CA1145671A - Glycol-iodine composition and method of preparation - Google Patents

Abstract

TITLE

GLYCOL-IODINE COMPOSITION AND METHOD OF PREPARATION

INVENTOR
Samuel RUBEN

ABSTRACT
A composition is disclosed which comprises a glycol component including ethylene glycol, its polymers and mixtures thereof, and iodine, which composition is characterized by the absence of a spectroscopically identifiable complex between the glycol and iodine, and the absence of free iodine in solu-tion. More particularly, the glycol may comprise ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol and mixtures thereof, and the iodine content of the composition may vary and may include as mauch as 5 to 10%
titratable iodine content. In a preferred embodiment, the composition of the present invention contains ethylene glycol and up to about 5% iodine, and preferably may contain from about .05% to 5% iodine. This composition is prepared by a process which comprises adding to the glycol component the appropriate amount of iodine under agitation, heating the resultant mix-ture to about 180°C, followed by cooling the resultant solu-tion to room temperature The composition of the present in-vention may be prepared in a wide variety of formulations, and possesses therapeutic, environmental and industrial clean-ing applications.

Description

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BACKGROUND OF THE INVENTION
The present invention relates to organic iodine containing compositions, and more specifically to such com-positions as release active iodine therefrom.
In general, the preparation of iodine containin9 compositions is well documented. For example, U.S. Patent ; No. 1,675,554 to Hoopman discloses a medicinal composition containing iodine in concert with a variety of glycerine.
The composi-tion is not prepared in the manner of my invention and, moreover, lacks all of the ingredients employed therein.
Further, U.S. Patent No. 1,596,651 to Bryant discloses an emollient composition including iodine, phenol, thymol and petrolatum oil and is accordingly distinguishable in the scope of ingredients and the emphasis on the employment of thymol to reduce the iodine content of the composition.
U.S. Patent No. 1,719,523 to Stevens discloses a composition iodine and an alcohol. Patentee obtains a composition con-taining ethylene iodide which is clearly distinguishable from the composition of the present invention.
U.S. Patent No. 1,580,400 To Bomlllarito relates to a composition comprising elementary iodine, potassium iodide, guaicol, distilled water and glycerine, for use in treatment of goiter by injection into the patient. In this composition, the glycerine serves as a solvent and is pur-ported to reduce irritation incident to the injection pro-cess. The elementary iodine in Bommarito is stated to be ; the pure iodine found in the crystalline state, and the presence of this iodine, along with potassium iodide dis-tinguishes Bommarito from the present invention.

-2-U.S. Patent No. 1,767,667 to Gray relates to a germioidal compound which employs the ingredients of zinc iodide, iodine in the resublimed form, menthol, glycerine, alcohol and water. Gray prepares his composition by a com-bination of the ingredients under low heat maintained below - 40C. By its definition, the composition of Gray is clearly distinguishable from that of the present invention.
U.S. Patent No. 1,013,913 to Wemer relates to an iodine containing composition specifically comprised of iodine in mixture with glycerite of tannic acid. Wemer alleges that the crystalline iodine is in fact dissolved in the tannin and glycerine without changing the character-istics or properties thereof. Wemer is silent, however, with respect to the method of preparing his solution and, more importantly, does not suggest the application of heat in the manner taught by the present invention. Moreover, the composition of Wemer is distinguishable from that of the present invention in the employment of the tannic acid component.
Finally, U.S. Patent No. 1,896,171 to Harry re-lates to an iodine containing composition which employs iodine in mixture with glycerine and/or glycerol and tannic acid. A review of the Harry disclosure suygests that the tannic acid component is contemplated as a material ingred-ident (see Page 2, line 126 - Page 3,line 6). Further, and more importantly, Harry discloses and claims that his compo-sition contains free iodine which is in clear distinction to the teachings of the present invention.
In addition to the foregoing patent references, Applicant is aware oi literature citations pertinent on the

-3-~ '7 subject of the present invention. Specifically, an article by Osol and Pines relating to the solubility of iodine in glycol-water solutions is reported in the Journal of the American Pharmaceutical Association, at ~olume 41, Page 634, wherein the authors review the solubility of iodine and various glycols, including ethylene and propylene glycols, along with the effect of adding water to solutions of iodine in the solvents. All of the investigations, however, were conducted at 25C and, as such, do not comtemplate the preparation of Applicant's composition. Moreover, the re-lationship proposed by the authors to exist between the iodine and the respective glycols, comprising the existence of a complex analogous to triiodide ion, is distinguishable from the composition of the present invention, particularly as brought out hereinafter.
The most recent investigation of the interaction of iodine with various glycols is presented in a l97l Ph.D.
dissertation by G.D. Faile of Auburn University. The author conducted extensive ultraviolet spectroscopic investiga-tions of various glycol-iodine systems, including that of ethylene glycol-iodine, all prepared at 25C and speculated that a complex relationship develops that the author re-ferred to as charge-transfer complexes. Specifically, ultra-violet spectroscopic analysis of ethylene glycol-iodine dis-closes a maximum absorption at 231 nanometers, which the author indicated is clearly indicative of the complex above noted. Faile, like the references noted earlier, however, does not appreciate the present invention and its specific method of preparation which employs a much higher temperature upon mixture, and for reasons to be elaborated hereinafter, ; ~ -4-7~ I
further highlights by his work the unexpected properties of the composition of the present invention.
SUMMARY OF THE INVENTION
In accordance with the present invention, a com- ¦
`position is disclosed which comprises a glycol component ~' .
including ethylene glycol, its polymers and mixtures there-of, and iodine, which composition is characterized by the absence of a spectroscopically identifiable complex between the glycol and iodine, and the absence of free iodine in solution. More particularly, the glycol may comprise ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol and mixtures thereof, and the iodine content of the composition may vary and may include as much as 5 to 10%
;titratable iodine content. In a preferred embodiment, the composition of the present invention contains ethylene glycol and up to about 5% iodine, and preferably may contain from about .05% to 5% iodine.
` The compos;tion of the present invention may be prepared by a process which comprises adding to the glycol component the appropriate amount of iodine under agitation, heating the resulting mixture to about 180C, followed by cooling the resulting solution to room temperature. In a preferred embodiment the method of the present invention is conducted in the total absence of all moisture, under sub-stantially anhydrous conditions, and is thereafter main-tained in the anhydrous state.
The composition of the present invention possesses a variety of utilities including employment as a medicinal aid, disinfectant and the like having utility in therapeutic, environmental and industrial maintenance applications, as 7~
well as any applications requiring the presence and activity of iodine. The composition operates in response to moisture to release free iodine in molecular form, while retaining the iodine in the absence of moisture to release free iodine in molecular form, while retaining the iodine in ; the absence of moisture in a state whereby the application of an iodine indicator to the composition yields negative results. Further, spectrographic analysis of the present composition, and in particular, observation of the ultra-violet spectrum yields the absence of a reaction indicating an iodine-glycol complex at the wavelength at which such complex is known to exhibit an absorption peak. Further, the composition of the present invention may be embodied in a wide variety of preparations including the placement within various dispersants, vehicles, emollients and the like depending upon the nature of the end utility desired.
Accordingly, it is a principal object of the present invention to provide an iodine containing composi-tion which retains iodine in stable solution and releases said iodine in noncrystalline, molecular form upon contact with an aqueous environment.
It is a further object of the present invention to provide an iodine containing composition as aforesaid that facilitates the rapid release of rnolecular iodine and is not toxic in topical application to the human body.
It is a yet further object of the present inven-tion to provide an iodine containing composition as afore-said which exhibits favorably increased shelf stability.
It is a yet further object of the present inven-tion to provide an iodine containing composition as aforesaid ' .
--6--., '7~

which exhibits unexpectedly improved antimicrobial activity.
It is a yet further object of the present inven-tion to provide a method of preparing an iodine containing composition which yields a composition retaining iodine in stable solution.
Other objects and advantages will become apparent to those skilled in the art from a review of the ensuing de-scription which proceeds with reference to the following accompanying figures.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 comprises an ultraviolet spectrograph of the mixture of ethylene glycol and iodine in accordance with the prior art.
Figure 2 is an ultraviolet spectrograph obtained from analysis of the composition of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
In accordance with the present invention the foregoing objects and advantages are readily attained.
The present invention comprises a composition comprising a glycol component comprising ethylene glycol, its polymers and mixtures thereof, and iodine, which compo-sition is characterized by the absence of a spectroscopically identifiable complex between the glycol and iodine, and the absence of free iodine therein. More particularly, the glycol component of the present invention may comprise a compound selected from the group consisting of ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, and mixtures thereof, and in a preferred embodiment, the glycol component comprises ethylene glycol.
The present composition is distinguishable from ~ '7~
the prior art by its negative response to ultraviolet spectrographic analysis respecting the presence of a glycol-iodine complex as had been reported in the prior art in the doctoral thesis by Faile, discussed earlier. Further, when prepared in accordance with the method of the present in-vention, the present compositions respond negatively to tests for free iodine in solution, suggesting that the iodine is maintained in some form of complex other than the con-; ventional alcohol-iodine charge transfer complex suggested in the literature. This form of composition appears to ensure the substantially complete retention of iodine in solution over extended periods of time, thereby conferring a favorably extended shelf life. Further, in addition to the ability of the present composition to effectively sequester iodine in a distinctive complex relationship, the composi-` tion is capable of releasing non-crystalline, molecular iodine from solution upon immediate contact with an aqueous environment, including moisture. Finally, the present com-position has been found to possess improved microbiocidal activity with reduced causticity to the body which is accordingly desirable in a wide variety of medicinal applica-tions.
As noted earlier, the composition of the present invention appears to comprise a unique mixture which may be characterized as a reaction product, in that the compounds contained therein interact in some fashion after their com-bination in accordance with the present method. Specifically, the iodine component appears unavailable in the free state and is, moreover, not seen to complex in the conventional manner with the alcohol moities of the glycol. Earlier dis-cussion focusing on the thesis of Faile indicated that the .

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author experimented with various iodine-alcohol systems, in particular the system comprising ethylene glycol and iodine, to determine the interaction believed to exist be-tween alcohol substituents and iodine. The author proceeded to conduct tests comprising ultraviolet spectral analysis and determined that certain absorption bands observed re-flected the existence of a complex between the alcohol and iodine, referred to by the author as a charge-transfer complex.
Applicant has undertaken to conduct comparative testing to determine whether the composition of the present invention possesses a structure of the type characterized by Faile, or whether some different structure exists. The comparative experiments are set forth below.
EXPERIMENT I
A composition compr;s;ng ethylene glycol dried in contact w;th magnesium sulfate and subsequently distilled was then placed in an aqueous solution to exhibit up to 1.0 mole fraction concentration of alcohol. One hundred mill;-liters of this solution was then made 10 4 M in HC104 to suppress triiodide formation. Three milliliters of the resulting stock solution were then placed in the sample cell of an ultraviolet spectrophotometer to observe the ultra-violet spectrum thereof. To the sample cell containing the stock solution, three crystals of resublimed iodine were added and stirred. The original stock solution was located in a reference compartment and a spectrum was then run. The foregoing procedure was followed in accordance with the ex-periments conducted by Faile, supra., as set forth on pages 11-15. The resulting spectrum, presented in Figure 1, indi-cated that, as observed by the author, an absorption peakoccurs in the area of 231 nanometers which suggests that a charge-transfer complex exists between the alcohol substituents ~ S~i7~
of the ethylene glycol and the iodine.
EXPERIMENT II
By comparison the composition of the present invention was prepared by reacting the ethylene glycol and iodine components in the absence of water in a commercial ` reaction vessel by heating the components to a temperature of about 160C, followed by cooling the resulting solution to room temperature while maintaining the solution in an air and moisture exclusive environment. A sample of the solution was then placed in the ultraviolet spectrophotometer and, as a reference, the ethylene glycol prepared in accordance with Faile was employed. The spectrum observed shows the absence of absorption in the areas noted by the author, and, particularly, indicated a marked increase in transmittance in the area of 230-240 nanometers. As can be seen in Figure 2, two spectra were run simultaneously, each comprising pro-duction preparations of the composition of the present inven-tion containing 3~ iodine, as well as the comparative ethyl-ene.glycol reference sample. From the above, it is apparent that the exact structure of the composition of the present invention indeed varies from that both known and expected in the prior art as represented by Faile.
In addition to the structural distinction dis-cussed above, the foregoing tests further established that no free iodine is present in the solutions prepared in accord-ance with the present invention. Particularly, as noted by Faile on page 67 thereof, the appearance of an absorption band at 203 nanometers was indicative of the existence of free iodine in a glycol solution. Referring again to Figure 2, however, no such absorption appears to exist, as the transmittance of the sample is seen to increase at that .
:

.

' 7:1 wavelength. The above tests are therefore believed to establish both the absence of a complex existing between the alcohol substituent of the glycol component and iodine, and the absence of free iodine from the solution comprising the composition of the present invention.
As indicated earlier, the composition of the present invention may be prepared in a wide variety of iodine concentrations to suit the end utility for which the composition is intended. Thus, the iodine content of the com-position of the present invention may vary up to the saturationpoint of iodine in the glycol component, and may, for example, extend up to 5-10% iodine. More particularly, the composi-tions containing on the order of about 5~ iodine may be pre-pared for use in certain medicinal and industrial applications, and in a further embodiment such compositions may contain from .05-5% iodine.
In accordance with the present invention, a method of preparation is also disclosed which is bel;eved to contribute to the distinctive propert;es of the present composition. More particularly, an important element of the method of the present invention is that the glycol and iodine components are heated with agitation to a tempera-ture on the order of 180C. Once this temperature is reached, the completed product is removed from the heat source and allowed to cool to room temperature. In a preferred embodi-ment, the glycol component is provided in substantially anhydrous condition and may be prepared, for example, by ex-posure to magnesium sulfate to remove all moisture there-from. The glycol is then retained in substantially anhydrous conditions, either by maintenance in an inert atmosphere or vacuum, the appropriate quantity of iodine in crystal ~s~

- form is added thereto and the resulting mixture agitated to dissolve the iodine crystals. The resulting solution is then brought to the aforementioned temperature while main-tained urlder anhydrous conditions, and subsequently cooled to room temperature to form the completed reaction product.
An important feature of the method of the present invention is that the composition is heated to a temperature of approximately 180C. Applicant notes that, whereas com-positions containing higher alcohols such as glycerol and the like form an unhydrolyzable compound by reaction within this temperature range, the composition of the invention is readily hydrolyzable upon contact with an aqueous environment to yield free iod;ne in molecular form. More particularly, the method of the present invention may be practiced at a temper-ature ranging from about 140C to about 180C, and in a pre-ferred embodiment, a reaction temperature of 160C may be employed.
A further feature of the method comprises the employment of anhydrous reaction conditions to produce a composition substantially free from water. The employment of anhydrous reaction conditions has been unexpectedly found to add greater stability to the resulting composition, as it assists in the retention of iodine in combination with the glycol such that iodine indicator tests showed negative results and the absence of free iodine from solution. Though the establishment and maintenace of anhydrous reaction con-ditions is preferred, it is not mandatory, as the composition of the present invention may be prepared under conditions permitting exposure to air and consequent moisture while ~ LS~71 nonetheless resulting in a composition possessing all of the aforementioned properties. Accordingly, the invention is not to be construed as limited to the ~mployment of anhydrous conditions, but such conditions are to be con-sidered as representative of a best mode of practicing the invention.
As indicated earlier, the composition of the present invention may be emobodied in a wide variety of applications and, accordingly, may be formulated with various additives, dispersants, carriers and the like to facilitate its employment therein. Specifically, the present composi-tion has been found to possess substantial antibacterial and antimicrobial efficacy and, in the instance where a top-ical preparation for use with the human body is contemplated, the composition may be mixed with an appropriate dispersant such as propylene glycol for application in liquid form.
Preferably, such a mixture may, for example, be prepared by combining one part by volume of the composition of the present invention with three parts by volume of propylene glycol.
In the instance where an ointment or salve is desired, the composition of the present invention may be admixed with a suitable quantity of a bodying agent or car-rier such as mannitol or a polyethylene glycol having a higher molecular weight on the order of 6000, in proportions which may vary between the components to provide a prepara-tion varying in consistency from that of a cream to that of a thick paste. Further, other materials may be employ-ed which serve to provide appropriate media for presentation . ~
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of the present composition depending upon the uti1ity de-sired. Thus, for example, the composition of the present invention may also be employed as a liquid additive in such environments as fish breeding waters, and in water purification and dental sterilization, as well as hard sur-face cleaning applications. In each instance, the composi-tion may be employed individually or in combination with appropriate additives.
The scope of the present invention is illustra-ted with reference to the examples, which proceed below.
EXAMPLE I
Two grams of iodine crystals were added to 100 grams of ethylene glycol under agitation in a S00 milliliter beaker at room temperature. The ethylene glycol-iodine mix-ture was then heated to a temperature of approximately 160C
with continued agitation, and upon reaching that temperature was immediately removed from the heat source and permitted to cool to room temperature. The beaker was maintained tightly covered and no further agitation was applied during the cooling period.

9.0 grams of iodine wre placed in a loosely covered 500 milliliter beaker at room temperature, and ~50 grams of ethylene glycol were then added thereto under agitation. The ethylene glycol-iodine mixture was then heated to a temperature approaching 160C with continued agitation. Upon reaching 160C, the beaker containing the composition was immediately removed from the heat source and the solution was allowed to cool slowly to room temperature .

'7:1 under tight cover and without further-ag-itation.
One part by volume of the above composition was then mixed at room temperature with three parts by volume of propylene ~lycol. The resulting mixture was then tested for free iodine by dipping of starch indicator paper into a por-tion thereof. The starch indicator paper sho~ed no reaction indicating the absence of free iodine from the solution.

This example illustrates the preparation of the composition of the present invention wherein the glycol component comprises diethylene glycol. Accordingly, 5.37 grams of ;odine was placed in a loosely covered 250 milli-liter Erlenmeyer flask, and 263.0 grams of diethylene gly-col was then added thereto under agitation. The resulting mixture, which possessed an iodine content of approximately 2% by weight, was then heated to a temperature approaching 160C with continued agitation. Upon reaching 160C the flask was removed from the heat source and permitted to cool slowly to room temperature under tight cover and without fur-ther agitation. The resulting composition was then titratedwith .lN Na2S203 standard solution and found to contain 1.35 available iodine.
The foregoing composition compares favorably with a comparable composition prepared with monoethylene glycol which, when titrated, yielded 1.30% available iodine.
Further, in all other respects, the composition of Example 3 responded in like manner to those of Examples 1 and 2.

The composition of the present invention was .

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prepared wherein the glycol component comprised polyethylene glycol. Specifically, 3 grams of iodine were placed in a loosely covered 500 milliliter container at room temperature, and 97 grams of polyethylene glycol having a molecular weight ` of 400 was then added thereto under agitation. The glycol-iodine mixture was then heated to a temperature approaching 160C with continued agitation, after which the container was then removed from the heat source and the solution per-mitted to cool slowly to room temperature under tight cover and without further agitation. The above product was then titrated with .lN Na2S203 standard solution and was found to contain approximately 1.915% available iodine. The compo-sition as prepared responded negatively to tests for available iodine, however, upon mixture with water, responded posi-tively to iodine indicator testing.
The composition of the present invention is char-acterized by its ability to release molecular iodine on con-tact with an aqueous medium and, accordingly, is useful in instances where oxidation or the localized effect of iodine on organic compounds is desirable. Moreover, the composi-tion possesses antimicrobial specificity which renders it useful as an antiseptic and disinfectant in industrial uses.
The composition is likewise nontoxic to living tissue and is accordingly acceptable for use as a topically applied medicinal preparation.
Certain tests were conducted to establish the foregoing utilities, which tests are presented below.

The composition of the present invention was .

prepared ~ith a free iodine titration of 0.3% and was diluted in trypticase soy broth (TSB) in a series of concentrations ranging from 0.0003- 0.075% free I2. The respective growth media prepared were then inoculated with the following organ-isms which were then grown at 35C for 16-18 hours; Staphylo-_ccus aureus ATCC #6538, Staphylococcus aureus - Gregg strain, Staphylococcus albus 914-4-lA, Pseudomonas cepacia 153, Sarcina lutea ATCC #9341RR, Escherichia coli ATCC #10536, Pseudomonas aeruginosa 568-18-lA and Enterobacter cloacae 603-1-lB. Inoculation was accomplished by adding 0.05 milliliters (1 drop) of the overnight broth culture to 10 milliliters of antimicrobial agent in TSB. Transfer tubes, used to determine cidal endpoints for the titrated compounds, were prepared by adding the neutralizers, Tween 8 ~ (0.5~), lecithin (0.07%), and sodium thiosulfate (1%), to TSB.
The inoculated tubes containing the dilutions of test compounds were incubated for 48 hours at 35~C;
the presence of growth (+) for each tube was recorded. from the tube showing no growth (-), 1.0 milliliters of solution was transferred to 10 milliliters of neutralization broth, and the tubes containing the neutralization broth were then incubated for 48 hours at 35C. The absence of growth in these tubes determined the cidal endpoint for each sample.
The results of these tests are set forth in Table 3.

~T
,.., ,~ o l l l l l l l l u o ' 1~-- I

.~ z a rl _ ___ _ .~ ~ a ~ o _.__ ___ . __ _ ~ .
.~ ~ C . ~ o ~I ~I l l + + ~

, < 1 U L O _ _ __ __ ;
~ O U O l .1 .+ ~ l ~ + +
. , C _ _ __._ __, .. ~ ____, _ .__ _ ___ ,' ~ ~ . 1~ O . + ~ +- ~ ~ + ~

C ..... 1_ _ .___._._,.. .... ~ .~ ... ~__.. ,.. _ ._. ,,.. ~.,_._ _,._.____ _ _ . O ~ . .1. ~ ~ ~ ~t t-~,. ~ G :.~ 3~'_ - __.,.,- _ U C V~ .___~ .. .~nO., -~ ~ , '~ .--_ . _ _=
v. u ~¦ v _ u < v~ r: :~ ~ ~ r. v~ ~ u ~, v, v ~ ~ v v r u ~ ~ c v r ,~ -- _ o ~ v~ C u u _ 3 _ O C/ ~ ~ , O ~ ~J O v~ o~ U _ _ O C ~ r.. 1:
C ~ ~ ~ _ ~ v. _ _ I E c _ ~ c~ _ O ~ El -- ~ r u _ I t~
: ~ 1 ~. ~ ~~ O u ~:: ~, u O t~ _ O ~ ~ O
~_ ~ ~ ~_; -- r, ~: ~ _ ~ ~ u ~ u ~ _ L. ~ o ~ c~ C
C ~ ~: ~J ~: U L~ 1- V ~_ U U ~ v U ~
~J C ~ L ~ 0 C < V, < V) C V~ C
~; i _.. __.. U~ ~, =~ __~ h~ ----~" l+~
' . . .

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From the tests tabulated above, it was deterrnined that the composition of the present invention required a con-centration of 0.03~ free iodine to prove cidal for gram-posi-tive bacteria, and a concentration of 0.05~ free iodine to be cidal for gram negative bacteria. Thus, the cidal activ-ity of the composition of the present invention is clearly in evidence.

The composition of the present invention was tested for dermal toxicity in accordance with the Hazardous Substances Labeling Act Regulations, Part 191, Chapter 1, Title 21, Code of Federal Regulations, Paragraph 191.10 by application of a dosage level of 2.2 grams/kilogram con-ta;ned on a gauze pad to the bare skin of six rabbit subjects, and the retention of the pads in position for measured inter-vals of 24 and 72 hours. The results of the tests were that no deaths were caused by the composition, and the composi-tion was thereby determined to be dermally nontoxic.

Further toxicity studies were conducted com-prising eye irritation testing. Accordingly, 0.1 milli-liter samples of the composition tested in Experiment 4 `~ were applied to the eyes of albino rabb;ts in accordance with the method set forth in the Hazardous Substances Labeling Act Regulations, Part 191, Chapter 1, Title 21, Code of Federal Regulations, Paragraph 191.12. Specifically, the composition was applied to one of the rabbits' eyes with the other eye retained as a control. The eyes are .

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subsequently examined and the rate of ocular reaction noted daily for a total of 7 days. Six rabbit subjects were em-ployed for the tests and the results thereof are set forth le ~

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TABLE 5 - EYE IR~lTATION TESTING

Fin._in~s: Antiseptic Compound .YS
12 ~ 4 ~ 6 7 `~ Rabbit ~ 1 ; I. Cornea A. O~acity ~O O O
. Are a OO O O O O O
x 5 II. Iris :~. A. Values OO O O O O O
x5 : III. Conjunctivae : -;: A. i`c~iness O O O O O O O
: . B . Cller,los is O O O O O O O
.' 1 C. Discllargc O O O O u O O
. (A~+C) x2 Total O O ~
.. Rabbit ~ 2 I . Corn e a A; Ol~acity OO O O O O O
B. Area OO O O O
AxBxS
lI. Iris A. Valucs OO O O O O O
.~ x5 III. Conjunctivae ~ A. F;edncss OO O O O O O
`~ 13 . Chcr;~os i s - O O O O O O O
C. Dischar,~e OO O O O O O
(AtI3~C) x2 Total d~~ ~ O ~ ~O
., l~abbit ~ 3 , . . .
. I. Corne~
A. Oj~aci ty O O O O O O O
. L~. Area O O O O O O
:~ AxBx5 :, .
``. II. Iris ,~ A. ~'a~ ues O O O
:,: xS
:
: III. Conjunctivae .~. A. P.edness OO O O . O O O
:~ Ii . C;lenos is O O O O . O O O
C. Disc]~arge OO O O O O O

(A~B~C) x2 Tot al O O O O O O O

',~
. .

, . .
' ~ _ ~:;
, .

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TABLE 5 (cont.) ~in~ings: Antisep~ic Compound ~AYS
2 3 4 ~ 6 7 Rabblt ~ 4 I. Cornea A. O~acity O O O O O
B. Area .o O O O O O
x S
II. Iris A. Values O O O O o O O
xS
III. Conjunctivae A. i~e~ness O O O O O O O
- B. Cller.losis O O O O O O O
C. Disc~lar~c O O O O ~ O O
(A~+C) x2 Total 0 0 0 0 0 Rabbit ~ S
I. Corne 2 A. Ol)acity O O O O O O O
B. Area O o O O O O O
Ax~xS
II. Iris A. Valucs O O O O D O O
. x5 III. Conjunctivae A. r;edncss O O O O ¢ O O
. Chcr.osis O O O O O O
C. ilischar~c O O O O O O O
(A+~+C) x2 Total O ~ O O ~ -b O
Ra~bit ~6 I. Cornca A. O~city O o O O 0 0 0 B~ Arca O O O O O O
AxBx5 .
II. Iris A. Va~ues O O O O O .0 0 x5 III. Conjunctivae A. P~edness O O O O O 0 0 . Cncnosis O o O O. O O O
C. . Discllarge O O O O O O O
(A~B~C) xZ
J otal O ~ - -7~

From the above tests, it is apparent that the composition of the present invention produced no irritation in rabbit eyes and as such may be considered non-irritating.
The foregoing data suggests that the composition of the present invention possesses utility in medicinal and general industrial situations where bacteriocidal properties are desirable. Naturally, the composition as indicated earlier is capable of employment in applications of varying utility in addition to those specifically illustrated above, and the scope of the invention is accordingly not limited thereto.
This invention may be embodied in other forms or carried out in other ways without departing from the spirit or essential characteristics thereof. The present ; invention is therefore to be considered as in all respects illustrative and not restrictive, the scope of the inven-tion being indicated by the appended claims, and all changes which come within the meaning and range of equivalency are intended to be embraced thereir.

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Claims (25)

WHAT IS CLAIMED IS:

1. A method for preparing an iodine containing composition having no free iodine present therein which com-prises mixing a quantity of a glycol component selected from the group consisting of ethylene glycol its polymers and mix-tures thereof with a substantially smaller quantity of iodine heating said mixture to a temperature ranging up to about 180°C
and thereafter cooling said mixture to room temperature.

2. The method of claim 1 wherein said tempera-ture ranges from about 140°C to about 180°C.

3. The method of claim 1 wherein said tempera-ture ranges up to about 160°C.

4. The method of claim 1 wherein said glycol component and said iodine are agitated during mixing and heat-ing.

5. The method of claim 1 wherein said glycol component, said iodine and said mixture are maintained in sub-stantially anhydrous conditions.

6. The method of claim 1 wherein said glycol component is selected from the group consisting of ethylene glycol, diethylene glycol triethylene glycol polyethylene glycol and mixtures thereof.

7. The method of claim 1 wherein said glycol component comprises ethylene glycol.

8. The method of claim 1 wherein said iodine and said glycol component are present in amounts sufficient to prepare said mixture to possess an available iodine content of up to about 10% titratable I2.

9. The method of claim 8 wherein said iodine content may range up to about 5%.

10. The method of claim 9 wherein said iodine content may range from about .05 to about 5%.

11. A new composition made according to claim 1 comprising the reaction product formed from heating a glycol component selected from the group consisting of ethylene glycol, its polymers and mixtures thereof, and iodine to a temperature of approximately 180°C.

12. The composition of claim 11 wherein said tem-perature ranges from about 140°C to about 180°C.

13. The composition of claim 11 wherein said tem-perature ranges up to about 160°C.

14. The composition of claim 11 wherein said glycol component is selected from the group consisting of ethylene glycol, diethylene glycol, triethylene glycol, poly-ethylene glycol and mixtures thereof.

15. The composition of claim 11 wherein said gly-col component comprises ethylene glycol.

16. The composition of claim 11 wherein said io-dine is present in an amount ranging up to about 10% titratable I2.

17. The composition of claim 11 wherein said re-action product is prepared and maintained ?nder substantially anhydrous conditions.

18. The composition of claim 16 wherein said iodine is present in an amount ranging up to about 5% titrat-able I2.

19. The composition of claim 18 wherein said iodine content ranges from about 0.05 to about 5%.

20. The composition of claim 19 wherein said iodine content is approximately 1.5%.

21. The composition of claim 11 further includ-ing an additive selected from the group consisting of dispers-ants, carriers, bodying agents, perfumes, emollients, and mix-tures thereof.

22. The composition of claim 21 wherein said additive comprises propylene glycol.

23. The composition of claim 22 wherein said propylene glycol is combined with said composition in the ratio 1 part of said composition to 3 parts of said propylene glycol.

24. The composition of claim 21 wherein said additive comprises mannitol.

25. The composition of claim 21 wherein said additive comprises polyethylene glycol having a molecular weight of about 6000.
(M&Co.File 163-3-1)