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BR112019005748A2 - affinity-conjugates of oligonucleotides and uses of these - Google Patents

affinity-conjugates of oligonucleotides and uses of these

Info

Publication number
BR112019005748A2
BR112019005748A2 BR112019005748A BR112019005748A BR112019005748A2 BR 112019005748 A2 BR112019005748 A2 BR 112019005748A2 BR 112019005748 A BR112019005748 A BR 112019005748A BR 112019005748 A BR112019005748 A BR 112019005748A BR 112019005748 A2 BR112019005748 A2 BR 112019005748A2
Authority
BR
Brazil
Prior art keywords
conjugates
affinity
oligonucleotides
compositions
methods
Prior art date
Application number
BR112019005748A
Other languages
Portuguese (pt)
Inventor
J Belmont Brian
Vigneault Francois
J Goldfless Stephen
Adrian Briggs Wrangham
Original Assignee
Abvitro Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2016/053598 external-priority patent/WO2017053905A1/en
Application filed by Abvitro Llc filed Critical Abvitro Llc
Publication of BR112019005748A2 publication Critical patent/BR112019005748A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

são aqui proporcionados métodos e composições para caracterização de célula única usando afinidade-conjugados de oligonucleotídeo. são aqui proporcionados métodos e composições para caracterização de célula única usando tetrâmero-conjugados de oligonucleotídeo.Methods and compositions for single cell characterization using affinity-oligonucleotide conjugates are provided herein. Methods and compositions for single cell characterization using oligonucleotide tetramer-conjugates are provided herein.

BR112019005748A 2016-09-24 2017-01-12 affinity-conjugates of oligonucleotides and uses of these BR112019005748A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2016/053598 WO2017053905A1 (en) 2015-09-24 2016-09-24 Affinity-oligonucleotide conjugates and uses thereof
PCT/US2017/013274 WO2018057051A1 (en) 2016-09-24 2017-01-12 Affinity-oligonucleotide conjugates and uses thereof

Publications (1)

Publication Number Publication Date
BR112019005748A2 true BR112019005748A2 (en) 2019-06-18

Family

ID=57960826

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019005748A BR112019005748A2 (en) 2016-09-24 2017-01-12 affinity-conjugates of oligonucleotides and uses of these

Country Status (5)

Country Link
JP (1) JP6929354B2 (en)
AU (1) AU2017332495A1 (en)
BR (1) BR112019005748A2 (en)
IL (1) IL265471A (en)
WO (1) WO2018057051A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12258613B2 (en) * 2017-03-08 2025-03-25 California Institute Of Technology Pairing antigen specificity of a T cell with T cell receptor sequences
US20230183791A1 (en) 2017-03-24 2023-06-15 National University Of Singapore Methods For Multiplex Detection Of Molecules
EP3631012B1 (en) 2017-05-26 2022-06-08 AbVitro LLC High-throughput polynucleotide library sequencing and transcriptome analysis
CN112005115A (en) 2018-02-12 2020-11-27 10X基因组学有限公司 Methods to characterize multiple analytes from single cells or cell populations
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
JP7145980B2 (en) * 2018-06-15 2022-10-03 エフ．ホフマン－ラ ロシュ アーゲー System for identifying antigens recognized by T-cell receptors expressed on tumor-infiltrating lymphocytes
JP2022502029A (en) * 2018-09-24 2022-01-11 ブリストル−マイヤーズ スクイブ カンパニーBristol−Myers Squibb Company Barcoded peptide-MHC complex and its use
GB201816522D0 (en) * 2018-10-10 2018-11-28 Autolus Ltd Methods and reagents for analysing nucleic acids from single cells
MX2021007292A (en) * 2018-12-18 2021-09-21 Mbl Int Corp COMPOSITIONS OF STREPTAVIDIN-OLIGO CONJUGATES OF VARYING pMHC OCCUPANCY.
JP6821230B2 (en) * 2019-02-04 2021-01-27 国立大学法人愛媛大学 How to make CAR library and scFv
US12416037B2 (en) * 2020-01-24 2025-09-16 Vanderbilt University Methods for identification of ligand-blocking antibodies and for determining antibody potency
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
WO2021251867A1 (en) * 2020-06-09 2021-12-16 Simsen Diagnostics Ab, Lymphocyte clonality determination

Family Cites Families (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US5019384A (en) 1986-06-30 1991-05-28 Massachusetts Institute Of Technology Immunonodulating compositions and their use
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
EP0428534B1 (en) 1988-06-14 1995-03-29 Cetus Oncology Corporation Coupling agents and sterically hindered disulfide linked conjugates prepared therefrom
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
DE3920358A1 (en) 1989-06-22 1991-01-17 Behringwerke Ag BISPECIFIC AND OLIGO-SPECIFIC, MONO- AND OLIGOVALENT ANTI-BODY CONSTRUCTS, THEIR PRODUCTION AND USE
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5733523A (en) 1990-12-10 1998-03-31 Akzo Nobel N.V. Targeted delivery of a therapeutic entity using complementary oligonucleotides
JP3266311B2 (en) 1991-05-02 2002-03-18 生化学工業株式会社 Novel polypeptide and anti-HIV agent using the same
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
DE69233087T2 (en) 1991-11-22 2003-12-24 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Process for the production of polymer arrays
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
JPH07501451A (en) 1991-11-25 1995-02-16 エンゾン・インコーポレイテッド Multivalent antigen binding protein
EP0746609A4 (en) 1991-12-17 1997-12-17 Genpharm Int Transgenic non-human animals capable of producing heterologous antibodies
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
EP0758403B1 (en) 1994-05-05 1998-06-24 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
SE504798C2 (en) 1995-06-16 1997-04-28 Ulf Landegren Immunoassay and test kits with two reagents that can be cross-linked if adsorbed to the analyte
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US5981956A (en) 1996-05-16 1999-11-09 Affymetrix, Inc. Systems and methods for detection of labeled materials
JP4468488B2 (en) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド Detection of nucleic acid sequence differences using combined ligase detection and polymerase chain reaction
US6174670B1 (en) 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6620587B1 (en) 1997-05-28 2003-09-16 Discerna Limited Ribosome complexes as selection particles for in vitro display and evolution of proteins
JP2001521753A (en) 1997-10-31 2001-11-13 アフィメトリックス インコーポレイテッド Expression profiles in adult and fetal organs
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO1999057321A1 (en) 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
CA2366459A1 (en) 1999-03-26 2000-10-05 Affymetrix, Inc. Universal arrays
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
EP1421115B1 (en) 2001-08-31 2005-03-02 Avidex Limited Soluble t cell receptor
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
NZ539225A (en) 2002-10-09 2006-09-29 Avidex Ltd Single chain recombinant T cell receptors
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
EP2127736A1 (en) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Formation and control of fluidic species
BRPI0414004A (en) 2003-08-27 2006-10-24 Harvard College electronic control of fluidic species
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7643818B2 (en) 2004-11-22 2010-01-05 Seven Networks, Inc. E-mail messaging to/from a mobile terminal
EP2248578B1 (en) 2005-03-04 2012-06-06 President and Fellows of Harvard College Method for forming multiple emulsions
US20100227770A1 (en) 2005-09-13 2010-09-09 Randall True Brownian microbarcodes for bioassays
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
AU2007210152A1 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
EP4190448A3 (en) 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Microfluidic devices
CN102083575B (en) 2007-06-25 2014-09-17 阿费梅特里克斯公司 imprinted microcode
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
CN102405402A (en) 2008-09-23 2012-04-04 阔达生命有限公司 Droplet-based assay system
US10464987B2 (en) 2009-10-06 2019-11-05 Abbvie Inc. Human single-chain T cell receptors
WO2014145047A1 (en) * 2013-03-15 2014-09-18 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
EP3152232B1 (en) * 2014-06-06 2019-11-13 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
WO2016044227A1 (en) * 2014-09-15 2016-03-24 Abvitro, Inc. High-throughput nucleotide library sequencing
EP4636401A2 (en) * 2014-12-19 2025-10-22 The Broad Institute, Inc. Methods for profiling the t-cell-receptor repertoire

Also Published As

Publication number Publication date
JP2019537430A (en) 2019-12-26
WO2018057051A1 (en) 2018-03-29
AU2017332495A1 (en) 2019-04-11
JP6929354B2 (en) 2021-09-01
IL265471A (en) 2019-05-30

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Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements