CN104803523B - The continuous harvesting apparatus of microalgae and method thereof associated with a kind of electricity flocculation-flotation - Google Patents
The continuous harvesting apparatus of microalgae and method thereof associated with a kind of electricity flocculation-flotation Download PDFInfo
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- 238000003860 storage Methods 0.000 claims description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 229910052742 iron Inorganic materials 0.000 claims description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims 1
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- 238000005189 flocculation Methods 0.000 abstract description 11
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- 238000009297 electrocoagulation Methods 0.000 abstract description 5
- 238000005265 energy consumption Methods 0.000 abstract description 5
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
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- 235000014413 iron hydroxide Nutrition 0.000 abstract description 2
- NCNCGGDMXMBVIA-UHFFFAOYSA-L iron(ii) hydroxide Chemical compound [OH-].[OH-].[Fe+2] NCNCGGDMXMBVIA-UHFFFAOYSA-L 0.000 abstract description 2
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- 229910052782 aluminium Inorganic materials 0.000 description 2
- 238000009300 dissolved air flotation Methods 0.000 description 2
- 238000005868 electrolysis reaction Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 1
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- 238000000703 high-speed centrifugation Methods 0.000 description 1
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- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
- 229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
- 239000000347 magnesium hydroxide Substances 0.000 description 1
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- Water Treatment By Electricity Or Magnetism (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
一种电絮凝‑气浮联用的微藻连续收获装置及其方法，包含依次相互连接的电絮凝气浮区、收获区、出水区和气浮系统。将藻液与苦卤水混合物流经一个跑道池充分混合，进入在跑道池的末端的深槽；待收藻类从藻液入口泵入并上升到电解板，被电极释放出来的铁的氢氧化物胶体所絮凝；被絮凝团聚的微藻细胞团，被溶气释放口释放的微气泡吸附上浮，并促使微藻细胞团进一步团聚，流入收获区，经刮藻机刮入储藻池，送入脱水烘干工序；收获区底部的清水，通过挡板二下部的空隙进入出水区。本发明大大提高了微藻的收获效率。该装置投资少，占地面积小，操作简单，具有低能耗高效率，环保的优点，适合大规模生产。
An electrocoagulation-air flotation combined microalgae continuous harvesting device and method thereof, comprising an electrocoagulation air flotation area, a harvesting area, a water outlet area and an air flotation system connected in sequence. The mixture of algae liquid and bittern water flows through a runway pool and is fully mixed, and enters the deep groove at the end of the runway pool; the algae to be harvested are pumped in from the algae liquid inlet and rise to the electrolytic plate, and the iron hydroxide released by the electrode The colloid is flocculated; the microalgae cell clusters agglomerated by flocculation are adsorbed and floated by the microbubbles released from the dissolved air release port, and the microalgae cell clusters are further agglomerated and flow into the harvesting area. Drying process; the clear water at the bottom of the harvesting area enters the water outlet area through the gap at the lower part of the second baffle plate. The invention greatly improves the harvesting efficiency of microalgae. The device has the advantages of low investment, small floor space, simple operation, low energy consumption, high efficiency and environmental protection, and is suitable for large-scale production.
Description
技术领域 technical field
本发明属于生物技术领域，涉及一种连续式微藻收获的装置及其方法。 The invention belongs to the field of biotechnology, and relates to a continuous microalgae harvesting device and a method thereof.
背景技术 Background technique
微藻的收获成本过高一直是制约其大规模生产的瓶颈问题之一，目前微藻收获的主要方法有膜过滤，离心及絮凝等。膜过滤法不仅生产效率很低，而且膜易污染堵塞，膜的再生或更新的成本也很高。因此不适合产业化生产应用。离心是较为常用的方法，虽无需添加其它化学试剂，但是操作复杂，能耗大，成本高。中国发明专利“一种管式离心机微藻收集装置（CN201220618371.2）”公开了一种管式离心机微藻收集装置，其技术方案要点是：借助离心过滤从藻液中排除液体，藻液被引入一快速旋转的网状套筒中，通过高速离心作用，微藻被留在多孔的网上，液体则受离心作用从网孔中挤出。尽管该装置结构简单，设置有可拆卸的卸料套筒，提高了微藻的收获率。但其微藻离心后需要卸料，拆洗和安装均需要时间，而且离心是一个能耗巨大的过程，无形中增加了微藻的收获成本，不利于工业化生产。 The high cost of harvesting microalgae has always been one of the bottlenecks restricting its large-scale production. At present, the main methods for harvesting microalgae include membrane filtration, centrifugation and flocculation. Membrane filtration not only has very low production efficiency, but also the membrane is easily fouled and clogged, and the cost of membrane regeneration or renewal is also high. Therefore it is not suitable for industrialized production application. Centrifugation is a more commonly used method. Although it does not need to add other chemical reagents, the operation is complicated, the energy consumption is large, and the cost is high. The Chinese invention patent "a microalgae collection device for a tubular centrifuge (CN201220618371.2)" discloses a microalgae collection device for a tubular centrifuge. The liquid is introduced into a fast-rotating mesh sleeve, and the microalgae are left on the porous net through high-speed centrifugation, and the liquid is squeezed out of the mesh by centrifugal force. Although the device has a simple structure, it is provided with a detachable discharge sleeve, which improves the harvest rate of microalgae. However, the microalgae needs to be unloaded after centrifugation, and it takes time to disassemble, wash and install. Moreover, centrifugation is a process with huge energy consumption, which virtually increases the harvesting cost of microalgae, which is not conducive to industrial production.
微藻的絮凝沉淀收获法近年来也得到重视，例如中国发明专利“一种微藻絮凝沉降收获方法（CN201410453206.X）”公开了一种利用季铵盐阳离子淀粉作为絮凝剂并结合搅拌的方式使微藻充分絮凝沉降到絮凝罐底部加以收集的方法。尽管该方法工艺简单，反应时间短，絮凝效果好；但是其所需絮凝剂量过多，不仅会增加成本，还会对微藻收获物以及水体造成一定的污染，每次絮凝都需要较长的静置时间，不能连续化生产。 The flocculation and sedimentation harvesting method of microalgae has also received attention in recent years. For example, the Chinese invention patent "A Microalgae Flocculation and Sedimentation Harvesting Method (CN201410453206.X)" discloses a method of using quaternary ammonium cationic starch as a flocculant combined with stirring It is a method of fully flocculating and settling microalgae to the bottom of the flocculation tank for collection. Although this method has simple process, short reaction time and good flocculation effect; however, too much flocculation dose is required, which will not only increase the cost, but also cause certain pollution to the microalgae harvest and water body, and each flocculation requires a long time. Standing time, can not be continuous production.
发明内容 Contents of the invention
本发明的目的是克服现有技术的不足，提供一种微藻电絮凝-气浮联用的微藻连续收获装置及其方法，实现环境友好的微藻连续收获方法，提高微藻的收获效率，降低其生产成本。 The purpose of the present invention is to overcome the deficiencies of the prior art, provide a microalgae electroflocculation-air flotation combined microalgae continuous harvesting device and method thereof, realize an environmentally friendly microalgae continuous harvesting method, and improve the harvesting efficiency of microalgae , to reduce its production cost.
本发明是通过以下技术方案实现的。 The present invention is achieved through the following technical solutions.
本发明所述的装置包括依次相互连接的电絮凝气浮区、收获区、出水区和气浮系统。 The device of the invention comprises an electric flocculation air flotation area, a harvest area, a water outlet area and an air flotation system which are interconnected in sequence.
所述的电絮凝气浮区由藻液入口（1），溶气释放口（2），电解板（3），挡板（4）组成。 The electroflocculation air flotation zone is composed of an algae liquid inlet (1), a dissolved air release port (2), an electrolytic plate (3) and a baffle (4).
所述的收获区由刮藻机（5），储藻池（6），收藻口（7），排渣口（8），挡板（9）组成。 The harvesting area is composed of an algae scraper (5), an algae storage pool (6), an algae collection port (7), a slag discharge port (8) and a baffle (9).
所述的出水区由溢水口（17），排水管（18）组成。 The water outlet area is composed of an overflow port (17) and a drainpipe (18).
所述的气浮系统由溶气泵进水管（10），蝶阀（11），溶气进气管（12），溶气泵（13），溶气罐（14），溶气压力表（15），溶气泵出液管（16）组成。 The air flotation system consists of dissolved air pump inlet pipe (10), butterfly valve (11), dissolved air inlet pipe (12), dissolved air pump (13), dissolved air tank (14), dissolved air pressure gauge (15), dissolved air Air pump liquid outlet pipe (16) is formed.
本发明装置各部分的连接关系是：藻液入口（1）和溶气释放口（2）位于电絮凝气浮区的最下方，溶气释放口（2）的上方是电解板（3），电解板（3）的后面是挡板一（4），挡板一（4）后面为收获区，藻液流过挡板（4）进入收获区，收获区顶部是刮藻机（5），刮藻机（5）后下方是储藻池（6），收藻口（7）位于储藻池（6）底部，排渣口（8）位于收获区底部，收获区后面是挡板二（9），挡板二（9）后面为出水区，清水通过挡板二（9）下面的空隙进入出水区，出水区中部是溢水口（17），上部是排水管道（18），底部是溶气泵进水口（10），溶气泵进水管（10）上安装有蝶阀（11），清水通过溶气泵进水口（10）进入到气浮系统，溶气泵进水管与溶气进气管（12）连接，再一同连接到溶气泵（13），溶气泵（13）上端连接溶气罐（14），下端连接溶气泵出液管（16），溶气泵出液管（16）上安装有溶气压力表（15），溶气泵出液管（16）继而与溶气释放口（2）相连。 The connection relationship of each part of the device of the present invention is: the algae liquid inlet (1) and the dissolved air release port (2) are located at the bottom of the electrocoagulation air flotation area, and the electrolytic plate (3) is above the dissolved air release port (2), Behind the electrolytic plate (3) is the baffle one (4), behind the baffle one (4) is the harvesting area, the algae liquid flows through the baffle (4) into the harvesting area, and the top of the harvesting area is the algae scraper (5), Behind the algae scraper (5) is the algae storage tank (6), the algae collection port (7) is located at the bottom of the algae storage tank (6), the slag discharge port (8) is located at the bottom of the harvesting area, and behind the harvesting area is the second baffle (9). The water outlet area is behind the plate two (9), and the clear water enters the water outlet area through the gap below the baffle plate two (9). 10), a butterfly valve (11) is installed on the water inlet pipe (10) of the dissolved air pump, and the clean water enters the air flotation system through the water inlet (10) of the dissolved air pump, and the water inlet pipe of the dissolved air pump is connected with the dissolved air inlet pipe (12), and then connected together To the dissolved air pump (13), the upper end of the dissolved air pump (13) is connected to the dissolved air tank (14), the lower end is connected to the dissolved air pump outlet pipe (16), and a dissolved air pressure gauge (15) is installed on the dissolved air pump outlet pipe (16) , the dissolved air pump liquid pipe (16) is then connected to the dissolved air release port (2).
本发明所述的一种电絮凝-气浮联用的微藻连续收获方法按以下步骤。 A method for continuously harvesting microalgae using electroflocculation-air flotation combined in the present invention comprises the following steps.
步骤1）待收藻液准备：从微藻光合反应系统中按一定流速导出藻液，同时按一定流速从苦卤水储罐中导出苦卤水，藻液与苦卤水混合物流经一个跑道池充分混合，进入在跑道池的末端的深槽（为待收藻液）；将藻液与苦卤水充分混合是为了提高藻液的电导率，降低收获所需能耗。 Step 1) Preparation of the algae solution to be collected: export the algae solution from the microalgae photosynthetic reaction system at a certain flow rate, and at the same time export the bittern from the bittern water storage tank at a certain flow rate, and the mixture of the algae solution and the bittern water flows through a raceway pool to fully mix , into the deep groove at the end of the runway pool (for the algae liquid to be harvested); the purpose of fully mixing the algae liquid with the bittern is to increase the conductivity of the algae liquid and reduce the energy consumption required for harvesting.
步骤2）微藻絮凝：待收藻类从藻液入口（1）泵入并上升到电解板（3），被电极释放出来的铁的氢氧化物胶体所絮凝。 Step 2) Microalgae flocculation: the algae to be harvested are pumped from the algae liquid inlet (1) and rise to the electrolytic plate (3), where they are flocculated by the iron hydroxide colloid released from the electrode.
步骤3）絮凝微藻气浮收获：被絮凝团聚的微藻细胞团，被溶气释放口（2）释放出来的微气泡所吸附上浮，微气泡上升过程中爆裂产生作用力促使微藻细胞团进一步团聚，微藻细胞团随主流流过挡板一（4）进入收获区，上升至液面，液体表面的微藻被刮藻机（5）刮入储藻池（6），所收集的藻浆通过储藻池（6）底部的收藻口（7）进行收集，送入下一步的脱水烘干工序。 Step 3) Flocculation microalgae air flotation harvesting: the microalgae cell clusters that are flocculated and reunited are adsorbed and floated by the microbubbles released from the dissolved air release port (2), and the bursting of the microbubbles in the process of rising produces force to promote the microalgae cell clusters Further agglomeration, the microalgae cell mass flows through the baffle 1 (4) along with the main flow into the harvesting area, rises to the liquid surface, and the microalgae on the liquid surface is scraped into the algae storage tank (6) by the algae scraper (5), and the collected algal pulp The algae is collected through the algae collection port (7) at the bottom of the algae storage tank (6), and sent to the next step of dehydration and drying process.
步骤4）清水排放：收获区底部的清水，通过挡板二（9）下部的空隙进入出水区，若液面较高则通过溢水口（17），排水管（18）将清水排放（清水排放速度与待收藻液流入速度平衡），大部分水通过溶气泵进水口（10）进入气浮系统并与溶气进气管（12）进气混合通过溶气泵（13）一部分混合液进入溶气罐（14）暂时储存，一部分通过溶气泵出液管（16）与溶气释放口（2）相连进入电絮凝气浮区，维持装置内的水位稳定。 Step 4) Clean water discharge: The clean water at the bottom of the harvesting area enters the water outlet area through the gap at the lower part of the baffle plate 2 (9). If the liquid level is high, the clean water is discharged through the overflow port (17) and the drain pipe (18) (clean water discharge The speed is balanced with the inflow speed of the algae liquid to be collected), most of the water enters the air flotation system through the water inlet of the dissolved air pump (10) and mixes with the air intake of the dissolved air inlet pipe (12) and enters the dissolved air through the dissolved air pump (13). The tank (14) is temporarily stored, and part of it is connected to the dissolved air release port (2) through the dissolved air pump outlet pipe (16) and enters the electrocoagulation air flotation area to maintain a stable water level in the device.
本发明的优点在于。 The advantage of the present invention is that.
（1）本发明创造性地将电絮凝与气浮分离两个工艺结合在一起，在同一装置内完成，省去了一些繁琐不必要的步骤，大大提高了微藻的收获效率。该装置投资少，占地面积小，操作简单，具有低能耗高效率，环保的优点，便于大规模生产。 (1) The present invention creatively combines the two processes of electrocoagulation and air flotation separation, and completes them in the same device, which saves some tedious and unnecessary steps and greatly improves the harvesting efficiency of microalgae. The device requires less investment, occupies a small area, is simple to operate, has the advantages of low energy consumption, high efficiency, and environmental protection, and is convenient for large-scale production.
（2）该装置电解板采用铁材，且电解板浸入液面下方钻孔，钻孔可以增加电板与水体的接触面积，提高电解效率。而且铁不像铝一样有毒性作用，铁价格也便宜仅仅是铝四分之一左右。 (2) The electrolytic plate of the device is made of iron, and the electrolytic plate is immersed in the bottom of the liquid surface to drill holes. The drilling can increase the contact area between the electric plate and the water body and improve the electrolysis efficiency. Moreover, iron is not as toxic as aluminum, and the price of iron is only about a quarter of that of aluminum.
（3）溶气气浮机的溶气释放口由多个小管口组成，使微气泡最大面积地覆盖电解板，其目的是保证溶气式气浮产生的微气泡充分与絮凝体接触，将其带到水体表面。 (3) The dissolved air release port of the dissolved air flotation machine is composed of multiple small nozzles, so that the microbubbles cover the electrolytic plate with the largest area. The purpose is to ensure that the microbubbles generated by the dissolved air flotation fully contact the flocs, and the It is brought to the surface of water bodies.
（4）可加入苦卤水提高电导率，电解产生的氢氧化镁絮凝体有较强的吸附能力。 (4) Bitter brine can be added to increase the conductivity, and the magnesium hydroxide flocs produced by electrolysis have strong adsorption capacity.
附图说明 Description of drawings
图1为本发明设备的结构示意图。 Fig. 1 is a structural schematic diagram of the device of the present invention.
图2为本发明设备的右视图。 Figure 2 is a right side view of the apparatus of the present invention.
图中：A为电絮凝气浮区，B为收获区，C为出水区，D为气浮系统。1为藻液入口，2为溶气释放口，3为电解板，4为挡板一，5为刮藻机，6为储藻池，7为收藻口，8为排渣口，9为挡板二，10为溶气泵进水管，11为蝶阀，12为溶气进气管，13为溶气泵，14为溶气罐，15为溶气压力表，16为溶气泵出液管，17为溢水口，18为排水管。 In the figure: A is the electrocoagulation air flotation area, B is the harvest area, C is the water outlet area, and D is the air flotation system. 1 is the algae liquid inlet, 2 is the dissolved air release port, 3 is the electrolytic plate, 4 is the baffle, 5 is the algae scraper, 6 is the algae storage pool, 7 is the algae collection port, 8 is the slag discharge port, and 9 is the baffle 2. 10 is the water inlet pipe of the dissolved air pump, 11 is the butterfly valve, 12 is the dissolved air inlet pipe, 13 is the dissolved air pump, 14 is the dissolved air tank, 15 is the dissolved air pressure gauge, 16 is the dissolved air pump outlet pipe, and 17 is the overflow port , 18 is drainpipe.
具体实施方式 detailed description
下面结合附图对本发明作进一步说明。 The present invention will be further described below in conjunction with accompanying drawing.
本发明所述的装置如附图1和附图2所示。 The device of the present invention is shown in accompanying drawing 1 and accompanying drawing 2.
本发明的微藻收获方法是从微藻光合反应系统中按一定按5L/MIN的流速导出藻液，同时按0.01L/MIN的流速从苦卤水储罐中导出苦卤水，藻液与苦卤水混合物流经一个跑道池充分混合，进入在跑道池的末端的深槽，是为待收藻液；启动本发明的电絮凝-气浮联用的微藻连续收获装置， 待收藻液通过藻液入口1按5.01L/MIN的流速进入电絮凝气浮区，待装置内的液面刚好没过电解板时，启动电絮凝与气浮装置，调节电絮凝装置电压微12伏特、电流强度为20安培、调节溶气泵进水管蝶阀11与溶气泵进气口阀门以及观察溶气泵进气速率表来控制进水量与溶气速率，使微气泡呈密集乳白状态，微藻絮凝漂浮在液面，并流经收获区，通过设备控制箱启动刮藻机，将藻的絮凝物刮入储藻池，再通过收藻口收获微藻，送入后续加工程序。收获区底部的清水，通过挡板二（9）下部的空隙进入出水区，若液面较高则通过溢水口（17），排水管（18）将清水排放，清水排放的流速控制为5.00L/MIN左右，使液面一直维持在刮藻机的工作区间。 The microalgae harvesting method of the present invention is to export the algae liquid from the microalgae photosynthetic reaction system at a certain flow rate of 5L/MIN, and simultaneously export the bittern from the bittern water storage tank at a flow rate of 0.01L/MIN, and the algae liquid and the bittern The mixture flows through a runway pool and is fully mixed, and enters the deep groove at the end of the runway pool, which is the algae liquid to be collected; start the electrocoagulation-air flotation combined microalgae continuous harvesting device of the present invention, and the algae liquid to be collected passes through the algae The liquid inlet 1 enters the electroflocculation air flotation area at a flow rate of 5.01L/MIN. When the liquid level in the device is just below the electrolytic plate, start the electroflocculation and air flotation device, adjust the voltage of the electroflocculation device to 12 volts, and the current intensity is 20 amps, adjust the butterfly valve 11 of the water inlet pipe of the dissolved air pump and the air inlet valve of the dissolved air pump, and observe the air intake rate meter of the dissolved air pump to control the water intake and dissolved air rate, so that the microbubbles are dense and milky, and the microalgae flocculate and float on the liquid surface. And flow through the harvesting area, start the algae scraper through the equipment control box, scrape the algae flocs into the algae storage tank, and then harvest the microalgae through the algae collection port, and send it to the subsequent processing procedure. The clear water at the bottom of the harvesting area enters the water outlet area through the gap at the lower part of the baffle plate 2 (9). If the liquid level is high, it passes through the overflow port (17), and the drain pipe (18) discharges the clear water. The flow rate of the clear water discharge is controlled to 5.00L /MIN to keep the liquid level in the working range of the algae scraper.
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| CN106399045B (en) * | 2015-07-31 | 2019-01-04 | 中国科学院青岛生物能源与过程研究所 | A kind of portable low energy consumption air-floating apparatus and application method for microalgae recovery |
| CN105176826B (en) * | 2015-10-20 | 2018-08-03 | 清华大学 | A kind of method and separator of alkalescent flocculation-foam fraction factor microalgae |
| CN107012072B (en) * | 2017-04-10 | 2023-10-31 | 南昌大学 | Optical biomembrane reactor and application thereof in sewage treatment, carbon fixation and microalgae collection |
| CN107827211B (en) * | 2017-12-13 | 2023-07-18 | 重庆大学 | An electrochemical reaction tank for treating algae-containing water |
| CN115678772A (en) * | 2022-11-17 | 2023-02-03 | 常州大学 | A comprehensive utilization device for algae removal |
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| CN101811757A (en) * | 2010-04-26 | 2010-08-25 | 中国科学院过程工程研究所 | Air-assisted electro-coagulation algae water separation device and using method thereof |
| CN202519115U (en) * | 2012-04-19 | 2012-11-07 | 波鹰(厦门)科技有限公司 | Nano-catalysis, electrolysis, flocculation and air-floatation device |
| CN204848533U (en) * | 2015-04-13 | 2015-12-09 | 南昌大学 | Electricity flocculation - air supporting allies oneself with continuous harvesting apparatus of little algae of usefulness |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101811757A (en) * | 2010-04-26 | 2010-08-25 | 中国科学院过程工程研究所 | Air-assisted electro-coagulation algae water separation device and using method thereof |
| CN202519115U (en) * | 2012-04-19 | 2012-11-07 | 波鹰(厦门)科技有限公司 | Nano-catalysis, electrolysis, flocculation and air-floatation device |
| CN204848533U (en) * | 2015-04-13 | 2015-12-09 | 南昌大学 | Electricity flocculation - air supporting allies oneself with continuous harvesting apparatus of little algae of usefulness |
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