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BRPI0813342A2 - " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ". - Google Patents

" composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ".

Info

Publication number
BRPI0813342A2
BRPI0813342A2 BRPI0813342-5A2A BRPI0813342A BRPI0813342A2 BR PI0813342 A2 BRPI0813342 A2 BR PI0813342A2 BR PI0813342 A BRPI0813342 A BR PI0813342A BR PI0813342 A2 BRPI0813342 A2 BR PI0813342A2
Authority
BR
Brazil
Prior art keywords
dna polymerase
stabilizing
methods
performing pcr
enhancing
Prior art date
Application number
BRPI0813342-5A2A
Other languages
English (en)
Inventor
Yi Liu
Ryan Westberry
Scott Hamilton
Original Assignee
Ge Healthcare Bio Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences filed Critical Ge Healthcare Bio Sciences
Publication of BRPI0813342A2 publication Critical patent/BRPI0813342A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
BRPI0813342-5A2A 2007-06-13 2008-06-12 " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ". BRPI0813342A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94366707P 2007-06-13 2007-06-13
PCT/EP2008/057404 WO2008152102A1 (en) 2007-06-13 2008-06-12 Polymerase stabilization

Publications (1)

Publication Number Publication Date
BRPI0813342A2 true BRPI0813342A2 (pt) 2014-10-14

Family

ID=39677430

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0813342-5A2A BRPI0813342A2 (pt) 2007-06-13 2008-06-12 " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ".

Country Status (8)

Country Link
US (1) US20100159528A1 (pt)
EP (1) EP2152864A1 (pt)
JP (1) JP2010528669A (pt)
CN (1) CN101679957A (pt)
AU (1) AU2008263931A1 (pt)
BR (1) BRPI0813342A2 (pt)
CA (1) CA2689068A1 (pt)
WO (1) WO2008152102A1 (pt)

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US7846703B2 (en) * 2006-10-02 2010-12-07 Takara Bio Inc. Method for enhancing polymerase activity
US20110250598A1 (en) * 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
ES2692894T3 (es) 2011-06-08 2018-12-05 Life Technologies Corporation Diseño y desarrollo de nuevos detergentes para su uso en sistemas de PCR
EP2761001B1 (en) 2011-09-26 2018-08-01 Qiagen GmbH Rapid method for isolating extracellular nucleic acids
TWI539034B (zh) * 2012-03-02 2016-06-21 羅門哈斯電子材料有限公司 碳黑及金屬之複合物
US8956816B2 (en) 2012-06-05 2015-02-17 Pacific Biosciences Of California, Inc. Methods and compositions for performing analytical operations
US9085761B1 (en) 2012-06-14 2015-07-21 Affymetrix, Inc. Methods and compositions for amplification of nucleic acids
EP2917364B1 (en) * 2012-11-07 2018-01-03 Qiagen GmbH Control for diagnostic assay
EP2934572A4 (en) 2012-12-20 2016-11-23 Biomatrica Inc FORMULATIONS AND METHODS FOR STABILIZING PCR REAGENTS
CN104560946A (zh) * 2013-10-10 2015-04-29 镇江拜因诺生物科技有限公司 甘油糖基甘油酸作为增效剂在pcr中的应用
EP3063129B1 (en) 2013-10-25 2019-04-17 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
JP2015188343A (ja) * 2014-03-27 2015-11-02 セイコーエプソン株式会社 バイオチップ
ES2786373T3 (es) 2014-06-10 2020-10-09 Biomatrica Inc Estabilización de trombocitos a temperaturas ambiente
CN107075544B (zh) 2014-07-22 2021-01-29 生物辐射实验室股份有限公司 与聚合酶联用的缓冲液
EP3885438A1 (en) 2015-06-10 2021-09-29 QIAGEN GmbH Method for isolating extracellular nucleic acids using anion exchange particles
CN113588501B (zh) 2015-12-08 2024-12-17 生物马特里卡公司 降低红细胞沉降速率
US10941445B2 (en) 2017-03-24 2021-03-09 Bio-Rad Laboratories, Inc. Universal hairpin primers

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
US4758580A (en) * 1985-06-26 1988-07-19 Yamanouchi Pharmaceutical Co., Ltd. Inhibiting growth of tumors with certain substituted phenoxy dimethyl alkanoic acids, esters or salts
US5405774A (en) * 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US6127155A (en) * 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
US5242818A (en) * 1988-08-26 1993-09-07 Toyo Boseki Kabushiki Kaisha Method of producing a thermostable DNA polymerase from Thermus thermophilus
US5322785A (en) * 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5948663A (en) * 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
WO1995024499A1 (en) * 1994-03-10 1995-09-14 Gen-Probe Incorporated Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
CN1123328A (zh) * 1994-11-17 1996-05-29 中国科学院上海生物化学研究所 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst)
US5602011A (en) * 1995-01-18 1997-02-11 Pharmacia Biotech Inc. Purified Thermococcus barossii DNA polymerase
US6033859A (en) * 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
US5861295A (en) * 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US5882904A (en) * 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
US6242235B1 (en) 1998-06-24 2001-06-05 Promega Corp. Polymerase stabilization by polyethoxylated amine surfactants
US20030134292A1 (en) * 2001-10-30 2003-07-17 Farchaus Joseph W. Thermostable DNA polymerases and methods of making same
US7972828B2 (en) * 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent

Also Published As

Publication number Publication date
JP2010528669A (ja) 2010-08-26
US20100159528A1 (en) 2010-06-24
WO2008152102A1 (en) 2008-12-18
EP2152864A1 (en) 2010-02-17
CA2689068A1 (en) 2008-12-18
CN101679957A (zh) 2010-03-24
AU2008263931A1 (en) 2008-12-18

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 6A E 7A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]