AU2255295A - Dietetic soy based product, method for production thereof and use thereof - Google Patents
Dietetic soy based product, method for production thereof and use thereofInfo
- Publication number
- AU2255295A AU2255295A AU22552/95A AU2255295A AU2255295A AU 2255295 A AU2255295 A AU 2255295A AU 22552/95 A AU22552/95 A AU 22552/95A AU 2255295 A AU2255295 A AU 2255295A AU 2255295 A AU2255295 A AU 2255295A
- Authority
- AU
- Australia
- Prior art keywords
- fact
- soy
- soy based
- dietetic
- aspergillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000005911 diet Nutrition 0.000 title claims description 30
- 230000000378 dietary effect Effects 0.000 title claims description 30
- 238000004519 manufacturing process Methods 0.000 title claims description 9
- 238000000034 method Methods 0.000 claims description 44
- 102000004190 Enzymes Human genes 0.000 claims description 34
- 108090000790 Enzymes Proteins 0.000 claims description 34
- 229940088598 enzyme Drugs 0.000 claims description 34
- 239000000047 product Substances 0.000 claims description 32
- 239000002994 raw material Substances 0.000 claims description 21
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 19
- 235000002949 phytic acid Nutrition 0.000 claims description 19
- 244000005700 microbiome Species 0.000 claims description 14
- 230000003381 solubilizing effect Effects 0.000 claims description 14
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 claims description 13
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims description 13
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 claims description 13
- 210000002421 cell wall Anatomy 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 claims description 13
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims description 13
- 241000228212 Aspergillus Species 0.000 claims description 12
- 235000013325 dietary fiber Nutrition 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 108010011619 6-Phytase Proteins 0.000 claims description 11
- 150000001720 carbohydrates Chemical class 0.000 claims description 11
- 229940085127 phytase Drugs 0.000 claims description 11
- 102000004169 proteins and genes Human genes 0.000 claims description 11
- 235000014633 carbohydrates Nutrition 0.000 claims description 10
- 235000013312 flour Nutrition 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 241000228215 Aspergillus aculeatus Species 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 229920001277 pectin Polymers 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 101001096557 Dickeya dadantii (strain 3937) Rhamnogalacturonate lyase Proteins 0.000 claims description 6
- 238000011282 treatment Methods 0.000 claims description 6
- 241000228245 Aspergillus niger Species 0.000 claims description 5
- 108010059820 Polygalacturonase Proteins 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 241000228143 Penicillium Species 0.000 claims description 2
- 241000223259 Trichoderma Species 0.000 claims description 2
- 235000013861 fat-free Nutrition 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 238000001728 nano-filtration Methods 0.000 claims description 2
- 108020004410 pectinesterase Proteins 0.000 claims description 2
- 239000012466 permeate Substances 0.000 claims description 2
- 108010035322 rhamnogalacturonan acetylesterase Proteins 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 description 64
- 108010030291 alpha-Galactosidase Proteins 0.000 description 10
- 102000005840 alpha-Galactosidase Human genes 0.000 description 9
- 239000012141 concentrate Substances 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000007858 starting material Substances 0.000 description 8
- 108010073771 Soybean Proteins Proteins 0.000 description 6
- 235000013322 soy milk Nutrition 0.000 description 6
- 229940001941 soy protein Drugs 0.000 description 6
- 229940071440 soy protein isolate Drugs 0.000 description 6
- 229920001542 oligosaccharide Polymers 0.000 description 5
- 150000002482 oligosaccharides Chemical class 0.000 description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 210000000936 intestine Anatomy 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 235000013527 bean curd Nutrition 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229930182830 galactose Natural products 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 238000005063 solubilization Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 1
- 235000003276 Apios tuberosa Nutrition 0.000 description 1
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000170226 Voandzeia subterranea Species 0.000 description 1
- 235000013030 Voandzeia subterranea Nutrition 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 235000007924 ground bean Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01011—Pectinesterase (3.1.1.11)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/30—Removing undesirable substances, e.g. bitter substances
- A23L11/33—Removing undesirable substances, e.g. bitter substances using enzymes; Enzymatic transformation of pulses or legumes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01015—Polygalacturonase (3.2.1.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01022—Alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/0104—Alpha-L-rhamnosidase (3.2.1.40)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01173—Rhamnogalacturonan galacturonohydrolase (3.2.1.173)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Beans For Foods Or Fodder (AREA)
- Soy Sauces And Products Related Thereto (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Description
DIETETIC SOY BASED PRODUCT, METHOD FOR PRODUCTION THEREOF
AND USE THEREOF
The invention comprises a dietetic soy based product comprising proteins and carbohydrates, a method for production thereof, and a use thereof. Dietetic soy based products comprising proteins and carbohydrates belong to the prior art. Examples are soy milk, soy isolate and soy concentrate. Such dietetic soy based products can be used as nutrients or as part of nutrients both intended for humans and animals. If used as a part of nutrients, e.g. fat and/or vitamins can be added in order to produce a full nutrient. The dietetic soy based product soy milk is a well known dietetic product for human nutrition, which can be produced from many different soy raw materials as starting materials, vide W. Shurtleff & A. Aoyagi, 1979, Tofu and soy milk production, The book of tofu, volume II. ISBN 0-933332-01-7. If full fat soy flour or defatted soy flour are used as starting materials the soy milk will contain high amounts of phytate, stachyose and raffinose, and the soy milk will contain almost no dietary fibres. A method for improving the taste and compositional quality of soy milk is utilization of soy protein concentrate as starting material in which the soluble oligosaccharide fraction including stachyose and raffinose has been eliminated. However, phytate will be present in relatively high amounts and the price of the product will be high due to the more expensive starting material and due to the cost price of the dietary fibre fraction that has to be added separately. Alternatively a soy protein isolate can be used as a starting material. However, also in this case phytate will not be substantially eliminated, and soy protein isolate is a high price raw material. In regard to the significance of phytate, stachyose, raffinose and dietary fibres the following can be noted.
Methods for eliminating phytate are described in the prior art. Absence of phytate is wanted as phytate binds the minerals in foods resulting in a decrease in mineral uptake. US Patent No. 5,248,804 describes the elimination of phytate by use of an ion exchange process. The soy protein free from phytate is claimed useful as a nutrient for humans. However, such a process will be relatively costly due to
loss in yield and costs for regeneration of ion exchange materials. WO 90/08476 describes the production of a low-phytate soy protein isolate or concentrate by use of a phytate degrading enzyme. This product, however, will contain relatively high amounts of oligosaccharides, which are not digestible in the human intestine. In US Patent No. 3,297,548 the use of phytase for addition to the feeds for animals has been described showing how the mineral uptake is improved by decreasing phytate in the feed.
The fraction of soluble carbohydrates in soy comprises oligo¬ saccharides which are not digestible in the human intestine, i.e. stachyose and raffinose. Fermentation of these oligosaccharides in the intestine leads to flatulence. One way of partly eliminating these oligosaccharides in a dietetic soy based product is the use of more expensive starting materials, such as soy protein concentrate. Also, they can be totally eliminated by use of soy protein isolate as the starting material. Recently an enzymatic method for reduction of these carbohydrates has been described, vide European Patent No. 0479596. α-galactosidase is used for hydrolysis of stachyose and raffinose.
The dietary fibre fraction (the plant cell wall polysaccharide fraction) exhibits a beneficial influence on the intestine. However, a problem exists in formulating a dietetic soy based produced with the dietary fibre fraction from soy as it has a high water binding capacity leading to high viscous formulations which limit the concentration of fibres.
Thus, the purpose of the invention is the provision of a dietetic soy based product of the above indicated kind, which contains only negligible amounts of phytate, stachyose and raffinose, which contains soluble dietary fibres in large amounts, which is relatively cheap, and which exhibits good organoleptic properties, a method for production thereof, and a use thereof.
The dietetic soy based product according to the invention comprising proteins and carbohydrates is characterized by the fact that the dietetic soy based product exhibits the following composition of fat free dry matter:
Proteins (N x 6.25) > 50%
Soluble dietary fibres > 5%
Phytate < 0.3%
Stachyose < 0.4%
Raffinose < 0.4%
Surprisingly it has been found that the dietetic soy based product according to the invention fulfils the purpose of the invention, as it contains negligible amounts of phytate, stachyose and raffinose, as it contains soluble dietary fibres in large amounts and as it surprisingly is relatively cheap, and exhibits good organoleptic properties.
Also the invention comprises a method for production of the dietetic soy product according to the invention, and the method is characterized by the fact that
a) a soy based raw material is suspended in water b) the following enzymes are added to the suspension:
1 ) phytase in an amount sufficient for reduction of the content of phytate to below 50% of the initial content, preferably below 10% of the initial content.
2) alfa-galactosidase in an amount sufficient for reduction of the content of stachyose and raffinose to below 50% of the initial content, preferably below 10% of the initial content.
3) plant cell wall polysaccharide solubilizing enzymes in an amount sufficient for solubilizing pectic substances in an amount above 10% of the insoluble carbohydrate fraction of the soy based raw material, and c) the thus formed, enzyme treated suspension is heat treated in order to inactivate the enzymes.
In this specification with claims phytase is to be understood as any enzyme, which is able to remove at least one phosphate group from phytate.
In this specification with claims α-galactosidase is to be understood as any enzyme which is able to produce galactose monomers from oligomers
containing α-linked galactose. The α-galactosidase activity determination method is described in the publication AF 204, which on request can be obtained from Novo Nordisk A/S, Novo Alle, DK-2880 Bagsvaerd, Denmark. The principle of AF 204 comprises that α-galactosidase (α-D-galactosidase-galactohydrolase, EC 3.2.1.22) hydrolyzesthe colourless p-nitrophenyl-α-D-galactopyranosidase (p-NPGAL) forming 4-nitrophenol, which is yellow in alkaline solution.
In this specification with claims pectic substances are to be understood as polymers comprising α-linked galacturonic acid in the main chain.
Even if some of the process steps (e.g. the treatment with phytase and the treatment with α-galactosidase) belongs to the prior art in another context, the combination of the above indicated steps comprising the specified enzyme treatments and the final heat treatment, is novel and generates a surprising effect, i.e. the formation of the dietetic soy based product according to the invention with the above indicated surprising characteristics. In regard to step a) it is to be understood that the following materials can be used as soy based raw materials: defatted soy flour, full fat soy flour, soy flakes, and soy concentrate. More generally it can be stated that the soy based raw material which is the starting material in the method according to the invention, can be defined as comprising the constituents of the soy bean which are provided without any further processing than dehulling and grinding the whole soy bean. A heat treatment of the dehulled and ground bean does not limit the use of the soy based raw material although a soy based raw material which has only been exposed to a limited heat treatment is preferred. The soy based raw material can also be defatted soy flakes or defatted soy flour as these soy based raw materials are available at very low cost. It could be an advantage also to use defatted soy flakes or defatted soy flour, as the absence of oil facilitates the method according to the invention. Removing the oil in an extraction process and refining it in a traditional way also results in a more bland tasting oil which can then be added to the final product when formulating the dietetic soy based product according to the invention. It is to be understood that also soy protein concentrate and soy protein isolate can be used as the soy based raw material in the process according to the invention. However, due to the price of this kind of raw materials the dietetic soy based
product according to the invention appearing as the end product will be more expensive and lack some of the wanted soy components. Soy protein concentrate contains only very low amounts of soluble sugars and also exhibits a low content of non-degradable carbohydrates. Thus, if soy protein concentrate is used as the soy based raw material in the method according to the invention dietary fibres will have to be added to the end product, and if soy protein isolate is used as the soy based raw material in the method according to the invention, soluble sugars and soluble components from the fibre fraction will have to be added to the end product.
In regard to step b) it is to be understood that the enzymes can be added in any order or simultaneously, and that pH and temperature of the suspension should be adjusted in such a manner that the enzymes exhibit a reasonable activity and stability. If the pH should be lowered, and an acid is used for pH adjustment, such acid should be chosen among food grade mineral acids and organic acids or combinations hereof, examples being hydrochloric acid, phosphoric acid, citric acid, lactic acid, glucone-delta-lactone, acetic acid, and malic acid, however, the pH can also be lowered by fermentation with pH lowering microorganisms. Furthermore, in regard to the plant cell wall solubilizing enzymes it is to be understood that these comprise enzymes, which can degrade the pectic substances of soy to soluble high molecular weight molecules, which appear as dietary fibres when analyzed by the method described in AOAC 15 (1990) 985.29, whereby the total fibre content is corrected for protein. The plant cell wall solubilizing enzymes should be added in an amount which is able to solubilize a significant part, i.e. above around 10%, of the pectic substances. A supplementary advantage in relation to the method according to the invention is the fact that it is possible to perform the entire enzyme addition step b) at the same pH, i.e. around 6.0, due to the fact that it is possible to select workable types of the three kinds of enzymes, which exhibit a good compromise in regard to activity and stability at a pH value around 6.0.
In regard to step c) it is to be understood that the heat treatment should not be carried out to such an extent that valuable parts of the formed dietetic soy based product are decomposed.
In regard to all the enzymes used in the method according to the invention it is to be understood that their origin is immaterial; thus, they may be produced by means of a microorganism, which exhibits a natural ability to produce the enzyme in question, or they may be produced by genetic engineering. A preferred embodiment of the method according to the invention is characterized by the fact that the soy based raw material is defatted soy flakes or defatted soy flour. In this case the oil from the bean can be utilized separately and the method according to the invention will become facilitated. Also an extraction of the oil and a subsequent processing of the oil in conventional processes will lead to an organoleptically better soy oil which can then be added to the final dietetic soy based product if wanted.
A preferred embodiment of the method according to the invention is characterized by the fact that the soy based raw material is a soy material with a nitrogen solubility index above 50%. The nitrogen solubility index (NSI) is defined in A.O.C.S. Method Ba 11-65, revised 1969. In this case the soy based raw material has been exposed to a low heat treatment only, and the final dietetic soy based product is fully soluble and is obtained in high yield.
A preferred embodiment of the method according to the invention is characterized by the fact that the phytase is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus niger. In this manner a phytase preparation with a high phytase activity can be used, and furthermore, an Aspergillus niger phytase preparation is available on the market.
A preferred embodiment of the method according to the invention is characterized by the fact that the alfa-galactosidase is producible by means of a microorganism belonging to the genera Aspergillus, Trichoderma, or Penicillium, preferably to the species Aspergillus niger, reference being made to WO 94/23022. In this manner an α-galactosidase preparation with a high α-galactosidase activity can be used, and furthermore corresponding α-galactosidase preparations are available on the market.
A preferred embodiment of the method according to the invention is characterized by the fact that the plant cell wall solubilizing enzymes comprise a
polygalacturonase, which is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus aculeatus, reference being made to WO 94/14952. In this manner a polygalacturonase with a high polygalacturonase activity can be used. A preferred embodiment of the method according to the invention is characterized by the fact that the plant cell wall solubilizing enzymes comprise a pectin methyl esterase, which is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus aculeatus, reference being made to WO 94/25575. When this enzyme is used together with polygalacturonase an increase in solubilization of pectic substances/fibres is obtained.
A preferred embodiment of the method according to the invention is characterized by the fact that the plant cell wall solubilizing enzymes comprise a rhamnogalacturonase, which is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus aculeatus, reference being made to WO 92/19728 and WO 94/20612. In this manner a rhamnogalacturonase with a high rhamnogalacturonase activity can be used.
A preferred embodiment of the method according to the invention is characterized by the fact that the plant cell wall solubilizing enzymes comprise a rhamnogalacturonan acetyl esterase, which is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus aculeatus, reference being made to WO 93/20190. When this enzyme is used together with rhamnogalacturonase an increase in solubilization of pectic substances/fibres is obtained. A preferred embodiment of the method according to the invention is characterized by the fact that the effluent from step b) is separated in a soluble and an insoluble fraction, that the insoluble fraction is discarded, and that the soluble fraction is further processed as indicated in step c). In this embodiment the final dietetic soy based product is soluble, thus making it easier to use the product as a constituent in a nutrient. Furthermore the organoleptic properties are improved, due to the fact that the insoluble fraction is eliminated.
A preferred embodiment of the method according to the invention is characterized by the fact that the pH of the effluent from step b) is adjusted to 7 - 9, preferably to 7.5 - 8.5, that the pH adjusted effluent is subsequently separated in a soluble and an insoluble fraction, that the insoluble fraction is discarded and that the soluble fraction is further processed as indicated in step c). In this embodiment the protein solubility of the final product is increased, and consequently the yield is increased.
A preferred embodiment of the method according to the invention is characterized by the fact that the heat treatment in step c) is carried out as a UHT treatment and a cooling in a flash process. By flash cooling off-flavors are removed, whereby a dietetic soy based product with better organoleptic properties is provided. The UHT treatment comprising a direct heating by mixing with steam under pressure secures an instant heating. Instant heating and instant cooling allows a more well-defined heat treatment and makes it easier to keep proteins and other components soluble. The holding time should be long enough to secure inactivation of the enzymes. Temperatures in the range of 120°C to 145°C and holding times from 3 to 30 seconds are preferred. However, it is to be understood that also indirectly heat treatment even at low temperatures down to 70°C and a corresponding longer holding time can be used, although the solubility and taste quality of the dietetic soy based product will decrease.
A preferred embodiment of the method according to the invention is characterized by the fact that the effluent from step c) is concentrated by nanofiltration by means of a salt permeable membrane, and that the permeate is discarded. The final dietetic soy product is the retentate. This embodiment allows production of the dietetic soy based product according to the invention as a concentrate, which simply and cheaply can be converted to a powder by spray drying, which powder in its turn can easily be stored and distributed. The concentration can also be performed by reverse osmosis or evaporation.
Also, the invention comprises a use of the dietetic soy based product according to the invention, as a nutrient or part of a nutrient, intended for humans and/or animals.
The invention will be illustrated by the following example.
EXAMPLE 1
62.8 g of defatted soy flour with high nitrogen solubility index (Cargil 200/90) was suspended in 337.2 g of water at 50°C resulting in a protein concentration of 8%. pH was adjusted to 6.0 by means of 6N HCI.
The mixture was divided into 2 lots:
1. Addition of enzymes as indicated below and reaction for 4 hours at
50°C.
α-galactosidase (Novo Nordisk SP415). Concentration 0.4% of the soy flour.
Phytase (Novo Nordisk PPQ 4415). Concentration 0.2% of the protein content.
Rhamnogalacturonase (Novo Nordisk SP568). Concentration 0.1% of the protein content.
2. Blank without enzyme addition. 4 hours at 50°C.
After 4 hours both lots were adjusted to pH 8.0 by means of 4N NaOH. After centrifugation, the supernatant was pasteurized and freeze dried.
Analysis of dried products:
Lot 1 Lot 2
Dry matter, % 95.2 98.2
Protein, % 59.2 60.2
Dietary fibre, % 5.7 2.5
Stachyose, % 0.36 5.30
Raffinose, % 0.20 1.40
Phytate, % 0.16 1.54
The content of stachyose and raffinose was determined by an HPLC method which included separation of the oligosaccharides on a Dionex CarboPac PA column and Pulsed Amperometric Detection as described by the Dionex Corporation (Sunnyval, CA) in: Analysis of Carbohydrates by Anion Exchange Chromatography with Pulsed Amperometric Detection (Dionex Technical Note TN20). The taste of the sample from lot 1 was described as more bland with less pronounced soy flavor and with a sweeter taste compared to the sample from lot 2.
Claims (15)
1. Dietetic soy based product comprising proteins and carbohydrates, characterized by the fact that the dietetic soy based product exhibits the following composition of fat free dry matter:
Proteins (N x 6.25) > 50%
Soluble dietary fibres > 5%
Phytate < 0.3%
Stachyose < 0.4%
Raffinose < 0.4%
2. Method for production of the dietetic soy product according to Claim
1 , characterized by the fact that
a) a soy based raw material is suspended in water b) the following enzymes are added to the suspension:
1 ) phytase in an amount sufficient for reduction of the content of phytate to below 50% of the initial content, preferably below 10% of the initial content.
2) alfa-galactosidase in an amount sufficient for reduction of the content of stachyose and raffinose to below 50% of the initial content, preferably below 10% of the initial content
3) plant cell wall polysaccharide solubilizing enzymes in an amount sufficient for solubilizing pectic substances in an amount above 10% of the insoluble carbohydrate fraction of the soy based raw material, and c) the thus formed, enzyme treated suspension is heat treated in order to inactivate the enzymes.
3. Method according to Claim 2, characterized by the fact that the soy based raw material is defatted soy flakes or defatted soy flour.
4. Method according to Claim 2, characterized by the fact that the soy based raw material is a soy material with a nitrogen solubility index above 50%.
5. Method according to Claims 2 - 4, characterized by the fact that the phytase is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus niger.
6. Method according to Claims 2 - 5, characterized by the fact that the alfa-galactosidase is producible by means of a microorganism belonging to the genera Aspergillus, Trichoderma, or Penicillium, preferably to the species Aspergillus niger.
7. Method according to Claims 2 - 6, characterized by the fact that the plant cell wall solubilizing enzymes comprise an polygalacturonase, which is producible by means of a microorganism belonging to the genes Aspergillus, preferably to the species Aspergillus aculeatus.
8. Method according to Claims 2 - 7, characterized by the fact that the plant cell wall solubilizing enzymes comprise a pectin methyl esterase, which is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus aculeatus.
9. Method according to Claims 2 - 8, characterized by the fact that the plant cell wall solubilizing enzymes comprise a rhamnogalacturonase, which is producible by means of a microorganism belonging to the genes Aspergillus, preferably to the species Aspergillus aculeatus.
10. Method according to Claims 2 - 9, characterized by the fact that the plant cell wall solubilizing enzymes comprise a rhamnogalacturonan acetyl esterase, which is producible by means of a microorganism belonging to the genus Aspergillus, preferably to the species Aspergillus aculeatus.
11. Method according to Claims 2 - 10, characterized by the fact that the effluent from step b) is separated in a soluble and an insoluble fraction, that the insoluble fraction is discarded, and that the soluble fraction is further processed as indicated in step c).
12. Method according to Claim 11 , characterized by the fact that the pH of the effluent from step b) before the separation is adjusted to 7 - 9, preferably 7.5 - 8.5.
13. Method according to Claims 2 - 12, characterized by the fact that the heat treatment in step c) is carried out as a UHT treatment and a cooling in a flash process.
14. Method according to Claims 2 - 13, characterized by the fact that the effluent from step c) is concentrated by nanofiltration by means of a salt permeable membrane, and that the permeate is discarded.
15. Use of the dietetic soy based product according to Claim 1 as a nutrient or part of a nutrient, intended for humans and/or animals.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK393/94 | 1994-04-06 | ||
| DK39394 | 1994-04-06 | ||
| PCT/DK1995/000148 WO1995027406A1 (en) | 1994-04-06 | 1995-04-06 | Dietetic soy based product, method for production thereof and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2255295A true AU2255295A (en) | 1995-10-30 |
| AU681280B2 AU681280B2 (en) | 1997-08-21 |
Family
ID=8093005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU22552/95A Ceased AU681280B2 (en) | 1994-04-06 | 1995-04-06 | Dietetic soy based product, method for production thereof and use thereof |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0753999A1 (en) |
| JP (1) | JPH09512164A (en) |
| AU (1) | AU681280B2 (en) |
| WO (1) | WO1995027406A1 (en) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997037547A1 (en) * | 1996-04-09 | 1997-10-16 | E.I. Du Pont De Nemours And Company | Novel isoflavone-enriched soy protein product and method for its manufacture |
| GB2340727B (en) * | 1998-08-19 | 2002-05-22 | Univ Saskatchewan | Process for converting phytate into inorganic phosphate |
| US6284502B1 (en) | 1998-08-21 | 2001-09-04 | University Of Saskatchewan | Process for converting phytate into inorganic phosphate |
| JP2000300185A (en) * | 1999-04-16 | 2000-10-31 | Fuji Oil Co Ltd | Method for producing soy protein |
| CA2343545A1 (en) * | 2000-04-10 | 2001-10-10 | Kraft Foods, Inc. | Low-cost soy proteinaceous food ingredients |
| ATE321459T1 (en) | 2000-08-18 | 2006-04-15 | Solae Holdings Llc | SOY PROTEIN PRODUCT AND METHOD FOR PRODUCTION |
| AU2002215143A1 (en) * | 2000-11-15 | 2002-05-27 | Chemical Consultants (Pty) Ltd | A method for producing protein powder from legumes |
| CA2363451C (en) | 2001-11-20 | 2005-05-10 | Mcn Bioproducts Inc. | Oilseed processing |
| IL163933A0 (en) * | 2002-03-13 | 2005-12-18 | Solae Llc | Soy protein concentrate with low non-digestible oligosaccharides and process for its production |
| US20070014910A1 (en) * | 2005-07-18 | 2007-01-18 | Altemueller Andreas G | Acidic, protein-containing drinks with improved sensory and functional characteristics |
| AU2009307003B2 (en) | 2008-10-21 | 2014-12-11 | Burcon Nutrascience (Mb) Corp. | Production of soluble protein solutions from soy ("S701") |
| US8563071B2 (en) | 2008-10-21 | 2013-10-22 | Burcon Nutrascience (Mb) Corp. | Production of soluble protein solutions from soy (“S701” CIP) |
| US20110318454A1 (en) * | 2009-03-20 | 2011-12-29 | Novozymes A/S | Nutritional beverage and a method of making the same |
| US9155323B2 (en) * | 2009-05-15 | 2015-10-13 | Siebte Pmi Verwaltungs Gmbh | Aqueous process for preparing protein isolate and hydrolyzed protein from an oilseed |
| KR20190077104A (en) * | 2011-12-02 | 2019-07-02 | 프레리 아쿠아 테크 | Microbial-based process for high-quality protein concentrate |
| JP5682697B1 (en) * | 2013-12-24 | 2015-03-11 | 不二製油株式会社 | Plant-derived isolated protein and method for producing the same |
| EP3193627B2 (en) | 2014-09-18 | 2023-02-22 | DSM IP Assets B.V. | Method for producing an oil seed protein mix |
| CN111357826B (en) * | 2018-12-25 | 2022-11-22 | 丰益(上海)生物技术研发中心有限公司 | Special powder for traditional taste bean products and products thereof |
| FR3127370B1 (en) * | 2021-09-24 | 2025-04-18 | Roquette Freres | METHOD FOR REDUCING THE BITTERNESS OF A LEGUMINE PROTEIN |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE380343T1 (en) * | 1989-01-25 | 1991-03-21 | Alko Ltd., Helsinki | METHOD FOR PRODUCING PHYTATE-FREE OR LOW-PHYTATE ISOLATES AND CONCENTRATES OF SOY PROTEINS. |
| US5100679A (en) * | 1990-10-03 | 1992-03-31 | Cargill B.V. | Method of making a modified proteinaceous product and composition thereof |
-
1995
- 1995-04-06 AU AU22552/95A patent/AU681280B2/en not_active Ceased
- 1995-04-06 JP JP7526024A patent/JPH09512164A/en active Pending
- 1995-04-06 WO PCT/DK1995/000148 patent/WO1995027406A1/en not_active Ceased
- 1995-04-06 EP EP95915820A patent/EP0753999A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO1995027406A1 (en) | 1995-10-19 |
| JPH09512164A (en) | 1997-12-09 |
| EP0753999A1 (en) | 1997-01-22 |
| AU681280B2 (en) | 1997-08-21 |
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