectively kelp fermentation material, sheep manure Selenium-enriched fermentation material, needle mushroom dreg fermenting mixture
0.2%, 0.3%, the 0.2% of quality, fermentation temperature are 32 DEG C, and fermentation time is 3 days, and fermentation finishes obtained mixed fermentation material
One；
5th step：Mixed fermentation material one is with earthworm selenium-rich excrement according to part by weight 100:1 mixing, air-dries after mixing to water
It is 18% to divide content, crushed 40 mesh sieves, pack preserves.
Embodiment three
A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, preparation process are：
The first step：Prepare kelp fermentation material；
The preparation process of kelp fermentation material is：
Smash into the water that 9 times of kelp weight in wet base is added after kelp maceration to pieces kelp slurry, it is 5 to adjust kelp slurry pH, is added
The pectase of kelp paste weight 0.4%, when enzymolysis 2 is small under the conditions of 47 DEG C of bath temperatures, is adjusted after enzymolysis
It is 5.0 to save pH, and obtained kelp treats zymotic fluid, and bacillus megaterium is added in kelp treats zymotic fluid and is fermented with lactic acid bacteria,
Fermentation time is 5 days, and fermentation temperature is 58 DEG C.
Second step：Prepare earthworm selenium-rich night soil；
The preparation process of earthworm selenium-rich night soil is；
A. sodium selenite is added after chicken manure is dried, additive amount 400mg/kg, being uniformly mixed adjusting moisture is
63%, earthworm breeding material is made；
B. it is respectively that the cement pit bottom of 1m × 1m × 0.5m spreads the fine sand of a thickness 1cm, fine sand surface paving in length, width and height
One gauze, then adds earthworm breeding material 125kg described in step A, puts earthworm breeding pond in order；
C. 2.1 kilograms of earthworm is inoculated with after rearing pool is put in order every other day, earthworm kind likes earthworm (Eisenia for compatriot
fetida)；
D. earthworm manure sample was collected every ten days, obtained earthworm selenium-rich excrement will be air-dried after the completion of earthworm manure sample collection
Just.
3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material：
The preparation process of cattle and sheep excrement Selenium-enriched fermentation material is：
Crushed after cattle and sheep excrement is dried, add sodium selenite, additive amount 200mg/kg, adjusts moisture after mixing
Content is 33%, and after being inoculated with thermophilic side born of the same parents mould, black-koji mould, inoculum concentration is 0.2% and 0.4%, and fermentation temperature is 58 DEG C, hair
The ferment time is 10 days, and cattle and sheep excrement Selenium-enriched fermentation material is obtained after the completion of fermentation.
4th step：According to parts by weight by 43 parts of kelp fermentation materials, 135 portions of cattle and sheep excrement Selenium-enriched fermentation material, 16 portions of needle mushrooms
Bacteria residue is uniformly mixed, after mixing after continue add bacillus megaterium, bacillus subtilis, black-koji mould, white light gray streptomycete
Secondary fermentation is carried out, the additive amount of strain is respectively kelp fermentation material, sheep manure Selenium-enriched fermentation material, needle mushroom dreg fermenting mixture
0.2%, 0.3%, the 0.2% of quality, fermentation temperature are 31 DEG C, and fermentation time is 2 days, and fermentation finishes obtained mixed fermentation material
One；
5th step：Mixed fermentation material one is with earthworm selenium-rich excrement according to part by weight 100:1 mixing, air-dries after mixing to water
It is 16% to divide content, crushed 40 mesh sieves, pack preserves.
Example IV
A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, preparation process are：
The first step：Prepare kelp fermentation material；
The preparation process of kelp fermentation material is：
Smash into the water that 10 times of kelp weight in wet base is added after kelp maceration to pieces kelp slurry, it is 5 to adjust kelp slurry pH, is added
The pectase of kelp paste weight 0.4%, when enzymolysis 2 is small under the conditions of 47 DEG C of bath temperatures, is adjusted after enzymolysis
It is 5.0 to save pH, and obtained kelp treats zymotic fluid, and bacillus megaterium is added in kelp treats zymotic fluid and is fermented with lactic acid bacteria,
Fermentation time is 5 days, and fermentation temperature is 60 DEG C.
Second step：Prepare earthworm selenium-rich night soil；
The preparation process of earthworm selenium-rich night soil is；
A. sodium selenite is added after chicken manure is dried, additive amount 400mg/kg, being uniformly mixed adjusting moisture is
60%, earthworm breeding material is made；
B. it is respectively that the cement pit bottom of 1m × 1m × 0.5m spreads the fine sand of a thickness 1cm, fine sand surface paving in length, width and height
One gauze, then adds earthworm breeding material 125kg described in step A, puts earthworm breeding pond in order；
C. 2 kilograms of earthworm is inoculated with after rearing pool is put in order every other day, earthworm kind loves earthworm for compatriot
(Eiseniafetida)；
D. earthworm manure sample was collected every ten days, obtained earthworm selenium-rich excrement will be air-dried after the completion of earthworm manure sample collection
Just.
3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material：
The preparation process of cattle and sheep excrement Selenium-enriched fermentation material is：
Crushed after cattle and sheep excrement is dried, add sodium selenite, additive amount 200mg/kg, adjusts moisture after mixing
Content is 30%, and after being inoculated with thermophilic side born of the same parents mould, black-koji mould, inoculum concentration is 0.2% and 0.4%, and fermentation temperature is 58 DEG C, hair
The ferment time is 10 days, and cattle and sheep excrement Selenium-enriched fermentation material is obtained after the completion of fermentation.
4th step：According to parts by weight by 45 parts of kelp fermentation materials, 130 portions of cattle and sheep excrement Selenium-enriched fermentation material, 19 portions of needle mushrooms
Bacteria residue is uniformly mixed, after mixing after continue add bacillus megaterium, bacillus subtilis, black-koji mould, white light gray streptomycete
Secondary fermentation is carried out, the additive amount of strain is respectively kelp fermentation material, sheep manure Selenium-enriched fermentation material, needle mushroom dreg fermenting mixture
0.2%, 0.3%, the 0.2% of quality, fermentation temperature are 30 DEG C, and fermentation time is 3 days, and fermentation finishes obtained mixed fermentation material
One；
5th step：Mixed fermentation material one is with earthworm selenium-rich excrement according to part by weight 100:1 mixing, air-dries after mixing to water
It is 15% to divide content, crushed 40 mesh sieves, pack preserves.
The addition bacillus megaterium of embodiment one to four, lactic acid bacteria, thermophilic side born of the same parents mould, black-koji mould, withered grass bud
Spore bacillus, the additive amount of white light gray streptomycete with living bacteria count gauge, wherein EM bacteria agent number of viable >=20,000,000,000/gram, its
Remaining microbial inoculum number of viable >=500,000,000/gram.Used after purchase according to the specification application method, additive amount is converted into effective viable bacteria
Quantity add, such as purchase EM bacteria agent number of viable >=50,000,000,000/gram, then effective viable bacteria content of the high vigor bacteria preparation is
2.5 times of 20000000000/gram EM bacteria preparations, then its additive amount is a quarter of 20,000,000,000/gram EM bacteria preparations.
(weight is less than for potato harvest time-division yampi (weight is more than 150g), middle potato (weight 75-150g) and small potato
75g) three classes, weight/total output * 100% of commodity potato rate=(yampi+middle potato).
Planting experiment one
Test place：Laizhou City of Shandong Province.
Corn variety：Conventional corn kind Zheng Dan 958 (ZD958) and purple glutinous 218 (JN218) in glutinous corn variety capital.
For trying soil：Se content 0.05mg/kg, organic matter 7.08g/kg, alkali-hydrolyzable nitrogen 44.56mg/kg, rapid available phosphorus
46.32mg/kg available potassium 59.01mg/kg.
It is divided into 36 fritters for examination soil, random every three pieces divide one group into, are divided into 12 groups, and every six groups form one
A big group of corn variety.It is special that the fresh edible maize that the preparation method of embodiment one to four is prepared is applied respectively in each big group
With Se-enriched bio-organic fertilizer, the group for the treatment of group one to four is denoted as, amount of application is 70kg/ mus, is left one piece and applies in the market purchase
Corn bio-feritlizer, amount of application are applied according to purchase bio-feritlizer specification, are denoted as processing one, last block uses processing one
The bio-feritlizer and Spraying Se foliar fertilizer, usage amount and application method are referring to specification.
General planting mode and way to manage.
Selenium element measures:Weigh sample 0.25g to be placed in 150mL triangular flasks, enriching nitric acid (top pure grade) 8mL and hydrogen peroxide
2mL, cap upper surface ware, heats (180 DEG C) resolutions to limpid, such as not clear, continues to add hydrogen peroxide transparent to solution.It is cold
But, 50mL is settled to ultra-pure water transfer, shaken up to be measured.Selenium element is surveyed on plasma mass spectrograph (Elan6000 types)
It is fixed, 3 repetitions.
Milk stage, harvests fresh fringe, surveys selenium element content in corn yield and fresh fringe, and statistical result is shown in Table one.
Table one：Selenium element content in one corn yield of planting experiment and fresh fringe
Data are expressed in a manner of average ± variance.
Planting experiment two
Test place：Changchun City.
Corn variety：Lucky oil 1, lucky single 342.
For trying soil：Se content 0.01mg/kg, organic matter 2.2%, ammonium nitrogen 45.7mg/L, available P, K, Zn and S
Respectively 13.1,66.5,2.3 and 17.4mg/L.
It is divided into 36 fritters for examination soil, random every three pieces divide one group into, are divided into 12 groups, and every six groups form one
A big group of corn variety.It is special that the fresh edible maize that the preparation method of embodiment one to four is prepared is applied respectively in each big group
With Se-enriched bio-organic fertilizer, the group for the treatment of group one to four is denoted as, amount of application is 22kg/ mus, is left one piece and applies in the market purchase
Corn bio-feritlizer, amount of application are applied according to purchase bio-feritlizer specification, are denoted as processing one, last block uses processing one
The bio-feritlizer and Spraying Se foliar fertilizer, usage amount and application method are referring to specification.
General planting mode and way to manage.
Selenium element measures:Weigh sample 0.25g to be placed in 150mL triangular flasks, enriching nitric acid (top pure grade) 8mL and hydrogen peroxide
2mL, cap upper surface ware, heats (180 DEG C) resolutions to limpid, such as not clear, continues to add hydrogen peroxide transparent to solution.It is cold
But, 50mL is settled to ultra-pure water transfer, shaken up to be measured.Selenium element is surveyed on plasma mass spectrograph (Elan6000 types)
It is fixed, 3 repetitions.
Milk stage, harvests fresh fringe, surveys selenium element content in corn yield and fresh fringe, and statistical result is shown in Table two.
Data are expressed in a manner of average ± variance.
Above content is only the better embodiment of the present invention, for those of ordinary skill in the art, according to the present invention
Thought, there will be changes, this specification content should not be construed as to this hair in specific embodiments and applications
Bright limitation.
Claims (1)
1. a kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize, it is characterised in that preparation process is：
The first step：Prepare kelp fermentation material；
Second step：Prepare earthworm selenium-rich night soil；
3rd step：Prepare cattle and sheep excrement Selenium-enriched fermentation material；
4th step：Continue to add huge gemma bar after kelp fermentation material and cattle and sheep excrement Selenium-enriched fermentation material are mixed with needle mushroom dreg
Bacterium, bacillus subtilis, black-koji mould, white light gray streptomycete carry out secondary fermentation, ferment and mixed fermentation material one is made；
5th step：Air-dried after mixed fermentation material one is mixed with earthworm selenium-rich excrement and crush, pack preservation；
Wherein, the preparation process of kelp fermentation material is in the first step：8-10 times of kelp weight in wet base will be added after kelp maceration
Water smashs into kelp slurry to pieces, and it is 5 to adjust kelp slurry pH, the pectase of kelp paste weight 0.4% is added, in 47 DEG C of water-bath temperature
It is 5.0 to adjust pH when enzymolysis 2 is small under the conditions of degree, after enzymolysis, and obtained kelp treats zymotic fluid, waits to ferment in kelp
Add bacillus megaterium in liquid to ferment with lactic acid bacteria, fermentation time is 5-6 days, and fermentation temperature is 55-60 DEG C；
Wherein, the preparation process of the second step earthworm selenium-rich night soil is；
A. sodium selenite is added after chicken manure is dried, additive amount 400mg/kg, it is 60- to be uniformly mixed and adjust moisture
65%, earthworm breeding material is made；
B. it is respectively that the cement pit bottom of 1m × 1m × 0.5m spreads the fine sand of a thickness 1cm in length, width and height, fine sand surface spreads one
Gauze, then adds earthworm breeding material 125kg described in step A, puts earthworm breeding pond in order；
C. 2-2.2 kilograms of earthworm is inoculated with after rearing pool is put in order every other day, earthworm kind likes earthworm (Eisenia for compatriot
fetida)；
D. earthworm manure sample was collected every ten days, obtained earthworm selenium-rich excrement will be air-dried after the completion of earthworm manure sample collection；
Wherein, the preparation process of the 3rd step cattle and sheep excrement Selenium-enriched fermentation material is：Crushed after cattle and sheep excrement is dried, add sub- selenium
Sour sodium, additive amount 200mg/kg, it is 30-35% to adjust moisture after mixing, is inoculated with thermophilic side born of the same parents mould, aspergillus niger
Bacterium, inoculum concentration are 0.2% and 0.4%, and fermentation temperature is 58 DEG C, and fermentation time is 10 days, and cattle and sheep excrement richness is obtained after the completion of fermentation
Selenium fermentation material；
Wherein, in the 4th step according to parts by weight by 40-45 parts of kelp fermentation materials, 130-140 parts of cattle and sheep excrement Selenium-enriched fermentations
Material, 13-19 part needle mushroom dregs be uniformly mixed, after mixing after continue addition bacillus megaterium, bacillus subtilis, aspergillus niger
Bacterium, white light gray streptomycete carry out secondary fermentation, and the additive amount of strain is respectively kelp fermentation material, cattle and sheep excrement Selenium-enriched fermentation material, gold
0.2%, 0.3%, the 0.2% of pin mushroom slag fermenting mixture quality, fermentation temperature are 30-32 DEG C, and fermentation time is 2-3 days,
Fermentation finishes obtained mixed fermentation material one；
Wherein, in the 5th step by mixed fermentation material one and earthworm selenium-rich excrement according to part by weight 100:1 mixing, after mixing
It is 15-18% to air-dry to moisture, crushed 40 mesh sieves, pack preserves；
Wherein, the addition bacillus megaterium, lactic acid bacteria, thermophilic side born of the same parents mould, black-koji mould, bacillus subtilis, white shallow
The additive amount of grey streptomycete is with living bacteria count gauge ,/gram of its living bacteria count amount >=500,000,000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510144395.7A CN104817371B (en) | 2015-03-30 | 2015-03-30 | A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510144395.7A CN104817371B (en) | 2015-03-30 | 2015-03-30 | A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104817371A CN104817371A (en) | 2015-08-05 |
| CN104817371B true CN104817371B (en) | 2018-05-11 |
Family
ID=53727881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510144395.7A Active CN104817371B (en) | 2015-03-30 | 2015-03-30 | A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104817371B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105777247B (en) * | 2016-01-28 | 2019-09-17 | 中国农业大学 | A kind of production method of selenium-enriched earthworm organic fertilizer |
| CN116199544A (en) * | 2023-02-21 | 2023-06-02 | 沧州市农林科学院 | A kind of selenium-enriched active organic fertilizer in saline-alkali land and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602621A (en) * | 2009-06-25 | 2009-12-16 | 山东农业大学 | Production system of selenium-enriched product based on recycling ecological agriculture |
| CN102731176A (en) * | 2012-06-28 | 2012-10-17 | 威海市世代海洋生物科技有限公司 | Production method for seaweed bio-composite organic liquid fertilizer |
| CN103396229A (en) * | 2013-07-31 | 2013-11-20 | 常熟市常福有机复合肥有限公司 | Selenium-enriched biological organic fertilizer |
| CN104030769A (en) * | 2014-06-30 | 2014-09-10 | 安徽利海新城镇开发有限公司 | Wormcast organic solid fertilizer and preparation method thereof |
| CN104193529A (en) * | 2014-08-29 | 2014-12-10 | 青岛嘉禾丰肥业有限公司 | Preparation method of selenium-rich organic fertilizer |
| CN104370611A (en) * | 2014-11-19 | 2015-02-25 | 马英臣 | Special fertilizer for orchids |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524224A (en) * | 2013-10-15 | 2014-01-22 | 淮北师范大学 | Selenium-rich organic fertilizer based on wormcast |
-
2015
- 2015-03-30 CN CN201510144395.7A patent/CN104817371B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602621A (en) * | 2009-06-25 | 2009-12-16 | 山东农业大学 | Production system of selenium-enriched product based on recycling ecological agriculture |
| CN102731176A (en) * | 2012-06-28 | 2012-10-17 | 威海市世代海洋生物科技有限公司 | Production method for seaweed bio-composite organic liquid fertilizer |
| CN103396229A (en) * | 2013-07-31 | 2013-11-20 | 常熟市常福有机复合肥有限公司 | Selenium-enriched biological organic fertilizer |
| CN104030769A (en) * | 2014-06-30 | 2014-09-10 | 安徽利海新城镇开发有限公司 | Wormcast organic solid fertilizer and preparation method thereof |
| CN104193529A (en) * | 2014-08-29 | 2014-12-10 | 青岛嘉禾丰肥业有限公司 | Preparation method of selenium-rich organic fertilizer |
| CN104370611A (en) * | 2014-11-19 | 2015-02-25 | 马英臣 | Special fertilizer for orchids |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104817371A (en) | 2015-08-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105481580B (en) | A kind of Kiwi fertilizer special for organic and preparation method thereof | |
| CN104844313B (en) | A kind of preparation method of selenium-rich cherry and tomato bio-organic fertilizer special | |
| Oke | Cassava as food in Nigeria | |
| CN106748247A (en) | A kind of selenium-enriched foliage fertilizer and preparation method thereof | |
| CN104817368B (en) | A kind of preparation method for the biological organic fertilizer that can improve Fuji apple flavor quality | |
| CN101935260A (en) | Plant-derived growth-promoting organic medical fertilizer and preparation method thereof | |
| CN102910970A (en) | Method for preparing multifunctional Chinese medicinal organic bacterial manure | |
| CN107226761A (en) | A kind of selenium-rich black soya bean bio-feritlizer and preparation method thereof | |
| KR101383550B1 (en) | Growth-promoting rice using effective micro-organisms and cultivation method thereof | |
| CN105110945A (en) | Enoki mushroom culture substrate and preparation method thereof | |
| CN107698398A (en) | A kind of Rice Cropping organic fertilizer | |
| CN107324940A (en) | A kind of dragon fruit synergy fertilizer and preparation method thereof | |
| CN105198556A (en) | Microbial fertilizer capable of improving sweetness of oranges and preparation method thereof | |
| CN106242801A (en) | A kind of cultural method of selenium-rich Pleurotus eryngii | |
| KR100679665B1 (en) | Nutrients composition for fruit tree cultivation containing selenium and method of cultivating fruit tree containing selenium | |
| CN108164334A (en) | A kind of high-yield planting method of selenium-rich sugar orange fertilizer and sugar orange | |
| CN107417443A (en) | A kind of high-yield planting method of selenium rich kiwi fruit fertilizer and Kiwi berry | |
| CN104844285B (en) | A kind of preparation method for the biological organic fertilizer improving cherry and tomato immunity | |
| CN107517714A (en) | A kind of cultural method of high yield high nutrition strawberry | |
| CN104817371B (en) | A kind of preparation method of the special Se-enriched bio-organic fertilizer of fresh edible maize | |
| CN107027620A (en) | It is a kind of while the selection of the plant rich in selenium and gamma aminobutyric acid | |
| CN102037894A (en) | Method for breeding and screening astragalus sinicus varieties with high selenium content | |
| CN105766578B (en) | Mushroom bran stem of noble dendrobium quality imitating wild planting process | |
| CN104817366B (en) | A kind of preparation method for the biological organic fertilizer that can improve Fuji apple storage quality | |
| CN104817349A (en) | Preparation method of zinc-iron-rich special biological organic fertilizer for fresh eating corn |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180929 Address after: 713800 Xianyang County, Shaanxi Province Patentee after: Shaanxi Bo Qin biotechnology company limited Address before: 215000 398 Jinyang Road, Wang Ting Town, Xiangcheng District, Suzhou, Jiangsu Patentee before: Suzhou Rencheng Bio-Science & Technology Co., Ltd. |