AU3879300A - End selection in directed evolution - Google Patents
End selection in directed evolution Download PDFInfo
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- AU3879300A AU3879300A AU38793/00A AU3879300A AU3879300A AU 3879300 A AU3879300 A AU 3879300A AU 38793/00 A AU38793/00 A AU 38793/00A AU 3879300 A AU3879300 A AU 3879300A AU 3879300 A AU3879300 A AU 3879300A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/267,118 US6238884B1 (en) | 1995-12-07 | 1999-03-09 | End selection in directed evolution |
| US09267118 | 1999-03-09 | ||
| US09/276,860 US6352842B1 (en) | 1995-12-07 | 1999-03-26 | Exonucease-mediated gene assembly in directed evolution |
| US09276860 | 1999-03-26 | ||
| US09332835 | 1999-06-14 | ||
| US09/332,835 US6537776B1 (en) | 1999-06-14 | 1999-06-14 | Synthetic ligation reassembly in directed evolution |
| PCT/US2000/006497 WO2000053744A2 (fr) | 1999-03-09 | 2000-03-09 | Selection finale d'une evolution dirigee |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2005203719A Division AU2005203719A1 (en) | 1999-03-09 | 2005-08-18 | End selection in directed evolution |
Publications (1)
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| AU3879300A true AU3879300A (en) | 2000-09-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| AU38793/00A Abandoned AU3879300A (en) | 1999-03-09 | 2000-03-09 | End selection in directed evolution |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1161529A2 (fr) |
| JP (1) | JP2002537836A (fr) |
| AU (1) | AU3879300A (fr) |
| CA (1) | CA2361927A1 (fr) |
| IL (1) | IL145165A0 (fr) |
| WO (1) | WO2000053744A2 (fr) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6537776B1 (en) * | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US7390619B1 (en) | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| EP1119616A2 (fr) | 1998-10-07 | 2001-08-01 | Maxygen, Inc. | Rearrangement d'adn pour produire des acides nucleiques de detoxication contre les mycotoxines |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| IL138002A0 (en) | 1999-01-19 | 2001-10-31 | Maxygen Inc | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| IL138206A (en) * | 1999-02-04 | 2011-06-30 | Verenium Corp | Methods for non-stochastic generation of progeny polypeptides and hybrid polynucleotides |
| US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
| US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
| JP2003519495A (ja) | 2000-01-11 | 2003-06-24 | マキシジェン， インコーポレイテッド | 多様性生成およびスクリーニングのための一体化されたシステムおよび方法 |
| US7074590B2 (en) | 2000-06-23 | 2006-07-11 | Maxygen, Inc. | Chimeric promoters |
| EP1360290A2 (fr) | 2000-06-23 | 2003-11-12 | Maxygen, Inc. | Molecules costimulatrices |
| US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
| JP2002199890A (ja) * | 2000-10-23 | 2002-07-16 | Inst Of Physical & Chemical Res | 生分解性ポリエステル合成酵素の改変方法 |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| US6958217B2 (en) | 2001-01-24 | 2005-10-25 | Genomic Expression Aps | Single-stranded polynucleotide tags |
| CA2473308C (fr) * | 2001-01-24 | 2013-12-03 | Genomic Expression Aps | Dosage et kit d'analyse d'expression genique |
| ATE501251T1 (de) | 2001-01-25 | 2011-03-15 | Evolva Ltd | Zellbibliothek |
| US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
| WO2002092780A2 (fr) * | 2001-05-17 | 2002-11-21 | Diversa Corporation | Nouvelles molecules de liaison a un antigene destinees a des applications therapeutiques, diagnostiques, prophylactiques, enzymatiques, industrielles et agricoles et procedes de generation et de criblage de telles molecules |
| EP1504098B2 (fr) | 2002-05-17 | 2011-02-09 | Alligator Bioscience AB | Methode de developpement moleculaire in vitro d'une fonction de proteine |
| US20040219579A1 (en) * | 2003-02-19 | 2004-11-04 | Natasha Aziz | Methods of diagnosis of cancer, compositions and methods of screening for modulators of cancer |
| EP2502990B1 (fr) * | 2006-10-30 | 2015-07-22 | Promega Corporation | Protéines d'hydrolase mutante avec expressions cinétique et fonctionnelle améliorées |
| CN103476425B (zh) | 2007-03-30 | 2015-07-15 | 纽约州州立大学研究基金会 | 用于疫苗的减毒病毒 |
| WO2009088928A2 (fr) | 2007-12-31 | 2009-07-16 | Xoma Technology Ltd. | Librairies, réseaux et leurs utilisations pour une amélioration d'affinité ciblée |
| CN110498822A (zh) * | 2019-09-04 | 2019-11-26 | 上海药明康德新药开发有限公司 | DNA编码化合物库构建中on-DNA芳基叠氮化合物的合成方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5824469A (en) * | 1986-07-17 | 1998-10-20 | University Of Washington | Method for producing novel DNA sequences with biological activity |
| EP0285123A3 (fr) * | 1987-04-03 | 1989-02-01 | Stabra AG | Méthode de mutagénèse complète d'acides nucléiques |
| EP0527809B1 (fr) * | 1990-04-05 | 1995-08-16 | CREA, Roberto | Mutagenese "traversante" |
| US5512463A (en) * | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
| US5223408A (en) * | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
| US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5965408A (en) * | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| JP2001504325A (ja) * | 1996-09-27 | 2001-04-03 | マキシジェン，インコーポレイテッド | 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法 |
| GB9701425D0 (en) * | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| GB9712512D0 (en) * | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
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2000
- 2000-03-09 WO PCT/US2000/006497 patent/WO2000053744A2/fr not_active Ceased
- 2000-03-09 AU AU38793/00A patent/AU3879300A/en not_active Abandoned
- 2000-03-09 CA CA002361927A patent/CA2361927A1/fr not_active Abandoned
- 2000-03-09 IL IL14516500A patent/IL145165A0/xx unknown
- 2000-03-09 EP EP00917887A patent/EP1161529A2/fr not_active Withdrawn
- 2000-03-09 JP JP2000603365A patent/JP2002537836A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2361927A1 (fr) | 2000-09-14 |
| EP1161529A2 (fr) | 2001-12-12 |
| WO2000053744A2 (fr) | 2000-09-14 |
| WO2000053744A9 (fr) | 2001-06-28 |
| JP2002537836A (ja) | 2002-11-12 |
| WO2000053744A3 (fr) | 2001-01-18 |
| IL145165A0 (en) | 2002-06-30 |
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