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BRPI0810317A2 - novos métodos para a recuperação de proteínas de folhas. - Google Patents

novos métodos para a recuperação de proteínas de folhas.

Info

Publication number
BRPI0810317A2
BRPI0810317A2 BRPI0810317A BRPI0810317A BRPI0810317A2 BR PI0810317 A2 BRPI0810317 A2 BR PI0810317A2 BR PI0810317 A BRPI0810317 A BR PI0810317A BR PI0810317 A BRPI0810317 A BR PI0810317A BR PI0810317 A2 BRPI0810317 A2 BR PI0810317A2
Authority
BR
Brazil
Prior art keywords
rubisco
transgenic
protein recovery
crystals
plants
Prior art date
Application number
BRPI0810317A
Other languages
English (en)
Inventor
Fu Hong
Martin Lo Yangming
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Publication of BRPI0810317A2 publication Critical patent/BRPI0810317A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D9/00—Crystallisation
    • B01D9/0059—General arrangements of crystallisation plant, e.g. flow sheets
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medical Informatics (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • General Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

descrito aqui se encontra um processo para purificação e cristalização de cristais de ribulose 1,5-bisfosfato carboxilasejoxigenase, de tamanho grande, tetragonais e bi-piramidal (rubisco), originários de folhas verdes de todas plantas folhosas, quer transgênicos e não-transgênicos. os cristais de rubisco, de pureza excepcional, não apenas facilitam as etapas subseqüentes de reconstituição, tais como filtragem ou secagem, como também melhoram a aparência do produto originário da proteína, aspecto essencial em relação à aceitação do consumidor. o rubisco, como enzima de fotossíntese, pode ser amplamente utilizado na determinação das mudanças ambientais e climáticas e como agente medicinal de exame para finalidades de diagnóstico. além disto, também possui muitas aplicações no âmbito das industrias de alimentação, cosmética, química e outros ramos. estas mesmas técnicas também podem ser úteis na recuperação e purificação de proteínas de outras plantas.
BRPI0810317A 2007-05-16 2008-05-15 novos métodos para a recuperação de proteínas de folhas. BRPI0810317A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93826707P 2007-05-16 2007-05-16
PCT/US2008/006205 WO2008143914A1 (en) 2007-05-16 2008-05-15 Novel methods for recovery of leaf proteins

Publications (1)

Publication Number Publication Date
BRPI0810317A2 true BRPI0810317A2 (pt) 2019-03-19

Family

ID=40122048

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0810317A BRPI0810317A2 (pt) 2007-05-16 2008-05-15 novos métodos para a recuperação de proteínas de folhas.

Country Status (6)

Country Link
US (3) US9321806B2 (pt)
EP (1) EP2160399A4 (pt)
CN (1) CN101687906A (pt)
BR (1) BRPI0810317A2 (pt)
CA (1) CA2681295C (pt)
WO (1) WO2008143914A1 (pt)

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CN102475189A (zh) * 2010-11-23 2012-05-30 贵州大学 一种小型实用牧草、绿肥叶蛋白饲料生产工艺
US9301544B2 (en) 2013-03-14 2016-04-05 R.J. Reynolds Tobacco Company Protein-enriched tobacco-derived composition
US9175052B2 (en) 2013-05-17 2015-11-03 R.J. Reynolds Tobacco Company Tobacco-derived protein compositions
CN104186424B (zh) * 2014-07-15 2017-02-22 中国烟草总公司广东省公司 利用丝光绿蝇幼虫生物处理不适用鲜烟叶同时获得有机肥和生物蛋白的方法
CN104621339A (zh) * 2015-01-23 2015-05-20 沈阳化工大学 一种提取水飞蓟分离蛋白的方法
CN104628013B (zh) * 2015-01-28 2016-06-08 徐明好 一种利用污泥中蛋白质生产碳酸铵的方法
US11612183B2 (en) 2015-12-10 2023-03-28 R.J. Reynolds Tobacco Company Protein-enriched tobacco composition
US20170165252A1 (en) * 2015-12-10 2017-06-15 Niconovum Usa Inc. Protein-enriched therapeutic composition
CN106135633A (zh) * 2016-07-01 2016-11-23 河南工业大学 一种食源性富硒抗氧化肽及其制备方法
MX2019011609A (es) * 2017-03-28 2020-01-30 Dietz Max Metodo de procesamiento economico de desconexion/desprendimiento de componentes de materias primas de origen vegetal, su obtencion y uso.
US10745682B2 (en) 2017-06-14 2020-08-18 R.J. Reynolds Tobacco Company Method of producing RuBisCO protein fibers
US20180362957A1 (en) 2017-06-14 2018-12-20 R. J. Reynolds Tobacco Company RuBisCO Protein-Based Films
US10757964B2 (en) 2017-07-20 2020-09-01 R.J. Reynolds Tobacco Company Purification of tobacco-derived protein compositions
CN111432647A (zh) * 2017-12-05 2020-07-17 帝斯曼知识产权资产管理有限公司 脱色的油菜籽蛋白质分离物
CN108314715A (zh) * 2018-01-15 2018-07-24 东北农业大学 一种鲁梅克斯叶蛋白的提取方法
US11666068B2 (en) 2018-05-30 2023-06-06 Leafpro, Llc Sweet potato leaf protein concentrates and methods for obtaining the same
CN109337811B (zh) * 2018-10-15 2021-10-12 天津医科大学 低共熔溶剂整体柱酶反应器及其制备方法
CN109321540A (zh) * 2018-11-13 2019-02-12 中国烟草总公司郑州烟草研究院 一种提高烟叶提取物中还原酶酶活的提取方法
EP3911167A1 (en) 2019-01-18 2021-11-24 R. J. Reynolds Tobacco Company Plant-derived rubisco protein purification
CA3152560A1 (en) * 2019-04-17 2020-10-22 Virentia Innovation Inc. Compositions comprising fabaceae family plant components, processes of preparation and uses thereof
CN120081893A (zh) * 2019-07-11 2025-06-03 浮萍蛋白食品公司 从植物材料分离高纯度蛋白质制品的工艺及其产品
JP2022547977A (ja) 2019-09-11 2022-11-16 ニコベンチャーズ トレーディング リミテッド タバコを白色化するための代替方法
US12063953B2 (en) 2019-09-11 2024-08-20 Nicoventures Trading Limited Method for whitening tobacco
US11369131B2 (en) 2019-09-13 2022-06-28 Nicoventures Trading Limited Method for whitening tobacco
US11937626B2 (en) 2020-09-04 2024-03-26 Nicoventures Trading Limited Method for whitening tobacco
KR20230110270A (ko) 2020-10-21 2023-07-21 비렌시아 이노베이션 인코포레이티드 콩과 식물 성분을 포함하는 조성물, 이의 제조 공정 및 용도
CN112354261A (zh) * 2020-11-10 2021-02-12 北京金华雅轩健康科技有限公司 一种天然产物有机成分萃取提纯工艺
CN112641101A (zh) * 2020-12-24 2021-04-13 常州市芙丽佳生物科技有限公司 包含橄榄仁提取物的蛋白粉及其制备方法
CN112552371A (zh) * 2020-12-24 2021-03-26 常州市芙丽佳生物科技有限公司 一种基于萃取提纯技术制备橄榄仁提取物的方法
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DK202370631A1 (en) * 2023-12-20 2025-07-08 Univ Aalborg Method for producing a food grade protein product and/or a feed protein product from plant material

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Also Published As

Publication number Publication date
US20100093054A1 (en) 2010-04-15
US9944917B2 (en) 2018-04-17
EP2160399A4 (en) 2010-09-15
CA2681295A1 (en) 2008-11-16
EP2160399A1 (en) 2010-03-10
US20160208236A1 (en) 2016-07-21
CA2681295C (en) 2023-03-28
US20180105804A1 (en) 2018-04-19
US10006019B2 (en) 2018-06-26
CN101687906A (zh) 2010-03-31
WO2008143914A1 (en) 2008-11-27
US9321806B2 (en) 2016-04-26

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2516 DE 26-03-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]