[go: up one dir, main page]

BR9709733A - Construção de dna quimérico recominante e processo para seleção de linhagens - Google Patents

Construção de dna quimérico recominante e processo para seleção de linhagens

Info

Publication number
BR9709733A
BR9709733A BR9709733A BR9709733A BR9709733A BR 9709733 A BR9709733 A BR 9709733A BR 9709733 A BR9709733 A BR 9709733A BR 9709733 A BR9709733 A BR 9709733A BR 9709733 A BR9709733 A BR 9709733A
Authority
BR
Brazil
Prior art keywords
strains
selection
construction
chimeric dna
recombinant chimeric
Prior art date
Application number
BR9709733A
Other languages
English (en)
Inventor
Pal Maliga
Helaine Carrer
Sumita Chaudhuri
Original Assignee
Univ Rutgers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers filed Critical Univ Rutgers
Publication of BR9709733A publication Critical patent/BR9709733A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214Plastid transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR9709733A 1996-06-14 1997-06-13 Construção de dna quimérico recominante e processo para seleção de linhagens BR9709733A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1974196P 1996-06-14 1996-06-14
PCT/US1997/010318 WO1997047771A1 (en) 1996-06-14 1997-06-13 Editing-based selectable plastid marker genes

Publications (1)

Publication Number Publication Date
BR9709733A true BR9709733A (pt) 1999-08-10

Family

ID=21794788

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9709733A BR9709733A (pt) 1996-06-14 1997-06-13 Construção de dna quimérico recominante e processo para seleção de linhagens

Country Status (8)

Country Link
US (1) US6297054B1 (pt)
EP (1) EP0960209B1 (pt)
JP (1) JP2000503858A (pt)
CN (1) CN1231702A (pt)
AU (1) AU724582B2 (pt)
BR (1) BR9709733A (pt)
DE (1) DE69738715D1 (pt)
WO (1) WO1997047771A1 (pt)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE414417T1 (de) * 1998-08-03 2008-12-15 Univ Rutgers Translations-kontrollelemente für proteinexpression mit hoher ausbeute in den plastiden von höheren pflanzen und verfahren zu deren verwendung
US7217860B1 (en) 1999-09-21 2007-05-15 Rutgers, The State University Of New Jersey Site-specific recombination system to manipulate the plastid genome of higher plants
US7186560B2 (en) * 1999-09-21 2007-03-06 Rutgers, The State University Of New Jersey High level expression of immunogenic proteins in the plastids of higher plants
CN100480376C (zh) 2000-03-22 2009-04-22 依康遗传学股份有限公司 转化植物质体和制备转质体基因组植物的方法
US20050198704A1 (en) * 2000-10-20 2005-09-08 Board Of Trustees Of Michigan State University Chloroplast transgenesis of monocots: bioconfined genetically engineered monocot crops that will eliminate transgene flow
AU2002252525A1 (en) * 2001-03-29 2002-10-15 Rutgers, The University Of New Jersey Integrases for the insertion of heterologous nucleic acids into the plastid genome
US7176355B2 (en) 2002-12-13 2007-02-13 Rutgers, The State University Of New Jersey Plastid rRNA operon promoter elements for construction of chimeric promoters for transgene expression
EP1606386A4 (en) * 2003-03-03 2007-07-18 Univ Rutgers REMOVAL OF PLASTIC SEQUENCES THROUGH TRANSIENT EXPRESSED LOCATION-SPECIFIC RECOMBINATION
WO2005059146A1 (en) * 2003-12-15 2005-06-30 Vib Vzw Avehicle for plant transformation
WO2007121467A2 (en) * 2006-04-18 2007-10-25 Rutgers, The State University Of New Jersey Compositions and methods for increasing transgene expression in the plastids of higher plants
US7745698B2 (en) * 2006-11-16 2010-06-29 Wisconsin Alumni Research Foundation Peptide extension for enhancement of transgene expression in chloroplasts
SI2147105T1 (sl) 2007-05-02 2013-08-30 Merial Limited DNA-plazmidi z izboljšano ekspresijo in stabilnostjo
CN107177606A (zh) * 2007-05-09 2017-09-19 美国陶氏益农公司 新除草剂抗性基因
KR20130020842A (ko) * 2007-06-01 2013-02-28 더 스크립스 리서치 인스티튜트 유전적으로 변형된 광합성 생물들의 고처리량 스크리닝
EP2188376A4 (en) * 2007-09-11 2013-07-10 Sapphire Energy Inc MOLECULAR PRODUCTION BY PHOTOSYNTHETIC ORGANISMS
US9695372B2 (en) * 2007-09-11 2017-07-04 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms
EP2297326A4 (en) 2008-06-06 2011-11-16 Aurora Biofuels Inc VCP-BASED VECTORS FOR THE TRANSFORMATION OF ALGAE CELLS
US8314228B2 (en) 2009-02-13 2012-11-20 Aurora Algae, Inc. Bidirectional promoters in Nannochloropsis
US9029137B2 (en) 2009-06-08 2015-05-12 Aurora Algae, Inc. ACP promoter
US8809046B2 (en) 2011-04-28 2014-08-19 Aurora Algae, Inc. Algal elongases
US8865468B2 (en) * 2009-10-19 2014-10-21 Aurora Algae, Inc. Homologous recombination in an algal nuclear genome
US8709765B2 (en) * 2009-07-20 2014-04-29 Aurora Algae, Inc. Manipulation of an alternative respiratory pathway in photo-autotrophs
US8722359B2 (en) 2011-01-21 2014-05-13 Aurora Algae, Inc. Genes for enhanced lipid metabolism for accumulation of lipids
WO2012149457A2 (en) 2011-04-28 2012-11-01 Aurora Algae, Inc. Algal desaturases
FR3105977B1 (fr) * 2020-01-07 2022-02-11 Woodlight Méthode de fabrication de plantes bioluminescentes
CN112106635A (zh) * 2020-09-22 2020-12-22 云南中烟工业有限责任公司 一种利用卡那霉素快速筛选无标签基因编辑烟草素材种子的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants

Also Published As

Publication number Publication date
AU724582B2 (en) 2000-09-28
DE69738715D1 (de) 2008-07-03
AU3392897A (en) 1998-01-07
EP0960209A1 (en) 1999-12-01
EP0960209A4 (en) 2003-04-16
EP0960209B1 (en) 2008-05-21
JP2000503858A (ja) 2000-04-04
WO1997047771A1 (en) 1997-12-18
US6297054B1 (en) 2001-10-02
CN1231702A (zh) 1999-10-13

Similar Documents

Publication Publication Date Title
BR9709733A (pt) Construção de dna quimérico recominante e processo para seleção de linhagens
BR9710124A (pt) Processo para preparar um óxido de olefina processo para preparar óxido de propoleno e composição
NO960408D0 (no) DNA sekvenseringsenzymer
ID22852A (id) Bahan-bahan biokontrol antifungi, proses untuk membuatnya dan memperlakukannya
BR9509904A (pt) Processo para purificação e isolamento de 2-desóxi-2,2-difluor-nucleosideos
AU8264898A (en) Human jagged polypeptide, encoding nucleic acids and methods of use
EE9900357A (et) Geenisekvenaator ja meetodid
BR9608647A (pt) Amilase dna e composição de detergente
FI970820A7 (fi) Jäänmurtamismenetelmä ja jäänmurtaja
PT917879E (pt) Processo melhorado para estabilizacao de proteinas
EP0948651A4 (en) RECOMBINANT N-SMASES AND CODING NUCLEIC ACIDS
PT940381E (pt) Processo para recuperacao de ciclopentano e/ou ciclopenteno
PL327536A1 (en) Method of obtaining paraffins or their fractions
FI953640L (fi) Termostabiilit ksylanaasi-DNA, proteiini ja käyttömenetelmät
NO971448D0 (no) Anti-Fas rekombinante antistoffer og DNA derfor
EP1155117A4 (en) SECRETED PROTEINS AND NUCLEIC ACIDS ENCODING THESE PROTEINS
ID24077A (id) Material dan proses pembersihan saluran
CY2005003I1 (el) Μεθοδοι και ενδιαμεσα χρησιμα για κατασκευη αντιφολικων
BR9700896A (pt) Dispositivo de suspensão para obras de engenharia civil e processo de construção
BR9804794A (pt) Processo para prepara-Æo de 1-alquil-4-(2-cloro-3-alcÄxi-4-alquil-sulfonil-benzoil) -5-hidrÄxi-pirazol e compostos relacioonados
BR9800610A (pt) Processo de isomerização
BR9606027A (pt) Processo para cozimento ou calcinação de corpos de carbono e material de compactação
AU4245096A (en) Biologically active fragments of thermus flavus dna polymerase
BR9701207A (pt) Processo para preparação de indenos
BR9601017A (pt) Processo para a preparação de ciclosporina A e respectiva ciclosporina A

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: O DEPOSITANTE DEIXOU DE APRESENTAR MANIFESTACAO SOBRE O PARECER NEGATIVO, ASSIM, DE ACORDO COM O ART. 36 DA LPI (LEI 9279/96) 2O, O PEDIDO VOLTA A EXAME SENDO CONSIDERADO NAO PATENTEAVEL PELAS RAZOES EXPOSTAS NO PARECER TECNICO ANTERIOR.