BRPI0721238A2 - Método de purificação da apolipoproteina a-1 - Google Patents
Método de purificação da apolipoproteina a-1Info
- Publication number
- BRPI0721238A2 BRPI0721238A2 BRPI0721238-0A BRPI0721238A BRPI0721238A2 BR PI0721238 A2 BRPI0721238 A2 BR PI0721238A2 BR PI0721238 A BRPI0721238 A BR PI0721238A BR PI0721238 A2 BRPI0721238 A2 BR PI0721238A2
- Authority
- BR
- Brazil
- Prior art keywords
- solution
- apoa
- apolipoprotein
- protein
- purification method
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 102000005666 Apolipoprotein A-I Human genes 0.000 title abstract 2
- 108010059886 Apolipoprotein A-I Proteins 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 239000000243 solution Substances 0.000 abstract 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 3
- 239000004202 carbamide Substances 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- 125000000129 anionic group Chemical group 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 2
- 238000010828 elution Methods 0.000 abstract 2
- 239000012460 protein solution Substances 0.000 abstract 2
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/775—Apolipopeptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
Método de purificação da apolipoproteína a-1. Um método de purificação da apoliproteína a-1 incluindo a mistura da fração de plasma iv adquirida pelo método de fracionamento por etanol de cohn com uma solução de uréia de 1-8m para formar uma solução de pré-tratamento de fração iv, carga da solução de pré-tratamento a uma primeira coluna de cromatografia aniônica e posterior eluição com uma solução de uréia de 1-8m para obter uma solução de proteína apoa-1, e carga da solução de proteína apoa-1 em uma segunda coluna de cromatografia aniônica e eluição com uma solução de uréia de 0-1m para obter proteína apoa-1 pura.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610147503A CN100586958C (zh) | 2006-12-20 | 2006-12-20 | 高纯度载脂蛋白a-i的制备方法 |
| CN200610147503.7 | 2006-12-20 | ||
| PCT/US2007/020258 WO2008088403A2 (en) | 2006-12-20 | 2007-09-19 | Method of purifying apolipoprotein a-1 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI0721238A2 true BRPI0721238A2 (pt) | 2015-06-23 |
| BRPI0721238B1 BRPI0721238B1 (pt) | 2018-05-29 |
| BRPI0721238B8 BRPI0721238B8 (pt) | 2021-05-25 |
Family
ID=39565747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0721238A BRPI0721238B8 (pt) | 2006-12-20 | 2007-09-19 | método de purificação da apolipoproteina a-1 |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP2125861B1 (pt) |
| JP (1) | JP2010513480A (pt) |
| KR (1) | KR101363106B1 (pt) |
| CN (1) | CN100586958C (pt) |
| AU (1) | AU2009202827B8 (pt) |
| BR (1) | BRPI0721238B8 (pt) |
| CA (1) | CA2673516C (pt) |
| EG (1) | EG26516A (pt) |
| MX (1) | MX2009006766A (pt) |
| MY (1) | MY154897A (pt) |
| RU (1) | RU2453555C2 (pt) |
| TW (1) | TWI357347B (pt) |
| WO (1) | WO2008088403A2 (pt) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120177610A1 (en) * | 2007-09-19 | 2012-07-12 | Kieu Hoang | Manufacturing and Purification Processes of Complex Protein found in Fraction IV to make a separated Apo, Transferrin , and Alpha 1 Anti strepsin (A1AT) or A combined Transferrin / Apo/Human Albumin/A1AT and all new found proteins |
| CN102127165B (zh) * | 2010-01-15 | 2013-09-04 | 上海莱士血液制品股份有限公司 | 一种从血浆组分四沉淀中制备高纯ApoA-I的生产工艺 |
| HUE032560T2 (en) * | 2010-06-30 | 2017-09-28 | Csl Ltd | Dissolved high density lipoprotein formulation and preparation process |
| CN102731642B (zh) * | 2011-04-14 | 2014-01-29 | 上海莱士血液制品股份有限公司 | 从人血浆组分四沉淀制备高纯Apoa-I的生产工艺 |
| JP5924624B2 (ja) * | 2011-04-28 | 2016-05-25 | 国立研究開発法人理化学研究所 | 生物材料を透明化する方法、及びその利用 |
| JP6044900B2 (ja) | 2011-05-20 | 2016-12-14 | 国立研究開発法人理化学研究所 | 生物材料用透明化試薬、及びその利用 |
| US20130053551A1 (en) | 2011-08-25 | 2013-02-28 | Hoffman-La Roche. Inc. | Cation and anion exchange chromatography method |
| CN103505720A (zh) * | 2012-06-30 | 2014-01-15 | 复旦大学 | 载脂蛋白a-i在制备预防和治疗肝病代谢综合征药物中的用途 |
| US9534029B2 (en) | 2012-10-03 | 2017-01-03 | Csl Behring Ag | Method of purifying proteins |
| WO2015022883A1 (ja) | 2013-08-14 | 2015-02-19 | 独立行政法人理化学研究所 | 光透過性に優れた生物材料を調製するための組成物およびその利用 |
| CN107033237B (zh) * | 2017-05-11 | 2021-07-20 | 深圳市卫光生物制品股份有限公司 | 一种人血浆载脂蛋白a-i的分离纯化方法 |
| CN110590935B (zh) * | 2019-09-23 | 2024-01-30 | 华兰生物工程重庆有限公司 | 人血白蛋白去除多聚体工艺 |
| CN111153985B (zh) * | 2020-01-20 | 2023-06-13 | 宁波赛珀生物技术有限公司 | 血清载脂蛋白a-ii的分离纯化方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1752187A3 (ru) * | 1990-12-11 | 1992-07-30 | А.Б.Сигалов | Способ делипидировани лиофилизированных липопротеинов высокой плотности из сыворотки крови человека |
| WO1993000443A1 (en) * | 1991-06-26 | 1993-01-07 | Bio-Technology General Corp. | Purification of recombinant apolipoprotein e from bacteria |
| SE9500778D0 (sv) * | 1995-03-03 | 1995-03-03 | Pharmacia Ab | Process for producing a protein |
| SE9603068D0 (sv) * | 1996-08-23 | 1996-08-23 | Pharmacia & Upjohn Ab | Process for purifying a protein |
| HK1082661A1 (zh) * | 2002-07-30 | 2006-06-16 | Esperion Therapeutics Inc. | 使用非人类动物载脂蛋白a-i蛋白的方法 |
-
2006
- 2006-12-20 CN CN200610147503A patent/CN100586958C/zh active Active
-
2007
- 2007-09-19 MX MX2009006766A patent/MX2009006766A/es active IP Right Grant
- 2007-09-19 BR BRPI0721238A patent/BRPI0721238B8/pt not_active IP Right Cessation
- 2007-09-19 MY MYPI20092514A patent/MY154897A/en unknown
- 2007-09-19 KR KR1020097014720A patent/KR101363106B1/ko not_active Expired - Fee Related
- 2007-09-19 JP JP2009542756A patent/JP2010513480A/ja active Pending
- 2007-09-19 CA CA2673516A patent/CA2673516C/en active Active
- 2007-09-19 RU RU2009127816/04A patent/RU2453555C2/ru active
- 2007-09-19 WO PCT/US2007/020258 patent/WO2008088403A2/en not_active Ceased
- 2007-09-19 EP EP07861342.9A patent/EP2125861B1/en not_active Not-in-force
- 2007-11-30 TW TW096145616A patent/TWI357347B/zh not_active IP Right Cessation
-
2009
- 2009-06-18 EG EG2009060940A patent/EG26516A/en active
- 2009-07-14 AU AU2009202827A patent/AU2009202827B8/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008088403A2 (en) | 2008-07-24 |
| JP2010513480A (ja) | 2010-04-30 |
| CN100586958C (zh) | 2010-02-03 |
| AU2009202827A8 (en) | 2012-03-08 |
| CA2673516C (en) | 2013-10-08 |
| BRPI0721238B1 (pt) | 2018-05-29 |
| TW200922675A (en) | 2009-06-01 |
| EP2125861A4 (en) | 2011-02-02 |
| AU2009202827B8 (en) | 2012-03-08 |
| AU2009202827B2 (en) | 2011-12-15 |
| RU2453555C2 (ru) | 2012-06-20 |
| AU2009202827A1 (en) | 2009-08-06 |
| EP2125861A2 (en) | 2009-12-02 |
| KR20090116706A (ko) | 2009-11-11 |
| MY154897A (en) | 2015-08-28 |
| KR101363106B1 (ko) | 2014-02-13 |
| CN101205250A (zh) | 2008-06-25 |
| WO2008088403A3 (en) | 2008-11-06 |
| MX2009006766A (es) | 2009-08-12 |
| CA2673516A1 (en) | 2008-07-24 |
| EP2125861B1 (en) | 2014-07-16 |
| TWI357347B (en) | 2012-02-01 |
| BRPI0721238B8 (pt) | 2021-05-25 |
| RU2009127816A (ru) | 2011-01-27 |
| EG26516A (en) | 2014-01-08 |
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