[go: up one dir, main page]

AU6819500A - Method for characterizing nucleic acid fragments - Google Patents

Method for characterizing nucleic acid fragments

Info

Publication number
AU6819500A
AU6819500A AU68195/00A AU6819500A AU6819500A AU 6819500 A AU6819500 A AU 6819500A AU 68195/00 A AU68195/00 A AU 68195/00A AU 6819500 A AU6819500 A AU 6819500A AU 6819500 A AU6819500 A AU 6819500A
Authority
AU
Australia
Prior art keywords
oligomers
nucleic acid
array
acid fragments
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU68195/00A
Other versions
AU771491B2 (en
Inventor
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of AU6819500A publication Critical patent/AU6819500A/en
Application granted granted Critical
Publication of AU771491B2 publication Critical patent/AU771491B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention relates to a method for characterizing a nucleic acid fragment, in which the following method steps are carried out: a) immobilizing the nucleic acid fragment that is to be characterized on a surface; b) producing an array of oligomers on a second surface, whereby the oligomers are provided with a marker; c) removing the synthesized oligomers from the surface without vacating a predetermined region on the surface; d) bringing the surface, on which the nucleic acids to be characterized are immobilized, into contact with the oligomer array surface, whereby complementary oligomers of the array hybridize on the DNA to be characterized; e) removing non-complementary oligomers, and; f) detecting the complementary oligomers using markers thereof, whereby sequence information is determined using the position on the surface.
AU68195/00A 1999-07-28 2000-07-27 Method for characterizing nucleic acid fragments Ceased AU771491B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19935749 1999-07-28
DE19935749A DE19935749C2 (en) 1999-07-28 1999-07-28 Process for the characterization of nucleic acid fragments
PCT/DE2000/002595 WO2001009374A2 (en) 1999-07-28 2000-07-27 Method for characterizing nucleic acid fragments

Publications (2)

Publication Number Publication Date
AU6819500A true AU6819500A (en) 2001-02-19
AU771491B2 AU771491B2 (en) 2004-03-25

Family

ID=7916549

Family Applications (1)

Application Number Title Priority Date Filing Date
AU68195/00A Ceased AU771491B2 (en) 1999-07-28 2000-07-27 Method for characterizing nucleic acid fragments

Country Status (7)

Country Link
EP (1) EP1204763B1 (en)
JP (1) JP2003508024A (en)
AT (1) ATE278808T1 (en)
AU (1) AU771491B2 (en)
CA (1) CA2379163A1 (en)
DE (3) DE19935749C2 (en)
WO (1) WO2001009374A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002214811B2 (en) * 2000-11-13 2004-09-09 Human Genetic Signatures Pty Ltd Detection of methylated DNA molecules

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPR142500A0 (en) * 2000-11-13 2000-12-07 Human Genetic Signatures Pty Ltd A peptide nucleic acid-based assay for the detection of specific nucleic acid sequences
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
DE602004018801D1 (en) 2003-09-04 2009-02-12 Human Genetic Signatures Pty NUCLEIC DETECTION TEST
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
ATE476439T1 (en) 2004-09-10 2010-08-15 Human Genetic Signatures Pty AMPLIFICATION BLOCKERS COMPRISING INTERCALATING NUCLEIC ACIDS (INA) CONTAINING INTERCALATING PSEUDONUCLEOTIDES (IPN)
JP5116481B2 (en) 2004-12-03 2013-01-09 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド A method for simplifying microbial nucleic acids by chemical modification of cytosine
EP1883707B1 (en) 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
ATE531820T1 (en) 2005-09-14 2011-11-15 Human Genetic Signatures Pty HEALTH CONDITION TEST
CN101918595A (en) 2007-11-27 2010-12-15 人类遗传标记控股有限公司 Enzymes for amplification and replication of bisulfite-modified nucleic acids
CN103874766B (en) 2011-09-07 2017-07-18 人类遗传标记控股有限公司 Molecular detection assay

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
DE19625397A1 (en) * 1996-06-25 1998-01-08 Deutsches Krebsforsch Process for the combination of parallel oligonucleotide synthesis and preparation of oligomer chips
DE19754482A1 (en) * 1997-11-27 1999-07-01 Epigenomics Gmbh Process for making complex DNA methylation fingerprints
DE59812184D1 (en) * 1997-12-05 2004-12-02 Max Planck Gesellschaft METHOD FOR IDENTIFYING NUCLEIC ACIDS BY ELECTROSPRAY MASS SPECTROMETRY
US6908770B1 (en) * 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002214811B2 (en) * 2000-11-13 2004-09-09 Human Genetic Signatures Pty Ltd Detection of methylated DNA molecules

Also Published As

Publication number Publication date
ATE278808T1 (en) 2004-10-15
DE10082230D2 (en) 2002-01-03
EP1204763B1 (en) 2004-10-06
DE19935749A1 (en) 2001-02-08
DE19935749C2 (en) 2003-06-26
EP1204763A2 (en) 2002-05-15
DE50008148D1 (en) 2004-11-11
WO2001009374A2 (en) 2001-02-08
WO2001009374A3 (en) 2001-06-21
CA2379163A1 (en) 2001-02-08
AU771491B2 (en) 2004-03-25
JP2003508024A (en) 2003-03-04

Similar Documents

Publication Publication Date Title
AU8234998A (en) Categorising nucleic acid
AU3365095A (en) High throughput screening method for sequences or genetic alterations in nucleic acids
WO1999049082A3 (en) Modified nucleotides and methods useful for nucleic acid sequencing
WO2001048184A3 (en) Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
EP1961826A3 (en) A method for direct nucleic acid sequencing
WO2000056927A3 (en) Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
ATE551352T1 (en) PEPTIDE ACCEPTOR BINDING METHOD
WO2004011665A3 (en) Methods for fragmentation, labeling and immobilization of nucleic acids
AU6819500A (en) Method for characterizing nucleic acid fragments
WO2000024929A3 (en) Linear amplification mediated pcr (lam pcr)
DE60116651D1 (en) METHOD FOR THE AMPLIFICATION AND POSSIBLE CHARACTERIZATION OF NUCLEIC ACIDS
CA2244891A1 (en) Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
BR9407712A (en) Methods and compositions for effective nucleic acid sequencing
EP1816216A3 (en) Oligomer array with PNA and/or oligomers on a surface
WO2000070095A3 (en) Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
AU6704596A (en) Method for detecting nucleic acids using nucleotide probes permitting both specific capture and detection
ATE109204T1 (en) METHODS FOR IN VITRO DNA AMPLIFICATION, GENOMIC CLONING AND GENE MAPPING.
AU8162598A (en) Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
WO2005003318A3 (en) Assay and process for labeling and detection of micro rna and small interfering rna sequences
DE59608651D1 (en) METHOD FOR SEQUENCING BY OLIGOMER HYBRIDIZATION
WO2000022171A3 (en) Systems and methods for sequencing by hybridization
EP1041160A4 (en) METHOD FOR DETECTING MUTATION IN A BASE SEQUENCE
WO2004033485A3 (en) Nucleic acid supported protein complementation
AU781702C (en) Method and nucleic acids for determining the presence of micro-organisms specific to the brewing process
WO1997010366A3 (en) High throughput screening method for sequences or genetic alterations in nucleic acids

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase
TH Corrigenda

Free format text: IN VOL 15, NO 21, PAGE(S) 4494-4498 UNDER THE HEADING APPLICATIONS LAPSED, REFUSED OR WITHDRAWN PLEASE DELETE ALL REFERENCE TO APPLICATION NO. 68195/00 AND 69908/00

FGA Letters patent sealed or granted (standard patent)