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BRPI0406265B8 - método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais - Google Patents

método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais

Info

Publication number
BRPI0406265B8
BRPI0406265B8 BRPI0406265A BRPI0406265A BRPI0406265B8 BR PI0406265 B8 BRPI0406265 B8 BR PI0406265B8 BR PI0406265 A BRPI0406265 A BR PI0406265A BR PI0406265 A BRPI0406265 A BR PI0406265A BR PI0406265 B8 BRPI0406265 B8 BR PI0406265B8
Authority
BR
Brazil
Prior art keywords
typing
monoclonal proteins
antibodies
proteins
kit
Prior art date
Application number
BRPI0406265A
Other languages
English (en)
Inventor
Robert Frédéric
Andre Régis
Original Assignee
Sebia Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sebia Sa filed Critical Sebia Sa
Publication of BRPI0406265A publication Critical patent/BRPI0406265A/pt
Publication of BRPI0406265B1 publication Critical patent/BRPI0406265B1/pt
Publication of BRPI0406265B8 publication Critical patent/BRPI0406265B8/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"método para análise eletroforética capilar, métodos para pesquisa e tipagem de proteínas monoclonais, processo para preparação de anticorpos modificados, anticorpos modificados, kit para detecção e tipagem de proteínas monoclonais e uso de anticorpos". a presente invenção trata de um método para a análise eletroforética capilar de uma amostra biológica que compreende a utilização de anticorpos modificados carregados negativamente de modo tal que eles migrem para uma zona situada fora da zona de migração das proteínas da amostra biológica quando são separadas durante a eletroforese, sendo que os referidos anticorpos possuem uma especificidade antigênica para uma proteína alvo determinada.
BRPI0406265A 2003-11-12 2004-11-10 método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais BRPI0406265B8 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0313246A FR2862134B1 (fr) 2003-11-12 2003-11-12 Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement

Publications (3)

Publication Number Publication Date
BRPI0406265A BRPI0406265A (pt) 2005-08-23
BRPI0406265B1 BRPI0406265B1 (pt) 2016-11-01
BRPI0406265B8 true BRPI0406265B8 (pt) 2021-07-27

Family

ID=34429975

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0406265A BRPI0406265B8 (pt) 2003-11-12 2004-11-10 método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais

Country Status (15)

Country Link
US (1) US8609435B2 (pt)
EP (1) EP1531332B1 (pt)
JP (1) JP4693392B2 (pt)
CN (1) CN1641353B (pt)
AR (1) AR046834A1 (pt)
AT (1) ATE418735T1 (pt)
BR (1) BRPI0406265B8 (pt)
CY (1) CY1110461T1 (pt)
DE (1) DE602004018598D1 (pt)
DK (1) DK1531332T3 (pt)
ES (1) ES2318251T3 (pt)
FR (1) FR2862134B1 (pt)
PL (1) PL1531332T3 (pt)
PT (1) PT1531332E (pt)
SI (1) SI1531332T1 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2878033B1 (fr) * 2004-11-18 2007-05-04 Sebia Sa Procede d'analyse de l'hemoglobine par electrophorese capillaire, trousse pour electrophorese capillaire et utilisation de ralentisseur de flux dans un tel procede
CN101300488B (zh) * 2005-09-02 2012-09-05 和光纯药工业株式会社 复合物的形成方法和分离方法
US8337685B2 (en) * 2007-11-30 2012-12-25 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Serum components that bind to threat agents
EP2184606B1 (en) 2008-11-03 2015-06-24 Helena Laboratories (UK) Ltd. Immunodisplacement Electrophoresis
GB201010405D0 (en) * 2010-06-21 2010-08-04 Binding Site Group The Ltd Assay
US20120077184A1 (en) * 2010-09-28 2012-03-29 Starkdx Incorporated Electromagnetic multiplex assay biosensor
EP2877846A1 (en) * 2012-05-29 2015-06-03 Health Diagnostic Laboratory, Inc. Compsition and method for gel electrophoresis with in-situ clalibration
DK3276351T3 (da) 2016-07-27 2020-07-20 Sebia Sa Immunofixationselektroforese-fremgangsmåde med target-komponenter on-gel immunoforskydning
GB201709387D0 (en) * 2017-06-13 2017-07-26 Genetic Microdevices Ltd Method
BR112021013716A2 (pt) * 2019-01-31 2021-09-21 Regeneron Pharmaceuticals, Inc. Análise microfluídica nativa ce-ms de heterogeneidade de carga de anticorpo
MX2021014171A (es) * 2019-05-21 2022-01-04 Regeneron Pharma Metodos para identificar y cuantificar proteinas de la celula huesped.
IT202000006694A1 (it) * 2020-03-31 2021-10-01 Bioci Di Ciaiolo Carlo Procedimento per la rivelazione di componenti immunoglobuliniche monoclonali in un fluido biologico e relativo kit
CN113552370B (zh) * 2021-09-23 2021-12-28 北京小蝇科技有限责任公司 一种毛细管免疫分型单克隆免疫球蛋白定量分析方法

Family Cites Families (20)

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JP2564540B2 (ja) * 1987-04-01 1996-12-18 株式会社日立製作所 免疫分析方法
US5010176A (en) * 1988-11-10 1991-04-23 Eli Lilly And Company Antibody-drug conjugates
US5169764A (en) * 1990-08-08 1992-12-08 Regeneron Pharmaceuticals, Inc. Multitrophic and multifunctional chimeric neurotrophic factors, and nucleic acids and plasmids encoding the chimeras
US5459272A (en) * 1990-10-16 1995-10-17 Research Corporation Technologies Quinoline-2-(carboxaldehyde) reagents for detection of primary amines
NL9100434A (nl) 1991-03-11 1992-10-01 Rijksuniversiteit Farmaceutische preparaten voor de bestrijding van virale infecties en immuundeficientieziekten, de bereiding en toepassing daarvan.
US5228960A (en) * 1992-07-17 1993-07-20 Beckman Instruments, Inc. Analysis of samples by capillary electrophoretic immunosubtraction
US5958202A (en) * 1992-09-14 1999-09-28 Perseptive Biosystems, Inc. Capillary electrophoresis enzyme immunoassay
US5567282A (en) * 1994-01-25 1996-10-22 Wang; Hann-Ping On-capillary electrophoretic immunosubtraction for classification and typing of M-proteins
DE69422604T2 (de) * 1994-03-08 2000-06-08 Zeptosens Ag, Witterwil Vorrichtung und Verfahren, die Bioaffinitätsassay und elektrophoretische Auftrennung kombinieren
US5431793A (en) * 1994-07-29 1995-07-11 Beckman Instruments, Inc. Quantitative analysis of glycosylated hemoglobin by immunocappillary electrophoresis
JP3596821B2 (ja) * 1994-09-09 2004-12-02 キヤノン株式会社 画像形成装置
US5985275A (en) * 1995-04-12 1999-11-16 New York Blood Center β-Lactoglobulin modified with aromatic anhydride compound for preventing HIV infection
US5630924A (en) * 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
US5753094A (en) * 1995-09-20 1998-05-19 Beckman Instruments, Inc. Borate storage buffer and sample diluent
JPH10307376A (ja) * 1997-03-05 1998-11-17 Fuji Photo Film Co Ltd ハロゲン化銀感光材料および画像形成方法
US6156179A (en) * 1998-07-09 2000-12-05 Bio-Rad Laboratories Computer directed identification of paraproteins
CA2380329A1 (en) 1999-08-10 2001-02-15 Christopher John Lienhart Non-aqueous liquid detergents with water-soluble low-density particles
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FR2819889B1 (fr) 2001-01-19 2003-08-01 Sebia Sa Procede d'electrophorese capillaire en solution libre a ph alcalin
FR2819888B1 (fr) 2001-01-19 2003-09-12 Sebia Sa Procede de separation de proteines par electrophorese capillaire et compositions de tampon pour electrophorese capillaire

Also Published As

Publication number Publication date
CN1641353A (zh) 2005-07-20
CN1641353B (zh) 2012-06-06
BRPI0406265A (pt) 2005-08-23
ATE418735T1 (de) 2009-01-15
US20050164302A1 (en) 2005-07-28
US8609435B2 (en) 2013-12-17
DK1531332T3 (da) 2009-04-14
CY1110461T1 (el) 2015-04-29
FR2862134B1 (fr) 2007-07-27
FR2862134A1 (fr) 2005-05-13
JP2005181299A (ja) 2005-07-07
ES2318251T3 (es) 2009-05-01
BRPI0406265B1 (pt) 2016-11-01
EP1531332A1 (fr) 2005-05-18
SI1531332T1 (sl) 2009-04-30
PT1531332E (pt) 2009-02-03
JP4693392B2 (ja) 2011-06-01
PL1531332T3 (pl) 2009-06-30
EP1531332B1 (fr) 2008-12-24
DE602004018598D1 (de) 2009-02-05
AR046834A1 (es) 2005-12-28

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