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BR9916840A - Integrated portable biological detection system - Google Patents

Integrated portable biological detection system

Info

Publication number
BR9916840A
BR9916840A BR9916840-5A BR9916840A BR9916840A BR 9916840 A BR9916840 A BR 9916840A BR 9916840 A BR9916840 A BR 9916840A BR 9916840 A BR9916840 A BR 9916840A
Authority
BR
Brazil
Prior art keywords
integrated portable
detection system
dna
biological detection
followed
Prior art date
Application number
BR9916840-5A
Other languages
Portuguese (pt)
Inventor
Jing Cheng
Lei Wu
Michael Heller
Ed Sheldon
Jonathan Diver
James P O'connell
Dan Smolko
Shila Jalali
David Willoughby
Original Assignee
Nanogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanogen Inc filed Critical Nanogen Inc
Publication of BR9916840A publication Critical patent/BR9916840A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Signal Processing (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Electrostatic Separation (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Patente de Invenção: <B>"SISTEMA DE DETECçãO BIOLóGICA PORTáTIL INTEGRADO"<D>. A presente invenção refere-se a separação de células bacterianas e cancerígenas de sangue humano periférico em chips eletrónicos microfabricados por dieletroforese. As células isoladas foram examinadas por coloração dos núcleos com corante fluorescente, seguida por formação de imagem fluorescente induzida por laser. DNA e RNA também foram liberados eletronicamente das células liberadas, e as sequências de marcadores específicos detectadas por amplificação de DNA, seguida por hibridização eletrónica para as sondas de captura imobilizadas. As tentativas no sentido da construção de um sistema "lab-on-a chip" são apresentadas, o que envolve a seleção de sondas de DNA, corantes, reagentes e estabelecimento de um protótipo do instrumento portátil completamente integrado.Invention Patent: <B> "INTEGRATED PORTABLE BIOLOGICAL DETECTION SYSTEM" <D>. The present invention relates to the separation of bacterial and cancer cells from peripheral human blood into electronic chips manufactured by dielectrophoresis. Isolated cells were examined by staining the nuclei with fluorescent dye, followed by laser-induced fluorescent imaging. DNA and RNA were also released electronically from the released cells, and the sequences of specific markers detected by DNA amplification, followed by electronic hybridization for the immobilized capture probes. Attempts to build a "lab-on-a chip" system are presented, which involves selecting DNA probes, dyes, reagents and establishing a fully integrated portable instrument prototype.

BR9916840-5A 1998-12-23 1999-12-22 Integrated portable biological detection system BR9916840A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11373098P 1998-12-23 1998-12-23
PCT/US1999/031098 WO2000037163A1 (en) 1998-12-23 1999-12-22 Integrated portable biological detection system

Publications (1)

Publication Number Publication Date
BR9916840A true BR9916840A (en) 2001-10-09

Family

ID=22351155

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9916840-5A BR9916840A (en) 1998-12-23 1999-12-22 Integrated portable biological detection system

Country Status (8)

Country Link
EP (1) EP1144092A4 (en)
JP (1) JP2002536962A (en)
KR (1) KR20010102961A (en)
AU (1) AU763514B2 (en)
BR (1) BR9916840A (en)
CA (1) CA2353461A1 (en)
NZ (1) NZ512087A (en)
WO (1) WO2000037163A1 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582421B2 (en) 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6379897B1 (en) 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
CN1181337C (en) 2000-08-08 2004-12-22 清华大学 Method for manipulating solid molecules in microfluidic system and related kits
US6884395B2 (en) 2000-05-12 2005-04-26 Gyros Ab Integrated microfluidic disc
US6251609B1 (en) * 2000-07-27 2001-06-26 Becton, Dickinson And Company Amplification and detection of Legionella pneumophila targeting the mip gene
US6277582B1 (en) * 2000-07-27 2001-08-21 Becton, Dickinson And Company Amplification and detection of mycoplasma pneumoniae targeting the P1 gene
US6261785B1 (en) * 2000-07-27 2001-07-17 Becton, Dickinson And Company Amplification and detection of Bordetella pertussis
CN100495030C (en) 2000-09-30 2009-06-03 清华大学 Multi-force operator and use thereof
JP4505776B2 (en) * 2001-01-19 2010-07-21 凸版印刷株式会社 Gene detection system, gene detection apparatus equipped with the same, detection method, and gene detection chip
KR100393131B1 (en) * 2001-03-15 2003-07-31 케이맥(주) Method for crystalizing proteins in nano scale, and chamber for crystalizing proteins and chip comprising the same
US6890409B2 (en) 2001-08-24 2005-05-10 Applera Corporation Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
WO2003080868A1 (en) * 2002-03-27 2003-10-02 Jae-Chern Yoo Bio-disc, bio-driver apparatus, and assay method using the same
US20040058423A1 (en) * 2002-05-03 2004-03-25 Nancy Albritton Fast electrical lysis of cells and rapid collection of the contents thereof using capillary electrophoresis
KR100441893B1 (en) * 2002-08-03 2004-07-27 학교법인 포항공과대학교 Nucleic acid separation device using MEMS component
JP4216018B2 (en) * 2002-08-26 2009-01-28 賢二 安田 Nucleic acid recovery chip and nucleic acid recovery device
WO2004029221A2 (en) 2002-09-27 2004-04-08 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
BRPI0407299A (en) * 2003-02-05 2006-02-07 Gen Hospital Corp Methods for detecting an analyte associated with hiv, for determining the ratio of cd4+ t cells to cd8+ t cells, and for detecting hiv rna in a blood sample
JP4784508B2 (en) * 2004-05-07 2011-10-05 コニカミノルタエムジー株式会社 Inspection microreactor, inspection apparatus, and inspection method
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
DE102005059536B4 (en) * 2005-12-13 2008-08-28 Siemens Ag Method and biochip for studying a biological sample
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
CN101544351B (en) * 2009-05-08 2011-12-14 东南大学 Low-dimensional nanophase material high-flexibility assembling chip and application method
US10954546B2 (en) 2013-09-24 2021-03-23 Ait Austrian Institute Of Technology Gmbh Method for pathogen isolation
WO2015103225A1 (en) * 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
EP3112869A1 (en) * 2015-06-30 2017-01-04 IMEC vzw Sensor device
GB201704761D0 (en) 2017-01-05 2017-05-10 Illumina Inc Common line selector valve for a system
KR102562371B1 (en) * 2021-08-19 2023-08-02 (주)엠큐빅 Microalgae analysis device using artificial intelligence

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661451A (en) * 1984-02-06 1987-04-28 Ortho Diagnostic Systems, Inc. Methods for immobilizing and translocating biological cells
US5795457A (en) * 1990-01-30 1998-08-18 British Technology Group Ltd. Manipulation of solid, semi-solid or liquid materials
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6071394A (en) * 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
GB9615775D0 (en) * 1996-07-26 1996-09-04 British Tech Group Apparatus and method for characterising particles using dielectrophoresis

Also Published As

Publication number Publication date
JP2002536962A (en) 2002-11-05
EP1144092A1 (en) 2001-10-17
AU2595000A (en) 2000-07-12
KR20010102961A (en) 2001-11-17
WO2000037163A1 (en) 2000-06-29
EP1144092A4 (en) 2002-10-29
AU763514B2 (en) 2003-07-24
CA2353461A1 (en) 2000-06-29
NZ512087A (en) 2003-05-30

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 6A E 7A ANUIDADES

B08H Application fees: decision cancelled [chapter 8.8 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1907 DE 24/07/2007 POR TER SIDO INDEVIDO.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1922 DE 06/11/2007.