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AU6114199A - Isolation method for nucleic acid and apparatus - Google Patents

Isolation method for nucleic acid and apparatus

Info

Publication number
AU6114199A
AU6114199A AU61141/99A AU6114199A AU6114199A AU 6114199 A AU6114199 A AU 6114199A AU 61141/99 A AU61141/99 A AU 61141/99A AU 6114199 A AU6114199 A AU 6114199A AU 6114199 A AU6114199 A AU 6114199A
Authority
AU
Australia
Prior art keywords
nucleic acid
filter
retentate
retained
cell lysate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU61141/99A
Inventor
Neil James Butt
Christopher Peter Jones
Andrew Martin Mitchell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Whatman BioScience Ltd
Original Assignee
Whatman BioScience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9822141.9A external-priority patent/GB9822141D0/en
Application filed by Whatman BioScience Ltd filed Critical Whatman BioScience Ltd
Publication of AU6114199A publication Critical patent/AU6114199A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for isolating nucleic acid which comprises: p1 (a) applying a sample comprising cells containing nucleic acid to a filter, whereby the cells are retained as a retentate and contaminants are removed; p1 (b) lysing the retentate from step (a) whilst the retentate is retained by the filter to form a cell lysate containing the nucleic acid; p1 (c) filtering the cell lysate with the filter to retain the nucleic acid and remove remaining cell lysate; p1 (d) optionally washing the nucleic acid retained by the filter; and p1 (e) eluting the nucleic acid, wherein the filter composition and dimensions are selected so that the filter is capable of retaining the cells and the nucleic acid.
AU61141/99A 1998-10-09 1999-10-08 Isolation method for nucleic acid and apparatus Abandoned AU6114199A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9822141.9A GB9822141D0 (en) 1998-10-09 1998-10-09 Isolation method and apparatus
GB9822141 1998-10-09
GB9918193A GB2346370B (en) 1998-10-09 1999-08-02 Nucleic acid isolation method and apparatus
GB9918193 1999-08-02
PCT/GB1999/003357 WO2000021973A1 (en) 1998-10-09 1999-10-08 Isolation method for nucleic acid and apparatus

Publications (1)

Publication Number Publication Date
AU6114199A true AU6114199A (en) 2000-05-01

Family

ID=26314493

Family Applications (1)

Application Number Title Priority Date Filing Date
AU61141/99A Abandoned AU6114199A (en) 1998-10-09 1999-10-08 Isolation method for nucleic acid and apparatus

Country Status (6)

Country Link
US (2) US20020010323A1 (en)
EP (1) EP1119576B1 (en)
AT (1) ATE242780T1 (en)
AU (1) AU6114199A (en)
DE (1) DE69908795T2 (en)
WO (1) WO2000021973A1 (en)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) * 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US6958392B2 (en) 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
AU2001263836A1 (en) * 2000-04-11 2001-10-23 Bureco Ag Method of nucleic acid recovery
GB2363381B (en) 2000-06-12 2004-11-24 Cambridge Molecular Tech Solution
AU2001286499A1 (en) * 2000-08-16 2002-02-25 Matthew Baker Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
WO2002042500A2 (en) * 2000-10-31 2002-05-30 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
CN1294262C (en) * 2000-10-31 2007-01-10 日立化学研究中心 Method for collecting and using nuclear mRNA
US20030003600A1 (en) * 2001-07-02 2003-01-02 Fuji Photo Film Co., Ltd. Biochemical analysis kit and method for exposing stimulable phosphor sheet
JPWO2003006650A1 (en) * 2001-07-09 2004-11-04 旭化成株式会社 Nucleic acid purification method and detection method using nonwoven fabric
GB0129662D0 (en) 2001-12-11 2002-01-30 Whatman Internat Ltd Stabilisation of double-stranded nucleic acids
FR2838066B1 (en) * 2002-04-09 2004-06-25 Genesystems METHOD AND AUTOMATE FOR EXTRACTING NUCLEIC ACIDS FROM A COMPLEX MIXTURE
WO2003091407A2 (en) * 2002-04-24 2003-11-06 Hitachi Chemical Research Center, Inc. Device and method for high-throughput quantification of mrna from whole blood
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
CA2501056C (en) * 2002-10-04 2012-12-11 Whatman, Inc. Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems
US20050227225A1 (en) * 2004-04-07 2005-10-13 Roche Molecular Systems, Inc. Stabilization of biomolecules in samples
DE102005047736B4 (en) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Method and system for isolating nucleic acids from any complex starting materials
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US20090111193A1 (en) 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
CN103173432B (en) 2011-12-22 2020-08-04 通用电气公司 Method and device for isolating nucleic acid
KR101915675B1 (en) 2012-02-07 2018-11-06 주식회사 미코바이오메드 ultra-fast device for the extraction of nucleic acids, and the method for the extraction of nucleic acids using the same
US9677981B2 (en) 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
US11214823B2 (en) 2015-12-22 2022-01-04 Canon U.S.A., Inc. Sample-to-answer system for microorganism detection featuring target enrichment, amplification and detection
CN105624152B (en) * 2016-03-01 2019-02-19 中国人民解放军第二军医大学 An instrument-free extraction method for yeast-like fungal total DNA for nucleic acid amplification
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
EP4124862A1 (en) 2017-05-05 2023-02-01 bioMérieux Method for detecting immune cellular response
KR102114148B1 (en) * 2018-01-03 2020-05-22 재단법인 구미전자정보기술원 Method for Detecting Foodborne Microorganism
JP7454264B2 (en) 2018-09-05 2024-03-22 ヒーロー サイエンティフィック リミテッド particle inspection
WO2021181338A1 (en) * 2020-03-11 2021-09-16 Hero Scientific Ltd. Testing for viruses and cellular biomarkers
EP4118412A1 (en) 2020-03-11 2023-01-18 Hero Scientific Ltd Testing devices
WO2022149135A2 (en) 2021-01-06 2022-07-14 Hero Scientific Ltd. Filtration sampling devices
KR102412078B1 (en) 2021-12-23 2022-06-22 홍승범 A method for nucleic acid extraction process using porous ion charge particles

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (en) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio METHOD FOR SEPARATING LONG CHAIN NUCLEIC ACIDS
DE4034036C2 (en) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Device and method for isolating nucleic acids from cell suspensions
US5652141A (en) * 1990-10-26 1997-07-29 Oiagen Gmbh Device and process for isolating nucleic acids from cell suspension
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
EP0814156B1 (en) * 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Method for the purification of DNA
JP2001501527A (en) * 1996-09-18 2001-02-06 プロメガ コーポレイション Improved filtration system and method

Also Published As

Publication number Publication date
WO2000021973A1 (en) 2000-04-20
EP1119576B1 (en) 2003-06-11
ATE242780T1 (en) 2003-06-15
US20020010323A1 (en) 2002-01-24
EP1119576A1 (en) 2001-08-01
DE69908795D1 (en) 2003-07-17
US20040054163A1 (en) 2004-03-18
DE69908795T2 (en) 2004-04-22

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase