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BE903937A - METHOD FOR THE IDENTIFICATION OF NUCLEIC ACIDS. - Google Patents

METHOD FOR THE IDENTIFICATION OF NUCLEIC ACIDS.

Info

Publication number
BE903937A
BE903937A BE0/216059A BE216059A BE903937A BE 903937 A BE903937 A BE 903937A BE 0/216059 A BE0/216059 A BE 0/216059A BE 216059 A BE216059 A BE 216059A BE 903937 A BE903937 A BE 903937A
Authority
BE
Belgium
Prior art keywords
identification
nucleic acids
affinity pair
detection probe
capture probe
Prior art date
Application number
BE0/216059A
Other languages
French (fr)
Inventor
H E Soderlund
Original Assignee
Orion Yhtymae Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orion Yhtymae Oy filed Critical Orion Yhtymae Oy
Publication of BE903937A publication Critical patent/BE903937A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)

Abstract

Méthode pour l'idenfication d'acides nucléiques, caractérisée en ce qu'on utilise au moins deux sondes qui sont dans la meme phase en solution dans une méthode d'hybridation, la sonde de détection étant marquée avec une substance de marquage détectable et un membre d'une paire d'affinité étant attaché à la sonde de capture, et qu'on isole l'hybride sonde de capture : acide nucléique cible : sonde de détection formé dans la réaction d'hybrication au moyen de l'autre membre de la paire d'affinité.Method for the identification of nucleic acids, characterized in that at least two probes are used which are in the same phase in solution in a hybridization method, the detection probe being labeled with a detectable labeling substance and a member of an affinity pair being attached to the capture probe, and the capture probe hybrid is isolated: target nucleic acid: detection probe formed in the hybridization reaction by means of the other member the affinity pair.

BE0/216059A 1985-01-02 1985-12-24 METHOD FOR THE IDENTIFICATION OF NUCLEIC ACIDS. BE903937A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI850004A FI72146C (en) 1985-01-02 1985-01-02 Procedure for Identifying Nucleic Acids.

Publications (1)

Publication Number Publication Date
BE903937A true BE903937A (en) 1986-04-16

Family

ID=8520136

Family Applications (1)

Application Number Title Priority Date Filing Date
BE0/216059A BE903937A (en) 1985-01-02 1985-12-24 METHOD FOR THE IDENTIFICATION OF NUCLEIC ACIDS.

Country Status (21)

Country Link
JP (1) JPH0669400B2 (en)
AT (1) AT397514B (en)
AU (1) AU561382B2 (en)
BE (1) BE903937A (en)
CA (1) CA1271705A (en)
CH (1) CH666696A5 (en)
DE (1) DE3546312A1 (en)
DK (1) DK164932C (en)
FI (1) FI72146C (en)
FR (1) FR2575493B1 (en)
GB (1) GB2169403B (en)
HU (1) HU196453B (en)
IE (1) IE58290B1 (en)
IL (1) IL77489A (en)
IT (1) IT1201514B (en)
LU (1) LU86238A1 (en)
NL (1) NL189427C (en)
NO (1) NO166743C (en)
RO (1) RO94651B (en)
SE (1) SE463212B (en)
ZA (1) ZA859895B (en)

Families Citing this family (37)

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US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US6060237A (en) * 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
IL79112A (en) * 1985-06-13 1992-01-15 Abbott Lab Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
FI76119C (en) * 1986-02-27 1988-09-09 Orion Yhtymae Oy QUANTITATIVE BEARING OF NUCLEIN SYRAMOLEKYLER AND REAGENTS FOERPACKNING SOM ANVAENDS VID FOERFARANDET.
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
FI894132A0 (en) * 1987-06-26 1989-09-01 Du Pont AFFINITETSAVLAEGSNING AV KONTAMINERANDE SEKVENSER UR KLONAD HYBRIDNUCLEINSYRA GENOM ATT ANVAENDA INFAONGANDE PAERLOR.
FI891524A7 (en) * 1987-07-31 1989-03-30 Gen Probe Inc Polynucleotide assay using oligonucleotides to prevent unwanted side reactions
EP0305145A3 (en) * 1987-08-24 1990-05-02 Ortho Diagnostic Systems Inc. Methods and probes for detecting nucleic acids
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
DE3800644A1 (en) * 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh PROCESS FOR HIGHLY SPECIFIC DETECTION OF NUCLEIC ACIDS IN SOLUTION
US5104791A (en) * 1988-02-09 1992-04-14 E. I. Du Pont De Nemours And Company Particle counting nucleic acid hybridization assays
ATE114726T1 (en) * 1988-05-10 1994-12-15 Du Pont METHODS FOR THE RAPID DETECTION OF NUCLEIC ACIDS.
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
US5082935A (en) * 1988-12-15 1992-01-21 Amoco Corporation Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
WO1990008841A1 (en) * 1989-02-06 1990-08-09 Gene-Trak Systems Probes and methods for the detection of listeria
WO1990010716A1 (en) 1989-03-10 1990-09-20 Gene-Trak Systems Immobilized oligonucleotide probes and uses therefor
AU5347690A (en) * 1989-03-10 1990-10-09 Gene-Trak Systems Preventing interference with affinity capture schemes
EP0484385A4 (en) * 1989-07-11 1993-05-26 Microprobe Corporation Quantification of bacteria using a nucleic acid hybridization assay
US5334501A (en) * 1989-07-11 1994-08-02 Microprobe Corporation Quantification of bacteria using a nucleic acid hybridization assay
GB8924989D0 (en) * 1989-11-06 1989-12-28 Scotgen Ltd Method and device for the detection of antibiotic resistance
US5580970A (en) * 1989-12-01 1996-12-03 Amoco Corporation Detection of HPV transcripts
AU7429591A (en) * 1990-04-18 1991-10-24 Gene-Trak Systems Nucleic acid probes for the detection of giardia lamblia
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
US7713528B1 (en) 1993-02-18 2010-05-11 Enzo Therapeutics, Inc. Method for in vivo delivery of active compounds using reagent conjugate
RU2146707C1 (en) * 1996-04-11 2000-03-20 Куликова Валентина Филипповна Method of detection of analyzed sequence of nucleic acid
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
GB0016833D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
US7465540B2 (en) 2000-09-21 2008-12-16 Luminex Corporation Multiple reporter read-out for bioassays
CN1303221C (en) * 2003-01-27 2007-03-07 英科新创(厦门)科技有限公司 Method for detecting target nucleic acid using affinity amplifying integrated signal group

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (en) * 1981-10-16 1983-07-11 Orion Yhtymae Oy MICROBIA DIAGNOSIS FOERFARANDE SOM GRUNDAR SIG PAO SKIKTSHYBRIDISERING AV NUCLEINSYROR OCH VID FOERFARANDET ANVAENDA KOMBINATIONER AV REAGENSER
FR2518755B1 (en) * 1981-12-23 1986-04-11 Pasteur Institut PROBE CONTAINING MODIFIED NUCLEIC ACID RECOGNIZABLE BY SPECIFIC ANTIBODIES AND USE OF THE SAME TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
EP0139489A3 (en) * 1983-09-26 1988-01-13 Ortho Diagnostic Systems Inc. Sandwich hybridization method for nucleic acid detection
ZA849596B (en) * 1983-12-12 1985-07-31 Miles Lab Hybridization assay with immobilization of hybrids by anti-hybrid binding
FR2558172B1 (en) * 1984-01-16 1986-06-13 Inst Nat Sante Rech Med PROBE CONTAINING MODIFIED NUCLEIC ACID AND RECOGNITION BY SPECIFIC ANTIBODIES AND USE OF SUCH PROBE AND THESE SPECIFIC ANTIBODIES TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE
CA1223222A (en) * 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
FR2560298B1 (en) * 1984-02-28 1988-07-15 Mors METHOD AND DEVICE FOR ADJUSTING THE PRECISION OF THE PRESSURE DROP OF A FLUID INITIALLY AT HIGH PRESSURE, SUCH AS A HYDRAULIC FLUID FOR EXAMPLE SUPPLYING A HYDRAULIC GROUP, AND APPLICATION OF THE METHOD TO THE DEVICE IN A HYDRAULIC GROUP OR PLANT INTEGRATED OR NOT, ESPECIALLY IN A MACHINE TABLE OR ANY TOOL SUPPORT DEVICE
NZ211453A (en) * 1984-03-22 1989-01-06 Biotechnology Research Enterpr Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor

Also Published As

Publication number Publication date
AT397514B (en) 1994-04-25
DE3546312A1 (en) 1986-07-10
IE58290B1 (en) 1993-08-25
IT8523408A0 (en) 1985-12-30
FR2575493B1 (en) 1990-01-26
FR2575493A1 (en) 1986-07-04
NO166743C (en) 1991-08-28
CA1271705A (en) 1990-07-17
SE8600011L (en) 1986-07-03
NO855308L (en) 1986-07-03
NO166743B (en) 1991-05-21
RO94651A (en) 1988-06-30
GB2169403A (en) 1986-07-09
DK164932B (en) 1992-09-07
ZA859895B (en) 1986-08-27
AU5174885A (en) 1986-07-17
HU196453B (en) 1988-11-28
GB2169403B (en) 1988-06-08
IT1201514B (en) 1989-02-02
GB8531414D0 (en) 1986-02-05
FI850004L (en) 1986-07-03
SE463212B (en) 1990-10-22
DK164932C (en) 1993-01-25
DK386D0 (en) 1986-01-02
DE3546312C2 (en) 1992-08-06
ATA376785A (en) 1993-09-15
SE8600011D0 (en) 1986-01-02
LU86238A1 (en) 1986-04-14
FI72146B (en) 1986-12-31
JPS61185200A (en) 1986-08-18
DK386A (en) 1986-07-03
RO94651B (en) 1988-07-01
CH666696A5 (en) 1988-08-15
NL189427C (en) 1993-04-01
FI72146C (en) 1987-04-13
HUT40166A (en) 1986-11-28
IL77489A (en) 1991-01-31
FI850004A0 (en) 1985-01-02
JPH0669400B2 (en) 1994-09-07
NL8503597A (en) 1986-08-01
IE853333L (en) 1986-07-02
NL189427B (en) 1992-11-02
AU561382B2 (en) 1987-05-07

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Legal Events

Date Code Title Description
RE20 Patent expired

Owner name: *SANGTEC MOLECULAR DIAGNOSTICS AB

Effective date: 20051224