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BR0114575A - Sonda de hibridização de ácido nucléico de filamento duplo, reação de amplificação e detecção em tempo real, e, ensaio para detectar uma seq ência alvo de ácido nucléico - Google Patents

Sonda de hibridização de ácido nucléico de filamento duplo, reação de amplificação e detecção em tempo real, e, ensaio para detectar uma seq ência alvo de ácido nucléico

Info

Publication number
BR0114575A
BR0114575A BR0114575-4A BR0114575A BR0114575A BR 0114575 A BR0114575 A BR 0114575A BR 0114575 A BR0114575 A BR 0114575A BR 0114575 A BR0114575 A BR 0114575A
Authority
BR
Brazil
Prior art keywords
nucleic acid
real
detect
time detection
acid sequence
Prior art date
Application number
BR0114575-4A
Other languages
English (en)
Inventor
Qingge Li
Jixuan Liang
Guoyan Luan
Original Assignee
New York Health Res Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New York Health Res Inst filed Critical New York Health Res Inst
Publication of BR0114575A publication Critical patent/BR0114575A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

"SONDA DE HIBRIDIZAçãO DE áCIDO NUCLéICO DE FILAMENTO DUPLO, REAçãO DE AMPLIFICAçãO E DETECçãO EM TEMPO REAL, E, ENSAIO PARA DETECTAR UMA SEQœêNCIA ALVO DE áCIDO NUCLéICO". As sondas para detecção de ácido nucléico, que compreendem um par de oligonucleotídeos complementares rotulados de fluoróforos/ extintores, um dos quais é mais curto do que o outro, são capazes de detectar alvos de filamento único e de filamento duplo em reações de hibridização e reações de amplificações com detecção em tempo real. As sondas de filamento duplo de igual comprimento são úteis nas reações de amplificação de PCR com detecção em tempo real.
BR0114575-4A 2000-10-10 2001-10-05 Sonda de hibridização de ácido nucléico de filamento duplo, reação de amplificação e detecção em tempo real, e, ensaio para detectar uma seq ência alvo de ácido nucléico BR0114575A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN00130711 2000-10-10
CN01101446A CN1348096A (zh) 2000-10-10 2001-01-13 一种均相特异性检测核酸的探针及应用方法
PCT/US2001/031246 WO2002030946A1 (en) 2000-10-10 2001-10-05 Specific double-stranded probes for homogeneous detection of nucleic acid and their application methods

Publications (1)

Publication Number Publication Date
BR0114575A true BR0114575A (pt) 2004-01-06

Family

ID=25739622

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0114575-4A BR0114575A (pt) 2000-10-10 2001-10-05 Sonda de hibridização de ácido nucléico de filamento duplo, reação de amplificação e detecção em tempo real, e, ensaio para detectar uma seq ência alvo de ácido nucléico

Country Status (8)

Country Link
US (2) US7799522B2 (pt)
EP (1) EP1339732B1 (pt)
JP (1) JP3999653B2 (pt)
CN (2) CN1348096A (pt)
AU (2) AU2001296647B2 (pt)
BR (1) BR0114575A (pt)
CA (1) CA2424856C (pt)
WO (1) WO2002030946A1 (pt)

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Also Published As

Publication number Publication date
CA2424856C (en) 2014-03-11
US20110129828A1 (en) 2011-06-02
JP3999653B2 (ja) 2007-10-31
CN1348096A (zh) 2002-05-08
US20040023269A1 (en) 2004-02-05
CN1468251B (zh) 2012-11-21
EP1339732A4 (en) 2004-04-21
EP1339732B1 (en) 2012-08-01
WO2002030946A1 (en) 2002-04-18
CA2424856A1 (en) 2002-04-18
EP1339732A1 (en) 2003-09-03
AU9664701A (en) 2002-04-22
AU2001296647B2 (en) 2006-03-16
CN1468251A (zh) 2004-01-14
US7799522B2 (en) 2010-09-21
US9157112B2 (en) 2015-10-13
JP2004511227A (ja) 2004-04-15

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