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BRPI0918730A2 - Plantas tolerantes a herbicida - Google Patents

Plantas tolerantes a herbicida

Info

Publication number
BRPI0918730A2
BRPI0918730A2 BRPI0918730-8A BRPI0918730A BRPI0918730A2 BR PI0918730 A2 BRPI0918730 A2 BR PI0918730A2 BR PI0918730 A BRPI0918730 A BR PI0918730A BR PI0918730 A2 BRPI0918730 A2 BR PI0918730A2
Authority
BR
Brazil
Prior art keywords
tolerant plants
herbicide tolerant
herbicide
plants
tolerant
Prior art date
Application number
BRPI0918730-8A
Other languages
English (en)
Inventor
Timothy Robert Hawkes
Paul Richard Drayton
Richard Dale
Original Assignee
Syngenta Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Ltd filed Critical Syngenta Ltd
Publication of BRPI0918730A2 publication Critical patent/BRPI0918730A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/581,2-Diazines; Hydrogenated 1,2-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Catching Or Destruction (AREA)
BRPI0918730-8A 2008-09-15 2009-09-14 Plantas tolerantes a herbicida BRPI0918730A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0816880.9A GB0816880D0 (en) 2008-09-15 2008-09-15 Improvements in or relating to organic compounds
PCT/GB2009/002188 WO2010029311A2 (en) 2008-09-15 2009-09-14 Herbicide tolerant plants

Publications (1)

Publication Number Publication Date
BRPI0918730A2 true BRPI0918730A2 (pt) 2015-08-25

Family

ID=39930198

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0918730-8A BRPI0918730A2 (pt) 2008-09-15 2009-09-14 Plantas tolerantes a herbicida

Country Status (14)

Country Link
US (1) US20110173718A1 (pt)
EP (2) EP2848125A1 (pt)
JP (1) JP5770631B2 (pt)
CN (1) CN102159718A (pt)
AR (1) AR075547A1 (pt)
AU (1) AU2009290688B2 (pt)
BR (1) BRPI0918730A2 (pt)
CA (1) CA2735476A1 (pt)
EA (1) EA201100481A1 (pt)
ES (1) ES2509897T3 (pt)
GB (1) GB0816880D0 (pt)
UA (1) UA107184C2 (pt)
WO (1) WO2010029311A2 (pt)
ZA (1) ZA201101559B (pt)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2350074B1 (de) 2008-10-29 2013-03-06 Basf Se Substituierte pyridine mit herbizider wirkung
US9347046B2 (en) 2009-01-22 2016-05-24 Syngenta Participations Ag Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
WO2010139658A1 (de) * 2009-06-05 2010-12-09 Basf Se Substituierte pyridopyrazine mit herbizider wirkung
US8575068B2 (en) 2010-03-23 2013-11-05 Basf Se Pyrazinothiazines having herbicidal action
BR112012023761A2 (pt) 2010-03-23 2015-10-06 Basf Se piridina substituída de fórmula i, composto de fórmula i, composição e método para controle de vegetação indesejada
CN102906096A (zh) 2010-03-23 2013-01-30 巴斯夫欧洲公司 具有除草作用的吡啶类
BR112012023938A2 (pt) 2010-03-23 2015-09-15 Basf Se piridina substituída da fórmula i, composto da fórmula i, composição e método para controlar vegetação indesejada
WO2011117211A1 (en) 2010-03-23 2011-09-29 Basf Se Substituted pyridazines having herbicidal action
AR081526A1 (es) * 2010-03-23 2012-10-03 Basf Se Piridazinas sustituidas que tienen accion herbicida
AU2011254317A1 (en) * 2010-05-04 2012-11-22 Basf Se Plants having increased tolerance to herbicides
CA2805941A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
WO2012028580A1 (de) 2010-09-01 2012-03-08 Bayer Cropscience Ag Herbizid wirksame pyridyl-ketosultame
EP2627183B2 (en) * 2010-10-15 2021-08-25 Bayer CropScience Aktiengesellschaft Use of als inhibitor herbicides for control of unwanted vegetation in als inhibitor herbicide tolerant beta vulgaris plants
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種syht0h2及偵測其之組合物及方法
US8877683B2 (en) * 2010-12-23 2014-11-04 Basf Se Substituted pyridines having herbicidal activity
WO2012168241A1 (en) 2011-06-09 2012-12-13 Basf Se Substituted pyrazines having herbicidal activity
CN103596959A (zh) * 2011-06-09 2014-02-19 巴斯夫欧洲公司 具有除草活性的取代吡啶类
EA201490884A1 (ru) 2011-11-02 2014-09-30 Басф Се Растения, обладающие повышенной толерантностью к гербицидам
WO2013064964A1 (en) * 2011-11-02 2013-05-10 Basf Se Plants having increased tolerance to herbicides
BR112014029531A2 (pt) * 2012-06-01 2017-06-27 Basf Se composto de piridina, composição e método para controlar vegetação indesejada
BR112014031260A2 (pt) 2012-06-15 2019-08-20 Du Pont métodos e composições que envolvem variantes de als com preferência de substrato nativo
EP3683307A3 (en) 2012-09-14 2020-07-29 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
US20140173775A1 (en) * 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
WO2014097085A1 (en) 2012-12-18 2014-06-26 Basf Se Herbicide-metabolizing cytochrome p450 monooxygenases
EP2935593A2 (en) 2012-12-21 2015-10-28 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
US20160040149A1 (en) 2013-03-14 2016-02-11 Pioneer Hi-Bred International Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
AU2014236154A1 (en) 2013-03-14 2015-09-17 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
US20160102317A1 (en) * 2013-04-30 2016-04-14 Basf Se Plants having increased tolerance to herbicides
WO2014177999A2 (en) * 2013-04-30 2014-11-06 Basf Se Plants having increased tolerance to herbicides
EP2992090A4 (en) * 2013-04-30 2017-01-11 Basf Se Plants having increased tolerance to herbicides
CN105358697A (zh) 2013-04-30 2016-02-24 巴斯夫欧洲公司 具有增加的除草剂耐受性的植物
BR112015024506A2 (pt) * 2013-04-30 2017-10-24 Basf Se métodos para controlar vegetação, para identificar uma heteroarila, para identificar uma sequência de nucleotídeos, para produzir uma célula vegetal e para produzir uma planta, ácido nucleico, célula vegetal, plantas e semente
BR112015021600A2 (pt) 2013-05-24 2017-07-18 Basf Se composto de piridina, composição e método para o controle da vegetação indesejável
CN103266117B (zh) * 2013-05-27 2014-10-08 南京农业大学 苯基脲类除草剂N-脱甲基酶基因pudmA及其应用
ES2776730T3 (es) 2013-09-13 2020-07-31 Pioneer Hi Bred Int Proteínas insecticidas y métodos para su uso
EP3117003B1 (en) 2014-03-11 2019-10-30 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
BR112016023853A2 (pt) 2014-04-17 2017-08-15 Basf Se composto, composição, método para controlar a vegetação indesejável e uso de um composto
WO2016062814A1 (en) 2014-10-24 2016-04-28 Basf Se Substituted pyridine compounds having herbicidal activity
CN104962494B (zh) * 2015-06-25 2017-09-22 东北农业大学 一株对除草剂阿特拉津具有良好降解效果的希瓦氏菌
AU2016319093B2 (en) 2015-09-11 2022-11-03 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
GB201604970D0 (en) 2016-03-23 2016-05-04 Syngenta Participations Ag Improvements in or relating to organic compounds
US11624076B2 (en) 2016-04-21 2023-04-11 BASF Agricultural Solutions Seed US LLC TAL-effector mediated herbicide tolerance
CN106259368B (zh) * 2016-08-16 2019-03-22 安徽丰乐农化有限责任公司 一种含异噁唑草酮的玉米田复配除草剂
CN106631941B (zh) * 2016-12-30 2018-09-28 青岛瀚生生物科技股份有限公司 一种2-甲基-3氯苯基甲硫醚的制备方法
US11371056B2 (en) 2017-03-07 2022-06-28 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
BR112019018056A2 (pt) 2017-03-07 2020-08-11 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos
US11279944B2 (en) 2017-10-24 2022-03-22 BASF Agricultural Solutions Seed US LLC Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS
GB201800305D0 (en) 2018-01-09 2018-02-21 Syngenta Participations Ag Herbicidal compounds
GB201910290D0 (en) 2019-07-18 2019-09-04 Syngenta Crop Protection Ag Herbicidal compounds
GB202116307D0 (en) 2021-11-12 2021-12-29 Syngenta Crop Protection Ag Herbicide resistance
CN114365833B (zh) * 2022-01-05 2023-02-21 西南大学 应用高能纳米冲击磨和气流粉碎制备超微板栗粉生鲜面

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2751347B1 (fr) * 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides
ES2400699T3 (es) * 2000-12-07 2013-04-11 Syngenta Limited HIDROXI-FENIL PIRUVATO DIOXIGENASAS (HPPD) derivadas de plantas y resistentes frenta a herbicidas tricetónicos, y plantas transgénicas que contienen DIOXIGENASAS
AU2002362498A1 (en) * 2001-09-27 2003-04-14 Syngenta Participations Ag Herbicidal composition
EP1318192A2 (en) * 2001-12-06 2003-06-11 greenovation Biotech GmbH Prenyltransferase from Arabidopsis
DE10160139A1 (de) * 2001-12-07 2003-06-18 Bayer Cropscience Gmbh Synergistische herbizide Mittel enthaltend bestimmte Herbizide aus der Gruppe der Benzoylcylohexandione
BR0308740A (pt) 2002-03-19 2007-01-09 Monsanto Technology Llc ácidos nucléicos e polipeptìdeos de homogentisado prenil transferase ("hpt"), e empregos destes
WO2004044173A2 (en) * 2002-11-13 2004-05-27 E.I. Du Pont De Nemours And Company Cis-prenyltransferases from the rubber-producing plants russian dandelion (taraxacum kok-saghyz) and sunflower (helianthus annus)
JP2004352657A (ja) * 2003-05-29 2004-12-16 Bayer Cropscience Ag 水田用除草剤組成物
ATE440947T1 (de) * 2003-12-02 2009-09-15 Basf Se 2-methyl-6-solanylbenzochinon-methyltransferase als ziel für herbizide
ATE544861T1 (de) * 2005-08-24 2012-02-15 Pioneer Hi Bred Int Verfahren und zusammensetzungen für den ausdruck eines polynukleotid von interesse
TWI375669B (en) * 2006-03-17 2012-11-01 Sumitomo Chemical Co Pyridazinone compound and use thereof
JP2007320951A (ja) * 2006-06-01 2007-12-13 Sds Biotech Corp 水田用除草剤組成物
GB0614471D0 (en) * 2006-07-20 2006-08-30 Syngenta Ltd Herbicidal Compounds
CA2658225C (en) * 2006-07-26 2014-02-11 Dow Agrosciences Llc Herbicidal compositions
GB0624760D0 (en) * 2006-12-12 2007-01-17 Syngenta Ltd Herbicidal compounds
GB0722472D0 (en) * 2007-11-15 2007-12-27 Syngenta Ltd Herbicidal compounds

Also Published As

Publication number Publication date
EP2326722A2 (en) 2011-06-01
UA107184C2 (uk) 2014-12-10
ES2509897T3 (es) 2014-10-20
AU2009290688B2 (en) 2015-06-04
AU2009290688A1 (en) 2010-03-18
WO2010029311A2 (en) 2010-03-18
CA2735476A1 (en) 2010-03-18
EP2326722B1 (en) 2014-08-06
GB0816880D0 (en) 2008-10-22
WO2010029311A3 (en) 2010-05-27
CN102159718A (zh) 2011-08-17
EA201100481A1 (ru) 2011-10-31
ZA201101559B (en) 2012-03-28
JP5770631B2 (ja) 2015-08-26
AR075547A1 (es) 2011-04-20
EP2848125A1 (en) 2015-03-18
JP2012502624A (ja) 2012-02-02
US20110173718A1 (en) 2011-07-14

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2479 DE 10-07-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.