CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - - 1 2-PHENYL-3,4-DIHYDROPYRROLO[2,1 -F] [1,2,4]TRIAZINONE DERIVATIVES AS PHOSPHODIESTERASE INHIBITORS AND USES THEREOF The present invention relates to pharmaceutically useful compounds, in particular to compounds which are useful in the inhibition of cyclic guanosine 3',5'-monophosphate phosphodiesterases 5 (cGMP PDEs), and hereby in particular in the inhibition of type 5 cyclic guanosine 3',5'- monophosphate phosphodiesterase (cGMP PDE5). The compounds of the present invention have utility in a variety of therapeutic areas, including male erectile dysfunction (MED), Alzheimer’s disease, pulmonary artery hypertension (PAH), endothelial dysfunction (ED), benign prostatic hyperplasia (BPH) and lower urinaiy tract symptoms (LUTS), priapism, cystic fibrosis, 10 peripheral vascular disease, vascular disorders such as Raynaud's disease, systemic sclerosis (SSc), scleroderma, diabetes, and in particular for wound healing, in particular chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer and diabetic neuropathy. RELATED ART 15 Phosphodiesterases (PDEs) are enzymes that catalyzes the hydrolysis and thus the degradation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) and thereby regulates intracellular levels of second messengers. Inhibition of PDEs leads to increasing intracellular concentrations of endogenous cAMP/cGMP. Therefore, inhibition of PDE can mediate a variety of physiological mechanisms at different cell and organ levels. 20 Phosphodiesterase type 5 (PDE5) hydrolyses cyclic guanylate monophosphate (cGMP) specifically to 5' GMP. The selective inhibition of PDE5 has been validated as a relevant approach and strategies directed to promote inhibition of PDE5 activity have been applied as therapeutic tools, in particular, in neuronal and cardiovascular conditions. Moreover, the 25 introduction of PDE5 inhibitors has revolutionized the treatment of male erectile dysfunction (MED) (Dobhal T, Kaur S, Prakash Sharma O, Hari Kumar SL, Critical Review in Pharmaceutical Sciences (2012) 1(3):13-27). Several PDE5 inhibitors are on the market and are characterized particularly for MED or pulmonary hypertension (PH), in particular PAH (Papapetropoulos A, Hobbs AJ, Topouzis S, British Journal of Pharmacology (2015) 172:1397- 30 1414; Monica FZ, Murad F, Bian K, OA Biochemistry (2014) Mar 11; 2(1):3; Beedimani RS, Kalmath B, Int J Pharm Bio Sci (2014) 5(2): 530-539; Wronski S, Cent European J Urol (2014) 67: 314-318; and references cited therein). Most prominent examples of PDE5 inhibitors are CA 03001728 2018-04-11 _ _ WO 2017/085056 2 PCT/EP2016/077720 Sildenafil, Tadalafil and Vardenafil which have been described among others, for example, in WO 99/24433, WO 01/60825, EP 995’751 and WO 2011/075655. Beside the success of the known PDE5 inhibitors, there is still a need for further and in particular 5 more potent PDE5 inhibitors and their pharmaceutical compositions for use in the therapeutic treatment or prophylaxis of diseases associated with a disturbed cGMP balance. Moreover, and in general, there is still a need for compounds and their pharmaceutical compositions being beneficial for use in the therapeutic treatment or prophylaxis of diseases associated with a disturbed cGMP balance. 10 SUMMARY OF THE INVENTION We have surprisingly found that the compounds of the present invention are very potent and selective inhibitors of PDE5. Furthermore, we have surprisingly found that the compounds of the present invention can be tailored to become dual-pharmacology NO-releasing PDE5 inhibitors 15 which are believed to release NO in addition to its PDE 5 inhibition in a more than additive fashion. These dual-pharmacology NO-releasing PDE5 inhibitors are believed to be highly beneficial for the treatment of diabetic patients. Moreover, we have surprisingly found that preferred compounds of the present invention show even a significantly higher PDE5 inhibition activity as compared to known PDE5 inhibitors such as sildenafil. As a consequence, the novel 20 pyrrolo triazine compounds of the present invention are useful in the therapy and prophylaxis of diseases which are associated with a disturbed cGMP balance. Due to the potent and selective PDE5 inhibition exhibited by compounds of the present invention, cGMP levels are elevated, which in turn can give rise to beneficial vasodilatory, anti-vasospastic, anti-platelet, anti¬ neutrophil, natriuretic and diuretic activities. Furthermore, the tailoring of the inventive 25 compounds to dual-pharmacology NO-releasing PDE5 inhibitors allows the release of nitric oxide for activating the soluble guanylate cyclase as well as the PDE 5 inhibition in a more than additive fashion. Thus, the compounds of the present invention have utility in variety of therapeutic areas where a disturbed cGMP balance occurred and/or PDE5 inhibition is thought to be beneficial. Some of the preferred therapeutic areas are wound healing, in particular chronic 30 wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Raynaud’s, male erectile dysfunction, female sexual dysfunction, Alzheimer’s disease, diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension and chronic heart failure. CA 03001728 2018-04-11 _ _ WO 2017/085056 3 PCT/EP2016/077720 Thus, in a first aspect, the present invention provides for a compound of formula I R5 I or pharmaceutically acceptable salt, solvate or hydrate thereof, wherein 5 Ri is Cj-C3alkyl optionally substituted with F, C3-C6cycloalkyl, Ci-C3alkoxy; X represents a bond or Ci-C3alkylene optionally substituted with OH, ONO, ONO2; R2 is H, OH, ONO, ONO2, C(O)OH, C(O)OCi-C3alkyl, CHO, CN, C]-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylcnc-C(O)OH, OCi-C3alkylene-C(O)OCi- C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)Ci-C3alkyl, CR8=N-OR9, CR^N-NRwRn, 10 CR8=NRi2 or CR8=N-ONO2; R3 is Ci-Cgalkyl optionally substituted with F, OH, ONO, ONO2, Ci-C3alkoxy, C3-Cecycloalkyl; C3-C6cycloalkyl, C2-C6alkenyl, C2-C6alkynyl; R4 is Ci-Cealkyl optionally substituted with C3-Cecycloalkyl, Ci-Cealkoxy, F, ONO, ONO2; C2- C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl; 15 R5 is H, SO2NRi3Ri4, NHSO2NR13Rm; Re is H or Ci-C3alkyl; R7 is H, Ci-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl, F; 20 Rs is H, CH3 or C2H5; R9 is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi- C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)C]-C3alkyl; Rio and R„ are each independently H, C]-C3alkyl optionally substituted with OH, ONO, ONO2, 25 CN, COOH, COOCi-C3, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi- C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0. 2)Ci-C3alkyl;,or together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, CA 03001728 2018-04-11 _ _ WO 2017/085056 4 PCT/EP2016/077720 piperidine, morpholine, piperazine and homopiperazine, wherein said heterocyclic ring is optionally substituted with C1-C3 alkyl; R12 is C1-C3 alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-Csalkyl, Ci-C3alkoxy, OC(O)H, OC(O)-C]-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi- 5 C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(Oo-2)Ci-C3alkyl; R13 and Rm are each independently H or Ci-Cealkyl optionally substituted with F, OH, ONO, ONO2, COOH, Ci-Caalkoxy, Cs-Cecycloalkyl; or together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine, homopiperazine, 2,5- 10 diazabicyclo[2,2,l]heptane and 3,7-diazabicyclo[3,3,0]octane, wherein said heterocyclic ring is optionally substituted with R15; Ris is Ci-Cealkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-C^alkoxy, Ci- Qhaloalkoxy, COORm, NRi7R]8, C=NRi9, or with a tetrazole group which is optionally substituted with Cj-Csalkyl; or a heteroaryl ring which is optionally substituted with F, wherein 15 the at least one heteroatom of said heteroaryl ring is nitrogen; R16 is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2, NRnRis, or with a heteroaryl ring, wherein the at least one heteroatom of said heteroaryl ring is nitrogen, and wherein preferably said heteroaryl ring is selected from pyrrolidine, piperidine, piperazine, morpholine, pyrrole, and imidazole, wherein nitrogen atom is directly bound to C1-C4 alkyl; 20 Ri7 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO, ONO2; R19 is Ci-C4alkyl optionally substituted with F, ONO, ONO2; Ca-CecycloalkyL In a further aspect, the present invention provides for a pharmaceutical composition comprising at least one of the inventive compound of formula I, or a pharmaceutically acceptable salt, 25 solvate or hydrate thereof, and a pharmaceutically acceptable excipient, adjuvant, or carrier. In another aspect, the present invention provides for a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for use in a method of treating or preventing a disease alleviated by inhibition of PDE-5 in a 30 human or in a non-human mammal, preferably in a human. Preferably, said disease is selected from wound healing, chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Raynaud’s disease, male erectile dysfunction, female sexual dysfunction, diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension; stable, unstable and variant (Prinzmetal) angina; hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, CA 03001728 2018-04-11 WO 2017/085056 -5- PCT/EP2016/077720 congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, systemic sclerosis (SSc), scleroderma, morphea, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, diabetic neuropathy, Idiopathic pulmonary fibrosis (IPF), peyronic’s disease, glaucoma 5 or a disease characterized by disorders of gut motility like irritable bowel syndrome, liver fibrosis, Alzheimer’s disease and chronic heart failure, wherein further preferably said disease is selected from wound healing, chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, diabetic neuropathy, peripheral vascular disease, vascular disorders, Raynaud's disease, systemic sclerosis (SSc), scleroderma, diabetes, pulmonary artery hypertension, male erectile dysfunction, 10 and wherein again further preferably said disease is selected from wound healing, chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer and diabetic neuropathy. In again another aspect, the present invention provides for a compound of formula IV IV 15 wherein X, Ri, R2, R3, R4, R5 are defined as for the compound of formula I. In again another aspect, the present invention provides for a process for the preparation of a compound of formula I, x— r2 Rs I 20 wherein said process comprises: (a) reaction of a compound of formula II with a benzoic acid derivative of formula III in an aprotic or a protic solvent to generate a compound of formula IV CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 II III (b) cyclization of said compound of formula IV to yield compound of formula I, 5 wherein X, R], R2, R3, R4, and R5 are defined as for the compound of formula I. In again another aspect, the present invention provides for a process for the preparation of a compound of formula I, wherein said process comprises (a) reaction of a compound of formula VI with a benzoyl chloride derivative of formula VIA to generate a compound of formula VII 10 VI VIA VII (b) hydrolysis of the ester compound of formula VII to an acid derivative of formula VIII r5 VIII 15 (c) amination of said compound of formula VIII to yield a compound of formula IV CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 Rs IV (d) cyclization of said compound of formula IV to yield compound of formula I, wherein X, Ri, R2, R3, R4, and R5 are defined as for the compound of formula I; and wherein R’ is C1-C4 alkyl, benzyl, 4-alkoxybenzyl. 5 In again another aspect, the present invention provides for a process for the preparation of a compound of formula I, wherein said process comprises conversion of compound of formula IA to yield compound of formula I R5 IA wherein X, Ri, R2, R3, R4, and R5 are defined as for the compound of formula I in any one of the 10 claims 1 to 8. Further aspects and embodiments of the present invention will be become apparent as this description continues. 15 DESCRIPTION OF THE FIGURES FIG. 1: Concentration dependent measurements of cyclic guanosine 3’-5’-monophosphate (cGMP) in human pulmonary artery smooth muscle cells (hPASMC) incubated in presence of the compounds of the inventions or the reference PDE5 inhibitor sildenafil. CA 03001728 2018-04-11 _ _ WO 2017/085056 8 PCT/EP2016/077720 FIG. 1A: Concentration dependent measurement of cGMP in hPASMC incubated for 15 min in presence of inventive compound lr in concentrations of 1x10~16M (O.lfM) - 1xI07M (lOOnM). FIG. IB: Concentration dependent measurement of cGMP in hPASMC incubated for 30 5 min in presence of inventive compound Iv in concentrations of 1x10~12M (lpM) - 1x10~7M (lOOnM). FIG. IC: Concentration dependent measurement of cGMP in hPASMC incubated for 15 min in presence of reference PDE5 inhibitor sildenafil in concentrations of lxlO loM (O.lnM) - 1x10 7M (lOOnM). 10 FIG 2: Concentration dependent relaxation by Iv, lr, sildenafil of phenylephrine- precontracted rat aortic rings with intact endothelium (FIG. 2A), exposed to 25mM glucose for Ih (FIG. 2B), in presence of L-NAME (lOOpM) (FIG. 2C), following mechanical removal of intact endothelium (FIG. 2D). Results are depicted as the means ± SEM from 16-20 preparations. 15 FIG 3: Non-linear regression (Graph Pad Prism 7.01) of the concentration dependent relaxation by Iv, lr, sildenafil of phenylephrine-precontracted rat aortic rings with intact endothelium (FIG. 3A), exposed to 25mM glucose for Ih (FIG. 3B), in presence of L-NAME (lOOuM) (FIG. 3C), following mechanical removal of intact endothelium (FIG. 3D) depicted in FIG 2. Results are shown as the means ± SEM from 16-20 preparations. The stippled line 20 indicates the 40% inhibition level. DETAILED DESCRIPTION OF THE INVENTION Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art to which this invention 25 belongs. We have surprisingly found that the compounds of formula I of the present invention are very potent and selective inhibitors of PDE5. Furthermore, we have surprisingly found that the compounds of the present invention can be tailored to become dual-pharmacology NO-releasing 30 PDE5 inhibitors which are believed to release NO in addition to its PDE 5 inhibition in a more than additive fashion. Moreover, preferred compounds of the present invention show even a significantly higher PDE5 inhibition activity as compared to known PDE5 inhibitors such as sildenafil. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - - 9 Thus, in a first aspect, the present invention provides for a compound of formula I Rs I 5 or pharmaceutically acceptable salt, solvate or hydrate thereof, wherein Ri is Ci-C3alkyl optionally substituted with F, C3-C6cycloalkyl, Ci-C3alkoxy; X represents a bond or Ci-C3alkylene optionally substituted with OH, ONO, ONO2; R2 is H, OH, ONO, ONO2, C(O)OH, C(O)OCi-C3alkyl, CHO, CN, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi- 10 C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0.2)Ci-C3alkyl, CR8=N-OR9, CRS N-NRmRi i, CRs NR|2 or CRs=N-ONO2; R3 is C ।-Chalky optionally1 substituted with F, OH, ONO, ONO2, Ci-C3alkoxy, C3-Cecycloalkyl; Q-Cecycloalkyl, C2-Cealkenyl, C2-Cealkynyl; R4 is Ci-C6alkyl optionally substituted with C3-C6cycloalkyl, C]-C6alkoxy, F, ONO, ONO2; C2- 15 Cgalkenyl, C2-C6alkynyl, C3-C6cycloalkyl; R5 is H, SO2NRi3Ri4, NHSO2NRi3R]4; Re is H or C]-C3alkyl; R- is H, Ci-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally 20 substituted by Ci-C3alkyl, F; Rs is H, CH3 or C2H5; R9 is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi- C3alkylenc-C(O)OCi-C3alkyl, OCi-C3alkylcnc-C(O)N(R6)OR7, S(O0.2)Ci-C3alkyl; 25 Rio and Rn are each independently H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOC1-C3, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi- C3alkylene-C(O)OH, OC1-C3alkylene-C(O)OC1-C3alkyl, OC,-Cialkylcnc-C(O)N(R6)OR-, S(O0_ 2)Ci-C3alkyl;jor together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 10 - piperidine, morpholine, piperazine and homopiperazine, wherein said heterocyclic ring is optionally substituted with C1-C3 alkyl; R12 is C1-C3 alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-Csalkyl, Ci-C3alkoxy, OC(O)H, OC(O)-C]-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi- 5 C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(Oo-2)Ci-C3alkyl; R13 and Rm are each independently H or Ci-Cealkyl optionally substituted with F, OH, ONO, ONO2, COOH, Ci-Caalkoxy, Cs-Cecycloalkyl; or together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine, homopiperazine, 2,5- 10 diazabicyclo[2,2,l]heptane and 3,7-diazabicyclo[3,3,0]octane, wherein said heterocyclic ring is optionally substituted with R15; Ris is Ci-Cealkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-C^alkoxy, Ci- Qhaloalkoxy, COORm, NRi7R]8, C=NRi9, or with a tetrazole group which is optionally substituted with Ci-Csalkyl; or a heteroaryl ring which is optionally substituted with F, wherein 15 the at least one heteroatom of said heteroaryl ring is nitrogen; R16 is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2, NRnRis, or with a heteroaryl ring, wherein the at least one heteroatom of said heteroaryl ring is nitrogen, and wherein preferably said heteroaryl ring is selected from pyrrolidine, piperidine, piperazine, morpholine, pyrrole, and imidazole, wherein nitrogen atom is directly bound to C1-C4 alkyl; 20 Ri7 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO, ONO2; R19 is Ci-C4alkyl optionally substituted with F, ONO, ONO2; Ca-CecycloalkyL Thus, in a further aspect, the present invention provides for a compound of formula I 25 or pharmaceutically acceptable salt, solvate or hydrate thereof, wherein Ri is Ci-C;alkyl optionally substituted with F, Cs-Cecycloalkyl, Ci-Csalkoxy; X represents a bond or Ci-Cjalkylcnc optionally substituted with OH, ONO, ONO2; CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 11 - R2 is OH, ONO, ONO2, C(O)OH, C(O)OCi-C3alkyl, CHO, CN, Ci-C3alkoxy, OC(O)H, OC(O)- Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi- C3alkylene-C(O)N(R«)OR7, S(O0-2)Ci-C3alkyl, CR8=N-OR9, CR8=N-NRi0Rn, CR8=NRi2 or CRs=N-ONO2; 5 R3 is Ci-Cgalkyl optionally substituted with F, OH, ONO, ONO2, Ci-C3alkoxy, Q-Cecycloalkyl; C3-C6cycloalkyl, C2-C6alkenyl, C2-C6alkynyl; R4 is Ci-Cealkyl optionally substituted with C3-Cecycloalkyl, C|-C6alkoxy, F, ONO, ONO2; C2- C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl; R5 is H, SO2NRi3Rm, NHSO2NR|3Rh; 10 R^ is H or Ci-C3alkyl; R7 is H, Ci-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl, F; R8 is H, CH3 or C2H5; 15 R9 is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCr C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)Ci-C3alkyl; Rio and Rn are each independently H, C]-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi- 20 C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0. 2)Ci-C3alkyl;,or together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine and homopiperazine, wherein said heterocyclic ring is optionally substituted with Ci-C3 alkyl; 25 Ri2 is Ci-C3 alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OC’i- C3alkylene-C(O)OCj-C3alkyl, OC]-C3alkylene-C(O)N(R6)OR7, S(O0-2)C]-C3alkyl; Ri3 and R14 are each independently H or Ci-Cealkyl optionally substituted with F, OH, ONO, ONO2, COOH, Ci-C3alkoxy, C3-Cecycloalkyl; or together with the nitrogen atom to which they 30 are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine, homopiperazine, 2,5- diazabicyclo[2,2,l]heptane and 3,7-diazabicyclo[3,3,0]octane, wherein said heterocyclic ring is optionally substituted with Ri5; CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 12 - Ris is Ci-Cealkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-C3alkoxy, Ci- C3haloalkoxy, COORu, NR17R18, C=NRi9, or with a tetrazole group which is optionally substituted with Ci-C3alkyl; or a heteroaryl ring which is optionally substituted with F, wherein the at least one heteroatom of said heteroaryl ring is nitrogen; 5 Rie is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2, NR17R18, or with a heteroaryl ring, wherein the at least one heteroatom of said heteroaryl ring is nitrogen, and wherein preferably said heteroaryl ring is selected from pyrrolidine, piperidine, piperazine, morpholine, pyrrole, and imidazole, wherein nitrogen atom is directly bound to C1-C4 alkyl; R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO, ONO2; 10 R19 is Ci-C4alkyl optionally substituted with F, ONO, ONO2; C3-C6cycloalkyl. Each alkyl moiety either alone or as part of a larger group such as alkoxy or alkylene is a straight or branched chain and is preferably Ci-Cgalkyl, more preferably Ci-CjalkyL Examples include methyl, ethyl, n-propyl, prop-2-yl, «-butyl, but-2-yl, 2-methyl-prop-l-yl or 2-methyl-prop-2-yl. 15 Examples of an alkoxy include methoxy, ethoxy, propoxy, zso-propoxy, n-butoxy, .sec-butoxy, tert-butoxy, zz-pentoxy, neo-pentoxy, zz-hexoxy. As described herein, alkoxy may include further substitutents such as halogen atoms leading to haloalkoxy moieties. Each alkylene moiety is a straight or branched chain and is, for example, -CH2-, -CH2-CH2-, - 20 CH(CH3)-, -CH2-CH2-CH2-, -CH(CH3)-CH2-, or -CH(CH2CH3)-. Each cycloalkyl moiety can be in mono- or bi-cyclic form, typically and preferably in mono- cyclic form, and preferably contains 3 to 8 carbon atoms, more preferably 3 to 7 carbon atoms. Examples of monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl and cyclohexyl. 25 Each alkenyl moiety either alone or as part of a larger group such as alkenyloxy or alkenylene is a straight or branched chain and is preferably C2-Cgalkenyl, more preferably C2-C4alkenyl. Each moiety can be of either the (E)- or (.^-configuration. Examples include vinyl and allyl. A compound of the present invention comprising an alkenyl moiety thus may include, if applicable, 30 either said compound with said alkenyl moiety in its (E')-configuration, said compound with said alkenyl moiety in its (Z)-configuration and mixtures thereof in any ratio. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 13 - Each alkynyl moiety either alone or as part of a larger group such as alkynyloxy is a straight or branched chain and is preferably Cz-Cgalkynyl, more preferably C2-C4alkynyl. Examples are ethynyl and propargyl. 5 Halogen is fluorine, chlorine, bromine, or iodine. Each haloalkyl moiety either alone or as part of a larger group such as haloalkoxy is an alkyl group substituted by one or more of the same or different halogen atoms. Examples include difluoromethyl, trifluoromethyl, chlorodifluoromethyl and 2,2,2-trifluoro-ethyl. 10 The term "heterocyclic ring" refers to a saturated or partially unsaturated carbocyclic ring containing one to four heteroatoms selected from nitrogen, oxygen and sulfur as ring members. Such rings do not contain adjacent oxygen atoms, adjacent sulfur atoms, or adjacent oxygen and sulfur atoms within the ring. Preferred examples are aziridine, azetidine, pyrollidine, piperidine, 15 morpholine, piperazine, homopiperazine, tetrahydrofurane, dioxane, 2,5- diazabicyclo[2,2,l]heptane and 3,7-diazabicyclo[3,3,0]octane, and further preferred are aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine, homopiperazine, 2,5- diazabicyclo[2,2,1]heptane and 3,7-diazabicyclo[3,3,0]octane. 20 The term "heteroaryl" refers to an aromatic ring system containing at least one heteroatom, and preferably up to three heteroatoms selected from nitrogen, oxygen and sulfur as ring members. Heteroaryl rings do not contain adjacent oxygen atoms, adjacent sulfur atoms, or adjacent oxygen and sulfur atoms within the ring. Preferred examples are include pyrrolidine, piperidine, piperazine, morpholine, pyridine, pyrimidine, pyrazine, pyridazine, pyrrole, pyrazole, imidazole, 25 triazole, isoxazole, oxazole, isothiazole, thiazole, tetrazole, furane, and thiophenyl, and further preferred are pyrrolidine, piperidine, piperazine, morpholine, pyrrole, and imidazole. Where a group is said to be optionally substituted, preferably there are optionally 1-5 substituents, more preferably optionally 1-3 substituents, again more preferably optionally 1 or 2 30 substituents. Where a group is said to be optionally substituted, and where there are more than one substituents for said optional substitution of said group, said more than one substituents can either be the same or different. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 Certain compounds of formula I of the present invention may contain one or two or more centers of chirality and such compounds may be provided as pure enantiomers or pure diastereoisomers as well as mixtures thereof in any ratio. The compounds of the invention also include all tautomeric forms of the compounds of formula I. The compounds of formula I may also be 5 solvated, especially hydrated, which are also included in the compounds of formula I. Solvation and hydration may take place during the preparation process. As a consequence, the compounds of the present invention and, thus, the compounds of formula I include stereoisomers, geometric isomers and tautomers. Furthermore, the compounds of the 10 present invention and, thus, the compounds of formula I include solvates or hydrates, pharmaceutically acceptable salts, and solvates or hydrates of the salts thereof. Compounds of formula I of the present invention include pharmaceutically acceptable salts of said compounds. In particular, the term "pharmaceutically acceptable salt" as used herein, refers 15 to pharmaceutically acceptable organic or inorganic salts of a compound of the present invention, in particular acid addition salts. Exemplary salts include, but are not limited to, salts of physiologically acceptable mineral acids, such as hydrochloric acid, sulfuric acid, nitric acid and phosphoric acid, or salts of organic acids, such as methane-sulfonic acid, /?-toluenesulfonic acid, lactic acid, malic acid, tartaric acid, acetic acid, trifluoroacetic acid, citric acid, succinic 20 acid, fumaric acid, maleic acid and salicylic acid. Further examples of pharmacologically acceptable salts of the compounds of formula I are alkali metal and alkaline earth metal salts such as, for example, sodium, potassium, lithium, calcium or magnesium salts, ammonium salts or salts of organic bases such as, for example, methylamine, dimethylamine, triethylamine, piperidine, ethylenediamine, lysine, choline hydroxide, meglumine, morpholine or arginine salts. 25 Further examples of pharmaceutically acceptable salts of the compounds of formula I include the hydrochloride, hydrobromide, sulfate, bisulfate, phosphate, hydrogen phosphate, nitrate, acetate, benzoate, succinate, fumarate, maleate, lactate, citrate, benzenesulphonate, p-toluenesulphonate or the like. 30 A "solvate" refers to an association or complex of one or more solvent molecules and a compound of the present invention. Examples of solvents that form solvates include, but are not limited to, water, isopropanol, ethanol, methanol, dimethyl sulfoxide (DMSO), ethyl acetate, acetic acid, and ethanolamine. The term "hydrate" refers to the complex where the solvent molecule is water. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 15 - In a preferred embodiment of the present invention, Ri is Ci-C3alkyl. In a further preferred embodiment, Ri is CH3 or C2H5, and again further preferably Ri is CH3. 5 In another preferred embodiment, R3 is Ci-Cealkyl optionally substituted with F, OH, ONO, ONO2, Ci-C3alkoxy, C3-C6cycloalkyl, C2-C4alkenyl. In a further preferred embodiment, R3 is Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2, Ci-C3alkoxy, C3-C6cycloalkyl, C2- C4alkenyl. In a further preferred embodiment, R3 is Ci-C4alkyl optionally substituted with ONO, ONO2 or Ca-Cscycloalkyl. In a further preferred embodiment, R3 is Ci-C4alkyl optionally 10 substituted with ONO2 or C3-C5cycloalkyl. In a further preferred embodiment, R3 is Ci-Cealkyl, preferably R3 is Ci-C4alkyl. In a very preferred embodiment, R3 is n-propyl. In another preferred embodiment, R4 is Ci-C4alkyl optionally substituted with C3-C6cycloalkyl, Ci-Cealkoxy, F, ONO, ONO2; C2-C4alkenyl. In a further preferred embodiment, R4 is Ci-C4alkyl 15 optionally substituted with ONO, ONO2, Cj-Cealkoxy, or C3-C5cycloalkyl. In a further preferred embodiment, R4 is Ci-C4alkyl optionally substituted with ONO, ONO2 or Ci-Cealkoxy. In a further preferred embodiment, R4 is Ci-C4alkyl optionally substituted with ONO2 or Ci- Cealkoxy. In a further preferred embodiment, R4 represents Ci-Cealkyl, preferably Cj-C4alky, again preferably R4 represents ethyl or w-propyl. 20 In a further preferred embodiment, said Ri is Ci-C3alkyl; R3 is Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2, C]-C3alkoxy, C3-Cecycloalkyl, C2-C4alkenyl; and R4 is Ci-C4alkyl optionally substituted with C3-Cecycloalkyl, C]-C3alkoxy, F, ONO, ONO2; C2-C4alkenyl. 25 In a further preferred embodiment, said Ri is CH3 or C2H5, preferably Ri is CH3; R3 is Ci- C4alkyl optionally substituted with ONO2 or C3-Cscycloalkyl, preferably R3 is n-propyl; and R4 is Ci-C4alkyl optionally substituted with ONO2 or C3-C5cycloalkyl, preferably R4 is ethyl or n- propyl. 30 In a further preferred embodiment, said R]3 and R14 together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine, homopiperazine, 2,5- diazabicyclo-[2,2,l]heptane and 3,7-diazabicyclo[3,3,0]octane, wherein said heterocyclic ring is optionally substituted with R15; R15 is Ci-C4alkyl optionally substituted with halogen, OH, ONO, CA 03001728 2018-04-11 WO 2017/085056 -16- PCT/EP2016/077720 ONO2, Ci-Csalkoxy, Ci-Cshaloalkoxy, COORm, NR17R18, C=NRi9; Rie is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2; R17 and Ris are each independently H or Ci- C4alkyl optionally substituted with ONO, ONO2, preferably R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO2; R19 is Ci-C4alkyl optionally substituted with 5 F, ONO, ONO2, preferably R19 is Ci-C4alkyl optionally substituted with ONO2. In a further preferred embodiment, said R5 is SO2NR13R14, NHSO2NR13R14; said R13 and R14 together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, 10 piperazine, homopiperazine, 2,5- diazabicyclo-[2,2,l]heptane and 3,7-diazabicyclo[3,3,0]octane, wherein said heterocyclic ring is optionally substituted with Ris; said R15 is Ci-C4alkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-Csalkoxy, COORie, NR17R18, C=NRi9; R]6 is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2; said R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO, ONO2; said R19 is 15 Ci-C4alkyl optionally substituted with F, ONO, ONO2. In a further preferred embodiment, said Rs is SO2NR13R14, wherein R13 and R14 are each independently H or together with the nitrogen atom to which they are attached form a mono- cyclic ring selected from imidazol, aziridine, azetidine, pyrrolidine, piperidine, morpholine, 20 piperazine and homopiperazine optionally substituted with R15; said R15 is Ci-C4alkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-Csalkoxy, COORm, NR17R18, C=NRi9; Rie is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2; said R17 and Rm are each independently H or Ci-C4alkyl optionally substituted with ONO, ONO2; said R19 is Ci-C4alkyl optionally substituted with F, ONO, ONO2. 25 In a further preferred embodiment, said Rs is SO2NR13R14, wherein R13 and Rm together with the nitrogen atom to which they are attached form a mono-cyclic ring selected from imidazol, aziridine, azetidine, pyrrolidine, piperidine, morpholine, piperazine and homopiperazine optionally substituted with Ri5; said R15 is C]-C4alkyl optionally substituted with halogen, OH, 30 ONO, ONO2, Ci-C3alkoxy, COORm, NR17R18, C-NRI9; Rm is H, or Ci-C4alkyl optionally substituted with OH, ONO, ONO2; said R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO, ONO2; said R19 is C]-C4alkyl optionally substituted with ONO, ONO2. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 17 - In a further preferred embodiment, said Rs is SO2NR13R14, wherein Ru and R14 together with the nitrogen atom to which they are attached form a mono-cyclic ring selected from imidazol, aziridine, azetidine, pyrrolidine, piperidine, morpholine, piperazine and homopiperazine optionally substituted with R15; said R15 is Cj-C4alkyl optionally substituted with halogen, OH, 5 ONO2, Ci-C3alkoxy, COORie, NR17R18, C=NRi9; Rie is H, or Ci-C4alkyl optionally substituted with OH, ONO2; said R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO2; said R19 is Ci-C4alkyl optionally substituted with ONO2. In a further preferred embodiment, said R5 is SO2NR13R14, wherein R13 and R14 together with the 10 nitrogen atom to which they are attached form a mono-cyclic ring selected from imidazol, pyrrolidine, piperidine, morpholine, piperazine and homopiperazine optionally substituted with Ris; said Ris is Ci-C4alkyl optionally substituted with OH, ONO2, Ci-C3alkoxy, COORie, NR17R18, C=NRi9; Rie is H, or Ci-C4alkyl optionally substituted with OH, ONO2; said R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with ONO2; said R19 is Ci- 15 C4alkyl optionally substituted with ONO2. In a further very preferred embodiment, said R5 is SO2NR13R14, wherein R13 and R14 together with the nitrogen atom to which they are attached form a mono-cyclic ring selected from piperidine, and piperazine optionally substituted with RiS; said R15 is Ci-C3alkyl optionally 20 substituted with OH or ONO2. In a further preferred embodiment, said X represents a bond or C]-C3alkylene optionally substituted with OH. In a further very preferred embodiment, said X represents a bond or Ci- C2alkylene optionally substituted with OH. In another very preferred embodiment, said X 25 represents a bond. In another very preferred embodiment, said X represents Ci-C2alkylene optionally substituted with OH. In a further preferred embodiment, said R2 is H, OH, C(O)OH, C(O)OCi-C3alkyl, CHO, CN, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OC1-C3alkylene-C(O)OH, OCi-C3alkylene- 30 C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)Ci-C3alkyl, CH=N-OR9, wherein R9 is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci- C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi- C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(Oo-2)Ci-C3alkyl. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 18 - In a further preferred embodiment, said R2 is H, OH, C(O)OH, C(O)OCi-C3alkyl, CHO, CN, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene- C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)Ci-C3alkyl, CH=N-OR9, wherein R7 is H, Cj-C3alkyl; and wherein Rq is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, 5 CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(Rs)OR7, OCi- C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OC]-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0. 2)C1-C3alkyl. In a further preferred embodiment, said R2 is C(O)OH, C(O)OC]-C3alkyl, CHO, CN, OC(O)H, 10 OC(O)-Ci-C3alkyl, C(O)N(R«)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi- C3alkyl, OCi-C3alkylene-C(O)N(Re)OR7, S(Oo-2)Ci-C3alkyl, CH=N-OR9, wherein R7 is H, Ci- C3alkyl; and wherein R9 is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-C]-C3alkyl, C(O)N(R6)OR7, OCi- C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0_ 15 2)Ci-C3alkyl. In a further preferred embodiment, said R2 is C(O)OH, C(O)OCi-C3alkyl, CHO, CN, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi- C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0_2)Ci-C3alkyl, CH=N-OR9, wherein R7 is H, Ci- 20 C3alkyl; and wherein R9 is H, Cj-C3alkyl substituted with OH, ONO, ONO2, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6>OR7, OCi-C3alkylene- C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0.2)Ci- C3alkyl. 25 In a further very preferred embodiment, said R2 is CHO, CN, CH=N-OR9, preferably (£)-CH=N- OR9, wherein R9 is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOC]-C3alkyl, OC(O)H, OC(O)-C]-C3alkyl. In a further very preferred embodiment, said R2 is CHO, CN, CH=N-OR9, preferably (E)-CH=N- 30 OR9, wherein R9 is H, Ci-C3alkyl substituted with OH, ONO, ONO2, CN, COOH, COOCi- C3alkyl, OC(O)H, OC(O)-Ci-C3alkyl. In a further very preferred embodiment, said R2 is CH=N-OR9, preferably ('£)-CH=N-OR9. wherein R9is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi- CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 19 - C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)Ci-C3alkyl. In a further very preferred embodiment, said R2 is CH=N-OR9, preferably (E)-CH=N-ORy. 5 wherein R9is H, Ci-C3alkyl optionally substituted with OH, ONO, ONO2, CN, COOH, COOCi- C3alkyl, OC(O)H, OC(O)-C]-C3alkyl. In a further very preferred embodiment, said X-R2 represents Ci-C3alkylene optionally substituted with OH, ONO, ONO2, CN, C(O)OH, Ci-C2alkoxy, C(O)OCi-C3alkyl, 10 C(O)N(R6)OR7, CHO, OC(O)H, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)N(R6)OR7, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, CRs=N-OR9; C(O)OC]-C3alkyl, CHO, C(O)N(R6)OR7, S(Oo-2)Ci-C3alkyl, CR8=N-OR9, CRs=N-NRioRi 1, CR8=NRi2 or CR8=N-ONO2, wherein further preferably, said R6 is H or CH3, and said R8 is H or CH3. 15 In a further very preferred embodiment, said X-R2 represents Ci-C3alkylene substituted with OH, ONO, ONO2, CN, C(O)OH, Ci-C2alkoxy, C(O)OCi-C3alkyl, C(O)N(R6)OR7, CHO, OC(O)H, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)N(R6)OR7, OC(O)-Ci-C3alkyl, C(O)N(Rs)OR7, CR8=N-OR9; C(O)OCi-C3alkyl, CHO, C(O)N(R6)OR7, S(O0.2)Ci-C3alkyl, CRs=N-OR9, CR8=N- NR10R11, CR8=NRi2 or CRs=N-ONO2, wherein further preferably, said Re is H or CH3, and said 20 Rs is H or CH3. In a further preferred embodiment, R7 is H, C]-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine, homopiperazine, 2,5- diazabicyclo[2,2,l]heptane and 3,7- 25 diazabicyclo[3,3,0]octane, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl, F. In a further preferred embodiment, R7 is H, C]-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring selected from pyrollidine, piperidine, morpholine, piperazine, homopiperazine, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl. 30 In a further preferred embodiment, said R9 is H, Ci-C3alkyl substituted with OH, CN, COOH, COOC]-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene- C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0-2)Ci- C3alkyl. In a further preferred embodiment, said R9 is H, Ci-C3alkyl substituted with OH, CN, CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -20 - COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi- C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7. In a further preferred embodiment, said R]0 and Rn are each independently H, C]-C3alkyl 5 optionally substituted with OH, CN, COOH, COOCi-C3, Ci-C3alkoxy, OC(O)H, OC(O)-Ci- C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCj- C3alkylene-C(0)N(Ro)OR7, S(Oo-2)Ci-C3alkyl;i or together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from aziridine, azetidine, pyrollidine, piperidine, morpholine, piperazine and homopiperazine, wherein 10 said heterocyclic ring is optionally substituted with Ci-C3 alkyl. In a further preferred embodiment, said Rio and Rn are each independently H, Ci-C3alkyl optionally substituted with OH, CN, COOH, COOCi-C3, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(0)N(Ro)OR7, OCi- C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OC]-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, S(O0. 2)Ci-C3alkyl;>or together with the nitrogen atom to which they are attached form a heterocyclic 15 ring, wherein preferably said heterocyclic ring is selected from pyrollidine, piperidine, morpholine, piperazine and homopiperazine, wherein said heterocyclic ring is optionally substituted with Ci-C3 alkyl. In a further very preferred embodiment, said X-R2 represents Ci-C3alkylene substituted with CN, 20 C(O)OH, C(O)OCi-C3alkyl, C(0)N(Ro)OR7, CHO, OC(O)H, OCi-C3alkylene-C(O)OH, OCi- C3alkylene-C(O)N(R6)OR7, OC(O)-Ci-C3alkyl, C(O)N(Ro)OR7, CR8=N-OR9; C(O)OCi-C3alkyl, CHO, C(O)N(R6)OR7, S(O0-2)Ci-C3alkyl, CRs=N-OR9, CR8=N-NRi0Rn, CR8=NRi2 or CR8=N- ONO2, wherein further preferably, said Ro is H or CH3; said R7 is H, Ci-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring selected from pyrollidine, 25 piperidine, morpholine, piperazine, homopiperazine, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl; said R8 is H or CH3; said R9 is H, Ci-C3alkyl substituted with OH, CN, COOH, COOC]-C3alkyl, C]-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(Ro)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene- C(O)OCi -C3alkyl, OC]-C3alkylene-C(0)N(Ro)OR7. 30 In a further very preferred embodiment, said X-R2 represents Ci-C3alkylene substituted with CN, C(O)OH, C(O)OCi-C3alkyl, C(0)N(Ro)OR7, CHO, OC(O)H, OCi-C3alkylene-C(O)OH, OCi- C3alkylene-C(0)N(Ro)OR7, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, CR8=N-OR9; C(O)OCi-C3alkyl, CHO, C(0)N(Ro)OR7, S(O0-2)Ci-C3alkyl, CR8=N-OR9, CR8=N-NRI0Rn, wherein further CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -21 - preferably, said Re is H or CH3; said R7 is H, Ci-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring selected from pyrollidine, piperidine, morpholine, piperazine, homopiperazine, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl; said Rg is H or CH3; said R9 is H, Ci-C3alkyl 5 substituted with OH, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OC]-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene- C(O)N(Re)OR7; said Rio and Rn are each independently H, Ci-C3alkyl optionally substituted with OH, CN, COOH, COOCi-C3, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi-C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(R6)OR7, 10 S(Oo-2)Ci-C3alkyl;, or together with the nitrogen atom to which they are attached form a heterocyclic ring, wherein preferably said heterocyclic ring is selected from pyrollidine, piperidine, morpholine, piperazine and homopiperazine, wherein said heterocyclic ring is optionally substituted with C]-C3 alkyl. 15 In a further very preferred embodiment, said X-R2 represents Ci-C3alkylene substituted with CN, C(O)OH, C(O)OCi-C3alkyl, C(O)N(R6)OR7, CHO, OC(O)H, OCi-C3alkylene-C(O)OH, OCi- C3alkylene-C(O)N(R<;)OR7, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, CR8=N-OR9; C(O)OCi-C3alkyl, CHO, C(O)N(Re)OR7, CRg=N-OR9, wherein further preferably, said Re is H or CH3; said R7 is H, Ci-C3alkyl, Ci-C3alkoxy, Ci-C3alkyl substituted with phenyl, benzyl or a heterocyclic ring 20 selected from pyrollidine, piperidine, morpholine, piperazine, homopiperazine, wherein said phenyl, benzyl or said heterocyclic ring are independently optionally substituted by Ci-C3alkyl; said R8 is H or CH3; said R9 is H, Ci-C3alkyl substituted with OH, CN, COOH, COOCi-C3alkyl, Ci-C3alkoxy, OC(O)H, OC(O)-Ci-C3alkyl, C(O)N(R6)OR7, OCi-C3alkylene-C(O)OH, OCi- C3alkylene-C(O)OCi-C3alkyl, OCi-C3alkylene-C(O)N(Re)OR7. 25 In a further very preferred embodiment, said X-R2 represents CR8=N-OR9, preferably (E)- CR8=N-OR9> wherein said R8 is H or CH3, preferably wherein said Rg is H, and wherein said R9 is H or Ci-C3 alkyl optionally substituted with OH or Ci-C3 alkyl optionally substituted with OH. 30 In a further very preferred embodiment, said X-R2 represents CH=N-OR9, preferably (£)-CH=N- OR9, wherein said R9 is H or Ci-C3 alkyl optionally substituted with OH or CN, and wherein preferably said R9 is H or Ci-C3 alkyl substituted with OH or CN; and wherein further preferably said R9 is H or Ci-C3 alkyl substituted with OH. CA 03001728 2018-04-11 WO 2017/085056 -H- PCT/EP2016/077720 Without being bound this theory, it is believed that the functionalization of the group R2 and thus the group X-R2, allows an increased interaction with the PDE5 enzyme and thus an increased inhibition effect. In particular, R2 with an oxime functionality, and hereby in particular with a trans-geometry of the oxime functionality has been found to be highly beneficial. 5 In a further preferred embodiment, said Ri is C1-C3 alkyl; said R2 is CH=N-OR<j; preferably (E)- CH=N-OR9, wherein said Ry is H or C1-C3 alkyl optionally substituted with OH or C1-C3 alkyl optionally substituted with OH; R3 is Ci-Cg alkyl; said R4 is C1-C6 alkoxy; said R5 is SO2NRi3Ri4 wherein R13 and R14 are each independently H or together with the nitrogen atom to which they 10 are attached form a mono-cyclic ring selected from imidazol, aziridine, azetidine, pyrrolidine, piperidine, morpholine, piperazine and homopiperazine optionally substituted with R15; wherein said R15 is Ci-C4alkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-C3alkoxy, COOR16, NR17R18, C=NRi9; Rie is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2; said R17 and Ris are each independently H or Ci-C4alkyl optionally substituted with 15 ONO, ONO2; said R19 is Ci-C4alkyl optionally substituted with F, ONO, ONO2. In a further preferred embodiment, said Ri is C1-C3 alkyl; said R2 is CH=N-OR9; preferably (E)- CH=N-OR9, wherein said R9 is H or C1-C3 alkyl optionally substituted with OH or CN; R3 is Ci- Ce alkyl; said R4 is Ci-Ce alkoxy; said Rs is SO2NRi3Ri4 wherein R13 and Rn are each 20 independently H or together with the nitrogen atom to which they are attached form a mono- cyclic ring selected from imidazol, aziridine, azetidine, pyrrolidine, piperidine, morpholine, piperazine and homopiperazine optionally substituted with R15; wherein said R15 is Ci-C4alkyl optionally substituted with halogen, OH, ONO, ONO2, Ci-Csalkoxy, COORie, NRnRis, C=NRi9; Rie is H, or Ci-C4alkyl optionally substituted with F, OH, ONO, ONO2; said R17 and 25 Ris are each independently H or C]-C4alkyl optionally substituted with ONO, ONO2; said R19 is Ci-C4alkyl optionally substituted with F, ONO, ONO2. Further very preferred embodiments of the present invention are represented by individual compounds of formula I or pharmaceutically acceptable salts, solvates or hydrates thereof. 30 Thus, in another very preferred embodiment, said compound of formula I is selected from (£)-2-(2-cthoxy-5-((4-mcthylpipcrazin-l-yl)sulfonyl)phcnyl)-5-mcthyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde oxime WO 2017/085056 -23- PCT/EP2016/077720 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-6-(hydroxymethyl)-5-methyl-7- propylpyrrolo[2,l-f][l,2,4]triazin-4(3H)-one 5 (2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-nietliyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][l ,2,4]triazin-6-yl)methyl acetate (1c); 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][l ,2,4]triazine-6-carboxylic acid 10 (Id); N-(benzyloxy)-2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carboxamide Methyl 2-(2-ethoxy-5-((4-methylpiperazin- -yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-31 ,4- 15 dihydropyrrolo[2,1-f][1,2,4]triazine-6-carboxylate dihydropyrrolo[2, WO 1 2017/085056 -f] [ 1Z-((Z-(Z-ethoxy-D-((4-methylpiperazm- _ 1 _ ,2,4]triazin-6-yl)methoxy)-N-hydroxy-N-methylacetamide -yi)sultonyl)phenyl)-D-methyl-4-oxo- 74 /-propyl-. PCT/EP2016/077720 CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 6-(l,3-dihydroxypropyl)-2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl) phenyl)-5-methyl-7- propylpyrrolo[2, 1-f][1,2,4]triazin-4(3H)-one 5 5-methyl-2-(5-((4-methylpiperazin-l-yl)sulfonyl)-2-propoxyphenyl)-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde 2-(5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7-propyl- 10 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde 2-(5-((4-(2-hydroxyethyl)piperazm-l-yl)sulfonyl)-2-propoxyphenyl)-6-(hydroxymethyl)-5- methyl-7-propylpyrrolo[2, 1-f][1,2,4]triazin-4(3H)-one CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 (7r)-5-methyl-2-(5-((4-methylpiperazin-l-yl)sulfonyl)-2-propoxyphenyl)-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][l ,2,4]triazine-6-carbaldehyde O-methyl oxime 5 2-(5-((4-(2-hydroxyethyl)piperidin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde 2-(l-((3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f] [l,2,4]triazin-2-yl)-4- propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate (E)-2-(5-((4-(2-hydroxycthyl)piperidin-l-yl)sulfonyl)-2-propoxyphcnyl)-5-methyl-4-oxo-7- propy1-3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde oxime CA 03001728 2018-04-11 WO 2017/085056 — 27 — PCT/EP2016/077720 ethyl-3-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-6-yl)-3-hydroxypropanoate 5 3-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-metliyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-6-yl)-N,3-dihydroxypropanamide 3-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-6-yl)-3-hydroxypropanenitrile (/r)-2-(l-((3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazm-2-yl)-4-propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde ° (lw); 5 (£')-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-1-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde oxime 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldchyde O-methyloximc (jE)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazme-6-carbaldehyde O-(2-hydroxyethyl) oxime OH (1Z); CA 03001728 2018-04-11 WO 2017/085056 -29- PCT/EP2016/077720 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazm-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde (E)-2-(2-cthoxy-5-((4-(2-hydroxycthyl)pipcrazin-l-yl)sulfonyl)phcnyl)-5-cthyl-4-oxo-7-propyl- 5 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde oxime (E)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazm-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde O-methyl oxime 10 (E)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l -yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde O-(2-hydroxyethyl) oxime 2-(4-((4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazin-2- yl)phenyl)sulfonyl)piperazin- 1-yl)ethyl nitrate 15 O (lae); CA 03001728 2018-04-11 WO 2017/085056 -30- PCT/EP2016/077720 (E)-2-(4-((4-cthoxy-3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin- -yl)ethyl1 nitrate 2-(4-((4-ethoxy-3-(5-ethyl-6-formyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazin-2- 5 yl)phenyl)sulfonyl)piperazin- 1-yl)ethyl nitrate O II ’O O' (lag); (E)-2-(4-((4-ethoxy-3-(5-ethyl-6-((hydroxyimino)methyl)-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin- -yl)ethyl1 nitrate O II .Nt O O' (lah) 10 (l£)-2-(4-((4-ethoxy-3-(6-((methoxyimmo)methyl)-5-rnethyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin- -yl)ethyl1 nitrate o II O O' o (lai) (E)-2-(4-((4-ethoxy-3-(6-(((2-hydroxyethoxy)imino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin- -yl)ethyl1 nitrate CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 (lak) (E)-2-(2-cthoxy-5-((4-(2-hydroxycthyl)pipcridin-l-yl)sulfdnyl)phcnyl)-5-rncthyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2, 1-f][1,2,4]triazine-6-carbaldehyde oxime 0 (lai) 5 (£)-2-(1-((4-ethoxy-3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperidin-4-yl)ethyl nitrate O (lam) (£)-2-(2-cthoxy-5-((4-(3-hydroxypropyl)pipcrazin-l-yl)sulfonyl)phcnyl)-5-mcthyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde oxime (E)-3-(4-((4-ethoxy-3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin- -yl)propyl1 nitrate CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 O' (lao) 2-(2-ethoxy-5-((4-(3-hydroxypropyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-7-propylpyrrolo 5 (Z)-2-(l-((3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazin-2-yl)-4-propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate In another very preferred embodiment, said compound of formula I is selected from 10 (E)-2-(5-((4-(2-hydroxycthyl)pipcridin-1-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde oxime (lr);and (E)-2-(l-((3-(6-((hydroxyimino)mcthyl)-5-mcthyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazin-2-yl)-4-propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate (Iv). 15 In another very preferred embodiment, said compound of formula I is (JE')-2-(5-((4-(2- hydroxyethyl)piperidin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f|[l,2,4]triazine-6-carbaldehyde oxime (lr). CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -33 - In another very preferred embodiment, said compound of formula I is (£')-2-(l-((3-(6- ((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)- 4-propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate (Iv). 5 In another very preferred embodiment, said compound of formula I is (E')-2-(2-ethoxy-5-((4- methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][l,2,4]triazine-6-carbaldehyde oxime (la). In another very preferred embodiment, said compound of formula I is (2T)-5-methyl-2-(5-((4- 10 methylpiperazin-l-yl)sulfonyl)-2-propoxyphenyl)-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f|[l,2,4]triazine-6-carbaldchydc O-methyl oxime (lo). In another very preferred embodiment, said compound of formula I is (E)-2-(5-((4-(2- hydroxyethyl)piperidin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7-propyl-3,4- 15 dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde oxime (Ir). In another very preferred embodiment, said compound of formula I is ethyl-3-(2-(2-ethoxy-5- ((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazin-6-yl)-3-hydroxypropanoate (Is). 20 In another very preferred embodiment, said compound of formula I is 3-(2-(2-ethoxy-5-((4- methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazin-6-yl)-N,3-dihydroxypropanamide (It). 25 In another very preferred embodiment, said compound of formula I is 3-(2-(2-ethoxy-5-((4- methylpiperazin- 1-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,1- f][1,2,4]triazin-6-yl)-3-hydroxypropanenitrile (lu). In another very preferred embodiment, said compound of formula I is 2-(2-ethoxy-5-((4-(2- 30 hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazine-6-carbaldehyde_(lw). CA 03001728 2018-04-11 _ _ WO 2017/085056 34 PCT/EP2016/077720 In another very preferred embodiment, said compound of formula I is (E')-2-(2-cthoxy-5-((4-(2- hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][ 1,2,4]triazine-6-carbaldehyde O-(2-hydroxyethyl) oxime (Iz). 5 In another very preferred embodiment, said compound of formula I is (E')-2-(2-ethoxy-5-((4-(2- hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][l,2,4]triazine-6-carbaldehyde oxime (lab). In another very preferred embodiment, said compound of formula I is (£)-2-(2-ethoxy-5-((4-(2- 10 hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f|[l,2,4]triazine-6-carbaldchydc O-(2-hydroxyethyl) oxime (lad). In another very preferred embodiment, said compound of formula I is (K)-2-(2-ethoxy-5-((4-(2- hydroxyethyl)piperazin- 1-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2, 1- 15 f|[l,2,4]triazine-6-carbaldehyde O-(2-hydroxyethyl) oxime (lad). In another very preferred embodiment, said compound of formula I is 2-(4-((4-ethoxy-3-(6- formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2, 1-f][1,2,4]triazin-2- yl)phenyl)sulfonyl)piperazin-l-yl)ethyl nitrate (lae). 20 In another very preferred embodiment, said compound of formula I is (F)-2-(4-((4-ethoxy-3-(6- , ((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2 1-f][1,2,4]triazin-2- yl)phenyl)sulfonyl)piperazin- 1-yl)ethyl nitrate (lai). 25 In another very preferred embodiment, said compound of formula I is (£)-2-(4-((4-cthoxy-3-(5- ethyl-6-((hydroxyimino)methyl)-4-oxo-7-propyl-3,4-dihydropyrrolo[2, 1-f][1,2,4]triazin-2- yl)phenyl)sulfonyl)piperazin-l-yl)ethyl nitrate (lah). In another very preferred embodiment, said compound of formula I is (E)-2-(4-((4-cthoxy-3-(6- 30 (((2-hydroxyethoxy)imino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin-l-yl)ethyl nitrate (lak). CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -35 - In another very preferred embodiment, said compound of formula I is (E')-2-(2-cthoxy-5-((4-(2- hydroxyethyl)piperidin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][l,2,4]triazine-6-carbaldehyde oxime (lai). 5 In another very preferred embodiment, said compound of formula I is (E)-2-(l-((4-ethoxy-3-(6- , ((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2 1-f][1,2,4]triazin-2- yl)phenyl)sulfonyl)piperidin-4-yl)ethyl nitrate (lam). In another very preferred embodiment, said compound of formula I is (£)-2-(2-ethoxy-5-((4-(3- 10 hydroxypropyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde oxime (Ian). In another very preferred embodiment, said compound of formula I is (£')-3-(4-((4-cthoxy-3-(6- ((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazin-2- 15 yl)phenyl)sulfonyl)piperazin-l-yl)propyl nitrate (lao). It has been shown that compounds of the present invention are potent and selective inhibitors of cGMP specific PDE. Furthermore, it has been found that the compounds of the present invention can be tailored to become dual-pharmacology NO-releasing PDE5 inhibitors which are believed 20 to release NO in addition to its PDE 5 inhibition in a more than additive fashion. Thus, compounds of formula I are of interest for use in therapy, specifically for the treatment of a variety of conditions where inhibition of cGMP specific PDE is thought to be beneficial. Thus, in a further aspect, the present invention provides for a pharmaceutical composition 25 comprising at least one of the inventive compound of formula I, or a pharmaceutically acceptable salt, solvate or hydrate thereof, and a pharmaceutically acceptable excipient, adjuvant, or carrier. In another aspect, the present invention provides for a pharmaceutical composition comprising exactly one inventive compound of formula I, or a pharmaceutically acceptable salt, solvate or hydrate thereof, and a pharmaceutically acceptable excipient, 30 adjuvant, or carrier. Pharmaceutically acceptable excipient, adjuvant, or carrier are known to the skilled person in the art. In another aspect, the present invention provides for a compound of formula I, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for use as a pharmaceutical. In again CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -36 - another aspect, the present invention provides for a compound of formula I, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for use as an animal medicament. It has surprisingly been found that the compounds of the present invention are very potent and 5 selective inhibitors of PDE5. Furthermore, we have surprisingly found that the compounds of the present invention can be tailored to become dual-pharmacology NO-releasing PDE5 inhibitors which are believed to release NO in addition to its PDE 5 inhibition in a more than additive fashion. Moreover, it has surprisingly been found that preferred compounds of the present invention show even a significantly higher PDE5 inhibiton activity as compared to known PDE5 10 inhibitors such as sildenafil. As a consequence, the novel pyrrolo triazine compounds of the present invention are useful in the therapy and prophylaxis of diseases which are associated with a disturbed cGMP balance. In particular, the compounds of the present invention are potent and selective inhibitors of cyclic guanosine 3’-5’-monophosphate specific phosphodiesterase 5 (cGMP specific PDE5) and thus have utility in variety of therapeutic areas where such inhibition 15 is thought to be beneficial. Some of the preferred therapeutic areas are wound healing, in particular chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Raynaud’s, male erectile dysfunction, female sexual dysfunction, Alzheimer’s disease, diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension and chronic heart failure. 20 As a consequence of the selective PDE5 inhibition exhibited by compounds of the present invention, cGMP levels are expected to be elevated, which in turn can give rise to beneficial anti-platelet, anti-vasospastic, vasodilatory, natriuretic and diuretic activities as well as potentiation of the effects of endothelium-derived relaxing factor (EDRF) nitric oxide (NO), nitrovasodilators, atrial natriuretic factor (ANF), brain natriuretic peptide (BNP), C-type 25 natriuretic peptide (CNP) and endothelium-dependent relaxing agents such as bradykinin, acetylcholine and 5-HTi. The compounds of formula I therefore have utility in the treatment of a number of disorders, including stable, unstable and variant (Prinzmetal) angina, hypertension, pulmonary hypertension, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency (e.g. post-percutaneous transluminal coronary angioplasty), 30 peripheral vascular disease, vascular disorders such as Raynaud's disease, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, diabetes, glaucoma and diseases characterized by disorders of gut motility like irritable bowel syndrome, wound healing, in particular chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Alzheimer’s CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -37 - disease, hair loss, skin aging, vascular aging, pulmonary artery hypertension and chronic heart failure. Thus, in another aspect, the present invention provides for a compound of formula I, or a 5 pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for use in a method of treating or preventing a disease alleviated by inhibition of PDE-5 in a human or in a non-human mammal, preferably in a human. Preferably, said disease is selected from wound healing, chronic wound healing, diabetic foot ulcer, leg ulcer, Raynaud’s disease, male erectile dysfunction, female sexual dysfunction, hair loss, skin aging, vascular aging, 10 pulmonary artery hypertension; stable, unstable and variant (Prinzmetal) angina; hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, systemic sclerosis (SSc), scleroderma, morphea, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, diabetic neuropathy, 15 Idiopathic pulmonary fibrosis (IPF), peyronic’s disease, diabetes, glaucoma or a disease characterized by disorders of gut motility like irritable bowel syndrome, liver fibrosis, Alzheimer’s disease and chronic heart failure, wherein further preferably said disease is selected from wound healing, chronic wound healing, diabetic foot ulcer, leg ulcer, diabetic neuropathy, peripheral vascular disease, vascular disorders, Raynaud's disease, systemic sclerosis (SSc), 20 scleroderma, pulmonary artery hypertension, diabetes, male erectile dysfunction, and wherein again further preferably said disease is selected from wound healing, chronic wound healing, diabetic foot ulcer, leg ulcer and diabetic neuropathy. In again another aspect, the present invention provides for a compound of formula I, or a 25 pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for use in a method of treating or preventing a disease by inhibition of PDE-5 in a human or in a non-human mammal, preferably in a human. In again another aspect, the present invention provides for a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for use in a method of treating a medical condition in 30 a human or in a non-human mammal, preferably in a human, wherein for said medical condition inhibition of PDE5 is desired. In again another aspect, the present invention provides use of a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -38 - for the manufacture of a medicament for the treatment or prevention of a disease by inhibition of PDE-5 in a human or in a non-human mammal, preferably in a human. In again another aspect, the present invention provides use of a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for the manufacture of a 5 medicament for the treatment or prevention of a disease alleviated by inhibition of PDE-5 in a human or in a non-human mammal, preferably in a human. In again another aspect, the present invention provides use of a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof, for the manufacture of a medicament for the treatment a medical condition in a human or in a non-human mammal, 10 preferably in a human, wherein for said medical condition inhibition of PDE5 is desired. In again another aspect, the present invention provides for a method of treating or preventing a disease by inhibition of PDE-5 in a human or in a non-human mammal, preferably in a human, comprising administering to said human or said non-human mammal, preferably to said human 15 an effective amount of a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof. In again another aspect, the present invention provides for a method of treating or preventing a disease alleviated by inhibition of PDE-5 in a human or in a non-human mammal, preferably in a human, comprising administering to said human or said non-human mammal, preferably to said human an effective amount of a 20 compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof. In again another aspect, the present invention provides for a method of treating a medical condition in a human or in a non-human mammal, preferably in a human, wherein for said medical condition inhibition of PDE5 is desired, comprising administering to said human or said non-human mammal, preferably to said human an effective amount of a 25 compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable salt, solvate or hydrate thereof. In a preferred embodiment of the present invention, said disease or said a medical condition is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot 30 ulcer, leg ulcer, Raynaud’s disease, male erectile dysfunction, female sexual dysfunction, diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension; stable, unstable, and variant (Prinzmetal) angina; hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, systemic sclerosis (SSc), CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -39 - scleroderma, morphea, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, diabetic neuropathy, Idiopathic pulmonary fibrosis (IPF), peyronic’s disease, glaucoma or a disease characterized by disorders of gut motility like irritable bowel syndrome, liver fibrosis, Alzheimer’s disease and chronic heart failure, wherein preferably said 5 disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, diabetic neuropathy, peripheral vascular disease, vascular disorders such as Raynaud's disease, systemic sclerosis (SSc), scleroderma, pulmonary artery hypertension, diabetes, male erectile dysfunction, and wherein again further preferably said disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg 10 ulcer and diabetic neuropathy. There is thus provided as a further aspect of the present invention a compound of formula I for use in the treatment of wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Raynaud’s disease, male erectile dysfunction, female sexual dysfunction, 15 diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension; stable, unstable, and variant (Prinzmetal) angina; hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, systemic sclerosis (SSc), scleroderma, morphea, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic 20 asthma, allergic rhinitis, diabetic neuropathy, Idiopathic pulmonary fibrosis (IPF), peyronic’s disease, glaucoma or a disease characterized by disorders of gut motility like irritable bowel syndrome, liver fibrosis, Alzheimer’s disease and chronic heart failure, wherein preferably said disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, diabetic neuropathy, peripheral vascular disease, vascular disorders such as 25 Raynaud's disease, systemic sclerosis (SSc), scleroderma, pulmonary artery hypertension, diabetes, male erectile dysfunction, and wherein again further preferably said disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer and diabetic neuropathy. 30 According to another aspect of the invention, there is provided the use of a compound of formula I for the manufacture of a medicament for the treatment of wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Raynaud’s disease, male erectile dysfunction, female sexual dysfunction, diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension; stable, unstable, and variant (Prinzmetal) angina; hypertension, CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, vascular disorders, systemic sclerosis (SSc), scleroderma, morphea, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, diabetic neuropathy, 5 Idiopathic pulmonary fibrosis (IPF), peyronic’s disease, glaucoma or a disease characterized by disorders of gut motility like irritable bowel syndrome, liver fibrosis, Alzheimer’s disease and chronic heart failure, wherein preferably said disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, diabetic neuropathy, peripheral vascular disease, vascular disorders such as Raynaud's disease, systemic sclerosis 10 (SSc), scleroderma, pulmonary artery hypertension, diabetes, male erectile dysfunction, and wherein again further preferably said disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer and diabetic neuropathy. In a further aspect, the invention provides a method of treating wound healing, preferably 15 chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, Raynaud’s disease, male erectile dysfunction, female sexual dysfunction, diabetes, hair loss, skin aging, vascular aging, pulmonary artery hypertension; stable, unstable, and variant (Prinzmetal) angina; hypertension, pulmonary hypertension, chronic obstructive pulmonary disease, congestive heart failure, renal failure, atherosclerosis, conditions of reduced blood vessel patency, peripheral vascular disease, 20 vascular disorders, systemic sclerosis (SSc), scleroderma, morphea, inflammatory diseases, stroke, bronchitis, chronic asthma, allergic asthma, allergic rhinitis, diabetic neuropathy, Idiopathic pulmonary fibrosis (IPF), peyronic’s disease, glaucoma or a disease characterized by disorders of gut motility like irritable bowel syndrome, liver fibrosis, Alzheimer’s disease and chronic heart failure, wherein preferably said disease is selected from wound healing, preferably 25 chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer, diabetic neuropathy, peripheral vascular disease, vascular disorders such as Raynaud's disease, systemic sclerosis (SSc), scleroderma, pulmonary artery hypertension, diabetes, male erectile dysfunction, and wherein again further preferably said disease is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer, leg ulcer and diabetic neuropathy in a human or 30 in non-human mammal, preferably in a human, said method comprises administering to said human or said non-human mammal, preferably to said human, an effective amount of a compound of formula I. CA 03001728 2018-04-11 _ _ WO 2017/085056 41 PCT/EP2016/077720 In a very preferred embodiment of the present invention, said disease or said a medical condition is selected from wound healing, preferably chronic wound healing, diabetic foot, diabetic foot ulcer and leg ulcer. 5 Chronic, non-healing skin wounds such as in diabetes mellitus are governed by complex disease mechanisms including impaired angiogenesis, defective microcirculation, and endothelial dysfunction. Diabetic foot ulcer and chronic wounds are a major source of morbidity and is a leading cause of hospitalizations in diabetic patients. It afflicts 15% of diabetes patients (275 Mio) and is a huge burden to patients and payers (12 billion $ / year). 3-4% of all diabetic 10 patients will get lower limb amputations every year. Ultra-potent PDE5 inhibitors or compounds integrating highly potent inhibition of PDE5 and activation of nitric oxide dependent soluble guanylate cyclase as the ones of the present invention can be expected to accelerate wound healing. 15 As used herein, the terms “treatment”, “treat”, “treated” or “treating” refer to prophylaxis and/or therapy. In one embodiment, the terms “treatment”, “treat”, “treated” or “treating” refer to a therapeutic treatment. In another embodiment, the terms “treatment”, “treat”, “treated” or “treating” refer to a prophylactic treatment. Preferably, beneficial or desired clinical results of said treatment include, but are not limited to, alleviation of symptoms, diminishment of extent of 20 disease or medical condition, stabilized (i.e., not worsening) state of disease or medical condition, delay or slowing of disease or medical condition progression, amelioration or palliation of the disease or medical condition state. As used herein, the term “effective amount” refers to an amount necessary or sufficient to realize 25 a desired biologic effect. Preferably, the term “effective amount” refers to an amount of a compound of formula I of the present invention that (i) treats or prevents the particular disease, medical condition, or disorder, (ii) attenuates, ameliorates, or eliminates one or more symptoms of the particular disease, medical condition, or disorder, or (iii) prevents or delays the onset of one or more symptoms of the particular disease, medical condition, or disorder described herein. 30 An effective amount of the inventive compound of formula I, or said pharmaceutical composition, would be the amount that achieves this selected result, and such an amount could be determined as a matter of routine by a person skilled in the art. Further preferably, the term “effective amount”, as used herein, refers to an amount necessary or sufficient to be effective to increase the inhibition of PDE5, typically and preferably as determined in Example 64, or to CA 03001728 2018-04-11 WO 2017/085056 — 42 — PCT/EP2016/077720 increase the formation of cGMP, typically and preferably as determined in Example 65. The effective amount can vary depending on the particular composition being administered and the size of the subject. One of ordinary skill in the art can empirically determine the effective amount of a particular composition of the present invention without necessitating undue 5 experimentation. The term "mammal", as used herein, includes, but is not limited to, humans, mice, rats, guinea pigs, monkeys, dogs, cats, horses, cows, pigs, and sheep. The term "mammal", as used herein, preferably refers to humans. 10 The compounds of formula I and the pharmaceutical compositions of the present invention may be administered by any suitable route, for example by oral, buccal, sub-lingual, rectal, vaginal, nasal, topical or parenteral administration, which forms another aspects of the present invention. 15 In again another aspect, the present invention provides for a compound of the formula II: wherein Rj, R2, R3 are defined as for the compound of formula I. In still a further aspect, the present invention provides for a compound of formula IV 20 wherein X, Ri, R2, R3, R4, Rs are defined as for the compound of formula I. In again another aspect, the present invention provides for a process for the preparation of a compound of formula I, CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -43 - wherein said process comprises: (a) reaction of a compound of formula II with a benzoic acid derivative of formula III in an aprotic or a protic solvent to generate a compound of formula IV III (b) cyclization of said compound of formula IV to yield compound of formula I, wherein X, Ri, R2, R3, R4, and R5 are defined as for the compound of formula I. 10 In again another aspect, the present invention provides for a process for the preparation of a compound of formula I, wherein said process comprises (a) reaction of a compound of formula VI with a benzoyl chloride derivative of formula VIA to generate a compound of formula VII CA 03001728 2018-04-11 WO 2017/085056 — 44— PCT/EP2016/077720 VI VIA VII (b) hydrolysis of the ester compound of formula VII to an acid derivative of formula VIII (c) amination of said compound of formula VIII to yield a compound of formula IV (d) cyclization of said compound of formula IV to yield compound of formula I, wherein X, Ri, R2, R3, R4, and R5 are defined as for the compound of formula I; and wherein R’ is C1-C4 alkyl, benzyl, 4-alkoxybenzyl. 10 In again another aspect, the present invention provides for a process for the preparation of a compound of formula I, wherein said process comprises conversion of compound of formula IA to yield compound of formula I Rs IA wherein X, Ri, R2, R3, R4, and R5 are defined as for the compound of formula I in any one of the 15 claims 1 to 8. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -45 - Compounds of formula I may be prepared by the following reaction SCHEME 1 and reaction SCHEME 2. These schemes represent the synthesis of generic compounds of formula I and forms part of the present invention. 5 SCHEME 1: Thus, a process for preparing compounds of formula I involves synthesis of intermediate II 10 which is a precursor to compound of formula IV which acts as a precursor to other compounds of formula I. Compound of formula IV is synthesized alternatively as shown in SCHEME 2, starting from compound of formula V, which is subsequently converted to compound of formula VI, followed by compound VII and VIII; SCHEME 2 thus forms an alternate route for the synthesis of the inventive compound of formula I. 15 Compound of formula II is reacted with a benzoic acid derivative of formula III in an aprotic or a protic solvent, selected from the group comprising DMF, acetonitrile, dialkylether, chlorinated hydrocarbons. The reaction is performed in the presence of a condensation reagent like e.g. thionyl chloride, phosphoroxy chloride, carbodiimide reagents like DCC, EDC or DCI, HBTU in 20 presence or absence of bases to generate a compound of formula IV, which undergoes a reductive cyclization in the presence of bases like potassiumhydroxide, potassium tert-butoxide CA 03001728 2018-04-11 WO 2017/085056 -46- PCT/EP2016/077720 in solvents like butanol, poly-ethyleneglycol, DMF to generate compound of formula IA. Compound IA under various reaction conditions is converted to compounds of the type I. SCHEME 2: 5 IA I Wherein R’ typically and preferably comprises: C1-C4 alkyl, benzyl, 4-alkoxybenzyl. The above alternate reaction sequence can be schematically represented as follows, wherein the substitutions R’ is as defined above and Ri is typically and preferably methyl, R2 is typically and 10 preferably formyl; R3 is typically and preferably propyl, R4 is typically and preferably propoxy, Rs is typically and preferably hydrogen. For the conversion of compound of formula V to VI, any amination reagent can be employed like NH4OH / NaOCl or the like. The reaction is performed in the presence of a base optionally CA 03001728 2018-04-11 _ _ WO 2017/085056 47 PCT/EP2016/077720 under phase transfer conditions using phase transfer catalyst. The base that can be employed is NaOH, KOH, potassium tert-butoxide or the like. The phase transfer catalyst that can be employed for this purpose are benzyltrimethylammonium chloride, benzyltriethylammonium chloride, methyltricaprylammonium chloride, methyltributylammonium chloride, and 5 methyltrioctylammonium chloride or the like. The reaction is performed in the presence of water and a another solvent which is typically and preferably selected from chlorinated hydrocarbon solvents like dichloromethane, 1,2 dichloroethane, ether solvents like diethyl ether, tertiary butyl ether or the like; The reaction is preferably performed at temperatures ranging from between -20 to 30° C. 10 The thus obtained aminated product of the formula VI is reacted with a suitable activated benzoic acid compounds, preferably benzoic acid chloride. The reaction is performed in the presence of suitable amines, tertiary amine like diisopropylethylamine being the most preferable. The reaction is carried out in the presence of aprotic cyclic hydrocarbon solvent or halo- 15 hydrocarbons wherein toluene and methylene chloride being the most preferable. The reaction is typically and preferably performed in the temperatures ranging from -20 to 30°C. The ester moiety of the compound of VII is hydrolyzed in the presence of alkali hydroxides like NaOH, LiOH, KOH and the reaction can be performed in water, alcohols like ethanol, propanol n- butanol or the like, cyclic ethers like tetrahydrofuran. 20 Amination of compound of formula VIII can be carried out by any amination reagents, wherein any ammonium salt can be employed, NH4CI or NH4OAC being the most preferable. The reaction is performed in the presence of a condensation reagent like thionyl chloride, phosphoroxy chloride, carbodiimide reagents like DCC, EDC or DCI, wherein HBTU being the 25 most preferred. The solvents that can be employed are aprotic solvents like amides, ethers and hydrocarbons like dimethylformamide, tetrahydrofuran, methylenechloride. The reaction is typically and preferably performed at temperatures ranging from -20 to 30°C. The thus obtained compound IV is converted to I by cyclization to the triazine. The cyclization can be carried out in the presence of strong bases like potassium tert-butoxide. The reaction is preferably carried 30 out in the presence of solvents like alcohols, wherein the preferred alcoholic solvent is tertiary butanol or PEG 400 or similar polyether solvents. The temperature of the reaction can range between 120 - 160 °C. CA 03001728 2018-04-11 WO 2017/085056 -48- PCT/EP2016/077720 Intermediate II of SCHEME 1, wherein R2 is formyl is a preferred embodiment of the present invention. Thus, for example, a specific process for preparing one of the compound falling under the group 5 of compounds II, of formula 2a comprises treating ethyl-3-oxobutanoate with acetic acid/sodium nitrite to generate an oxime (Z)-ethyl-2-(hydroxyimino)-3-oxobutanoate (5a) by nitrosation of the active methylene group. Compound 5a on condensation with (E)-hex-2-enal followed by cyclisation yield a pyrrole 10 derivative, ethyl-4-formyl-l-hydroxy-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (6a), which on Zinc/acetic acid reduction yield ethyl-4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (7a) Carboxylate compound 7a was derivatized to the corresponding amide 8a which is subsequently 15 converted to the intermediate 2a, l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2- carboxamide. SCHEME 3: Zn, AcOH DCM, 30min. RT 7a NH4C1, hbtu DIPEA, DMT 16h RT CA 03001728 2018-04-11 WO 2017/085056 -49- PCT/EP2016/077720 Compounds of formula II are preferred intermediates for the synthesis of compounds of formula I. Thus in accordance with the present invention, intermediate II, preferably when R2 = formyl, is 5 subsequently reacted with benzoic acid derivative of the formula III Rs III wherein R4 and R5 are as defined above to generate Rs IVA which is cyclized to a pyrrolo triazine compound 10 R* IA Compound IA is subsequently converted to various compounds of formula I, like la, lb, 1c, Id, le, If, 1g by oxidation, reduction, condensation or the like. 15 As an example, compound IA, when Ri is methyl, R2 is CHO, Rjis propyl, R4 is ethoxy, R5 is SO2NR10R11, wherein R10R11 together form HN represents CA 03001728 2018-04-11 WO 2017/085056 -50- PCT/EP2016/077720 Compound Ij is a novel compound and forms yet another part of the invention Starting from compound 1j, under various reaction conditions, compounds la, lb, 1c, Id, le, If, 5 1g are prepared, as in SCHEME 3 and forms another part of the present invention. Thus, compound Ij is oxidized in presence of suitable oxidizing agents like sodium chlorite, in the presence of solvents like acetonitrile, tetrahydrofurane or tert-butanol to generate compound of formula Id. Compound Id is converted to corresponding methyl ester If by treatment with 10 methanol and thionyl chloride; Id on the other hand is converted to an amide derivative le which is subsequently converted to a hydroxamic acid 1g. Compound Ij is converted to an aldoxime of the type la which in turn generated a hydroxymethyl derivative lb. Compound Ij is subsequently converted into a methylester of the 15 type 1c. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -51 - Likewise, compounds Ih and li are synthesized starting from Ij. 5 EXAMPLES Synthesis of some of the compounds of formula I are exemplified below. The following 10 examples further illustrate the present invention, but should not be construed in any way as to limit its scope. EXAMPLE 1 (Z)-ethyl 2-(hydroxyimino)-3-oxobutanoate (5a) To a stirring solution of ethyl 3-oxobutanoate (500 mg, 3.84 mmol) in acetic acid (5 mL) was 15 added sodium nitrate (330 mg, 4.50 mmol) in water (10 mL) and the resultant reaction mixture was stirred at RT for 16 h. The reaction mixture was quenched with saturated ammonium chloride solution and extracted with dichloromethane (2 x 20 mL). The combined CA 03001728 2018-04-11 WO 2017/085056 -52- PCT/EP2016/077720 dichloromethane layer was washed with brine and dried over anhydrous sodium sulfate and 1 concentrated under reduced pressure to afford the title compound as a solid (750 mg). H NMR (300MHz, CDC13) 8 = 9.09 (br s, 1H), 4.39 (q, 7=7.2 Hz, 2H), 2.41 (s, 3H), 1.36 (t, 7=7.3 Hz, 3H), Mass (M-H) =158.1. 5 EXAMPLE 2 Ethyl 4-formyl-l-hydroxy-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (6a) (Z)-ethyl 2-(hydroxyimino)-3-oxobutanoate (5a) (10 g, 62.83 mmol), (E)-hex-2-enal (12.25 g, 125.7 mmol) in toluene, was added di-isopropylamine (1.26 g, 12.56 mmol) and the resultant reaction mixture was stirred at RT for 16 h. The reaction mixture was quenched with aqueous 10 Ammonium chloride solution (100 mL) and extracted with dichloromethane (2 x200 mL). The combined dichloromethane layer was washed with brine and dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification by column chromatography (100- 200 mesh silica gel, 0-5% ethyl acetate in pet ether as eluent) afforded the title compound as a solid (10.5 g). ’H NMR (400MHz, CDC13) 8 = 12.35 (d, 7=1.4 Hz; 1H), 9.95 (d, 7=1.4 Hz; 1H), 15 4.51 - 4.35 (m, 2H), 3.02 - 2.85 (m, 2H), 2.56 (d, 7=1.4 Hz; 3H), 1.77 - 1.62 (m, 2H), 1.43 (dt, 7=1.2, 7.1 Hz; 3H), 1.04 - 0.91 (m, 3H), Mass (M+H) =240.1. EXAMPLE 3 Ethyl 4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (7a) To a stirring solution of ethyl 4-formyl-l-hydroxy-3-methyl-5-propyl-lH-pyrrole-2-carboxylate 20 (6a) (18 g, 75.51 mmol) in dichloromethane (1.8 L), were added zinc dust (34 g, 527.1 mmol) and acetic acid (74 mL) and the resultant reaction mixture was stirred at RT for 30 min. The reaction mixture was quenched with saturated sodium bicarbonate solution and adjusted PH to 7 then extracted with dichloromethane (2 x250 mL). The combined dichloromethane layer was washed with brine and dried over anhydrous sodium sulfate and concentrated under reduced 25 pressure. Purification by column chromatography (100-200 mesh silica gel, 5% methanol in dichloromethane as eluent) afforded the title compound as a solid (8 g). [SM was recovered by eluting with 5% ethyl acetate in pet ether as eluent], 'H NMR (300MHz, CDCI3) 8 = 10.01 (s, 1H), 9.04 (br s, 1H), 4.34 (q, 7=7.4 Hz, 2H), 2.95 - 2.84 (m, 2H), 2.58 (s, 3H), 1.70 (qd, 7=7.3, 14.9 Hz, 2H), 1.38 (t, 7=7.0 Hz, 3H), 0.98 (t, 7=7.3 Hz, 3H), LCMS (M+H) =224.1, purity=68%. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -53 - EXAMPLE 4 4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxamide (8a) and 4-formyl-3-methyl-5- propyl-lH-pyrrole-2-carboxylic acid (8b) To a stirring solution of ethyl 4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (7a) (13 g, 5 58.2 mmol) in ethanol (30 mL), was added ammonium hydroxide (130 mL) and the resultant reaction mixture was heated to 100 °C for 16 h. The reaction mixture was concentrated under reduced pressure and the obtained crude compound was used in the next step reaction without further purification. MS indicates the presence of mixture of products (mixture of 8a and 8b). EXAMPLE 5 10 4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxainide (8a) The above mixture (8a + 8b) in dimethylformamide (40 mL), was treated with HBTU (27.9 g, 73.84 mmol) and diisoproylamine (1.58 g, 123.08 mmol) and stirred for RT under nitrogen atmosphere. To this ammonium chloride (4.88 g, 92.22 mmol) was added and the resultant reaction mixture was stirred at RT for 16 h. The reaction mixture was diluted with ethyl acetate 15 (300 mL) and washed with brine and dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification was done by ether washings (3x 20 mL) to afford the title compound as a solid (9.2 g). ’H NMR (400MHz, DMSO-d6) 8 = 11.61 (br s, 1H), 9.89 (s, 1H), 7.10 (br s, 2H), 2.80 (br t, J=7.4 Hz, 2H), 2.45 (s, 3H), 1.68 - 1.52 (m, 2H), 0.87 (br t, J=1.2 Hz, 3H), Mass (M-H) =193.3. 20 EXAMPLE 6 l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxamide (2a) To a stirring solution of 4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxamide (8a) (3.0 g, 15.46 mmol) in NMP, was added potassium tert-butoxide (17 mL, 17.0 mmol, IM) and stirred for 20 °C for 2 h. To this O-(4-nitrobenzoyl)-hydroxylamine (3.37 g, 18.55 mmol) in NMP was 25 added slowly and the resultant reaction mixture was stirred at RT for 30 min. [Purple color reaction mixture indicates the formation of product].The reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (3 x70 mL). The combined ethyl acetate layer was washed with brine and dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification was done by ether washings (3x30 mL) to afford the title compound as a 30 solid. JH NMR (400MHz, DMSO-d6) 8 = 11.61 (br s, 1H), 9.85 (s, 1H), 7.87 (br s, 1H), 7.43 (br CA 03001728 2018-04-11 WO 2017/085056 -54- PCT/EP2016/077720 s, 2H), 2.93 - 2.83 (m, 2H), 2.41 (s, 3H), 1.60 - 1.52 (m, 2H), 0.91 (br t, J=12 Hz, 3H), Mass (M+H) =210.3. 5 SCHEME 5: EXAMPLE 7 10 5-(chlorosulfonyl)-2-ethoxybenzoic acid (9) 2-ethoxybenzoic acid (25 g) at 25°C was added to a mixture of thionyl chloride (11 mL), and chlorosulfonic acid (41.3 mL) and the resultant reaction mixture was stirred at RT for 16 h. An off white solid was separated out which is stirred for 1 h. And the reaction mixture was quenched CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -55 - with ice (270 g) and water (60 mL). The obtained solid was separated by filtration and solid was washed with water (2x100mL) and dried under vacuum to afford the title compound as a solid (30 g). 'H NMR (400MHz, CDC13) 6 = 8.83 (s, 1H), 8.20 (br d, J=8.8 Hz, 1H), 7.23 (d, J=8.8 Hz, 1H), 4.45 (q, ./=6.6 Hz, 2H), 1.64 (t, ./=6.6 Hz, 3H), LCMS (M-H) =263.1, purity=95%. 5 EXAMPLE 8 2-ethoxy-5-(4-methylpiperazin-l-ylsulfonyl)benzoic acid (10) To a stirring solution of 5-(chlorosulfonyl)-2-ethoxybenzoic acid (9) (30 g) in water (124 mL) at 10 °C, was added N-methyl piperazine (33.6 mL) at 15-20 °C. The resultant reaction mixture was stirred at 10 °C. After 5 min the title compound started to crystallize and the reaction 10 mixture was stirred for 2 h. The solid was separated by filtration; the solid was washed with water and dried under vacuum. Purification was done by heating in acetone (100 mL) for 1 h. The suspension was cooled to RT, crystallized solid was separated by filtration and dried under 1 vacuum to afford the title compound as a white solid (23 g). H NMR (400MHz, DMSO-d6) 8 = 7.89 (br d, J=1.9 Hz, 1H), 7.81 (br dd, J=1.9, 8.8 Hz, 1H), 7.35 (br d, J=8.8 Hz, 1H), 4.21 (q, 15 ./=6.8 Hz, 2H), 2.87 (br s, 4H), 2.37 (br s, 4H), 2.14 (s, 3H), 1.36 (t, ,/=7.0 Hz, 3H), LCMS (M+H) =329.1, purity=88%. EXAMPLE 9 l-amino-N-(2-ethoxy-5-(4-methylpiperazin-l-ylsulfonyl)benzoyl)-4-formyl -3-methyl-5- propyl-lH-pyrrole-2-carboxamide (11) 20 To a stirring solution of 2-ethoxy-5-(4-methylpiperazin-l-ylsulfonyl)benzoic acid (10) (3.01g, 9.186 mmol) in DMF, was added HBTU (5.79 g, 15.3 mmol) and diisopropylethyl amine (2.46 g, 19.12 mmol) to this l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxamide (2a) (1.6 g, 7.65 mmol) was added and the resultant reaction mixture was stirred at RT for 16 h. The reaction mixture was diluted with water and extracted with ethyl acetate (2 xlOO mL). The 25 combined ethyl acetate layer was washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification by column chromatography (neutral alumina, 0.5% methanol in dichloromethane) afforded the title compound as a solid (500 mg) [600 mg of amide (2a) was recovered], 'H NMR (400MHz, DMSO-d6) 8 = 11.42 (br s, 1H), 9.96 (s, 1H), 7.92 - 7.77 (m, 2H), 7.40 (br d, ./=8.8 Hz, 2H), 7.32 (br s, 1H), 4.27 (q, ./=6.8 Hz, 2H), 2.90 (br s, 30 4H), 2.78 (br s, 2H), 2.44 - 2.29 (m, 7H), 2.15 (s, 3H), 1.56 (br dd, J=1.4, 14.4 Hz, 2H), 1.40 (br t, J=6.7 Hz, 3H), 0.89 (br t, 2 Hz, 3H), LCMS (M+H) =519.9. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -56 - EXAMPLE 10 2-(2-ethoxy-5-(4-methylpiperazin-l-ylsulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[l,2-f][1,2,4]triazine-6-carbaldehyde (1j) To a stirring solution of l-amino-N-(2-ethoxy-5-(4-methylpiperazin-l-ylsulfonyl)benzoyl)-4- 5 formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxamide (11) (1.5 g, 2.89 mmol) in t-butanol (30 vol), was added potassium hydroxide (2 g) and the resultant reaction mixture was heated to 100 °C for 2 days. The reaction mixture was cooled to RT and concentrated under reduced pressure, the obtained solid was purified by basic alumina by eluting with 0-0.5% methanol in dichloromethane to afford the title compound as a solid (500 mg) [SM was recovered by 2% 10 methanol in dichloromethane]. ’H NMR (400MHz, DMSO-d6) 8 = 11.78 (s, 1H), 10.12 (s, 1H), 7.94 - 7.76 (m, 2H), 7.39 (br d, .7=9.3 Hz, 1H), 4.28 - 4.16 (m, 2H), 4.09 (q, .7=5 1 Hz, 4H), 3.13 - 3.04 (m, 2H), 2.91 (br s, 4H), 2.68 (s, 3H), 2.41 - 2.27 (m, 4H), 2.09 (s, 3H), 1.65 (br dd, J=1.2, 14.7 Hz, 2H), 1.33 (brt, .7=6.7 Hz, 3H), 0.89 (br t,.7=7.2 Hz, 3H), Mass (M-H) =500.1. EXAMPLE 11 15 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-6-(hydroxymethyl)-5-methyl-7- propylpyrrolo[2,l-f][l,2,4]triazin-4(3H)-one (lb) To a stirring solution of 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4- oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Ij) (400 mg, 0.798 mmol) in methanol, was added sodium borohydride (75 mg, 1.996 mmol) and the resultant 20 reaction mixture was stirred at RT for 16 h. The reaction mixture was concentrated under reduced pressure and the obtained residue was diluted with dichloromcthane (30 mL) and washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification by washings with ether (3x 6 mL) afforded the title compound as a solid (320 mg). ’H NMR (400MHz, DMSO-d6) 8 = 11.39 (br s, 1H), 7.84 (br d, .7=8.8 Hz, 1H), 7.80 25 (d, .7=1.9 Hz, 1H), 7.38 (br d, .7=8.8 Hz, 1H), 4.66 (br t, .7=5.1 Hz, 1H), 4.40 (s, 2H), 4.21 (q, .7=6.8 Hz, 2H), 2.91 (br s, 4H), 2.79 (br t, .7=7.4 Hz, 2H), 2.44 (s, 3H), 2.36 (br s, 4H), 2.14 (s, 3H), 1.70 - 1.49 (m, 2H), 1.33 (br t, .7=7.0 Hz, 3H), 0.88 (t, .7=7.2 Hz, 3H), LCMS (M+H) =504.1, purity=97.2%. EXAMPLE 12 30 (2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f|[l,2,4]triazin-6-yl)methyl acetate (1c) CA 03001728 2018-04-11 WO 2017/085056 — 57 — PCT/EP2016/077720 A solution of 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-6-(hydroxymethyl)-5- methyl-7-propylpyrrolo[2,l-f][l,2,4]triazin-4(3H)-one (lb) (100 mg, 0.198 mmol) in acetic anhydride (0.5 mL) was stirred at RT for 16h. The reaction mixture was diluted with water (10 mL) and extracted with dichloromethane (3 xl5 mL). The combined dichloromethane layer was 5 washed with water (5-6 times), dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification was done by washings with ether (2x2 mL) to afford the title compound as a solid (70 mg). 'H NMR (400MHz, DMSO-d6) 8 = 11.52 (s, 1H), 7.94 - 7.72 (m, 2H), 7.39 (br d, ./=8.8 Hz, 1H), 5.07 (s, 2H), 4.22 (q, ,/=6.5 Hz, 2H), 3.05 - 2.70 (m, 6H), 2.45 (s, 3H), 2.37 (br s, 3H), 2.15 (s, 3H), 2.01 (s, 3H), 1.70 - 1.53 (m, 2H), 1.34 (br t, ./=6.7 Hz, 3H), 10 0.89 (br t, J=7.2 Hz, 3H), LCMS (M+H) =546.3, purity=99.5%. SCHEME 6: 15 EXAMPLE 13 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-6-(hydroxymethyl)-5-methyl-7- propylpyrrolo[2,l-f][l,2,4]triazin-4(3H)-one (12) CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -58 - To a stirring solution of 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4- oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Ij) (200 mg, 0.39 mol) in ethanol (5 mL), was added tosyl hydrazine (81.77 mg, 0.43 mmol) and the resultant reaction mixture was heated to 50 °C for 16 h. The reaction mixture was concentrated under reduced 5 pressure to afford the title compound as an off white solid (250 mg). LC-MS (M+H) =669.2, purity~94.3%. EXAMPLE 14 methyl 2-((2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo|2,l-f||l,2,4]triazin-6-yl)methoxy)acetate (13) 10 To a stirring solution of 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-6- (hydroxymethyl)-5-methyl-7-propylpyrrolo[2,l-f][l,2,4]triazin-4(3H)-one (12) (150 mg, 0.224 mmol) in dioxane (8 mL), were added potassium carbonate (108 mg, 0.784 mmol) and methylglycolate (81 mg, 0.448 mmol). The resulted mixture was heated in a microwave at 120°C for 1 h. The reaction mixture was concentrated and the obtained crude was purified by prep TLC 15 to afford the title compound as an off white solid (70 mg), 'll NMR (400MHz, DMSO-d6) 5 = 11.44 (s, 1H), 7.91 - 7.76 (m, 2H), 7.38 (d, .7=8.8 Hz, 1H), 4.51 (s, 2H), 4.21 (q, J=7.0 Hz, 2H), 4.13 (s, 2H), 3.68 (s, 3H), 2.91 (br s, 4H), 2.81 (br t, J=1.4 Hz, 2H), 2.44 (s, 3H), 2.40 - 2.27 (m, 4H), 2.15 (s, 3H), 1.61 (br dd, J=7A, 14.9 Hz, 2H), 1.33 (t, .7=6.7 Hz, 3H), 0.93 - 0.83 (m, 3H), Mass (M+H) =576.3. 20 EXAMPLE 15 2-((2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo|2,l-f|| 1,2,4]triazin-6-yl)methoxy)acetic acid (Ih) To a stirring solution of methyl 2-((2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2, 1-f][1,2,4]triazin-6-yl)methoxy)acetate (13) (25 25 mg, 0.043 mmol) in 2:1 ratio of tetrahydrofuran and water (1+ 0.5 mL), was added lithium hydroxide (4.9 mg, 0.13 mmol) and the resultant reaction mixture was stirred at RT for 3 h. The reaction mixture was concentrated and the residue was neutralized with saturated citric acid and then extracted with dichloromethane (2 x20 mL).The combined dichloromethane layer was washed with brine, dried over sodium sulfate and concentrated under reduced pressure. 30 Purification was carried out by prep TLC to afford the title compound as a pale green solid (10 mg). ’H NMR (400MHz, DMSO-d6) 8 = 11.37 (s, 2H), 7.97 - 7.71 (m, 2H), 7.38 (br d, .7=8.8 CA 03001728 2018-04-11 WO 2017/085056 -59- PCT/EP2016/077720 Hz, 1H), 4.51 (br s, 2H), 4.21 (q, .7=6.8 Hz, 2H), 3.62 (br s, 2H), 3.05 - 2.71 (m, 6H), 2.49 - 2.30 (m, 7H), 2.14 (s, 3H), 1.60 (br d, ,7=6.5 Hz, 2H), 1.33 (br t, .7=6.7 Hz, 3H), 0.86 (br d, .7=2.3 Hz, 3H), LCMS (M-H) =560.3, purity=97.8% EXAMPLE 16 5 2-((2-(2-ethoxy-5-((4-methylpiperazin-l-yI)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][1,2,4]triazin-6-yl)methoxy)-N-hydroxy-N-methylacetamide (li) To a stirring solution of 2-((2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl- 4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazin-6-yl)methoxy)acetic acid (Ih) (15 mg, 0.0267 mmol) in dimethylformamide (1 mL), was added HBTU (12 mg, 0.032 mmol) and 10 DIPEA (6.9 mg, 0.05 mmol). The mixture was then added with hydroxylamine hydrochloride (2.7 mg, 0.032 mmol) and the reaction mixture was stirred at RT for 2 h. The reaction mixture was quenched with ice water (5 mL) and extracted with ethyl acetate (2x10 mL). The combined ethyl acetate layer was washed with brine, dried over sodium sulfate and concentrated under reduced pressure. Purification by prep TLC afforded the title compound as a pale brown solid (5 15 mg). 'H NMR (400MHz, DMSO-d6) 3 = 11.45 (s, IH), 9.75 (br s, IH), 7.94 - 7.76 (m, 2H), 7.38 (d, J=8.8 Hz, IH), 4.51 (s, 2H), 4.28 - 4.05 (m, 4H), 3.10 (s, 3H), 2.91 (br s, 4H), 2.85 - 2.78 (m, 2H), 2.44 (s, 3H), 2.36 (br s, 4H), 2.14 (s, 3H), 1.60 (br dd, J=1.4, 14.4 Hz, 2H), 1.33 (t, J=1.Q Hz, 3H), 0.87 (t, J=7.4 Hz, 3H), LCMS (M-H) =560.3, purity=98.2%. 20 SCHEME 7: lj la EXAMPLE 17 (£)-2-(2-ethoxy-5-((4-methylpiperazin- -yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-31 ,4- dihydropyrrolo[2,1-f][1,2,4]triazine-6-carbaldehyde oxime 25 (la) CA 03001728 2018-04-11 WO 2017/085056 -60- PCT/EP2016/077720 To a stirring solution of 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4- oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Ij) (20 mg, 0.039 mmol) in pyridine (0.3 mL) was added hydroxylamine hydrochloride (3.7 mg, 0.05 mmol) and the reaction mixture was heated to 80 °C for 4 h. Purification was done by prep TLC to afford the 5 title compound as a white solid (10 mg^H NMR (400MHz, DMSO-d6) 8 = 11.57 (s, 1H), 10.95 (s, 1H), 8.21 (s, 1H), 7.93 - 7.77 (m, 2H), 7.39 (br d, ,7=8.8 Hz, 1H), 4.31 - 4.15 (m, 2H), 3.08 - 2.79 (m, 6H), 2.55 (br s, 3H), 2.37 (br s, 4H), 2.14 (s, 3H), 1.67-1.51 (m, 2H), 1.34 (br t, .7=6.7 Hz, 3H), 0.87 (br t, .7=7.2 Hz, 3H) LCMS (M-H) =517.1, purity=96.8%. 10 SCHEME 8: lg EXAMPLE 18 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- 15 dihydropyrrolo[2,l-f][l,2,4]triazine-6-carboxylic acid (Id) To a stirred suspension of Ij (200 mg) in acetone and water (1:1, 20 mL) was added a solution of KMnO4 in acetone and water (1:1, 20 mL) over a period of 30 min, subsequently while addition CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -61 - the pH of the reaction mixture (pH-8) was adjusted to pH-5 by using a buffer solution (IN KH2PO4 and IN HC1, pH-3.5). After completion of the addition the reaction mixture was stirred at RT for 2h. On completion, the reaction mixture was quenched with 10% aq sodium bisulfate solution and the mixture was concentrated to remove acetone at RT. The obtained aqueous 5 mixture was saturated with NaCl and filtered. The residual solid was stirred with 10% methanol in dichloromethane and filtered through celite. The filtrate was dried over anhydrous Na2SO4 and concentrated to afford compound Id (120 mg) as white solid. *H NMR (400MHz, DMSO-d6 + one drop of TFA) 8 = 7.96 - 7.88 (m, 2H), 7.43 (d, .7=9.4 Hz; 1H), 4.23 (q, J=7.0 Hz; 2H), 3.81 (br d, J=1 1.8 Hz; 2H), 3.49 (br d, J=11.8 Hz; 2H), 3.25 - 3.06 (m, 4H), 2.81 (s, 3H), 2.70 - 2.55 10 (m, 5H), 1.69 - 1.57 (m, 2H), 1.35 (t, J=6.9 Hz; 3H), 0.88 (t, .7=7.3 Hz; 3H), LCMS (M+H) = 518.1, purity=93.5%. EXAMPLE 19 methyl 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,l-f][1,2,4]triazine-6-carboxylate (If) 15 To a stirred solution of compound Id (80 mg) in methanol, thionyl chloridewas added slowly at 0 °C and the reaction mixture was refluxed for 16h. On completion, the reaction mixture was concentrated and purified by prep-HPLC to afford compound If (35 mg) as pale yellow solid. 1 H NMR (400MHz, CDCI3) 8 = 9.62 (s, 1H), 8.53 (d, J=2.4 Hz; 1H), 7.87 (dd, J=2.4, 8.9 Hz; 1H), 7.15 (d, .7=8.9 Hz; 1H), 4.34 (q, .7=7.1 Hz; 2H), 3.89 (s, 3H), 3.35 - 3.18 (m, 2H), 3.09 (br s, 3H), 20 2.76 (s, 2H), 2.50 (br s, 3H), 2.28 (s, 2H), 1.78 - 1.66 (m, 2H), 1.61 (t, .7=7.0 Hz; 3H), 0.98 (t, .7=7.4 Hz; 3H), LCMS (M+H) =532.1, purity=96.8%. EXAMPLE 20 N-(benzyloxy)-2-(2-ethoxy-5-((4-methylpiperazin-l-yi)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazine-6-carboxamide (1e) 25 To a stirred solution of compound If (100 mg) in DMF, HBTU (110 mg, 0.288 mmol) was added followed by DIPEA (0.2 mL, 1.152 mmol) at RT and stirred for 15 min. To this solution O-benzyl hydroxylamine hydrochloride (1.1 mmol) was added and the reaction mixture was stirred at RT for 6h. After completion, the reaction mixture was diluted with ice cold water and filtered. The obtained residue was washed with diethyl ether and dried to afford le (60 mg) as 30 white solid. LCMS (M+H) =623.3, purity=95.1%. CA 03001728 2018-04-11 WO 2017/085056 -62- PCT/EP2016/077720 EXAMPLE 21 2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-N-hydroxy-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carboxamide (1g) To a stirred solution of le (70 mg) in methanol (2 mL), Pd(OH)2 (20% w/w) was added and the 5 reaction mixture was hydrogenated under atmospheric pressure for Ih. After completion the reaction mixture was filtered through celite and the filtrate was concentrated. The crude material was purified by prep-TLC to afford 1g (14 mg) as pale yellow solid (which develops colored spot on TLC after standing at refrigerator temperature). 1H NMR (400MHz, DMSO-d6) 8 = 11.61 (s, IH), 10.55 (br s, IH), 8.99 (d, 7=1.9 Hz; IH), 7.93 - 7.70 (m, 2H), 7.39 (d, 7=8.9 Hz; 10 IH), 4.21 (q, 7=6.8 Hz; 2H), 3.38 (q, 7=7.0 Hz; 2H), 2.99 - 2.80 (m, 3H), 2.48 (s, 2H), 2.40 - 2.30 (m, 2H), 2.15 (s, IH), 1.60 (br dd, 7=7.4, 15.2 Hz; 2H), 1.33 (t, 7=6.9 Hz; 2H), 1.24 (br s, IH), 1.09 (t, 7=7.0 Hz; 2H), 0.85 (t, 7=7.3 Hz; 2H), LCMS (M+H) =533.1, purity=95%. EXAMPLE 22 Ethyl l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (14) CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -63 - To a stirred solution of 190 mg (0.92 mmol) ethyl 4-formyl-3-methyl-5-propyl-lH-pyrrole-2- carboxylate in 5 mL tert-butyl methyl ether 25 mg aliquat 336, 290 mg (5.3 mmol) NH4C1, 2.5 mL 30% aqueous NaOH and 2.5 mL 9% aqueous NH4C1 were added. During 20 min. 5.8 mL 9% NaOCl was added under vigorous stirring at room temperature. After 3 hours the reaction 5 mixture was extracted using ethyl acetate and brine, dried with magnesium sulfate and the solvent removed under reduced pressure to afford 210 mg (quantitative) the title compound as a white solid. ’H NMR (300MHz, DMSO-d6) 8 = 9.89 (s, 1H), 6.11 (s, 2H), 4.27 (q, .7=7.2 Hz; 2H), 2.98 - 2.86 (m, 2H), 2.48 (s, 3H), 1.64 - 1.48 (m, 2H), 1.36-1.27 (m, 3H), 1.24 (br s, 1H), 0.90 (t, ,7=7.3 Hz; 3H). LCMS (M+H) =239.1, purity=94%. 10 EXAMPLE 23 Ethyl 4-formyl-3-methyl-l-(2-propoxybenzamido)-5-propyl-lH-pyrrole-2-carboxylate (15) A solution of 200 mg (1 mmol) 2-propoxybenzoyl chloride in 2 mL dimethylformamide were added at 10 °C to 160 mg (1 mmol) Hiinig’s base in 2 mL dimethyl formamide. A solution of 210 mg (0.92 mmol) ethyl l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (14) 15 in 2 mL dimethylformamide was added and the reaction mixture was stirred at room temperature for 3 hours. The reaction mixture was extracted with ethyl acetate and washed with aqueous sodium carbonate, 0.5 N HC1 and brine. The organic layer was dried with magnesium sulfate and the solvent removed under reduced pressure to give the crude product as a gum. Chromatography om silica using cyclohexane/ethyl acetate 3/1 afforded 290 mg (79%) of the 20 title compound as a white solid. 'H NMR (500MHz, CDC13) 8 = 10.19 (s, 1H), 8.26 (dd, .7=2, 7 Hz; 1H), 7.54 (dd, .7=8, 7 Hz; 1H), 7.13 (t, .7=7 Hz; 1H), 7.07 (d, J= 8 Hz, 1H), 4.17 (t, J=7 Hz, 2H), 4.16 (t, J=7 Hz, 2 H), 2.79 (s, 3H), 3.18 (m, 2 H), 1.81-1.74(m, 4 H), 1.15 (t, J=7Hz, 3H), 1.00 (t, J=7 Hz, 4H). LCMS (M+H) =401.2, purity=92%. EXAMPLE 24 25 4-formyl-3-methyl-l-(2-propoxybenzamido)-5-propyl-lH-pyrrole-2-carboxylic acid (16) To a solution of 400 mg (1 mmol) ethyl 4-formyl-3-methyl-1-(2-propoxybenzamido)-5-propyl- lH-pyrrole-2-carboxylate (15) in 1.5 mL water, 1.5 mL methanol and 1.5 mL tetrahydrofuran was added 1.5 mL 1 M sodium hydroxide aqueous solution. The reaction mixture was stirred at 50 °C for 5 hours. Extraction with ethyl acetate, washed with brine, dried with magnesium 30 sulfate afforded after removal of solvent 370 mg (quantitative) the title compound as a white solid. CA 03001728 2018-04-11 _ _ WO 2017/085056 64 PCT/EP2016/077720 LCMS (M+H) =373.2, purity=96%. EXAMPLE 25 4-formyl-3-methyl-l-(2-propoxybenzamido)-5-propyl-lH-pyrrole-2-carboxamide (17) A solution of 420 mg (1.2 mmol) 4-formyl-3-methyl-l-(2-propoxybenzamido)-5-propyl-lH- 5 pyrrole-2-carboxylic acid (16), 96 mg (1.84 mmol) ammonium chloride, 224 mg (1.46 mmol) HBOT, 640 uL (3.7 mmol) N,N-diisopropylethylamin (Hunig’s base) in 16 mL dimethyl¬ formamide was treated with 296 mg (1.44 mmol) N,N’-dicyclohexylcarbodiimide (DCC). The reaction mixture was stirred for 5 hrs. at 50 °C. Work up with ethyl acetate, brine wash and drying of the organic phase with magnesium sulfate afforded 427 mg (96%) title compound as a 10 white solid. LCMS (M+H) =372.2, purity=93%. EXAMPLE 26 5-methyl-4-oxo-2-(2-propoxyphenyl)-7-propyl-3,4-dihydropyrrolo[2,l-f] [l,2,4]triazine-6- carbaldehyde (18) 600 mg (1.6 mmol) 4-formyl-3-methyl-l-(2-propoxybenzamido)-5-propyl-lH-pyrrole-2- 15 carboxamide (17) were dissolved in 60 mol polyethylene glycol (PEG 400). The solution was dried for 30 minutes at 90 °C and 8 mbar. 562 mg (5 mmol) potassium tert-butoxide were added and the reaction mixture was heated at 8 mbar for 1 hour at 140 °C. Dilution with water and extraction with ethyl acetate afforded the crude product as a white solid. Chromatography on silica using ethyl acetate/cyclohexane 7/3 afforded 490 mg (87%) title compound as a white 20 solid. ’H NMR (500MHz, CDC13) 8 = 10.18 (s, 1H), 10.09 (s, 1H), 8.19 (dd, J=2, 7 Hz; 1H), 7.50 (dd, J=8, 7 Hz; 1H), 7.13 (t, J=1 Hz; 1H), 7.06 (d, J= 8 Hz, 1H), 4.16 (t,J=7 Hz, 2H), 3.23 (t, J=7 Hz, 2 H), 2.79 (s, 3H), 2.04-1.95 (m, 2 H), 1.81-1.74(m, 2 H), 1.15 (t, J=7Hz, 3H), 1.00 (t, J=7 Hz, 4H). LCMS (M+H) =354.2, purity=97%. 25 CA 03001728 2018-04-11 WO 2017/085056 -65- PCT/EP2016/077720 SCHEME 10: 5 EXAMPLE 27 3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f| [1,2,4] triazin-2-yl)-4- propoxybenzene-l-sulfonyl chloride (19) 10 44 mg (0.12 mmol) 5-methyl-4-oxo-2-(2-propoxyphenyl)-7-propyl-3,4-dihydropyrrolo[2,l- d[l ,2,4] triazine-6-carbaldehyde (18) was treated at 0 °C with 250 uL (3.8 mmol) chlorosulfuric acid. After 2 hours at 0 °C the starting material was completely dissolved resulting in a deep red solution. The reaction mixture was quenched with ice and water and extracted with 20 mL methylene chloride. This extract was directly used for the conversion to the sulfonamide. 15 EXAMPLE 28 2-(5-((4-(2-hydroxyethyl)piperidin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f] [l,2,4]triazine-6-carbaldehyde (Ip) A solution of 0.12 mmol 3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][l,2,4]triazin-2-yl)-4-propoxybenzene-l-sulfonyl chloride (19) in 20 mL methylene chloride CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -66 - was treated at room temperature with a solution of 32 mg (0.25 mmol) 2-(piperidin-4-yl)ethanol and 64 mg (0.63 mmol) trimethylamine in 2 mL methylene chloride. After two hours at room temperature the reaction mixture was washed with diluted hydrochloric acid, dried with magnesium sulfate and concentrated. Chromatography on silica using cyclohexane / ethyl acetate 5 1/1 resulted in 61 mg (90%) title compound as a white solid. 'H NMR (500MHz, CDCh) 8 = 10.18 (s, 1 H), 9.89 (s, 1 H), 8.50 (d, J=3 Hz, 1 H), 7.86 (dd, J= 3, 9 Hz, 1H), 7.16 (d, J= 9 Hz, 1 H), 4.24 (t, J= 7 Hz, 2 H), 3.79 (d, J=12 Hz, 1 H), 3.65 (t, J=7 Hz, 2 H), 3.21 (t, J=8 Hz, 2 H), 2.78 (s, 3 H), 2.32 (t, J=10 Hz, 2 H), 1.80-1.15 (m, 11 H), 1.16 (t, J=8 Hz, 3 H), 0.99 (t, J=8 Hz, 3 H). LCMS (M+H) =545.2, purity=98%. 10 EXAMPLE 29 2-(l-((3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydro pyrrolo[2,l-f| [1,2,4]triazin-2-yl)-4- propoxyphenyl) sulfonyl) piperidin-4-yl)ethylnitrate (Iq) 68 mg (0.125 mmol) 2-(5-((4-(2-hydroxyethyl)piperidin-l-yl)sulfonyl)-2-propoxyphenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4] triazine-6-carbaldehyde (Ip) in 20 mL 15 methylene chloride was treated with 180 mg (0.69 mmol) triphenylphosphine and 125 mg (0.69 mmol) N-bromosuccinimide at room temperature. The reaction mixture was refluxed for 8 hours, after which TLC showed complete conversation to 2-(5-((4-(2-bromoethyl)piperidin-l- yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]- triazine-6-carbaldehyde (20). Work-up with methylene chloride, washed with brine, dried with 20 magnesium sulfate and removal of solvent under reduced pressure gave a colorless gum, which was dissolved in 10 mL acetonitrile. 190 mg (1.1 mmol) silver nitrate in 5 mL acetonitrile was added at room temperature. The reaction mixture was stirred for 3 days, worked up with ethyl acetate ad brine washed. The organic phase was dried with magnesium sulfate and the solvent removed under reduced pressure. Chromatography on silica using ethyl acetate / cyclohexane 2/3 25 gave 45 mg (61%) title compound as a white solid. *H NMR (500MHz, CDCh) 8 = 10.15 (s, 1 H), 10.09 (s, 1 H), 8.50 (d, J=2 Hz, 1 H), 7.85 (dd, J= 2, 9 Hz, 1H), 7.16 (d, J= 9 Hz, 1 H), 4.47 (t, J= 7 Hz, 2 H), 4.22 (t, J=7 Hz, 2 H), 3.83 (d, J=11 Hz, 2 H), 3.66 (m, 2 H), 3.04 (t, J=8 Hz, 2 H), 2.79 (s, 3H), 2.35-1.30 (m, 12 H), 1.16 (t, J=7 Hz, 3 H), 0.98 (t, J=7 Hz, 3 H). LCMS (M+H) =590.2, purity=96%. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -67 - EXAMPLE 30 (E)-2-(5-((4-(2-hydroxyethyl)piperidin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde oxime (1r) A solution of 40 mg Ip (0.07 mmol) in 10 mL ethanol was treated with 50% aqueous 5 hydroxylamine. After 4 hours at room temperature the reaction mixture was extracted with ethyl acetate, washed with brine, dried with magnesium sulfate and the solvent removed under reduced pressure. Chromatography on silica using cyclohexane / ethyl acetate 1/1 resulted in 35 mg (85%) title compound as a white solid. 'H NMR (500MHz, CDC13) 8 = 9.98 (s, 1 H), 8.42 (d, J=2 Hz, 1 H), 8.26 (s, 1 H), 7.82 (dd, J= 2, 9 Hz, 1H), 7.12 (d, J= 9 Hz, 1 H), 4.19 (t, J= 7 Hz, 10 2 H), 3.77 (d, J=11 Hz, 2 H), 3.65 (t, J=7 Hz, 2 H), 3.00 (t, J=8 Hz, 2 H), 2.57 (s, 3 H), 2.31 (t, J=11 Hz, 2 H), 2.10-1.30 (m, 11 H), 1.23 (t, J=7 Hz, 3 H), 0.95 (t, J=8 Hz, 3 H). LCMS (M+H) =560.3, purity=98%. SCHEME 11: 15 EXAMPLE 31 (E)-2-(l-((3-(6-((hydroxyimino)methyl)-5-metliyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][l,2,4]trlazin-2-yl)-4-propoxyphenyl) sulfonyl) piperidin-4-yl)ethyl nitrate (Iv) 20 25 mg (0.04 mmol) 2-(l-((3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- f][l,2,4]triazin-2-yl)-4-propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate (Iq) was dissolved in 20 mL ethanol. 0.5 mL 50% aqueous hydroxylamine was added at room temperature. After 3 hours at room temperature the reaction mixture was taken up in ethyl acetate and brine washed, dried over magnesium sulfate. The solvent was removed to give 22 mg (86%) title compound. 25 ’H NMR (500MHz, CDC13) 8 = 9.83 (s, 1 H), 8.50 (d, J=2 Hz, 1 H), 8.29 (s, 1 H), 7.85 (dd, J= 2, 9 Hz, 1H), 7.16 (d, J= 9 Hz, 1 H), 4.47 (t, J= 7 Hz, 2 H), 4.22 (t, J=7 Hz, 2 H), 3.83 (d, J=ll Hz, 2 H), 3.66 (m, 2 H), 3.04 (t, J=8 Hz, 2 H), 2.35-1.30 (m, 11 H), 1.16 (t, J=7 Hz, 3 H), 0.98 (t, J=7 Hz, 3 H). LCMS (M+H) =605.2, purity=92%. CA 03001728 2018-04-11 WO 2017/085056 -68- PCT/EP2016/077720 SCHEME 12: 5 EXAMPLE 32 5-methyl-2-(5-((4-methylpiperazin-l-yl)sulfonyl)-2-propoxyplienyl)-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][1,2,4]triazine-6-carbaldehyde (11) Following the procedure for Ip starting from 40 mg (0.11 mmol) 5-methyl-4-oxo-2-(2- propoxyphenyl)-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (19) and using 10 87 mg (0.75 mmol) 1-methylpiperazine gave after chromatography on silica using ethyl acetate / methanol (98/2) 46 mg (79%) title compound as a white solid. *H NMR (500MHz, CDCI3) 8 = 9.68 (s, 1 H), 8.51 (d, J=2 Hz, 1 H), 7.83 (dd, J= 2, 9 Hz, 1H), 7.16 (d, J= 9 Hz, 1 H), 4.65 (s, 2 H), 4.23 (t, J=7 Hz, 2 H), 3.64 (s, 2 H), 3.20-2.58 (m, 8 H), 3.14 (s, 3 H), 2.95 (t, J=8 Hz, 2 H), 2.58 (s, 3 H), 2.00 (m, 2 H), 1.72 (m, 2 H), 1.16 (t, J=7 Hz, 3 H), 0.97 (t, J=7 Hz, 3 H). LCMS 15 (M+H) =516.2, purity=96%. CA 03001728 2018-04-11 WO 2017/085056 -69- PCT/EP2016/077720 EXAMPLE 33 2-(5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)-2-propoxyphenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f| |1,2,4|triazine-6-carbaldehyde (Im) Following the procedure for Ip starting from 275 mg (0.78 mmol) 5-methyl-4-oxo-2-(2- 5 propoxyphenyl)-7-propyl-3,4- dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (18) and using 0.5 mL (4.0 mmol) 2- (piperazin-l-yl)ethanol gave after chromatography on silica using ethyl acetate / cyclohexane / methanol (40/60/3) 383 mg (90%) title compound as a colorless gum. 'H NMR (400MHz, CDC13) 8 = 10.19 (s,lH), 9.68 (s, 1 H), 8.52 (d, J-2 Hz, 1 H), 7.89 (dd, J= 2, 9 Hz, 1H), 7.18 (d, J= 9 Hz, 1 H), 4.39-4.34 (m, 2 H), 3.59 (m, 2H), 3.23 (t, J=7 Hz, 2 10 H), 3.12 (brs, 4 H), 2.79 (s, 3H), 2.64-2.58 (m, 6 H), 1.79-1.72 (m, 4 H), 1.62 (t, J=7 Hz, 3 H), 0.99 (t, J=7 Hz, 3 H). LCMS (M+H) =546.2, purity=93%. NaBH4 EtOH 15 EXAMPLE 34: 2-(5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)-2-propoxyphenyl)-6-(hydroxymethyl)-5- methyl-7-propylpyrrolo[2,l-f|[1,2,4]triazin-4(3H)-one (In) To a solution of 310 mg (0.57 mmol) 2-(5-((4-(2- hydroxyethyl)piperazin-l-yl)sulfonyl)-2- propoxyphenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo [2,l-f][l,2,4]triazine-6-carbalde- 20 hyde (Im) in 35 mL ethanol was added 150 mg (4 mmol) sodium borohydride. The reaction mixture was stirred over night at room temperature treated with 5 mL acetone and stirred for one hour. The reaction mixture was concentrated under reduced pressure, the residue taken up in ethyl acetate and the organic phase was washed with brine and dried with magnesium sulfate. Chromatography on silica using ethyl acetate / cyclohexane / methanol (40/60/4) gave 245 mg 25 (79%) title compound as a white solid. . 'H NMR (400MHz, CDCI3) 8 = 9.68 (s, 1 H), 8.52 (d, J=2 Hz, 1 H), 7.89 (dd, J= 2, 9 Hz, 1H), 7.18 (d, J= 9 Hz, 1 H), 4.79 (s, 2 H), 4.39-4.34 (m, 2 H), CA 03001728 2018-04-11 WO 2017/085056 — 70 — PCT/EP2016/077720 3.59 (m, 2H), 3.23 (t, J=7 Hz, 2 H), 3.12 (brs, 4 H), 2.79 (s, 3H), 2.64-2.58 (m, 6 H), 1.79-1.72 (m, 4 H), 1.62 (t, J=7 Hz, 3 H), 0.99 (t, J=7 Hz, 3 H). LCMS (M+H) =548.2, purity=97%. SCHEME 14: ^Li o -80° C 5 lj Is EXAMPLE 35 Ethyl-3-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl) phenyl)-5-methyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,l-f| [l,2,4]triazin-6-yl)-3-hydroxypropanoate (Is) 10 To a solution of 420 uL (3 mmol) diisopropylamine in 15 mL tetrahydrofuran at -80 °C was added 1.88 mL butyllithium (3 mmol) in hexane. After 10 minutes 390 uL (4 mmol) ethyl acetate was added. 118 mg (0.234 mmol) 2-(2-ethoxy-5-((4-methylpiperazin-l- yl)sulfonyl)phenyl)-5-methyl-4- oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6- carbaldehyde (lj) in 5 mL tetrahydrofuran was added after 10 minutes and the reaction mixture 15 was kept at -80 °C for 2 hours. Work up with ethyl acetate and brine, magnesium sulfate drying and removal of the solvent under reduced pressure gave a colorless gum. Chromatography on silica wit ethyl acetate / ethanol (5/1) gave 115 mg (83%) of the title compound as a white solid. ’H NMR (500MHz, CDC13) 8 = 9.94 (s, 1 H), 8.34 (d, J=2 Hz, 1 H), 7.77 (dd, J= 2, 8.5 Hz, 1H), 7.12 (d, J= 8.5 Hz, 1 H), 5.31 (m, 1 H), 4.34-4.30 (m, 2 H), 4.21-4.16 (m, 2 H), 3.05-2.90 (m, 20 4H), 2.63-2.48 (m, 8 H), 2.59 (s, 3 H) (s, 3 H), 2.26 (s, 3 H), 1.57 (t, J=7Hz, 3H), 1.28 (t, J=7.5 Hz, 3 H), 0.98 (t, J=7 Hz, 3 H). LCMS (M+H) =590.2, purity=95%. Is It GA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -71 - EXAMPLE 36 3-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][l,2,4]triazin-6-yl)-N,3-dihydroxy propanamide (It) 50 mg (0.085 mmol) ethyl 2-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyl)-5- 5 methyl-4-oxo-7-propyl-3,4-dihydro pyrrolo[2,l-f][1,2,4]triazin-6-yl)-2-hydroxyacetate (Is) was dissolved in 5 mL ethanol. 1.5 mL aqueous 50% hydrazine hydrate was added at room temperature and the reaction mixture was stirred at 35 °C for four days. Solvent was partially removed under reduced pressure, and worked up using ethyl acetate and brine. The organic phase was dried with magnesium sulfate and the solvent removed under reduced pressure. 10 Chromatography on silica with ethyl acetate / methanol (1/2) gave 20 mg (41%) title compound as a colorless solid. JH NMR (500MHz, CDC13) 5 = 13.2 (s, 1 H), 9.73 (s, 1 H), 8.49 (s, 1 H), 7.53 (m, 1H), 7.15 (m, 1 H), 5.30 (m, 1 H), 4.00-3.90 (m, 2 H), 3.07-2.80 (m, 4 H), 2.60-2.40 (m, 8H), 2.60 (s, 3 H) (s, 3 H), 2.30 (s, 3 H), 1.33 (t, J=7Hz, 3H), 0.88 (t, J=7 Hz, 3 H). LCMS (M+H) =577.2, purity=92%. 15 NH2OMe PEG 400 100 °C EXAMPLE 37 (E)-5-methyl-2-(5-((4-methylpiperazin-l-yl)sulfonyl)-2-propoxyphenyl)-4-oxo-7-propyl-3,4- 20 dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde O-methyl oxime (lo) 30 mg (0.06 mmol) 5- methyl-2-(5-((4-methylpiperazin-l-yl)sulfonyl)-2-propoxyphenyl)-4-oxo- 7-propyl-3,4- dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde, 92 mg (1.1 mmol) methylhydroxylamine hydrochloride and 46 mg (1.15 mmol) sodium hydroxide in 4.5 mL PEG- 400 were heated for 8 hours to 100 °C. Work up with ethyl acetate and aqueous sodium 25 carbonate solution, drying of the organic phase with magnesium carbonate and removal of the solvent under reduced pressure gave 19 mg (60%) title compound. 'H NMR (500MHz, CDCI3) 5 = 9.81 (s, 1 H), 8.45 (d, J=2 Hz, 1 H), 8.20 (s, 1 H), 7.85 (dd, J= 2, 9 Hz, 1H), 7.14 (d, J= 9 Hz, CA 03001728 2018-04-11 WO 2017/085056 — 72 — PCT/EP2016/077720 1 H), 3.96 (s, 3 H), 3.73-3.59 (m, 2 H), 3.09-3.00 (m, 4 H), 2.63 (s, 3 H), 2.53-2.50 (m, 4 H), 2.29 (s, 3 H), 1.72-1.60 (m, 4 H), 0.98 (t, J=7 Hz, 3 H), 0.95 (t, J=8 Hz, 3 H). LCMS (M+H) =545.2, purity=89%. 5 SCHEME 17: lu EXAMPLE 38 3-(2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl)phenyI)-5-methyl-4-oxo-7-propyI-3,4- dihydropyrrolo[2,l-f][l,2,4]triazin-6-yl)-3-hydroxy propanenitrile (lu) 10 To a solution of 420 uL (3 mmol) diisopropylamine in 15 mL tetrahydrofuran 1.88 mL butyllithium was added at -80 °C. After 15 minutes 0.5 mL acetonitrile were added and the solution stirred for 15 minutes at -80 °C. 90 mg (0.18 mmol) 2-(2-ethoxy-5-((4-methylpiperazin- l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,1-f][ ,2,4]triazine-6-carb-1 aldehyde (Ij) was added in 5 mL tetrahydrofuran was added and the reaction mixture was 15 warmed up to 0 °C. Work up with ethyl acetate and chromatography on silica using ethyl acetate / aceton (2/1) gave 60 mg (60%) title compound as a white solid. 'H NMR (500MHz, CDCI3) 8 = 9.90 (s, 1H), 8.32 (d, J=2 Hz; 1H), 7.75 (dd, J=A, 9 Hz; 1H), 7.13 (d, J=9 Hz; 1H), 5.19 (t, J= 8 Hz, 1H), 4.35 (m, 2H), 3.00-2.70 (m, 8 H), 2.47 (s, 3H), 2.26 (s, 3 H), 1.69-1.60 (m, 2 H), 1.61 (t, J=7Hz, 3H), 0.98 (t, J=7 Hz, 3H). LCMS (M+H) =543.2, purity=92%. 20 NaBH4 EtOH Is Ik CA 03001728 2018-04-11 WO 2017/085056 -73- PCT/EP2016/077720 EXAMPLE 39 6-(l,3-dihydroxypropyl)-2-(2-ethoxy-5-((4-methylpiperazin-l-yl)sulfonyl) phenyl)-5- methyl-7-propylpyrrolo[2,l-f][l,2,4]triazin-4(3H) -one (Ik) To a solution of 45 mg (0.08 mmol) ethyl 3-(2-(2- ethoxy-5-((4-methylpiperazin-l- 5 yl)sulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4] triazin-6-yl)-3- hydroxypropanoate (Is) was added 380 mg (10 mmol) sodium borohydride. The reaction mixture was heated to reflux for 12 hours. At room temperature 3 mL action was added and the reaction mixture was warmed up to 50 °C for 30 minutes. Standard work up using ethyl acetate and aqueous sodium carbonate afforded after chromatography on silica with ethyl acetate / 10 methanol (3/1) 15 mg title compound as a colorless resin. *H NMR (500MHz, CDCI3) 3 = 9.73 (s, 1 H), 8.41 (d, J=2 Hz, 1 H), 7.78 (dd, J= 2, 9 Hz, 1H), 7.13 (d, J= 9 Hz, 1 H), 5.13 (dd, J= 4, 10 Hz, 1 H), 3.89 (m, 2 H), 3.07-2.80 (m, 4 H), 2.55 (s, 3 H) (s, 3 H), 2.27 (s, 3 H), 1.70-1.30 (m, 4 H), 1.58 (t, J=7 Hz, 3 H), 0.98 (t, J=7 Hz, 3 H). LCMS (M+H) =548.3, purity=92%. 15 SCHEME 19: CA 03001728 2018-04-11 _ _ WO 2017/085056 74 PCT/EP2016/077720 EXAMPLE 40: 5-((4-(2-(Benzoyloxy)ethyl)piperazin-l-yl)sulfonyl)-2-ethoxybenzoic acid (21) A solution of 5-(chlorosulfonyl)-2-ethoxybenzoic acid (9) (5.66 g, 21.43 mmol) in dichloromethane (50 mL) was added to a cooled solution of 2-(Piperazin-l-yl)ethyl benzoate 5 (5.54 g, 23.67 mmol) and triethylamine (8.8mL, 64.53 mmol) in dichloromethane (100 mL) at 0°C. The resultant reaction mixture was stirred at RT for 16h. On completion, the reaction mixture was concentrated. The obtained residue was acidified with 5% aqueous citric acid solution and extracted with ethyl acetate (3x200mL). The combined ethyl acetate layers was washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated to afford the 10 title 21 (9.7g) as brown solid. ’H NMR (400MHz, CDC13) 8 = 8.48 (br d, ,/=2.4 Hz, 1H), 7.98 (br d, .7=8.3 Hz, 2H), 7.91 (dd, J=2.2, 8.6 Hz, 1H), 7.59 - 7.53 (m, 1H), 7.47 - 7.39 (m, 2H), 7.18 - 7. 12 (m, 1H), 4.45 (br t, ,7=5.6 Hz, 2H), 4.41 - 4.33 (m, 2H), 3.12 (br s, 4H), 2.89 (br t, J=4.9 Hz, 2H), 2.80 (br s, 4H), 1.59 (dt, J=2J, 7.0 Hz, 3H). LCMS (M+H) =463.3, purity~78%. EXAMPLE 41 15 Ethyl-l-(5-((4-(2-(benzoyloxy)ethyl)piperazin-l-yl)sulfonyl)-2-ethoxybenzamido)-4-formyl- 3-me+hvi-5-propvl- 1 77-pvrrole-2-carboxyiate (22) To a stirring solution of 5-((4-(2-(Benzoyloxy)ethyl)piperazin-l-yl)sulfonyl)-2-ethoxybenzoic acid (21) (3.5g, 7.55 mmol) in DMF (35 mL), was added TBTU (4.85 g, 15.11 mmol) and diisopropylethyl amine (3.96 mL, 22.67mmol) to this 14 (2.15 g, 9.07 mmol) was added and the 20 resultant reaction mixture was stirred at RT for 16 h. The reaction mixture was diluted with water and extracted with ethyl acetate (3 x200 mL). The combined ethyl acetate layer was washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification by column chromatography (100-200 mesh silica gel, 1-3% methanol in dichloromethane as eluent) afforded the title compound 22 (3.8g) as brown gum. 'H NMR 25 (300MHz, DMSO-d6) 8 = 11.43 (s, 1H), 10.00 (s, 1H), 7.94 - 7.80 (m, 4H), 7.68 - 7.59 (m, 1H), 7.54 - 7.46 (m, 2H), 7.42 (d, ,7=8.8 Hz, 1H), 4.46 - 4.24 (m, 4H), 4.17 (q, .7=7.2 Hz, 2H), 2.97 - 2.90 (m, 4H), 2.89 (s, 11H), 2.73 (s, 8H), 2.69 (s, 3H), 2.59 (br s, 2H), 2.54 (s, 3H), 1.58 (br dd, ,7=7.3, 15.0 Hz, 2H), 1.41 (t, .7=7.0 Hz, 3H), 1.16 (t, ,7=7.2 Hz, 3H), 0.90 (t, ,7=7.3 Hz, 3H), LC¬ MS (M+H) =684.0, purity~85%. CA 03001728 2018-04-11 WO 2017/085056 — 75 — PCT/EP2016/077720 EXAMPLE 42 l-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)benzamido)-4-formyl-3-methyl- 5-propyl-177-pyrrole-2-carboxylic acid (23) To a solution of 22 (10.6g, 15.54 mmol) in MeOH (100 mL) and H2O (100 ml), NaOH (10 g, 5 w/w) was added and stirred at 60°C for 6h. On completion, the reaction mixture was concentrated; the obtained aqueous residue was acidified with IN HCL The obtained solid was filtered, dried and washed with Et2O (3x 20 mL) to afford 23 (4.1 g) as white solid. NMR (300MHz, DMSO-d6) 8 = 11.48 (s, 1H), 10.00 (s, 1H), 7.91 - 7.78 (m, 2H), 7.41 (d, .7=8.8 Hz, 1H), 4.27 (q, J=7.0 Hz, 2H), 3.44 (br s, 2H), 2.88 (br s, 6H), 2.54 (s, 3H), 2.39 (br d, .7=12.1 Hz, 10 2H), 1.68 - 1.50 (m, 2H), 1.40 (t, .7=7.0 Hz, 3H), 0.90 (t, ,7=7.3 Hz, 3H). LC-MS (M-H) =551.4, purity-9 1%. EXAMPLE 43 l-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)benzamido)-4-formyl-3-methyl- 5-propyl-177-pyrrole-2-carboxamide (24) 15 To a stirring solution of 23 (1.68g, 3.054 mmol) in DMF (8 mL), TBTU (1.96 g, 6.10 mmol) and DIPEA (2.9 mL, 15.27 mmol) was added and stirred at RT for 30min. To the obtained reaction mixture NH4C1 (0.5 g, 9.16 mmol) was added and the mixture was stirred at RT for 16h. On completion, the reaction mixture was quenched with water (130 mL) and extracted with EtOAc (2x100 mL). The combined organic layer was washed with water (2x40 mL), brine (30 mL), 20 dried over anhydrous Na2SO4 and concentrated. The obtained crude was purified by silica gel (230-400 mesh) column chromatography using 10% Methanol in dichloromethane as eluent to afford the title compound 24 (2 g) as white solid. 'H NMR (400MHz, CDC13) 8 = 10.80 (br s, 1H), 10.04 (s, 1H), 8.50 (d, .7=2.4 Hz, 1H), 7.89 (dd, .7=2.4, 8.8 Hz, 1H), 7.15 (d, .7=8.8 Hz, 1H), 5.73 (br s, 2H), 4.40 (q, .7=7.2 Hz, 2H), 3.64 (t, .7=5.4 Hz, 2H), 3.10 (br s, 4H), 2.70 (br t, .7=4.6 25 Hz, 4H), 2.67 - 2.61 (m, 2H), 2.56 (s, 3H), 1.65 (t, .7=6.8 Hz, 3H), 0.97 (t, .7=7.3 Hz, 3H). LCMS (M+H) =550.4, purity-96.5%. EXAMPLE 44: 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Iw) 30 A solution of 24 (200 mg, 0.36 mmol) in absolute ethanol (5 mL) was added IM aqueous KOH solution ( 5 mL) and stirred at 95°C in a sealed tube for 72h. The reaction mixture was CA 03001728 2018-04-11 WO 2017/085056 -76- PCT/EP2016/077720 concentrated completely under reduced pressure. The crude was added water (5 mL) and stirred at RT for 10 min; the resulted solid was filtered, washed with diethyl ether (3x5 mL) and dried to afford the title Iw (110 mg) as brown solid. 'H NMR (400MHz, CDC13) 8 = 10.19 (s, 1H), 9.69 (s, 1H), 8.53 (d, ./=2.4Hz, 1H), 7.89 (dd, J=2.4Hz, 8.8Hz; 1H), 7.18 (d, J=8.8Hz,lH), 4.39- 5 4.34 (m, 2H), 3.59 (m, IH), 3.22 (t, ,/=7.2Hz, 2H), 3.11 (brs, 4H), 2.64-2.57 (m, 6H), 1.79-1.73 (m, 2H), 1.62 (t t, ,/=7.2 Hz, 3H), 0.99 (t, ,/=7.2 Hz, 3H), LCMS (M+H) =532.4, purity~99%. 10 EXAMPLE 45 (£)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde oxime (lx) To a stirring solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- 15 methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Iw) (80 mg, 0.15 mmol) in Ethanol (3 mL) and water (0.5 mL) was added hydroxylamine hydrochloride (26.17 mg, 0.376 mmol) and the reaction mixture was heated to 85 °C for 5h. On completion, the reaction mixture was concentrated under reduced pressure. The obtained residue was stirred in water (1 mL), filtered, washed with diethyl ether (2x3 mL) and dried to afford lx (60 mg) as a 20 white solid. ’HNMR (400MHz, DMSO-d6) 8 = 11.62 (s, 1H), 10.95 (s, 1H), 9.72 (brs, 1H), 8.21 (s, 1H), 7.91-7.89 (m, 2H), 7.44 (d, J=8.8Hz; 1H), 5.31 (br, 1H), 4.26-4.21 (m, 2H), 3.77-3.69 (m, 4H), 3.54 (br, 2H), 3.19 (m, 4H), 2.96-2.92 (m, 2H), 2.79-2.73 (m, 2H), 2.55 (s, 3H), 1.63- 1.56 (m, 2H), 1.35 (t, J=6.8Hz, 3H), 0.88 (t, ./=7.6 Hz, 3H), LCMS (M+H) =547.8, purity~93.6% (mixture of syn & anti isomers). 25 CA 03001728 2018-04-11 WO 2017/085056 -77- PCT/EP2016/077720 NH2OCH3xHC1, EtOH h2o EXAMPLE 46 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-inethyl-4-oxo-7- 5 propyl-3,4-dihydropyrrolo|2,l-f]1 1 ,2,4]triazine-6-carbaldehyde O-methyloxime (ly) To a stirring solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Iw) (80 mg, 0.15 mmol) in ethanol (3 mL) and water (0.5 mL) was added methoxylamine hydrochloride (31.5mg, 0.376 mmol) and the reaction mixture was heated to 85 °C for 4 h. On completion, the 10 reaction mixture was concentrated under reduced pressure. The obtained residue was stirred in water (1 mL), filtered, washed with diethyl ether (2><3 mL) and dried to afford the title compound ly (58 mg) as a white solid. ’H NMR (400MHz, CDC13) 5 = 8.23 (s, 1H), 8.06 (dd, J=2, 8.8Hz, 1H), 7.18 (d, J=8.8Hz,lH), 4.30 (q, J=6.8Hz,2H), 3.67-3.64 (m, 2H), 3.12-3.03 (m, 5H), 2.76 (br, 4H), 2.66-2.64 (m, 2H, 2.61 (s, 3H), 1.73-1.67 (m, 2H), 1.48 (t, J=6.8 Hz, 3H), 15 0.95 (t, ,/=7.2 Hz, 3H), LCMS (M+H) =561.37, purity~96%. SCHEME 22: 20 EXAMPLE 47 (£)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde O-(2-hydroxyethyl) oxime (Iz) CA 03001728 2018-04-11 WO 2017/085056 -78- PCT/EP2016/077720 To a stirring solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Iw) (50 mg, 0.094 mmol) in ethanol (2 mL) and water (0.5 mL) was added 2-Aminoxyethanol (25.4 mg, 0.33 mmol) and the reaction mixture was heated to 85 °C for 16 h. On completion, the reaction 5 mixture was concentrated under reduced pressure. The obtained residue was stirred in water (1 mL), filtered, washed with diethyl ether (2x3 mL). The obtained crude was further purified by PREP TLC using 25% Acetone in dichloromethane as eluent to afford the title Iz (33 mg) as a white solid ’H NMR (400MHz, CDC13) 8 = 11.62 (s, 1H), 8.30 (s, 1H), 7.86-7.82 (m, 2H), 7.39 (d, J=8.8Hz,lH), 4.70-4.67 (m, 1H), 4.36-4.35 (m, 1H), 4.24-4.19 (m, 2H), 4.09-4.07 (m, 2H), 10 3.68-3.67 (m, 2H), 3.44-3.41 (m, 2H), 2.96-2.88 (m, 6H), 2.55 (s, 3H), 2.37-2.34 (m, 2H), 1 .62- 1.56 (m, 2H), 1.33 (t, ,/=7.2Hz, 3H), 0.88 (t, ,/=7.6 Hz, 3H), LCMS (M+H) =591.37, purity~95.7%. SCHEME 23: Zn, AcOH MED CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -79 - EXAMPLE 48 MethyI-3-ethvl-4-lormyl-l -hydroxy-5-propyl- 1 //-pyrrole-2-carboxylate (25) (Z)-ethyl 2-(hydroxyimino)-3-oxopentanoate (35 g, 219.99 mmol), (E)-hex-2-enal (50.9 mL, 5 439.9 mmol) in toluene, was added di-isopropylamine (6.2 mL, 43.99 mmol) and the resultant reaction mixture was stirred at RT for 16h. The reaction mixture was quenched with aqueous Ammonium chloride solution (300 mL) and extracted with dichloromethane (2x300 mL). The combined dichloromethane layer was washed with brine and dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification by column chromatography (100- 10 200 mesh silica gel, 0-5% ethyl acetate in pet ether as eluent) afforded the title compound 25 (31 g) as gum. '11 NMR (400MHz, CDC13) 5 = 12.27 (br s, 1H), 9.94 (s, 1H), 3.97 (s, 3H), 3.02 (q, .7=7.3 Hz, 2H), 2.98 - 2.92 (m, 2H), 1.77 - 1.63 (m, 2H), 1.19 (t, .7=7.3 Hz, 3H), 0.97 (t, J=7.3 Hz, 3H), Mass (M+H) =240.2. 15 EXAMPLE 49 Methyl-3-ethyl-4-formyl-5-propyl-l/7-pyrrole-2-carboxylate (26) To a stirring solution of ethyl 4-formyl-l-hydroxy-3-ethyl-5-propyl-lH-pyrrole-2-carboxylate (25) (10g, 41.84 mmol) in dichloromethane (800 mL) and acetic acid (40 mL), zinc dust (27.4 g, 418.41 mmol) was added in portions and the resultant reaction mixture was stirred at RT for 8h. 20 The reaction mixture was filtered and the filtrate was concentrated under reduced pressure. Purification of the crude by column chromatography (100-200 mesh silica gel, 5% methanol in dichloromethane as eluent) afforded the title compound 26 (7.5 g) as solid. [SM was recovered by eluting with 5% ethyl acetate in pet ether as eluent]. ’H NMR (400MHz, CDC13) 6 = 10.01 (s, 1H), 9.00 (br s, 1H), 3.88 (s, 3H), 3.07 (q, .7=7.7 Hz, 2H), 2.91 (t, .7=7.6 Hz, 2H), 1.76 - 1.65 (m, 25 2H), 1.20 (t, .7=7.3 Hz, 3H), 0.99 (t, .7=7.3 Hz, 3H), LCMS (M+H) =224.3, purity~96%. EXAMPLE 50 Methyl-l-amino-3-ethyl-4-formyl-5-propyl-177-pyrrole-2-carboxylate (27) To a stirring solution of 26 (5 g, 22.42 mmol), in DMF (50 mL) at 0°C, NaOH (4.48g, 112.1 mmol) was added in portions. After addition the mixture was stirred at 0°C for Ih before cooled CA 03001728 2018-04-11 WO 2017/085056 -80- PCT/EP2016/077720 it to -20°C. The obtained mixture was treated with chloramine solution in MTBE (freshly prepared from NH4CI (15 g) in MTBE (400 mL) added 30% aqueous ammonia (30 mL) and cooled to -20°C. To the mixture sodium hypochlorite solution (120 mL, commercial grade in water) was added drop wise. After addition the reaction mixture was stirred at -20°C for Ih. The 5 MTBE layer was separated from the biphasic mixture) was added slowly for 20 min. After addition, the reaction mixture was allowed to stir at RT for 3h. On completion, the reaction mixture was quenched with water and separated the organic layer. The MTBE layer was washed with 5% sodium thiosulfate solution and water (100 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure to afford the title compound 27 (5.2 g) as brown solid. 10 ’H NMR (400MHz, CDC13) 6 = 9.97 (s, IH), 5.39 (s, 2H), 3.89 (s, 3H), 3.09 - 2.96 (m, 4H), 1.68 - 1.60 (m, 2H), 1.21-1.12 (m, 3H), 0.98 (t, ,/=7.3 Hz, 3H), LCMS (M+H) =239.1, purity-81%. SCHEME 24: CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -81 - EXAMPLE 51 Methyl-l-(5-((4-(2-(benzoyloxy)ethyI)piperazin-l-yl)sulfonyl)-2-ethoxybenzamido)-3-ethyl- 4-formyl-5-propyl-ljH-pyrrole-2-carboxylate (28) 5 To a stirring solution of 5-((4-(2-(Benzoyloxy)ethyl)piperazin-l-yl)sulfonyl)-2-ethoxybenzoic acid (21) (9.7g, 20.99 mmol) in DMF (90 mL), was added HBTU (13.42 g, 41.9 mmol) and diisopropyl ethyl amine (10.8 mL, 62.9 mmol) to this 27 (5.86 g, 24.77 mmol) was added and the resultant reaction mixture was stirred at RT for 16 h. The reaction mixture was diluted with water and extracted with ethyl acetate (2 x200 mL). The combined ethyl acetate layer was 10 washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. Purification by column chromatography (100-200 mesh silica gel, 0.5% methanol in dichloromethane as eluent) afforded the title compound 28 (9.5g) as brown solid. 1H NMR (400MHz, CDC13) 8 = 10.41 (s, 1H), 10.06 (s, 1H), 8.57 (d, ,7=2.4 Hz, 1H), 8.01 - 7.97 (m, 2H), 7.93 (dd, .7=2.4, 8.3 Hz, 1H), 7.60 - 7.52 (m, 1H), 7.47 - 7.38 (m, 2H), 7.18 (d, .7=8.8 Hz, 1H), 15 4.49 - 4.33 (m, 4H), 3.77 (s, 3H), 3.06 (br s, 5H), 2.78 (br t, ,7=5.6 Hz, 1H), 2.67 (br s, 4H), 1.71 - 1.59 (m, 4H), 1.22 (t, ./7.6 Hz, 2H), 0.96 (t, ./7.3 Hz, 3H), LCMS (M+H) =685.3, purity-80%. EXAMPLE 52 l-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)benzamido)-3-ethyl-4-formyl-5- 20 propyl- l//-pyrrole-2-carboxylie acid (29) To a solution of 28 (9.5g, 13.9 mmol) in MeOH (100 mL) and H2O (100 mL), NaOH (9.5g, w/w) was added and stirred at 60°C for 3h. On completion, the reaction mixture was concentrated; the obtained aqueous residue was acidified with IN HC1. The obtained solid was filtered, dried and washed with Et2O (3x 10 mL) to afford 29 (6 g) as white solid, ’ll NMR 25 (400MHz, DMSO-d6) 8 = 9.96 (s, 1H), 7.91 (d, 7=2.4 Hz, 1H), 7.82 (dd, 7=2.4, 8.8 Hz, 1H), 7.39 (d, 7=8.8 Hz, 1H), 4.27 (q, 7=6.8 Hz, 2H), 3.42 (br t, 7=6.1 Hz, 4H), 3.06 (q, 7=7.2 Hz, 2H), 2.93 - 2.75 (m, 6H), 2.36 (t, 7=6.1 Hz, 2H), 1.58 (qd, 7=7.5, 15.1 Hz, 2H), 1.40 (t, 7=7.1 Hz, 3H), 1.10 (t, 7=7.3 Hz, 3H), 0.89 (t, 7=7.3 Hz, 3H), LC-MS (M+H) =565.5, purity-88.2%. CA 03001728 2018-04-11 WO 2017/085056 -82- PCT/EP2016/077720 EXAMPLE 53 l-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)benzamido)-3-ethyl-4-formyl-5- propyl-l.H-pyrrole-2-carboxamide (30) To a stirring solution of 29 (6g, 10.65 mmol) in DMF (60 mL), TBTU (6.84 g, 21.31 mmol) and 5 DIPEA (9.46 mL, 31.95 mmol) was added and stirred at RT for 30min. To the obtained reaction mixture NH4CI (1.14g, 21.31 mmol) was added and the mixture was stirred at RT for 16h. On completion, the reaction mixture was quenched with water (200 mL) and extracted with EtoAc (2x100 mL). The combined organic layer was washed with water 2x50 mL), brine (30 mL), dried over anhydrous Na2SO4 and concentrated. The obtained crude was purified by silica gel 10 (230-400 mesh) column chromatography using 10% Methanol in dichloromethane as eluent to afford the title compound 30 (3 g) as white solid. 'H NMR (400MHz, CDC13) 8 = 10.70 (s, 1H), 10.04 (s, 1H), 8.54 (d, J=2.0 Hz, 1H), 7.92 (dd, J=2.4, 8.8 Hz, 1H), 7.18 (d, J=8.8 Hz, 1H), 5.59 (br s, 2H), 4.43 (q, ./=7.0 Hz, 2H), 3.59 (t, ./=5.4 Hz, 2H), 3.15 - 2.89 (m, 6H), 2.72 - 2.51 (m, 6H), 1.72 - 1.58 (m, 5H), 1.28 (t, ./=7.3 Hz, 3H), 0.97 (t, ./=7.3 Hz, 3H), LCMS (M+H) =564.9, 15 purity~95.5%. EXAMPLE 54 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phcnyl)-5-ethyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,l-f][1,2,4]triazine-6-carbaldehyde (laa) A solution of 30 (300 mg, 0.53 mmol) in absolute ethanol (8 mL) was added IM aqueous KOH 20 solution (8 mL) and stirred at 95°C in a sealed tube for 72h. The reaction mixture was concentrated completely under reduced pressure. The crude was added water (8 mL) and stirred at RT for 10 min, the resulted solid was filtered, washed with diethyl ether (3X4 mL) and dried to afford the title laa (220 mg) as brown solid. ]H NMR (300MHz, CDC13) 8 = 10.19 (s, 1H), 9.72 (brs, 1H), 8.55 (d, J=2.4Hz, 1H), 7.89 (dd, J=2.4Hz, 8.8Hz; 1H), 7.18 (d, J=8.7Hz,lH), 4.40- 25 4.33 (m, 2H), 3.58 (brs, 2H), 3.31-3.20 (m, 4H), 3.09 (brm, 4H), 2.63-2.54 (m, 6H), 1.80-1.73 (m, 2H), 1.63 (t, J=7.2 Hz, 3H), 1.30 (t, J=1A Hz, 3H), 1.00 (t, J=12 Hz, 3H), LCMS (M+H) =544.5, purity~95%. CA 03001728 2018-04-11 WO 2017/085056 -83- PCT/EP2016/077720 EXAMPLE 55 5 (£)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7- propyl-3,4-dihydropyrrolo[2,l-f][1,2,4]triazine-6-carbaldehyde oxime (lab) To a stirring solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (laa) (100 mg, 0.183 mmol) in ethanol (3 mL) and water (0.5 mL) was added hydroxylamine hydrochloride (70 10 mg, 0.458 mmol) and the reaction mixture was heated to 85 °C for 4 h. On completion, the reaction mixture was concentrated under reduced pressure. The obtained residue was stirred in water (1 mL), filtered, washed with diethyl ether (2x3 mL) and dried to afford the title lab (24 mg) as white solid. rH NMR (400MHz, DMSO-d6) 8 = 11.64 (s, 1H), 10.95 (brs, 1H), 8.21(s, 1H), 7.90 (brs, 1H), 7.43 (br, 1H), 5.32 (br, 1H), 4.24-4.23 (m, 2H), 3.74-3.69 (m, 3H), 3.53-3.36 15 (m, 2H), 3.19 (brm, 3H), 3.09-3.03 (m, 2H), 2.96-2.66 (m, 5H), 1.63-1.57 (m, 2H), 1.36-1.33 (m, 3H), 1.14 (t, J=1A Hz, 3H), 0.88 (t, ./=7.4 Hz, 3H), LCMS (M+H) =561.3, purity~94%+2.7% (mixture of syn & anti isomers). NH2OCH3xHC1, EtOH H2O 20 CA 03001728 2018-04-11 WO 2017/085056 -84- PCT/EP2016/077720 EXAMPLE 56 2-(2-Ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,l-/] [l,2,4]triazine-6-carbaldehyde O-methyloxime (lac) 5 To a stirring solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (laa) (100 mg, 0.183 mmol) in ethanol (2 mL) and water (0.5 mL) was added methoxylamine hydrochloride (38.3 mg, 0.458 mmol) and the reaction mixture was heated to 85 °C for 4 h. On completion, the reaction mixture was concentrated; the obtained crude was dissolved in 10% methanol and 10 dichloromethane (10 mL) mixture and washed with water (3 mL). The organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure to afford the title lac (32 mg) as brown solid. ]H NMR (400MHz, CD3OD) 8 = 8.22 (s, 1H), 8.06 (d, J=2.4Hz, 1H), 7.93 (dd, J=2.4Hz, 8.8Hz; 1H), 7.37 (d, J=8.8Hz,lH), 4.30 (q, J=6.8Hz, 2H), 3.91 (s, 3H), 3.66-3.63 (m, 2H), 3.15-3.04 (m, 8H), 2.72-2.62 (m, 6H), 1.73-1.67 (m, 2H), 1.48 (t, J =7.4Hz, 3H), 1.21 (t, 15 J=7.2 Hz, 3H), 0.96 (t, J=1.2 Hz, 3H), LCMS (M+H) =575.3, purity-94.7%+4.7% (mixture of syn & anti isomers). SCHEME 27: 20 EXAMPLE 57 (£)-2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5-ethyl-4-oxo-7- propyl-3,4-dlhydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde O-(2-hydroxyethyl) oxime (lad) 25 To a stirring solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (laa) (80 mg, 0.146 mmol) in ethanol (3 mL) and water (0.5 mL) was added 2-Aminoxyethanol (39.6 mg, 0.51 CA 03001728 2018-04-11 WO 2017/085056 -85- PCT/EP2016/077720 mmol) and the reaction mixture was heated to 85°C for 16h. On completion, the reaction mixture was concentrated; the obtained crude was purified by PREP HPLC using 4% methanol in dichloromethane to afford the title lad (20 mg) as a brown solid. 'H NMR (400MHz, CD3OD) 5 = 8.30 (s, 1H), 8.05 (d, J=2.4Hz, 1H), 7.92 (dd, J=2.4Hz, 8.8Hz; 1H), 7.36 (d, J=8.8Hz,lH), 5 4.30 (q, ,7=7.6Hz, 2H), 4.20-4.17 (m, 2H), 3.84-3.82 (m, 2H), 3.62-3.60 (m, 2H), 3.15-3.04 (m, 6H), 2.62-2.61 (m, 4H), 2.53-2.50 (m, 2H), 1.73-1.68 (m, 2H), 1.48 (t, ,7=6.8Hz, 3H), 1.21 (t, .7=7.2 Hz, 3H), 0.96 (t, J=1A Hz, 3H), LCMS (M+H) =605.8, purity-93.6%. SCHEME 28: 10 EXAMPLE 58: 2-(5-((4-(2-chloroethyl)piperazin-l-yl)sulfonyl)-2-ethoxyphenyl)-5-methyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (31) 15 To a stirred solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (Iw) (200 mg, 0.376 mmol) in dichloromethane (5 mL), was added SOCI2 (0.054 mL, 0.753 mmol) at 0 °C. The reaction mixture was heated to 50 °C and stirred for 12 h. On completion, the reaction mixture was concentrated under reduced pressure. The obtained residue was diluted with water (10 mL) 20 and extracted with dichloromethane (2x15 mL). The combined organic layers were dried over anhydrous sodum sulfate and concentrated under reduced pressure to afford the title compound CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -86 - 31 (200 mg) as a brown solid, which was directly taken for next reaction without further purification. MS (M+H) = 550.2. EXAMPLE 59 2-(4-((4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l- 5 f][l>2,4]triazin-2-yl)phenyl)sulfonyl)piperazin-l -yl)ethyl nitrate (lae) To a stirred solution of 2-(5-((4-(2-chloroethyl)piperazin-l-yl)sulfonyl)-2-ethoxyphenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (31) (200 mg, 0.364 mmol) in acetonitrile (5 mL), was added AgNO? (247.5 mg, 1.457 mmol) at RT. The reaction was then heated to 50 °C and stirred for 16 h. On completion, the reaction mixture was 10 cooled to RT and filtered through a celite bed. The filtrate was concentrated under reduced pressure, diluted with water (15 mL) and extracted with ethyl acetate (2 X 20 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to afford the crude product (260 mg; LCMS~82%) as an pale yellow solid. A portion of the crude product (160 mg) was purified by reverse phase preparative HPLC using 15 0.1% formic acid in water and acetonitrile to afford the title compound lae (23 mg) as an off- white solid. NMR (400 MHz, CDC13) 3 = 10.19 (s, 1H), 9.69 (br s, 1H; D2O exchangeable), 8.52 (d, .7=2.4 Hz, 1H), 7.87 (dd, J=2.4, 8.8 Hz, 1H), 7.17 (d, ,7=8.8 Hz, 1H), 4.50 (t, ,7=5.4 Hz, 2H), 4.36 (q, J=1A Hz, 2H), 3.22 (t, .7=7.3 Hz, 2H), 3.09 -3.06 (m, 4H), 2.79 (s, 3H), 2.72 (t, ,7=5.4 Hz, 2H), 2.64 - 2.61 (m, 4H), 1.78 - 1.75 (m, 2H), 1.63 (t, .7=7.1 Hz, 3H), 0.99 (t, ,7=7.3 20 Hz, 3H). LCMS (M+H) = 577.7, purity-94.2%. EXAMPLE 60 (E)-2-(4-((4-ethoxy-3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,l-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin-1-yl)ethyl nitrate (laf) To a stirred solution of 2-(4-((4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4- 25 dihydropyrrolo[2,l-f][l,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin-l-yl)ethyl nitrate (lae) (100 mg, 0.173 mmol) in ethanol (2 mL) and water (0.5 mL), was added hydroxylamine hydrochloride (30.14 mg, 0.433 mmol) and the reaction mixture was heated to 85 °C for 8 h. On completion, the reaction mixture was concentrated under reduced pressure. The obtained residue was dissolved in water (5 mL) and extracted with ethyl acetate (2X15 mL). The combined 30 organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% formic acid CA 03001728 2018-04-11 WO 2017/085056 -87- PCT/EP2016/077720 in water and acetonitrile to afford the title compound lae (20 mg) as an off-white solid. 'H NMR (300 MHz, CD3OD) 5 = 8.23 (s, 1H), 8.05 (d, J=1.8 Hz, 1H), 7.91 (dd, J=1.8, 8.8 Hz, 1H), 7.37 (d, J=8.8 Hz, 1H), 4.57 (t, J=5.3 Hz, 2H), 4.30 (q, .7=6.9 Hz, 2H), 3.18 - 2.92 (m, 6H), 2.73 (t, .7=5.3 Hz, 2H), 2.64 - 2.60 (m, 7H), 1.73 - 1.70 (m, 2H), 1.48 (t, .7=6.9 Hz, 3H), 0.96 (t, J=1.5 5 Hz, 3H). LCMS (M+H) = 592.4, purity-92.3%+l.3% (mixture of syn & anti isomers). SCHEME 29: 10 EXAMPLE 61 2-(5-((4-(2-chloroethyl)piperazin-l-yl)sulfonyl)-2-ethoxyphenyl)-5-ethyl-4-oxo-7-propyl- 3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (32) To a stirred solution of 2-(2-ethoxy-5-((4-(2-hydroxyethyl)piperazin-l-yl)sulfonyl)phenyl)-5- ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (laa) (200 mg, 15 0.366 mmol) in dichloromethane (5 mL), was added SOCI2 (0.053 rnL, 0.733 mmol) at 0 °C. The reaction mixture was heated to 50 °C and stirred for 12 h. On completion, the reaction mixture was concentrated under reduced pressure. The obtained residue was diluted with water (10 mL) and extracted with dichloromethane (2x15 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure to afford the title compound 20 32 (200 mg) as a brown solid, which was directly taken for next reaction without further purification. LCMS (M+H) = 564.2, purity-86.6%. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -88 - EXAMPLE 62 2-(4-((4-ethoxy-3-(5-ethyl-6-formyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazin- 2-yl)phenyl)sulfonyl)piperazin-l-yl)ethyl nitrate (lag) To a stirred solution of 2-(5-((4-(2-chloroethyl)piperazin-l-yl)sulfonyl)-2-ethoxyphenyl)-5- 5 ethyl-4-oxo-7-propyl-3,4-dihydropyrrolo[2,l-f][l,2,4]triazine-6-carbaldehyde (32) (200 mg, 0.355 mmol) in acetonitrile (5 mL), was added AgNO3 (241 mg, 1.420 mmol) at RT. The reaction was then heated to 50 °C and stirred for 16 h. On completion, the reaction mixture was cooled to RT and filtered through a celite bed. The filtrate was concentrated under reduced pressure, diluted with water (15 mL) and extracted with ethyl acetate (2 X 20 mL). The 10 combined organic layers were dried over anhydrous sodium sulfate and concentrated under reduced pressure. The crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lag (30 mg) as a brown solid. NMR (400 MHz, CDC13) 8 = 10.19 (s, 1H), 9.74 (br s, 1H; D2O exchangeable), 8.53 (d, 7=2.2 Hz, 1H), 7.87 (dd, J=2.2, 8.8 Hz, 1H), 7.18 (d, 7=8.8 Hz, 1H), 4.51 (t, 7=5.3 Hz, 2H), 4.37 15 (q, J=1.2 Hz, 2H), 3.35 - 3.18 (m, 4H), 3.09 - 3.06 (m, 4H), 2.73 (t, 7=5.3 Hz, 2H), 2.63 - 2.61 (m, 4H), 1.78 - 1.75 (m, 2H), 1.63 (t, 7=7.2 Hz, 3H), 1.30 (t, 7=7.6 Hz, 3H), 1.00 (t, 7=7.3 Hz, 3H). LCMS (M+H) = 591.3, purity-96.5%. EXAMPLE 63 (jE)-2-(4-((4-ethoxy-3-(5-ethyl-6-((hydroxyimino)methyl)-4-oxo-7-propyl-3,4- 20 dihydropyrrolo[2,l-f][l,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin-l-yl)ethyl nitrate (lah) To a stirred solution of 2-(4-((4-ethoxy-3-(5-ethyl-6-formyl-4-oxo-7-propyl-3,4- dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)phenyl)sulfonyl)piperazin- -yl)ethyl1 nitrate (lag) (90 mg, 0.152 mmol) in ethanol (3 mL) and water (0.5 mL), was added hydroxylamine hydrochloride (26.4 mg, 0.380 mmol) and the reaction mixture was heated to 85 °C for 16 h. On 25 completion, the reaction mixture was concentrated under reduced pressure. The obtained residue was dissolved in water (5 mL) and extracted with ethyl acetate (2X15 mL). The combined organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lah (23 mg) as a brown solid. JH NMR 30 (300 MHz, CD3OD) 8 = 8.22 (s, 1H), 8.05 (d, J=2.2 Hz, 1H), 7.91 (dd, J=2.2, 8.8 Hz, 1H), 7.37 (d, 7=8.8 Hz, 1H), 4.57 (t, 7=5.3 Hz, 2H), 4.31 (q, 7=6.9 Hz, 2H), 3.20 - 2.92 (m, 8H), 2.73 (t, 7=5.3 Hz, 2H), 2.63 - 2.61 (m, 4H), 1.73 - 1.70 (m, 2H), 1.49 (t, 7=6.9 Hz, 3H), 1.22 (t, 7=7.3 CA 03001728 2018-04-11 WO 2017/085056 -89- PCT/EP2016/077720 Hz, 3H), 0.96 (t, .7=7.3 Hz, 3H). LCMS (M+H) = 606.3, purity-92.3%+3.4% (mixture of syn & anti isomers). SCHEME 30: CH3ONH2 HCL Eton, h2o 80°C, 16 hC 5 EXAMPLE 64 (E)-2-(4-(4-ethoxy-3-(6-((methoxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo [l,2-/][l,2,4]triazin-2-yl)phenylsulfonyl)piperazin-l-yl)ethyl nitrate (lai) To a stirred solution of 2-(4-(4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4- 10 dihydropyrrolo[l,2-/][l,2,4]triazin-2-yl)phenylsulfonyl)piperazin-l-yl)ethyl nitrate (lae) (90 mg, 0.164 mmol) in ethanol (5 mL) and water (0.5 mL), was added O-methoxylamine hydrochloride (34.4 mg, 0.412 mmol) and the reaction mixture was heated to 85 °C for 8 h. The reaction was monitored by LCMS. The reaction mixture was then cooled to room temperature and concentrated under reduced pressure. The obtained residue was dissolved in water (5 mL) and 15 extracted with ethyl acetate (3 X 25 mL). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure. The crude LCMS analysis showed 45% of desired oxime along with 18% of chloro substituted oxime. The obtained crude product was dissolved in acetonitrile (10 mL), added AgNOa (80 mg) and stirred 50 °C for 12 h. The reaction mixture was then cooled to room temperature and concentrated under reduced pressure. The 20 obtained residue was dissolved in water (5 mL) and extracted with ethyl acetate (3 X 25 mL). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure. The crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lai (18 mg) as an off-white solid, ’h NMR (400 MHz, CD3OD): 3 8.24 (s, 1H), 8.04 (d, J = 2.4 Hz, 1H), 7.91 (dd, J= 2.4, 8.8 Hz, 25 1H), 7.37 (d, J= 8.8 Hz, 1H), 4.56 (t, J= 5.1 Hz, 2H), 4.34 - 4.24 (m, 2H), 3.91 (s, 3H), 3.17 - 2.94 (m, 6H), 2.77 - 2.68 (m, 2H), 2.67 - 2.49 (m, 7H), 1.70 (dd, J= 7.3, 14.7 Hz, 2H), 1.48 (t, J = 7.1 Hz, 3H), 0.95 (t, J= 7.3 Hz, 3H); LCMS (ES): m/z 606.3 [M+H+]; purity-95. l%+4.2% (mixture of anti & syn isomers). CA 03001728 2018-04-11 WO 2017/085056 -90- PCT/EP2016/077720 SCHEME 31: EXAMPLE 65 (E)-2-(4-(4-ethoxy-3-(6-((2-hydroxyethoxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- 5 dihydropyrrolo[l,2-/][1,2,4]triazin-2-yl)phenylsulfonyl)piperazin-l-yl)ethyl nitrate (lak): To a stirred solution of 2-(4-(4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4- dihydropyrrolo[l,2-;/][l,2,4]triazin-2-yl)phenylsulfonyl)piperazin-l-yl)ethyl nitrate (lae) (105 mg, 0.182 mmol) in ethanol (5 mL) was added 2-(aminoxy)ethanol (35.13 mg, 0.455 mmol) at room temperature and stirred at 80 °C for 8 h. After completion of reaction (monitored by TLC), 10 the reaction mixture was concentrated under reduced pressure. The obtained residue was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lak (28 mg) as a white solid. 1H NMR (400 MHz, CD3OD): J 8.28 (s, 1H), 8.03 (d, J = 2.4 Hz, 1H), 7.90 (dd, J = 2.4, 8.8 Hz, 1H), 7.37 (d, J = 8.8 Hz, 1H), 4.57 (t, J = 5.1 Hz, 2H), 4.29 (q, J= 7.0 Hz, 2H), 4.20 - 4.17 (m, 2H), 3.83 -3.81 (m, 2H), 3.03 -3.01 (m, 6H), 2.74 15 - 2.71 (m, 2H), 2.61 - 2.59 (m, 6H), 1.72 - 1.68 (m, 2H), 1.43 (t, J = 7.0 Hz, 3H), 0.95 (t, J = 12 Hz, 3H); LCMS (ES): m/z 636.3 [M+H+]; purity~94.4%+2.2% (mixture of anti & syn isomers). SCHEME 32: 20 EXAMPLE 66 tert-butyl 4-(2-(benzoyloxy)ethyl)piperidine-l-carboxylate (34) To a stirred solution of tert-butyl 4-(2-hydroxyethyl)piperidine-l-carboxylate (33) (8.5 g, 37.06 mmol) in THF (160 mL), was added triethylamine (10.2 mL, 74.12 mmol) at room 25 temeperature. After 5 min, benzoyl chloride (4.73 mL, 40.77 mmol) was added dropwise at CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -91 - 0 °C under inert atmoshphere. After addition, the resultant reaction mixture was stirred at room temperature for 2 h. After completion of reaction (monitored by TLC), the reaction was quenched with water (75 mL) and extracted with ethyl acetate (2 X 100 mb). The combined organic layer was dried over anhydrous sodium sulphate and concentrated 5 under reduced pressure to afford 34 (10 g) as a brown liquid, which was directly taken for next reaction without further purification. 1H NMR (300 MHz, CDCh): 6 8.04 (d,]=7.3 Hz, 2H), 7.58 - 7.50 (m, 1H), 7.49 - 7.41 (m, 2H), 4.38 (tj= 6.6 Hz, 2H), 4.12 - 4.08 (m, 2H), 2.72 - 2.68 (m, 2H), 1.81- 1.68 (m, 5H), 1.46 (s, 9H), 1.28 - 1.10 (m, 2H). 10 SCHEME 33: EXAMPLE 67 2-(piperidin-4-yl)ethyl benzoate. TFA salt (35) To a stirred solution of compound 34 (10 g) in CH2CI2 (100 mL), was added trifluoroacetic 15 acid (6.89 mL, 90.09 mmol) dropwise at 0 °C under inert atmoshphere. After addition, the resultant reaction mixture was stirred at room temperature for 2 h. After completion of reaction (monitored by TLC), the reaction solution was concentrated under reduced pressre. The residue was co-distilled with CH2CI2 (3 X 50 mL). The solid obtained was triturated with diethyl ether (50 mL), filtered and dried under vacuum to afford title 35 (4 20 g, 33% in two steps) as a brown solid. ’H NMR (400 MHz, DMSO-d6): 6 8.93 (br s, 1H; D2O exchangeable), 8.85 (br s, 1H; D20 exchangeable), 8.01 - 7.92 (m, 2H), 7.72 - 7.62 (m, 1H), 7.59 - 7.48 (m, 2H), 4.34 (tj= 6.2 Hz, 2H), 3.23 -3 .21 (m, 2H), 2.86 - 2.81 (m, 2H), 1.87 (br d,/ = 13.9 Hz, 2H), 1.81 - 1.63 (m, 3H), 1.43 - 1.26 (m, 2H); LCMS (ES): m/z 234.1 [M+H+]; purity~99%. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -92 - EXAMPLE 68 5-(4-(2-(benzoyloxy)ethyl)piperidin-l-ylsulfonyl)-2-ethoxybenzoic acid (36) To a stirred solution of 35 (4.07 g, 17.49 mmol) in CH2CI2 (40 mL) was added triethylamine (11.10 mL, 79.54 mmol) at room temperature. After addition, a solution of 9 (4.2 g, 15.90 5 mmol) in CH2CI2 (20 mL) was added dropwise at 0 °C under inert atmosphere. The resultant reaction mixture was stirred at room temperature for 12 h. After completion of reaction (monitored by TLC), the reaction mixture was concentrated and diluted with saturated NaHCOs solution (50 mL). The resultant solution was then neutralized with saturated citric acid solution (50 mL) and extracted with ethyl acetate (3 x 200 mL). The 10 combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford Compound 36 (5.78 g, 78%) as a brown solid. 1H NMR (300 MHz, DMSO-d6): 6 13.02 (br s, 1H; D2O exchangeable), 7.97 - 7.88 (m, 3H), 7.81 (dd,/ = 2.4, 8.8 Hz, 1H), 7.68 - 7.60 (m, 1H), 7.55 - 7.46 (m, 2H), 7.33 (dj= 8.8 Hz, 1H), 4.27 (t,/ = 6.4 Hz, 2H), 4.20 (q,/ = 7.0 Hz, 2H), 3.61 (br d,/= 11.7 Hz, 2H), 2.20 (brt,J= 10.8 Hz, 2H), 15 1.79 (br d, / = 11.7 Hz, 2H), 1.63 (q,/ = 6.4 Hz, 2H), 1.46 -1 41 (m, 1H), 1.35 (t,/ = 7.0 Hz, 3H), 1.30 - 1.17 (m, 2H); LCMS (ES): m/z 462.2 [M+H+]; purity~95%. CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 SCHEME 34: EXAMPLE 69 5 ethyl l-(5-(4-(2-(benzoyloxy)ethyl)piperidin-l-ylslfonyl)-2-ethoxybenzamido)-4- formyl-3-methyl-5-propyl-lff-pyrrole-2-carboxylate (37) To a stirried solution of 5-(4-(2-(benzoyloxy)ethyl)piperidin-l-ylsulfonyl)-2- ethoxybenzoic acid (36) (4.2 g, 9.11 mmol) in DMF (30 ml), was added TBTU (5.85 g, 18.22 mmol) and diisopropylethyl amine (4.7 mL, 27.33 mmol) at room temperature and 10 stirred for 20 min. To this, ethyl l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2- carboxylate (27) (2.168 g, 9.11 mmol) was added and stirred the reaction mixture at room temperature for 16 h. After completion of reaction (monitored by TLC), the reaction was quenched with ice-cold water (100 mL) and extracted with ethyl acetate (3 x 300 mL). The combined organic layer was dried over anhydrous sodium sulphate and concentrated 15 under reduced pressure. The crude product was purified by silica gel column chromatography using 10-50 % ethyl acetate gradient in petroleum ether to afford the title CA 03001728 2018-04-11 _ _ WO 2017/085056 94 PCT/EP2016/077720 compound 37 (3.0 g, 48%) as a white solid. ’H NMR (300 MHz, CDCI3): 5 10.38 (s, 1H), 10.07 (s, 1H), 8.56 (d, J = 2.2 Hz, 1H), 7.99 (d,/ =7.3 Hz, 2H), 7.93 (dd, J = 2.4, 8.6 Hz, 1H), 7.59 - 7.50 (m, 1H), 7.48 - 7.36 (m, 2H), 7.17 (d,/ = 8.8 Hz, 1H), 4.41 (q,/ = 7.0 Hz, 2H), 4.33 (t,/ = 6.4 Hz, 2H), 4.28 - 4.13 (m, 2H), 3.81 (br d,/ = 10.8 Hz, 2H), 2.61 (s, 3H), 2.30 (br t,/ = 5 10.8 Hz, 2H), 1.84 - 1.81 (m, 2H), 1.76 - 1.52 (m, 9H), 1.47 - 1.33 (m, 3H), 1.27 (t,/ = 7.0 Hz, 3H), 0.95 (t,/= 7.3 Hz, 3H); LCMS (ES): m/z 682.3 [M+H+]; purity~98%. EXAMPLE 70 l-(2-ethoxy-5-(4-(2-hydroxyethyl)piperidm-l-ylsulfonyl)benzamido)-4-formyl-3- methyl-5-propyl-l/f-pyrrole-2-carboxylic acid (38) 10 To a stirred solution of 37 (3.1 g, 4.55 mmol) in methanol (15 mL) and water (15 mL), was added NaOH (3.1 g, w/w) at room temperature. The reaction mixture was heated to 65 °C and stirred for 16 h. After completion of reaction (monitored by TLC), methanol was concentrated under reduced pressure. The obtained aqueous reaction solution was neutralized (pH~7) with aqueous IN HC1 solution (10 mL). The solid precipitated was 15 filtered and dried under vacuum to afford 38 (2.6 g, 87%) as an off-white solid. 1H NMR (300 MHz, DMSO-d6): 8 12.89 (s, 1H; D2O exchangeable), 11.45 (br s, 1H; D2O exchangeable), 7.90 (d,/ = 2.2 Hz, 1H), 7.83 (dd,/ = 2.2, 8.8 Hz, 1H), 7.39 (d,/ = 8.8 Hz, 1H), 4.42 (br s, 1H; D20 exchangeable), 4.26 (q,/ = 7.0 Hz, 2H), 3.60 (br d,/ = 11.4 Hz, 2H), 3.43 - 3.37 (m, 2H), 2.86 - 2.83 (m, 2H), 2.54 (s, 3H), 2.19 (br t,/ = 10.8 Hz, 2H), 1.71 (br dj= 11.7 20 Hz, 2H), 1.64 - 1.51 (m, 2H), 1.40 (t,J = 7.0 Hz, 3H), 1.36 - 1.26 (m, 3H), 1.25 - 1.03 (m, 3H), 0.90 (tj= 7.3 Hz, 3H); LCMS (ES): m/z 550.3 [M+H+]; purity~94%. EXAMPLE 71 l-(2-ethoxy-5-(4-(2-hydroxyethyl)piperidin-l-ylsulfonyl)benzamido)-4-formyl-3- methyl-5-propyl-IH-pyrrole-2-carboxamide (39) 25 To a stirred solution of 38 (2.4 g, 4.37 mmol) in DMF (25 mL), TBTU (2.80 g, 8.74 mmol) and diisopropylethylamine (2.3 mL, 13.11 mmol) were added and stirred at room temperature for 20 minutes. To this, ammonium chloride (468 mg, 8.74 mmol) was added and the reaction mixture was stirred at room temperature for 16 h. After completion of reaction (monitored by TLC), the reaction was quenched with ice-cold water (75 mL) and 30 extracted with 5% methanol in CH2C12 (2 x 100 mL). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The obtained CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -95 - crude product was purified by silica gel column chromatography using 2-8% gradient methanol in dichloromethane as eluent to afford the title compound 39 (2 g, 83%) as a pale yellow solid. 1H NMR (300 MHz, DMSO-de): 5 11.36 (s, 1H; D2O exchangeable), 9.96 (s, 1H), 7.88 (d,J = 2.6 Hz, 1H), 7.86 - 7.81 (m, 1H), 7.48 (br s, 1H; D2O exchangeable), 7.39 (br 5 d,/ = 8.8 Hz, 1H), 7.31 (br s, 1H; D2O exchangeable), 4.35 - 4.19 (m, 2H+OH), 3.67 - 3.53 (m, 2H), 3.43 - 3.34 (m, 2H), 2.93 - 2.72 (m, 2H), 2.41 (s, 3H), 2.31 - 2.13 (m, 2H), 1.72 (br dj= 11.7 Hz, 2H), 1.62 - 1.50 (m, 2H), 1.40 (tj= 7.0 Hz, 3H), 1.36 - 1.21 (m, 5H), 0.89 (t,/ = 7.2 Hz, 3H); LCMS (ES): m/z 549.3 [M+H+]; purity~91%. EXAMPLE 72 10 2-(2-ethoxy-5-(4-(2-hydroxyethyl)piperidin-l-ylsulfonyl)phenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[l,2-/][l,2,4]triazine-6-carbaldehyde (40) To a stirred solution of 39 (400 mg, 0.72 mmol) in absolute ethanol (6 mL) was added IM aqueous KOH solution (5 mL) and stirred at 100 °C in a sealed tube for 96 h. The reaction mixture was concentrated completely under reduced pressure. The obtained residue was 15 diluted with water (5 mL) and stirred at room temperature for 10 min. The solid precipitated was filtered and dried to afford the title compound 40 (280 mg, 72%) as a brown solid. 1H NMR (400 MHz, CDCI3): 6 10.19 (s, 1H), 9.68 (br s, 1H; D2O exchageable), 8.53 (d,/= 2.0 Hz, 1H), 7.89 (dd,/= 2.4, 8.8 Hz, 1H), 7.16 (d,/ = 8.8 Hz, 1H), 4.36 (q,/ = 6.8 Hz, 2H), 3.81 (br dj= 11.7 Hz, 2H), 3.68 - 3.64 (m, 2H), 3.22 (t,J = 7.3 Hz, 2H), 2.79 (s, 3H), 20 2.40 - 2.27 (m, 2H), 1.81 - 1.72 (m, 4H), 1.62 (t,/ = 6.8 Hz, 3H), 1.52 - 1.47 (m, 3H), 1.42 - 1.33 (m, 3H), 0.99 (t,/ = 7.3 Hz, 3H); LCMS (ES): m/z 531.3 [M+H+]; purity~92%. EXAMPLE 73 (E)-2- (2-ethoxy-5- (4-(2-hydroxyethyl)piperidin-1-ylsulfonyl)phenyl)- 5-methyl-4- oxo-7-propyl-3,4-dihydropyrrolo[l,2-/|[l,2,4]triazine-6-carbaldehyde oxime (lai) 25 To a stirred solution of compound 40 (140 mg, 0.264 mmol) in ethanol (3 mL) and water (0.5 mL), was added hydroxylamine hydrochloride (45.88 mg, 0.66 mmol) and the reaction mixture was heated to 90 °C for 16 h. After completion of reaction (monitored by TLC), the reaction mixture was concentrated under reduced pressure. The obtained residue was dissolved in water (5 mL) and extracted with ethyl acetate (3 X 25 mL). The combined 30 organic layer was dried over anhydrous Na2SCU and concentrated under reduced pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% CA 03001728 2018-04-11 WO 2017/085056 -96- PCT/EP2016/077720 formic acid in water and acetonitrile to afford the title compound lai (80 mg, 55%) as an off-white solid. 1H NMR (300 MHz, DMSO-ds) 8 11.55 (br s, 1H; D2O exchageable), 10.95 (s, 1H; D2O exchageable), 8.21 (s, 1H), 7.92 - 7.79 (m, 2H), 7.37 (d,/= 8.3 Hz, 1H), 4.31 (t,/ = 5.1 Hz, 1H; D2O exchageable), 4.21 (qj= 6.8 Hz, 2H), 3.60 (br dj= 11.2 Hz, 2H), 3.39 (q, J = 5 5.9 Hz, 2H), 2.94 (br t,/ = 7.6 Hz, 2H), 2.55 (s, 3H), 2.24 (br t,/ = 11.0 Hz, 2H), 1.76 - 1.52 (m, 4H), 1.39 - 1.26 (m, 6H), 1.24 - 1.09 (m, 2H), 0.88 (t,/ = 7.3 Hz, 3H); LCMS (ES): m/z 546.3 [M+H+]; purity~95.5%. SCHEME 35: 10 EXAMPLE 74 2-(5-(4-(2-chloroethyl)piperidin-l-ylsulfonyl)-2-ethoxyphenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[l,2-/|[l,2,4]triazine-6-carbaldehyde (41) To a stirred solution of 2-(2-ethoxy-5-(4-(2-hydroxyethyl)piperidin-l-ylsulfonyl)phenyl)- 15 5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[l,2-/][l,2,4]triazine-6-carbaldehyde (40) (100 mg, 0.188 mmol) in dichloromethane (5 mL), was added SOCI2 (0.06 mL, 0.943 mmol) at room temperature. The reaction mixture was heated to 50 °C and stirred for 72 h. TLC showed product formation along with unreacted 40. The reaction mixture was cooled to room temperature, added SOCI2 (0.12 mL, 1.88 mmol) and the reaction mixture was stirred 20 at 80 °C for additional 48 h. The reaction mixture was then cooled to room temperature and concentrated under reduced pressure. The obtained residue was diluted with water (10 mL) and extracted with dichloromethane (2 x 20 mL). The combined organic layer was CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 -97 - dried over anhydrous sodum sulphate and concentrated under reduced pressure to afford the crude compound 41. The reaction was repeated on 150 mg scale and both the crude materials were combined and purified by reverse phase purification by Grace instrument to afford the title 5 compound 41 (120 mg) as an off-white solid. m NMR (300 MHz, CDCI3): 6 10.19 (s, 1H), 9.71 (s, 1H; D20 exchageable), 8.53 (dj= 2.2 Hz, 1H), 7.89 (dd,/ = 2.2, 8.8 Hz, 1H), 7.16 (d,] = 8.8 Hz, 1H), 4.36 (q,/ = 7.0 Hz, 2H), 3.84 (br d,/ = 11.7 Hz, 2H), 3.54 (t,/ = 6.4 Hz, 2H), 3.22 (br t,/ = 7.3 Hz, 2H), 2.79 (s, 3H), 2.33 (br t,/ = 11.4 Hz, 2H), 1.86 - 1.66 (m, 6H), 1.62 (t,/ = 7.0 Hz, 3H), 1.45 - 1.26 (m, 3H), 1.00 (t,/ = 7.3 Hz, 3H); LCMS (ES): m/z 549.3 [M+H+]; 10 purity~90%. EXAMPLE 75 2-(l-(4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[l,2- f] [l,2,4]triazin-2-yl)phenylsulfonyl)piperidin-4-yl)ethyl nitrate (42) To a stirred solution of 41 (140 mg, 0.255 mmol) in acetonitrile (5 mL), was added AgNOs 15 (173.5 mg, 1.02 mmol) at room temnperature. The reaction was then heated to 60 °C and stirred for 48 h. TLC showed product formation along with unreacted 41. The reaction mixture was cooled to room temperature, added AgNOs (260 mg, 1.53 mmol) and the reaction mixture was stirred at 60 °C for additional 48 h. The reaction mixture was then cooled to room temeparature and filtered though a celite bed. The filterate was 20 concentrated under reduced pressure, diluted with water (15 mL) and extracted with ethyl acetate (2 X 40 mL). The combined organic layers were dried over anhydrous sodum sulphate and concentrated under reduced pressure. The crude product was purified by silica gel purification by Grace instrument (20-50% gradient ethylacetate in petroleum ether as an eluent) to afford the title compound 42 (56 mg) as a pale yellow solid. *H NMR 25 (400 MHz, CDCh): 6 10.19 (s, 1H), 9.73 (br s, 1H; D2O exchageable), 8.52 (d,/ = 2.2 Hz, 1H), 7.88 (dd, / = 2.2, 8.8 Hz, 1H), 7.17 (d,/ = 8.8 Hz, 1H), 4.46 (t,/ = 6.2 Hz, 2H), 4.36 (q,/ = 7.0 Hz, 2H), 3.84 (br d,/ = 11.0 Hz, 2H), 3.22 (br tj= 7.3 Hz, 2H), 2.79 (s, 3H), 2.44 - 2.23 (m, 2H), 1.84 - 1.66 (m, 6H), 1.62 (t, / = 7.0 Hz, 3H), 1.44 - 1.41 (m, 3H), 0.99 (t,/ = 7.3 Hz, 3H); LCMS (M+H) = m/z 576.3 [M+H+]; purity~91.4%. CA 03001728 2018-04-11 WO 2017/085056 -98- PCT/EP2016/077720 EXAMPLE 76 (E)-2-(l-(4-ethoxy-3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- dihydro pyrrolo[l,2-/J [l,2,4]triazin-2-yl)phenylsulfonyl)piperidin-4-yl)ethyl nitrate (lam) 5 To a stirred solution of 42 (100 mg, 0.173 mmol) in ethanol (3 mL) and water (0.5 mL), was added hydroxylamine hydrochloride (30.2 mg, 0.434 mmol) and the reaction mixture was heated to 90 °C for 16 h. After completion of reaction (monitored by TLC), the reaction mixture was concentrated under reduced pressure. The obtained residue was dissolved in water (5 mL) and extracted with ethyl acetate (2 X 15 mL). The combined organic layer 10 was dried over anhydrous Na2SO4 and concentrated under reduced pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lam (56 mg) as a brown solid. NMR (300 MHz, DMSO-dg): 6 11.54 (s, 1H; D2O exchageable), 10.95 (s, 1H; D2O exchageable), 8.21 (s, 1H), 7.95 - 7.79 (m, 2H), 7.37 (d,/ = 8.8 Hz, 1H), 4.52 (br t,/ = 6.6 Hz, 2H), 4.21 (q,/ 15 = 6.8 Hz, 2H), 3.63 (br d,/ = 12.2 Hz, 2H), 2.94 (br tj= 7.3 Hz, 2H), 2.55 (s, 3H), 2.25 (br t,/ = 11.0 Hz, 2H), 1.74 (br d,/ = 10.8 Hz, 2H), 1.67 - 1.53 (m, 4H), 1.35 - 1.30 (m, 4H), 1.27 - 1.13 (m, 2H), 0.87 (t,/ = 7.3 Hz, 3H); LCMS (M+H) = m/z 591.3 [M+H+]; purity~97.3%. SCHEME 36: 20 EXAMPLE 77 tert-butyl 4-(3-(benzoyloxy)propyl)piperazine-l-carboxylate (43) To a stirred solution of tert-butyl 4-(3-hydroxypropyl)piperazine-l-carboxylate (7.0 g, 28.64 mmol) in THF (50 mL), was added triethylamine (8 mL, 57.29 mmol) at room 25 temeperature. After 5 min, benzoyl chloride (3.66 mL, 31.51 mmol) was added dropwise at 0 °C under inert atmoshphere. After addition, the resultant reaction mixture was stirred at room temperature for 1h. After completion of reaction (monitored by TLC), the reaction was quenched with water (100 mL) and extracted with ethyl acetate (3 X 250 mL). The combined organic layer was dried over anhydrous sodium sulphate and concentrated CA 03001728 2018-04-11 WO 2017/085056 -99- PCT/EP2016/077720 under reduced pressure to afford 43 (7.8 g) as a off-white solid. 1H NMR (400 MHz, CDCh): 8 8.06 - 8.00 (m, 2H), 7.59 - 7.52 (m, 1H), 7.48 - 7.40 (m, 2H), 4.38 (t,J = 6.6 Hz, 2H), 3.50 - 3.39 (m, 4H), 2.53 (t, / = 7.3 Hz, 2H), 2.43 (br t, J = 4.9 Hz, 4H), 1.98 (quin, / = 6.8 Hz, 2H), 1.46 (s, 9H); LCMS (M+H) = m/z 349.7 [M+H+]; purity~83%. 5 EXAMPLE 78 3-(piperazin-l-yl)propyl benzoate TFA salt (44) To a stirred solution of compound 43 (7.8 g) in CH2CI2 (80 mL), was added trifluoroacetic acid (10.3 mL, 134.48 mmol) dropwise at 0 °C under inert atmoshphere. After addition, the resultant reaction mixture was stirred at room temperature for 8 h. After completion of 10 reaction (monitored by TLC), the reaction solution was concentrated under reduced pressre. The residue was co-distilled with CH2CI2 (3 X 100 mL). The solid obtained was triturated with diethyl ether (50 mL), filtered and dried under vacuum to afford title 44 (8.6 g) as a whilte solid. ‘H NMR (300 MHz, DMSO-d6): 8 9.13 (br s, 2H), 8.07 - 7.95 (m, 2H), 7.73 - 7.64 (m, 1H), 7.60 - 7.47 (m, 2H), 4.34 (t, / = 6.2 Hz, 2H), 3.40 - 2.94 (m, 10H), 15 2.16 - 1.96 (m, 2H); LCMS (M+H) = m/z 249.2 [M+H+]; purity~98%. SCHEME 37: 45 EXAMPLE 79 5-(4-(3-(benzoyloxy)propyl)piperazin-l-ylsulfonyl)-2-ethoxybenzoic acid (45) 20 To a stirred solution of Compound 44 (8.3 g, 31.43 mmol) in CH2CI2 (60 mL) was added triethylamine (26.5 mL, 188.63 mmol) at room temperature. After addition, a solution of 9 (8.57 g, 34.58 mmol) in CH2CI2 (40 mL) was added dropwise at 0 °Cunder inert atmosphere. The resultant reaction mixture was stirred at room temperature for 16 h. After completion of reaction (monitored by TLC), the reaction mixture was concentrated 25 and diluted with saturated NaHCOs solution (50 mL). The resultant solution was washed with ethyl acetate (100 mL). The aqueous solution was then neutralized with saturated CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 100- citric acid solution (100 ml) and extracted with ethyl acetate (3 x 300 mL). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the title 45 (9.1 g, 60%) as a white solid, NMR (400 MHz, CDCI3): 6 12. 17 (br s, 1H), 8.35 (d,/ = 2.4 Hz, 1H), 7.99 (d,/ = 7.3 Hz, 2H), 7.86 (dd,/ = 2.4, 8.8 Hz, 5 1H), 7.61 - 7.51 (m, 1H), 7.47 - 7.36 (m, 2H), 7.12 (d,/ = 8.8 Hz, 1H), 4.43 - 4.26 (m, 4H), 3.21 (br s, 4H), 2.92 (br s, 4H), 2.86 - 2.78 (m, 2H), 2.15 - 2.05 (m, 2H), 1.55 (t,/ = 6.8 Hz, 3H); LCMS (ES): m/z 477.2 [M+H+]; purity-97%. SCHEME 38: 45 10 EXAMPLE 80 ethyl l-(5-(4-(3-(benzoyloxy)propyl)piperazin-l-ylsulfonyl)-2-ethoxybenzamido)-4- formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (46) To a stirried solution of 5-(4-(3-(benzoyloxy)propyl)piperazin-l-ylsulfonyl)-2- ethoxybenzoic acid (45) (5.0 g, 10.50 mmol) in DMF (30 mL), was added TBTU (6.74 g, 21.0 15 mmol) and diisopropylethyl amine (5.5 mL, 31.5 mmol) at room temperature and stirred for 20 min. To this, ethyl l-amino-4-formyl-3-methyl-5-propyl-lH-pyrrole-2-carboxylate (14) (2.50 g, 10.5 mmol) was added and stirred the reaction mixture at room temperature for 16 h. After completion of reaction (monitored by TLC), the reaction was quenched with CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 101 - ice-cold water (100 mL) and extracted with ethyl acetate (3 x 250 ml). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using 30-50 % ethyl acetate gradient in petroleum ether to afford the title compound 46 (4.8 g, 65%) as 5 a brown liquid. *11 NMR (300 MHz, CDC13): 6 10.43 (s, 1H; D2O exchageable), 10.07 (s, 1H), 8.57 (s, 1H), 7.99 (br d,J = 7.7 Hz, 2H), 7.92 (br d,J = 8.8 Hz, 1H), 7.64 - 7.50 (m, 1H), 7.49 - 7.36 (m, 2H), 7.19 (d,/ = 8.8 Hz, 1H), 4.43 (qj= 6.6 Hz, 2H), 4.31 (br t,/ = 6.4 Hz, 2H), 4.23 (br d,J= 5.5 Hz, 2H), 3.04 (br s, 4H), 2.61 (s, 3H), 2.58 - 2.43 (m, 6H), 1.89 (td,/ = 6.6, 13.2 Hz, 2H), 1.73 - 1.57 (m, 5H), 1.33 - 1.20 (m, 5H), 0.96 (t,/ = 7.3 Hz, 3H); LCMS (ES): m/z 10 697.9 [M+H+]; purity~89%. EXAMPLE 81 l-(2-ethoxy-5-(4-(3-hydroxypropyl)piperazin-l-ylsulfonyl)benzamido)-4-formyl-3- methyl-5-propyl-l/f-pyrrole-2-carboxylic acid (47) To a stirred solution of 46 (2.8 g, 4.021 mmol) in methanol (30 mL) and water (30 mL), 15 was added NaOH (2.8 g, w/w) at room temperature. The reaction mixture was heated to 65 °C and stirred for 12 h. After completion of reaction (monitored by TLC), methanol was concentrated under reduced pressure. The obtained aqueous reaction solution was neutralized (pH~7) using aqueous IN HC1 solution (15 mL). The solid precipitated was filtered and dried under vacuum to afford 47 (1.875 g, 82%) as an off-white solid. 1H NMR 20 (300 MHz, DMSO-d6): 6 11.63 (br s, 1H), 9.98 (s, 1H), 7.90 (d,/ = 2.2 Hz, 1H), 7.83 (dd,/ = 2.2, 8.8 Hz, 1H), 7.40 (d, J = 8.8 Hz, 1H), 4.27 (q, J = 6.8 Hz, 2H), 3.36 (br t, J = 6.2 Hz, 2H+0H), 2.88 (br s, 6H), 2.54 (s, 3H), 2.43 (br s, 4H), 2.31 (br t,/ =7.2 Hz, 2H), 1.65 - 1.46 (m, 4H), 1.40 (t, ]= 7.0 Hz, 3H), 0.90 (t, / = 7.3 Hz, 3H); LCMS (ES): m/z 565.8 [M+H+]; purity~96%. 25 EXAMPLE 82 l-(2-ethoxy-5-(4-(3-hydroxypropyl)piperazin-l-ylsulfonyl)benzamido)-4-formyl-3- methyl-5-propyl-lW-pyrrole-2-carboxamide (48) To a stirred solution of 47 (1.875 g, 3.32 mmol) in DMF (15 mL), TBTU (2.135 g, 6.64 mmol) and diisopropylethylamine (1.7 mL, 9.97 mmol) were added and stirred at room 30 temperature for 20 minutes. To this, ammonium chloride (356 mg, 6.64 mmol) was added and the reaction mixture was stirred at room temperature for 16 h. After completion of CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 102 - reaction (monitored by TLC), the reaction was quenched with water (50 mL) and extracted with ethyl acetate (3 x 75 mL). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The obtained crude product was purified by silica gel column chromatography using 4-8% gradient methanol in 5 dichloromethane as an eluent to afford the title compound 48 (1.4 g, 74%) as a brown solid. iH NMR (400 MHz, CDC13): 8 10.61 (s, 1H; D2O exchageable), 10.05 (s, IH), 8.51 (d, ] = 2.4 Hz, IH), 7.89 (dd, J = 2.4, 8.8 Hz, IH), 7.17 (d, J = 8.8 Hz, IH), 5.66 (br s, 2H; D2O exchageable), 4.42 (qj= 7.2 Hz, 2H), 3.73 (t,/ = 5.1 Hz, 2H), 3.06 - 3.02 (m, 4H), 2.64 - 2.60 (m, 6H), 2.57 (s, 3H), 1.74 - 1.61 (m, 7H), 0.97 (t,/ = 7.3 Hz, 3H); LCMS (ES): m/z 564.3 10 [M+H+]; purity~95%. EXAMPLE 83 2-(2-ethoxy-5-(4-(3-hydroxypropyl)piperazin-l-ylsulfonyl)phenyl)-5-methyl-4-oxo- 7-propyl-3,4-dihydropyrrolo[l,2-/][l,2,4]triazine-6-carbaldehyde (49) To a stirred solution of 48 (500 mg, 0.88 mmol) in absolute ethanol (8 mL) was added IM 15 aqueous KOH solution (8 mL) and stirred at 100 °C in a sealed tube for 96 h. The reaction mixture was concentrated completely under reduced pressure. The obtained residue was diluted with water (10 mL); the solid precipitated was filtered and dried to afford the title compound 49 (350 mg, 72%) as a pale yellow solid. 1H NMR (300 MHz, CDC13): 8 10.18 (s, IH), 8.47 (br s, 1H), 7.83 (d,/ = 8.8 Hz, 1H), 7.15 (dj= 8.8 Hz, 1H), 4.37 -4.33 (m, 3H), 3.73 20 - 3.70 (m, 2H), 3.21 (br t,/ = 7.2 Hz, 2H), 3.07 - 3.03 (m, 4H), 2.78 (s, 3H), 2.63 - 2.58 (m, 6H), 1.89 - 1.50 (m, 7H), 0.99 (t, / = 7.3 Hz, 3H); LCMS (ES): m/z 546.7 [M+H+]; purity~91%. EXAMPLE 84 (E)-2-(2-ethoxy-5-(4-(3-hydroxypropyl)piperazin-l-ylsulfonyl)phenyl)-5-methyl-4- 25 oxo-7-propyl-3,4-dihydropyrrolo[l,2-/|[l,2,4]triazine-6-carbaldehyde oxime (lan) To a stirred solution of 49 (150 mg, 0.275 mmol) in ethanol (5 mL) and water (0.5 mL), was added hydroxylamine hydrochloride (47.8 mg, 0.68 mmol) and the reaction mixture was heated to 90 °C for 16 h. After completion of reaction (monitored by TLC), the reaction mixture was concentrated under reduced pressure. The obtained residue was dissolved in 30 water (5 mL) and extracted 10% methanol in dichloromethane solution (3X25 mL). The combined organic layer was dried over anhydrous Na2SO4 and concentrated under reduced CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 103 — pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lan (62 mg, 40%) as an off-white solid. *H NMR (400 MHz, DMSO-d6): S 11.56 (br s, 1H; D2O exchageable), 10.95 (br s, 1H; D20 exchageable), 8.21 (s, 1H), 7.91 - 7.80 (m, 2H), 7.39 (d, ] 5 = 8.8 Hz, 1H), 4.34 (br s, 1H; D2O exchageable), 4.22 (q,/ = 7.0 Hz, 2H), 3.38 - 3.34 (m, 2H), 3.00 - 2.81 (m, 6H), 2.55 (s, 3H), 2.43 - 2.40 (m, 4H), 2.35 - 2.27 (m, 2H), 1.62 - 1.58 (m, 2H), 1.54 - 1.45 (m, 2H), 1.34 (t,/ = 7.0 Hz, 3H), 0.87 (t, J = 7.3 Hz, 3H); LCMS (ES): m/z 561.4 [M+H+]; purity~95%. SCHEME 39: nh2oh,Etou 10 EXAMPLE 85 2-(5-(4-(3-chIoropropyl)piperazin-l-ylsulfonyl)-2-ethoxyphenyl)-5-methyl-4-oxo-7- propyl-3,4-dihydropyrrolo[l,2-/][l,2,4]triazine-6-carbaldehyde (50) 15 To a stirred solution of 2-(2-ethoxy-5-(4-(3-hydroxypropyl)piperazin-l- ylsulfonyl)phenyl)-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[l,2-/][l,2,4]triazine-6- carbaldehyde (49) (200 mg, 0.366 mmol) in dichloromethane (10 ml), was added SOC12 (0.1 ml, 1.83 mmol) at room temperature. The reaction mixture was heated to 70 °C and stirred for 12 h. After completion of reaction (monitored by TLC), the reaction mixture was CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 104 - cooled to room temperature and concentrated under reduced pressure. The obtained residue was diluted with water (10 mL) and extracted with dichloromethane (2 x 25 mL). The combined organic layer was dried over anhydrous sodum sulphate and concentrated under reduced pressure to afford the crude compound 50 (235 mg) as a brown solid, 5 which was directly taken for next reaction without further purification. 1H NMR (300 MHz, CDC13): S 9.64 (s, 1H), 8.48 (d,/ = 2.0 Hz, 1H), 7.84 (dj= 2.0, 8.8 Hz, 1H), 7.16 (dJ = 8.8 Hz, 1H), 4.34 (q, J = 6.8 Hz, 2H), 3.58 - 3.55 (m, 2H), 3.27 - 2.97 (m, 7H), 2.75 (s, 3H), 2.58 - 2.54 (m, 4H), 1.82 - 1.72 (m, 2H), 1.67 - 1.53 (m, 6H), 1.02 (t,/ = 7.3 Hz, 3H); LCMS (M+H) = m/z 564.3 [M+H+]; purity~94%. 10 EXAMPLE 86 3-(4-(4-ethoxy-3-(6-formyl-5-methyl-4-oxo-7-propyl-3,4-dihydropyrrolo[l,2- /|[l,2,4]triazin-2-yl)phenylsulfonyl)piperazin-l-yl)propyl nitrate (51) To a stirred solution of 8 (220 mg) in DMF (3 mL), AgNOs(266 mg, 1.56 mmol) and Nai (58.5 mg, 0.39 mmol) were added at room temnperature. The reaction was then heated to 15 60 °C and stirred for 48 h. The reaction mixture was then cooled to room temeparature, diluted with water (15 mL) and extracted with ethyl acetate (3 X 50 mL). The combined organic layers were dried over anhydrous sodum sulphate and concentrated under reduced pressure to afford the crude compound 50. Note: The same reaction was perfomed in two batches (100 mg + 200 mg) and the obtained 20 crude materials were combined and purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound 9 (52 mg) as a brown solid. LCMS (M+H) = m/z 591.4 [M+H+]; purity~97%. EXAMPLE 87 (E)-3-(4-(4-ethoxy-3-(6-((hydroxyimino)methyl)-5-methyl-4-oxo-7-propyl-3,4- 25 dihydropyrrolo[l,2-/|[l,2,4]triazin-2-yl)phenylsulfonyl)piperazin-l-yl)propyl nitrate (lao) To a stirred solution of 51 (50 mg, 0.084 mmol) in ethanol (2 mL) and water (0.5 mL), was added hydroxylamine hydrochloride (15 mg, 0.211 mmol) and the reaction mixture was heated to 90 °C for 12 h. After completion of reaction (monitored by TLC), the reaction 30 mixture was concentrated under reduced pressure. The obtained residue was dissolved in water (2 mL) and extracted with 5% methanol in dichloromethane (3 X 25 mL). The CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 105 — combined organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and acetonitrile to afford the title compound lao (10 mg) as a white solid. 1H NMR (400 MHz, DMSO-de): 8 11.56 (br s, 1H; D2O exchageable), 10.95 5 (s, 1H; D2O exchageable), 8.21 (s, 1H), 7.93 - 7.78 (m, 2H), 7.39 (br d,/ = 8.8 Hz, 1H), 4.48 (br t,/ = 6.6 Hz, 2H), 4.29 - 4.15 (m, 2H), 3.14 - 2.77 (m, 6H), 2.55 (s, 3H), 2.47 - 2.20 (m, 6H), 1.86 - 1.70 (m, 2H), 1.59 (tdj= 7.3, 14.7 Hz, 2H), 1.35 (t,/ = 7.1 Hz, 3H), 0.87 (t,J = 7.3 Hz, 3H); LCMS (M+H) = m/z 606.4 [M+H+]; purity~95.8%. 10 SCHEME 40: (CH2OH)2, benzene EXAMPLE 88 2-(2-ethoxy-5-((4-(3-hydroxypropyl)piperazin-l-yl)sulfonyl)phenyl)-5-methyl-7- propylpyrrolo [2,l-/][l,2,4]triaziii-4(3fr)'one (lap) 15 In a reaction tube, 2-(2-ethoxy-5-((4-(3-hydroxypropyl)piperazin-l-yl)sulfonyl) phenyl)-5- methyl-4-oxo-7-propyl-3,4-dihydropyrrolo [2,l-/][l,2,4]triazine-6-carbaldehyde (49) (150 mg, 0.275 mmol), benzene (2.5 mL) and ethylene glycol (2.5 mL) were charged and stirred for 5 min. To this, PTSA.H2O (75 mg, 0.399 mmol) was added and capped the reaction tube. The reaction mixture was dipped in a pre-heated oil bath at 140 °C and stirred for 16 h. 20 After completion of reaction (monitored by TLC and LCMS analysis), the reaction mixture was cooled to room temperature and diluted with water (20 mL). The resultant solution was extracted with ethyl acetate (2X15 mL). The combined organic layer was dried over anhydrous Na2SO4 and concentrated under reduced pressure. The obtained crude product was purified by reverse phase preparative HPLC using 0.1% formic acid in water and 25 acetonitrile to afford lap (50 mg) as a white solid. '11 NMR (400 MHz, CDCI3) 8 = 9.49 (br s, 1H; D2O exchangeable), 8.50 (d,/ = 2.4 Hz, 1H), 7.80 (dd,/ = 2.4, 8.8 Hz, 1H), 7.14 (d,/ = 8.8 CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 106 — Hz, 1H), 6.17 (s, 1H), 4.34 (q,/ = 7.0 Hz, 2H), 4.26 (br s, 1H; D20 exchangeable), 3.72 (t, / = 5.1 Hz, 2H), 3.07 (br s, 4H), 2.85 (t,/ = 7.6 Hz, 2H), 2.75 - 2.58 (m, 6H), 2.54 (s, 3H), 1.84 - 1.65 (m, 4H), 1.61 (t, J = 7.0 Hz, 3H), 1.01 (t, J = 7.6 Hz, 3H); LCMS (ESI) = m/z 518.27, purity—95.44%. 5 SCHEME 41: EXAMPLE 89 (Z)-2-(l-((3-(6-((hydroxyimino)methyI)-5-methyl-4-oxo-7-propyI-3,4-dihydropyrrolo[2,l- f][1,2,4]triazin-2-yl)-4-propoxyphenyl)sulfonyl)piperidin-4-yl)ethyl nitrate (laq) 10 Iv (100 mg; 0.164 mmol) was dissolved in benzene (2 mL) at reflux temperature under argon atmosphere. The reaction was then cooled to ~50 °C and purged with dry HC1 gas until the precipitation started to form (~5 min). After the precipitation was observed, the reaction was cooled to 0 °C and stirred for 15 min. The solid was filtered, washed with benzene (0.5 mL), petroleum ether (0.5 mL) and dried under vacuum. The solid was taken in diethyl ether (8 mL) 15 and added pre-cooled 2.6 M aqueous NaOH solution (-1.0 mL ) at 0 °C until the solid was complteley dissolved. Saturated NH4CI solution (~2.0 mL ) was added to it until the precipitate forms and within 1min, went back in to the solution. The diethyl ether layer was separated and the aqueous layer was extracted with diethyl ether (2X8 mL). The combined organic layer was dried over anyhdrous NazSCUand concentrated under vacuum 20 to afford the crude product with LCMS—66% purity. The product was purified by reverse phase Prep HPLC (XBridge Cl 8 column; lOmM aqueous ammonium bicarbonate solution with acetonitrile) to afford laq (17.5 mg) as an off-white solid. 1H NMR (400 MHz, DMSO-^) 5 11.38 (s, 1H; D2O exchangeable), 11.18 (br s, 1H, D2O exchangeable), 7.90 - 7.78 (m, 2H), 7.51 (s, 1H), 7.36 (br dj= 9.3 Hz, 1H), 4.52 (br t,/ = 6.6 Hz, 2H), 4.11 (t,/ = 6.4 Hz, 2H), 3.64 25 - 3.61 (m, 2H), 2.89 - 2.77 (m, 2H), 2.41 (s, 3H), 2.28 - 2.23 (m, 2H), 1.82 - 1.66 (m, 4H), 1.65 - 1.51 (m, 4H), 1.38 - 1.31 (m, 1H), 1.25 - 1.19 (m, 2H), 0.94 (t,/ = 7.3 Hz, 3H), 0.83 (t,/ = 7.3 Hz, 3H); LCMS (ES): m/z 605.5 [M+H+]; purity~95%. - 107- EXAMPLE 90 PDE5 assay Purpose: Evaluation of the effects of compounds of the present invention on the activity of the human phosphodiesterase-5 quantified by measuring the formation of 5’GMP from cGMP using 5 PDE5 enzyme isolated from human platelets. The latter was effected in accordance with the method as described by Masaaki I, Nishikawa M, Fujioka M, Miyahara M, Isaka N, Shiku H, Nakano T, Cell Signal (1996), 8(8):575-581. Experimental protocol: The test compound, i.e the compound of the present invention, reference compound or water (control) are added to a buffer containing 40 mM Tris/HCl (pH 7.8), 3 mM 10 MgC12, 1.4 mM DTT, 0.21% BSA, 200 mM NH4C1, 1 pM cGMP and 0.1 pCi [3H]cGMP. Thereafter, the reaction is initiated by addition of the enzyme and the mixture is incubated for 60 min at 22°C. For basal control measurements, the enzyme is omitted from the reaction mixture. Following incubation SPA beads are added. After 20 min at 22°C under shaking, the amount of [3H]5’GMP 15 is quantified with a scintillation counter (Topcount™, Packard). The results shown in Table 1 are expressed as a percent inhibition of the control enzyme activity. The standard inhibitory reference compound is dipyridamole, which is tested in each experiment at several concentrations to obtain an inhibition curve from which its IC50 value is calculated. As shown in Table 1, the compounds of the present invention are potent and selective inhibitors 20 of human cGMP-specific PDE5. Table 1 Compound % inhibition at 5.0E-09 M IC50 sildenafil 49.9 5.4xlO-9M la 77.6 2.5xlO'loM lb 34.0 1.2x10"8M Ik 24.5 In 33.0 lo 83.0 5.3x10'loM Ir 74.0 1.6xlO-loM Is 46.0 Date Regue/Date Received 2023-02-09 CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 108 — It 44.9 lu 50.3 1.5x10’9M Iv 45.9 4.1x10‘9M Iw 75.5 ly 6.4 lz 87.0 lab 86.8 lac 12.4 lad 50.5 lae 44.6 laf 74.5 lag 18.7 lah 69.7 lai 0.3 lak 8.3 lai 84.9 4.8xlO'loM lam 25.1 6.6x10'8M lan 84.9 4.2xlO’loM lao 70.6 1.2x10‘8M lap 69.2 2.8x10’9M laq 1.3 EXAMPLE 91 Measurements of cGMP in human pulmonary artery smooth muscle cells (hPASMC) Human Pulmonary Artery Smooth Muscle Cells (hPASMC) were purchased from Clonetics™ 5 Lonza (Lonza, reference number CC-2581) and cultured in Clonetics™ smooth muscle growth medium (Clonetics™ SmGM™-2 with BulletKit™ growth factor supplements (Lonza, reference number CC-3182) at 37°C in 5% CO2. Culture medium was replaced each 48 hours. Cells were grown in 75cm2 culture plates. 48 h before the experiments, cells were trypsinized (Trypsin kit One ReagentPack™ (CC-5034), 10 Lonza) and plated in 96 well plates precoated with collagen I at 10000 cells per well. 24h before the experiments culture medium was replaced by serum-reduced (0.5% FBS) medium. - 109- Immediately before the experiments, medium was exchanged and hPASMC incubated in presence of the inventive compound Iv (in concentrations of 1x10'12M (IpM) - 1x10'7M (100nM)), the inventive compound Ir (in concentrations of 1x1016M(O.lfM) — 1x10’7M (lOOnM)), the reference PDE5 inhibitor sildenafil (in concentrations of lxlO‘loM (O.lnM) - 1x10’7M (lOOnM)) or vehicle 5 (0.1% DMSO) over 15 or 30 min. Measurements of intracellular cGMP were performed using the Amersham™ cGMP EIA System (GE Healthcare, RPN226) following the instructions of the manufacturer. The assay has a sensitivity of 2 finol cGMP per well. Briefly, incubations were terminated by adding Amersham's lysis buffer 1and cells left for 10 min under agitation to ensure complete lysis. cGMP in samples 10 was then acetylated using triethylamine and acetic anhydride and determined by a competitive ELISA. The ELISA is based on the competition between acetylated cGMP in cell culture lysates and a peroxidase-labelled cGMP conjugate for limited binding sites on a cGMP specific antiserum immobilized on pre-coated 96 well MTP. cGMP was determined based on a standard curve. Results were expressed as finol cGMP in 104 cells as means +/- SE from 3 independent 15 experiments in triplicates (FIG. 1A, FIG. IB, FIG. IC). Surprisingly, the inventive compounds Iv and Ir, and in particular Ir, show a significantly higher activity as compared to the reference inhibitor sildenafil. EXAMPLE 92 20 Isometric Tension Studies on Rat Aortic Rings. Animals A total of 131male 11-week-old Sprague-Dawley rats from Harland (Barcelona) were used. The study was in line with the Guide for the Care and Use of Laboratory Animals (1996). After 1week of acclimatization, rats were anesthetized. Appropriate depth of anesthesia was determined by the 25 absence of the leg flexor response and the eyelid reflex. Then, the thorax was quickly opened by a midline incision, and the rat was sacrificed by cutting the heart and exsanguination. The aorta was quickly removed without damaging the endothelium and placed in a beaker filled with Krebs’s solution and bubbled with 95% O2 / 5% CO2. 30 Preparation of the isolated organs Rat aortic rings: Intact endothelium In brief, 3- to 4-mm thoracic aortic rings were mounted in separate 5-ml organ baths containing Krebs solution with (mM) NaCl 118.0, KC1 4.7, CaCl2 1.9, KH2PO4 1.2,MgSO4 1.2,NaHCO3 25.0 and glucose 5.0 and maintained at 37°C and bubbled with 95% O2 / 5% CO2 (Klein T, Date Regue/Date Received 2023-02-09 CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 110- Eltze M, Grebe T, Hatzelmann A, Komhoff M (2007). Celecoxib dilates guinea-pig coronaries and rat aortic rings and amplifies NO/cGMP signaling by PDE5 inhibition. Cardiovasc Res 75: 390-7). Indomethacin (1 pM) was added to the saline solution. The tissues were attached to force displacement transducers, stretched to a resting tension of 1- 5 1.5 g. Next, the endothelial integrity of the preparations was determined by verifying the responsiveness to acetylcholine (ACh, 1 pM) in vessels precontracted with phenylephrine (PE, 300 nM, corresponding to 80-90% of its maximum effect). When the relaxation by 1pM ACh of the tension achieved with 300 nM PE was more than 60%, the preparation was eligible. Eligible rings were then washed several times to restore tension to the baseline level. After this 10 procedure, the preparations were allowed to equilibrate for 60 min before contraction to a new single concentration of phenylephrine (300 nM). Rat aortic rings: Mechanically removed endothelium In some experiments, the entire length of the thoracic aorta was functionally denuded of the endothelial layer by gently scraping the luminal surface with a 1.5 mm glass rod. The tissues 15 were attached to force displacement transducers, stretched to a resting tension of 1-1.5 g. Next, the absence of the endothelium was confirmed by verifying an impaired responsiveness to acetylcholine (1 pM) in vessels precontracted with PE (300 nM). Rings were then washed several times to restore tension to the baseline level. After this response the preparations were allowed to equilibrate for 60 min before contraction to a new single concentration of 20 phenylephrine (300 nM; EC80-90 of its own maximal effect). Rat aortic rings: Endothelial dysfunction secondary to high glucose In brief, 3- to 4-rnm thoracic aortic rings were mounted in separate 5-ml organ baths containing Krebs’ solution with (mM) NaCl 118.0, KC1 4.7, CaC12 1.9, KH2PO4 1.2, MgSO4 1.2, NaHCO3 25.0 and glucose 5.0, maintained at 37°C and bubbled with 95% 02 / 5% CO2 [1], 25 Indomethacin (1 pM) was added to the saline solution. The tissues were attached to force displacement transducers, stretched to a resting tension of 1-1.5 g. Next, the endothelial integrity of the preparations was determined by verifying the responsiveness to acetylcholine (1 pM) in vessels precontracted with PE (300 nM). Only vessels with at least 60% relaxation to IpM ACh from the tension with 300 nM were eligible. 30 Eligible rings were washed and equilibrated several times to restore tension to the baseline level with Krebs solution with (mM) NaCl 118.0, KC1 4.7, CaC12 1.9, KH2PO4 1.2, MgSO4 1.2, NaHCO3 25.0 and glucose 25.0 and maintained at 37°C, 95% 02 / 5%CO2. The preparations were allowed to equilibrate for 60 min in Krebs with high glucose (25 mM) before contraction to a new single concentration of PE (300 nM). The experimental protocol was a previously - Ill - described (Dhar I, Dhar A, Wu L, Desai K (2012). Arginine attenuates methylglyoxal- and high glucose-induced endothelial dysfunction and oxidative stress by an endothelial nitric-oxide synthase-independent mechanism. J Pharmacol Exp Ther 342: 196-204) with modifications. 5 Design — Concentration dependent relaxation curves — Cumulative Protocol In the first set of experiments, concentration-response curves (1 pM - 1 pM) for Sildenafil (reference), Ir, or Iv were constructed in endothelium-intact or -denuded preparations, in the presence of N-nitro-L-arginine methyl ester (L-NAME 100 pM) or in Krebs with High Glucose (25 mM). When the influence of NG-nitro-L-arginine methyl ester (L-NAME) on the relaxation 10 induced by test and reference compounds (Sildenafil, Ir or Iv) was evaluated, L-NAME was added to the preparations 30 min prior to PE. On the plateau of PE induced tension the compounds (sildenafil, Ir or Iv) were cumulatively added (30 min per concentration) to the bath until maximum relaxation was achieved. Finally, sodium nitroprusside (0.1 mM) was added, in order to obtain maximum relaxation of arterial rings. 15 The amount of relaxation (percent values as means±SEM) was quantified as percent relaxation from the tension (plateau) achieved with phenylephrine. Drugs Compounds were dissolved in DMSO (100%) at lOmM concentrations and stored in aliquots at - 20 20°C. Dilutions were performed immediately before each experiment and the DMSO concentration kept at 0.1% in all incubations. Analysis of results Data are presented as mean±SEM. Statistical analysis of results has been performed by analysis of 25 variance (ANOVA) either parametric or non-parametric (Kruskal-Wallis), followed by Bonferroni test or Dunn tests as appropriate (GraphPad Software Inc, San Diego, CA, USA). Significance was accepted when P<0.05. Non-linear regression was conducted with GraphPad™ Software). Results are related to Vehicle control (0.1% DMSO) for each measurement point. 30 RESULTS FIG. 2 shows the concentration-dependent relaxation of phenylephrine (PE, 300 nM) precontracted rat aortic rings with intact endothelium (FIG. 2A), when rat aortic rings were pre¬ exposed to high (25mM) glucose (FIG. 2B), in presence of the nitric oxide synthase inhibitor L- NAME (FIG. 2C) and in the absence of endothelium (FIG. 2D). Date Regue/Date Received 2023-02-09 CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 112- With intact endothelium (Fig. 2A) the inventive compounds Iv and Ir are more potent and effective than Sildenafil. It is noteworthy that Iv was almost as potent and effective as lr even though the potency to inhibit PDE5 of Iv was 40-fold higher than for lr as shown in Table 1. These findings may indicate that in the course of the exposure period, lv was in part converted 5 into lr secondary of its NO release. Pre-exposure of rat aortic rings to high glucose (25mM) is described to imitate a condition of hyperglycemia-induced endothelial dysfunction due to a loss in endothelial nitric oxide generation (Dhar I, Dhar A, Wu L, Desai K (2012). Arginine attenuates methylglyoxal- and high glucose-induced endothelial dysfunction and oxidative stress by an endothelial nitric-oxide 10 synthase-independent mechanism. J Pharmacol Exp Ther 342: 196-204). FIG. 2B shows that under these conditions lv was clearly more potent and efficacious than lr. In case of the latter, the lower potency and efficacy is most likely secondary to a loss of nitric oxide from endothelial cells resulting in less activation of the cGMP producing soluble guanylate cyclase. In contrast, the superiority of lv 15 which comprises an additional ONO2 group as compared to lr is believed to be attributed to the NO release accompanying the PDE5 inhibition. These dual-pharmacology NO-releasing PDE5 inhibitors such as lv are believed to be highly beneficial for the treatment of diabetic patients. Without being bound by this theory and notion, it is believed that once in the aortic smooth muscle cell, lv is 'bio-activated' by enzymatic / non-enzymatic processes into the more potent 20 PDE5 inhibitor lr and nitric oxide. This nitric oxide restores the compromised activity of the soluble guanylate cyclase and interacts with the PDE5 inhibitor in a more than additive fashion. This notion is confirmed under conditions when endothelial nitric oxide generation is blocked by the NOS inhibitor L-NAME (Fig. 2C) or following mechanical removal of the endothelium (Fig. 2D). Compared to intact endothelium the potency and efficacy of the pure PDE5 inhibitors lr is 25 largely impaired while the NO-releasing PDE5 inhibitor lv is superior. FIG. 3 shows the non¬ linear regression analyses of the data presented in FIG. 2. Potency (EC50), Efficacy (Emax) and concentrations where 40% relaxation was achieved are summarized in Table 2. 30 CA 03001728 2018-04-11 WO 2017/085056 PCT/EP2016/077720 - 113- Table 2: EC50, IC40 and Emax for relaxation of rat aortic rings by Iv, Ir and sildenafil Condition Intact Endothelium (E + ) High GltSd^ex IC40 Emax IC40 Emax pM pM % pM pM % sildenafil 186 194 79.3 20360 826989 49.1 Ir 7.4 3.8 91.9 2795 89295 51 Iv 11.1 8.2 85.1 181 660 64.2 Condition :. L-NAME Without Eridbtfefium EC50 IC40 Emax EC50 1C40 Emax Cpd pM pM % pM pM % sildenafil 5804 95659 51.4 20580 >1000000 36.4 Ir 935 2982 60.4 5942 >1000000 39.8 Iv 267 1412 62 218 7527 48.7 Based on EC50 and Emax, Iv as compared to sildenafil (i) exerted an about 17-fold more potent 5 relaxation of PE-precontracted rat aortic rings in experiments with intact endothelium, (ii) revealed about 95-fold more potent and more efficacious in relaxation of PE precontracted rat aortic rings in the absence of endothelium, (iii) was about 22-fold more potent and more efficacious in relaxation of PE precontracted rat aortic rings in the presence of L-NAME, (iv) most importantly, revealed about 110-fold more potent and more efficacious to exert relaxation 10 of PE precontracted rat aortic rings with intact endothelium but pre-exposed to high (25mM) glucose for 60 min prior the experiment was commenced. Thus, related to the reference PDE5 inhibitor sildenafil the inventive NO releasing PDE5 inhibitor Iv achieved 40% relaxation in PE precontracted rat aortic rings (i) with intact endothelium, (ii) without endothelium, (iii) with L- NAME, (iv) with high glucose at about 24-fold, >130-fold, 68-fold, 1250-fold lower 15 concentrations, respectively. Collectively, these data strongly support the novel and inventive preferred dual pharmacology approach of the present invention as exemplified and designed for Iv, or the corresponding nitrate-ester containing PDE5 inhibitors of the present invention, to activate soluble guanylate cyclase and inhibit PDE5. Such a preferred dual pharmacology approach outperforms PDE5 20 inhibitors alone, in particular, in conditions of endothelial dysfunction and when endogenous NO generation from endothelial cells is impaired.