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BR9907997A - Specific pollen promoter - Google Patents

Specific pollen promoter

Info

Publication number
BR9907997A
BR9907997A BR9907997-6A BR9907997A BR9907997A BR 9907997 A BR9907997 A BR 9907997A BR 9907997 A BR9907997 A BR 9907997A BR 9907997 A BR9907997 A BR 9907997A
Authority
BR
Brazil
Prior art keywords
promoter
pollen
nucleic acid
well
transformation
Prior art date
Application number
BR9907997-6A
Other languages
Portuguese (pt)
Inventor
Andrew James Greenland
Hilary Joan Rogers
Patrick Joseph Hussey
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9803660.1A external-priority patent/GB9803660D0/en
Priority claimed from GBGB9803661.9A external-priority patent/GB9803661D0/en
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Publication of BR9907997A publication Critical patent/BR9907997A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"PROMOTOR ESPECìFICO DE PóLEN" Um ácido nucléico recombinante que compreende uma seq³ência promotora do gene ZmC5 em milho, ou uma sua variante ou fragmento, que atua como como um promotor em pólen. A seq³ência de DNA do promotor é mostrada na Figura 5. Cassetes de expressão e sistemas de expressão assim como processos de transformação, plantas transformadas incluindo a seq³ência promotora da invenção também são reivindicados. O ácido nucléico pode ser usado inter alia na produção de plantas estéreis machos e/ou híbridos assim como na transformação de pólen."PóLEN-SPECIFIC PROMOTOR" A recombinant nucleic acid comprising a promoter sequence of the ZmC5 gene in maize, or a variant or fragment thereof, which acts as a pollen promoter. The DNA sequence of the promoter is shown in Figure 5. Expression cassettes and expression systems as well as transformation processes, transformed plants including the promoter sequence of the invention are also claimed. Nucleic acid can be used inter alia in the production of sterile male and / or hybrid plants as well as in the transformation of pollen.

BR9907997-6A 1998-02-20 1999-01-22 Specific pollen promoter BR9907997A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9803660.1A GB9803660D0 (en) 1998-02-20 1998-02-20 Expression system
GBGB9803661.9A GB9803661D0 (en) 1998-02-20 1998-02-20 Pollen specific promoter
PCT/GB1999/000232 WO1999042587A1 (en) 1998-02-20 1999-01-22 Pollen specific promoter

Publications (1)

Publication Number Publication Date
BR9907997A true BR9907997A (en) 2000-10-24

Family

ID=26313163

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9907997-6A BR9907997A (en) 1998-02-20 1999-01-22 Specific pollen promoter

Country Status (11)

Country Link
EP (1) EP1054970A1 (en)
JP (1) JP2002504335A (en)
KR (1) KR20010041129A (en)
CN (1) CN1301300A (en)
AU (1) AU751438B2 (en)
BR (1) BR9907997A (en)
CA (1) CA2319079A1 (en)
DZ (1) DZ2725A1 (en)
HU (1) HUP0100787A3 (en)
IL (1) IL137971A0 (en)
WO (1) WO1999042587A1 (en)

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CN102533761B (en) * 2011-12-12 2013-03-20 华中科技大学 Pollen-specific promoter and expression vector and application thereof
BR102013002599B1 (en) 2012-02-01 2022-04-05 Dow Agrosciences Llc Isolated or recombinant nucleic acid molecule, method of generating a glyphosate-tolerant plant cell and uses thereof
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MX2014011043A (en) 2012-03-13 2015-06-02 Pioneer Hi Bred Int Genetic reduction of male fertility in plants.
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Also Published As

Publication number Publication date
CA2319079A1 (en) 1999-08-26
WO1999042587A1 (en) 1999-08-26
HUP0100787A2 (en) 2002-04-29
EP1054970A1 (en) 2000-11-29
AU2287699A (en) 1999-09-06
DZ2725A1 (en) 2003-09-01
IL137971A0 (en) 2001-10-31
HUP0100787A3 (en) 2003-04-28
CN1301300A (en) 2001-06-27
AU751438B2 (en) 2002-08-15
JP2002504335A (en) 2002-02-12
KR20010041129A (en) 2001-05-15

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