CN104432001A - Edible composition and use thereof - Google Patents

Abstract

The invention discloses an edible composition, which comprises a probiotic culture, wherein the probiotic culture is obtained by cultivating the probiotic with a culture medium. The edible composition according to the invention has the functions of regulating intestinal flora, lowering blood sugar, lowering blood fat and improving immunity.

Description

Edible compositions and uses thereof

technical field

The present invention relates to the field of food, in particular, the present invention relates to edible compositions and uses thereof.

Background technique

Probiotics are a type of active microorganisms that are beneficial to the host. They colonize the human intestinal tract and reproductive system. Common beneficial bacteria in the human body mainly include Clostridium butyricum, lactic acid bacteria, and bifidobacteria. Probiotics have beneficial physiological effects on the host by regulating the host's mucosal and systemic immune functions and improving the balance of intestinal nutrition and flora, thereby improving the composition of the host's intestinal flora, promoting the digestion and absorption of nutrients, and inhibiting harmful bacteria and harmful substances and other functions.

At present, hundreds of probiotic products have been developed abroad, including general food, health care products, and medicines, including yoghurt, yogurt, sour milk and oral liquid containing probiotics , tablets, capsules, powders, antibacterial sprays, etc. However, the combination administration of probiotic culture as adjuvant has not been developed yet.

Contents of the invention

The present invention aims to solve one of the above-mentioned technical problems at least to a certain extent. Therefore, an object of the present invention is to provide an edible composition comprising a probiotic culture, which has the effects of regulating intestinal flora, lowering blood sugar, lowering blood fat and improving immunity.

The existing probiotic health care products or medicines all use simple bacteria as raw materials, or add polyfructosaccharides, cottonseed galacto-oligosaccharides, xylo-oligosaccharides and other prebiotics for joint administration. However, the combination administration of probiotic culture as adjuvant has not been developed yet. The inventors of the present invention have found that the cultures in the probiotic culture solution are rich in nutrition, and the metabolites, vitamins, amino acids, digestive enzymes, organic acids, polysaccharides and unknown growth factors of the probiotics contained in the probiotic culture solution can effectively promote intestinal Beneficial bacteria multiply and improve the gastrointestinal flora of animals.

To this end, in one aspect of the present invention, the present invention proposes an edible composition. According to a specific embodiment of the present invention, the composition includes a probiotic culture, wherein the probiotic culture is obtained by cultivating the probiotic with a culture medium. The edible composition combined with probiotics and its culture solution is significantly better than the combination of probiotics alone or probiotics and prebiotics in terms of regulating the proliferation of beneficial bacteria in the human intestinal tract, lowering blood sugar, and lowering blood lipids. combination.

The edible composition of the present invention adopts a combination of probiotics and their culture solution, all raw materials are taken from nature, does not contain any chemical preparations, has no toxic and side effects, is safe and reliable; and the sources of raw materials are widely available, and the compatibility of the formula is scientific and reasonable, and the curative effect is excellent. Indeed, it has a synergistic effect and can be effectively used to regulate intestinal flora, lower blood sugar, lower blood lipids, etc.

According to a specific embodiment of the present invention, the edible composition of the present invention has been proven to have no toxic and side effects through crowd consumption tests, and has significant effects of lowering blood sugar, lowering blood lipids, regulating intestinal flora, improving immunity, etc., and meets the requirements of health care products. requirements and trends. The edible composition of the present invention can significantly reduce the content of serum total cholesterol and triglyceride in the blood, increase the level of high-density lipoprotein, and regulate intestinal flora; decline. This composition is suitable for people with high blood sugar, high blood fat, obesity and the elderly. Through daily use, from the perspective of treating the root cause, it can finally achieve the purpose of protecting health by improving lipid metabolism, improving blood flow, and improving human immunity. .

In addition, the edible composition of the present invention achieves convenient and significant health effects such as lowering blood sugar, lowering blood fat, and improving immunity of the body through the rational combination of pure natural raw materials. Stable, the product can be stored for a long time, therefore, the edible composition can provide a new choice for people's daily health care.

According to an embodiment of the present invention, the probiotics contained in the composition can be probiotics of any genus, and according to a specific embodiment of the present invention, the probiotics can be selected from the group consisting of Faecalibacterium, Eubacterium (Eubacterium), Roseburia, Coprococcus, Bifidobacterium, Butyrivibrio, Bactobacillus, Lactococcus ) at least one of. Therefore, the efficacy of the edible composition in regulating the proliferation of beneficial bacteria in the human intestinal tract, lowering blood sugar, lowering blood fat and the like can be further improved.

According to an embodiment of the present invention, the specific probiotics contained in the composition are not particularly limited. According to a specific embodiment of the present invention, the probiotics therein can be selected from the group consisting of Eubacterium rectal (Eubacterium rectal), Glucose Rose Roseburia inulinivorans, Roseburia intestinalis, Faecalibacterium prausnitzii, Clostridium butyricum, Bacillus coagulans, Bifidobacterium bifidus, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescentis, Lactobacillus delbrueckii bulgaricus, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus casei subspecies, Lactobacillus reuteri, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus salivarius, Fischeri At least one of Propionibacterium subsp. sherbeckeri, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis (subsp. diacetyl), and Leuconostoc enteritidis subsp. enterica. Therefore, the efficacy of the edible composition in regulating the proliferation of beneficial bacteria in the human intestinal tract, lowering blood sugar, lowering blood fat and the like can be further improved.

According to an embodiment of the present invention, the type of the above-mentioned medium is not particularly limited. According to a specific embodiment of the present invention, the medium can be a solid medium or a liquid medium, and the components contained in the medium are not limited. Particularly limited, according to specific embodiments of the present invention, the culture medium may contain carbon sources, nitrogen sources, growth factors, inorganic salts and water. According to a specific embodiment of the present invention, the carbon source can be at least one selected from sugars, organic acids, alcohols, and lipids, and the nitrogen source can be selected from proteins and their degradation products in varying degrees, ammonium salts, and nitrates. At least one of, and the growth factor can be at least one selected from vitamins, amino acids, purine and pyrimidine bases. This can further improve the quality of life of probiotics, so that the edible composition contains as many live probiotics as possible, so as to further improve the ability of the edible composition to regulate the proliferation of beneficial bacteria in the human intestinal tract, lower blood sugar, and lower blood lipids. Etc. effects.

According to an embodiment of the present invention, the above-mentioned edible composition can be prepared into any dosage form. According to a specific embodiment of the present invention, the edible composition can be in the form of capsule or oral liquid, which can be more convenient to eat, so that Play its role in regulating the proliferation of beneficial bacteria in the human intestinal tract, lowering blood sugar, and lowering blood lipids.

In another aspect of the invention, the invention provides a food product. According to a specific embodiment of the present invention, the food product comprises the aforementioned edible composition. According to a specific embodiment of the present invention, by eating the food in daily life, the effects of regulating the proliferation of beneficial bacteria in the human intestinal tract, lowering blood sugar, and lowering blood lipids can be achieved. Thus, the food may be a functional food or a health food.

In yet another aspect of the present invention, the present invention proposes the use of the aforementioned edible composition in the preparation of preparations. According to specific embodiments of the present invention, the preparations can be used to regulate intestinal flora, lower blood sugar, lower Blood lipids and at least one of improving immunity. Because the preparation contains the above-mentioned edible composition, since the composition can increase the quantity of probiotics in the intestinal tract, and further have the function of regulating the intestinal flora. Therefore, using the edible composition to prepare a preparation can effectively improve the effects of the preparation on regulating intestinal flora, lowering blood sugar, lowering blood fat, and improving immunity.

In yet another aspect of the present invention, the present invention proposes the use of the aforementioned edible composition or food in regulating the intestinal flora of animals. The inventors found that by eating the above-mentioned edible composition or food, the number of probiotics in the intestinal tract of animals can be significantly increased, and the purpose of effectively regulating the balance of intestinal flora can be achieved. Therefore, eating the edible composition or food can be effectively used to treat and prevent diseases such as enteritis and diarrhea caused by intestinal flora imbalance.

In yet another aspect of the present invention, the present invention proposes a method for regulating intestinal flora. According to a specific embodiment of the present invention, the method is to supply the aforementioned edible composition or food to animals. According to a specific embodiment of the present invention, the method can increase the number of probiotics in the intestinal tract of the supplied animal, effectively regulate the balance of the intestinal flora of the animal, and then effectively regulate the blood sugar and blood pressure of the animal through the function of healthy intestinal flora And the purpose of improving immunity.

Additional aspects and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.

Detailed ways

The present invention will be described below with reference to specific embodiments. It should be noted that these embodiments are only illustrative and do not limit the present invention in any way.

The preparation of embodiment 1 probiotic culture medium

1. Culture medium of Clostridium butyricum and Clostridium butyricum:

Tryptic casein: 5.0g

Peptone (pepsin digested): 5.0g

Yeast paste: 10.0g

Resazurin: 1.0mg

_{CaCl2x2H2O :} 0.25g

_{MgSO4x7H2O :} 0.5g

K ₂ HPO ₄ : 1.0 g

KH ₂ PO ₄ : 1.0g

NaHCO ₃ : 10.0g

NaCl: 2.0g

Cysteine-HCl x _H2O : 0.5g

Add ddH ₂ 0 to make the volume to 1L, and adjust the pH to 7.0.

2. The third Clostridium medium:

Beef extract: 20.0g

Peptone: 30.0g

Yeast paste: 5.0g

K ₂ HPO ₄ : 5.0 g

Resazurin: 1.0mg

Glucose: 4.0g

Cellobiose: 1.0g

Maltose: 1.0g

Soluble starch: 1.0g

Add ddH ₂ O to make the volume to 1 L, and adjust the pH to 7.0.

3. Rose Berry culture medium

Tryptic casein: 10.0g

Yeast paste: 5.0g

Gravy: 5.0g

Soy flour peptone: 5.0g

Glucose: 5.0g

K ₂ HPO ₄ : 3.0 g

_{MgSO4x7H2O :} 0.7g

_MnSO4 x _H20 : 0.05g

Tween80: 1.0ml

NaCl: 7.00g

_{CaCl2x2H2O :} 0.25g

KH ₂ PO ₄ : 1.0g

NaHCO ₃ : 10.0g

Cysteine-HCl x _H2O : 0.5g

Resazurin (25mg/100ml): 4.0ml

Add ddH ₂ O to make the volume to 1 L, and adjust the pH to 6.8.

4. Lactobacillus reuteri culture medium:

Tryptic casein: 10.0g

Gravy: 10.0g

Yeast paste: 5.0g

Glucose: 20.0g

Tween80: 1.0g

K ₂ HPO ₄ : 2.0 g

Na-acetate: 5.0g

(NH ₄ ) ₂ citrate: 2.0g

Add ddH ₂ O to make up to 1L, and adjust the pH to 6.2-6.5.

5. Butyric acid bacteria, butyric acid bacteria medium

Corn starch: 10.0g

Beef peptone: 10.0g

Yeast paste: 5.0g

Sodium acetate: 3.0g

NaCl: 5.0g

CaCO ₃ : 3.0g

MgSO ₄ x7H ₂ : 0.5g

Cysteine-HCl x _H2O : 0.5g

Resazurin (25mg/100ml): 4.0ml

Add ddH ₂ O to make the volume to 1 L, and adjust the pH to 7.0.

6. Eubacterium rectal culture medium

Tryptic casein: 5.0g

Peptone: 5.0g

Yeast paste: 10.0g

Beef extract: 5.0g

Glucose: 5.0g

K ₂ HPO ₄ : 3.0 g

Tween80: 1.0ml

Cysteine-HCl x _H2O : 0.5g

Resazurin: 1.00mg

_{CaCl2x2H2O :} 0.25g

MgSO ₄ x7H ₂ O: 0.50g

KH ₂ PO ₄ : 1.0g

NaHCO ₃ : 10.0g

NaCl: 2.00g

Haemin solution: 10.0ml

Vitamin _K1 solution: 0.2ml

Add ddH ₂ O to make the volume to 1 L, and adjust the pH to 7.0.

7. Faecalibacterium prausnitzii culture medium

KH ₂ PO ₄ : 6.0g

NaCl: 12.0g

(NH4) ₂ SO ₄ : 6.0g

_{CaCl2x2H2O :} 1.6g

_{MgSO4x7H2O :} 2.5g

K ₂ HPO ₄ : 0.3 g

Tryptic casein: 2.0g

Yeast paste: 0.5g

Volatile fatty acid mixture: 3.1ml

Hemin: 1.0mg

Glucose: 0.5g

Maltose: 0.5g

Cellobiose: 0.5g

Soluble starch: 0.5g

Glycerin: 0.5g

Na ₂ CO ₃ : 4.0g

Resazurin: 1.0mg

Cysteine-HCl x _H2O : 0.25g

_{Na2Sx9H2O :} 0.25g

Add ddH ₂ O to make up to 1L, adjust pH6.7-6.8

Volatile Fatty Acid Blend:

Acetic acid: 4.25ml

Propionic acid: 1.50ml

Butyric acid: 1.00ml

n-valeric acid: 0.25ml

Isobutyric acid: 0.25ml

DL-2-methylbutyric acid: 0.25ml

Isovaleric acid: 0.25ml

The preparation of embodiment 2 probiotics and composition thereof

1. Preparation of Live Clostridium Butyricum Capsules

Cultivate Clostridium butyricum species with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating. Packed in No. 0 capsules to obtain Clostridium butyricum capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

2. Preparation of live Clostridium butyricum capsules containing polyfructose

Cultivate Clostridium butyricum with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, add polyfructose according to the number of viable bacteria of Clostridium butyricum, so that the number of viable bacteria is not less than 1.0* 10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and packed in No. 0 capsules to obtain Clostridium butyricum capsules.

3. Preparation of live Clostridium butyricum oral solution

Clostridium butyricum is inserted into the liquid seed culture medium, and anaerobically cultivated at a suitable temperature to obtain a probiotic seed liquid. Put the probiotic seed solution into the sterilized Clostridium butyricum fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, leave it for anaerobic cultivation, filter it, and aseptically fill it to obtain the product , the number of live bacteria is not less than 1.0*10 ⁸ CFU/ml.

4. Preparation of Live Butyric Bacteria Capsules

Cultivate butyric acid bacteria strains with liquid or solid medium, collect and wash the bacteria, and dry to prepare active bacterial powder; use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating, and use 0 No. capsule packaging, to obtain butyric acid bacteria capsules, the number of bacteria is not less than 1.0*10 ⁸ CFU/g.

5. Preparation of Live Butyric Bacteria Capsules Containing Polyfructose

Cultivate butyric acid bacteria with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, add polyfructose according to the number of viable bacteria of butyric acid bacteria, so that the number of viable bacteria is not less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and packed in No. 0 capsules to obtain butyric acid bacteria capsules.

6. Preparation of Butyric Bacteria Live Bacteria Oral Solution

The butyric acid bacterium is inserted into the liquid seed culture medium and cultured anaerobically at a suitable temperature to obtain a probiotic seed liquid. Put the probiotic seed liquid into the sterilized butyric acid bacteria fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, leave it for anaerobic cultivation, filter it, and aseptically fill it to obtain the product. The number of viable bacteria is not less than 1.0*10 ⁸ CFU/ml.

7. Preparation of Live Butyric Bacteria Capsules

Cultivate butyric acid bacteria with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating, use 0 No. capsule packaging, to obtain butyric acid bacteria capsules, the number of viable bacteria is not less than 1.0*10 ⁸ CFU/g.

8. Preparation of Live Butyric Bacteria Capsules Containing Polyfructose

Cultivate butyric acid bacteria with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, add polyfructose according to the number of viable bacteria of butyric acid bacteria, so that the number of viable bacteria is not less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and packed in No. 0 capsules to obtain butyric acid bacteria capsules.

9. Preparation of Butyric Bacteria Live Bacteria Oral Solution

The butyric acid bacterium is inserted into the liquid seed culture medium and cultured anaerobically at a suitable temperature to obtain a probiotic seed liquid. Put the probiotic seed liquid into the sterilized butyric acid bacteria fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, leave it for anaerobic cultivation, filter it, and aseptically fill it to obtain the product. The number of viable bacteria is not less than 1.0*10 ⁸ CFU/ml.

10. Preparation of live Clostridium capsules

Cultivate Clostridium tertiary strains with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating, Packed in No. 0 capsules to obtain Clostridium III capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

11. Preparation of Live Clostridium Capsules Containing Polyfructose

Cultivate Clostridium tertiary strains with liquid or solid medium, collect and wash the thallus, and dry to prepare active bacteria powder. According to the number of live bacteria of Clostridium tertiary, polyfructose is added to make the number of live bacteria not less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and it is packed in No. 0 capsules to obtain the third capsule Bacteria Capsules.

12. Preparation of Clostridium tertiary viable oral solution

The Clostridium tertiary bacteria is inserted into the liquid seed culture medium, and anaerobically cultured at a suitable temperature to obtain a probiotic seed liquid. The probiotic seed solution is inserted into the sterilized Clostridium tertiary fermentation medium according to the total inoculation amount of 1-3%, then sealed with a plastic film, filtered after static anaerobic cultivation, and aseptically filled to obtain The product, the number of live bacteria is not less than 1.0*10 ⁸ CFU/ml.

13. Preparation of live Clostridium butyricum capsules

Cultivate Clostridium butyricum strains with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, use gelatin to make capsule material, use PVP as subcoat, and then use beeswax for outer coating, Packed in No. 0 capsules to obtain Clostridium butyricum capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

14. Preparation of live Clostridium butyricum capsules containing polyfructose

Cultivate Clostridium butyricum species with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, add polyfructose according to the number of viable bacteria of Clostridium butyricum, so that the number of viable bacteria is not less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and it is packaged in No. 0 capsules to obtain shuttlebutyrate Bacteria Capsules.

15. Preparation of live Clostridium butyricum oral solution

The Clostridium butyricum is inserted into the liquid seed culture medium, and anaerobically cultured at a suitable temperature to obtain a probiotic seed liquid. The probiotic seed solution is inserted into the sterilized Clostridium butyricum fermentation medium according to the total inoculation amount of 1-3%, then sealed with a plastic film, left to stand for anaerobic cultivation, filtered, and aseptically filled to obtain The product, the number of live bacteria is not less than 1.0*10 ⁸ CFU/ml.

16. Preparation of Live Lactobacillus reuteri Capsules

Cultivate Lactobacillus reuteri strains with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacterial powder, use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating , packaged in No. 0 capsules to obtain Lactobacillus reuteri capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

17. Preparation of Live Lactobacillus reuteri Capsules Containing Polyfructose

Cultivate Lactobacillus reuteri with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacteria powder, add polyfructose according to the number of viable bacteria of Lactobacillus reuteri, so that the number of viable bacteria It is less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and it is packed in No. 0 capsules to obtain Luo Lactobacillus ieffi Capsules.

18. Preparation of Lactobacillus reuteri Live Bacteria Oral Solution

The Lactobacillus reuteri is inserted into the liquid seed culture medium, and anaerobically cultured at a suitable temperature to obtain a probiotic seed liquid. Put the probiotic seed liquid into the sterilized Lactobacillus reuteri fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, leave it for anaerobic cultivation, filter it, and aseptically fill it. The obtained product has a viable count of not less than 1.0*10 ⁸ CFU/ml.

19. Preparation of live capsules of Faecalibacterium prausnitzii

Cultivate Faecalibacterium prausnitzii with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacterial powder, use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating , packaged in No. 0 capsules to obtain Faecalibacterium prausnitzii capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

20. Preparation of live capsules of Faecalibacterium prausnitzii containing polyfructose

Cultivate the strains of Faecalibacterium prausnitzii with liquid or solid medium, collect and wash the bacteria, and dry to prepare active bacteria powder. It is less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and it is packed in No. 0 capsules to obtain general Faecalibacterium shizi Capsules.

21. Preparation of live oral solution of Faecalibacterium prausnitzii

The Faecalibacterium prausnitzii was inserted into the liquid seed medium, and anaerobically cultured at a suitable temperature to obtain a probiotic seed liquid. Put the probiotic seed liquid into the sterilized Faecalibacterium prausnitzii fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, leave it for anaerobic cultivation, filter it, and aseptically fill it. The obtained product has a viable count of not less than 1.0*10 ⁸ CFU/ml.

22. Preparation of Live Fungus Rectum Capsules

Cultivate the fungal strains of rectal bacteria with liquid or solid medium, collect and wash the bacteria, dry to prepare active bacterial powder, use gelatin to make capsule material, use PVP as the subcoat, and then use beeswax for outer coating. Packed in No. 0 capsules to obtain Eubacterium rectal capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

23. Preparation of Live Eubacterium rectal Capsules Containing Polyfructose

Cultivate the fungal strains of rectal bacteria with liquid or solid medium, collect and wash the bacteria, and dry them to prepare active bacterial powder. According to the number of viable bacteria of rectal fungi, polyfructose is added to make the number of viable bacteria not less than 1.0* 10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and packed in No. 0 capsules to obtain Eubacterium rectal capsules.

24. Preparation of Oral Solution of Eubacterium Rectum Live Bacteria

Introduce Eubacterium rectum into the liquid seed culture medium and culture it anaerobically at a suitable temperature to obtain probiotic seed liquid. Put the probiotic seed solution into the sterilized Eubacterium rectal fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, leave it for anaerobic cultivation, filter it, and aseptically fill it to obtain the product , the number of live bacteria is not less than 1.0*10 ⁸ CFU/ml.

25. Preparation of live bacteria capsules of Rosborella enterica

Enterobacteriaceae Rosie is cultured with liquid or solid medium, collected and washed, dried to prepare active bacterial powder, made of gelatin as a capsule material, PVP is used as a subcoat, and beeswax is used for the outer layer Coating and packaging with No. 0 capsules to obtain Bairrellia enterica capsules, the number of live bacteria is not less than 1.0*10 ⁸ CFU/g.

26. Preparation of live bacterial capsules containing polyfructose Rosybacteria enterica

Enterobacteriaceae Rossi are cultured with liquid or solid medium, the bacteria are collected and washed, dried to prepare active bacteria powder, and polyfructose is added according to the number of viable bacteria of enterobacteriaceae, The number of viable bacteria is not less than 1.0*10 ⁸ CFU/g, the amount of polyfructose added is not less than 4g, the capsule material is made of gelatin, the subcoat is made of PVP, and the outer layer is coated with beeswax, and the No. 0 capsule is used Packed to get Rosyboria enterica capsules.

27. Preparation of oral solution of Rosyborella enterica live bacteria

Introduce the enteric Rosie barrett bacteria into the liquid seed medium, and cultivate it anaerobically at a suitable temperature to obtain the probiotic seed liquid. Put the probiotic seed solution into the sterilized intestinal Rosybacteria fermentation medium according to the total inoculation amount of 1-3%, then seal it with plastic film, let it stand for anaerobic cultivation, filter it, and aseptically infuse it To obtain the product, the number of viable bacteria is not less than 1.0*10 ⁸ CFU/ml. Example 3 Function experiment of probiotics regulating human intestinal flora

According to the Ministry of Health's "Health Food Inspection and Evaluation Technical Specifications (2003)", the evaluation method of the human body trial food experiment to regulate the function of intestinal flora is evaluated.

1. Grouping of feeding experiments

Table 1 Grouping table of eating experiment

2. Human food test method

The subjects were randomly divided into 27 groups, 10 people in each group. Before the subjects tried to eat, the feces of the subjects were collected aseptically, and the intestinal flora was tested by 16S rDNA sequencing as a reference for the background level.

According to the grouping situation in Table 1, group 1 takes composition 1, group 2 takes composition 2, group 3 takes composition 3, group 4 takes composition 4, group 5 takes composition 5, group 6 takes composition 6, group 7 taking composition 7, group 8 taking composition 8, group 9 taking composition 9, group 10 taking composition 10, group 11 taking composition 11, group 12 taking composition 12, group 13 taking composition 13, group 14 Taking composition 14, group 15 taking composition 15, group 16 taking composition 16, group 17 taking composition 17, group 18 taking composition 18, group 19 taking composition 19, group 20 taking composition 20, group 21 taking composition Composition 21, group 22 took composition 22, group 23 took composition 23, group 24 took composition 24, group 25 took composition 25, group 26 took composition 26, group 27 took composition 27, after 4 weeks, The feces of the subjects were collected, and the intestinal flora was tested by 16S rDNA sequencing. Compared with the background level, the relative abundance increase was compared.

3. Detection method of intestinal flora

Stool samples were first extracted from DNA, and the V3-V5 region of 16S rDNA was amplified by PCR, and then sequenced on the 454 platform, with the sequencing direction V5->V3. The raw data of each sample has an average of 7795 tags, and the read length is about 400bp. The average number of tags used for analysis is 3235, and the read length is about 265bp.

16S analysis mainly uses mothur software (http://www.mothur.org/wiki/Mothur_manual), including quality control, OTU clustering, annotation and other analysis, and on the basis of completing the data species classification, analyze the relative abundance of each species .

4. Experimental results

Table 2 Comparison of the abundance of sequencing data of each genera of human intestinal flora (compared with the background level before feeding)

Example 4 Probiotic composition lowering blood sugar, lowering blood fat experiment

According to the Ministry of Health's "Health Food Inspection and Evaluation Technical Specifications (2003)", the evaluation method of the auxiliary blood sugar and blood fat lowering function human body test food experiment evaluation method is carried out.

1. Preparation of probiotic composition:

According to Example 1, three different compositions of probiotic single powder (capsule), bacterial powder and polyfructose (capsule), bacterial powder and culture solution (oral solution) were prepared.

2. Human food trial and test method:

A total of 270 patients with type Ⅱ diabetes mellitus were selected on a voluntary basis, including 200 males and 70 females, aged 43-75 years, without serious complications such as heart, liver and kidney.

The subjects were randomly divided into 27 groups, with 10 people in each group. The types of therapeutic drugs, diet control and activities used by the subjects remained unchanged. According to the grouping situation in Table 1, Group 1 took Composition 1 and Group 2 took Composition 2. Group 3 took composition 3, group 4 took composition 4, group 5 took composition 5, group 6 took composition 6, group 7 took composition 7, group 8 took composition 8, group 9 took composition 9 , group 10 took composition 10, group 11 took composition 11, group 12 took composition 12, group 13 took composition 13, group 14 took composition 14, group 15 took composition 15, group 16 took composition 16, Group 17 took composition 17, group 18 took composition 18, group 19 took composition 19, group 20 took composition 20, group 21 took composition 21, group 22 took composition 22, group 23 took composition 23, group 24 took composition 24, group 25 took composition 25, group 26 took composition 26, and group 27 took composition 27, once a day, after meals, for 30 consecutive days.

Monitor the patient's blood pressure, urine and stool, and weight changes, and measure fasting and 2-hour postprandial blood glucose (using glucose oxidase method); measure blood lipids (total cholesterol TC, triglyceride TG and high-density lipoprotein HDL-C).

3. Judging criteria:

Significant effect: the basic symptoms disappear, and the blood sugar on an empty stomach or 2 hours after a meal drops by no more than 30.0% compared with that before treatment.

Effective: The basic symptoms are significantly improved, and the blood sugar on an empty stomach and 2 hours after a meal is less than 10.0% lower than that before treatment.

Ineffective: The basic symptoms did not improve significantly, and the blood sugar on an empty stomach and 2 hours after meals decreased by less than 10.0% compared with before treatment.

4. Human body test results:

After 30 days of food trial, the patients who took the probiotic composition of each group had no significant difference in blood pressure, urine and stool, and body weight compared with those before the food trial, but their fasting blood sugar and 2h postprandial blood sugar all decreased significantly. Among them, probiotics The combination of bacteria and their culture fluids had a more significant hypoglycemic effect, and the specific results are shown in Table 3.

Regarding the effect on blood lipids before and after the trial, the patients who took the probiotic composition of each group, compared with before the trial, the cholesterol and triglycerides of the patients after the trial for 30 days were significantly lower than those before the trial, and the high-density lipoprotein was lower than that before the trial. There was a significant increase before the test, but the combination of probiotics and their culture solution had a more significant hypoglycemic effect. The specific results are shown in Table 4.

It can be seen that the combination of probiotics and their culture fluids described in the present invention has obvious health care functions of assisting in lowering blood sugar and blood fat, and has no harmful effect on the health of the test population.

Table 3 Blood glucose test results of the trial population

Table 4 Blood lipid test results of the trial population

Note: (1) * means P<0.05; (2) ** means P<0.01

In the description of this specification, descriptions referring to the terms "one embodiment", "some embodiments", "example", "specific examples", or "some examples" mean that specific features described in connection with the embodiment or example , structure, material or characteristic is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be construed as limitations to the present invention. Variations, modifications, substitutions, and modifications to the above-described embodiments are possible within the scope of the present invention.

Claims (9)

1. An edible composition, characterized in that it comprises a probiotic culture, wherein the probiotic culture is obtained by cultivating the probiotic with a culture medium. 2. The edible composition according to claim 1, wherein the probiotic is selected from the group consisting of Faecalibacterium, Eubacterium, Roseburia, At least one of the genera Coprococcus, Bifidobacterium, Butyrivibrio, Bactobacillus, and Lactococcus. 3. The edible composition according to claim 2, wherein the probiotics are selected from the group consisting of Eubacterium rectal, Roseburia inulinivorans, Roseburia intestinalis Roseburia intestinalis, Faecalibacterium prausnitzii, Clostridium butyricum, Bacillus coagulans, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescent Bacillus, Lactobacillus delbrueckii sp. bulgaricus, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus casei subsp. casei, Lactobacillus reuteri, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii subspecies, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus salivarius, Propionibacterium freundii subsp. species, Lactococcus lactis subsp. cremoris, Lactococcus lactis (subsp. diacetyl), and Leuconostoc enteritidis subsp. enterica. 4. The edible composition according to claim 1, wherein the culture medium comprises carbon source, nitrogen source, growth factor, inorganic salt and water, in, The carbon source is at least one selected from sugars, organic acids, alcohols, and lipids, The nitrogen source is at least one selected from protein and its degradation products in varying degrees, ammonium salt, nitrate, and The growth factor is at least one selected from vitamins, amino acids, purine and pyrimidine bases. 5. The edible composition according to any one of claims 1-4, characterized in that, the edible composition is in the form of capsules or oral liquids. 6. A food, characterized in that it comprises the edible composition according to any one of claims 1-5. 7. The use of the edible composition according to any one of claims 1-5 in the preparation of preparations for at least one of regulating intestinal flora, lowering blood sugar, lowering blood fat and improving immunity. 8. Use of the edible composition according to any one of claims 1-5 or the food product according to claim 6 in regulating animal intestinal flora. 9. A method for regulating intestinal flora, characterized in that the edible composition according to any one of claims 1-5 or the food according to claim 6 is supplied to animals.