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AU3252693A - Biochemical deactivation of RNA viruses - Google Patents

Biochemical deactivation of RNA viruses Download PDF

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Publication number
AU3252693A
AU3252693A AU32526/93A AU3252693A AU3252693A AU 3252693 A AU3252693 A AU 3252693A AU 32526/93 A AU32526/93 A AU 32526/93A AU 3252693 A AU3252693 A AU 3252693A AU 3252693 A AU3252693 A AU 3252693A
Authority
AU
Australia
Prior art keywords
rna
viruses
agents
deactivation
biochemical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU32526/93A
Inventor
Urs V. Wirth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
URS V WIRTH
Original Assignee
URS V WIRTH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by URS V WIRTH filed Critical URS V WIRTH
Publication of AU3252693A publication Critical patent/AU3252693A/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66Enzymes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Detergent Compositions (AREA)
  • Cosmetics (AREA)

Description

oUII-IIIIo..aI UdLLuVdLIUn u1 mXtim-viruses wiLn agents KaLu as anti-inrectives. 1 inventor and applicant: Dr. Urs V. Wirth; Sonnsyterain, CH- 6048 Horw, Switzerland. Priority date: 23rd June 1992 applied at BAGE in CH; PCT-application: 31st december 1992 at WIPO. Abstract: Ribonucleases (RNases) are highly active, easily used and extremely stable enzymes. Together with detergents, RNases deactivate and destroy irreversibly RNA-viruses. Agents containing RNase(s) and detergents are therefore suitable for disinfecting instruments, clothing and surfaces from contaminating RNA-viruses. Special application forms for coating the skin surfaces of human beings, animals or plants reduce the risk of infection from RNA-viruses. Agents with RNases and detergents, possibly combined with other substances, may be regarded as "anti infectives against RNA viruses (= aiRV)". DESCRIPTION: Ribonucleases (RNases) are extremly stable enzymes and highly active under a variety of assay conditions. RNases inactivate in conjunction with tensides (= detergents ) RNA-Viruses fast and irreversibly, thus this mixture can be designated as anti-infective against RNA-V iruses (aiRVi). AiRVi- mixtures containing RNases and detergents will be called below agents R&D. Agents R&D can be combined with different classical compounds (theoretically in all possible physical states and mixtures) and used for cleaning, disinfection and body care. In addition agents R&D might be combined with other substances and used as new optimized reagents with new formulations as well. Agents R&D are well suited to disinfect objects, such as tools or clothing, contaminated with RNA-viruses. Agents R&D applied to objects and especially to skin surfaces inclusive mucosa are suited to protect humans and/or animals against virulent RNA-viruses. Agents R&D can be used as well for plant protection against RNA-virus diseases. Agents R&D, if necessary combined with other compounds can be used for prevention of diseases caused by RNA-viruses. a) Examples and importance of RNA-viruses: RNA -viruses have as genetic material ribonucleic-acid ( RNA ) either alone or at least as important part: al) Among RNA-viruses are a large number of infectious agents of serious and frequently deadly diseases of mankind and animals: (Source: - : E. Wiesmann, Med. Mikrobiologie, Thieme-Verlag,1974; +: References on request) - Picornaviruses: -- Enteroviruses: -- Poliomyelitis Viruses: e.g. polio --- Coxsackieviren: e.g.. meningitis --- ECHO viruses: e.g. . meningitis & meningoencephalitis -- Rhinoviruses: e.g. common cold - Encephalomyocarditisviruses: - foot and mouth disease virus: - Reoviruses: e.g. Bluetongue, epizootic hemorrhagic disease & equine encephalitis - Arboviruses: e.g. encephalitis or encephalomyelitis - Myxoviruses: - Ortho-Myxoviruses: e.g.. Influenzaviruses -- Para-Myxoviruses.: e.g.. parainfluenza, mumps - Myxosimilar Viruses: e.g.. measles, rubella, canine distemper, rinderpest, hog cholera + certain Hepatitisviruses: e.g. inflammation and tumors of the liver + retroviruses: e.g. RNA-tumorviruses ++ RSV: e.g.. chicken sarcoma, leucosis, MMLV: murine leukemia virus, MMTV: murine mammary tumor virus ++ lentiviruses: e.g. : visna (animals); HTLV (human) P atent a: CH-01-957/92-3 -> PCT/CH92/00249 & WO 94/00016 (U.V.Wirth,1994-l) Biochemical deactivation of KNA-Viruses with agents K&U as anti-inTectives. 2 +++ HTLVIIl = HIV = LAV: (main-) cause for AIDS (acquired immune deficiency) of mankind and similar viruses of animals: e.g. : SIV (simian), BIV (bovine), FIV (feline) a2) RNA-Viruses causing diseases in plants: (source: F. Sch6nbeck, Pflanzenkrankheiten, Teubner, 1979) - leaf-Mosaic-virus: Como-, Tobamo-, Bromo-, and other groups. - Rhabdoviruses: - Reoviruses: b) Properties of RNases (e.g. RNase A): - Stability: heat stable (full activity even after boiling!) and stable under dry or wet conditions. - Activity: only slightly modified activity by pH (5-8 optimal) or by changes of salt concentrations (0.3 Mol/1 NaCI optimum) or by detergents (e.g. SLS= Sodium Lauryl Sulphate). c) Effects of RNase and detergents on RNA-Viruses: - RNA-Viruses are built from their genetic material RNA and a protecting shell (=capsid) made from proteins. Certain RNA-Viruses have in addition an outer membrane, consisting of lipids and proteins. -Agents R&D contain RNases and detergents, if necessary with additives for stabilization and/or enhancement of activity & stability and eventually combined with different classical compounds. As anti-infectiva against RNA-Viruses (aiRVi) such agents cause deactivation of RNA-viruses: Detergents (= tensides = amphiphilic substances) dissolve the membrane and break the capside, then RNases hydrolyze (destroy) the genetic material (RNA) of the viruses, these steps result in a complete destruction of the RNA-viruses. Detergents alone can reduce the infection potential of some RNA-viruses to a certain degree. Thus in addition to limit specific large scale uptake of viruses by host cells by detergents, every possibility of viral multiplication in host cells or their debris is completely eliminated. The novel additional destruction of the genetic material RNA by the agents R&D for complete inactivation might be relevant, because it has been recently shown, that e.g. polio virus multiplies its RNA in intact or even in lysed cells (Science News, Vol.140, p.390, 1991). d) Agents R&D: Agents R&D can be combined together with different, classical substances for cleaning, disinfection and body care or can be created as new optimized reagents in different combinations with new formulations: - For cleaning and disinfection one needs at least a RNase (e.g. RNaseA) and one detergent (e.g. Sodium Lauryl Sulfate). Basic research has shown, that RNases under extremely varying conditions effectively hydrolyze. - For protection against infections, agents R&D might be used in all body care products such as: body lotions, cremes, hair-shampoos, shower-gels, powders, mouth hygienic solutions, eye-care liquids, genital solutions, .... Depending on the products and their application, these agents act within a short time to deactivate RNA-Viruses (disinfectant) or deliver a long term deactivation potential against RNA-Viruses (e.g. durable protection film). For a desired long term protective effect, one needs agents which establish a resistent layer (protection film) on the surface (tools, clothes, skin, mucosa). Such agents, based on gels, oils or wax are in part already patented and are used in practice. e) Use of agents R&D: - In plant protection to disinfect contaminated tools and to prevent disease by a resistant protective layer (wax or oil) together with plant protection agents. - For animals to disinfect tools, living areas and animals themselves. - For humans to clean and disinfect contaminated tools, surfaces, clothes and even body surface (skin and mucosa). Especially high risk places such as living or resting rooms of RNA virus excreting persons; e.g. in hospitals, laboratories, consulting rooms, ... possibly schools, sport facilities, hotels. Furthermore these agents can be used for general prevention against RNA viruses. People with contact to infected persons or their contaminated belongings have a relative higher risk for infection (e.g. physicians, nurses, cleaning personnel,...) and could protect themselves with protective layers. Considering the AIDS causing HIV RNA-viruses, protection of high risk groups is extremely urgent. Agents with RNases could be added to classic mechanical and chemical contraceptiva. Condoms and femidoms as so far best indicated and most propagated way to protect against infection could be treated with agents containing agents R&D as coating film or agents R&D Patent: CH-01-957/92-3 -> PCT/CH92/00249 & WO 94/00016 (U.V.Wirth,1994-l)

Claims (4)

1. Agent for biochemical deactivation of RNA-viruses, consisting of ribonuclease(-s) = RNase(-s) together with at least one detergent.
2. Agent of claim 1 wherein it is used for cleaning, disinfection, body care or coating surfaces.
3. Agent of claim 1 wherein it is used for protection of mankind, animals and/or plants against infection by RNA-viruses. additional claim legalized by WIPO (4th October 1993):
4. Agent of claim 1 wherein it is used for targeted and local therapy of infectious diseases by RNA and DNA viruses or localised tumors of mankind and animals. Patent: CH-01-957/92-3 -> PCT/CH92/00249 & WO 94/00016 (U.V.Wirth,1994-l)
AU32526/93A 1992-06-23 1992-12-31 Biochemical deactivation of RNA viruses Abandoned AU3252693A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH1957/92A CH682541A5 (en) 1992-06-23 1992-06-23 Biochemical Inactivation of RNA viruses.
CH1957/92 1992-06-23
PCT/CH1992/000249 WO1994000016A1 (en) 1992-06-23 1992-12-31 Biochemical deactivation of rna viruses

Publications (1)

Publication Number Publication Date
AU3252693A true AU3252693A (en) 1994-01-24

Family

ID=4222554

Family Applications (1)

Application Number Title Priority Date Filing Date
AU32526/93A Abandoned AU3252693A (en) 1992-06-23 1992-12-31 Biochemical deactivation of RNA viruses

Country Status (4)

Country Link
AU (1) AU3252693A (en)
CA (1) CA2116661A1 (en)
CH (1) CH682541A5 (en)
WO (1) WO1994000016A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017046A (en) * 1998-01-21 2000-01-25 Markovic; Vladimir Wheelchair apparatus
KR100454594B1 (en) * 2002-07-22 2004-11-03 배흥규 Rice cutlet and process for manufacturing thereof
DE102004001590B4 (en) * 2004-01-09 2012-12-06 Gerhard Knapp Use of an active substance and a combination of active substances for inhibiting mite faeces and / or mold spores and / or plant pollen and spores and / or for denaturing the keratin of animal hair
MX394626B (en) 2014-07-31 2025-03-24 Kimberly Clark Co ALCOHOL-BASED NON-STICK COMPOSITION.
GB2547341B (en) 2014-07-31 2022-08-17 Kimberly Clark Co Anti-adherent composition
AU2015297023B2 (en) 2014-07-31 2019-11-07 Kimberly-Clark Worldwide, Inc. Anti-adherent composition
WO2016160006A1 (en) 2015-04-01 2016-10-06 Kimberly-Clark Worldwide, Inc. Fibrous substrate for capture of gram negative bacteria
MX2018008569A (en) 2016-01-28 2018-08-16 Kimberly Clark Co Anti-adherent composition against dna viruses and method of inhibiting the adherence of dna viruses to a surface.
AU2016408394B2 (en) 2016-05-26 2021-11-11 Kimberly-Clark Worldwide, Inc. Anti-adherent compositions and methods of inhibiting the adherence of microbes to a surface
WO2020070014A1 (en) * 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR6614M (en) * 1967-03-16 1969-01-13
US3849254A (en) * 1973-05-31 1974-11-19 Univ Virginia Process for effecting enzymatic reactions in aerosols
IL78703A (en) * 1985-05-10 1993-02-21 Benzon Alfred Method of producing extracellular enzymes, the enzymes produced thereby and compositions containing them; a hybrid plasmid and a dna fragment comprising dna encoding the enzymes; a microorganism harbouring the plasmid; and a method for removing nucleic acids from biological materials
HU204710B (en) * 1988-05-26 1992-02-28 Nika Health Products Inc Process for producing pharmaceutical compositions having antiviral and antibacterial effect
DE3939771A1 (en) * 1989-12-01 1991-06-06 Behringwerke Ag PROCESS FOR THE BIOLOGICAL INACTIVATION OF NUCLEIC ACIDS
DE4016483A1 (en) * 1990-05-22 1991-11-28 Klinger & Co Dr Treatment against phytopathogenic viruses - using nuclease(s) aq. soln. for plants, fungi and fungal spores
DE4024530A1 (en) * 1990-08-02 1992-02-06 Klinger & Co Dr Control of viral disease using bovine pancreatic nuclease(s) - for treatment of e.g. rabies, herpes, Epstein-Barr virus and cow-pox

Also Published As

Publication number Publication date
CA2116661A1 (en) 1994-01-06
WO1994000016A1 (en) 1994-01-06
CH682541A5 (en) 1993-10-15

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