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CL2016002358A1 - Aumento de la expresión de proteína recombinante con cobre. - Google Patents

Aumento de la expresión de proteína recombinante con cobre.

Info

Publication number
CL2016002358A1
CL2016002358A1 CL2016002358A CL2016002358A CL2016002358A1 CL 2016002358 A1 CL2016002358 A1 CL 2016002358A1 CL 2016002358 A CL2016002358 A CL 2016002358A CL 2016002358 A CL2016002358 A CL 2016002358A CL 2016002358 A1 CL2016002358 A1 CL 2016002358A1
Authority
CL
Chile
Prior art keywords
copper
recombinant protein
increased expression
recombinant
factor viii
Prior art date
Application number
CL2016002358A
Other languages
English (en)
Inventor
Sadettin Seyit Ozturk
Matthew Veron Caple
Original Assignee
Advantech Bioscience Farmacêutica Ltda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advantech Bioscience Farmacêutica Ltda filed Critical Advantech Bioscience Farmacêutica Ltda
Publication of CL2016002358A1 publication Critical patent/CL2016002358A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

<p>LA PRESENTE INVENCIÓN PROPORCIONA UN USO INNOVADOR DE COBRE (ION CÚPRICO) PARA EXPRESIÓN CELULAR MEJORADA DE PROTEÍNAS RECOMBINANTES, EN PARTICULAR PROTEÍNAS DE COAGULACIÓN, TALES COMO FACTOR VIII RECOMBINANTE. FACTOR VIII RECOMBINANTE CON DOMINIO B EXCLUIDO. FACTOR IX RECOMBINANTE Y rFVII o rFVIIa. EL USO DE TAL SUPLEMENTO DE CULTIVO CELULAR RESULTA EN UNA MAYOR PRODUCTIVIDAD Y ROBUSTEZ DEL PROCESO DE MANUFACTURA. LA PRESENTE INVENCIÓN RESULTA EN MEJORAS DE LA EXPRESIÓN CELULAR YLA ESTABILIDAD DEL PRODUCTO.</p>
CL2016002358A 2014-03-23 2016-09-20 Aumento de la expresión de proteína recombinante con cobre. CL2016002358A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461969215P 2014-03-23 2014-03-23

Publications (1)

Publication Number Publication Date
CL2016002358A1 true CL2016002358A1 (es) 2017-07-07

Family

ID=54196507

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2016002358A CL2016002358A1 (es) 2014-03-23 2016-09-20 Aumento de la expresión de proteína recombinante con cobre.

Country Status (9)

Country Link
US (1) US20170067013A1 (es)
EP (1) EP3122770A4 (es)
KR (1) KR20160138477A (es)
CN (1) CN106459180A (es)
AU (1) AU2015234611A1 (es)
CA (1) CA2942770A1 (es)
CL (1) CL2016002358A1 (es)
MX (1) MX2016012428A (es)
WO (1) WO2015143512A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160138403A (ko) * 2014-02-17 2016-12-05 어드밴텍 바이오사이언스 파마큐티카 엘티디에이 막-기반의 세포 체류 시스템을 이용한 재조합 단백질의 발현을 증가시키는 방법
JP2017509659A (ja) 2014-04-01 2017-04-06 アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ 低糖−低グリシンの安定な第ｖｉｉｉ因子製剤
JP2017509658A (ja) 2014-04-01 2017-04-06 アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ カルシウム添加剤を使用しない第ｖｉｉｉ因子の安定化
AR104050A1 (es) * 2015-03-26 2017-06-21 Chugai Pharmaceutical Co Ltd Proceso de producción con iones de cobre controlados
WO2022125523A1 (en) * 2020-12-08 2022-06-16 Partner Therapeutics, Inc. Manufacture of granulocyte macrophage-colony stimulating factor

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO162160C (no) * 1987-01-09 1989-11-15 Medi Cult As Serumfritt vekstmedium, samt anvendelse derav.
US5804420A (en) * 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6200560B1 (en) * 1998-10-20 2001-03-13 Avigen, Inc. Adeno-associated virus vectors for expression of factor VIII by target cells
EP1233064A1 (en) * 2001-02-09 2002-08-21 Aventis Behring Gesellschaft mit beschränkter Haftung Modified factor VIII cDNA and its use for the production of factor VIII
AU2007269233B2 (en) * 2006-06-30 2011-06-09 Cnj Holdings, Inc. Method of producing Factor VIII proteins by recombinant methods
PL2115126T3 (pl) * 2007-03-02 2015-08-31 Wyeth Llc Zastosowanie miedzi i glutaminianu w hodowli komórkowej do wytwarzania polipeptydów
PL2501822T3 (pl) * 2009-11-17 2017-12-29 E. R. Squibb & Sons, L.L.C. Sposoby zwiększenia produkcji białek
US20130040888A1 (en) * 2010-02-16 2013-02-14 Novo Nordisk A/S Factor VIII Molecules With Reduced VWF Binding
US20140051832A1 (en) * 2011-03-11 2014-02-20 Universidade De São Paulo - Usp Method for the production of recombinant human factor viii

Also Published As

Publication number Publication date
CA2942770A1 (en) 2015-10-01
EP3122770A2 (en) 2017-02-01
MX2016012428A (es) 2017-04-27
EP3122770A4 (en) 2017-08-23
WO2015143512A2 (en) 2015-10-01
KR20160138477A (ko) 2016-12-05
AU2015234611A1 (en) 2016-11-10
WO2015143512A3 (en) 2015-12-10
CN106459180A (zh) 2017-02-22
US20170067013A1 (en) 2017-03-09

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