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AU581819B2 - Process for the immortalization of human and animal cells - Google Patents

Process for the immortalization of human and animal cells

Info

Publication number
AU581819B2
AU581819B2 AU76771/87A AU7677187A AU581819B2 AU 581819 B2 AU581819 B2 AU 581819B2 AU 76771/87 A AU76771/87 A AU 76771/87A AU 7677187 A AU7677187 A AU 7677187A AU 581819 B2 AU581819 B2 AU 581819B2
Authority
AU
Australia
Prior art keywords
human
animal cells
immortalization
cell
animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU76771/87A
Other versions
AU7677187A (en
Inventor
Hinrich Johann Abken
Heinrich Barchet
Herbert Jungfer
Klaus Willecke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of AU7677187A publication Critical patent/AU7677187A/en
Application granted granted Critical
Publication of AU581819B2 publication Critical patent/AU581819B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

To immortalise human or animal cells by fusion with a non-replicable fragment of a cell which can be permanently cultured, and cultivation thereof in a culture medium without selection substances, the cell to be immortalised is used to transfect DNA obtained from the cytoplasm of a human or animal cell which can be permanently cultured.
AU76771/87A 1986-08-12 1987-08-11 Process for the immortalization of human and animal cells Ceased AU581819B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3627326 1986-08-12
DE19863627326 DE3627326A1 (en) 1986-08-12 1986-08-12 IMMORTALIZATION THROUGH DNS TRANSMISSION

Publications (2)

Publication Number Publication Date
AU7677187A AU7677187A (en) 1988-02-18
AU581819B2 true AU581819B2 (en) 1989-03-02

Family

ID=6307220

Family Applications (1)

Application Number Title Priority Date Filing Date
AU76771/87A Ceased AU581819B2 (en) 1986-08-12 1987-08-11 Process for the immortalization of human and animal cells

Country Status (7)

Country Link
EP (1) EP0256512B1 (en)
JP (1) JPH074237B2 (en)
AT (1) ATE87653T1 (en)
AU (1) AU581819B2 (en)
CA (1) CA1313832C (en)
DE (2) DE3627326A1 (en)
ZA (1) ZA875912B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ226750A (en) * 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
AU2921289A (en) * 1987-12-09 1989-07-19 Invitron Corporation Human cell life extension
DE4218945A1 (en) * 1992-06-10 1993-12-16 Boehringer Mannheim Gmbh DNA for immortalizing human or animal cells
EP0839183A1 (en) * 1995-06-23 1998-05-06 Micromet GmbH Immortalized epithelial tumor cell
JP2002201138A (en) * 2000-12-28 2002-07-16 Japan Tissue Engineering:Kk Angiogenesis inhibitor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3245665A1 (en) * 1982-05-04 1983-11-10 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR PRODUCING PERMANENT ANIMAL AND HUMAN CELL LINES AND THE USE THEREOF
US4652522A (en) * 1983-07-05 1987-03-24 The University Of Pennsylvania Continuous lymphocyte cell lines, their production and use
EP0207147A4 (en) * 1984-12-21 1988-07-14 Techniclone Res Partners Method for electrically immortalizing lymphoid cells.

Also Published As

Publication number Publication date
ZA875912B (en) 1988-02-12
EP0256512B1 (en) 1993-03-31
JPS6356283A (en) 1988-03-10
EP0256512A3 (en) 1989-12-06
DE3627326A1 (en) 1988-02-18
AU7677187A (en) 1988-02-18
CA1313832C (en) 1993-02-23
ATE87653T1 (en) 1993-04-15
EP0256512A2 (en) 1988-02-24
JPH074237B2 (en) 1995-01-25
DE3785109D1 (en) 1993-05-06

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Legal Events

Date Code Title Description
MK14 Patent ceased section 143(a) (annual fees not paid) or expired