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AU3107084A - Verfahren zur definierten verknupfung doppelhelikaler dna-fragmente mittels einer linker-dna sowie als linker-dna geeignete oligonucleotide - Google Patents

Verfahren zur definierten verknupfung doppelhelikaler dna-fragmente mittels einer linker-dna sowie als linker-dna geeignete oligonucleotide

Info

Publication number
AU3107084A
AU3107084A AU31070/84A AU3107084A AU3107084A AU 3107084 A AU3107084 A AU 3107084A AU 31070/84 A AU31070/84 A AU 31070/84A AU 3107084 A AU3107084 A AU 3107084A AU 3107084 A AU3107084 A AU 3107084A
Authority
AU
Australia
Prior art keywords
dna
linker
oligonucleotide
sequence
formula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU31070/84A
Inventor
Koster Hubert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU3107084A publication Critical patent/AU3107084A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Process for the definite cross-linking of DNA fragments by means of cohesive ends by replacing a first fragment by a linkage DNA, dividing the obtained DNA in the region of the linkage sequence during the formation of the cohesive end and cross-linking the obtained DNA having the cohesive end with a second DNA fragment, the cohesive ends of the first end of the second DNA fragment being complementary. As linkage DNA, an oligonucleotide is used which has the formula R?1 - (X)n? - R?2 wherein R?1 and R?2 represent, reading from left to right, identical tetranucleotide sequences, in themselves complementary, having the formula (II), or R?1 is a pentanucleotide sequence having the formula (III) and R?2 is a pentadesoxynucleotide sequence having the formula (IV), the central nucleotides being complementary sequences of R?1 and R?2 groups being identical, or else R?1 representing a tetranucleotide or pentanucleotide sequence which is not complementary in itself or an hexanucleotide sequence having the formula (V), (VI) or (VII) and R?2 a complementary nucleotide sequence enantiomorphous of R?1 and of the same length. X is a short intermediary compound having the form of an oligonucleotide comprising from 2 to 30 nucleotide units.
AU31070/84A 1983-07-22 1984-06-28 Verfahren zur definierten verknupfung doppelhelikaler dna-fragmente mittels einer linker-dna sowie als linker-dna geeignete oligonucleotide Abandoned AU3107084A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19833326520 DE3326520A1 (en) 1983-07-22 1983-07-22 METHOD FOR DEFINED LINKING OF DOUBLE-HELICAL DNA FRAGMENTS BY MEANS OF A LINKER DNA AND OLIGONUCLETIDES SUITABLE AS LINKER DNA
DE3326520 1983-07-22

Publications (1)

Publication Number Publication Date
AU3107084A true AU3107084A (en) 1985-03-04

Family

ID=6204698

Family Applications (1)

Application Number Title Priority Date Filing Date
AU31070/84A Abandoned AU3107084A (en) 1983-07-22 1984-06-28 Verfahren zur definierten verknupfung doppelhelikaler dna-fragmente mittels einer linker-dna sowie als linker-dna geeignete oligonucleotide

Country Status (7)

Country Link
EP (1) EP0149634B1 (en)
JP (1) JPH0665302B2 (en)
AT (1) ATE37900T1 (en)
AU (1) AU3107084A (en)
DE (2) DE3326520A1 (en)
IT (1) IT1198887B (en)
WO (1) WO1985000621A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01117789A (en) * 1987-10-29 1989-05-10 Ajinomoto Co Inc Compound for deleting dna, oligomer used therefor and its use
WO1993006122A1 (en) * 1991-09-27 1993-04-01 Allelix Biopharmaceuticals Inc. Duplex-forming, polynucleotide conjugates
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
ES2320431T3 (en) 2001-07-16 2009-05-22 Caprotec Bioanalytics Gmbh CAPTURE COMPOUNDS, COLLECTIONS OF THE SAME AND METHODS TO ANALYZE THE PROTEOM AND COMPLEX COMPOSITIONS.
DE602004030212D1 (en) 2003-01-16 2011-01-05 Caprotec Bioanalytics Gmbh METHOD FOR THE IDENTIFICATION OF NON-TARGETED PROTEINS ON MEDICINAL PRODUCTS
DE102016215127A1 (en) 2016-08-12 2018-02-15 Seleggt Gmbh Eggs examination device

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
EP0036258A3 (en) * 1980-03-14 1982-02-10 Cetus Corporation Process for producing aspartame
IL59690A (en) * 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
IE52122B1 (en) * 1980-08-25 1987-06-24 Univ California Somatostatin or somatostatin precursors

Also Published As

Publication number Publication date
DE3474566D1 (en) 1988-11-17
JPH0665302B2 (en) 1994-08-24
WO1985000621A1 (en) 1985-02-14
EP0149634B1 (en) 1988-10-12
JPS60501838A (en) 1985-10-31
IT8409460A0 (en) 1984-07-18
IT1198887B (en) 1988-12-21
DE3326520A1 (en) 1985-01-31
EP0149634A1 (en) 1985-07-31
ATE37900T1 (en) 1988-10-15

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