CN104569300B - The detection means of analyte in a kind of liquid sample - Google Patents
The detection means of analyte in a kind of liquid sample Download PDFInfo
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- CN104569300B CN104569300B CN201310502781.XA CN201310502781A CN104569300B CN 104569300 B CN104569300 B CN 104569300B CN 201310502781 A CN201310502781 A CN 201310502781A CN 104569300 B CN104569300 B CN 104569300B
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- 238000002347 injection Methods 0.000 claims abstract description 89
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- 238000000034 method Methods 0.000 claims description 18
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- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of detection means of analyte in liquid sample, including：Test chamber for accommodating testing element；Injection port；One gas allowed in test chamber carries out the equipment that gas is exchanged with the gas outside test chamber by injection port.Complete the test of analyte in liquid sample automatically into test chamber by liquid sample by gas differential pressure.
Description
Technical field
The present invention relates to a kind of detection means, especially a kind of detection for detecting illegal drug in liquid sample.
Background technology
At present, general public obtains that illicit drug is more and more easier, therefore the use of illicit drug is also more and more.In order to ensure
The safety of working environment and the fair competition of physical culture, enterprise, unit and sports organization need to detect the body fluid example of employee, sportsman
As urinated, to understand whether employee and sportsman have used illicit drug.Therefore Humoral sample Detection and Collect device is more and more,
And need to be operated by layman in common occasion.Using traditional liquid sample collection detection means, there is sample appearance
Easily because the inaccurate of sample size causes " Flooding " phenomenon or undetectable situation in leakage and detection, it is impossible to full
The safety detection of sufficient liquid sample and the requirement of Accurate Determining.
The similar device in existing technology, in US5,119,830, detection means is arranged at the cup lid of urine cup
On.When the cup lid for opening urine cup, cup body is available for the collection and storage of sample.When needing detection, sample is first gathered, then
The part of a projection on cup lid is pressed, the barrier film between detection means and cup body is poked, cup body is then inverted a timing
Between, sample can enter the absorbent section of detection means from cup body, so as to be detected along the breach crushed by projection.
US5, discloses another similar detection means in 595,187, detection means is similarly disposed on cup lid.Collect
, it is necessary to which when detecting, a valve on pressing cup lid makes temporary liquid enter a cavity, will after certain time after sample
Cup body is turned, and the liquid sample decontroled in valve, cavity can be contacted with detection means, carries out detection reaction.
In addition, a utilization filter paper water suction is disclosed in published application US2004/0081581 directs fluid to detection
Detection means on reagent strip.The device contacts sample solution and filter paper pads, sample is guided by filter paper pads by overturning cup body
This further contacts the absorbent section of reagent strip.Such device is typically not in sample Overdosing problems, but there is sample
This deficiency causes the defect that detection fails.
In addition, disclosing a kind of urine cup of the edge-on detection of transverse direction in United States Patent (USP) US6,726,879B2, the urine cup includes
Three chambers, one is collecting chamber, and one is liquid transfer chamber, and one is test chamber, and two cup lids, and one of cup lid is used
In the sealing of collecting chamber, another cup lid is used for detecting in sample whether contain analyte.Introduce and pass through in the patent
The direct contact liq sample in sample application zone of detection reagent bar upstream, realizes testing goal.
This can be not only determined the liquid sample that needs are detected it is still necessary to provide a kind of new detection means
Sample is measured, while convenient operation, improves the accuracy of detection.
The content of the invention
On the one hand, the present invention provides such a detection means, including：Test chamber for accommodating testing element；Sample introduction
Mouthful；One gas allowed in test chamber carries out the equipment that gas is exchanged with the gas outside test chamber by injection port, so as to allow inspection
The liquid sample surveyed outside chamber is entered in test chamber by described injection port.
It is preferred that, the space outside the test chamber includes the collecting chamber for being used to collect fluid sample.It is preferred that, described liquid
Body sample is located in collecting chamber.
On the one hand, the present invention provides such a detection means, including：Test chamber for accommodating testing element；Sample introduction
Mouthful；One gas allowed in test chamber carries out what gas was exchanged with the gas in the collecting chamber outside test chamber by injection port
Equipment, so that the liquid sample allowed in collection chamber is entered in test chamber by described injection port.
On the one hand, the present invention provides detection means, including：Test chamber for accommodating testing element；Injection port；Liquid-like
This collecting chamber；The gas of one gas that allows in test chamber and collecting chamber carries out the equipment that gas is exchanged by injection port, so that
The liquid sample allowed in collecting chamber is entered in test chamber by described injection port.
On the one hand, the present invention provides detection means, including：Liquid sample collection chamber；One is used to seal collecting chamber opening
Lid, the lid includes being used to accommodate the test chamber of testing element；Injection port；One gas and collecting chamber that allow in test chamber
Gas by injection port carry out gas exchange equipment so that the liquid sample allowed in collecting chamber is entered by described injection port
Enter into test chamber.
In above all of embodiment, air pressure and the air pressure outside test chamber that the equipment of gas exchange allows in test chamber
Difference is produced, the liquid sample that the air pressure of the difference makes way for injection port enters in test chamber.
In some preferred modes, described gas alternating current equipment includes the cavity of a receiving gas, the receiving gas
The volume of the cavity of body for example can first diminish or can first increase to be transformable, or first diminish, rear to become big, and inspection is allowed so as to realize
The gas for surveying intracavitary passes through gas exchanges outside injection port and test chamber.
In some preferred modes, air pressure hair of the cavity of gas before occurring Volume Changes or in test chamber is being accommodated
Before changing, injection port is detected the liquid sample sealing outside chamber.In some preferred modes, part accommodates the cavity of gas
Including elastic flexible material, by the deformation of elastic flexible material, the volume for the cavity for accommodating gas is allowed to change.At some
In specific mode, described elastic flexible material is self elastically-deformable material, and deformation of elastic element is allowed by external force, from
And the gas allowed in test chamber is rejected to outside test chamber from injection port, after external force disappearance or partial disappearance, flexible member is replied
To original state, so that the liquid sample allowed in collecting chamber is entered in test chamber by injection port.
It is preferred that, deformation of elastic element is allowed by external force, so that the gas allowed in test chamber is rejected to collection from injection port
In chamber.
In preferred mode, the cavity of the receiving gas and test chamber gas connection, or accommodate the cavity of gas with
The air pressure of test chamber is the same or identical.It is preferred that, the cavity for accommodating gas passes through passage and test chamber gas connection.
In some preferred modes, being accommodated in test chamber can be with the test member of analyte in test liquid samples
Part.The liquid sample entered by injection port in test chamber being capable of engaged test element.It is preferred that, into the liquid-like of test chamber
The sample applying zone of sheet engaged test element first.In some modes, testing element includes water-absorbing material, liquid sample
It can be flowed by capillary force on testing element.
In some preferred modes, the equipment of gas exchange includes automatic air-exchanging motor, is allowed by automatic air-exchanging motor
Gas in test chamber is reduced, so as to produce negative pressure.
In some preferred modes, described injection port is close to the sample reception region of testing element.It is excellent at other
In the mode of choosing, the injection port two ends for being arranged on test chamber relative with gas alternating current equipment.It is preferred that, gas alternating current equipment quilt
It is positioned close to the suction zone of testing element.
On the other hand, the present invention provides a kind of method for detecting analyte in liquid sample, and this method includes：There is provided
Detection means, the detection means includes：Test chamber for accommodating testing element；Injection port；One gas that allows in test chamber
The air chamber that gas is exchanged is carried out by injection port with the gas outside test chamber, so that the liquid sample allowed outside test chamber passes through institute
The injection port stated is entered in test chamber；Liquid sample is allowed to contact injection port, so as to seal injection port；The volume of air chamber is allowed to subtract
Few, the excessive gas made way in test chamber is rejected to outside test chamber by injection port；The volume of air chamber is allowed to increase again,
The volume of increase allows test chamber is interior to form negative pressure, and the negative pressure of the formation forces the liquid sample at injection port to enter detection
Chamber.
In some preferred modes, the volume of described air chamber can change, and allow the air pressure of test chamber also to send out
Changing.In other preferred modes, the change that the air pressure of test chamber occurs forces the liquid sample positioned at injection port to enter
Enter in test chamber.In some preferred modes, it is preferred that the cavity that part accommodates gas includes elastic flexible material, passes through
The deformation of elastic flexible material, allows the volume for the cavity for accommodating gas to change.In some specific modes, described bullet
Property flexible material be self elastically-deformable material, deformation of elastic element is allowed by external force, thus the gas allowed in test chamber from
Injection port is rejected to outside test chamber, and when external force disappears or after partial disappearance, flexible member is returned to original state, so as to allow collection
The liquid sample of intracavitary is entered in test chamber by injection port.
It is preferred that, allow deformation of elastic element to reduce the volume of air chamber by external force, thus the gas allowed in test chamber from
Injection port is rejected in collecting chamber.
In above all of embodiment, before the air pressure difference is produced, the described injection port of liquid sample sealing.
Beneficial effect
Pass through the detection means of the present invention, it is possible to achieve the automatic acquisition of liquid sample, automatic detection simultaneously reaches that sampling is fixed
The purpose of amount.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram of one embodiment of the invention；
Fig. 2 is the assembling dimensional structure diagram of another embodiment of the present invention；
Fig. 3 is the operating process structural profile schematic diagram of one embodiment of the invention（Device is tilted）；
Fig. 4 is the operating process schematic diagram of one embodiment of the invention（The cavity volume for accommodating gas diminishes）；
Fig. 5 is the operating process schematic diagram of one embodiment of the invention（The cavity volume for accommodating gas increases again）；
Fig. 6 is the cross-sectional view of another embodiment of the present invention；
Description of reference numerals：
Urine cup body 200；Collecting chamber 202；The opening 201 of collecting chamber；Collecting chamber bottom 203；The projection 204 of collecting chamber bottom；
Cup lid 100, upper lid 101, testing element 102;Upper lid chamber 109；Gas held cavity 108；The top 116 of gas held cavity；
The side wall 135 of gas held cavity；Air chamber 113；Firm banking 107；Lower cover 104；Neck 103；Injection port 120；Gas leads to
Road 114；Test chamber 115；Sample reception region 117；Detection zone 118；Absorption region 119；Lower cover cavity 105；Tee-slot
140；Liquid sample 300；Sealing ring 110；Pipe 150.
Embodiment
The structure or these used technical terms that are related to the present invention are described further below.
Detection
Detection represents chemical examination or tests a kind of material or material whether there is, such as, but is not limited to this, chemical substance,
The generation of organic compound, inorganic compound, metabolism product, medicine or drug metabolite, organic organization or organic organization
Thank thing, nucleic acid, protein or polymer.In addition, detection represents test substances or the quantity of material.Furtherly, table is gone back in chemical examination
Show immune detection, chemical detection, enzyme detection etc..
Sample
Signified sample of the invention, which refers to those, whether there is analyte interested for detection, chemical examination or diagnosis
The material of matter.Sample can be, for example, liquid sample, liquid sample can include blood, blood plasma, serum, urine, saliva and
Various juices, can also include the liquid solution that solid sample and semi-solid samples are formed after anticipating.Collect and
Sample can be used for the methods such as immune detection, chemical detection, enzyme detection and detect whether to have analyte.
Analyte
Any analyte can be analyzed with apparatus and method of the present invention.Analyte can in any liquid or
Detected in person's liquefied sample, for example urine, saliva, saliva, blood, blood plasma, or serum.
Analyte can also be some haptens materials, and these haptens include drugs（Such as drug abuse）." abuse
Medicine " (DOA) refers to that non-medical destination uses medicine（Generally play paralysis nerve）.It can be used for using the device
Detection belongs to medical usage but the easily detection of overdose, such as tricyclic antidepressant（Imipramine or the like）And acetyl
Amino phenols etc..These medicines can resolve into different small-molecule substances after being absorbed by the body, and these small-molecule substances are present in blood
In the body fluid such as liquid, urine, saliva, sweat or part body fluid has above-mentioned small-molecule substance.
Detection means
In a specific embodiment, the detection means that the present invention is provided includes：Inspection for accommodating testing element
The air chamber of chamber, injection port and variable volume is surveyed, the gas allowed by the stereomutation of air chamber in test chamber passes through injection port
Swapped with extraneous liquid sample.In a preferred mode, the air chamber of variable volume is by elastic flexible material group
Into air chamber is by gas passage with detecting strong gas connection.When the volume of air chamber changes, whole detection is allowed
The gas volume of intracavitary is also changed, in order to maintain test chamber and outside gas or air pressure balance, it is necessary to by entering
The need for sample mouthful exchanging gas reaches inside and outside air pressure balance.
In addition, testing element can be placed in test chamber, it is of course also possible to not place testing element.Testing element can be with
It is arranged in test chamber, analyte before detecting testing element can also be placed temporarily in sample in advance
In test chamber.
When operation, when test-strips are included in test chamber, air chamber is allowed to be in a stable state, this
When air chamber and test chamber gas connection, their gas volume or the air pressure of inside remain unchanged.After liquid is gathered, allow
Injection port is embedded under liquid sample liquid level, or injection port is sealed by liquid sample, now, and test chamber is constituted with air chamber
The environment of one closing.This when, the volume of air chamber is allowed to reduce, even 0, now, it is previously disposed in the gas of air chamber
Test chamber is pressed into, the gas allowed in test chamber increases, so that pressure increases.The pressure of increase can overcome hydraulic seal
The pressure of injection port, allows a part of gas to be rejected to by injection port outside test chamber.At this time, if the body of increase air chamber
Product, allows test chamber to turn into negative pressure, in order to overcome negative pressure, partially liq enters test chamber by injection port, is contacted with test-strips, complete
Into the test of analyte in sample matter（Fig. 6,）.
It is of course also possible to such operation：When test-strips are included in test chamber, air chamber is allowed to be in a stabilization
State, now air chamber and test chamber gas connection, their gas volume or the air pressure of inside remain unchanged.Allow air chamber
Volume reduce, even 0, now, the gas for being previously disposed in air chamber is pressed into test chamber, the gas allowed in test chamber
Increase, so that pressure increases, the pressure of increase is rejected to outside by injection port, the external and internal pressure of such test chamber reaches flat
Weighing apparatus.After liquid is gathered, injection port is allowed to be embedded under liquid sample liquid level, or injection port is by hydraulic seal, now, inspection
Survey the environment that chamber constitutes a closing with air chamber.At this time, if the volume of increase air chamber, allows test chamber to turn into negative pressure,
In order to overcome negative pressure, partially liq enters test chamber by injection port（Realize the liquid outside the gas-detecting cavity in test chamber
Exchange）, contacted with test-strips, complete the test of analyte in sample matter.
In some preferred modes, the Volume Changes of air chamber are completed by elastomeric material, for example, due to portion gas chamber
Be made up of elastomeric material, when elastomeric material is by external force, the volume of air chamber relative to not by external force when diminish, it is unnecessary
Gas be pressed into test chamber.On the contrary, after external force disappears, the need for self-recovery elasticity, the volume increase of air chamber,
The space of increase allows test chamber to generate negative pressure, and the negative pressure of generation can allow outside liquid sample to enter test chamber by injection port
In contacted with testing element.Concrete principle and method are described as previously described.Diminishing for volume can be the 10% of initial volume, extremely
It is less 20%, at least 35-80%, at least 40-95% can be to allow the volume of air chamber to be reduced to 0.When the volume of air chamber
After diminishing, the gas phase in test chamber should be excluded by injection port.When having hydraulic seal injection port, if now increased
The volume of air body cavity, allows test chamber to be changed into negative pressure, and liquid sample is entered in test chamber by injection port.
In some modes, the Volume Changes of air chamber are completed by elastic flexible material, and external force can be automatically by outside
Equipment addition and automatic disappearance automatically.The Volume Changes of air chamber can also reduce or increase volume by piston like motion.
When the volume of air chamber changes, the gas volume or gas pressure that this change allows in test chamber also occur therewith
The exchange of gas and liquid is realized in change, this change by injection port, so as to realize that liquid sample enters test chamber, Jin Erke
With engaged test element, the chemical examination or detection of analyte in liquid sample are completed.
Elastomeric material can realize the function of the present invention selected from rubber, latex or other conventional elastomeric materials.
In some preferred modes, elastomeric material is latex.
In a specific embodiment, detection means of the invention includes：Lid, lid includes accommodating testing element
Test chamber, the air chamber of injection port and variable volume realizes that the gas in test chamber leads to by the Volume Changes of air chamber
Cross injection port to swap with the liquid sample outside test chamber, so as to allow liquid to enter test chamber.For example, as shown in Figure 3.Lid
100 include accommodating the air chamber 113 of the test chamber 115 of testing element, injection port 120, and variable volume, air chamber and detection
Chamber gas connection, for example, be interconnected by gas passage 114.It is preferred that, this detection means also includes being used to collect liquid-like
This collecting chamber, lid is used to seal the opening of the collecting chamber.When operation, gone to collect liquid-like first with collecting chamber
This is for example collected into the collecting chamber in cup 200 into collecting chamber 202.Then the opening of collecting chamber is sealed with lid 100
201, now, the injection port on lid is not contacted with liquid.Then, cup 200 is tilted, allows liquid sample to seal injection port 120,
Now, because the volume of test chamber and air chamber remains unchanged substantially, almost no liquid is entered in test chamber（Fig. 3）.With
Finger presses air chamber 113, allows its volume to diminish, unnecessary gas is pressed into test chamber 115, causes detection cavity pressure to increase
Greatly, unnecessary gas is rejected in test chamber by injection port 120（Fig. 4）.Then external force disappears, because air chamber is by bullet
Property material constitute, after external force disappears, with the ability for recovering original state, this when, the volume of air chamber 113 increases again,
So as to allow test chamber to turn into negative pressure, in order to balance pressure, liquid sample is entered in test chamber 115 by injection port 120, with inspection
The testing element 102 surveyed in chamber is contacted（Fig. 5）.In a preferred mode, a diameter of 0.1-10 millimeters of injection port,
0.01-0.5 millimeters.Or for 10-500 microns;Allow the injection port that there is certain surface tension, test chamber and collecting chamber it
Between do not have before gas or fluid exchange, reduce liquid, or even prevent liquid entry into test chamber (Fig. 3).Preferred at other
In mode, in one end of injection port 120 and the place in the test chamber 115 is "T"-shaped mouth（The view in cross section）, Er Qiejin
Sample mouthful is in one end of a pipe 150, other end connection " T " word mouthful.Two annexes for being located at " T " word mouthful are the samples of testing element
Receiving area.So, the liquid sample 300 for entering test chamber 115 by injection port 120 goes out to collect in " T " rim of the mouth, so
The liquid sample of smaller size smaller is set fully to be contacted with sample reception region.
In some preferred modes, lid includes upper cover body 101 and lower cover 104, and between lid up and down
Testing element 102, the testing element is located between upper and lower covers in the test chamber 115 that is formed.In one depression of Shang Gaichu formation
Upper lid chamber 109, corresponding caveli body 105 is formed on lower cover, upper lid chamber 109 coordinates with lower cover cavity 105, at them
Between a pedestal or firm banking 107 be set, the opening edge of flexible gas accommodating chamber 108 along being arranged in pedestal, while
Flexible gas accommodating chamber in the passage 114 of the reserved gas circulation of test chamber, the passage 114 connection gas held chamber 108 with
Test chamber 115.Gas held cavity 108 includes top 116 and the side wall 135 of gas held, and top and side wall define gas
Chamber 113.The top 116 of accommodating chamber 108 and side wall 135 are constituted for elastic material.Top 116 is upward is not above lid for this
Upper lid chamber 109 plane.So, when in use, top 116 of the external force to gas held cavity 108 can be applied, allowed
Gas held cavity is deformed upon, and allows the volume of gas held chamber 113 to change, for example, become big or diminish.In preferred side
In formula, the elastomeric material is allowed cavity 113 to there occurs the change of volume by after external force, if after external force disappears, elastomeric material can
Voluntarily to return to original state, so as to allow the volume of air chamber to change again.For example, when original state（Figure
3）, top has layer protecting film 112 to cover in upper lid cavity at top.The effect of the diaphragm is the unexpected extruding of preventing and treating
The top of cavity 113 allows the volume of cavity 113 to change.When needing operation, diaphragm 112 is opened, is then firmly squeezed
Calm the anger the top 116 of body cavity 113, allow the volume of air chamber to diminish（Fig. 4）.After the volume of so air chamber 113 diminishes, original position
It is discharged in the gas or air in air chamber 113 by passage 114 in test chamber 115.It is because the material of test chamber is constituted
Essence is rigid, hardly occurs at such pressures（Almost do not occur at such pressures）Elastic deformation.So examine
The air pressure rise surveyed in chamber 115, in order to keep the air pressure balance inside and outside test chamber, unnecessary gas is excluded by injection port 120
The outside of test chamber 115, for example, be rejected in collecting chamber 202.When external force is eliminated, because top 116 and side wall 135 have
Initial elasticity is returned to, so, the volume of air chamber becomes greatly again, allow in test chamber 115 and generate negative pressure, so as to make way for sample introduction
The liquid sample 300 of mouth 120 enters test chamber 115 and contacted with the testing element 102 in it.Injection port is arranged on lid
Another side, and set with air chamber opposition.
In some modes, one or more necks 103 are included in the test chamber in lid, one is set in each neck
Individual testing element 102, meanwhile, each testing element includes sample reception region 117, detection zone 118 and absorption region 119.
In preferred mode, position corresponding with detection zone, so can be directly by transparent to be transparent in upper cover body 101
Place observation detection zone on reaction or reaction result, so as to obtain test result.
In addition to above description, test chamber can also be located at the side wall of collecting chamber.In addition, air chamber and test chamber and entering
Sample mouthful is located at the side wall of collecting chamber, is connected as a single entity with collecting chamber.Certainly, in other mode, collecting chamber 202 is not this
Invent a necessary part.
In other mode, the bottom of collecting chamber 202 is projection 204, and the prominent role is to work as liquid sample very
When few, liquid sample can be allowed to collect when cavity is tilted, the height of injection port can be reached, so can be with
Satisfaction allows liquid sample to seal injection port.
Testing element
Testing element can select the test strip of lateral flow, and it can detect a variety of analytes.Certainly, other are closed
Suitable testing element can also be used in the present invention.
Various testing elements can be grouped together and apply in the present invention.A kind of form is Test paper.For dividing
Analyse the analyte in sample（Such as drugs or the metabolin for showing health）Test paper can be various forms, such as exempt from
The form of epidemic disease measure or chemical analysis.Test paper can be using non-competing method or the analytical model of competition law.Test paper bag
Containing an absorbent material with sample application zone, reagent area and test section.Sample-adding passes through capillarity originally to sample application zone
Flow to reagent area.In reagent area, if there is analyte, sample is combined with reagent.Then sample continues to flow to detection zone.
Other reagents, such as molecule with the Non-specific combination of analyte are fixed on detection zone.These reagents and being divided in sample
Analyse thing（If there is）React and combine analyte and combined in the area, or with some reagent of reagent area.For showing
Show that the label of detection signal is present and reagent area or the mark zone separated.
Typical non-competing method analytical model is that signal will be produced if containing analyte in sample, if do not wrapped
Containing analyte, signal is not just produced.In competition law, if analyte is not present in sample, signal is produced, if deposited
In analyte, then signal is not produced.
Testing element is Test paper, can be from water suction or the material not absorbed water.Test paper may include multiple material
For liquid sample transmission.The material of one of which Test paper can be covered on another material, as filter paper is covered in nitric acid
On cellulose membrane.One area of Test paper can be from one or more materials, and another area selects other different one kind
Or multiple material.Test paper, which can be attached on certain holder or hard surface, to be used for raising and is affectedly bashful the strong of Test paper
Degree.
Analyte is detected by signal generating system, such as utilizes one with this analyte generation specific reaction
Plant or a variety of enzymes, using such as foregoing method being fixed on specific combinating substance on Test paper, one or more signals are sent out
The composition of raw system is fixed on the analyte detection zone of Test paper.Produce signal material can in sample application zone, reagent area,
Or on detection zone, or whole Test paper, the material can be full of on one or more materials of Test paper.Signal will be contained
The solution of thing is added to the surface of test paper or one or more materials of test paper is immersed in the solution of the thing containing signal.Contain addition
The test paper of signal thing solution is dried.
Each area of Test paper can be arranged in the following manner：Sample application zone, reagent area, detection zone, control zone determines sample
This whether adulterated area, liquid sample uptake zone.Control zone is located at after detection zone.All areas can be arranged at only with a kind of
On one test paper of material.It may also be not same district and use different materials.Each area directly can be contacted with liquid sample, or not
The direction arrangement that same area is flowed according to liquid sample, the end in each area is connected and overlapped with the front end in another area.Used
Material can be the preferable material of water imbibition such as filter paper, glass or nitrocellulose filter etc..Test paper can also use it
His form.
Embodiment
The embodiment of the present invention is described in detail presently in connection with accompanying drawing, this explanation is not to this hair
Bright claim carries out any limitation, but for illustrating how to realize the present invention, belongs to the concrete mode of the present invention.
Fig. 1-5 is the specific embodiment of the present invention.For example shown in Fig. 1, detection means includes lid, Yi Ji
The test chamber and the cavity and injection port of receiving gas formed in lid.Lid includes upper cover body 101 and lower cover 104, with
And positioned at testing element 102 up and down between lid, the testing element is located in the test chamber 115 formed between upper and lower covers.
The upper lid chamber 109 of a depression is formed at upper lid, corresponding caveli body 105, upper lid chamber 109 and lower cover are formed on lower cover
Cavity 105 coordinates, and a pedestal or firm banking 107 are set between them, and the opening edge edge of flexible gas accommodating chamber 108 is set
Put in pedestal, while in flexible gas accommodating chamber in the passage 114 of the reserved gas circulation of test chamber, the passage 114 connects
Connect gas held chamber 108 and test chamber 115.Gas held cavity 108 includes top 116 and the side wall 135 of gas held, top
Air chamber 113 is defined with side wall.The top 116 of accommodating chamber 108 and side wall 135 are constituted for elastic material.The top 116 is upward
Projection is not above the plane of the upper lid chamber 109 of lid.Detection means is formed after lid assembling.
Simultaneously there is provided the container of a collection liquid sample, such as cup 200, the cup includes opening 201 and collecting chamber
202 and bottom 203, lid can be for closed or sealing opening 201.Sealing ring 110 is also set up on lid.What is used
When, first with collection cup collection liquid body sample, such as urine.Then lid is covered in opening, then tilts whole dress
Put, such as Fig. 3.So, collect in collecting chamber of the liquid sample in cup, and flood injection port 120, or sealing sample introduction
Mouthful.This when, whole test chamber is sealed as the space of a closing, and because external and internal pressure is consistent, liquid sample is hardly
Into in test chamber 115.This when, diaphragm 112 is opened, allows the top 116 of air chamber of flexible deformation to expose,
With finger or outside machine tool extruding top 116, the volume of air chamber 113 is allowed to diminish.Allow in the presence of the gas with air chamber 113
Body is entered in test chamber 115 by passage 114, and the air pressure rise in such test chamber, the unnecessary gas of elevated air pressure passes through
Injection port 120 is rejected in collecting chamber（Fig. 4）.Then external force is eliminated, because elastomeric material has the ability for recovering reset condition,
The volume of air chamber is allowed to become big, the volume for becoming big needs more gases to enter air chamber, the gas so allowed in test chamber 115
Into in air chamber, test chamber is allowed to become negative pressure, due to pressure reduction, and the pressure of test chamber is higher than the pressure in test chamber
Power, so, liquid sample enter in test chamber and contacted with the testing element in test chamber, and liquid sample is balanced into test chamber
Pressure differential between test chamber and collecting chamber.Inspection this completes testing element to analyte in liquid sample
Survey.Can be obtained by transparent upper cover body or read test region testing result.
Claims (21)
1. the detection means of analyte in a kind of liquid sample, including：Test chamber for accommodating testing element；Sample introduction
Mouthful；One gas allowed in test chamber carries out the equipment that gas is exchanged with the gas outside test chamber by injection port；Described gas
Body alternating current equipment includes the air chamber of the receiving gas of variable volume；Described air chamber is connected with test chamber by gas passage
It is logical；The air pressure that described air chamber allows in test chamber and the air pressure outside test chamber produce pressure differential, and the pressure differential forces test chamber
Outer liquid sample is entered in test chamber；Produced in described test chamber before pressure differential, injection port is by outside test chamber
Liquid sample sealing；The liquid sample outside test chamber is located in the collecting chamber for collecting fluid sample；The gas
The air pressure that the Volume Changes of cavity allow in test chamber changes, so as to cause the air pressure in described test chamber and outside test chamber
Difference.
2. detection means according to claim 1, wherein, described air chamber includes elastic flexible material.
3. detection means according to claim 1, wherein, described variable volume includes the change of volume greatly or diminished.
4. detection means according to claim 1, wherein, described variable volume includes subtracting from initial volume to volume
It is few, then become big again from the volume after reduction.
5. detection means according to claim 2, wherein, described elastic flexible material is self elastically-deformable material
Material, deformation of elastic element is allowed by external force, so that the volume allowed in air chamber diminishes, so that the air pressure increase allowed in test chamber,
The gas that the air pressure of increase is made way in test chamber is rejected to outside test chamber from injection port.
6. detection means according to claim 5, wherein, after external force disappearance or partial disappearance, flexible member is returned to
Original state, the volume of air chamber becomes big from small, allows test chamber is interior to form negative pressure, negative pressure forces liquid sample to enter by injection port
Enter in test chamber.
7. the detection means according to one of claim 1-5, wherein, include being used in test liquid samples in test chamber
The testing element of analyte.
8. detection means according to claim 7, wherein, the sample application zone of described testing element is close to sample introduction
Mouthful.
9. the detection means of analyte in a kind of liquid sample, including：Liquid sample collection chamber；One is used to seal collection
The lid of chamber opening, the lid includes the test chamber for being used to accommodate testing element；Injection port；The air chamber of one receiving gas,
Described air chamber is connected with test chamber by gas passage；The air pressure allowed by the Volume Changes of air chamber in test chamber and inspection
The air pressure surveyed outside chamber produces pressure differential, and the pressure differential forces the liquid sample in collecting chamber to enter inspection by described injection port
Survey in chamber；Before the draught head described in formation, liquid sample sealing injection port；The volume of air chamber to be variable, volume
Change generates described draught head.
10. detection means according to claim 9, wherein, described injection port and air chamber is respectively set at lid
Both sides.
11. detection means according to claim 10, wherein, described air chamber includes elastic flexible material.
12. detection means according to claim 11, wherein, described variable volume includes the change of volume greatly or diminished.
13. detection means according to claim 12, wherein, described variable volume is included from initial volume to volume
Reduce, then become big again from the volume after reduction.
14. detection means according to claim 13, wherein, described elastic flexible material is self elastically-deformable material
Material, deformation of elastic element is allowed by external force, so that the volume allowed in air chamber diminishes, so that the air pressure increase allowed in test chamber,
The gas that the air pressure of increase is made way in test chamber is rejected to outside test chamber from injection port.
15. detection means according to claim 14, wherein, after external force disappearance or partial disappearance, flexible member is replied
To original state, the volume of air chamber becomes big from small, allows test chamber is interior to form negative pressure, negative pressure forces liquid sample to pass through injection port
Into in test chamber.
16. a kind of method for detecting analyte in liquid sample, this method includes：
Detection means is provided, the detection means includes：Test chamber for accommodating testing element；Injection port；One allows test chamber
Interior gas carries out the air chamber that gas is exchanged with the gas outside test chamber by injection port, and described air chamber is led to test chamber
Cross gas passage connection；By gas exchanges by test chamber outside liquid sample test chamber entered by described injection port
In；
The volume of air chamber is allowed to reduce, the excessive gas made way in test chamber is rejected to outside test chamber by injection port；
The volume of air chamber is allowed to increase again, the volume of increase allows test chamber is interior to form negative pressure, and the negative pressure of the formation, which is forced, to be located at
Liquid at injection port enters test chamber；
The volume of air chamber is allowed to increase again before excessive gas in test chamber is rejected to outside test chamber or again, increase
Volume allows test chamber is interior to be formed before negative pressure, allows liquid sample to contact injection port, so as to seal injection port；
The volume of air chamber is variable；
The change of air chamber volume makes test chamber interior with producing draught head outside test chamber, and described draught head forces liquid sample to lead to
Injection port is crossed to enter in test chamber.
17. method according to claim 16, wherein, described air chamber includes elastic flexible material.
18. method according to claim 17, wherein, described elastic flexible material is self elastically-deformable material,
Deformation of elastic element is allowed by external force, so that the volume allowed in air chamber diminishes, so that the air pressure increase allowed in test chamber, increase
The gas made way in test chamber of air pressure be rejected to from injection port outside test chamber.
19. method according to claim 18, wherein, after external force disappearance or partial disappearance, flexible member is returned to just
Beginning state, the volume of air chamber becomes big from small, allows test chamber is interior to form negative pressure, negative pressure forces liquid sample to enter by injection port
In test chamber.
20. the method according to one of claim 16-19, wherein, include being used in test liquid samples in test chamber
The testing element of analyte.
21. method according to claim 20, wherein, the sample application zone of described testing element is close to injection port.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310502781.XA CN104569300B (en) | 2013-10-22 | 2013-10-22 | The detection means of analyte in a kind of liquid sample |
| PCT/CN2014/088102 WO2015058612A1 (en) | 2013-10-22 | 2014-10-05 | A testing device for testing analytes in liquid samples |
| US15/030,852 US10495635B2 (en) | 2013-10-22 | 2014-10-05 | Testing device for testing analytes in liquid samples |
| HK16110928.8A HK1222597A1 (en) | 2013-10-22 | 2014-10-05 | A testing device for testing analytes in liquid samples |
| CN201480055239.7A CN105612001B (en) | 2013-10-22 | 2014-10-05 | A detection device for analyte in a liquid sample |
| EP14856723.3A EP3060343A4 (en) | 2013-10-22 | 2014-10-05 | A testing device for testing analytes in liquid samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310502781.XA CN104569300B (en) | 2013-10-22 | 2013-10-22 | The detection means of analyte in a kind of liquid sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104569300A CN104569300A (en) | 2015-04-29 |
| CN104569300B true CN104569300B (en) | 2017-07-28 |
Family
ID=53085857
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310502781.XA Expired - Fee Related CN104569300B (en) | 2013-10-22 | 2013-10-22 | The detection means of analyte in a kind of liquid sample |
| CN201480055239.7A Expired - Fee Related CN105612001B (en) | 2013-10-22 | 2014-10-05 | A detection device for analyte in a liquid sample |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480055239.7A Expired - Fee Related CN105612001B (en) | 2013-10-22 | 2014-10-05 | A detection device for analyte in a liquid sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN104569300B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017201723A1 (en) * | 2016-05-27 | 2017-11-30 | 卓金星 | Microorganism detection system |
| CN109085159B (en) * | 2017-08-21 | 2024-09-06 | 上海荒岛科技有限公司 | A substrate and a detection card |
| WO2019143319A1 (en) | 2018-01-16 | 2019-07-25 | Hewlett-Packard Development Company, L.P. | Fluid testing |
| CN116990519B (en) * | 2023-09-28 | 2023-11-28 | 山东省立第三医院 | Portable blood sugar detector container |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6786106B2 (en) * | 2000-10-24 | 2004-09-07 | Kenneth Alley | Apparatus for sampling and testing a specimen |
| CN101852689B (en) * | 2003-11-14 | 2012-07-18 | 阿莱瑞士股份有限公司 | Sample collection cup with integrated sample analysis system |
| CN2724003Y (en) * | 2004-08-20 | 2005-09-07 | 杨秀红 | Piston pumping type liquid sampling seal detector |
| CN2864667Y (en) * | 2005-10-25 | 2007-01-31 | 艾康生物技术(杭州)有限公司 | Detection device for liquid samples |
| CN101498655B (en) * | 2005-10-25 | 2012-05-30 | 阿莱瑞士股份有限公司 | Detecting instrument for liquid sample |
| US7879623B2 (en) * | 2006-03-31 | 2011-02-01 | Guirguis Raouf A | Integrated device for analyte, testing, confirmation, and donor identity verification |
| DE202007004797U1 (en) * | 2007-03-30 | 2007-06-14 | DIMA Ges. für Diagnostika GmbH | Container for collecting and analyzing human urine comprises a passage valve structured so that a volume of a liquid sample is able to flow into a test chamber when the valve is in the open position |
| EP2190580A1 (en) * | 2007-08-27 | 2010-06-02 | 3M Innovative Properties Company | Apparatus and method for processing a fluidic sample |
| CN201156047Y (en) * | 2008-03-04 | 2008-11-26 | 艾博生物医药(杭州)有限公司 | Cup cover for sample detection |
| CN201464268U (en) * | 2009-04-23 | 2010-05-12 | 艾博生物医药(杭州)有限公司 | Liquid sample collection and detection cup |
| CN102998149A (en) * | 2011-09-16 | 2013-03-27 | 江苏康健医疗用品有限公司 | Negative pressure urine collecting device |
| CN203587596U (en) * | 2013-10-22 | 2014-05-07 | 艾博生物医药(杭州)有限公司 | Detection device |
-
2013
- 2013-10-22 CN CN201310502781.XA patent/CN104569300B/en not_active Expired - Fee Related
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2014
- 2014-10-05 CN CN201480055239.7A patent/CN105612001B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN105612001B (en) | 2018-06-26 |
| CN104569300A (en) | 2015-04-29 |
| CN105612001A (en) | 2016-05-25 |
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