[go: up one dir, main page]

BRPI0814244A2 - Método para obtenção de uma planta geneticamente engenheirada - Google Patents

Método para obtenção de uma planta geneticamente engenheirada

Info

Publication number
BRPI0814244A2
BRPI0814244A2 BRPI0814244-0A2A BRPI0814244A BRPI0814244A2 BR PI0814244 A2 BRPI0814244 A2 BR PI0814244A2 BR PI0814244 A BRPI0814244 A BR PI0814244A BR PI0814244 A2 BRPI0814244 A2 BR PI0814244A2
Authority
BR
Brazil
Prior art keywords
engineed
genetically
plant
obtaining
genetically engineed
Prior art date
Application number
BRPI0814244-0A2A
Other languages
English (en)
Inventor
Francois Eudes
Archana Chugh
Original Assignee
Ca Minister Agriculture & Food
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Minister Agriculture & Food filed Critical Ca Minister Agriculture & Food
Publication of BRPI0814244A2 publication Critical patent/BRPI0814244A2/pt
Publication of BRPI0814244B1 publication Critical patent/BRPI0814244B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0814244A 2007-06-07 2008-06-09 método para obtenção de uma planta geneticamente engenheirada BRPI0814244B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92900607P 2007-06-07 2007-06-07
PCT/CA2008/001112 WO2008148223A1 (en) 2007-06-07 2008-06-09 Nanocarrier based plant transfection and transduction

Publications (2)

Publication Number Publication Date
BRPI0814244A2 true BRPI0814244A2 (pt) 2014-10-21
BRPI0814244B1 BRPI0814244B1 (pt) 2018-10-09

Family

ID=40093127

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0814244A BRPI0814244B1 (pt) 2007-06-07 2008-06-09 método para obtenção de uma planta geneticamente engenheirada

Country Status (11)

Country Link
US (2) US8680366B2 (pt)
EP (1) EP2181193B1 (pt)
JP (1) JP2010532159A (pt)
CN (1) CN101889091B (pt)
AU (1) AU2008258254B2 (pt)
BR (1) BRPI0814244B1 (pt)
CA (1) CA2693525C (pt)
MX (1) MX2009013325A (pt)
RU (1) RU2485180C2 (pt)
WO (1) WO2008148223A1 (pt)
ZA (1) ZA200908635B (pt)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
WO2009063460A2 (en) * 2007-11-13 2009-05-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Methods of modulating production of phenylpropanoid compounds in plants
US8923125B2 (en) * 2008-10-24 2014-12-30 Qualcomm Incorporated Wireless network resource adaptation
JP2011067192A (ja) * 2009-08-25 2011-04-07 Sumitomo Chemical Co Ltd 植物の開花時期を制御する遺伝子、タンパク質およびその利用
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
NZ598419A (en) * 2009-10-16 2013-11-29 Dow Agrosciences Llc Use of dendrimer nanotechnology for delivery of biomolecules into plant cells
ES2641642T3 (es) 2010-03-08 2017-11-10 Monsanto Technology Llc Moléculas de polinucleótido para regulación génica en plantas
EP2643459B1 (en) 2010-11-24 2017-09-27 Takara Bio USA, Inc. Inducible expression system transcription modulators comprising a distributed protein transduction domain and methods for using the same
CA2820739A1 (en) * 2010-12-08 2012-06-14 National Research Council Of Canada Microspore transformation methodology by macroinjection
US20120190107A1 (en) * 2011-01-26 2012-07-26 Dwayne Bisgrove Enhanced protein transduction
CA2831144A1 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
KR20140020965A (ko) * 2011-03-23 2014-02-19 다우 아그로사이언시즈 엘엘씨 식물에서의 영상화 및 전달 적용에 적합한 양자점-캐리어 펩티드 접합체
CA2842722C (en) * 2011-08-04 2020-06-09 Francois Eudes Organelle targeting nanocarriers
UY34328A (es) 2011-09-13 2013-04-30 Monsanto Technology Llc ?composiciones y métodos para controlar malezas comprendiendo un polinucleótido y agente de transferencia, y que modulan protoporfirinógeno ix oxidasa?.
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
AR087862A1 (es) * 2011-09-13 2014-04-23 Monsanto Technology Llc Metodos y composiciones para el control de malezas
BR112014005958A2 (pt) * 2011-09-13 2020-10-13 Monsanto Technology Llc métodos e composições químicas agrícolas para controle de planta, método de redução de expressão de um gene accase em uma planta, cassete de expressão microbiana, método para fazer um polinucleotídeo, método de identificação de polinucleotídeos úteis na modulação de expressão do gene accase e composição herbicida
MX350774B (es) * 2011-09-13 2017-09-15 Monsanto Technology Llc Métodos y composiciones para el control de malezas.
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
CA2848669A1 (en) * 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control targeting epsps
BR112014005795A2 (pt) * 2011-09-13 2020-12-08 Monsanto Technology Llc métodos de controle de plantas, de redução da expressão de um gene de hppd de uma planta, de preparação de um nucleotídeo, e de identificação de polinucleotídeos úteis na modulação da expressão do gene de hppd no tratamento externo de uma planta, composições e cassete de expressão microbiana
WO2013040057A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
CN107739737A (zh) * 2011-09-13 2018-02-27 孟山都技术公司 用于杂草控制的方法和组合物
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
CN102441177A (zh) * 2011-12-05 2012-05-09 中国人民解放军军事医学科学院放射与辐射医学研究所 用于siRNA传递的纳米泡载体
RU2478711C1 (ru) * 2011-12-19 2013-04-10 Открытое акционерное общество "Институт стволовых клеток человека" Способ повышения эффективности вирусной трансдукции
JP6074771B2 (ja) * 2012-02-08 2017-02-08 国立大学法人 奈良先端科学技術大学院大学 フロリゲンの導入方法
JP6337771B2 (ja) * 2012-02-27 2018-06-06 国立研究開発法人理化学研究所 植物細胞に核酸を導入する方法
CN104471067B (zh) 2012-05-07 2020-08-14 桑格摩生物治疗股份有限公司 用于核酸酶介导的转基因靶向整合的方法和组合物
IN2014MN02404A (pt) * 2012-05-24 2015-08-21 Seeds Ltd Ab
EP2861737B1 (en) 2012-06-19 2019-04-17 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
KR20150060969A (ko) 2012-10-05 2015-06-03 다우 아그로사이언시즈 엘엘씨 저항성 가을 거염벌레 곤충의 관리를 위한 조합에서의 Cry1Ea의 사용
BR112015008706A2 (pt) 2012-10-18 2018-02-06 Monsanto Technology Llc métodos e composições para controle de praga de plantas
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
EA032406B1 (ru) * 2013-01-01 2019-05-31 Эй.Би. СИДЗ ЛТД. СПОСОБЫ ВВЕДЕНИЯ дсРНК В СЕМЕНА РАСТЕНИЙ ДЛЯ МОДУЛЯЦИИ ЭКСПРЕССИИ ГЕНОВ
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
CA2905104A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Control of lolium species by topical application of herbicidal composition comprising dsrna
WO2014164761A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US12331303B2 (en) 2013-03-15 2025-06-17 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
PL3527068T3 (pl) 2013-03-15 2022-10-10 Cibus Us Llc Sposoby i kompozycje do zwiększania skuteczności ukierunkowanej modyfikacji genu z użyciem naprawy genu, w której pośredniczy oligonukleotyd
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
CN104073519A (zh) * 2013-03-27 2014-10-01 北京市农林科学院 细胞穿透肽介导外源基因转化芸薹属植物小孢子的方法
AU2014248208C1 (en) 2013-04-05 2023-04-27 Corteva Agriscience Llc Methods and compositions for integration of an exogenous sequence within the genome of plants
AU2014279694B2 (en) * 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
PL3030663T3 (pl) 2013-07-19 2020-04-30 Monsanto Technology Llc Kompozycje i sposoby kontroli leptinotarsa
US20160208272A1 (en) 2013-08-22 2016-07-21 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
GB201315321D0 (en) 2013-08-28 2013-10-09 Koninklijke Nederlandse Akademie Van Wetenschappen Transduction Buffer
UY35817A (es) 2013-11-04 2015-05-29 Us Agriculture ?composiciones y métodos para controlar infestaciones de plagas y parásitos de los artrópodos?.
KR102269374B1 (ko) 2013-11-04 2021-06-28 코르테바 애그리사이언스 엘엘씨 최적 대두 유전자좌
US10273493B2 (en) 2013-11-04 2019-04-30 Dow Agrosciences Llc Optimal maize loci
EP3066110B1 (en) 2013-11-04 2021-12-29 Corteva Agriscience LLC Optimal maize loci
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
AR099092A1 (es) 2014-01-15 2016-06-29 Monsanto Technology Llc Métodos y composiciones para el control de malezas utilizando polinucleótidos epsps
WO2015133652A1 (ja) * 2014-03-06 2015-09-11 国立研究開発法人理化学研究所 植物の形質転換法
EA201691581A1 (ru) 2014-03-14 2017-02-28 Кибус Юс Ллс Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов
EP3420809A1 (en) 2014-04-01 2019-01-02 Monsanto Technology LLC Compositions and methods for controlling insect pests
US10988764B2 (en) 2014-06-23 2021-04-27 Monsanto Technology Llc Compositions and methods for regulating gene expression via RNA interference
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
BR112017000482A2 (pt) 2014-07-11 2017-11-07 Du Pont métodos para produzir uma planta mutante e para gerar uma planta, planta, semente, rna, métodos para produzir uma célula, para duplicar um fragmento gênico, para substituir uma primeira sequência promotora, para inserir um elemento regulador em uma sequência de nucleotídeos e para inserir um íntron em uma sequência de nucleotídeos, planta de milho e célula vegetal
CN114009454A (zh) 2014-07-29 2022-02-08 孟山都技术公司 用于控制昆虫害虫的组合物和方法
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
CN108064288B (zh) 2015-01-22 2021-11-26 孟山都技术公司 用于控制叶甲属的组合物和方法
WO2016160389A1 (en) 2015-03-27 2016-10-06 E I Du Pont De Nemours And Company Soybean u6 small nuclear rna gene promoters and their use in constitutive expression of small rna genes in plants
AU2016270870A1 (en) 2015-06-02 2018-01-04 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
DK4144844T3 (da) 2015-10-12 2025-11-24 Dupont Us Holding Llc Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf
CN108138155A (zh) 2015-10-20 2018-06-08 先锋国际良种公司 经由指导cas系统恢复非功能性基因产物的功能及使用方法
GB201521101D0 (en) 2015-11-30 2016-01-13 Koninklijke Nederlandse Akademie Van Wetenschappen Transduction buffer
US20180362961A1 (en) 2015-12-11 2018-12-20 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
EP3708665A1 (en) 2015-12-18 2020-09-16 Danisco US Inc. Methods and compositions for t-rna based guide rna expression
KR20250117482A (ko) 2016-02-09 2025-08-04 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개된 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
US20190161742A1 (en) 2016-03-11 2019-05-30 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
BR112018076027A2 (pt) 2016-06-14 2019-03-26 Pioneer Hi-Bred International, Inc. método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
US20190100745A1 (en) 2016-06-20 2019-04-04 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
US11661624B2 (en) 2017-03-30 2023-05-30 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
CN110891965A (zh) 2017-04-24 2020-03-17 杜邦营养生物科学有限公司 植物中使用的抗crispr蛋白的方法和组合物
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
EP3630973A1 (en) 2017-05-31 2020-04-08 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
KR102510576B1 (ko) 2017-09-19 2023-03-14 트로픽 바이오사이언시즈 유케이 리미티드 유전자 발현을 침묵시키기 위한 식물의 비-암호화 rna 분자의 특이성 변형
EP3694561A4 (en) * 2017-10-12 2021-08-04 Mayo Foundation for Medical Education and Research METHODS AND MATERIALS FOR GENEDITATION
KR102705104B1 (ko) 2017-12-15 2024-09-09 다니스코 유에스 인크. Cas9 변이체 및 사용 방법
AU2019207703B2 (en) 2018-01-09 2024-11-14 Cibus Europe B.V. Shatterproof genes and mutations
AU2019210169B2 (en) * 2018-01-22 2024-10-31 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Biological nitrogen fixation in crops
CN112020554B (zh) 2018-02-23 2024-10-22 先锋国际良种公司 新颖cas9直系同源物
BR112020018644A2 (pt) 2018-03-12 2020-12-29 Pioneer Hi-Bred International, Inc. Uso de fatores morfogênicos para o aprimoramento de edição de genes
CA3095047A1 (en) 2018-04-04 2019-10-10 Cibus Us Llc Fad2 genes and mutations
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
CA3102978A1 (en) 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Nucleic acid constructs and methods of using same
IL279271B2 (en) 2018-06-07 2025-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Methods for Cannabis Regeneration and Transformation
BR112021011372A2 (pt) 2018-12-14 2021-08-31 Pioneer Hi-Bred International, Inc. Novos sistemas crispr-cas para edição de genoma
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
EP4110905A4 (en) 2020-02-24 2024-03-27 Pioneer Hi-Bred International, Inc. HOMOLOGOUS INTRAGENOMIC RECOMBINATION
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
GB202109586D0 (en) 2021-07-02 2021-08-18 Tropic Biosciences Uk Ltd Method for editing banana genes
US20240287535A1 (en) 2021-07-02 2024-08-29 Tropic Biosciences UK Limited Delay or prevention of browning in banana fruit
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
WO2023102393A1 (en) 2021-11-30 2023-06-08 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
EP4569122A2 (en) 2022-08-09 2025-06-18 Pioneer Hi-Bred International, Inc. Guide polynucleotide multiplexing
WO2024123786A1 (en) 2022-12-06 2024-06-13 Pioneer Hi-Bred International, Inc. Methods and compositions for co-delivery of t-dnas expressing multiple guide polynucleotides into plants
EP4684008A1 (en) 2023-03-20 2026-01-28 Pioneer Hi-Bred International, Inc. Cas polypeptides with altered pam recognition
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
WO2025259976A1 (en) 2024-06-14 2025-12-18 Pioneer Hi-Bred International, Inc. Deamination polyproteins and methods of use thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0001609B1 (de) 1977-10-20 1982-08-04 Skf Kugellagerfabriken Gmbh Verfahren zur Herstellung eines Wälzlagers mit einem in einer Aussenhülse angeordnetem Käfig und Käfig zur Durchführung des Verfahrens
DE19618797C2 (de) 1996-05-10 2000-03-23 Bertling Wolf Vehikel zum Transport molekularer Substanz
AT409381B (de) 2000-03-03 2002-07-25 Gloessl Josef Dr Xylosyltransferase-gen
KR20040053645A (ko) 2002-12-17 2004-06-24 학교법인 성균관대학 양이온성 올리고펩타이드를 이용한 식물 형질전환법
US20040147027A1 (en) * 2003-01-28 2004-07-29 Troy Carol M. Complex for facilitating delivery of dsRNA into a cell and uses thereof
KR100568457B1 (ko) 2003-07-22 2006-04-07 학교법인 성균관대학 양이온성 올리고펩타이드를 이용한 식물체로의 ｒｎａ전달 기법
US8507277B2 (en) * 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
WO2005117928A1 (en) 2004-05-30 2005-12-15 Cemines, Inc. Compositions and methods for the treatment of skin cancer

Also Published As

Publication number Publication date
CN101889091A (zh) 2010-11-17
WO2008148223A1 (en) 2008-12-11
AU2008258254A1 (en) 2008-12-11
EP2181193B1 (en) 2017-10-25
BRPI0814244B1 (pt) 2018-10-09
CN101889091B (zh) 2014-02-19
US8680366B2 (en) 2014-03-25
US9382548B2 (en) 2016-07-05
EP2181193A1 (en) 2010-05-05
US20140182012A1 (en) 2014-06-26
US20110035836A1 (en) 2011-02-10
AU2008258254B2 (en) 2014-07-03
MX2009013325A (es) 2010-05-20
CA2693525C (en) 2020-09-29
ZA200908635B (en) 2010-08-25
EP2181193A4 (en) 2010-08-04
CA2693525A1 (en) 2008-12-11
JP2010532159A (ja) 2010-10-07
RU2485180C2 (ru) 2013-06-20
RU2009148877A (ru) 2011-07-20

Similar Documents

Publication Publication Date Title
BRPI0814244A2 (pt) Método para obtenção de uma planta geneticamente engenheirada
BRPI0911232A2 (pt) Métodos e composições para melhorar a saúde da planta
BRPI0718094A2 (pt) Sistema de guinada para uma turbina eólica
BRPI0811747A2 (pt) Métodos e composições para a produção de solventes
DK3156488T3 (da) Brassica-plante, der omfatter en mutant-ind-allel (indehiscent - ikke-opspringende)
BRPI0814359A2 (pt) Método
BRPI0817226A2 (pt) Método
BRPI0817726A2 (pt) Método
PL2109704T3 (pl) Siłownia wiatrowa
FI20075721L (fi) Menetelmä pyrolysoinnin toteuttamiseksi
BRPI0821003A2 (pt) Método para aumentar a imunorreatividade
EP2148561A4 (en) PLANTER
BRPI0806960A2 (pt) Molécula de dsrna, coleção de moléculas de dsrna, planta transgência, e, método para preparar uma planta transgênica.
BRPI0807020A2 (pt) Método para melhorar a saúde da planta de pelo menos uma variedade de planta
BRPI0814775A2 (pt) Vigor de planta crescente
BRPI0718379A2 (pt) Método para intensificar a produtividade intrínseca de uma planta
BRPI0815810A2 (pt) Métodos para incorporação de genes múltiplos em uma planta de cultura
BRPI0913898A2 (pt) dispositivo de trituração para uma planta de trituração
BRPI0910471A2 (pt) planta e aparelhos para a criação de tampas do tipo coroa
BRPI0914419A2 (pt) Planta geneticamente modificada, e, métodos para produzir a mesma, e para produzir capsinóide
BR112012003690A2 (pt) estrutura para cobertura de solo e uma planta e método para produzir a dita estrutura
BRPI0916223A2 (pt) planta de cimento e método para operar uma planta de cimento
BRPI0815130A2 (pt) composição, e, método para fabricar uma composição
BR112012003139A2 (pt) Método para produzir uma planta híbrida, planta, material de planta ou semente
BRPI1008808A2 (pt) método para preparar uma planta tolerante ao estresse abiótico, planta transgênica, e, semente transgênica

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 09/10/2018, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 14A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2674 DE 05-04-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.