CA1285282C - Thietanyl-substituted amides - Google Patents

Abstract

ABSTRACT
This invention is directed to food sweeteners of the formula:
wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms;
A' is hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms;
2 is -CH2CH2-; -CH=CH; -CH(OH)-CH2; -CH2O or ; and Y is thietanyl or alkyl-substituted thietanyl containing up to a total of 8 carbon atoms;
B' is H or an amino protecting group with the proviso that when Z is , B.' is not H.
and food acceptable salts thereof.

Description

852a~

1 THIETANYL-SUBSTITUTED AMID~S

FIEL~ OF THE I~VE~'TION
This invention relates to a novel sroup of compounds and more particularly to a novel croup of com?ounds particularly well suited as sweeteners in edible foodstuff.
DESCRIPTION OF T~E PRIOR ART
Sweetnes- is one of the primary taste cravings of both animals and humans. Thus, the utilization of sweeteninq agents in foods in order to satisfy this sensory desire is well established.
Naturally occurinc carbohydrate sweeteners such as sucrose, are still the most widely used sweetening agents. ~'hile these natu-211y occurring car~ohydrates, i.e., sugars, generally ful'ill the requirements of sweet taste, the abundant usa~e thereof does not occur without deleterious conse-quence, e.g., high caloric intake and nutritional imbalance. In fact, often times the le~cl o-- ;';ese sweeteners required in foodstuffs is far sreate~
than the level of the sweetener that is desired or economic, dietetic or other functional consideration.
i In an attempt to eliminate the disadvantages concomitant with natural sweeteners, considerable research and expense have been devoted to the produc-tion of artificial sweeteners, such as for example, saccharin, cyclamate, dihydrochalcone, aspartame, etc.
~!hile some of these artificial sweeteners satisfy the 3 requirements of sweet taste without caloric input, and have met with considerable commercial success, they : .

.. ~. . .

. . .

~.~85X82 are not, however, without their own inherent disadvantages.
For example, many of these artificial sweeteners have the disadvantages of high cost, as well as delay in the per-ception of the sweet taste, persistent lingering of the sweet taste, and very objectionable bitter, metallic aftertaste when used in food products.

Since it is believed that many disadvantages of arti-ficial sweeteners, particularly aftertaste, is a function of lo the concentration of the sweetener, it has been previously suggested that these effects could be reduced or eliminated by combining artificial sweeteners such as saccharin, with other ingredients such as aspartame or natural sugars, such as sorbitol, dextrose, maltose, etc. These combined pro-ducts, however, have not been entirely satisfactory either.
Some U.S Patents which disclose sweetener mixtures include for example, U.S. Patent No. 4,228,198; U.S. Patent No.
4,158,068; U.S. Patent No. 4,154,862; and U.S. Patent No.
3,717,477.
Accordingly, much work has continued in an attempt to develop and identify compounds that have a sweet taste and j which will satisfy the need for better lower calorie sweeteners. Search continues for sweeteners that have intense sweetness, that is, deliver a sweet taste at low use levels and which will also produce enough sweetness at low levels to act as sole sweetener for most sweetener appli-cations. Furthermore, the sweeteners sought must have good temporal and sensory qualities. Sweeteners with good tem-poral qualities produce a time-intensity sweetness response similar to natural sweeteners without lingering. Sweeteners with good sensory qualities lack undesirable off tastes and aftertaste. Furthermore, these compounds must be economical and safe to use.

In U.S. Patent No. 3,798,204, L-aspartyl-O-t-butyl-L-serine methyl ester and L-aspartyl-O-t-amyl-L-serine methyl ester are described as sweet compounds having significant sweetness.

In U.S. Patent No. 4,448,716 metal complex salts of dipeptide sweeteners are disclosed. In the background of this patent a generic formula is described as an attempt to represent dipeptide sweeteners disclosed in five prior patents: U.S. Patent No. 3,475,403; U.S. Patent No.
3,492,131: Republic of South Africa Patent No. 695,083 published July 10, 1969: Republic of South Africa Patent No.
695,910 published August 14, 1969: and German Patent No.

2,054,554. The general formula attempting to represent these patents is as follows:
H2N - CH - C0~H - CH - COOR

CE~2COOH (C"2) L L

wherein R represents the lower alkyls, lower alkylaryls and cycloalkyls, n stands for integers 0 through 5, R1 repre-sents (a) phenyl group, (b) lower alkyls, (c) cycloalkyls, (d) R2.

Where R2 is hydroxy, lower alkoxy, lower alkyl, halo-gen, (e) (S(O)m) lower alkyl where m is 0, 1 or 2 and pro-vided n is 1 or 2, (f) R3.

~.Z8528~
.

1 ~here R3 represents an hydroxy or alkoxy and (g) single or double unsaturated cycloal.~yls with up to eight carbons. These com?ounds also are not entirely satis'actory in ?rocucing a hish quality sweetness or in ?roducing a st~eet esponse at lower le~els of sweetener.
DiDeptices or aspartyl-c~ysteine and aspartyl-methionine methyl esters are disclosed by Brussel, Peer and van der Heijden in Chemical Senses and Flavour, 4, 10 141-1;2 (1979) znd in Z. Lebensm. Untersuch-Forsch, 159, 337-343 (1975). ~he authors cisclose the followinq dipeptides:

~ -L-Asp-L-Cys(."e)-OMe ~ -L-As?-L-Cys(rt)-O.`le -L-~.s?-L-Cvs~Pr)-O'Ie -L-As?-L-Cys(i-Dr)-O:le ~-L-AsD-L-Cys(t-But)-O:~e ~ -L-As?-L-Met-O.le In U S. Patent ~o 4,399,163 to Brennan, et al.
sweeteners having the following formulas are disclosed:

HOOC - CH2 - CH ~ ~ NH-----Ra C CHCONHR
O .. ..
and physiologically acceptable cationic and acid addition salts thereof wherein Ra is CH20H or CH20CH3;

., _.

`4 ~

l.J~S~

R is a branched member selected 'rom the sroup consistin~ of ~enchyl, diiso?ropylcar~inyl, d-methyl-t-butylcarbinyl, d-e'hyl-t-but~ c~rbinyl, 2-methyl~.io-2,4-cime~hyl-?en.an-3-yl, Zi-tobutyl-ca-binyl, (C H)1,~
0 ~ (cH~

P.~
~ CHI~"

~ ~
_ l C ~ ", p ~
_~IC~Ih " R
CH~
OH
R~
_~3 3o ~1 28528X

In a related patent, U.S. Patent No. 4,411l925, Brennan, et al. disclose compounds of the above general formula with R being defined hereinabove, except Ra is defined as methyl, ethyl, n-propyl or isopropyl.
U.S. Patent No. 4,375,430 to Sklavounos discloses dipeptide sweeteners which are aromatic sulfonic acid salts of L-aspartyl-D-alaninoamides or L-aspartyl-D-serinamides.
European Patent Application No. 95772, published December 7, 1988, to Tsau describes aspartyl dipeptide sweeteners of the formula:

H2N - CHCONHCIH - C02R' wherein R' is alkyl of 1 to 6 carbons, and R2 is phenyl, phenylalkenyl or cyclohexylalkenyl, wherein the alkenyl group has 1 to 5 carbons. Closely related is U.S.
Patent No. 4,439,460 to Tsau, et al. which describes dipeptide sweeteners of the formula:

CIH2 (IH2)n f~ COOH R2 wherein n is an integer from 0 to 5, and Rl is an alkyl, alkylaryl or alicyclic radical. Similar such compounds are described in many related patents, the major difference being the definition of R2.

1285;~82 In U.s. Patent No. 3,978,034 to Sheehan, et al. R2 is defined as cycloalkenyl or phenyl. U.S. Patent No.

3,695,898 to Hill defines R2 as a mono- or di-unsaturated alicyclic radical. Haas, et al. in Patent No. 4,029,701 define R2 as phenyl, lower alkyl or substituted or unsub-stituted cycloalkyl, cycloalkenyl or cycloalkadienyl, or S()m lower alkyl provided that n is 1 or 2 and m is 0 or 2.
Closely related are U.S. Patent Nos. 4,448,716; 4,153,737;

4,031,258; 3,962,468; 3,714,139, 3,642,491; and 3,795,746.

U.S. Patent No. 3,803,223 to Mazur et al. describe dipeptide sweeteners and anti-inflammatory agents having the formula:

HOOC - CH2CH - c - NRR' wherein R is hydrogen or a methyl radical and R' is a radi-cal selected from the group consisting of alkyl, or A~k - Y, wherein Alk is a lower alkylene radical, X is hydrogen or hydroxy, and Y is a radical selected from the group consis-ting of cyclohexyl, naphthyl, furyl, pyridyl, indolyl, phenyl and phenoxy.

Goldkamp, et al. in U.S. Patent No. 4,011,260 describe sweeteners of the formula:

O R
CH2 - CH - C - N - Alk - R' wherein R is hydrogen or a lower alkyl radical, Alk is a lower alkylene radical and R' is a carbocyclic radical.
Closely related is U.S. Patent No. 3,442,431.

r ' ~

U.S. Patent No. 4, 423, 029 to Rizzi describes sweeteners of the formula:
~acc o ~ ~ R
~lR2 H
wherein R is C4-C9 straight, branched or cyclic alkyl, and wherein carbons a, b and c have the (S) configuration.

European Patent Application 48,051, published March 24, 1982, describes dipeptide sweeteners of the formula:

0 H 0 0 = C - OR
M0 - C - CH2 -*CI - C - N - *C - H
N - H H R
C = O
N - H
H - C = 0 wherein M represents hydrogen, ammonium, alkali or alkaline earth, R represents ~ -C~2 ~ , -CX

2 ~2 ; 35 .. ~, ~ 285282 g Rl represents methyl, ethyl, propyl;
R2 represents -OH, or CH3;
* Signifies an L-optical configuration for this atom.
Dutch Patent Application No. 7207426, published January 16, 1973, discloses L-aspartyl-3-fenchylalanine methyl ester as a sweetening agent.
U.S. Patent No. 3,971,822 to Chibata, et al., disclose sweeteners having the formula:

R

HOOC - CH2 y H

O O

wherein R' is hydrogen or hydroxy, R2 is alkyl of one to five carbon atoms, alkenyl of two to three carbon atoms, cycloalkyl of three to five carbon atoms or methyl cycloalkyl of four to six carbon atoms and Y is alkylene of one to four carbon atoms.
U.S. Patent No. 3,907,366 to Fujino, et al.
discloses L-aspartyl-aminomalonic acid alkyl fenchyl ' diester and its physiologically acceptable salts as useful sweeteners. U.S. Patent No. 3,959,245 discloses the 2-methyl cyclohexyl analog of the above-mentioned patent.
U.S. Patent No. 3,920,626 discloses N-d~-L-aspartyl derivatives of lower alkyl esters of O-lower-alkanoyl-L-serine, ~-aline, ~-aminobutyric acid and D-~ -amino-butyric acid as sweeteners.

;

~.;285282 Miyoshi, et al. in sulletin of Chemical society of Ja~an, 51, p. 1433-1440 (1978) disclose compounds of the following formula as sweeteners:

HOOC - CHz Rl' wherein R' is H, CH3, CO2CH4, or benzyl and R2 is lower alkyl or unsubstituted or substituted cycloalkyl. European Patent Application 128,654, published December 19, 1984, describes gem-diaminoalkane sweeteners of the formula:

H3N - CH - CONH - C - NH - COR"
(IHz)m R' wherein m is 0 or 1, R is lower alkyl (substituted or unsubstituted), R' is H or lower alkyl, and R" is a branched alkyl, alkylcycloalkyl, cycloalkyl, polycycloalkyl, phenyl, or alkyl-substituted phenyl, and physically acceptable salts thereof.
U.S. Patent No. 3,801,563 to Nakajima, et al. disclose sweeteners of the formula:

COOH COOR' ICH2 ( 1CH2) n HzN - CH - CONH - CH - COOR2 wherein R' is a branched or cyclic alkyl group of 3 to 8 carbon atoms, R2 is a lower alkyl group of 1 to 2 carbon atoms and n is a integer of 0 or 1.

European Patent Application 34,876, published September 2, 1981, describes amides of L-aspartyl-D-amino acid .285Z82 dipeptides of the formula:

~NH2 j~CHr CH ~NH ~,a COOH --d ~ CHCONHR

wherein Ra is methyl, ethyl, n-propyl or isopropyl and ~ is a branched aliphatic, alicyclic or heterocyclic member which lo is branched at the alpha carbon atoms and also branched again at one or both of the beta carbon atoms. These com-pounds are indicated to be of significant sweetness.

In the Journal of Medicinal Chemistry, 1984, Vol. 27, lS No. 12, pp. 1663-8, are described various sweetener dipep-tide esters, including L-aspartyl- ~- aminocycloalkane methyl esters.' The various dipeptide esters of the prior art have been characterized as lacking significant stability at low pH
values and/or thermal stability. These characteristics have limited the scope of use of these sweeteners in food pro-ducts which are of low pH values or are prepared or served at elevated temperatures.

Accordingly, it is desired to find compounds that pro-vide quality sweetness when added to foodstuffs or pharma-; ceuticals at low levels and thus eliminate or greatly diminish the aforesaid disadvantages associated with prior art sweeteners.

~;
i ...
, ~.~85Z82 The present new compounds are amides of aspartic acid and certain amines which are characterized by the presence of a thietanyl substituent and are low caloric sweeteners possessing a high order of sweetness with pleasing taste and a high order of stability to acid pH and elevated temperatures compared to known dipeptide sweeteners.
This invention provide~ new sweetening compounds represented by the formula:

B'H N - CH - CONH - C(A)(A') - Z - Y

wherein A is hydrogen, alkyl containing 1-3 carbon atom~, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms:
A' i9 hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms:

" 35 ~ ~85Z82 .

Z i s --CH 2 CH 2 -; --CH= CH; --Cl--CH 2; --CH ( OH ) - CH 2;

-CH2O or -C-O; and Y is thietanyl or alkyl-substituted o thietanyl containing up to a total of 8 carbon atoms;
B' is H or an amino protecting group with the proviso that when z is -C-CH2, B' is not H and food acceptable o salts thereof.
In accordance with another embodiment of the present invention there is provided a compound of the formula: L
H2N--C--CONH--C(A)(A~)--z--Y
C~12 co2~l wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms; A' is hydrogen or alkyl containing 1-3 carbon atoms; A and A' taken together with the carbon atom to which they are attached form cycloalkyl con-taining 3-4 carbon atoms; Z is -CH=CH-; and Y is thie-tanyl or alkyl-substituted thietanyl containing up to a 2s total of 8 carbon atoms; and food acceptable salts thereof.
In accordance with a further embodiment of the present invention there is provided a.compound of the formula-~HN--CH--CONH--C(A)(A')--Z--Y

,:
wherein A is hydrogen, alkyl containing 1-3 carbon :.

~,,--.,;

~.~8S28~
-13a -atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms; A' is hydrogen or alkyl containing 1-3 carbon atoms; A and A' taken together with the carbon atom to which they are attached form cycloalkyl con-taining 3-4 carbon atoms; Z is -C-CH2-; Y is thietanyl o or alkyl-substituted thietanyl containing up to a total lo of 8 carbon atoms; B' is an amine protecting group; and food acceptable salts thereof.
In accordance with a still further embodiment of the present invention there is provided a compound of the formula:
H2N--C--CONH--C(A1(A')--Z--Y
C~

wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms; A' is hydrogen or alkyl containing 1-3 carbon atoms; A and A' taken together with the carbon atom to which they are attached form cycloalkyl con-taining 3-4 carbon atoms; Z is -CbH-CH2; Y is thietanyl H

or alkyl-substituted thietanyl containing up to a total of 8 carbon atoms; and food acceptable salts thereof.
In accordance with another embodiment of the present invention there is provided a compound of formula:
;I L
H2N--C--CONII--C(A)(~')--Z--Y

S~
;~

~.~85282 -13b -wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms; A' is hydrogen or alkyl containing 1-3 carbon atoms; A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms; Z is -~-o; Y is thietanyl or alkyl-substituted thiethanyl containing up to a total of 8 carbon atoms; and food acceptable salts thereof.
Description of the Preferred Embodiments In accordance with the present invention, the preferred compounds are those wherein the thietanyl ; 15 moiety is substituted with at least one lower alkyl group, preferably a beta-position on the thietanyl ring, i.e., the ~ or ~' carbon atoms. Among these the preferred thietanyl moieties disubstituted in the beta position with alkyl group. Particularly preferred are thietanyl groups which are alkyl substituted in the ~ , and ~ ', ~' positions, i.e., tetramethyl thietanyl. of the alkyl groups, the most preferred is methyl. Thus, preferred thietanyl groups include 2,4-dimethyl-; 2,2-dimethyl-; 2,2,4-trimethyl-; 2,4,4-trimethyl-; 4,4-dimethyl-; ~-isopropyl-; ~ , ~'-diethyl-; ~-tertiary-butyl-; and 2,2,4,4-tetramethyl-thietanyl groups. In all cases, the thietanyl moieties may contain up to a total of 8 carbon atoms including the four ring carbon atoms. The preferred thietanyl groups are attached to the remainder of the present new compounds at the 3-position.

, . . .
;~ ~

, . , : .

~5~82 1 Also preferred are comp~und~ in which Z
i~ -CH2-CH2-, -CH=CH-; -CH(OH)-CH2- and -C-O-.
"

O

Of these, compounds in which Z i~ -CH=CH- are also useful intermediates for preparing those in which Z is -CH2-CH2-. Compound~ in which Z i~ -C-CH2-,.
o are also useful intermediates for preparation of compounds in which Z is -CH(OH)-CH2-. When Z is -C-CH2, it is preferred that B ' is an amino protecting o group in order to avoid any possible internal cyclization with the amino group. In both cases, such preparations are effected by known reduction techniques;
When Z i5 other than -C-CH2, it is preferred that B' is H.
The amino protecting group representative of the substituent B' in Formula I is an electron-withdrawing protecting group. Exemplary protecting groups include COCF3, COOC13, and CONAr-X, wherein Ar is aryl, X is NO2, CN, COOR", COR", SO2R", halo, carboxy, SO38, SO3R", SO2NR"R", SO2NH R", SO2NH2, CONR"R", CONHR", CONH2, SOR", O-C-R", OR", OSO2R", OCF3, CH2OR", CH(OR")2, COCF3, CF3, O

CH2CF3' CC13, CtF2t+l, and the like;
wherein each R" is the same or different and is Cl-C12 alkyl and t is an integer from 1-6.
Preferable X groups are CN, COOC2H5, COOCH3, SO2CH3 or COCH3 groups.
The term aryl when used hereinabove signifies a 6-10 membered aromatic ring compounds and includes phenyl, c~ -naphthyl, ~ -naphthyl and the like.

85~
.

These novel compounds are effective sweetness agents when used alone or in combination with other sweeteners in an ingesta, e.g., foodstuffs or pharmaceuticals, For example, other natural and/or artificial sweeteners which may be used with the novel compounds of the present inven-tion include sucrose, fructose, corn syrup solids, dextrose, xylitol, sorbital, mannitol, acetosulfam, thaumatin, invert sugar, saccharin, thiophene saccharin, meta-aminobenzoic acid, meta-hydroxybenzoic acid, cyclamate, chlorosucrose, dihydrochalcone, hydrogenated glucose syrups, aspartame (L-aspartyl-L-phenylalanine methyl ester) and other dipeptides, glycyrrhizin and stevioside and the like. These sweeteners when employed with the sweetness agents of the present invention, it is believed, could produce synergistic sweet-ness responses.
Furthermore, when the sweetness agents of the present invention are added to ingesta, the sweetness agents may be added alone or with nontoxic carriers such as the above-mentioned sweeteners or other food ingredients such as acidulants and natural and artificial gums. Typical food-stuffs, and pharmaceutical preparations, in which the sweet-ness agents of the present invention may be used are, for example, beverages including soft drinks, carbonated bever-ages, ready to mix beverages and the like, infused foods (e.g. vegetables or fruits), sauces, condiments, salad dressings, juices, syrups, desserts, including puddings, gelatin and frozen desserts, like ice creams, sherbets, icings and flavored frozen desserts on sticks, confections, toothpaste, mouthwash, chewing gum, cereals, baked goods, intermediate moisture foods (e.g. dog food) and the like.

In order to achieve the effects of the present inven-tion, the compounds described herein are generally added to the food product at a level which is effective to ... ... .. . . . ... .... ..

~.;285282 perceive sweetness in the foodstuff and suitably is in an amount in the range of from about 0.0005 to 2% by weight based on the consumed product. Greater amounts are operable but not practical. Preferred amounts are in the range of from about o.OOl to about 1% of the foodstuff. Generally, the sweetening effect provided by the present compounds are experienced over a wide pH range, e.g. 2 to lo preferably 3 to 7 and in buffered and unbuffered formulations.

It is desired that when the sweetness agents of this invention are employed alone or in combination with another sweetener, the sweetener or combination of sweeteners pro-vide a sucrose equivalent in the range of from about 2 weight percent to about 40 weight percent and more prefer-ably from about 3 weight percent to about 15 weight percent in the foodstuff or pharmaceutical.

A taste procedure for determination of sweetness merely involves the determination of sucrose equivalency. Sucrose equivalence for sweeteners are readily determined. The amount of a sweetener that is equivalent to a given weight percent sucrose can be determined by having a panel of tasters taste solutions of a sweetener at known concentra-tions and match its sweetness to standard solutions of sucrose.

1~ ~85282 .
1 In order to prepare compounds of the present invention, sevecal reaction schemes may be employed.
The general reaction scheme involves amide formation between an acylating derivative of aspartic acid and amines of Formula II:

H2N - C(A)(A') - Z - Y

wherein A, A'~ Z and Y have the same meaning as previously deacribed. Acylating derivatives of aspartic acid are well-known and include, for example, aspartic anhydride, including mixed anhydrides with lower alkanoic acids and half-esters of aspartic acid.
In the amide-forming reactions, it is preferred to employ protecting groups which preclude undesired side reactions as exemplified in the following sequence:

L
B'_ NH - CH - CO2H + H2N - C(A)(A') - Z - Y

- III II

L A' B'- NH - CH - CONH - C - A
CH2 Z _ y IV

~.~85282 .
-~8-1 In these, srcup B'is an amino protecting group, B is a ca.boxyl ?rotecting group and the remaining ~roups have the same meaning as ?reviously descri~ed. A
variety o. ~rotec_ing grou?s known in the art may be em?loyed. Exam?les of many of these ~ossible groups may be found in "Protective Grou?s in Organic Synthesis"
by T.W. Green, John Wiley and Sons, 1981. Among the preferred groups that may be employed are benzyloxy-car~onyl for B'and benzyL for 3. When A includes a 'ree hydroxy group suitable ?rotecting groups can be employed as known in the art.
Coupling of compounds with general formula II
to compounds having general 'ormula III employs estabLished amide-formins techniques. One such technique uses dicyclohexylcarbodiimide (DCC) as the coupling agent. ~he DCC method may be employed with or without additives such as 4-dimethyl-aminopyridine or copper(II~. ~he DCC coupling reaction senerally proceeds at room temperature, however,-it may be carried out from about -20 to 50C. in variety of solvents inert to the reactants.
Thus suitable solvents include, but are not limited to, ~ -dime~hylformamide, methylene chloride, toluene and the like. Preferably the reaction is ca:ried out under an inert atmosphere such as argon o: nitrogen. Coupling usually is complete within 2 hours but may take as long as 24 hours depending on reactants.
3o ,:
i 1.;~85282 .

Various other amide-forming methods can be employed to prepare the desired compounds using suitable derivatives of the free-carboxy group in compounds of structure II, e.g., acid halide, mixed anhydride with acetic acid and similar derivatives. The following illustrates such methods using aspartic acid as the amino dicarboxylic acid.

One such method utilizes the reaction of N-protected aspartic anhydrides with the selected amino compound of 10 formula III. Thus compounds of formula III can be reacted directly in inert organic solvents with L-aspartic anhydride having its amino group protected by a formyl, carbobenzloxy, or p-methoxycarbobenzloxy group which is sequentially removed after coupling to give compounds of general formula 15 I. The N-acyl-L-aspartic anhydrides are prepared by reac-ting the corresponding acids with acetic anhydride in amounts of 1.0-1.2 moles per mole of the N-acyl-L-aspartic acid at 0 to 60C in an inert solvent. The N-acyl-L-aspartic anhydrides are reacted with preferably 1 to 2 moles 20 of compounds of formula III in an organic solvent capable of dissolving both and inert to the same. Representative solvents are ethyl acetate, methyl propionate, tetrahydro-furan, dioxane, ethyl ether, N,N-dimethylformamide and benzene. The reaction proceeds smoothly at 0 to 30C. The 25 N-acyl group is removed after coupling by catalytic hydro-genation with palladium on carbon or with HBr or HCl in a conventional manner. U.S. Patent 3,879,373 discloses that this coupling method can also be performed in an aqueous solvent at a temperature of -10 to 50 and at a pH of 4-12.

3s . .
.

~.~85282 1 Compounds of Formula II~ that i9 the amino compounds, can be prepared by art-recognized procedures, 3o .85282 , In any of the previous synthetic methods the desired products are preferably recovered from reaction mixtures by crystallization. Alternatively, normal or reverse-phase chromatography may be utilized as well as liquid/liquid extraction or other means.

The desired compounds of formula I are usually obtained in the free acid form; they may also be recovered as their physiologically acceptable salts, i.e., the corresponding amino salts such as hydrochloride, sulfate, hydrosulfate, nitrate, hydrobromide, hydroiodide, phosphate or hydrophos-phate; or the alkali metal salts such as the sodium, potas-sium, lithium, or the alkaline earth metal salts such as calcium or magnesium, as well as aluminum, zinc and like salts.
Conversion of the present new compounds of formula I
into their physiologically acceptable salts is carried out by conventional means, as for example, bringing the com-pounds of formula I into contact with a mineral acid, an alkali metal hydroxide, an alkali metal oxide or carbonate or an alkaline earth metal hydroxide, oxide, carbonate or other complexed form.

These physiologically acceptable salts can also be utilized as sweetness agents usually having increased solu-bility and stability over their free forms.

It is known to those skilled in the art that the com-pounds of the present invention having asymmetric carbon atoms may exist in racemic or optically active forms. All of these forms are contemplated within the scope of the invention.

- ,:

.
.

1 ~285282 1 The compounds of the present invention have one asymmetric site, which is designated by an asterik(*) in the formula below, and at least one pseudo-asymmetric site which is designated by a double asterik(**):

B'NH - CH -- CONH -- C(A) (A' ) * ** .
Z
y There may also be asymmetric sites in Z and Y depending on the nature of the substituents.
Whenever A is identical to A'~ the compound3 of the present invention have one asymmetric site, designated by the asterik, in the dicarboxylic acid moiety, and may haveothers depending upon the nature of Z
and Y. Although both the D and L forms are possible, the preferred compounds are those in which the dicarboxylic acid group is inthe L-configuration. Whenever the groups A' and A are different, the carbon atom~ designated by the double asteriks become asymmetric center~
and the compounds of the present invention will contain at least two asymmetric centers. Regardles~, the configuration around each of the asymmetric sites, whenever present, may exist in either the D and L
forms, and all possible stereoisomers are contemplated to be within the scope of the present invention.
Since the aspartyl group is in the L-configuration, whenever an asymmetric center is present at any of the other possible asymmetric sites, the compounds 8~
.

1 of the present invention are diastereomers, which can be separated, if desired, by art-recognized techniques, as, for examples, chromatography. However, mixtures of at least two stereoisomers will also exhibit sweetness properties and are useful as sweeteners.
The following examples further illustrate - the invention.

3o ~.~8S28X

N-alpha-L-aspartYl-2-amino-4-(2,2,4,4-tetramethvl-thietan-3-yl)trans-3-butene A. Homoloqation of diethvlmalonate To a stirring mixture of NaH (12.49 g, 0.52 mol) in 300 mls of anhydrou THF at 0C. under argon, was added 50 9 (0.312 mol) of diethyl malonate.
The reaction stirred for 0.5 hours as hydrogen gas evolved. Once the complete formation of the anion 10 was certain, 25.17 mls (23.09 9, 0.312 mol) of ethyl formate was added dropwise over a period of ten minutes.
The solution was allowed to stir for two hours and was then quenched by the addition of 200 mls of saturated ammonium chloride. The reaction was extracted three times with 100 ml portions of diethyl ether. The organic portions were combined and washed once with saturated ~odium bicarbonate, once with water, and dried over anhydrous magnesium sulfate. The ethereal Aolution was filtered, concentrated, and purified by flash chromatography to yield the desired product as a colorle3s oil.
B. 2,4-DihYdro-2,4-dimethvl-3-formvlPentane The homologated diester (25 9, 0.136 mol) was placed in a two-necked flask along with 135 mls of diethyl ether at -78C. under argon. 5 Equivalents of methylmagnesium bromide (226 mls, 0.63 mol) was then added slowly to the well-mixed solution. The reaction stirred for two hours when 200 mls of saturated ammonium chloride was introduced to the reaction.
After fifteen minutes of agitation, the mixture was extracted three times with 100 ml portions of diethyl ~.~8~;i282 1 ether. The ethereal extracts were combined and washed once with 100 ml~ of saturated sodium bicarbonate, and once with 100 mls of water. The organic layers were combined, dcied over magnesium sulfate, filtered, and concentrated. Chromotographic purification re~ulted in the i301ation of the de~ired di-carbinol.
C. 2,4-Dibromo-2,4-dimethyl-3-formylpentane To a magnetically ~tirred solution of N-bromosuccinimide (28.48 9, 0.16 mol) in THF (500 ml~), a solution of triphenylphosphine (41.92 9, 0.16 mol) in THF was added dropwi~e; and exothermic reaction resulted with a white solid separating.
To this suspension, a solution of dicarbinol (12 9, 0.08 mol) in THF, was added and stirring wa~ continued until the solid went into solution. The mixture was concentrated in vacuo and the residue wa~ treated with water and ether. The organic layer was separated, wa~hed with water, dried over magnesium sulfate, and concentrated to afford the desired product.
D. 1-(2~4-Dibromo-2~4-dimethyl-3-pentyl)buten-l-ol To a magnetically stirred solution of 6.05 9 (0.05 mol) oS l-bromopropene in 200 mls of Trapp mixture (THF/diethylether/pentane 4:1:1) was cooled under argon to -120C. After ten minute~ of stirring, tert-butyl lithium (59 mls, 0.001 mol) wa~ added to the mixture. The product from the previous reaction (12.87 9, 0.045 mol) was added and stirring continued for fifteen minutes at -78C. and for twenty minutes at room temperature. The mixture was quenched by 3 pouring into a separatory funnel containing 0.01 mol of acetic acid, saturated ~odium chloride and methylene chloride. The organic layer was qeparated, dried over magnesium sulfate, and concentrated to afford the de~ired allylic alcohol.

'8~i282 . .

1 E 1-(2,4-Dibeomo-2,4-dimethyl-3-pentyl3-2-butenyl-.

1-(2,2,2-trichloroacetimidate) A flame dried three necked flask containing a solution of 15 9 (0.046 mol) of 1-(2,4-dibromo-2,4-dimethyl-3-pentyl)-2-buten-1-ol in 45 mls of anhydrous THF
(lM) at 0C. under argon was treated poctionwi~e with a hexane slurry of 0.3~ g (0.0092 mol) of potassium hydride (a 35~ dispersion in mineral oil which had been washed twice with hexane). After stirring for ten minutes, hydrogen evolution ceased. The yellow alkoxide solution was transferred, via a double needle syringe, to a solution of 4.6 mls (0.046 mol) of trichloroacetonitrile in 100 mls of diethyl ether at 0C. under argon. The reaulting mixture darkened quickly and was allowed to stir at 0C. for 2 hours.
The mixture was taken up in 200 mls of 1~ ethanolic hexane and shaken vigorously for two minutes. Dark insoluble material precipitated and was promptly filtered. The filtrate was then concentrated to afford the crude acetimide.
F. 2,2,2-Trichloro-N-[2-methyl-3-butenyl-4-(2,4-dibromo-2,4-dimethYl-3-pentane)-1 A solution of crude imidate (21.6 g, 0.046 mol) in 200 mls of xylene was brought to reflux and monitored by IR. After 3 hours, the isomerization was complete. Concentration in vacuo afforded the crude acetamide.

3o ~85Z82 . .

l G. Preparation of 2-amino-4-(2,2,4,4-tetramethyl thietanyl)-3-butene Sodium metal (2.12 9, 0.092 mol) was dissolved in 50 mls of anhydrous methanol and the mixture wa~
then cooled to 0C. Hydrogen sulfide gas was passed through the mixture until a saturated solution was obtained. Then the crude acetamide (0.046 mol) was added dropwise in methanol while continuing to allow hydrogen sulfide to pas3 through the reaction mixture. After the addition waq complete, the reaction waa stirred for two hours at 0C., allowed to warm to room temperature and stirred overnight. After pouring the mixture into water, it was extracted with die~hyl ether and the extract~ washed with lM HCl and saturated sodium chloride. After drying over magneqium sulfate, filtering and concentration resulted in the product which was used without purification.
H. Preparation of Dipeptide To a three necked flaqk equipped with an overhead stirrer, was added 0.98 g (0.0046 mol) of 2-amino-3-butenyl-4-(1,1,3,3-tetramethylthietane) in 10 mls of water. The solubility of the amine was enhanced by the addition o 4 mls of THF. The flask was cooled to 0C. and 0.80 g (0.0046 mol) of NTA was added in small portions. A pH of ll was recorded prior to the NTA addition and decreased to a range of 8.5-9.5 during the addition. Once the addition was complete, the pH stabilized at 10.5.
After 2 hours of stirring, the mixture waa acidified 3 with concentrated HCl to a pH of 4.5. The mixture was filtered through *Celite, washed through with *Trade Mark 85~82 1 25 ml~ of methanol, and concentrated in vacuo. The crude product wa~ purified by reversed pha~e flash chromotography u~ing 70% methanol-water a~ an eluant to ~fford the de~ired product a3 a white solid.
Using the foregoing procedure, the following productq are obtained from corresponding starting compounds:
N- C~-L-aspartyl-2-amino-2-methyl-4-(2,2,4,4-tetramethyl-thietan-3-yl)-trans-3-butene N- d -L-aspartyl-2-amino-2-methyl-4-(2,4-dimethylthietan-3-yl)-trans-3-butene;
N- c~ -L-aspartyl-2-amino-2-methyl-4-~2,2- dimethylthietan-3-yl)-trans-3-butene;
N- d -L-aspartYl-2-amino-2-methyl-4-(2,2,4-trimethyl-thietan-3-yl)-trans-3-butene;
N-C~ -L-aspartyl-2-amino-2-methyl-4-(2,4,4-trimethyl-thietan-3-yl)-trans-3-butene;
N-C~ -L-aspartyl-2-amino-2-methyl-4-(4,4- dimethylthietan-3-yl)-trans-3-butene;
N- C~ -L-aspartyl-2-amino-2-methyl-4-( ~ , ~ '-diethyl-thietan-3-yl)-trans-3-butene;
N- C~ -L-aspartyl-2-amino-2-methyl-4-( ~ -tertbutyl-thietan-3-yl)-trans-3-butene;
N- c~ -L-aspartyl-2-~mino-4-(2,4-dimethylthietan-3-yl)-trans-3-butene;
N- c~ -L-aspartyl-2-amino-4-(2~2-dimethylthietan-3-yl)-trans-3-butene;
N-CX -L-aspartyl-2-amino-4-(2~2~4-trimethylthietan-3-yl) trans-3-butene;
3 N- C~ -L-aspartyl-2-amino-4-(2,4,4-trimethylthietan-3-yl)-trans-3-butene:
N- c~ -L-aspartyl-2-amino-4-(4,4-dimethylthietan-3-yl)-trans-3-butene;

~.285282 1 N- c~ -L-aspartyl-2-amino-4-( ~ ,~ '-diethylthietan-3-yl)-trans-3-butene;
N- C~ -L-aspartyl-2-amino-4-( ~ -tertbutylthietan-3-yl)-trans-3-butene;
N- c~ -L-aspartyl-2-amino-l-methoxy-4-(2,2,4,4-tetramethyl-thietan-3-yl)-trans-3-butene;
N- ~ -L-aspartyl-2-amino-l-hydroxy-4-(2,2,4,4-tetramethyl-thietan-3-yl)-trans-3-butene;
N- ~ -L-aspartyl-2-aminO-4-(2,2,4,4-tetramethyl-thietan-3-yl)-trans-3-butenoic acid methyl ester;
N- G~ -L-aspartyl-l-amino-l-[2-(2,2,4,4-tetramethyl-thietan-3-yl)-trans-ethenyl]cyclopropane.

,~ .

_~ q _ ~ 2852~2 N-Alpha-L-aspartyl-2-amino-4-(2,2,4,4-tetramethyl-thietan-3-yl)butane Method A
The product from Part F of Example 1 is treated with H2 gas at 45 psi over 5% Pd/C until reduction of the double bond is completed and the product is o~tained from the reaction mixture by filtration and evaporation.
Method B
The crude acetamide from paragraph F, Example 1, was dissolved in 100 mls of ethanol and placed in an ultrasound bath. This vessel was then purged with argon.
Cyclohexadiene (10 equiv.) was added, followed by 0.1 , equivalents of 10~ Pd/C. Ultrasound was commenced for one-half hour. After one half hour the reaction was completed, as indicated by TLC. The solution was filtered through *Celite and concentrated to afford the desired product which was used to prepare the saturated analogue of the product from step H.
The reaction product is then converted to the dipeptide by the procedure of paragraphs 7 and 8 of Example 1.
Using these procedures, the following products are produced from the corresponding unsaturated compound.
N- ~ -L-aspartyl-2-amino-2-methyl-4-(2,2,4,4-tetramethyl-thietan-3-yl)butane;
N- C~ -L-aspartyl-2-amino-2-methyl-4-(2,4-dimethylthietan-3-yl)~utane;
N- c~ -L-aspartyl-2-amino-2-methyl-4-(2,2-diemethylthietan-3-yl)butane;
N- ~ -L-aspartvl-2-amino-2-methyl-4-(2,2,4-trimethyl-thietan-3-Yl)butane;

*Trade ~ark ~.285282 1 N-C~ -L-aspartyl-2-amino-2-methyl-4-(2,4,,4-trimethyl-thietan-3-yl)butane;' N-c~ -L-aspartyl-2-amino-2-methyl-4-(4,4-diemethylthietan-3-yl)butane;
N- c~ -L-aspartyl-2-amino-2-methyl-4-( ~ , ~ '-diethyl-thietan-3-yl)butane;
N- c~ -L-aspartyl-2-amino-2-methyl-4-( ~ -tertbutyl-thietan-3-yl)butane;
N- c~ -L-aspartyl-2-amino-4-.(2,4-dimethylthietan-3-yl)~utane;
N- c~ -L-aspartyl-2-amino-4-(2,2-dimethylthietan-3-yl)butane;

N-CX -L-aspartyl-2-amino-4-(2~2~4-trimethylthietan-3-yl) butane;
N- C~ -L-aspartyl-2-amino-4-(2~4~4-trimethylthietan-3-yl) butane;
N- d -L-aspartyl-2-amino-4-(4~4-dimethylthietan-3-yl) butane;
N- c~ -L-aspartyl-2-amino-4-( ~ ,~ '-diethylthietan-3-yl) butane;
N- 6~ -L-aspartyl-2-amino-4-( ~ -tertbutylthietan-3-yl) .butane;
N- ~ -L-aspartyl-2-amino-1-methoxy-4-(2,2,4,4-tetramethyl-thietan-3-yl) butane;
N- ~ -L-aspartyl-2-amino-1-hydroxy-4-(2,2,4,4-tetramethyl-: thietan-3-yl) butane;
N- ~ -L-aspartyl-2-amino -4-(2,2,4,4-tetramethyl-thietan-3-yl) butanoic acid methyl ester;
N- ~ -L-aspartyl-l-amino-1-[2-(2,2,4,4-tetramethyl-3 thietan-3-yl) -ethyllcyclopropane.

, 35 .~

- ~!1.;28528~

(2,2,4,4-Tetramethyl thietanyl) N-alpha-L-aspartyl-2-amino isobutyrate A.N-Boc-2-amino isobutyric acid 2-amino isobutyric acid was N-~oc protected as described in the literature in 62%
yield. (J. Miss. Acad. Sci. 29, 13, 1984).
B.(2,2,4,4-tetràmethyl thietanyl) N-Boc-2-amino isobutyrate The N-protected amino acid (3.7 9, 18.3 mmol) was dissolved in 1,2-dichloroethane (50 ml) at 0C. under argon. A solution of N,N-dimethylamino pyridine (0.5 equiv.) and 2,2,4,4-tetramethyl thietanyl alcohol (1 equiv.) in 1,2-dichloroethane (10 ml) was added. Lastly, dicyclohexylcarbodiimide (1.1 equiv.) was added as a solid. After five days of stirring at room temperature, the urea waq removed by filtration and the filtrate was hi-vacuum rotary evaporated and then diluted with petroleum ether (50 ml). The solution was clarified again by filtration and the filtrate waq hi-vacuum rotary evaporated to a pa~te. Column chromatography on silica gel with 6:1 petroleum ether/ethyl acetate gave the pure product (4.4 9) in 81~ yield as a white crystalline solid. The structure of the product was confirmed by 300 MHz proton and carbon NMR and ~A3 mass spectrometry.
NMR (CDC13) ~ 1.44 (s,9H), 1.54 (s,6H), 1.55 (d,12H), 5.0 (~,lH).
C.(2,2,4,4-tetramethyl thietanyl)2-Aminoisobutyrate A fresh solution of trimethylsilyl iodide was prepared at 0C. under argon by dissolving 1.47 g of sodium iodide in 20 ml of acetonitrile. Then 1.26 ml of trimethylsilyl chloride wa3 added and ~.28S282 1 and the mixture was stirred until a yellowish color developed over 0.5 hours. The N-~oc protected amino ester from above, 1.14 9, was dissolved in 75 ml of chloroform at 0C. and 13.84 ml of the trimethylsilyl iodide solution was added via syring~e. The reaction was stirred for 2 hours to room temperature and then quenched with 20 ml methanol and hi-vacuum rotary evaporated to a solid. The solid was washed with diethyl ether and characterized to be the ammonium iodide salt of the desired product. Yield: 1.4 g D. (2,2,4,4-tetramethyl thietanyl) N-Alpha-L-aspartyl-2-amino_ isobutyrate The salt from above, 360 mg, was dissolved in 6 ml of water of 0C. and adjusted to pH 9.1 with lN NaOH. 276 mg of N-thiocarboxy-L-aspartic acid anhydride (NTA) was added as a solid slowly while the pH was maintained at 9.0-9.2 with the addition of lN NaOH. Upon completion of addition of the NTA the pH was adjusted as above for 3 hours. When stabilized, 3 ml of methanol was added and the solution was acidified with 2~ hydrochloric - acid to pH 4.5. Ater stirring for 0.5 hours, the mixture was hi-vacuum rotary evaporated to give 680 mg of a solid. The solid was dissolved in methanol and filtered. The filtrate was concentrated to a paste ~nd redissolved in water and applied to an *AG-lX4 acetate form ion exchange column. The product eluted with pure water and was hi-vacuum rotary evaporated to give 125 mg of a white solid. The product was 3 characterized by *FAB mass spectrometry.
Using these procedures, the following products are produced from the corresponding starting compounds.

*Trade Mark ..... .

1 2~35~8~

1 (2,4-dimethylthietanyl) N- c~ -L-aspartyl-2-aminoisobutyrate;
(2,2-dimethylthietanyl) N-d -L-aspartyl-2-aminoisobutyrate;
(2,2,4-trimethylthietanyl) N- C~-L-aspartyl-2-aminoisobutyrate;
(2,4,4-trimethylthietanyl) N- C~ -L-aspartyl-2-aminoisobutyrate;
(4,4-dimethylthietanyl) N-c~ -L-aspartyl-2-aminoisobutyrate;
( ~ , ~ '-diethylthietanyl) N-CX -L-aspartyl-2-aminoisobutyrate;
( ~ -tert-butylthietanyl) N-c~ -L-aspartyl-2-aminoisobutyrate;
(2,2,4,4-tetramethylthietanyl) N- C~-L-aspartyl-2-amino-1-hydroxymethylpropionate;
(2,2,4,4-tetramethylthietanyl) N- C~-L-aspartyl-2-amino-l-methoxymethylpropionate;
(2,2,4,4-tetramethylthietanyl~ N-cX -L-aspartyl-l-amino-cyclopropylcarboxylate.

3o .

~ 8S28~

.

A. 3,3-Diisopropyl methyl acrylate To a dry flask under argon was added diethyl phosphono methyl acetate (1.1 equiv.) in dry benzene.
Sodium hydride (60% dispersion in oil) (1.1 equiv.) was added. The solution was warmed to 60C. until evolution of hydrogen was complete (1.5 h) and a clear mixture was obtained. Diisopropyl ketone (1 equiv.) was dissolved in dry benzene and added to the 60C. solution from above so as to maintain a gentle reflux. After 1 hour the solution was evaporated to a paste and the residue distilled at reduced pressure to afford the desired product.
3. Gamma, gamma-dibromo diisopropyl methyl acrylate ~5 The product from above was treated with N-bromo succinimide (2.1 equiv.) in dry carbon tetra-chloride at 60C. and when thin layer chromatography indicated no remaining starting material the solution was filtered and rotary evaporated. The oil was carried onto the next step without purification.
C. Gamma, gamma-dibromo diisoprop~l methyl acrylate The unsaturated ester from above was dissolved in a 1:1 mixture of water and dimethylformamide.
The solution was treated with chromium sulfate.
(A. Zurquiyah and C.E. Castro, Org. Syn. 49, 98, 1969). Aqueous ammonium sulfate workup and ether extraction followed by distillation gave the desired product.

3o 3..285~

.
1 D. Gamma, gamma-dibromo diisopropyl acetic acid The ester from above in chloroform at 0C.
was treated with the required amount of reagent prepared as described below. A fresh solution of trimethylsilyl iodide was prepared at 0C. under argon by dissolving 1.47 9 of sodium iodide in 20 ml of acetonitrile.
Then 1.26 ml of trimethylsilyl chloride was added and the mixture was stirred until a yellowish color developed over 0.5 hours. Workup was done with methanol and high vacuum rotary evaporation to give the crude product which was purified by filtration through neutral alumina with ether.
E. 2,2,4,4-Tetramethyl thietan~l acetic acid Sodium metal (1 mol) was dis~olved in dry methanol (500 ml) at 10C. and hydrogen sulfide ga~
was passed through the mixture until it was saturated.
The dibromo acid from above (0.33 mol) wa~ added dropwise in methanol while continuing to allow hydrogen sulfide to pass into the solution. After 2 hours at the cold temperature the reaction was stirred at room temperature overnight. After pouring into water and ether extraction the aqueous layer was acidified with dilute acetic acid and re-extracted with ether. After drying with magnesium sulfate and rotary evaporation, cooling produced a solid.
Thi~ material was washed with a minimum of petroleum ether to give a low melting solid.

3o ~85~

1 F.~ Anhydride Formation 2,2,4,4-tetramethyl thietanyl acetic acid (1 equiv.) was dissolved in dichloromethane at room temperature. Dicyclohexylcarbodiimide (0.5 equiv.) was added and the contentq of the flask stirred for 3 days. The urea was removed by filtration and the fi~trate was evaporated. The residue waA taken up in petroleum ether and refiltered. The clarified filtrate was evaporated and distilled at O.S mm Hg.
The product anhydride was characterized by IR spectroscopy.
G. Acylation - Decarboxylation D-alanine (20 g) was disqolved in dimethyl formamide (400 ml) and treated with chlorotrimethylsilane (26-8 9) and stirred at room temperature (lH) until a homogeneous solution was obtained. Meanwhile, N-alpha-tbutyloxycarbonyl beta-tbutyl L-aspartic acid (58 g) was dissolved in a 1:1 mixture of dimethylformamide and tetrahydrofuran (880 ml), cooled to -15C. and treated with N-methyl morpholine (22.4 ml) and isobutyl chloroformate (26.2 ml). After 10 minutes of activation the precooled solution of D-alanine silyl ester from above wa~ added via double syringe. N-methyl morpholine (22.4 ml) was added again. The reaction was warmed to room temperature slowly and stirred for four hours then acidified to pH 2 with aqueous hydrochloric acid. The product was extracted with chloroform and washed with dilute acid and water several times.
After drying with magnesium sulfate and rotary evaporation a white solid was obtained when crystallized with diethyl ether.

.., ,-: .... .
.

~ ~85,'::8~

,~ .

1 N-soc-L-aspartic acid beta-t-butyl ester alpha-DL-alanine (1 equiv.) was stirred at room temperature under argon with triethylamine (3 equiv.) and N,N-dimethylamino pyridine (0.08 equiv.). The anhydride from F, above (1.5 equiv.) was added and the mixture stirred neat for 3 day~. Aqueous dilute acetic acid wa~ added and the mixture extracted with ethyl acetate. The organic layer was wa~hed with ethyl acetate. The organic layer was washed with water and dilute sodium 10 hydrogen carbonate. Drying with magne~ium sulfate followed by rotary evaporation gave a semi-solid.
Chromatography on silica gel with 2:1 petroleum ether/
ethyl acetate a~forded the product, N-soc-L-aspartic acid beta- t-butyl ester alpha-DL-2-amino 4-(2,2,4,4-tetramethyl)thietanyl-3-butanone.
Similarly, using the appropriate starting --terials, the following compounds were prepared:

N-Boc-L-aspartic acid- ~-t-butyl ester- c~-DL-2-amino-4-(2,4-dimethylthietanyl)-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- c~ -DL-2-amino-4-(2,2-dimethylthietanyl)-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- ~ -DL-2-amino-4-(2,2,4-trimethylthietanyl)-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- ~ -DL-2-amino-4-(2,4,4-trimethylthietanyl)-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- ~ -DL-2-amino-4-(4,4-dimethylthietanyl)-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- d -DL-2-amino-4-( ~ , ~ '-diethylthietanyl)-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- d -DL-2-amino-4-( ~ -t-butylthietanyl)-3-butanone;

....

52~2 , .

1 N-Boc-L-aspartic acid- ~ -t-butyl ester-c~ -DL-2-amino-2-methyl-4-(2~2~4~4-tetramethyl)thietanyl-3-butanone;
N-Boc-L-aspartic acid- ~ -t-butyl ester- 6~ -DL-2-amino-1-methoxy-4-(2,2,4,4-tetramethyl)thietanyl-3-butanone;
5 N-Boc-L-aspartic acid- ~ -t-butyl ester-C~ -(DL)-2-amino-l-hydroxy-4-(2,2,4,4-tetramethylthietanyl)-3-butanone;
N-80c-L-aspartic acid- ~ -t-butyl ester- c~-(DL)-2-amino-4-(2,2,4,4-tetramethylthietanyl)-3-oxybutanoic acid methyl ester;
N-Boc-L-aspartic acid- ~ -t-butyl ester- C~-(DL)-l-amino-10 1-1(1-oxo-2-(2,2,4,4-tetramethylthietanyl)ethyl]cyclopropane.

3o ~.28528~

EXAMpLE S
N-alpha-L-aspartyl-DL-2-amino 4-(2,2,4,4-tetramethyl thietanyl) 3-butanol The amido ketone from Paragraph G~ EX.4, was dissolved in 95~ ethanol at 0C. Cerium trichloride (hydrate, 2 equiv.) was added and followed by the addition of sodium borohydride (2 equiv.). The milky solution was stirred for one hour to room temperature and then poured into sufficient water and ethyl acetate to break the emulsion. The organic layer was dried and rotary evaporated to give a white solid which was recrystallized with ether/petroleum ether. The pure product was characterized by FAB mass spectrometry.
The protected dipeptide was treated with 2.1 equiv. of trimethylsilyl iodide as described in Part D. The crude reaction mixture was eYaporated to a paste and chromatographed on C18 reversed phase silica with methanol and water. It was found that severe insolubility in methanol of the C2 L-diastereomers continually precipitated them out of solution. Thus, isolation of the pure sweet dipeptide was an enrichment of the C2 D-diastereomer~.
Using these procedures, the following products are produced from the corresponding precursors.
N- o~-L-aspartyl-DL-2-amino-4-(2,4-dimethylthietanyl)-3-butanol;
; N- C~-L-aspartyl-DL-2-amino-4-(2,2-dimethylthietanyl)-3-butanol;
N- c~ -L-aspartyl-DL-2-amino-4-(2,2,4-trimethylthietanyl)-3 3-butanol;
N- Cl-L-aspartyl-DL-2-amino-4-(2,4,4-trimethylthietanyl)-3-butanol;

:1.28528~

1 N- d -L-aspartyl-DL-2-aminO-4-(4,4-dimethylthietanyl)-3-butanol;
N- c~ -L-aspartyl-DL-2-amino-4-( ~ , ~ '-diethylthietan 3-butanol;
N- ~ -L-aspartyl-DL-2-amino-4-( ~ -t-butylthietanyl)-3-butanol;
N- 6~ -L-aspartyl-DL-2-amino-2-methyl-4-(2~2~4~4-tetra methylthietanyl)-3-butanol;
N- ~ -L-aspartyl-DL-2-amino-1-hydroxy-4-(2,2,4,4-tetra-10 methylthietanyl)-3-butanol.
N- ~ -L-aspartyl-DL-2-amino-1-methoxy-4-(2,2,4,4-tetra-méthylthietanyl)-3-butanol;
N- ~ -L-aspartyl-DL-2-amino-4-(2,2,4,4-tetra-methylthietanyl)-3- hydroxybutanoic acid methvl ester;
N-c~ -L-aspartyl-l-amino-l-~l-hydroxy-2-(2,4,4,4-tetra-methylthietanyl)ethyl]cyclopropane.

: 2 3o ~ 35 :' ~ . ~ .

~.2sæs~

, 1All thin layer ~TLC) separationa were done with Analtech silica (GF) plates and Analtech reverse - phase bonded plates. The preparative chromatography was performed on slurry packed flash columns employing J.T. Baker 40 um flash silica. Reversed phase C18 silica from J.T. Baker was used for de-blocked dipeptide purification.

,;

3o .'

Claims (50)

1. A compound of the formula:
wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or car-balkoxy wherein the alkoxy group contains 1-3 carbon atoms;
A' is hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms;
Z is -CH2CH2-; -CH=CH;
; -CH(OH)-CH2; -CH2O or ; and Y is thietanyl or alkyl-substituted thietanyl containing up to a total of 8 carbon atoms;
B' is H or an amino protecting group with the proviso that when Z is , B' is not H;
and food acceptable salts thereof.

2. The compound according to claim 1 wherein Z is a tetramethylthietanyl.

3. The compound according to claim 1 wherein Z is a trimethylthietanyl.

4. The compound according to claim 1 wherein Z is a dimethylthietanyl.

5. The compound according to claim 1 wherein Z is 2,2,4,4-tetramethylthietan-3-yl.

6. The compound according to claim 1 wherein Z is 2,4-dimethylthietan-3-yl.

7. The compound according to claim 1 wherein Z is 2,2-dimethylthietan-3-yl.

8. The compound according to claim 1 wherein the amino protecting group is COCF3, COCCl3 and CONAr-X, wherein Ar is aryl, X is NO2, CN, COOR", COR", SO2R", halo, carboxy, SO3H, CO3R", SO2NR"R", SO2NH R", SO2NH2, CONR"R", CONHR", CONH2, SOR", , OR", OSO2R", OCH3, CH2OR", CH(OR")2, COCF3, CF3, CH2CF3, CCl3, CtF2t+1, and the like;
wherein each R" is the same or different and is C1-C12 alkyl and t is an integer from 1-6.

9. The compound according to claim 1 which is N-?-L-aspartyl-2-amino-4-(2,2,4,4-tetramethylthietan-3-yl)trans-3-butene.

10. The compound according to claim 1 which is N-?-L-aspartyl-2-amino-4-(2,2,4,4-tetramethylthietan-3-yl)butane.

11. The compound according to claim 1 which is (2,2,4,4-tetramethylthietanyl) N-?-L-aspartyl-2-aminoisobutyrate.

12. The compound according to claim 1 which is N-Boc-L-aspartic acid-A-t- butyl ester-?-DL-2-amino-4-(2,2,4,4-tetramethyl)thietanyl-3-butanone.

13. The compound according to claim 1 which is N-?-L-aspartyl-DL-2-amino-4-(2,2,4,4-tetramethylthi-etanyl)-3-butanol.

14. A compound of the formula:
wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or car-balkoxy wherein the alkoxy group contains 1-3 carbon atoms;
A' is hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms;
Z is -CH=CH-; and Y is thietanyl or alkyl-substituted thietanyl containing up to a total of 8 carbon atoms;
and food acceptable salts thereof.

15. The compound according to claim 14 wherein Z is a tetramethylthietanyl.

16. The compound according to claim 14 wherein Z is a trimethylthietanyl.

17. The compound according to claim 14 wherein Z is a dimethylthietanyl.

18. The compound according to claim 14 wherein Z is 2,2,4,4-tetramethylthietan-3-yl.

19. The compound according to claim 14 wherein Z is 2,4-dimethylthietan-3-yl.

20. The compound according to claim 14 wherein Z is 2,2-dimethylthietan-3-yl.

21. A compound of the formula:
wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or carbalkoxy wherein the alkoxy group contains 1-3 carbon atoms;

A ' is hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms;
Z is Y is thietanyl or alkyl-substituted thietanyl containing up to a total of 8 carbon atoms;
B' is an amine protecting group;
and food acceptable salts thereof.

22. The compound according to claim 21 wherein Z is a tetramethylthietanyl.

23. The compound according to claim 21 wherein Z is a trimethylthietanyl.

24. The compound according to claim 21 wherein Z is a dimethylthietanyl.

25. The compound according to claim 21 wherein Z is 2,2,4,4-tetramethylthietan-3-yl.

26. The compound according to claim 21 wherein Z is 2,4-dimethylthietan-3-yl.

27. The compound according to claim 21 wherein Z is 2,2-dimethylthietan-3-yl.

28. The compound according to claim 21 wherein B' is COCF3, COCCl3 and CONAr-X, wherein Ar is aryl, X is NO2, CN, COOR", COR", SO2R", halo, carboxy, SO3H, SO3R", SO2NR"R", SO2NH R", SO2NH2, CONR"R", CONHR", CONH2, SOR", OR", OSO2R", OCF3, CH2OR", CH(OR")2, COCF3, CF3CH2CF3, CCl3, CtF2t+1, and the like;
wherein each R" is the same or different and is C1-C12 alkyl and t is an integer from 1-6.

29. A compound of the formula:
wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or car-balkoxy wherein the alkoxy group contains 1-3 carbon atoms;
A' is hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms;
Z is Y is thietanyl or alkyl-substituted thietanyl con-taining up to a total of 8 carbon atoms;
and food acceptable salts thereof.

30. The compound according to claim 29 wherein Z is a tetramethylthietanyl.

31. The compound according to claim 29 wherein Z is a triemethylthietanyl.

32. The compound according to claim 29 wherein Z is a dimethylthietanyl.

33. The compound according to claim 29 wherein Z is 2,2,4,4-tetramethylthietan-3-yl.

34. The compound according to claim 29 wherein Z is 2,4-dimethylthietan-3-yl.

35. The compound according to claim 29 wherein Z is 2,2-dimethylthietan-3-yl.

36. A compound of formula:

wherein A is hydrogen, alkyl containing 1-3 carbon atoms, hydroxyalkyl containing 1-3 carbon atoms, alkoxymethyl wherein the alkoxy contains 1-3 carbon atoms or car-balkoxy wherein the alkoxy group contains 1-3 carbon atoms;
A' is hydrogen or alkyl containing 1-3 carbon atoms;
A and A' taken together with the carbon atom to which they are attached form cycloalkyl containing 3-4 carbon atoms;
Z is -C-O-O
Y is thietanyl or alkyl-substituted thiethanyl containing up to a total of 8 carbon atoms;
and food acceptable salts thereof.

37. The compound according to claim 36 wherein Z is a tetramethylthietanyl.

38. The compound according to claim 36 wherein Z is a trimethylthietanyl.

39. The compound according to claim 36 wherein Z is a dimethylthietanyl.

40. The compound according to claim 36 wherein Z is 2,2,4,4-tetramethylthietan-3-yl.

41. The compound according to claim 36 wherein Z is 2,4-dimethylthietan-3-yl.

42. The compound according to claim 36 wherein Z is 2,2-dimethylthietan-3-yl.

43. An edible composition comprising a sweetening effective amount of a compound according to claim 1, together with an edible substance.

44. An edible composition according to claim 43 which further comprises a food acceptable carrier.

45. An edible composition according to claim 43 which is a beverage.

46. An edible composition according to claim 43 which is a gelatin dessert.

47. An edible composition according to claim 43 which is a milk-based composition.

48. An edible composition according to claim 43 which further comprises an additional sweetener.

49. An edible composition according to claim 48 wherein the additional sweetener is sucrose, fructose, corn syrup solids, dextrose, xylitol, sorbitol, man-nitol, acetometa-aminobenzoic acid, meta-hydroxybenzoic acid, cyclamate, chlorosucrose, or dihydrochalcone, hydrogenated glucose syrup, aspartame or other dipep-tides, glycyrrhizin or stevioside or mixtures thereof.

50. Use of the compound of any one of claims 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41 or 42 as a sweetening agent.