CA1339793C - Cyclic analogs of atrial natriuretic peptides - Google Patents

Abstract

Compounds and compositions comprising synthetic analogs of Atrial Natriuretic Peptides are provided, together with methods for their production and use as natriuretics, diuretics and/or vasodilators, or as intermediates for or modulators of such useful compounds or of native Atrial Natriuretic Peptides.

Description

13 ~397~3 -s CYCLIC ANALOGS OF ATRIAL NATRIURETIC PEPTIDES

Technical Field The invention relates to the field of metabolic regulation of the cardiovascular system. In particular, it is directed to classes of compounds with natriuretic, diuretic, and/or vasorelaxant activities.

Background Art The published PCT application of the Applicants herein, w087/02674 pub~lshed 7 May 1987 de~cribes a clas~ of va~oactive peptides which are similar to those found in atrial tissue and responsible for regulation of the tension in the cardiovascular system. These compounds are synthetic cyclic and linear analogs of the cyclic native atrial natriuretic peptides (ANP). Besides disclosure of a specific class of cyclic and linear peptides which are useful for their natriuretic, diuretic, and/or vasorelaxant activities, the published application describes methods to synthesize these peptides as well as to assess their ability to regulate salt and water balance and cardiovascular tension in vivo.
As disclosed in the published application, linea~ synthetic forms of the atrial peptides can be synthesized wherein the N-terminus is supplemented with a non-peptide group which is generally hydrophobic in nature; Examples 306 and 307 of that application describe the means to synthesize such analogs.

y -2- 1 ~ 3 ~

Furthermore, the published application describes biological assays which can, in simple in vitro tests, predict the ability of the tested compounds to behave as cardiovascular regulators in vivo. The receptor-binding S assays, using competition with native ANP for binding to cultured BASM or BAE cells, was demonstrated to correlate with the results of whole mammal assays in anesthetized rats and dogs. In isolated tissue assays, the compounds, although active in vivo, were generally not active (for instance in isolated perfused rat kidney). Nevertheless, they were able to potentiate the effect of native ANP in these isolated tissues. The results in both the in vivo and in vitro assay indicated, although no particular theory is binding on Applicants, that the synthetic analogs of the invention may be effective, at least in part, due to binding of clearance receptors for native ANP.
The experimental results in the published ap-plication are cited and incorporated herein by reference.
Disclosure of the Invention Most of the synthetic analog compounds of the present invention retain a core pentapeptide sequence of amino acid residues which correspond in a defined way to the sequence AA8-AA12 of native ANPs, using the identification system from Atlas, S., et al., Nature (1984) 309:717-719 wherein the amino-terminal arginine residue is at position 1. In the known native ANPs, this core sequence is RIDRI in rat and RMDRI in human. Certain defined permutations of this sequence, including some wherein AA12 is not present, retain activity in vivo and demonstrate that the core peptide structure is a significant factor in the peptides' biological activity.
However, as explained herein, many of these compounds are not active in in vitro tissue model systems for assay of diuretic or natriuretic activities. It is likely that ~g~93 these analogs empower the function of endogenous ANP~ by blocking clearance receptor(s) for these peptides.
Thus, in one aspect, the invention is directed to a peptide compound having natriuretic, diuretic and/or vasodilator acti~ity in mammals, which has the formula:

1 y 2 8 9 10 11 12 3 Z 4 (1) wherein:
each of AA8 and AAl1 is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AAg is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of a disulfide bond, a methylene bond, a sulfide/methylene bond, an amide bond and an ester bond;
X1 is H, a peptide of from 1 to 125 amino acid residues, or the desNH2 form thereof or is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms;
X2 is a bond or peptide of 1- 2 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in XlAAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or ~R'R" wherein R' and R' are straight or branched chain alkyl (1-lOC) wherein 1-2 nonad~acent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;

~ 13s3~g3 wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids.
- In another aspect, the invention is directed to a peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:

XlAA X2-AA8-AAg~AAlo-AAll AA12 3A Z 4 (1) wherein:
each of AA8 and AAll is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AAg is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration or a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of a disulfide bond, a methylene bond, a sulfide/methylene bond, an amide bond and an ester bond;
Xl is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms other than a gene-encoded amino acid or the D-form thereof;
X2 is a bond or peptide of 1-10 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in XlAAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or ~R'R" wherein R' and R' are straight or branched chain alkyl (l-lOC) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal C

amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;
wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids.
In another aspect, the invention is directed to a peptide compound havinq natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:

XlAAyx2-AA8-AA9-AAlo-AAll-AAl2-x3AAzx4 ( 1) wherein:
each of AA8 and AA11 is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AAg is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration or a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of an amide bond and an ester bond;
X1 is H, a peptide of from 1 to 125 amino acid residues, or the desNH2 form thereof or is a hydrophobic aliphatic, aromatic, or mixed aliphatic~aromatic organic group of from 6 to 20 carbon atoms;
X2 is a bond or peptide of 1-10 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in XlAAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or NR'R" wherein R' and R' 3S are straight or branched chain alkyl (1-lOC) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a ~ i .397g3 peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridqe;
- wherein the total ring size is equivalent to S that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids.
In another aspect, the invention is directed to a peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:
XlAAyx2-AA8-AA9-AAlo-AAll-AA12-X3AAZX4 (1) wherein:
each of AA8 and AAll is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AAg is a neutral/nonpolar/larqe/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration or a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of a disulfide bond, a methylene bond, a sulfide/methylene bond, an amide bond and an ester bond;
Xl is H, a peptide of from 1 to 125 amino acid residues, or the desNH2 form thereof or is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms;
X2 is a bond or peptide of 1-10 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in XlAAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and ~;t J

~3~93 X4 is OH, NH2, NHR' or ~R'R wherein R' and R' are straight or branched chain alkyl (l-lOC) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;
wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids;
wherein one or more of the amide linkages between ad~acent amino acid residues is replaced by a linkage selected from the group consisting of -CH2NH-, -CH2-S-, -CH2CH2-, -CH=CH-, -COCH2-, -CH(OH)CH2- and -CH2SO-, and with the proviso that if A12 is not a bond, then X2 is a tripeptide, X3 cannot be a heptapeptide.
In the peptides of all of the various aspects of the invention, one or two of the amino acid residues may be replaced by the corresponding D isomer, in addition to, or instead of, AAg and, if applicable, AA12.
The invention is also directed to pharmaceutical compositions useful as natriuretics, diuretics, vasodilators and/or modulators of the renin-angiotensin-aldosterone system, which compositions containing the above-recited analog peptide compounds, including their amides and esters, and the nontoxic addition salts thereof, together with a pharmaceutically acceptable liquid, gel or solid carrier. Administration of therapeutically effective doses of these compositions can provide effective delivery of the above-recited biological activities to mammalian hosts.
Additional aspects of the present invention provide methods for producing such compounds and composi-tions, and methods for using the compounds and composi-tions as therapeutic agents.

~,, ~

Brief Description of the Drawings 1 3 3 9 ~ ~ 3 Figure 1 schematically outlines the classifica-tion of amino acids as used herein.
- Figure 2 gives a list of various compounds of the invention.
Figure 3 gives a list of various compounds which are similar to those of the invention, but which are outside its scope.

Modes of Carrying Out the Invention In accordance with the present invention, several classes of novel analogs of native Atrial Natriuretic Peptide (ANP) compounds are provided wherein these analogs are capable of exhibiting or modulating the natriuretic, diuretic and/or vasorelaxant activity of the native peptides in mammals in vivo.
The sequence of amino acid residues of the present synthetic analog compounds, including the core pentapeptide, and preferred embodiments thereof, are defined in terms of amino acids of certain characteristics of particular subclasses.
Amino acid residues can be generally subclassified into four major subclasses as follows and as shown in Figure 1.
Acidic: The residue has a negative charge due to loss of H ion at physiological pH and the residue is attracted by aqueous solution so as to seek the surface positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium at physio-logical pH.
Basic: The residue has a positive charge due to association with H ion at physiological pH and the residue is attracted by aqueous solution so as to seek the surface positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium at physio-logical pH.

.3 ~ 7 ~ 3 Neutral/nonpolar: The residues are not charged at physiological pH and the residue is repelled by aqueous solution so as to seek the inner positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium. These residues are also designated "hydrophobic" herein.
Neutral/polar: The residues are not charged at physiological pH, but the residue is attracted by aqueous solution so as to seek the outer positions in the conformation of a peptide in which it is contained when the peptide is in aqueous medium.
It is understood, of course, that in a statisti-cal collection of individual residue molecules some molecules will be charged, and some not, and there will be an attraction for or repulsion from an aqueous medium to a greater or lesser extent. To fit the definition of charged", a significant percentage (at least ap-proximately 25%) of the individual molecules are charged at physiological pH. The degree of attraction or repul-sion required for classification as polar or nonpolar isarbitrary, and, therefore, amino acids specifically contemplated by the invention have been specifically clas-sified as one or the other. Most amino acids not specifically named can be classified on the basis of known behavior.
Amino acid residues can be further subclassified as cyclic or noncyclic, and aromatic or nonaromatic, self-explanatory classifications with respect to the side chain substituent groups of the residues, and as small or large.
The residue is considered small if it contains a total of 4 carbon atoms or less, inclusive of the carboxyl carbon.
Small residues are, of course, always nonaromatic.
For the naturally occurring protein amino acids, subclassification according to the foregoing scheme is as follows (see also Figure 1).

Acidic: Aspartic acid and Glutamic acid;

Basic/noncyclic: Arginine, Lysine;

Basic/cyclic: Histidine;

Neutral/polar/small: Glycine, Serine and Cysteine;

Neutral/polar/large/nonaromatic: Threonine, Asparagine, Glutamine;

Neutral/polar/large/aromatic: Tyrosine;

Neutral/nonpolar/small: Alanine;

Neutral/nonpolar/large/nonaromatic: Valine, Isoleucine, Leucine, Methionine;

Neutral/nonpolar/large/aromatic: Phenylalanine, and Tryptophan.

The gene-encoded amino acid proline, although technically within the group neutral/nonpolar/large/cyclic and nonaromatic, is a special case due to its known ef-fects on the secondary conformation of peptide chains, and is not, therefore, included in this defined group.
Certain commonly encountered amino acids, which are not encoded by the genetic code, include, for example, beta-alanine (beta-ala), or other omega-amino acids, such as 3-amino proprionic, 4-amino butyric and so forth, alpha-aminoisobutyric acid (Aib), sarcosine (Sar), ornithine (Orn), citrulline (Cit), t-butylalanine (t-BuA), t-butylglycine (t-BuG), N-methylisoleucine (N-MeIle), phenylglycine (Phg), and cyclohexylalanine (Cha), norleucine (Nle), cysteic acid (Cya) and methionine -ll- 13~9~3 sulfoxide (MSO). These also fall conveniently into particular categories.
Based on the above definition, - Sar and beta-ala are neutral/nonpolar/small;
t-BuA, t-BuG, N-MeIle, Nle and Cha are neutral/
nonpolar/large/nonaromatic;
Orn is basic/noncyclic;
Cya is acidic;
Cit, Acetyl Lys, and MSO are neutral/polar/
large/nonaromatic; and Phg is neutral/nonpolar/large/aromatic.
See, also, Figure 1.
The various omega-amino acids are classified according to size as neutral/nonpolar/small (beta-ala, i.e., 3-aminopropionic, 4-aminobutyric) or large (all oth-ers).
Other amino acid substitutions for those encoded in the gene can also be included in peptide compounds within the scope of the invention and can be classified within this general scheme.
The nomenclature used to describe ANP analog compounds of the present invention follows the conventional practice wherein the amino group is assumed to the left and the carboxy group to the right of each amino acid in the peptide. In the formulas representing selected specific embodiments of the present invention, the amino- and carboxy-terminal groups, although often not specifically shown, will be understood to be in the form they would assume at physiological pH values, unless otherwise specified. Thus, the N-terminal H 2 and C-terminal-O at physiological pH are understood to be present though not necessarily specified and shown, either in specific examples or in generic formulas. In the peptides shown, each encoded residue where appropriate is represented by a single letter designation, corresponding ~3~979~
to the trivial name of the amino acid, in accordance with the following conventional list:

~ One-Letter Amino Acid Symbol Alanine A
Arginine R
Asparagine N
Aspartic acid D
Cysteine C
Glutamine Q
Glutamic acid E
Glycine G
Histidine H
Isoleucine Leucine L
Lysine K
Methionine M
Phenylalanine F
Proline P
Serine S
Threonine T
Tryptophan W
Tyrosine Y
Valine V

The amino acids not encoded genetically are ab-breviated as indicated above.
In the specific peptides shown in the present application, the L-form of any amino acid residue having an optical isomer is intended unless otherwise expressly indicated by a dagger superscript (t). While the residues of the invention peptides are normally in the natural L optical isomer form, one or two, preferably one, amino acid in addition to as well as instead of AAg and/or AA12, 7~3 may be replaced with the optical isomer D form (including embodiments where AAg and AA12 are both L).
Free functional groups, including those at the carboxy- or amino-terminus, can also be modified by amidation, acylation or other substitution, which can, for example, change the solubility of the compounds without affecting their activity.
In particular, it has been discovered that carboxyl terminal amide-modified analogs of Atrial Natriuretic Peptides are particularly potent and therefore preferred embodiments of the present invention. In general, the nitrogen atom of the amido group, covalently bound to the carbonyl carbon, will be NH2, -NHR', or NR~R , wherein R~ and R" are straight or branched chain alkyl or alkyl acyl of 1-lOC, preferably 1-6C, including these groups wherein 1-2 carbons are replaced by nitrogen, oxygen or sulfur atoms. Representatives of such amido groups are: -NH2, -NHCH3, -N(CH3)2, -NHCH2cH3l NHC6H5~ -NHCH2CH(CH3)2, -NHcH2cH(CH3)cH2cH3, -NHCH2CH20H, -NHCH20CH2CH3 and -N(CH3)CH2CH2SCH2CH3, among others.
In forming amidated analogs of the present invention, the analog compounds can be synthesized directly, for example using Boc-AAx-pMBHA-Resin or Boc-AAx-BHA-Resin, wherein AAX is the selected carboxy-terminal amino acid of the desired analog compound asdescribed in further detail below. Alternatively, the analog compounds of the present invention can be chemically or enzymatically amidated subsequent to peptide synthesis using means well known to the art, or prepared by standard solution-phase peptide synthesis protocols.

I ~9 7 9 3 Preferred Embodiments A. The Core Pentapeptide - The compounds of the invention all contain the pentapeptide core sequence:
AA8-AAg~AAl0-AAll AA12 wherein each of AA8 and AA11 is, independently:
a basic/noncyclic; or a neutral/nonpolar/small; or a neutral/polar/large/nonaromatic amino acid residue;
AAg is a neutral/nonpolar/nonaromatic amino acid residue in the D or L configuration;
AA1o is an acidic amino acid residue; and AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration, or is a covalent bond.
The most preferred sequence of this core is R(I/
M)DRI, wherein all residues are in the L configuration and the amino acid residues contained within the parentheses are alternatives. Next in preference are those sequences wherein only one of the R(I/M)DRI residues has been substituted by an alternative residue within the above definitions. Preferred substitutions are:
For AA8, instead of R: A, Q, N, K, L or Nle;
for AAg, instead of I/M: V, Vt, L, Lt, It, M , t-BuA, t-BuG or Cha;
for A1o, instead of D: E or Cya;
for A11, instead of R: A, Q, N, K, Orn, or Cit;
for A12, instead of I: M, Mt, V, V , L, Lt, I , N-MeIle, t-BuA or a covalent bond.
Particularly preferred are those embodiments wherein this sequence is selected from the group consist-ing of:

A(I/M)DRI RM DRI R(I/M)DRL
K(I/M)DRI RLDRI R(I/M)DRM
Q(I/M)DRI R(I/M)ERI R(I/M)DRM
- RVDRI R(I/M)DKI R(I/M)DRI
RI~DRI R(I/M)DQI R(I/M)DRV

More than one alteration from the naturally oc-curring RIDRI or RMDRI sequence is within the scope of the invention, but less preferred. Particularly favored subsets of this group include those wherein glutamic replaces aspartic as AA10, in addition to another substitution.

B. The Nature of the Rinq; Preferred Embodiments for AAy and AAz The cyclic disulfides included within the inven-tion are directly analogous to the naturally occurring ANPs, which contain 17 amino acid residue-member disulfide rings, inclusive of the two cysteine residues which provide the sulfhydryl groups for the formation of the disulfide bond. However, those embodiments of the compounds of the invention which contain the cyclic disulfide may contain either more or, much more preferably less, than 17 amino acid residues in the cyclic structure.
As indicated, the cyclic compounds of the present invention can be provided by bonding cysteine residues, or alternate amino acid residues AAy and AAz with an equivalent bond or linking group such as, for example, -CH2-CH2-. The replacement of a sulfhydryl group on the cysteine residue with an alternative group will ef-fectively replace the cysteine residue with an alternative amino acid. For example, to replace one sulfhydryl group with a -CH2- group, the cysteine residues will be replaced by the analogous alpha-aminobutyric acid. These cyclic analog peptides can be formed, for example, in accordance with the methodology of Lebl, M. and V. J. Hruby, Tetra-16 ~ 3 ~f ~ ~ 3 hedron Lett. (1984) 25:2067-2068, or by employing the procedure disclosed in U.S. Patent No. 4,161,521.
In addition to the disulfide and methylene bridges formed by two cysteines, by two alpha-amino butyric residues or by a composite of these, i.e.:

--NHCHCO _ _ _ _ _ _ _ NHCHCO--fH2-- S CH2 ICH2 --NHCHCO _ _ _ _ _ _ _ NHCHCO--CH2 CH2 CH2 lH2 --NHCHCO _ _ _ _ _ _ _ NHCHCO--, ester or amide bridges may also be formed. For example, an ester bridge may involve the -OH of serine or threonine and carboxyl of aspartic or glutamic, e.g., NHCHCO _ _ _ _ _ _ _NHCHCO

Similarly, an amide can conveniently be obtained using the side chains of lysine and aspartic or glutamic, e.g., 3 ~

C NH (CH2)4 - NHCHCO _ _ _ _ _ _ _ NHCHCO

Methods for synthesis of these bridges are found in Schiller, P.W., et al, Biochem Biophys Res Comm (1985) 127:558-564; Schiller, P.W., et al, Int J Peptide and Protein Res (1985) 25:171-177.
The amino acids which participate in the bridge, like the others which compose the peptide, may optionally be in the D-form, so long as no more than one or two residues (aside from, or in lieu of, one of AAg and AA12) are thus configured.
Thus, Ay can be any residue capable of forming a bridge--i.e., it cannot be Gly or an omega-amino straight chain acid unless it is in the N-terminal position.
However, a methylene or substituted methylene contribution to a methylene bridge or methylene/sulfide bridge may be furnished by alpha-aminobutyric, Val, Leu, Ile, and, for example, certain aromatic (hydrophobic) amino acids such as Phe. Disulfide linkage atoms are contributed by cysteine or homocysteine; participants in an ester or amide bridge are provided by Ser, Thr, Glu, Asp, Cya (this for a sulfonamide or sulfonate ester), Lys, and so forth.
If Xl is H, AAy is the N-terminal amino acid and may participate in the bridge.
Az is chosen from among amino acid residues capable of forming a bridge to AAy. It is selected from the same general group as is Ay, although, of course, if an amide or ester bridge is to be formed the functional group furnished by Az must be complementary to that of Ay.
Also, if X4 is OH, AAz is the C terminal residue and the COOH of any residue in this position can participate in the bridge.

-18- ~ 7 ~ 3 A preferred series wherein the carboxyl of the amide bridge is provided by the C-terminus include those wherein the C-terminus is Gly, beta-Ala, or an omega-amino acid of the formula H2N(CH2)pCOOH, wherein p is 3-6. The amino components are provided by the -NH2 of lysine or of ornithine. In addition, the positions of the COOH donor and NH2 donors may be reversed, provided these groups are available in the side chains of the C-terminal and N-terminal residues.
C. Preferred Embodiments for Xl Xl can be, but need not be, a hydrophobic amino acid or other hydrophobic group. When X1 is a hydrophobic moiety, AyX2 is a group wherein the intervening linkage can be conformed to a distance of about between or 4.5-15 angstroms. In this set of embodiments, particularly preferred for Xl are hydrophobic groups of 6-20 carbons, including, especially, phenylalanine or its des-NH2 form and aromatic acetyl, butyryl, or propionyl derivatives, especially naphthyl, dibenzyl, and indolylacyl derivatives as set forth below.
Thus, one class of presently preferred organic substituent groups can be represented by the general formula:
Rl-CO-wherein Rl is an organic hydrophobic group. Included in this formula are 2-substituted acetyl, 3-substituted propionyl, and 4-substituted butyryl groups, wherein the substitutions to these groups include the general class of neutral, hydrophobic mono- and polycyclic aromatic or saturated ring systems. Other classes have the general formulas Rl-O-CO- and Rl-0-. Representative examples of the preferred substituent groups include:

fluorenylmethyloxycarbonyl (FMOC) - f~ 9 3 ~ o - ~o~
~
benzyloxycarbonyl (CBZ) 10~~~

2-(2 -(6 -methoxynaphthyl)) propionyl 152-(2 -(6 -MeONAP) ~cO~\

Diphenylpropionyl (DPP) ~ O

Biphenylacetyl (BPA) Triphenylpropionyl (TPP) ~ t 7 ~
~ O

Cyclohexylacetyl (CHA) '~

3-Indolepropionyl (3-lP) O
~' .

4-Indolebutyryl (4-IB) l-Adam~ntylacetyl (AA) 1-Naphthylacetyl (1-NA) ~ 3~ 33 2-Naphthylacetyl (2-NA) l-Naphthoxyacetyl (l-NOA) ~> o 2-Naphthoxyacetyl (2-NOA) ~o~

dibenzylacetyl (DBA) ~ ~
~' [~
biq (l'-naphthylmethyl) acetyl (BNMA) 2~ .

~
2-naphthylthioacetyl (2-NTA) ~S ~

3-phenoxypropionyl (3-POP) ~p .3~ 7 ~ 3 2-Naphthoyl t2-NYL) 2-naphthoxy (2-NO) ~f' 2-naphthyl (2-NL) groups.
Other preferred embodiments for Xl include peptides of 1-6 amino acid residues or the des-NH2 forms thereof. The Xl peptide may advantageously contain a hydrophobic residue capable of being spaced 4.5-15 angstroms from AA8. In the native sequence, the X
peptide is S-L-R-R-S-S or, putatively, some of the N-terminal deleted forms, such as L-R-R-S-S, R-R-S-S, and R-S-S. In these embodiments, too, one or more of the residues may be replaced by another in its same class, e.g., R by K or another basic amino acid, L by V or -23~ c~ 7 ~ 3 another neutral/large/nonpolar amino acid, and S by G or another neutral/polar/small amino acid or by Ala. In ad-dition, Xl may be a peptide of further N-terminal deletions such as S-S, or S, also permitting substitution by comparable forms, or X1 may simply be hydrogen.

D. Preferred Embodiments for X2 In the peptides of the invention, if the portion upstream from the pentapeptide core contains a hydrophobic moiety, it should be separated from AA8 over a space of about 4.5-15 angstroms. Therefore, the nature of the preferred forms for X2 depend on whether or not a hydro-phobic residue occurs upstream (in Xl, as AAyr or in X2).
If a hydrophobic residue does not occur, X2 is preferably a bond or a peptide of 1-3 amino acid residues wherein the residues are selected from the group consisting of Ala, Gly, and Ser. If Xl contains a hydrophobe adjacent Ay, X2 is preferably a bond or a peptide of 1-2 amino acids, wherein the residues are selected from neutral/polar/small or neutral/nonpolar/small amino acids, in particular Gly, Ser, Ala, Aib, and Sar. Particular prefered forms include a bond, G, G-G, A-G, S-G, G-A, G-S, G-Aib, and G-Sar. If Ay is itself hydrophobic, prefered forms of X2 further include tripeptides wherein the residues are selected from neutral/polar/small and neutral/nonpolar/small, as above.
In the native cyclic forms, Ay is C and X2 is a tripeptide of the sequence F-G-G. When X2 includes F or other hydrophobic residue, preferred forms of X2 are those wherein the C-terminal portion of the X2 peptide provides the required spacing of 4.5-15 angstroms. Especially favored is G-G or that wherein the G-G dipeptide has one residue replaced by Ser, Ala, Aib or Sar, or wherein only one residue of G (or its substitute) is present.
However, it is clear that in these cyclic forms, no upstream hydrophobicity is necessary and the group XlAAyX2 may simply be a peptide extension.

-24- ~ 3 9 7 ~ ~ 3 E. Preferred Embodiments of X3 X3 is generally a peptide residue of such length that the ring will contain 7-17 residues, including the AAy and AAz residues if AAy and AAz are cysteines; or will form a ring of comparable size if they are not. Thus, if, for example, X2 is a tripeptide, X3 is preferably a bond or a peptide containing 1-6 amino acid residues. X3 will preferably be a peptide which is a variant of the native sequence G-A-Q-S-G-L-G or the truncated forms thereof, wherein one or more of the Gly or Ser residues may be replaced by another neutral/polar/small amino acid or by Ala, and Ala by another neutral/nonpolar/small amino acid or Gly or Ser. Gln may be replaced by another neutral/
polar/large/nonaromatic amino acid; and Leu may be replaced by another neutral/nonpolar/large/nonaromatic amino acid.
The amino acid residues included in X3 may in particular include omega-amino forms. Thus, in a preferred embodiment, X3 is a mono or dipeptide wherein one of the residues is of the formula -HN(CH2)bCO- wherein b is 3-6.
Exemplary truncated forms also include G-A-Q-S-G-L, A-Q-S-G-L-G, G-A-Q-S-G, Q-S-G-L-G, G-A-Q-S, S-G-L-G, G-A-Q, G-A-A, G-L-G, L-G, G-A, G and desX3.

F. Preferred Embodiments of X1 Preferred embodiments for X4 are (OH)NH2, NHR' wherein R' is straight or branched chain allyl of 1-lOC
wherein 1-2 C can be replaced by nonadjacent N, O, or S, or a peptide of 1-5 particularly 1-2 amino acid residues, and the amide or alkyl amide forms thereof, especially N-S-F-R-Y, N-S-F-R, N-S-F, N-S, or N and the amides thereof, and variants wherein one or more residues is replaced by another of the same class.

-25- ~ 7 ~ ~

G. Preferred Compounds Examples of preferred cyclic compounds include - (2-naphthylacetyl)-C-G-R-I-D-R-I-G-A-C-NH2 (2-naphthylacetyl)-C-G-R-I-D-R-I-C-NH2, (2-naphthylacetyl)-G-C-R-I-D-R-I-C-NH2, (2-naphthylacetyl)-G-C -R-I-D-R-I-G-C-NH2 and (2-naphthylacetyl)-G-C-R-I-D-R-I-G-A-C-NH2 where the Cys residues forming the ring can also be in the D form.
The preferred invention compounds also include cyclic compounds which are not disulfides. Exemplary preferred compounds include 2-naphthylacetyl-K-G-R-I-D-R-I-G-G, which contains an amide linkage between the K amino sidechain and the G carboxyl;

2-naphthylacetyl-K-G-R-I-D-R-I-E-NH2, which contains an amide linkage between the K amino sidechain and the E sidechain carboxyl;

2-naphthylacetyl-K-G-R-I-D-R-I-G-D-NH2, which contains an amide linkage between the K amino sidechain and the D sidechain carboxyl.

H. Non-Peptide Linkages In one embodiment of the invention, the amide linkages (-CO-NH-) within the core pentapeptide or those described above within Zl and/or Z2 and/or Z3 can be replaced with other types of linkages such as -CH2NH-, ~9~3 -CH2-S-, -CH2CH2-, -CH=CH- (cis and trans), -COCH2-, -C(OH)CH2- and -CH2SO-, by methods known in the art. The following references describe preparation of peptide analogs which include these alternative-linking moieties:
Spatola, A.F. Vega Data, (March 1983), Vol. 1, Issue 3, "Peptide Backbone Modifications" (general review);
Spatola, A.F. in ~Chemistry and Biochemistry of Amino Acids Peptides and Proteins", B. Weinstein, eds., Marcel Dekker, New York, p. 267 (1983) (general review); Morley, J.S. Trends Pharm Sci (1980) pp. 463-468 (general review);
Hudson, D. et al Int J Pept Prot Res (1979) 14:177-185 (-CH2NH-, -CH2CH2-); Spatola, A.F. et al, Life Sci (1986) 38:1243-1249 (-CH2-S); Hann, M.M. J Chem Soc Perkin Trans I (1982) 307-314 (-CH-CH-, cis and trans); Almquist, R.G., et al, J Med Chem (1980) 23:1392-1398 (-COCH2-); Jennings-White, C. et al Tetrahedron Lett (1982) 23:2533 (-COCH2-);
Szelke,M.,et al,European Publication No: 45665 02/lO/82 (-C(OH)CH2-); Holladay, M.W. et al Tetra-hedron Lett (1983) 24:4401-4404 (-C(OH)CH2-); and Hruby, V.J. Life Sci (1982) 31:189-199 (-CH2-S-).

I. Synthesis Compounds within the scope of the present inven-tion can be synthesized chemically by means well known in the art such as, e.g., solid-phase peptide synthesis. The synthesis i8 commenced from the carboxy-terminal end of the peptide using an alpha-amino protected amino acid.
t-Butyloxycarbonyl (Boc) protective groups can be used for all amino groups even though other protective groups are suitable. For example, Boc-Asn-OH, Boc-Ser-OH, Boc-Phe-OH, Boc-Arg-OH or Boc-Tyr-OH (i.e., selected ANP analog carboxy-terminal amino acids) can be esterified to chloromethylated polystyrene resin supports. The poly-styrene resin support is preferably a copolymer of styrenewith about 0.5 to 2% divinyl benzene as a cross-linking agent which causes the polystyrene polymer to be B

-27- ~ 3 completely insoluble in certain organic solvents. See Stewart, et al, Solid-Phase Peptide Synthesis (1969) W.H.
Freeman Co., San Francisco and Merrifield, J Am Chem Soc (1963t 85:2149-2154. These and other methods of peptide synthesis are also exemplified by US Patent Nos.
3,862,925, 3,842,067, 3,972,859, and 4,105,602.
The synthesis may use manual techniques or automatically employing, for example, an Applied BioSystems 430A Peptide Synthesiz~r (Foster City, California) or a Biosearch SAM II automatic peptide synthesizer (Biosearch, Inc. San Rafael, California), fol-lowing the instructions provided in the instruction manual supplied by the manufacturer.
It will be readily appreciated by those having ordinary skill in the art of peptide synthesis that the intermediates which are constructed in accordance with the present disclosure during the course of synthesizing the present analog compounds are themselves novel and useful compounds and are thus within the scope of the invention.
Alternatively, selected compounds of the present invention can be produced by expression of recombinant DNA
constructs prepared in accordance with well-known methods.
Such production can be desirable to provide large quanti-ties or alternative embodiments of such compounds. Sincethe peptide sequences are relatively short, recombinant production is facilitated.

J. Administration and Use.
Compounds of the present invention are shown to have natriuretic, diuretic and hypotensive activity in the intact mammal, and may possess vasorelaxant activity or inhibit the release of aldosterone and renin.
Thus these compounds, and compositions contain-ing them, can find use as therapeutic agents in the treat-ment of various edematous states such as, for example, congestive heart failure, nephrotic syndrome and hepatic -28- i ~.3);

cirrhosis, in addition to hypertension and renal failure due to ineffective renal perfusion or reduced glomerular filtration rate.
- Thus the present invention also provides compositions containing an effective amount of compounds of the present invention, including the nontoxic addition salts, amides and esters thereof, which may, alone, serve to provide the above-recited therapeutic benefits. Such compositions can also be provided together with physio-logically tolerable liquid, gel or solid diluents, adjuvants and excipients.
These compounds and compositions can be administered to mammals for veterinary use, such as with domestic animals, and clinical use in humans in a manner similar to other therapeutic agents. In general, the dos-age required for therapeutic efficacy will range from about 0.01 to 1000 mcg/kg, more usually 0.1 to 1000 mcg/kg of the host body weight. Alternatively, dosages within these ranges can be administered by constant infusion over an extended period of time, usually exceeding 24 hours, until the desired therapeutic benefits have been obtained.
Typically, such compositions are prepared as injectables, either as liquid solutions or suspensions;
solid forms suitable for solution in, or suspension in, liquid prior to injection may also be prepared. The preparation may also be emulsified. The active ingredient is often mixed with diluents or excipients which are physiologically tolerable and compatible with the active ingredient. Suitable diluents and excipients are, for example, water, saline, dextrose, glycerol, or the like, and combinations thereof. In addition, if desired the compositions may contain minor amounts of auxiliary substances such as wetting or emulsifying agents, stabilizing or pH-buffering agents, and the like.
The compositions are conventionally administered parenterally, by injection, for example, either subcutane--29~ t~ 7 ~ ~

ously or intravenously. Additional formulations which are suitable for other modes of administration include sup-positories, intranasal aerosols, and, in some cases, oral formulations. For suppositories, traditional binders and excipients may include, for example, polyalkylene glycols or triglycerides; such suppositories may be formed from mixtures containing the active ingredient in the range of 0.5% to 10% preferably 1%-2%. Oral formulations include such normally employed excipients as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, magnesium carbonate, and the like. These compositions take the form of solutions, suspensions, tablets, pills, capsules, sustained-release formulations, or powders, and contain 10%-95% of active ingredient, preferably 25%-70%.
The peptide compounds may be formulated into the compositions as neutral or salt forms. Pharmaceutically acceptable nontoxic salts include the acid addition salts (formed with the free amino groups) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or organic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed with the free carboxyl groups may be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like.
In addition to the compounds of the present invention which display natriuretic, diuretic or vasorelaxant activity, compounds of the present invention can also be employed as intermediates in the synthesis of such useful compounds. Alternatively, by appropriate selection, compounds of the present invention whose activ-ity levels are reduced or eliminated entirely can serve to modulate the activity of other diuretic, natriuretic or ~'~793 vasorelaxant compounds, including compounds outside the scope of the present invention, by, for example, binding to alternate receptors, stimulating receptor turnover, or providing alternate substrates for degradative enzyme or receptor activity and thus inhibiting these enzymes or receptors. When employed in this manner, such compounds can be delivered as admixtures with other active compounds or can be delivered separately, for example, in their own carriers.
Compounds of the present invention can also be used for preparing antisera for use in immunoassays employing labeled reagents, usually antibodies.
Conveniently, the polypeptides can be conjugated to an antigenicity-conferring carrier, if necessary, by means of dialdehydes, carbodiimide or using commercially avail-able linkers. These compounds and immunologic reagents may be labeled with a variety of labels such as chromophores, fluorophores such as, e.g., fluorescein or rhodamine, radioisotopes such as 125I, 35S, 14C, or 3H, or magnetized particles, by means well known in the art.
These labeled compounds and reagents, or labeled reagents capable of recognizing and specifically binding to them, can find use as, e.g., diagnostic reagents.
Samples derived from biological specimens can be assayed for the presence or amount of substances having a common antigenic determinant with compounds of the present inven-tion. In addition, monoclonal antibodies can be prepared by methods known in the art, which antibodies can find therapeutic use, e.g., to neutralize overproduction of immunologically related compounds in vivo.
The following examples are provided by way of illustration, rather than implying any limitation of the subject invention.

Examples In the experimental disclosure which follows, the amino acid sequence of chemically synthesized ANP
analog compounds are numbered from the amino-terminal arginine residue corresponding to the arginine residue found at position 1 in the native rat-derived Atrial Natriuretic Peptide sequence disclosed in Atlas, S., et al, Nature (1984) 309:717-719.

I. Chemical Synthesis of Atrial Natriuretic Peptide Analog Compounds A. Synthesis Procedures Compounds of the present invention were synthesized by solid-phase techniques performed manually or, alternatively, on an Applied BioSystems 430A Peptide Synthesizer (Foster City, California) or a Biosearch Sam II automated peptide synthesizer (Biosearch, San Rafael, California) using t-Boc amino acids in accordance with the instructions of the manufacturer.

Procedure A
Preparation of Boc-AAl---AAn-l-AAn Res Hydroxymethyl Polystyrene Ester One gram of selected Boc-AAn-O-Polystyrene-Resin (0.2-0.6 mmole/g resin) (obtainable from, e.g., Peninsula Labs, Inc.) is treated according to schedule A for in-corporation of the Boc-AAn l-OH.

Schedule A
1) Wash 3x with dichloromethane (CH2C12);
2) Treat for 1 min. with TFA:CH2Cl2:ethane dithiol (EDT) (45:50:5 by volume);
3) Treat for 20 min. with TFA:CH2C12:EDT
(45:50:5) by volume;

4) Wash 3x with CH2C12;

5) Treat 2x for 1 min. 10% (V/V) Diisoprophylethylamine (DIPEA) in CH2C12;

- 6) Wash 2x with CH2C12;

7) Wash 2x with methanol (MeOH);

8) Repeat (5-7) once;

9) Wash 3x with CH2C12;

10) Add 1-6 equivalents of preformed sym-metrical anhydride of the suitably protected Boc-amino acid dissolved in CH2C12 or dimethyl formamide (DMF)/CH2C12 (50:50 volume), (Boc-N-OH, Boc-Q-OH and Boc-R(TOS)-OH were coupled as active esters using N-hydroxybenzotriazole);

11) Wash 2x with CH2C12;

12) Wash 2x with 10~ DIPEA;

13) Wash 2x with CH2C12;

14) Wash 2x with MeOH;

15) Wash 2x with CH2C12;

16) Repeat steps (11-15) once;

17) Test by ninhydrin reaction according to Kaiser et al., Anal. Biochem. 34:595 (1970). If the coupling reaction was in-complete, repeat steps (10-16) or, alternatively, cap synthesis using N-acetyl imidazole (0.30 M in DMF) or an excess of acetic anhydride in CH2C12.

Procedure B
Preparation of Boc-AAn-p-Methylbenzhydrylamine resin The selected Boc-AAn-OH is attached to a p-Methylbenzhydrylamine (pMBHA) resin via N,N'-dicyclohexylcarbodiimide, as described below.

~ 33~793 Schedule B
1) Wash the pMBHA HCl resin;
2) Wash the resin 2x with 10% (V/V) DIPEA in ~ CH2Cl2;
3) Wash 2x with CH2Cl2;
4) Wash 2x with MeOH;
5) Wash 2x with Ch2C12;
6) Add 1-6 equivalents of preformed sym-metrical anhydride of the suitably protected Boc-amino acid dissolved in CH2Cl2, with reaction time of 0.5 - 24 hrs.

Unreacted amino groups are acetylated with 0.30M
N-acetylimidazole:DMF, or acetic anhydride:CH2Cl2. The following examples demonstrate the chemical synthesis of representative analog ANP compounds (identified as AP#) which illustrate certain aspects of the present invention.

Example 1 * APl R-S-S-C-F-G-G-R-I-D-R-I-G-A-Q-S-G-C-N-S-F-R-Y
One gm of Boc-Tyr(2BrZ)-O-Resin (0.54 meq/gm, Peninsula Labs Inc., Belmont, CA) was subjected to procedure A with the required sequence of amino acids (introduced in order as Boc-Arg(Tos)-OH, Boc-Phe-OH, Boc-Ser(Bzl)-OH, Boc-Asn-OH, Boc-Cys(4-CH3Bzl)-OH, Boc-Gly-OH, Boc-Ser(Bzl)-OH, Boc-Gln-OH, Boc-Ala-OH, Boc-Gly-OH, Boc-Ile-OH 1/2H2O, Boc-Arg(Tos)-OH, Boc-Asp(OBzl)-OH, Boc-Ile-OH 1/2H2O, Boc-Arg(Tos)-OH, Boc-Gly-OH, Boc-Gly-OH, Boc-Phe-OH, Boc-Cys(4-CH3Bzl)-OH, Boc-Ser-(Bzl)-OH, Boc-Ser-(Bzl)-OH, Boc-Arg(Tos)-OH). The protected peptidyl resin was treated with TFA:CH2C12:EDT (45:50:5 v/v/v) for 1 min., then 20 min. and washed 3 times with CH2Cl2 and 2 times with MeOH to give the TFA salt of the peptidyl resin, and dried in vacuo.
The peptidyl resin was then suspended in anhydrous hydrogen fluoride (HF) containing 10% anisole, ~ -~t~79 2% ethyl methyl sulfide for 30 min. at -10~C, and 30 min.
at 0~C. The HF was removed by evaporation under vacuum and the peptide/resin mixture was suspended in diethyl ether; The~peptide/resin mixture was washed twice with diethyl ether, once with chloroform, once with diethyl ether, once with chloroform and once with diethyl ether.
The peptide was extracted from the mixture with 2.0 M
acetic acid, diluted with H20 and lyophilized, to give the unoxidized sulfhydryl peptide.
The crude peptide was dissolved in deoxygenated 0.01 M ammonium acetate (NH40Ac), pH 7.9, to O.S mg/ml and then oxidized by dropwise addition of a slight excess of 0.01 M potassium ferricyanide (KCN) solution, stirred 20 minutes and adjusted to pH 5 with acetic acid. The peptide solution was treated with DOWEX AG3X4 anion exchange resin, filtered, diluted with H20 and lyophilized to give the crude cyclized peptide.
Purification of the peptide was achieved by desalting on Sephadex~ G-25F (Pharmacia Fine Chemicals) using 0.5 M AcOH as eluant, followed by ion exchange chromatography on CM-SepharoseD (Pharmacia Fine Chemicals) or CM-cellulose (Whatman) using an elution gradient gener-ated by addition of 300 mM NH40Ac, pH 6.5, to a solution of 10 mM NH40Ac, pH 4.5. Fractions were collected having a minimum 97% purity, as judged by reversed phase HPLC, then pooled and lyophilized from H20 several times to yield the purified APl acetate salt.

Example 2 * AP2 R-S-S-C-G-R-I-D-R-I-G-A-Q-S-G-C-~-S-F-R-Y
One gm of Boc-Tyr(2BrZ)-O-Resin (O.54 meq/gm, Peninsula Labs Inc., Belmont, CA) was sub~ected to procedure A with the required sequence of amino acids (introduced in order as Boc-Arg(Tos)-OH, Boc-Phe-OH, Boc-Ser(Bzl)-OH, Boc-Asn-OH, Boc-Cys(4-CH3Bzl)-OH, Boc-Gly-OH, Boc-Ser(Bzl)-OH, Boc-Gln-OH, Boc-Ala-OH, Boc-Gly-OH, Boc-,., ~,t -35- ~ 3 s 7 ~ ~

Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Asp(oBzl)-oH~ Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Gly-OH, Boc-Cys(4CH3Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Arg(Tos)-OH).
The protected peptidyl resin was treated with TFA:CH2C12:EDT (45:50:5 v/v/v) for 1 min., then 20 min.
and washed 3 times with CH2C12, 2 times with MeOH and dried in vacuo to give the TFA salt of the peptidyl resin.
The peptidyl resin was then suspended in anhydrous HF containing 10% anisole, 2% ethyl methyl sulfide for 30 min. at -10 C and 30 min. at O C. The HF
was removed by evaporation under vacuum and the peptide/
resin mixture was suspended in diethyl ether. The peptide/resin mixture was washed twice with diethyl ether, twice with chloroform, and twice with diethyl ether. The peptide was extracted with 2.0 M acetic acid and lyophilized, to give the unoxidized sulfhydryl peptide.
The crude peptide was dissolved in deoxygenated 0.01 M NH40Ac, pH 7.9, to 0.5 mg/ml and then oxidized by dropwise addition of a slight excess of 0.01 M KCN solu-tion, stirred for 20 minutes and adjusted to pH 5 withacetic acid. The peptide solution was treated with DOWEX
AG3X4 anion exchange resin, filtered, diluted with H20 and lyophilized to give the crude cyclized peptide.
Purification of the peptide was achieved by desalting on Sephadex~ G-25F using 0.5 M AcOH as eluant, followed by ion exchange chromatography on CM-Sepharose~
or CM-cellulose (Whatman) using an elution gradient gener-ated by addition of 300 mM NH40Ac, pH 6.5, to a solution of 10 mM NH40Ac, pH 4.5. Fractions were collected having a minimum 97% purity, as judged by reversed phase HPLC, then pooled and lyophilized from H20 several times to yield the purified AP2 acetate salt.

~ 33~7~3 Example 3 * AP3 R-S-S-C-F-G-G-R-I-D-R-I-G-A-Q-S-C-N-S-F-R-Y
One gm of Boc-Tyr(2BrZ)-O-Resin (0.54 meq/gm, Peninsula Labs Inc., Belmont, CA) was subjected to procedure A with the required sequence of amino acids (introduced in order as Boc-Arg(Tos)-OH, Boc-Phe-OH, Boc-Ser(Bzl)-OH, Boc-Asn-OH, Boc-Cys(4-CH3Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Gln-OH, Boc-Ala-OH, Boc-Gly-OH, Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Asp(OBzl)-OH, Boc-Ile-OH
1/2/H20, Boc-Arg(Tos)-OH, Boc-Gly-OH, Boc-Gly-OH, Boc-Phe-OH, Boc-Cys(4CH3Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Arg(Tos)-OH). The protected peptidyl resin was treated with TFA:CH2C12:EDT (45:50:5 v/v/v) for 1 min., then 20 min. and washed 3 times with CH2C12 and twice with MeOH to give the TFA salt of the peptidyl resin and dried in vacuo.
The peptidyl resin was then suspended in anhydrous HF containing 10% anisole, 2% ethyl methyl sulfide for 30 min. at -10~C and for 30 min. at 0~C. The HF was removed by evaporation under vacuum and the peptide/resin mixture was suspended in diethyl ether. The peptide/resin mixture was washed twice with diethyl ether, once with chloroform, once with diethyl ether, once with chloroform and once again with diethyl ether. The peptide was extracted from the mixture with 2.0 M acetic acid, diluted with H20 and lyophilized, to give the unoxidized sulfhydryl peptide.
The crude peptide was dissolved in deoxygenated 0.01 M NH40Ac, pH 8, to 0.5 mg/ml and then oxidized by dropwise addition of a slight excess of 0.01 M KCN solu-tion, stirred 20 minutes and adjusted to pH 5 with acetic acid. The peptide solution was treated with DOWEX AG3X4 anion exchange resin, filtered, diluted with H20 and lyophilized to give the crude cyclized peptide.
Purification of the peptide was achieved by desalting on Sephadex~ G-25F using 0.5 M AcOH as eluant, ~ 3 ~ ~ 7 ~ 3 followed by ion exchange chromatography on CM-Sepharose3 or CM-cellulose (Whatman) using an elution gradient gener-ated by addition of 300 mM NH40Ac to a solution of 10 mM
NH40Ac, pH 4.5. Fractions were collected having a minimum 97% purity, as judged by reversed phase HPLC, then pooled and lyophilized from H20 several times to yield the puri-fied AP3 acetate salt.

Example 4 * AP4 R-S-S-C-F-G-G-R-I-D-R-I-G-A-C-N-S-F-NH2 One gm of Boc-Phe-pMBHA resin, obtained using schedule B, was subjected to procedure A with the required sequence of amino acids (introduced in order as Boc-Ser(Bzl)-OH, Boc-Asn-OH, Boc-Cys(4-CH3Bzl)-OH, Boc-Ala-OH, Boc-Gly-OH, Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Asp(OBzl)-OH, Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Gly-OH, Boc-Gly-OH, Boc-Phe-OH, Boc-Cys(4-CH3Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Ser(Bzl)-OH, Boc-Arg(Tos)-OH). The peptidyl resin was then suspended in anhydrous HF contain-ing 10% anisole, 2% ethyl methyl sulfide for 30 min. at -10~C and for 30 min. at 0~C. The HF was removed by evaporation under vacuum and the peptide/resin mixture was suspended in diethyl ether. The peptide/resin mixture was washed twice with diethyl ether, once with chloroform, once with diethyl ether, once with chloroform and once again with diethyl ether. The peptide was extracted from the mixture with 2.0 M acetic acid, diluted with H20 and lyophilized, to give the unoxidized sulfhydryl peptide.
The crude peptide was dissolved in deoxygenated 0.01 M NH40Ac, pH 8, to 0.5 mg/ml and then oxidized by dropwise addition of a slight excess of 0.01 M KCN, stirred for 20 minutes and adjusted to pH 5 with acetic acid. The peptide solution was treated with DOWEX AG3X4 anion exchange resin, filtered, diluted with H20 and lyophilized to give the crude cyclized peptide.

-38~ J 7 ~ 3 Purification of the peptide was achieved by desalting on Sephadex~ G-25F using 0.5 M AcOH as eluant, followed by ion exchange chromatography on CM-Sepharose~
or CM-celiulose (Whatman) using an elution gradient gener-ated by addition of 300 mM NH40Ac to a solution of 10 mM
NH40Ac, pH 4.5. Fractions were collected having a minimum 97% purity, as judged by reversed phase HPLC, then pooled and lyophilized from H20 several times to yield the puri-fied AP4 acetate salt.
The following examples demonstrate the chemical synthesis of representative organic substituent group modified analog peptide compounds (identified as AP#) which illustrate certain aspects of the present invention.

Example 306 * AP306 (2-Naphthylacetyl)-G-G-R-I-D-R-I-G-A-NH2 One gm of Boc-Ala-pMBHA resin (0.4 meq/gm), obtained using schedule B, was subjected to procedure A
with the required sequence of amino acids and Amino-terminal substituent group (introduced in order as Boc-Gly-OH, Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Asp(OBzl)-OH, Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-Gly-OH, Boc-Gly-OH, 2-Naphthlyacetic acid). The protected peptidyl resin was washed 3 times with CH2C12 and 3 times with MeOH
and dried in vacuo.
The peptidyl resin was then suspended in anhydrous HF containing 10% anisole, 2% ethyl methyl sulfide for 30 min. at -10~C and for 30 min. at 0~C. The HF was removed by evaporation under vacuum and the peptide/resin mixture was suspended in ethyl ether. After transfer to a fritted funnel, the peptide/resin mixture was washed twice with ethyl ether, once with chloroform, once with ethyl ether, once with chloroform and once again with ethyl ether. The peptide was then extracted from the mixture with 2.0 M acetic acid, diluted with H20 and lyophilized.

, 3 ~

Purification of the peptide was achieved by ion exchange chromatography on CM-Sepharose~ (Pharmacia) using an elution gradient generated by addition of 100 mM
NH40Ac, pH 6.5, to a solution of 10 mM NH40Ac, pH 4.5.
Fractions were monitored at 254 nm and analyzed by reversed phase HPLC. Fractions having a minimum 97%
purity were pooled and lyophiliized from H20 several times to yield the purified AP306 ~cetate salt.

Example 307 * AP307 (2-Naphthoxyacetyl)-NH(CH2)4CO-R-I-D-R-I-NH2 One gm of Boc-Ile-pMBHA resin (0.4 meq/gm), obtained using schedule B, was subjected to procedure A
with the required sequence of amino acids and Amino-terminal substituent group (introduced in order as Boc-Arg(Tos)-OH, Boc-Asp(OBzl)-OH, Boc-Ile-OH 1/2H20, Boc-Arg(Tos)-OH, Boc-NH(CH2)4COOH, 2-Naphthoxyacetic acid).
The protected peptidyl resin was washed three times with CH2C12 and three times with MeOH and dried in vacuo.
The peptidyl resin was then suspended in anhydrous HF containing 10% anisole, 2% ethyl methyl sulfide for 30 min. at -10~C and for 30 min. at 0~C. The HF was removed by evaporation under vacuum and the peptide/resin mixture was suspended in ethyl ether. After transfer to a fritted funnel, the peptide/resin mixture was washed twice with ethyl ether, once with chloroform, once with ethyl ether, once with chloroform and once again with ethyl ether. The peptide was then extracted from the mixture with 2.0 M acetic acid, diluted with H20 and lyophilized.
Purification of the peptide was achieved by ion exchange chromatography on CM-Sepharose~ (Pharmacia) using an elution gradient generated by addition of 100 mM
NH40Ac, pH 6.5, to a solution of 10 mM NH40Ac, pH 4.5.
Fractions were monitored at 254 nm and analyzed by reversed-phase HPLC. Fractions having a minimum 97%

-40- ~ 7 3 3 purity were pooled and lyophilized from H2O several times to yield the purified AP307 acetate salt.
Following the procedures outlined in Examples 1-6 (to produce analog peptides AP1-4 and 306 and 307) with appropriate modification, the ANP analogs shown in Figure 4 are synthesized. If the compound contains two "C" residues, the ring formed by a disulfide is implied;
otherwise the ring is shown. Part A of the table shows compounds synthesized in a manner analogous to Examples 1-4; part B to Examples 306 and 307. In part B, the follow-ing abbreviations are used.

AA = Adamantylacetyl BPA = Biphenylacetyl CHA = Cyclohexylacetyl DBA = Dibenzylacetyl DPP = Diphenylpropionyl IB = Indolebutyryl IP = Indoleproprionyl NA = Naphthylacetyl NL = Naphthyl NM = Naphthylmethyl NO = Naphthoxy NOA = Naphthoxyacetyl NTA = Naphthylthioacetyl NYL = Naphthoyl POP = Phenoxypropionyl TPP = Triphenylpropionyl MeONAP = Methoxynaphthylpropionyl Starred compounds were verified as to sequence by sequence analysis.

't '~ 3 ~ 79~

II. Biological Testing: Receptor Binding Assays Biological activity data for selected analog Atrial Natriuretic Peptides (ANPs) of the invention which vere synthe~ized as di~clo~ed above are presented belo~ a~
re~ult~ of receptor binding as~ays. Correlation vith i~olated tissue and ~hole mammal bioa~ays ~as ~et forth in PCT APPLICATION W087/02674 dated 7 May 1987 Without intending to be bound by any theory, it is believed that the activity of the ANP analog compounds of the invention is due to their affinity for receptors in the kidney and other sites which are responsible for influencing the clearance of the endogenous ANPs. The following in vitro biological data show that the analog compounds of the invention compete with an iodinated na-tive ANP molecule for binding to receptors from culturedbovine aortic smooth muscle (BASM) cells, and bovine endothelial (BAE) cells. This competition is, evidently, diagnostic for the binding to the relevant clearance receptors. This correlation is confirmed by data in W087/
02674 as set forth above. In addition, the analogs of the invention show reduced cyclic GMP activity, an activity which is a hallmark of the direct biological function of ANP .
It is also postulated by the inventors some, if not many, of the peptides and peptide analogs disclosed herein will have oral activity as well.
Specific ANP receptor sites have been identified on target tissues, such as kidney, adrenal, blood vessels, and cultured cells. Napier, M.A., et al., Proc Nat Acad Sci USA (1984) 81:5946-5940 ; DeLean, A., et al., Endocrinoloqy (1984) 115:1636-1638 ; Schenk, D.B., et al., Biochem Biophys Res Comm (1985) 127:433-442. Since the binding of ANP or ANP analogs to these specific receptor sites is presumptively a prerequisite of biological activ-ity, binding of ANP analogs to these receptors isconsidered predictive of biological activity.
B

~ -42- ~l c~3~73~

An assay has been developed, generally in ac-cordance with the disclosure of Schenk, supra, and Scarborough, R.M., et al., J Biol Chem (1986) 261:12960-12964, which evaluates the ability of ANP analogs to compete with labeled native ANP for binding to cultured BASM and BAE cells. This native ANP, having the amino acid sequence:

R-S-S-C-F-G-G-R-I-D-R-I-G-A-Q-S-G-L-G-C-N-S-F-R-Y
was iodinated on the carboxy-terminal Y residue and is identified as ( I)-rANP(126-150). Analogous "competitive displacement" receptor binding assays are considered commonplace in the art for examining specific ligand-receptor interactions. 125 In this assay, 0.5 nM ( I)-rANP(126-150) is incubated in each individual sample of BASM cells in the presence of varying amounts of unlabeled rANP(126-150) or -the test compound. The concentration of unlabeled peptide at which 50% of maximal (125I)-rANP(126-150) binding is displaced is called Ki(app) and reflects receptor-binding affinity. Therefore, hypothetical peptide A, with a Ki(app)=lOOnM, displays substantially weaker interaction with a receptor than hypothetical peptide B with a Ki(app)=lOnM. Assuming these ANP analogs act at one or more ANP receptor sites, then increased receptor affinity should reflect increased biological potency.
Table 1 presents data which compare the concentrations at which analog compounds of the invention displace (125I)-rANP(126-150) binding from specific recep-tor sites on BASM or BAE cells.

~ _43- : 3~7~3 Table 1 Ki(app Pepti~e Sequence (nM) AP577 (2-NA)-C-G-R-I-D-R-I-G-A-C-NH2 30.0 AP578 (2-NA)-G-C-R-I-D-R-I-G-A-C-NH2 24.0 AP579 (2-NA)-Ct-G-R-I-D-R-I-G-A-C-NH2 35.3 AP580 (2-NA)-G-C -R-I-D-R-I-G-A-C-NH2 110.5 AP581 (2-NA)-G-C-R-I-D-R-I-G-A-C -NH2 21.2 AP582 (2-NA)-C-G-R-I-D-R-I-G-A-C -NH2 21.9 AP583 (2-NA)-C -G-R-I-D-R-I-G-A-C -NH2 109.1 AP584 (2-NA)-C -G-R-I-D-R-I-G-C -NH2 104.1 AP585 (2-NA)-G-Ct-R-I-D-R-I-G-Ct-NH2 140.0 AP586 (2-NA)-G-C -R-I-D-R-I-G-A-C -NH2 124.0 AP587 (2-NA)-C -G-R-I-D-R-I-G-C-NH2 39.5 AP588 (2-NA)-C-G-R-I-D-R-I-G-C-NH2 6.0 AP589 (2-NA)-G-C-R-I-D-R-I-G-C-NH2 73.5 AP540 (2-NA)-C-G-R-I-D-R-I-C-NH2 >400 AP541 (2-NA)-G-C-R-I-D-R-I-C-NH2 393.

AP542 (2-NA)-G-C -R-I-D-R-I-G-C-NH2 375.

-44- ~ flt7~

The compound AP543 demonstrates that the bridge need not be a disulfide:

AP543- (2-NA)-~-G-R-I-D-R-I-G-~ 8.9 This compound without a disulfide ring but wherein X
is hydrophobic is highly active.
In order to show that the receptor binding assay is specific to ANP, data which compares ANP-receptor interactions of rANP(126-150) with the unrelated peptide hormones angiotensin II, glucagon, parathyroid hormone and gamma-MSH is shown:

Peptide Ki(app) rANP(126-150) 7.50 angiotensin II >500 glucagon >500 parathyroid hormone >500 gamma-MSH >500 As shown above, only rANP(126-150) displays detectable ANP-receptor affinity. This attests to the~5 relevant ANP-specificity of this receptor.
The data in the foregoing tables show that a large representative sample of the compounds of the inven-tion demonstrate affinity in the specific receptor-binding assay described.

Claims (15)

1. A peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:

X1AAyX2-AA8-AA9-AA10-AA11-AA12-X3AAzX4 wherein:
each of AA8 and AA11 is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AA9 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of a disulfide bond, a methylene bond, a sulfide/methylene bond, an amide bond and an ester bond;
X1 is H, a peptide of from 1 to 125 amino acid residues, or the desNH2 form thereof or is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms;
X2 is a bond or peptide of 1-2 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in X1AAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or NR'R" wherein R' and R~
are straight or branched chain alkyl (1-10C) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;

wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids.

2. A peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:

X1AAyX2-AA8-AA9-AA10-AA11-AA12-X3AAzX4 wherein:
each of AA8 and AA11 is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AA9 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration or a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of a disulfide bond, a methylene bond, a sulfide/methylene bond, an amide bond and an ester bond;
X1 is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms other than a gene-encoded amino acid or the D-form thereof;
X2 is a bond or peptide of 1-10 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in X1AAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or NR'R" whrein R' and R' are straight or branched chain alkyl (1-10C) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;
wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids.

3. A peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:

X1AAyX2-AA8-AA9-AA10-AA11 -AA12 -X3AAzX4 wherein:
each of AA8 and AA11 is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AA9 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration or a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of an amide bond and an ester bond;
X1 is H, a peptide of from 1 to 125 amino acid residues, or the desNH2 form thereof or is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms;
X2 is a bond or peptide of 1-10 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in X1AAyX2 is between about

4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or NR'R" wherein R' and R' are straight or branched chain alkyl (1-10C) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;
wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids.
4. A peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, which has the formula:

XyAAyX2-AA8-AA9-AA10-AA11-AA12-X3AAzX4 wherein each of AA8 and AA11 is, independently, a basic/
noncyclic; neutral/nonpolar/small; or neutral/polar/large/
nonaromatic amino acid residue;
AA9 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration;
AA10 is an acidic amino acid residue;
AA12 is a neutral/nonpolar/large/nonaromatic amino acid residue in the D or L configuration or a covalent bond;
AAy and AAz are amino acid residues which together form a bridging bond, said bond selected from the group consisting of a disulfide bond, a methylene bond, a sulfide/methylene bond, an amide bond and an ester bond;
X1 is H, a peptide of from 1 to 125 amino acid residues, or the desNH2 form thereof or is a hydrophobic aliphatic, aromatic, or mixed aliphatic/aromatic organic group of from 6 to 20 carbon atoms;
X2 is a bond or peptide of 1-10 residues, provided the distance between the amino group of AA8 and a hydrophobic moiety occuring in X1AAyX2 is between about 4.5 and 15 A in an achievable conformation;
X3 is a bond or a peptide of 1-10 residues, and X4 is OH, NH2, NHR' or NR'R" wherein R' and R' are straight or branched chain alkyl (1-10C) wherein 1-2 nonadjacent C may be replaced by N, O, or S, or X4 is a peptide of from 1 to 20 residues, including the C-terminal amide or alkyl amide forms thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge;
wherein the total ring size is equivalent to that obtained by disulfide bridge formation between cysteine residues separated by 5-15 alpha-amino acids;
wherein one or more of the amide linkages between adjacent amino acid residues is replaced by a linkage selected from the group consisting of -CH2NH-, -CH2-S-, -CH2CH2-, -CH=CH-, -COCH2-, -CH(OH)CH2- and -CH2SO-.

5. The compound of claims 1,2,3, or 4 wherein X1 is selected from the group consisting of fluorenylmethyl oxycarbonyl, benzyloxycarbonyl, 2-(2'-(6'-methoxy naphthyl)) propionyl, diphenylpropionyl, biphenylacetyl, triphenylpropionyl, cyclohexylacetyl, 3-indolepropionyl, 4-indolebutyryl, 1-adamantylacetyl, 1-naphthylacetyl, 2-naphthylacetyl, 1-naphthoxyacetyl, 2-naphthoxyacetyl, dibenzylacetyl, bis (1'-napthylmethyl) acetyl, 2-naphthyl thioacetyl, 3-phenoxypropionyl, 2-naphthoyl, 2-naphthoxy, 2-naphthyl and des-NH2-phenylalanyl.

6. The compound of claim 1, 3 or 4 wherein X1 is hydrogen or a peptide of from 1 to 6 amino acid residues, said peptide selected from the group consisting of:
S-L-R-R-S-S, L-R-R-S-S, R-R-S-S, R-S-S, S-S, and S, and the foregoing wherein one residue is replaced by another residue of its same subclass.

7. The compound of claim 1, 3 or 4 wherein X2 is selected from the group consisting of a bond, and a peptide of 1-2 amino acids which are of the subclasses neutral/polar/small or neutral/nonpolar/small, or is F-(AA)a wherein a is 1 or 2 and M is of the subclasses neutral/polar/small or neutral/nonpolar/small.

8. The compound of claim 1-4 wherein X3 is selected from the group consisting of:
G-A-Q-S-G-L-G, G-A-Q-S-G-L, A-Q-S-G-L-G, G-A-Q-S-G, Q-S-G-L-G, G-A-Q-S, S-G-L-G, G-A-Q, G-A-A, G-L-G, L-G, G-A, G and a bond, and the foregoing wherein one residue is replaced by another residue of the same subclass; and -NH(CH2)bCO- wherein b is 3-6.

9. The compound of claim 1-4 wherein X4 is (OH) NH2, NHR' wherein R' is straight or branched chain allyl of 1-10C, wherein 1-2 nonadjacent C can be replaced by N, O, or S, or is a peptide of 1-5 amino acid residues or the amide or alkylamide thereof; or X4 is not present if the C-terminal carboxyl is a part of the bridge.

10. The compound of claim 1-4 wherein AA8-AA9-AA10-AA11-AA12 is R(I/M)DRI or at most one residue therein is replaced by substituting A, Q, N, K, L or NMeIle for R as AA8' V, V~, L, L~, I~, M~, t-BuA, t-BuG or Cha for I
or M as AA9;
E or Cya, for D as A10;
A, Q, N, K, Orn or Cit for R as A11; and M, M~, V, V~, L, L~, I~, N-MeIle, t-BuA or a covalent bond for I as AA12.

11. The compound of claim 1,2,3 or 4 wherein AA8-AA9-AA10-AA11-AA12 is selected from the group consisting of:

A(I/M)DRI RM~DRI R(I/M)DRL
K(I/M)DRI RLDRI R(I/M)DRM
Q(I/M)DRI R(I/M)ERI R(I/M)DRM~
RVDRI R(I/M)DKI R(I/M)DRI~
RI~DRI R(I/M)DQI R(I/M)DRV
and R(I/M) DRI.

12. The compound of claim 1,2,3 or 4 which is selected from the group consisting of that shown in Figure 2.

13. A composition useful as a natriuretic, diuretic and/or vasodilator comprising a therapeutically effective amount of the compound of claim 1,2,3 or 4 together with a pharmaceutically acceptable carrier.

14. For use in inducing natriuresis, diuresis, or vasodilatation in a mammalian host, by administration to said host, a pharmaceutically efective amount of the composition of claim 13.

15. A process for production of a peptide compound having natriuretic, diuretic and/or vasodilator activity in mammals, said peptide compound having the formula of the compound of claim 1,2,3 or 4 or the pharmacologically acceptable salts thereof, which process comprises the following steps:
a. preparing a protected peptide bonded to a solid resin carrier in a reaction mixture, wherein the peptide has an amino acid sequence of the compound of claim 1,2,3, or 4 b. removing the solid resin carrier from the peptide and deprotecting the peptide;
c. optionally forming a ring compound from the peptide;

d. optionally modifying the peptide to add any desired organic substituent groups as recited above; and e. isolating the peptide from any reaction mixture, and optionally, converting the polypeptide into an acid addition salt thereof.