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BR102022018204A2 - MICROENCAPSULATION OF ORANGE JUICE (CITRUS SINENSIS) AND HYDROLYZED COLLAGEN BY IONIC GELIFICATION - Google Patents

MICROENCAPSULATION OF ORANGE JUICE (CITRUS SINENSIS) AND HYDROLYZED COLLAGEN BY IONIC GELIFICATION Download PDF

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Publication number
BR102022018204A2
BR102022018204A2 BR102022018204-3A BR102022018204A BR102022018204A2 BR 102022018204 A2 BR102022018204 A2 BR 102022018204A2 BR 102022018204 A BR102022018204 A BR 102022018204A BR 102022018204 A2 BR102022018204 A2 BR 102022018204A2
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BR
Brazil
Prior art keywords
orange juice
hydrolyzed collagen
collagen
solution
microencapsulation
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BR102022018204-3A
Other languages
Portuguese (pt)
Inventor
Alessandra Almeida Castro Pagani
Patricia Nogueira Matos
Celestina Tojal Machado
Aline Andrade Reis
Jucenir Dos Santos
Original Assignee
Universidade Federal De Sergipe
Filing date
Publication date
Application filed by Universidade Federal De Sergipe filed Critical Universidade Federal De Sergipe
Publication of BR102022018204A2 publication Critical patent/BR102022018204A2/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/385Concentrates of non-alcoholic beverages
    • A23L2/39Dry compositions
    • A23L2/395Dry compositions in a particular shape or form
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/275Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
    • A23L29/281Proteins, e.g. gelatin or collagen
    • A23L29/284Gelatin; Collagen
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/30Encapsulation of particles, e.g. foodstuff additives

Abstract

A presente invenção refere-se ao processo de obtenção de microcápsulas de suco de laranja e colágeno hidrolisado, sendo produzidas por meio do processo de gelificação iônica, a fim de que possam ser aplicadas em alimentos como uma boa fonte de colágeno e vitamina C. As microcápsulas podem ser consumidas como complemento em outros alimentos ou até mesmo, de forma pura. As microcápsulas produzidas foram acondicionadas em potes de polipropileno e armazenadas sob refrigeração a 5 ± 2°C por 16 dias, avaliando assim, a sua vida de prateleira.The present invention refers to the process of obtaining microcapsules of orange juice and hydrolyzed collagen, being produced through the ionic gelation process, so that they can be applied to foods as a good source of collagen and vitamin C. microcapsules can be consumed as a complement to other foods or even in pure form. The microcapsules produced were placed in polypropylene jars and stored under refrigeration at 5 ± 2°C for 16 days, thus evaluating their shelf life.

Description

Campo da invençãoField of invention

[001] A presente invenção refere-se ao processo de obtenção de microcápsulas de suco de laranja e colágeno hidrolisado, produzidas pelo processo de gelificação iônica adequada para o uso em produtos alimentícios, podendo ser consumidas individualmente ou incorporadas em outros alimentos como uma boa fonte de colágeno e vitamina C.[001] The present invention refers to the process of obtaining microcapsules of orange juice and hydrolyzed collagen, produced by the ionic gelation process suitable for use in food products, which can be consumed individually or incorporated into other foods as a good source collagen and vitamin C.

Fundamentos da invençãoFundamentals of the invention

[002] A ingestão de compostos antioxidantes e vitamina C está associada à prevenção de diversas doenças relacionadas ao estresse oxidativo humano, como câncer, inflamação, aterosclerose, diabetes, doenças cardiovasculares e neurológicas (CARO, A.D; PIGA, A; VACCA, V; AGABBIO, M. Changes of flavonoids, vitamin C and antioxidant capacity in minimally processed citrus segments and juices during storage. Food Chemistry, vol. 84, n. 1, p. 99-105, 2004). Além disso, a Vitamina C exerce outras atividades biológicas essenciais, auxilia na síntese de colágeno, neurotransmissores, hormônios esteróides e carnitina. Infelizmente, o ser humano não é capaz de sintetizá- la, sendo imprescindível a ingestão de alimentos contendo vitamina C (BARROS, H.R.M; FERREIRA, T.A.P.C; GENOVESE, M.I. Antioxidant capacity and mineral content of pulp and peel from commercial cultivars of citrus from Brazil. Food Chemistry, vol. 134, n. 4, p. 1892-1898, 2012).[002] The intake of antioxidant compounds and vitamin C is associated with the prevention of several diseases related to human oxidative stress, such as cancer, inflammation, atherosclerosis, diabetes, cardiovascular and neurological diseases (CARO, A.D; PIGA, A; VACCA, V; AGABBIO, M. Changes of flavonoids, vitamin C and antioxidant capacity in minimally processed citrus segments and juices during storage. Furthermore, Vitamin C exerts other essential biological activities, assisting in the synthesis of collagen, neurotransmitters, steroid hormones and carnitine. Unfortunately, humans are not capable of synthesizing it, making it essential to eat foods containing vitamin C (BARROS, H.R.M; FERREIRA, T.A.P.C; GENOVESE, M.I. Antioxidant capacity and mineral content of pulp and peel from commercial cultivars of citrus from Brazil . Food Chemistry, vol. 134, no. 4, p.

[003] O colágeno é uma proteína de origem animal que atua principalmente, na integridade estrutural dos tecidos em que está presente. Existem pelo menos 27 tipos de colágeno e são denominados tipo I-XXVII. Ele integra parte da constituição de diversos tecidos fibrosos, como pele, ossos, vasos sanguíneos, tendões, músculos, córnea, ligamentos e dentes (SILVA, T.F. DA; PENNA, A.L.B. Colágeno: caracteristicas químicas e propriedades funcionais. Revista do Instituto Adolfo Lutz, v. 71, n. 3, p. 530-539, 2012).[003] Collagen is a protein of animal origin that acts mainly on the structural integrity of the tissues in which it is present. There are at least 27 types of collagen and they are called type I-XXVII. It is part of the constitution of various fibrous tissues, such as skin, bones, blood vessels, tendons, muscles, cornea, ligaments and teeth (SILVA, T.F. DA; PENNA, A.L.B. Collagen: chemical characteristics and functional properties. Revista do Instituto Adolfo Lutz, v. 71, n. 3, p. 530-539, 2012).

[004] O hidrolisado de colágeno é obtido por hidrólise ácida, básica ou enzimática do colágeno. Devido à melhor biodisponibilidade, os peptídeos com menores pesos moleculares são os favoritos quando comparados com as proteínas originais e peptídeos com maiores pesos moleculares (YAMAMOTO, S; DEGUCHI, K; ONUMA, M; NUMATA, M; SAKAI, Y. Absorption and urinary excretion of peptides after collagen tripeptide ingestion in humans. Biol. Pharm. Bull, vol. 39, p. 428-434, 2016). O uso de peptídeos de colágeno como suplementos alimentares em alimentos e bebidas, e como componentes bioativos em cosméticos, serviços de saúde e produtos farmacêuticos tem expandido (HONG, H; FAN, H; CHALAMAIAH, M; WU, J. Preparation of low-molecular- weight, collagen hydrolysates (peptides): Current progress, challenges, and future perspectives. Food Chemistry, vol. 301, 2019).[004] Collagen hydrolyzate is obtained by acid, basic or enzymatic hydrolysis of collagen. Due to better bioavailability, peptides with lower molecular weights are favorites when compared to the original proteins and peptides with higher molecular weights (YAMAMOTO, S; DEGUCHI, K; ONUMA, M; NUMATA, M; SAKAI, Y. Absorption and urinary excretion of peptides after collagen tripeptide ingestion in humans. The use of collagen peptides as dietary supplements in foods and beverages, and as bioactive components in cosmetics, healthcare services and pharmaceuticals has expanded (HONG, H; FAN, H; CHALAMAIAH, M; WU, J. Preparation of low- molecular weight, collagen hydrolysates (peptides): Current progress, challenges, and future perspectives Food Chemistry, vol.

[005] A encapsulação é uma tecnologia que envolve materiais sólidos, líquidos e gasosos por meio de coberturas poliméricas finas formando uma micropartícula, levando em consideração o baixo custo; fácil execução e não uso de solventes orgânicos, a microencapsulação por gelificação iônica apresenta uma tecnologia bastante aplicável na área de alimentos (Dordevic, V; Balanc, B; Belscak-Cvitanovic, A; Levic, S; Trifkovic, K; Kalusevic, A; Kostic, I; Komes, D; Bugarski, B; Nedovic, V. Encapsulation technology trends for the delivery of bioactive food compounds. Food Engineering Reviews, vol. 7, n. 4, p. 452 - 490, 2015).[005] Encapsulation is a technology that involves solid, liquid and gaseous materials through thin polymeric coatings forming a microparticle, taking into account low cost; easy execution and no use of organic solvents, microencapsulation by ionic gelation presents a technology that is very applicable in the food sector (Dordevic, V; Balanc, B; Belscak-Cvitanovic, A; Levic, S; Trifkovic, K; Kalusevic, A; Kostic, I; Bugarski, B; Nedovic, V. Encapsulation technology trends for the delivery of bioactive food compounds, vol.

[006] A presente invenção se refere em particular a microcápsulas de suco de laranja e colágeno hidrolisado, uma fonte de colágeno e de vitamina C, sendo assim, um produto com valor nutricional relevante.[006] The present invention refers in particular to microcapsules of orange juice and hydrolyzed collagen, a source of collagen and vitamin C, thus being a product with relevant nutritional value.

[007] A invenção BR 10 2019 001360 5 intitulada “Cápsula de polpa de maracujá com micro-organismo probiótico”, de 23 de janeiro de 2019, refere-se ao produto cápsula de polpa de maracujá com microrganismo probiótico obtido pelo processo de gelificação iônica da polpa de maracujá adicionada de microrganismos probióticos, o que difere do proposto neste documento que além de utilizar a laranja como fruta tem adição de colágeno.[007] The invention BR 10 2019 001360 5 entitled “Passion fruit pulp capsule with probiotic microorganism”, dated January 23, 2019, refers to the product passion fruit pulp capsule with probiotic microorganism obtained by the ionic gelation process of passion fruit pulp added with probiotic microorganisms, which differs from what is proposed in this document, which in addition to using orange as a fruit has the addition of collagen.

[008] A invenção BR 10 2018 00216 9 intitulada “Composição para preparo de bebida” de 31 de janeiro de 2018, refere-se a uma composição de pó para preparo de bebida à base de colágeno hidrolisado, que é uma combinação de colágeno do tipo i com colágeno do tipo ii e vitaminas c e d, o que difere do proposto neste documento que se trata de uma microcápsula de laranja com colágeno.[008] The invention BR 10 2018 00216 9 entitled “Composition for preparing drinks” of January 31, 2018, refers to a powder composition for preparing drinks based on hydrolyzed collagen, which is a combination of collagen from type i with type ii collagen and vitamins c and d, which differs from what is proposed in this document, which is an orange microcapsule with collagen.

[009] A invenção BRPI0804368 intitulada “Composição antioxidante compreendendo microcápsulas de camu-camu” de 13 de outubro de 2008, refere-se a composições antioxidantes compreendendo microcápsulas com um extrato vegetal, especialmente extratos de camu-camu, e um material encapsulante, o que difere do proposto neste documento que além do suco de laranja apresenta o colágeno como material a ser encapsulado.[009] The invention BRPI0804368 entitled “Antioxidant composition comprising camu-camu microcapsules” of October 13, 2008, refers to antioxidant compositions comprising microcapsules with a plant extract, especially camu-camu extracts, and an encapsulating material, the which differs from what is proposed in this document, which, in addition to orange juice, presents collagen as a material to be encapsulated.

[0010] O invento em questão difere das demais patentes e/ou pedidos de patente, disponíveis até o momento, devido ao seu caráter inovador para o desenvolvimento de microcápsulas de laranja com colágeno por gelificação iônica. Breve descrição dos desenhos A Figura 1 apresenta o processo de extração do suco de laranja. A Figura 2 apresenta o processo de obtenção das microcápsulas de laranja com colágeno.[0010] The invention in question differs from other patents and/or patent applications available to date, due to its innovative nature for the development of orange microcapsules with collagen by ionic gelation. Brief description of the drawings Figure 1 shows the orange juice extraction process. Figure 2 shows the process of obtaining orange microcapsules with collagen.

Descrição da invençãoDescription of the invention

[0011] Para a elaboração das microcápsulas de suco de laranja e colágeno hidrolisado realizou-se, primeiramente, a extração do suco de laranja com auxílio de um extrator de suco industrial.[0011] To prepare microcapsules of orange juice and hydrolyzed collagen, the orange juice was first extracted with the aid of an industrial juice extractor.

[0012] As laranjas foram cortadas ao meio e pressionadas no extrator industrial para remoção do suco e em seguido o suco foi coado para retirada dos caroços e bagaços da laranja.[0012] The oranges were cut in half and pressed in the industrial extractor to remove the juice and then the juice was strained to remove the orange seeds and pomace.

[0013] Para o processo de encapsulação foi utilizada a técnica de gotejamento, onde foram utilizados dois gelificantes diferentes: alginato de sódio e ágar ágar.[0013] For the encapsulation process, the dripping technique was used, where two different gelling agents were used: sodium alginate and agar agar.

[0014] Foram preparadas três soluções: Solução I (foram misturados 1,5g de alginato de sódio em solução de colágeno hidrolisado 5% (p/v) em suco de laranja), Solução II (foram misturados 3g de ágar ágar em solução de colágeno hidrolisado 5% (p/v) em suco de laranja), Solução III (solução de cloreto de cálcio 2% (p/v) em água filtrada).[0014] Three solutions were prepared: Solution I (1.5g of sodium alginate was mixed in a 5% (w/v) hydrolyzed collagen solution in orange juice), Solution II (3g of agar agar was mixed in a solution of hydrolyzed collagen 5% (w/v) in orange juice), Solution III (calcium chloride solution 2% (w/v) in filtered water).

[0015] Nessa técnica é utilizado um sistema simples e eficiente, onde as soluções I e II são colocadas, separadamente, em dispersores e desta forma são pressurizadas para um recipiente contendo 500mL de solução III e 500 mL de óleo de canola gelado, formando respectivamente, as microcápsulas esféricas com gelificantes alginato de sódio e ágar ágar.[0015] In this technique, a simple and efficient system is used, where solutions I and II are placed separately in dispersers and are thus pressurized into a container containing 500 mL of solution III and 500 mL of ice-cold canola oil, respectively forming , spherical microcapsules with gelling agents sodium alginate and agar agar.

[0016] As microcápsulas elaboradas foram acondicionadas em potes de polipropileno e armazenadas sob refrigeração a 5 ± 2°C.[0016] The microcapsules prepared were placed in polypropylene pots and stored under refrigeration at 5 ± 2°C.

Exemplos de concretizações da invençãoExamples of embodiments of the invention

[0017] A quantificação do teor de proteínas totais das microcápsulas de suco de laranja com o gelificante ágar foi significativamente maior que a das microcápsulas de suco de laranja com o gelificante alginato de sódio. Os teores das proteínas totais aumentaram consideravelmente ao final do armazenamento em ambas as formulações.[0017] The quantification of the total protein content of orange juice microcapsules with the agar gelling agent was significantly higher than that of the orange juice microcapsules with the sodium alginate gelling agent. Total protein levels increased considerably at the end of storage in both formulations.

[0018] Hidroxiprolina é considerado o principal componente do colágeno, sendo responsável por aproximadamente 13,5% dos aminoácidos (STOILOV et al., 2018). Os teores de hidroprolina nas duas formulações de microcápsulas apresentaram uma estabiliade até o final dos 16 dias de armazenamento.[0018] Hydroxyproline is considered the main component of collagen, accounting for approximately 13.5% of the amino acids (STOILOV et al., 2018). The hydroproline contents in the two microcapsule formulations showed stability until the end of 16 days of storage.

[0019] A adição de colágeno hidrolisado e suco de laranja na elaboração de microcápsulas potencializa as propriedades protéicas, bioativas e antioxidantes, tornando-as um produto inovador. O produto final possibilita a redução de uso de antioxidantes sintéticos e ainda permite uma melhor conservação, já que o pH do mesmo inibe o crescimento de alguns microorganismos.[0019] The addition of hydrolyzed collagen and orange juice in the preparation of microcapsules enhances the protein, bioactive and antioxidant properties, making them an innovative product. The final product makes it possible to reduce the use of synthetic antioxidants and also allows for better conservation, as its pH inhibits the growth of some microorganisms.

Claims (3)

1. MICROENCAPSULAÇÃO DE SUCO DE LARANJA (Citrus sinensis) E COLÁGENO HIDROLISADO POR GELIFICAÇÃO IÔNICA caracterizada pelo processo de gelificação iônica com a técnica de gotejamento, utilizando os gelificantes alginato de sódio e ágar ágar.1. MICROENCAPSULATION OF ORANGE JUICE (Citrus sinensis) AND HYDROLYZED COLLAGEN BY IONIC GELIFICATION characterized by the ionic gelation process with the dripping technique, using the gelling agents sodium alginate and agar agar. 2. MICROENCAPSULAÇÃO DE SUCO DE LARANJA (Citrus sinensis) E COLÁGENO HIDROLISADO POR GELIFICAÇÃO IÔNICA caracterizada pela preparação de três soluções: a) Solução I (foram misturados 1,5g de alginato de sódio em solução de colágeno hidrolisado 5% (p/v) em suco de laranja), b) Solução II (foram misturados 3g de ágar ágar em solução de colágeno hidrolisado 5% (p/v) em suco de laranja), c) Solução III (solução de cloreto de cálcio 2% (p/v) em água filtrada).2. MICROENCAPSULATION OF ORANGE JUICE (Citrus sinensis) AND HYDROLYZED COLLAGEN BY IONIC GELIFICATION characterized by the preparation of three solutions: a) Solution I (1.5g of sodium alginate was mixed in a 5% hydrolyzed collagen solution (w/v) in orange juice), b) Solution II (3g of agar agar was mixed in a 5% (w/v) hydrolyzed collagen solution in orange juice), c) Solution III (2% (w/v) calcium chloride solution v) in filtered water). 3. MICROENCAPSULAÇÃO DE SUCO DE LARANJA (Citrus sinensis) E COLÁGENO HIDROLISADO POR GELIFICAÇÃO IÔNICA de acordo com a reivindicação 2, caracterizada pelas soluções I e II serem colocadas, separadamente, em dispersores e pressurizadas para um recipiente contendo a solução III e óleo de canola, respectivamente, formando as microcápsulas esféricas.3. MICROENCAPSULATION OF ORANGE JUICE (Citrus sinensis) AND HYDROLYZED COLLAGEN BY IONIC GELIFICATION according to claim 2, characterized in that solutions I and II are placed separately in dispersers and pressurized into a container containing solution III and canola oil , respectively, forming spherical microcapsules.
BR102022018204-3A 2022-09-12 MICROENCAPSULATION OF ORANGE JUICE (CITRUS SINENSIS) AND HYDROLYZED COLLAGEN BY IONIC GELIFICATION BR102022018204A2 (en)

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