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AU6678000A - Commercial production of chymosin in plants - Google Patents

Commercial production of chymosin in plants

Info

Publication number
AU6678000A
AU6678000A AU66780/00A AU6678000A AU6678000A AU 6678000 A AU6678000 A AU 6678000A AU 66780/00 A AU66780/00 A AU 66780/00A AU 6678000 A AU6678000 A AU 6678000A AU 6678000 A AU6678000 A AU 6678000A
Authority
AU
Australia
Prior art keywords
chymosin
commercial production
plants
seed
accumulate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU66780/00A
Inventor
Joseph Boothe
Richard Glenn Keon
Yin Shen
Gijs Van Rooijen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SemBioSys Genetics Inc
Original Assignee
SemBioSys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SemBioSys Genetics Inc filed Critical SemBioSys Genetics Inc
Publication of AU6678000A publication Critical patent/AU6678000A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0,5 % (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.
AU66780/00A 1999-08-23 2000-08-23 Commercial production of chymosin in plants Abandoned AU6678000A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37869699A 1999-08-23 1999-08-23
US09378696 1999-08-23
PCT/CA2000/000975 WO2001014571A1 (en) 1999-08-23 2000-08-23 Commercial production of chymosin in plants

Publications (1)

Publication Number Publication Date
AU6678000A true AU6678000A (en) 2001-03-19

Family

ID=23494182

Family Applications (1)

Application Number Title Priority Date Filing Date
AU66780/00A Abandoned AU6678000A (en) 1999-08-23 2000-08-23 Commercial production of chymosin in plants

Country Status (7)

Country Link
EP (1) EP1216306B1 (en)
AT (1) ATE400661T1 (en)
AU (1) AU6678000A (en)
CA (1) CA2381438C (en)
DE (1) DE60039442D1 (en)
MX (1) MXPA02001904A (en)
WO (1) WO2001014571A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087558A (en) 1998-07-22 2000-07-11 Prodigene, Inc. Commercial production of proteases in plants
AU2003232180A1 (en) * 2002-06-17 2003-12-31 Chr. Hansen A/S Improved method of producing an aspartic protease in a recombinant host organism
WO2005089040A2 (en) * 2004-03-23 2005-09-29 Chr. Hansen A/S Method of producing chymosin c and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD265164A1 (en) * 1987-09-09 1989-02-22 Akad Wissenschaften Ddr PROCESS FOR PREPARING PROTEINOGENIC ACTIVE SUBSTANCES
US5543576A (en) * 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use
DK162790D0 (en) * 1990-07-06 1990-07-06 Novo Nordisk As PLANT CELL
WO1992018634A1 (en) * 1991-04-09 1992-10-29 Unilever Plc Plant promoter involved in controlling lipid biosynthesis in seeds
KR100609886B1 (en) * 1997-04-25 2006-08-18 셈비오시스제네틱스인코포레이티드 Cleavage method of fusion protein

Also Published As

Publication number Publication date
ATE400661T1 (en) 2008-07-15
CA2381438C (en) 2010-10-05
CA2381438A1 (en) 2001-03-01
DE60039442D1 (en) 2008-08-21
EP1216306A1 (en) 2002-06-26
EP1216306B1 (en) 2008-07-09
WO2001014571A1 (en) 2001-03-01
MXPA02001904A (en) 2002-10-31

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase