BRPI0612423A2 - estabelecimento de linhagem de cÉlulas-tronco embrionÁrias humanas usando cÉlulas de mamÍferos - Google Patents
estabelecimento de linhagem de cÉlulas-tronco embrionÁrias humanas usando cÉlulas de mamÍferosInfo
- Publication number
- BRPI0612423A2 BRPI0612423A2 BRPI0612423-2A BRPI0612423A BRPI0612423A2 BR PI0612423 A2 BRPI0612423 A2 BR PI0612423A2 BR PI0612423 A BRPI0612423 A BR PI0612423A BR PI0612423 A2 BRPI0612423 A2 BR PI0612423A2
- Authority
- BR
- Brazil
- Prior art keywords
- embryonic stem
- cells
- human embryonic
- preparation
- establishment
- Prior art date
Links
- 210000001671 embryonic stem cell Anatomy 0.000 title abstract 6
- 210000004962 mammalian cell Anatomy 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 5
- 238000002360 preparation method Methods 0.000 abstract 4
- 210000003981 ectoderm Anatomy 0.000 abstract 2
- 210000001900 endoderm Anatomy 0.000 abstract 2
- 210000003716 mesoderm Anatomy 0.000 abstract 2
- LAQPKDLYOBZWBT-NYLDSJSYSA-N (2s,4s,5r,6r)-5-acetamido-2-{[(2s,3r,4s,5s,6r)-2-{[(2r,3r,4r,5r)-5-acetamido-1,2-dihydroxy-6-oxo-4-{[(2s,3s,4r,5s,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}hexan-3-yl]oxy}-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy}-4-hydroxy-6-[(1r,2r)-1,2,3-trihydrox Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]([C@@H](NC(C)=O)C=O)[C@@H]([C@H](O)CO)O[C@H]1[C@H](O)[C@@H](O[C@]2(O[C@H]([C@H](NC(C)=O)[C@@H](O)C2)[C@H](O)[C@H](O)CO)C(O)=O)[C@@H](O)[C@@H](CO)O1 LAQPKDLYOBZWBT-NYLDSJSYSA-N 0.000 abstract 1
- 102000007469 Actins Human genes 0.000 abstract 1
- 108010085238 Actins Proteins 0.000 abstract 1
- 102100027211 Albumin Human genes 0.000 abstract 1
- 108010088751 Albumins Proteins 0.000 abstract 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 abstract 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 abstract 1
- 102100028072 Fibroblast growth factor 4 Human genes 0.000 abstract 1
- 102100023374 Forkhead box protein M1 Human genes 0.000 abstract 1
- 101150092640 HES1 gene Proteins 0.000 abstract 1
- 101001060274 Homo sapiens Fibroblast growth factor 4 Proteins 0.000 abstract 1
- 101000907578 Homo sapiens Forkhead box protein M1 Proteins 0.000 abstract 1
- 101001111338 Homo sapiens Neurofilament heavy polypeptide Proteins 0.000 abstract 1
- 101000777245 Homo sapiens Undifferentiated embryonic cell transcription factor 1 Proteins 0.000 abstract 1
- 102000005712 Keratin-8 Human genes 0.000 abstract 1
- 108010070511 Keratin-8 Proteins 0.000 abstract 1
- 102000013013 Member 2 Subfamily G ATP Binding Cassette Transporter Human genes 0.000 abstract 1
- 108010090306 Member 2 Subfamily G ATP Binding Cassette Transporter Proteins 0.000 abstract 1
- 101100224389 Mus musculus Dppa5a gene Proteins 0.000 abstract 1
- 101100284799 Mus musculus Hesx1 gene Proteins 0.000 abstract 1
- 101100310657 Mus musculus Sox1 gene Proteins 0.000 abstract 1
- 101710150336 Protein Rex Proteins 0.000 abstract 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 abstract 1
- 102100035459 Pyruvate dehydrogenase protein X component, mitochondrial Human genes 0.000 abstract 1
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 abstract 1
- 101150052863 THY1 gene Proteins 0.000 abstract 1
- 108010017842 Telomerase Proteins 0.000 abstract 1
- 101001023030 Toxoplasma gondii Myosin-D Proteins 0.000 abstract 1
- 102100031278 Undifferentiated embryonic cell transcription factor 1 Human genes 0.000 abstract 1
- 230000000747 cardiac effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
ESTABELECIMENTO DE LINHAGEM DE CÉLULAS-TRONCO EMBRIONÁRIAS HUMANAS USANDO CÉLULAS DE MAMÍFEROS, onde são reveladas preparações purificadas de células-tronco embrionárias humanas com determinadas características específicas da população. Esta preparação é caracterizada por uma expressão positiva dos seguintes marcadores de superficie celular pluripotentes: SSEA-1 (-); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); fosfatase alcalina (+), bem como um conjunto de marcadores celulares ES incluindo Oct-4, Nanog, Rex1, Sox2, Thy1, FGF4, ABCG2, Dppa5, UTF1, Cripto1, hTERT, Conexina-43 e Conexina-45. As células da preparação são negativas para marcadores específicos da linhagem como Queratina 8, Sox- 1, NFH (ectoderme), MyoD, brachyury, actina cardíaca (mesoderme), HNF-3 beta, albumina e PDX1 (endoderme). As células da preparação são células-tronco embrionárias humanas, possuem cariótipos normais, exibem alta atividade da telomerase e continuam a proliferar em um estado indiferenciado após cultura contínua de mais de 40 passagens. A linhagem de células-tronco embrionárias Relicell® Hes 1 também possui a habilidade, ao longo da cultura, de diferenciar os tipos de tecidos e células derivados de todas as três camadas de origem embrionárias (endoderme, mesoderme e ectoderme). Também são revelados métodos para isolar a linhagem de células-tronco embrionárias humanas.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN595MU2005 | 2005-05-17 | ||
| US11/436,193 US7811817B2 (en) | 2005-05-17 | 2006-05-17 | Establishment of a human embryonic stem cell line using mammalian cells |
| PCT/IN2006/000169 WO2007020655A1 (en) | 2005-05-17 | 2006-05-17 | Establishment of a human embryonic stem cell line using mammalian cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0612423A2 true BRPI0612423A2 (pt) | 2009-01-27 |
Family
ID=37573810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0612423-2A BRPI0612423A2 (pt) | 2005-05-17 | 2006-05-17 | estabelecimento de linhagem de cÉlulas-tronco embrionÁrias humanas usando cÉlulas de mamÍferos |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7811817B2 (pt) |
| EP (1) | EP1893748A1 (pt) |
| JP (1) | JP2009517001A (pt) |
| CN (1) | CN101180389A (pt) |
| AU (1) | AU2006281032B2 (pt) |
| BR (1) | BRPI0612423A2 (pt) |
| IL (1) | IL187352A (pt) |
| RU (1) | RU2007146777A (pt) |
| WO (1) | WO2007020655A1 (pt) |
| ZA (1) | ZA200709583B (pt) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060183221A1 (en) * | 2003-03-31 | 2006-08-17 | Schulz Thomas C | Method for neural differentiation of embryonic stem cells using protease passaging techniques |
| SG177946A1 (en) | 2005-08-29 | 2012-02-28 | Technion Res & Dev Foundation | Media for culturing stem cells |
| EP1991664B1 (en) * | 2006-02-16 | 2018-03-28 | Ospedale San Raffaele S.r.l. | Skeletal muscle periangioblasts and cardiac mesangioblasts, method for isolation and uses thereof |
| DK3441459T3 (da) | 2006-08-02 | 2021-06-07 | Technion Res & Dev Foundation | Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur |
| US20090220996A1 (en) * | 2007-03-06 | 2009-09-03 | Reliance Life Sciences Pvt Ltd. | In vitro Assay Methods for Classifying Embryotoxicity of Compounds |
| CN101878295A (zh) | 2007-10-12 | 2010-11-03 | 先进细胞技术公司 | 制备rpe细胞和rpe细胞的组合物的改良方法 |
| WO2009087681A2 (en) * | 2007-12-28 | 2009-07-16 | Reliance Life Sciences Pvt. Ltd. | Methods for characterisation of mammalian embryonic stem cells by multiplex pcr |
| WO2009089067A2 (en) * | 2008-01-10 | 2009-07-16 | The General Hospital Corporation | Methods and compositions for identifying undifferentiated stem cells and assessing cell health |
| EP2362899A1 (en) | 2008-10-31 | 2011-09-07 | Katholieke Universiteit Leuven | Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells |
| CN101760450B (zh) * | 2008-11-18 | 2013-04-10 | 中国科学院上海生命科学研究院 | 一种白蛋白/角蛋白双表达小鼠胚胎干细胞系及其建立方法 |
| US9550975B2 (en) * | 2009-07-15 | 2017-01-24 | Mari Dezawa | SSEA-3 pluripotent stem cell isolated from body tissue |
| ES2932664T3 (es) | 2009-11-12 | 2023-01-23 | Technion Res & Dev Foundation | Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en estado no diferenciado |
| CA2781149A1 (en) | 2009-11-17 | 2011-05-26 | Advanced Cell Technology, Inc. | Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells |
| US9090878B2 (en) | 2010-06-17 | 2015-07-28 | Katholieke Universiteit Leuven | Methods for differentiating cells into hepatic stellate cells and hepatic sinusoidal endothelial cells, cells produced by the methods, and methods for using the cells |
| CN102793911B (zh) | 2011-05-24 | 2015-12-16 | 李建远 | 重组人Prx-6蛋白在治疗角膜损伤方面的用途 |
| WO2012135253A1 (en) * | 2011-03-29 | 2012-10-04 | Geron Corporation | Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells |
| US10744183B2 (en) | 2013-03-15 | 2020-08-18 | Elixirgen Therapeutics, Inc. | Methods of using ZSCAN4 for rejuvenating human cells |
| EP3423158B1 (en) | 2016-02-24 | 2023-11-15 | The Rockefeller University | Embryonic cell-based therapeutic candidate screening systems, models for huntington's disease and uses thereof |
| CN111044722A (zh) * | 2020-01-01 | 2020-04-21 | 南京优而生物科技发展有限公司 | 一种辅助生殖技术用鼠胚试验囊胚细胞染色和计数试剂盒 |
| CN111197028A (zh) * | 2020-03-09 | 2020-05-26 | 崔立峰 | 一种人体脂肪干细胞的培养方法 |
| CN112813020B (zh) * | 2020-09-10 | 2022-08-30 | 中国科学院动物研究所 | 具有特定hla配型的人胚干细胞 |
| CN113913294A (zh) * | 2021-10-08 | 2022-01-11 | 南京优而生物科技发展有限公司 | 一种能诱导干细胞分泌细胞因子的方法及其培养装置 |
| CN117912551B (zh) * | 2023-01-07 | 2025-11-07 | 杭州链康医学检验实验室有限公司 | 一种构建多物种细胞-整合基因表达谱的方法、系统、设备和介质 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| CA2458362A1 (en) | 2001-08-23 | 2003-03-06 | Reliance Life Sciences Pvt., Ltd. | Isolation of inner cell mass for the establishment of human embryonic stem cell (hesc) lines |
| US20030073234A1 (en) * | 2001-10-12 | 2003-04-17 | Michal Amit | Clonal human embryonic stem cell lines and methods of generating same |
| KR100763477B1 (ko) | 2003-03-12 | 2007-10-04 | 리라이언스 라이프 사이언시스 프라이빗. 리미티드 | 사람 배아 줄기세포로부터 최종적으로 분화된 도파민작용성 뉴런의 유도 |
| US7820439B2 (en) | 2003-09-03 | 2010-10-26 | Reliance Life Sciences Pvt Ltd. | In vitro generation of GABAergic neurons from pluripotent stem cells |
-
2006
- 2006-05-17 WO PCT/IN2006/000169 patent/WO2007020655A1/en not_active Ceased
- 2006-05-17 JP JP2008511867A patent/JP2009517001A/ja active Pending
- 2006-05-17 US US11/436,193 patent/US7811817B2/en not_active Expired - Fee Related
- 2006-05-17 RU RU2007146777/13A patent/RU2007146777A/ru unknown
- 2006-05-17 BR BRPI0612423-2A patent/BRPI0612423A2/pt not_active IP Right Cessation
- 2006-05-17 EP EP06809923A patent/EP1893748A1/en not_active Withdrawn
- 2006-05-17 AU AU2006281032A patent/AU2006281032B2/en not_active Ceased
- 2006-05-17 CN CNA2006800173875A patent/CN101180389A/zh active Pending
-
2007
- 2007-11-07 ZA ZA200709583A patent/ZA200709583B/xx unknown
- 2007-11-13 IL IL187352A patent/IL187352A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2006281032B2 (en) | 2010-09-02 |
| US7811817B2 (en) | 2010-10-12 |
| ZA200709583B (en) | 2009-03-25 |
| WO2007020655A1 (en) | 2007-02-22 |
| AU2006281032A1 (en) | 2007-02-22 |
| EP1893748A1 (en) | 2008-03-05 |
| RU2007146777A (ru) | 2009-06-27 |
| WO2007020655A8 (en) | 2008-01-17 |
| IL187352A (en) | 2011-08-31 |
| CN101180389A (zh) | 2008-05-14 |
| JP2009517001A (ja) | 2009-04-30 |
| IL187352A0 (en) | 2008-04-13 |
| US20060286544A1 (en) | 2006-12-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 5A E 6A ANUIDADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2161 DE 05/06/2012. |
|
| B15K | Others concerning applications: alteration of classification |
Ipc: C12N 5/0735 (2010.01) |