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BRPI1012922A2 - polipeptídeo de b-glicosidase variante, vetor de expressão, célula hospedeira, método de produção de polipeptídeo de b-glicosidase variante, composição enzimática, método de conversão de um substrato de biomassa em açucar fermentável e método de produção de álcool - Google Patents

polipeptídeo de b-glicosidase variante, vetor de expressão, célula hospedeira, método de produção de polipeptídeo de b-glicosidase variante, composição enzimática, método de conversão de um substrato de biomassa em açucar fermentável e método de produção de álcool

Info

Publication number
BRPI1012922A2
BRPI1012922A2 BRPI1012922A BRPI1012922A BRPI1012922A2 BR PI1012922 A2 BRPI1012922 A2 BR PI1012922A2 BR PI1012922 A BRPI1012922 A BR PI1012922A BR PI1012922 A BRPI1012922 A BR PI1012922A BR PI1012922 A2 BRPI1012922 A2 BR PI1012922A2
Authority
BR
Brazil
Prior art keywords
glycosidase
production method
variant
polypeptide
converting
Prior art date
Application number
BRPI1012922A
Other languages
English (en)
Inventor
Louis Clark
Sally Rhiannon Postlethwaite
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of BRPI1012922A2 publication Critical patent/BRPI1012922A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445Beta-glucosidase (3.2.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021Beta-glucosidase (3.2.1.21)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
BRPI1012922A 2009-06-16 2010-06-16 polipeptídeo de b-glicosidase variante, vetor de expressão, célula hospedeira, método de produção de polipeptídeo de b-glicosidase variante, composição enzimática, método de conversão de um substrato de biomassa em açucar fermentável e método de produção de álcool BRPI1012922A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18756509P 2009-06-16 2009-06-16
US21802009P 2009-06-17 2009-06-17
PCT/US2010/038879 WO2010148128A1 (en) 2009-06-16 2010-06-16 Beta-glucosidase variant enzymes and related polynucleotides

Publications (1)

Publication Number Publication Date
BRPI1012922A2 true BRPI1012922A2 (pt) 2016-10-11

Family

ID=43306753

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1012922A BRPI1012922A2 (pt) 2009-06-16 2010-06-16 polipeptídeo de b-glicosidase variante, vetor de expressão, célula hospedeira, método de produção de polipeptídeo de b-glicosidase variante, composição enzimática, método de conversão de um substrato de biomassa em açucar fermentável e método de produção de álcool

Country Status (5)

Country Link
US (3) US8357523B2 (pt)
EP (1) EP2443139B1 (pt)
BR (1) BRPI1012922A2 (pt)
CA (1) CA2762616C (pt)
WO (1) WO2010148128A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8916366B2 (en) * 2009-11-20 2014-12-23 Codexis, Inc. Multi-cellulase enzyme compositions for hydrolysis of cellulosic biomass
EP2576775A1 (en) * 2010-06-07 2013-04-10 Yeda Research and Development Co. Ltd. Modified cellulases with enhanced thermostability
EP2606131B1 (en) 2010-08-20 2017-03-08 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
CN103429738A (zh) * 2011-03-24 2013-12-04 旭硝子株式会社 裂殖酵母属酵母的转化体、该转化体的制造方法、β-葡糖苷酶的制造方法及纤维素的降解方法
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
CN117126823B (zh) * 2023-09-01 2024-03-29 华南理工大学 一种酮还原酶突变体及其应用
CN118165960B (zh) * 2024-03-25 2025-03-25 南京大学 一种内切β-1,4-葡聚糖酶突变体及其基因、载体和制备方法
CN118910014B (zh) * 2024-09-03 2025-04-25 广州青囊生物科技有限公司 一种生产稀有人参皂苷CK的β-葡萄糖苷酶突变体及其应用

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DE3009104C2 (de) 1980-03-10 1990-10-04 Odenwald-Chemie GmbH, 6901 Schönau Verwendung eines Epoxydharzes zur Herstellung von feuerhemmenden Schichten oder Überzügen und Verfahren zur Herstellung derselben
CA1338400C (en) 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
US5426039A (en) 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
HUP9701446A1 (hu) 1997-08-27 1999-05-28 Phylaxia-Pharma Gyógyszer-, Oltóanyag és Agrobiológiai Készítményeket Gyártó és Forgalmazó Rt. Eljárás a talaj mikroorganizmus populációjának előnyös kialakítására
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
NZ519043A (en) 1999-11-19 2006-12-22 Yissum Res Dev Co Nucleic acid comprising a gene derived from Aspergillus niger that encodes a polypeptide that has a beta-glucosidase catalytic activity
WO2001075767A2 (en) 2000-03-30 2001-10-11 Maxygen, Inc. In silico cross-over site selection
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US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
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Also Published As

Publication number Publication date
EP2443139A1 (en) 2012-04-25
WO2010148128A1 (en) 2010-12-23
EP2443139B1 (en) 2016-08-10
CA2762616A1 (en) 2010-12-23
US8357523B2 (en) 2013-01-22
CA2762616C (en) 2015-08-18
EP2443139A4 (en) 2013-02-27
US8715975B2 (en) 2014-05-06
US20140004586A1 (en) 2014-01-02
US20100317059A1 (en) 2010-12-16
US20130095530A1 (en) 2013-04-18
US8530201B2 (en) 2013-09-10

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE A 10A ANUIDADE.

B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]