BR0010398A - Microbiological production method alpha-l-aspartyl-l-phenylala-nina - Google Patents
Microbiological production method alpha-l-aspartyl-l-phenylala-ninaInfo
- Publication number
- BR0010398A BR0010398A BR0010398-5A BR0010398A BR0010398A BR 0010398 A BR0010398 A BR 0010398A BR 0010398 A BR0010398 A BR 0010398A BR 0010398 A BR0010398 A BR 0010398A
- Authority
- BR
- Brazil
- Prior art keywords
- asp
- phe
- new
- domain
- dna fragments
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 230000002906 microbiologic effect Effects 0.000 title abstract 2
- 108010069205 aspartyl-phenylalanine Proteins 0.000 abstract 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 3
- 239000012634 fragment Substances 0.000 abstract 3
- YZQCXOFQZKCETR-UWVGGRQHSA-N Asp-Phe Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 YZQCXOFQZKCETR-UWVGGRQHSA-N 0.000 abstract 2
- CKLJMWTZIZZHCS-UHFFFAOYSA-N Aspartic acid Chemical compound OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 abstract 2
- CKLJMWTZIZZHCS-UWTATZPHSA-N D-aspartic acid Chemical compound OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 abstract 2
- 230000005494 condensation Effects 0.000 abstract 2
- 238000009833 condensation Methods 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 108010016626 Dipeptides Proteins 0.000 abstract 1
- 230000006154 adenylylation Effects 0.000 abstract 1
- 229960005261 aspartic acid Drugs 0.000 abstract 1
- 210000004899 c-terminal region Anatomy 0.000 abstract 1
- 238000013048 microbiological method Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 229960005190 phenylalanine Drugs 0.000 abstract 1
- 210000003705 ribosome Anatomy 0.000 abstract 1
- 238000006177 thiolation reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"MéTODO DE PRODUçãO MICROBIOLóGICA PARA ALFA-L-ASPARTIL-L-FENILALA-NINA". A presente invenção refere-se a um novo método microbiológico para a produção de <244>-L-aspartil-L-fenilalanina (Asp-Phe) a partir dos substratos ácido L-aspártico (L-Asp) e L-fenilalanina (L-Phe) em que os substratos são contatados, na presença de ATP, com uma dipeptídeo sintetase não-ribossómica compreendendo dois módulos mínimos conectados por um domínio de condensação em que os módulos C-terminais N-resp. estão reconhecendo L-Asp e L-Phe, respectivamente, e o último módulo é covalentemente ligado em sua extremidade N-terminal para o domínio de condensação, e em que cada um destes módulos mínimos é composto de um domínio de adenilação e um co-fator 4'-fosfopanteteinila contendo domínio de tiolação, e que o Asp-Phe formado é recuperado. A presente invenção também se refere a novos fragmentos de DNA ou uma combinação de fragmentos de DNA codificando uma nova Asp-Phe dipeptídeo sintetase, microorganismos contendo estes fragmentos de DNA, assim como para a própria nova Asp-Phe dipeptídeo sintetase."MICROBIOLOGICAL PRODUCTION METHOD FOR ALFA-L-ASPARTIL-L-FENILALA-NINA". The present invention relates to a new microbiological method for the production of <244> -L-aspartyl-L-phenylalanine (Asp-Phe) from the substrates L-aspartic acid (L-Asp) and L-phenylalanine (L -Phe) in which the substrates are contacted, in the presence of ATP, with a non-ribosomal dipeptide synthase comprising two minimal modules connected by a condensation domain in which the N-resp C-terminal modules. are recognizing L-Asp and L-Phe, respectively, and the last module is covalently linked at its N-terminal end to the condensation domain, and where each of these minimal modules is composed of an adenylation domain and a co- factor 4'-phospopantheteinyl containing thiolation domain, and that the Asp-Phe formed is recovered. The present invention also relates to new DNA fragments or a combination of DNA fragments encoding a new Asp-Phe dipeptide synthase, microorganisms containing these DNA fragments, as well as to the new Asp-Phe dipeptide synthase itself.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99200954 | 1999-03-29 | ||
| EP99203518A EP1096011A1 (en) | 1999-05-10 | 1999-10-26 | Microbiological production method for alpha-l-aspartyl-l-phenylalanine |
| PCT/NL2000/000206 WO2000058478A1 (en) | 1999-03-29 | 2000-03-28 | MICROBIOLOGICAL PRODUCTION METHOD FOR α-L-ASPARTYL-L-PHENYLALANINE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0010398A true BR0010398A (en) | 2002-12-03 |
Family
ID=26153289
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0010398-5A BR0010398A (en) | 1999-03-29 | 2000-03-28 | Microbiological production method alpha-l-aspartyl-l-phenylala-nina |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1165799A1 (en) |
| JP (1) | JP2002539835A (en) |
| KR (1) | KR20010112931A (en) |
| CN (1) | CN1353757A (en) |
| AU (1) | AU3843900A (en) |
| BR (1) | BR0010398A (en) |
| CA (1) | CA2365594A1 (en) |
| EA (1) | EA200101015A1 (en) |
| MX (1) | MXPA01009875A (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4500978B2 (en) * | 2003-01-24 | 2010-07-14 | 味の素株式会社 | Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester |
| CN100362109C (en) * | 2003-01-24 | 2008-01-16 | 味之素株式会社 | Production method of α-L-aspartyl-L-phenylalanine-β-ester and production method of α-L-aspartyl-L-phenylalanine-α-methyl ester |
| JP2005040037A (en) * | 2003-07-25 | 2005-02-17 | Ajinomoto Co Inc | Method for producing dipeptide, peptide-producing enzyme using the same, and method for producing peptide-producing enzyme |
| US20050106703A1 (en) * | 2003-11-05 | 2005-05-19 | Kyowa Hakko Kogyo Co., Ltd. | Microorganisms producing dipeptides and process for producing dipeptides using the microorganisms |
| ATE449183T1 (en) * | 2004-06-25 | 2009-12-15 | Kyowa Hakko Bio Co Ltd | METHOD FOR PRODUCING DIPEPTIDES OR DIPEPTIDE DERIVATIVES. |
| US20080213827A1 (en) * | 2004-06-25 | 2008-09-04 | Hashimoto Shin-Ichi | Process For Producing Dipeptides or Dipeptide Derivatives |
-
2000
- 2000-03-28 EP EP00917475A patent/EP1165799A1/en not_active Withdrawn
- 2000-03-28 EA EA200101015A patent/EA200101015A1/en unknown
- 2000-03-28 KR KR1020017012410A patent/KR20010112931A/en not_active Withdrawn
- 2000-03-28 BR BR0010398-5A patent/BR0010398A/en not_active IP Right Cessation
- 2000-03-28 JP JP2000608758A patent/JP2002539835A/en active Pending
- 2000-03-28 CA CA002365594A patent/CA2365594A1/en not_active Abandoned
- 2000-03-28 AU AU38439/00A patent/AU3843900A/en not_active Abandoned
- 2000-03-28 CN CN00808250A patent/CN1353757A/en active Pending
- 2000-03-28 MX MXPA01009875A patent/MXPA01009875A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MXPA01009875A (en) | 2002-04-24 |
| EA200101015A1 (en) | 2002-02-28 |
| CA2365594A1 (en) | 2000-10-05 |
| AU3843900A (en) | 2000-10-16 |
| KR20010112931A (en) | 2001-12-22 |
| CN1353757A (en) | 2002-06-12 |
| EP1165799A1 (en) | 2002-01-02 |
| JP2002539835A (en) | 2002-11-26 |
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Legal Events
| Date | Code | Title | Description |
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| B08F | Application fees: dismissal - article 86 of industrial property law |
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| B08K | Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87) |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1927 DE 11/12/2007. |