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BR9913089A - Expressão aumentada da proteìna inseticida cry3b em plantas - Google Patents

Expressão aumentada da proteìna inseticida cry3b em plantas

Info

Publication number
BR9913089A
BR9913089A BR9913089-0A BR9913089A BR9913089A BR 9913089 A BR9913089 A BR 9913089A BR 9913089 A BR9913089 A BR 9913089A BR 9913089 A BR9913089 A BR 9913089A
Authority
BR
Brazil
Prior art keywords
protein
cry3b
plants
higher levels
insecticide
Prior art date
Application number
BR9913089-0A
Other languages
English (en)
Other versions
BRPI9913089B1 (pt
Inventor
Charles P Romano
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Publication of BR9913089A publication Critical patent/BR9913089A/pt
Publication of BRPI9913089B1 publication Critical patent/BRPI9913089B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Patente de Invenção: <B>''EXPRESSãO AUMENTADA DA PROTEìNA INSETICIDA CRY3B EM PLANTAS''<D>. A presente invenção refere-se a processos e composições que compreendem um grupo de novos cassetes de expressão que fornece níveis significativamente aumentados do acúmulo das seq³ências de aminoácidos de Cry3B e de variante de Cry3B inibidoras de Coleópteros quando estas são expressas em plantas. As modalidades preferidas da invenção fornecem níveis pelo menos até dez vezes maiores de proteína controladora de inseto em relação aos níveis mais altos obtidos utilizando as composições anteriores. Em particular, o milho transgênico expressando níveis maiores de uma proteína projetada para exibir toxicidade aumentada para pragas de Coleópteros espalha níveis superiores de proteção contra os insetos e menos provavelmente são responsáveis pelo desenvolvimento de populações de insetos alvo que são resistentes à proteína ativa como inseticida.
BRPI9913089A 1998-08-19 1999-08-19 sequencia de polinucleotídeos recombinante para expressão aumentada da proteína inseticida cry3b em plantas, bem como processo para controle da infestação de insetos coleópteros BRPI9913089B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9715098P 1998-08-19 1998-08-19
PCT/US1999/018883 WO2000011185A2 (en) 1998-08-19 1999-08-19 Improved expression of cry3b insecticidal protein in plants

Publications (2)

Publication Number Publication Date
BR9913089A true BR9913089A (pt) 2001-05-15
BRPI9913089B1 BRPI9913089B1 (pt) 2017-03-07

Family

ID=22261516

Family Applications (2)

Application Number Title Priority Date Filing Date
BRPI9913690A BRPI9913690B1 (pt) 1998-08-19 1999-08-18 molécula de dna recombinante, célula de microorganismo transgênico, e método para prover expressão gênica aprimorada em plantas
BRPI9913089A BRPI9913089B1 (pt) 1998-08-19 1999-08-19 sequencia de polinucleotídeos recombinante para expressão aumentada da proteína inseticida cry3b em plantas, bem como processo para controle da infestação de insetos coleópteros

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BRPI9913690A BRPI9913690B1 (pt) 1998-08-19 1999-08-18 molécula de dna recombinante, célula de microorganismo transgênico, e método para prover expressão gênica aprimorada em plantas

Country Status (17)

Country Link
EP (5) EP2080805B1 (pt)
CN (1) CN100390284C (pt)
AR (2) AR021465A1 (pt)
AT (3) ATE506441T1 (pt)
AU (3) AU5571699A (pt)
BR (2) BRPI9913690B1 (pt)
CA (2) CA2340286C (pt)
CZ (2) CZ301915B6 (pt)
DE (3) DE69943375D1 (pt)
DK (2) DK1121440T3 (pt)
ES (3) ES2365204T3 (pt)
ME (2) MEP19908A (pt)
PL (1) PL346650A1 (pt)
PT (2) PT1121440E (pt)
WO (3) WO2000011200A2 (pt)
YU (1) YU13501A (pt)
ZA (1) ZA200100874B (pt)

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Also Published As

Publication number Publication date
BRPI9913690B1 (pt) 2017-03-21
YU13501A (sh) 2005-06-10
ATE459717T1 (de) 2010-03-15
DE69937459D1 (de) 2007-12-13
WO2000011200A3 (en) 2000-08-31
ATE506441T1 (de) 2011-05-15
AU5578399A (en) 2000-03-14
CA2340324C (en) 2011-01-11
EP1698699B1 (en) 2010-03-03
ME00085B (me) 2010-10-10
DE69937459T2 (de) 2008-07-31
ES2340057T3 (es) 2010-05-28
WO2000011178A2 (en) 2000-03-02
EP1104481B1 (en) 2011-04-20
DK1698699T3 (da) 2010-05-10
EP1104481A2 (en) 2001-06-06
WO2000011185A2 (en) 2000-03-02
WO2000011178A3 (en) 2000-06-02
WO2000011185A3 (en) 2000-06-02
AU5571699A (en) 2000-03-14
CA2340286A1 (en) 2000-03-02
PT1698699E (pt) 2010-03-22
DE69942109D1 (de) 2010-04-15
EP1698699A3 (en) 2006-09-27
EP1121440B1 (en) 2007-10-31
DE69943375D1 (de) 2011-06-01
ATE377080T1 (de) 2007-11-15
CN1334874A (zh) 2002-02-06
EP2811024B1 (en) 2015-11-18
PL346650A1 (en) 2002-02-25
AU767146B2 (en) 2003-10-30
WO2000011200A2 (en) 2000-03-02
EP2811024A1 (en) 2014-12-10
DK1121440T3 (da) 2008-01-28
ZA200100874B (en) 2002-01-31
MEP19908A (en) 2010-06-10
CZ301915B6 (cs) 2010-07-28
AR075146A2 (es) 2011-03-09
EP2080805B1 (en) 2014-10-08
CZ2001597A3 (cs) 2001-08-15
CA2340324A1 (en) 2000-03-02
AR021465A1 (es) 2002-07-24
PT1121440E (pt) 2008-01-15
EP1121440A2 (en) 2001-08-08
AU5571999A (en) 2000-03-14
ES2294857T3 (es) 2008-04-01
CA2340286C (en) 2015-07-14
EP1698699A2 (en) 2006-09-06
BRPI9913089B1 (pt) 2017-03-07
CZ302074B6 (cs) 2010-09-29
EP2080805A1 (en) 2009-07-22
CN100390284C (zh) 2008-05-28
BR9913690A (pt) 2001-06-05
ES2365204T3 (es) 2011-09-26

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