CN104800135B - It is a kind of to have light spot, moisturizing, sun-proof Chinese medicine cosmetic concurrently - Google Patents

Abstract

The present invention relates to the traditional Chinese medicine cosmetics with anti-spot, moisturizing and sun protection, which can effectively solve the problem of cosmetics with anti-spot, moisturizing and sun protection. ‑10%, asparagus extract 0.1‑1%, safflower extract 5‑10%, cockscomb extract 5‑20%, ascorbic acid 2‑3%, olive oil 12‑17%, isopropyl palmitate4 -6%, polyoxyethylene nonylphenol ether 0.4-0.6%, glycerin 4-6%, methyl p-hydroxybenzoate 0.08-0.12%, ethanol 6-8%, and the balance is deionized water, the prescription of the present invention Scientific and reasonable, it has the functions of invigorating qi and nourishing yin, promoting blood circulation and removing blood stasis, moisturizing and whitening, strengthening sunscreen, effectively removing free radicals, inhibiting the activity of tyrosinase, strengthening skin metabolism, reducing the damage of ultraviolet rays to the skin, natural and non-irritating, It is a great innovation in cosmetics to achieve the effect of comprehensive whitening of the skin, and it has outstanding economic and social benefits.

Description

A traditional Chinese medicine cosmetic with functions of lightening spots, moisturizing and sun protection

technical field

The invention relates to medicine, in particular to a traditional Chinese medicine cosmetic with the functions of lightening spots, moisturizing and sun protection.

Background technique

Everyone has the desire for beauty, especially young women nowadays pursue "white and rich beauty", and everyone hopes to have healthy and fair skin. There are two ways for the source of pigment: one is to activate tyrosinase to produce dopaquinone, form dopa pigment, and finally form melanin; the other way is to generate active oxygen and lipid peroxide in the skin, and lipid peroxide makes collagen Cross-linking, in addition to making the skin lose its elasticity, can also be decomposed into malondialdehyde and other substances under the action of oxidase. These substances react with proteins to form brown pigment spots and darken the skin color. The latter pathway does not require the participation of tyrosinase and is an inevitable free radical damage process in the cell body.

In order to prevent pigmentation, the basic principles of whitening cosmetics are mainly reflected in the following aspects: 1. Inhibit the production of melanin. Prevents melanin production by inhibiting tyrosinase activity, or interfering with melanin-producing intermediates. 2. Reduction of pigments and prevention of photooxidation. Stimulates the reduction of melanin through keratinocytes to lighten the already produced melanin. 3. In the metabolic process of the skin, reduce pigmentation. By improving the metabolism of the skin, the melanin can be quickly discharged out of the skin. 4. Prevent the entry of ultraviolet rays. Through preparations with sunscreen effect, physical methods are used to block ultraviolet rays and reduce excessive melanin caused by ultraviolet rays.

Based on the formation mechanism of melanin, the main pathway of whitening cosmetics is to resist ultraviolet rays, hinder the activity of tyrosinase, change the production pathway of melanin, and scavenge free radicals to reduce and decolorize melanin. Therefore, the development of all-round whitening cosmetics has become the mainstream of the current whitening market.

The skin is a large-area organ exposed to the outside of the human body, and the water in the cells and matrix is easily lost. There are no blood vessels in the epidermis of the skin, and the source of moisture can only be supplied by the penetration of the dermis. Therefore, the epidermis is the most vulnerable part of the body to lack water. The lack of water will affect the division and secretion ability of skin cells, reduce the metabolism of pigment, and reduce the moisturizing effect on itself. Effectively replenishing and maintaining the skin's moisture is an important aspect that cannot be ignored in reducing the appearance of pigment accumulation in the skin. Therefore, keeping the skin moisturized is the basis for freckle removal and whitening.

The current whitening products mainly inhibit the activity of tyrosinase. Although there are many kinds of cosmetics used for health care, due to various reasons, their use is not satisfactory and cannot meet the practical application. Therefore, the improvement of cosmetics and innovation is imperative.

Contents of the invention

In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a Chinese medicine cosmetic with lightening, moisturizing and sunscreen, which can effectively solve the problem of cosmetics with lightening, moisturizing and sunscreen.

The technical solution solved by the present invention is that the cosmetic is made by mixing the following raw materials in percentage by weight: 5-10% of ginseng extract, 0.1-1% of asparagus extract, 5-10% of safflower extract, cockscomb Flower extract 5-20%, ascorbic acid (vitamin C) 2-3%, olive oil 12-17%, isopropyl palmitate 4-6%, polyoxyethylene nonylphenol ether 0.4-0.6%, glycerin 4- 6%, 0.08-0.12% methyl p-hydroxybenzoate, 6-8% ethanol, and the balance is deionized water (that is, add deionized water to 100%), wherein the ginseng extract is, the quality of ginseng Ethanol with a concentration of 50% is used as a solvent. The amount of solvent used is 6-8 times the weight of ginseng. Heating and reflux extracting twice, one hour at a time, combining the two ethanol extracts, recovering ethanol, and concentrating to an extract with a relative density of 1.2-1.4 , freeze at -80°C for 24 hours, and vacuum-dry to obtain a freeze-dried powder, namely the adult ginseng extract;

The described asparagus extract is that asparagus uses ethanol with a mass concentration of 70% as a solvent, and the amount of solvent is 6-8 times the weight of asparagus, heated and refluxed for 2 extractions, one hour at a time, and the two ethanol extracts are combined , recovering ethanol, concentrating to an extract with a relative density of 1.2-1.4, freezing at -80°C for 24 hours, and vacuum-drying to obtain a lyophilized powder, which is the asparagus extract;

The described safflower extract is that safflower uses ethanol with a mass concentration of 50% as a solvent, and the amount of solvent used is 6-8 times the weight of safflower, heated and refluxed for 2 extractions, one hour at a time, and the two ethanol extracts are combined , recovering ethanol, concentrating to an extract with a relative density of 1.2-1.4, freezing at -80°C for 24 hours, and drying in a vacuum to obtain a freeze-dried powder, which is the safflower extract;

The extract of Celosia celosia is that Celosia celosia uses 90% ethanol as a solvent, and the amount of solvent is 6-8 times of the weight of Celosia celosia, and it is heated and refluxed for 2 extractions, one hour at a time, and the ethanol extracts are combined twice, Recover ethanol, concentrate to the extract with a relative density of 1.2-1.4, freeze at -80°C for 24 hours, and vacuum-dry to obtain a freeze-dried powder, which is the cockscomb extract.

The composition of the invention is scientific and reasonable, has the functions of invigorating qi and nourishing yin, promoting blood circulation and removing blood stasis, moisturizing and whitening, strengthening sun protection, can effectively remove free radicals, inhibit the activity of tyrosinase, strengthen skin metabolism, and reduce the damage of ultraviolet rays to the skin. Natural and non-irritating, it achieves the effect of comprehensive whitening of the skin. It is a major innovation in cosmetics and has outstanding economic and social benefits.

detailed description

The specific implementation of the present invention will be described below in conjunction with the examples.

The present invention can be provided by the following examples in concrete implementation.

Example 1

In the specific implementation of the present invention, the following raw materials are uniformly mixed in weight percentage: 7-8% of ginseng extract, 0.4-0.6% of asparagus extract, 7-8% of safflower extract, 10% of cockscomb extract -15%, ascorbic acid 2.3-2.6%, olive oil 13-16%, isopropyl palmitate 4.5-5.5%, polyoxyethylene nonylphenol ether 0.45-0.55%, glycerin 4.5-5.5%, methyl p-hydroxybenzoate Ester 0.09-0.11%, ethanol 6.5-7.5%, and the balance is deionized water.

Example 2

In the specific implementation of the present invention, it can also be prepared by mixing the following raw materials in percentage by weight: 5% ginseng extract, 0.5% asparagus extract, 5% safflower extract, 8% cockscomb extract, and 2% ascorbic acid , olive oil 15%, isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerin 5%, methyl paraben 0.1%, ethanol 7%, add deionized water to 100%.

Example 3

In the specific implementation of the present invention, it can also be prepared by mixing the following raw materials in percentage by weight: 9% of ginseng extract, 0.7% of asparagus extract, 7% of safflower extract, 15% of cockscomb extract, and 2% of ascorbic acid , olive oil 15%, isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerin 4%, methyl paraben 0.1%, ethanol 8%, add deionized water to 100%.

Example 4

In the specific implementation of the present invention, it is prepared by mixing the following raw materials in percentage by weight: 10% of ginseng extract, 1% of asparagus extract, 10% of safflower extract, 18% of cockscomb extract, ascorbic acid (vitamin C ) 2.5%, olive oil 16%, isopropyl palmitate 6%, polyoxyethylene nonylphenol ether 0.4%, glycerin 6%, methyl p-hydroxybenzoate 0.11%, ethanol 8%, add deionized water to 100 %.

Among the components of the present invention, wherein:

Celosia uses extracts from its dried flowers, which are rich in nutrients such as amino acids, trace elements, and natural enzymes, which can be used as antioxidants, skin whitening agents, moisturizers, etc. Celosia is rich in nutrients, such as protein, fat, folic acid, pantothenic acid, vitamin B1 and other vitamins, 21 kinds of amino acids, 13 kinds of trace elements and more than 50 kinds of natural enzymes. Contains bioactive ingredients of flavonoids and some red pigments.

Safflower is a herbaceous plant of the genus Safflower in the Compositae family. This product uses its dried flowers, which contain flavonoids, phenolic acids and linoleic acid. Safflower extract has the effects of promoting blood circulation, anti-inflammation, and anti-oxidation. By improving skin blood circulation, promoting skin metabolism, scavenging free radicals, inhibiting melanin deposition, accelerating spot depigmentation, and absorbing ultraviolet rays, it exerts the effects of whitening, sun protection, and anti-aging .

Ginseng mainly uses its dried root extract, which mainly contains more than 40 kinds of ginsenoside monomers, and also contains ginseng polysaccharides and other alkaloids such as ginseng choline. Ginseng extract can be used as skin whitening agent, anti-inflammatory agent, moisturizing agent and sunscreen agent.

Asparagus, also known as asparagus, this product uses the extract of its dried root, the main components are saponins and amino acids. The extract can be used as antioxidant, skin aging preventive agent, whitening agent and moisturizing agent. The extract also has antibacterial effect, and has different degrees of antibacterial effect on Staphylococcus aureus, Staphylococcus citrinus, Staphylococcus albus and Bacillus subtilis, which is beneficial to the storage of cosmetics. The above-mentioned four kinds of Chinese medicine composition distribution ratios of the present invention are

The ginseng of the present invention has the good reputation of "the king of all herbs" since ancient times, and is also praised as the ultimate product of "nourishing yin and nourishing health, strengthening the body and strengthening the body" by the oriental medicine circle. Asparagus has the effects of nourishing yin and clearing away heat, nourishing the lungs and nourishing the kidneys. Safflower contains flavonoids, phenols and fatty acids, etc. It is known as the "king of linoleic acid" and has the effect of promoting blood circulation and removing blood stasis. Celosia is rich in nutrients, trace elements, natural enzymes, bioactive components of flavonoids and some red pigments.

According to the overall concept of traditional Chinese medicine, the skin is a part of the human body, and the lungs govern the fur, which is a delicate organ. If the lungs are deficient in qi and yin, the skin is prone to dryness and chlorosis; . Ginseng invigorates qi, asparagus nourishes yin, safflower and cockscomb promotes blood circulation and removes blood stasis. The above traditional Chinese medicine ingredients cooperate with other components to synergize, invigorate qi and nourish yin, promote blood circulation and remove blood stasis, moisturize and whiten, strengthen the body and sunscreen, It can effectively scavenge free radicals, inhibit the activity of tyrosinase, strengthen skin metabolism, reduce the damage of ultraviolet rays to the skin, natural and non-irritating, and achieve the effect of comprehensive whitening of the skin. And achieved very satisfactory beneficial technical effects through tests, the relevant test data are as follows:

1. Inhibition of melanocyte division

The melanin synthesis function of mouse B16 melanocytes is basically the same as that of normal human skin melanocytes. Melanocytes are seeded in 96-well plates at 1×10 ⁴ cell/well, 100ul per well, and placed in a 37°C, 5% CO ₂ incubator Cultivate in medium for 2 days; absorb the original culture medium and add the complete culture medium respectively. The four kinds of traditional Chinese medicine extracts to be tested were mixed in proportion and diluted into 5 solutions with different final concentrations of 500 mg/L, 250 mg/L, 125 mg/L, 62.5 mg/L, and 31.25 mg/L. For 5 parallel wells, no drug was added to the control group, and the same amount of complete medium was added, and the culture was continued for 3 days in the incubator. Remove the culture supernatant, add 100 μL of 0.5 mg.L ^-1 MTT to each well, continue to incubate for 4 h, and add 150 μL DMSO to dissolve the purple precipitate, and measure the absorbance (A) at a wavelength of 570 nm (/630 nm) with a microplate reader. The absorbance value of other wells is divided by the average absorbance value of normal wells, multiplied by 100%, as the survival rate of each well, inhibition rate=1-survival rate; according to the inhibition rate curve, calculate the lethal concentration range of the test substance and The IC50 median lethal concentration is the toxicity index of the whitening ingredients of the traditional Chinese medicine (see Table 1);

Table 1 Inhibitory rate of melanocytes

Drug concentration (mg/L) 500 250 125 62.5 31.25 control Inhibition rate% 64.10±8.32 ^▲ 49.66±7.02 ^▲ 31.16±4.82 ^▲ 17.54±3.45 ^▲ 11.91±2.05 ^▲ 4.13±0.84

Note: Compared with the control group: ▲P﹤0.05

As the concentration of Chinese medicine whitening ingredients increases, the inhibition rate of melanocytes also increases correspondingly, and the toxicity index is 250mg/ml, which means that the concentration of Chinese medicine whitening ingredients in whitening skin creams cannot exceed this concentration.

2 Inhibition of tyrosinase activity

After 3 days of treatment with traditional Chinese medicine whitening ingredients (see the concentration above), the supernatant was discarded, washed twice with pH 6.8 PBS, and 90uL of PBS containing 1% (volume fraction) Triton X-100 was added to each well. Sonicate in an ice bath, add 10uL 1×10-2mol/L L-dopa to each well, incubate at 37°C for 60min, use a UV spectrophotometer to measure color at 490nm, set the blank well to zero, and measure the absorbance of each well (see Table II). Inhibition rate of tyrosinase activity=(1-average absorbance value of each concentration/average absorbance value of control group)*100%

Table 2 Inhibition rate of tyrosinase activity

Drug concentration (mg/L) 500 250 125 62.5 31.25 Inhibition rate% 37.98 32.44 26.34 19.26 5.94

It can be seen from Table 2 that as the concentration of traditional Chinese medicine whitening ingredients decreases, the inhibitory effect on tyrosinase activity also decreases, that is to say, as the drug concentration increases, its inhibitory effect also increases. Inhibition of tyrosinase activity.

3 The lightening effect of whitening skin cream on pigment

Choose 15 male and female volunteers with darker skin. After cleansing in the morning and evening, apply the skin cream containing traditional Chinese medicine whitening ingredients on the right face at intervals of 12 hours. Sensory Evaluation Fill out the trial evaluation form. The highest score is 4, the effect is significant, and the lowest score is 1, the contrast effect is not obvious. In the first week (Table 3), the second week (Table 4), the third week (Table 5), and the fourth week (Table 6) the chi-square was used for statistics.

Table 3 Evaluation of the effect after the first week of application

Evaluation effect 1 2 3 4 left face 15 0 0 0 right face 13 1 1 0

No difference

Table 4 Evaluation of the effect after the second week of application

Evaluation effect 1 2 3 4 left face 14 1 0 0 right face 3 11 1 0

P﹤0.05

Table 5 Evaluation of the effect after the third week of application

Evaluation effect 1 2 3 4 left face 14 1 0 0 right face 1 4 10 0

P﹤0.05

Table 6 Evaluation of the effect after the fourth week of application

Evaluation effect 1 2 3 4 left face 13 2 0 0 right face 0 4 2 9

P﹤0.05

After smearing for a week for comparison and observation, there is no obvious contrast between the left and right sides of the face, but as time goes on, after two, three, and four weeks of use, the right face has an obvious effect of lightening spots (P﹤0.05).

4 The sun protection effect of whitening skin cream

Take 5 rats (body weight 400g-450g), carefully cut off the symmetrical parts of 3cm×3cm on both sides of the back after ether anesthesia, put them in a mouse cage, and place them under an ultraviolet sterilizing lamp (25W 1 stick, distance 100cm), apply an appropriate amount of whitening skin cream evenly on the skin on the right side of the back, and only apply paste without traditional Chinese medicine ingredients on the left side, and irradiate it under an ultraviolet lamp for 8 hours every day (repeat the anesthesia and apply sunscreen once in 12 hours), Take it out and continue to raise it for 7 days, then kill it, wipe the skin surface with a cotton ball alternately dipped in water and ethanol to remove the residual cream, and measure its thickness with a vernier.

Table 7. Rat left and right side skin thickness measurement table (unit: um)

serial number 1 2 3 4 5 left side 48.6 42.9 45.6 50.1 47.8 Right 41.9 38.2 44.0 40.1 39.7

Ultraviolet damage can increase the thickness of epidermal cells, not only destroy the outermost skin cells, but also reach the dermal cells through the epidermis, resulting in massive necrosis and metabolic disorders of dermal cells, impurity in cells cannot be metabolized, loss of elasticity, premature aging, and sensitivity. Wrinkles appear and thickness increases. It can be seen from the data in the above table that the skin on the right side is thinner than that on the left side, and the skin on the right side of the rat’s back has been protected to a certain extent after being applied with whitening skin cream.

5 Moisturizing test of whitening skin cream

Using the in vitro weighing method (refer to the weighing method used in Dermatology and Cosmetic Efficacy Evaluation edited by Liu Wei and Zhang Huailiang), at a constant temperature of 20°C, 5g of whitening skin cream and 5g of matrix were evenly applied to the two cultures. Put it on the bottle cap of the dish, let it stand for 8 hours, weigh the water reduction every 1 hour, and calculate the water retention: residual water mass/sample mass.

Table 8 Water retention test of whitening skin cream for 8 hours

time interval (h) 1 2 3 4 5 6 7 8 Water retention (%) 98.24 90.84 89.34 87.12 86.51 79.24 60.46 57.12

It can be seen from Table 8 that with the prolongation of time, the amount of residual water decreases to a certain extent. After 8 hours, the water retention can be maintained at more than half, which has a good moisturizing effect.

In a word, it can be clearly proved from the above tests that the present invention has the effects of lightening spots, keeping warm, and preventing sun, and is effectively used for human skin protection and health care. It is a great innovation in cosmetics, has strong practical value, and has significant economic benefits. and social benefits.

Claims (5)

1. a kind of have light spot, moisturizing, sun-proof Chinese medicine cosmetic concurrently, it is characterised in that by the raw material of following percentage by weight meter It is well mixed to be made：Ginseng extract 5-10%, Radix Asparagi extract 0.1-1%, safflower extract 5-10%, cockscomb flower extract 5- 20%th, ascorbic acid 2-3%, olive oil 12-17%, isopropyl palmitate 4-6%, polyoxyethylene nonylphenol ether 0.4-0.6%, glycerine 4-6%, methyl p-hydroxybenzoate 0.08-0.12%, ethanol 6-8%, surplus is deionized water, wherein, described ginseng extract It is that ginseng is solvent with the ethanol of mass concentration 50%, the consumption of solvent is 6-8 times of ginseng weight, heating and refluxing extraction 2 times, One time one hour, merge ethanol extract twice, reclaim ethanol, be concentrated into relative density 1.2-1.4 medicinal extract, -80 DEG C of freezings 24 hours, be dried in vacuo to obtain freeze-dried powder, ginseng extract；

Described Radix Asparagi extract is that asparagus fern is solvent with the ethanol of mass concentration 70%, and the consumption of solvent is the 6- of asparagus fern weight 8 times, heating and refluxing extraction 2 times one time one hour, merges ethanol extract twice, reclaims ethanol, be concentrated into relative density 1.2- 1.4 medicinal extract, -80 DEG C freeze 24 hours, be dried in vacuo to obtain freeze-dried powder, Radix Asparagi extract；

Described safflower extract is that safflower is solvent with the ethanol of mass concentration 50%, and the consumption of solvent is the 6- of safflower weight 8 times, heating and refluxing extraction 2 times one time one hour, merges ethanol extract twice, reclaims ethanol, be concentrated into relative density 1.2- 1.4 medicinal extract, -80 DEG C freeze 24 hours, be dried in vacuo to obtain freeze-dried powder, safflower extract；

Described cockscomb flower extract is that cockscomb is solvent with the ethanol of mass concentration 90%, and the consumption of solvent is cockscomb weight 6-8 times, heating and refluxing extraction 2 times one time one hour, merges ethanol extract twice, reclaims ethanol, be concentrated into relative density 1.2-1.4 medicinal extract, -80 DEG C freeze 24 hours, be dried in vacuo to obtain freeze-dried powder, cockscomb flower extract.

2. according to claim 1 have light spot, moisturizing, sun-proof Chinese medicine cosmetic concurrently, it is characterised in that by following weight The raw material of percentages is well mixed to be made：Ginseng extract 7-8%, Radix Asparagi extract 0.4-0.6%, safflower extract 7-8%, Cockscomb flower extract 10-15%, ascorbic acid 2.3-2.6%, olive oil 13-16%, isopropyl palmitate 4.5-5.5%, polyoxy second Alkene nonyl phenolic ether 0.45-0.55%, glycerine 4.5-5.5%, methyl p-hydroxybenzoate 0.09-0.11%, ethanol 6.5-7.5%, it is remaining Measure as deionized water.

3. according to claim 1 have light spot, moisturizing, sun-proof Chinese medicine cosmetic concurrently, it is characterised in that by following weight The raw material of percentages is well mixed to be made：Ginseng extract 5%, Radix Asparagi extract 0.5%, safflower extract 5%, cockscomb are carried Take thing 8%, ascorbic acid 2%, olive oil 15%, isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerine 5%, to hydroxyl Methyl benzoate 0.1%, ethanol 7%, plus deionized water is to 100%.

4. according to claim 1 have light spot, moisturizing, sun-proof Chinese medicine cosmetic concurrently, it is characterised in that by following weight The raw material of percentages is well mixed to be made：Ginseng extract 9%, Radix Asparagi extract 0.7%, safflower extract 7%, cockscomb are carried Take thing 15%, ascorbic acid 2%, olive oil 15%, isopropyl palmitate 5%, polyoxyethylene nonylphenol ether 0.5%, glycerine 4%, to hydroxyl Yl benzoic acid methyl esters 0.1%, ethanol 8%, plus deionized water is to 100%.

5. according to claim 1 have light spot, moisturizing, sun-proof Chinese medicine cosmetic concurrently, it is characterised in that by following weight The raw material of percentages is well mixed to be made：Ginseng extract 10%, Radix Asparagi extract 1%, safflower extract 10%, cockscomb are carried Take thing 18%, it is ascorbic acid 2.5%, olive oil 16%, isopropyl palmitate 6%, polyoxyethylene nonylphenol ether 0.4%, glycerine 6%, right Methyl hydroxybenzoate 0.11%, ethanol 8%, plus deionized water is to 100%.