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BR9714398A - Methods of using the nim1 gene to confer disease resistance in plants - Google Patents

Methods of using the nim1 gene to confer disease resistance in plants

Info

Publication number
BR9714398A
BR9714398A BR9714398-7A BR9714398A BR9714398A BR 9714398 A BR9714398 A BR 9714398A BR 9714398 A BR9714398 A BR 9714398A BR 9714398 A BR9714398 A BR 9714398A
Authority
BR
Brazil
Prior art keywords
plants
nim1
gene
resistance
altered forms
Prior art date
Application number
BR9714398-7A
Other languages
Portuguese (pt)
Inventor
John Andrew Ryals
Kay Ann Lawton
Scott Joseph Uknes
Henry York Steiner
Michelle Denise Hunt
Leslie Bethards Friedrich
Original Assignee
Novatrtis Ag Novartis Sa Novar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/880,179 external-priority patent/US6091004A/en
Application filed by Novatrtis Ag Novartis Sa Novar filed Critical Novatrtis Ag Novartis Sa Novar
Publication of BR9714398A publication Critical patent/BR9714398A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Dentistry (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Hydroponics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Patente de Invenção: <B>"MéTODOS DE UTILIZAR O GENE NIM1 PARA CONFERIR RESISTêNCIA A DOENçAS EM PLANTAS"<D>. A invenção diz respeito à posição e à caracterização de um gene (designado NIM1), que é um componente-chave do caminho da SAR e que em conexão com os indutores químicos e biológicos, capacita a indução da expressão do gene para SAR e da resistência a doenças de amplo espectro em plantas. O produto de gene NIM1 é um homólogo estrutural da subclasse ( do fato (B de transdução de sinal de mamíferos. A presente invenção explora esta descoberta para proporcionar formas alteradas de NIM1, que atuam como reguladores negativos dominantes do caminho de transdução de sinal da resistência adquirida sistêmica (SAR). Estas formas alteradas de NIM1 conferem o fenótipo oposto que o mutante de nim1 em plantas transformadas com as formas alteradas de NIM1, i.e., as plantas transgênicas exibem expressão do gene para SAR constitutiva e um fenótipo de imunidade constitutiva (CIM). A invenção adicionalmente diz respeito aos vetores de transformação e aos processos para a superexpressão do gene NIM1 em plantas. As plantas transgênicas assim criadas têm resistência a doenças de amplo espectro. A presente invenção adicionalmente diz respeito às moléculas de DNA codificando as formas alteradas do gene NIM1, aos vetores de expressão contendo tais moléculas de DNA, e às plantas e às células vegetais transformadas com o mesmo. A invenção adicionalmente refere-se ao vetores de transformação e aos processos para a superexpressão do gene NIM1 em plantas. São divulgados os vetores e os processos para a produção da superexpressão do gene NIM1 em plantas. A invenção também refere-se aos métodos de ativar a SAR em plantas e conferir, às plantas, um fenótipo de CIM e resistência a doenças de amplo espectro, por transformação das plantas com moléculas de DNA codificando as formas alteradas do produto de gene NIM1.Invention Patent: <B> "METHODS OF USING THE NIM1 GENE TO CONFIRM RESISTANCE TO PLANT DISEASES" <D>. The invention concerns the position and characterization of a gene (called NIM1), which is a key component of the SAR pathway and which, in connection with chemical and biological inducers, enables the induction of SAR gene expression and resistance broad-spectrum diseases in plants. The NIM1 gene product is a structural homologue of the subclass (of the fact (B of mammalian signal transduction. The present invention exploits this discovery to provide altered forms of NIM1, which act as dominant negative regulators of the resistance signal transduction pathway These altered forms of NIM1 confer the opposite phenotype to the nim1 mutant in plants transformed with the altered forms of NIM1, ie, transgenic plants exhibit expression of the constitutive SAR gene and a constitutive immunity phenotype (MIC) The invention additionally concerns transformation vectors and processes for the overexpression of the NIM1 gene in plants. The transgenic plants thus created have resistance to broad spectrum diseases. The present invention additionally concerns the DNA molecules encoding the altered forms. of the NIM1 gene, to the expression vectors containing such DNA molecules, and to plants and plant cells transformed with it. The invention additionally relates to transformation vectors and processes for overexpression of the NIM1 gene in plants. Vectors and processes for producing overexpression of the NIM1 gene in plants are disclosed. The invention also relates to methods of activating SAR in plants and giving plants a MIC phenotype and resistance to broad spectrum diseases by transforming plants with DNA molecules encoding the altered forms of the NIM1 gene product.

BR9714398-7A 1996-12-13 1997-12-12 Methods of using the nim1 gene to confer disease resistance in plants BR9714398A (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US3317796P 1996-12-13 1996-12-13
US3438296P 1996-12-27 1996-12-27
US3437996P 1996-12-27 1996-12-27
US3502297P 1997-01-10 1997-01-10
US3473097P 1997-01-10 1997-01-10
US3502197P 1997-01-10 1997-01-10
US08/880,179 US6091004A (en) 1996-06-21 1997-06-20 Gene encoding a protein involved in the signal transduction cascade leading to systemic acquired resistance in plants
PCT/EP1997/007012 WO1998026082A1 (en) 1996-12-13 1997-12-12 Methods of using the nim1 gene to confer disease resistance in plants

Publications (1)

Publication Number Publication Date
BR9714398A true BR9714398A (en) 2000-05-02

Family

ID=27567813

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9714398-7A BR9714398A (en) 1996-12-13 1997-12-12 Methods of using the nim1 gene to confer disease resistance in plants

Country Status (9)

Country Link
EP (1) EP0944728A1 (en)
JP (1) JP2001505774A (en)
AU (1) AU727179B2 (en)
BR (1) BR9714398A (en)
CA (1) CA2273189A1 (en)
FR (1) FR2757875A1 (en)
IT (1) IT1298472B1 (en)
NL (1) NL1007779C2 (en)
WO (1) WO1998026082A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL331535A1 (en) * 1996-08-09 1999-07-19 Gen Hospital Corp Acquired immunity genes and their application
AR010855A1 (en) * 1996-12-27 2000-07-12 Syngenta Participations Ag METHOD FOR PLANT PROTECTION
EP1012316A1 (en) 1997-09-15 2000-06-28 Institute of Molecular Agrobiology Rank1, an ankyrin-repeat containing peptide from rice associated with disease resistance
CN1355847A (en) * 1999-03-09 2002-06-26 辛根塔参与股份公司 Novel plant genes and their uses
US6528702B1 (en) 1999-03-09 2003-03-04 Syngenta Participations Ag Plant genes and uses thereof
US6504084B1 (en) 1999-04-23 2003-01-07 Pioneer Hi-Bred International, Inc. Maize NPR1 polynucleotides and methods of use
CA2372654A1 (en) 1999-05-13 2000-11-23 Monsanto Technology Llc Acquired resistance genes in plants
US7199286B2 (en) 1999-12-15 2007-04-03 Syngenta Participations Ag Plant-derived novel pathogen and SAR-induction chemical induced promoters, and fragments thereof
US6706952B1 (en) 1999-12-15 2004-03-16 Syngenta Participations Ag Arabidopsis gene encoding a protein involved in the regulation of SAR gene expression in plants
AU2279601A (en) * 1999-12-21 2001-07-03 Pioneer Hi-Bred International, Inc. Npr1-interactors and methods of use
WO2003057593A1 (en) 2001-12-21 2003-07-17 Nektar Therapeutics Capsule package with moisture barrier
WO2006081301A2 (en) 2005-01-26 2006-08-03 Washington State University Research Foundation Plant defense signal peptides
US20150353949A1 (en) 2012-01-11 2015-12-10 The Australian National University Method for modulating plant root architecture
BR112016008172B1 (en) 2013-10-16 2021-06-15 The Australian National University METHOD FOR MODULATION OF PLANT GROWTH
CN115011567A (en) * 2022-06-24 2022-09-06 安徽农业大学 (+) -neomenthol synthase and synthase gene and application thereof in tea tree disease resistance

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016077A1 (en) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Method for breeding disease resistance into plants
EP0923648A1 (en) * 1996-06-21 1999-06-23 Novartis AG Gene conferring disease resistance in plants and uses thereof

Also Published As

Publication number Publication date
JP2001505774A (en) 2001-05-08
AU727179B2 (en) 2000-12-07
ITMI972741A1 (en) 1999-06-11
IT1298472B1 (en) 2000-01-10
FR2757875A1 (en) 1998-07-03
WO1998026082A1 (en) 1998-06-18
CA2273189A1 (en) 1998-06-18
NL1007779A1 (en) 1998-06-17
AU5663198A (en) 1998-07-03
NL1007779C2 (en) 1998-07-22
EP0944728A1 (en) 1999-09-29

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 8A E 9A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1903 DE 26/06/2007.