CA2323442C - Procede et appareil de mesure de proteines - Google Patents
Procede et appareil de mesure de proteines Download PDFInfo
- Publication number
- CA2323442C CA2323442C CA002323442A CA2323442A CA2323442C CA 2323442 C CA2323442 C CA 2323442C CA 002323442 A CA002323442 A CA 002323442A CA 2323442 A CA2323442 A CA 2323442A CA 2323442 C CA2323442 C CA 2323442C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- tip
- dispensing
- absorbance
- dispensing tip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 26
- 102000004169 proteins and genes Human genes 0.000 title description 32
- 108090000623 proteins and genes Proteins 0.000 title description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 238000011534 incubation Methods 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 14
- 230000006835 compression Effects 0.000 claims 1
- 238000007906 compression Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 abstract description 37
- 238000012360 testing method Methods 0.000 abstract description 14
- 210000002966 serum Anatomy 0.000 abstract description 13
- 108010017384 Blood Proteins Proteins 0.000 abstract description 9
- 102000004506 Blood Proteins Human genes 0.000 abstract description 9
- 238000012216 screening Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 132
- 238000002835 absorbance Methods 0.000 description 71
- 230000005855 radiation Effects 0.000 description 35
- 239000003153 chemical reaction reagent Substances 0.000 description 31
- 229940099472 immunoglobulin a Drugs 0.000 description 31
- 108010074051 C-Reactive Protein Proteins 0.000 description 20
- 102100032752 C-reactive protein Human genes 0.000 description 20
- 238000004422 calculation algorithm Methods 0.000 description 15
- 230000000694 effects Effects 0.000 description 14
- 230000003287 optical effect Effects 0.000 description 14
- 239000000835 fiber Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 239000013307 optical fiber Substances 0.000 description 8
- 238000000862 absorption spectrum Methods 0.000 description 7
- 238000012417 linear regression Methods 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 6
- 230000010354 integration Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000011179 visual inspection Methods 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- GWZYPXHJIZCRAJ-UHFFFAOYSA-N Biliverdin Natural products CC1=C(C=C)C(=C/C2=NC(=Cc3[nH]c(C=C/4NC(=O)C(=C4C)C=C)c(C)c3CCC(=O)O)C(=C2C)CCC(=O)O)NC1=O GWZYPXHJIZCRAJ-UHFFFAOYSA-N 0.000 description 3
- RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 description 3
- 238000011481 absorbance measurement Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000000149 argon plasma sintering Methods 0.000 description 3
- QBUVFDKTZJNUPP-UHFFFAOYSA-N biliverdin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(C=C2C(=C(C)C(C=C3C(=C(C=C)C(=O)N3)C)=N2)CCC(O)=O)N1 QBUVFDKTZJNUPP-UHFFFAOYSA-N 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000007493 shaping process Methods 0.000 description 3
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000010200 validation analysis Methods 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 208000036796 hyperbilirubinemia Diseases 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000020931 dietary conditions Nutrition 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- -1 i.e. Substances 0.000 description 1
- 229940028435 intralipid Drugs 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000000275 quality assurance Methods 0.000 description 1
- 238000009789 rate limiting process Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0429—Sample carriers adapted for special purposes
- G01N2035/0434—Sample carriers adapted for special purposes in the form of a syringe or pipette tip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1062—General features of the devices using the transfer device for another function for testing the liquid while it is in the transfer device
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
La présente invention concerne un appareil et un procédé de réalisation de mesures immuno-turbidimétriques des protéines plasmatiques sur un appareil utilisé pour mesurer les interférents plasmiques et sériques. Les mesures immuno-turbidimétriques sont réalisées dans un échantillon contenu dans une pipette jetable qui fait office de cuvette et de chambre de réaction. Ces caractéristiques permettent d'effectuer des tests qui ne sont pas disponibles en général dans les analyseurs chimiques, cet appareil fournissant également un système de criblage des interférents sériques et plasmiques.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7878098P | 1998-03-19 | 1998-03-19 | |
| US60/078,780 | 1998-03-19 | ||
| PCT/CA1999/000236 WO1999047261A1 (fr) | 1998-03-19 | 1999-03-19 | Procede et appareil de mesure de proteines |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2323442A1 CA2323442A1 (fr) | 1999-09-23 |
| CA2323442C true CA2323442C (fr) | 2004-06-01 |
Family
ID=22146178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002323442A Expired - Fee Related CA2323442C (fr) | 1998-03-19 | 1999-03-19 | Procede et appareil de mesure de proteines |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1064095A1 (fr) |
| JP (1) | JP2002506981A (fr) |
| CA (1) | CA2323442C (fr) |
| WO (1) | WO1999047261A1 (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6797518B1 (en) * | 2000-09-11 | 2004-09-28 | Ortho-Clinical Diagnostics, Inc. | Analysis method with sample quality measurement |
| JP4054854B2 (ja) * | 2000-10-17 | 2008-03-05 | 独立行政法人農業・食品産業技術総合研究機構 | 近赤外分光法を用いた液状試料の分析法 |
| US7250303B2 (en) | 2001-07-20 | 2007-07-31 | Ortho-Clinical Diagnostics, Inc. | Chemistry system for a clinical analyzer |
| US7402282B2 (en) | 2001-07-20 | 2008-07-22 | Ortho-Clinical Diagnostics, Inc. | Auxiliary sample supply for a clinical analyzer |
| DE10222822A1 (de) | 2002-05-21 | 2003-12-04 | Conducta Endress & Hauser | Online-Analysator |
| WO2007028233A1 (fr) | 2005-09-06 | 2007-03-15 | Nir Diagnostics Inc. | Méthode et appareil pour mesurer des substances à analyser |
| US8597208B2 (en) | 2005-09-06 | 2013-12-03 | Covidien Lp | Method and apparatus for measuring analytes |
| WO2007056869A1 (fr) | 2005-11-21 | 2007-05-24 | Nir Diagnostics Inc. | Procede et dispositif modifies pour mesurer des analytes |
| US20100015690A1 (en) | 2008-07-16 | 2010-01-21 | Ortho-Clinical Diagnostics, Inc. | Use of fluid aspiration/dispensing tip as a microcentrifuge tube |
| WO2016130962A1 (fr) | 2015-02-13 | 2016-08-18 | Abbott Laboratories | Modules de stockage automatisés pour liquides d'analyseur de diagnostic et systèmes et procédés associés |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4990075A (en) * | 1988-04-11 | 1991-02-05 | Miles Inc. | Reaction vessel for performing sequential analytical assays |
| US5403745A (en) * | 1990-04-27 | 1995-04-04 | Genzyme Corporation | Determination of analytes in biological fluids in the presence of substances interfering with assays therefor |
| US5376313A (en) * | 1992-03-27 | 1994-12-27 | Abbott Laboratories | Injection molding a plastic assay cuvette having low birefringence |
| JP3328048B2 (ja) * | 1994-02-25 | 2002-09-24 | 富士写真フイルム株式会社 | 液体の混合方法 |
-
1999
- 1999-03-19 EP EP99908715A patent/EP1064095A1/fr not_active Ceased
- 1999-03-19 JP JP2000536485A patent/JP2002506981A/ja active Pending
- 1999-03-19 WO PCT/CA1999/000236 patent/WO1999047261A1/fr not_active Ceased
- 1999-03-19 CA CA002323442A patent/CA2323442C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1064095A1 (fr) | 2001-01-03 |
| JP2002506981A (ja) | 2002-03-05 |
| WO1999047261A1 (fr) | 1999-09-23 |
| CA2323442A1 (fr) | 1999-09-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0967954B1 (fr) | DISPOSITIF POUR la détermination de la concentration des matériaux génants dans un PLASMA | |
| US6353471B1 (en) | Method and apparatus for non-destructive screening of specimen integrity | |
| US6195158B1 (en) | Apparatus and method for rapid spectrophotometric pre-test screen of specimen for a blood analyzer | |
| US20070190637A1 (en) | Apparatus for handling fluids | |
| CA2468513C (fr) | Methode d'analyse et equipement connexe | |
| EP2016390B1 (fr) | Procédé et système de détermination quantitative d'hémoglobine | |
| US6522398B2 (en) | Apparatus for measuring hematocrit | |
| US4980278A (en) | Method of effecting immunological analysis and apparatus for carrying out the same | |
| US20050037505A1 (en) | Spectroscopic method and apparatus for analyte measurement | |
| JP2003520942A (ja) | 生体試料の無試薬分析 | |
| WO2000070350A1 (fr) | Procede et dispositif de mesure rapide de hba¿1c? | |
| EP1586887B1 (fr) | Méthode spectroscopique pour le mesure de la valeur de hémoglobine totale | |
| CA2323442C (fr) | Procede et appareil de mesure de proteines | |
| EP1023583B1 (fr) | Methode pour doser des succedanes du sang | |
| WO1988001376A1 (fr) | Procede et dispositif permettant de determiner le niveau d'un analyte dans un echantillon de sang total | |
| US20020186363A1 (en) | Method and apparatus for screening plasma for interferents in plasma from donor blood bags | |
| US6995835B2 (en) | Method and apparatus for measuring analytes in blood bags | |
| CA1272618A (fr) | Sonde analytique pour appareils servant a la determination optique des especes en presence dans une solution | |
| US20020110487A1 (en) | Apparatus and method for handling fluids | |
| US7198955B1 (en) | Method and apparatus for measurement of blood substitutes | |
| CA2592218A1 (fr) | Element solide de controle et/ou d'etalonnage pour analyseur de diagnostic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20130319 |