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AU2019202785A1 - Granular Fertilizer and Manufacturing Method Thereof - Google Patents

Granular Fertilizer and Manufacturing Method Thereof Download PDF

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Publication number
AU2019202785A1
AU2019202785A1 AU2019202785A AU2019202785A AU2019202785A1 AU 2019202785 A1 AU2019202785 A1 AU 2019202785A1 AU 2019202785 A AU2019202785 A AU 2019202785A AU 2019202785 A AU2019202785 A AU 2019202785A AU 2019202785 A1 AU2019202785 A1 AU 2019202785A1
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Australia
Prior art keywords
granule
granular fertilizer
oil
group
bacillus
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AU2019202785A
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AU2019202785B2 (en
Inventor
Han-Yu Hsu
Wan-Jung Kao
Shean-Yun Khoo
Chien-Yi Liu
Alan Darmasaputra Ong
Kanha Panutta
Yu-Chien Su
Wei-sheng WEI
Yi-Jhih Yang
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ADVANCED GREEN BIOTECHNOLOGY Inc
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ADVANCED GREEN BIOTECHNOLOGY Inc
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  • Glanulating (AREA)

Abstract

A granular fertilizer and a method of manufacturing the granular fertilizer are provided. The method comprises a step of molding or rolling a material into a granule, a step of preparing a solution by mixing at least one surface additive with an oil, a step of spraying the solution on the granule to form a layer on the surface of the granule, and a step of drying the granule to form the granular fertilizer. MOLDING OR ROLLING A MATERIAL TO SOl FORM A GRANULE \/ PREPARING A SOLUTION BY DISPERSING AT LEAST ONE SURFACE ADDITIVE IN AN S02 \/ SPRAYING THE SOLUTION ON THE S03 GRANULE TO FORM A LAYER ON THE SURFACE OF THE GRANULE \/ DRYING THE GRANULE TO FORM THE S04 GRANULAR FERTILIZER

Description

GRANULAR FERTILIZER AND METHOD OF MANUFACTURING THEREOF
CROSS-REFERENCE TO RELATED APPLICATION AND CLAIM OF PRIORITY [0001] This application claims the benefit of Taiwanese Patent Application No.
107113428, filed on April 20, 2018, in the Taiwan Intellectual Property Office, the disclosure of which is incorporated herein in its entirety by reference.
1. Technical Field [0002] Some embodiments of the present invention provide a granular fertilizer and a method of manufacturing the granular fertilizer. The method is characterized by a step of spraying a mixture of a surface additive and oil on the surface of a granule to obtain the granular fertilizer.
2. Description of the Related Art [0003] Bio-fertilizers, especially microbial fertilizers, earn huge attention recently.
They are supplied to vitalize lands, promote plant growth, enhance plant resistance, and elevate the quality and quantity of crops [0004] Bio-fertilizers are provided in either liquid form or solid (powder) form.
The production costs for liquid bio-fertilizers are lower. However, liquid bio-fertilizers rapidly deteriorate and therefore offer shorter shelf-life; they are also easy to leak and cause inconvenience during transportation. On the other hand, solid bio-fertilizers offer higher stability and longer shelf-life; they are easy to transport as well. But when it comes to administration, powders have their disadvantages. Powders are easily blown away by airflows and are harmful to people as airborne particles. The administration of powder is also largely limited by the terrain.
[0005] Bio-fertilizers are frequently mixed with inorganic salts. Because of the high hygroscopicity of inorganic salts, such bio-fertilizers forms clots easily after
2019202785 18 Apr 2019 absorbing water from the air. Clots may diminish the appearance and the quality of bio-fertilizers, not to mention they are difficult to transport and administer.
SUMMARY [0006] Liquid bio-fertilizers are infamous for their short shelf-life, tendency to leak, and inconvenience during transportation. Solid bio-fertilizers are easily blown away by airflows and have high hygroscopicity. The high hygroscopicity usually leads to clotting and cause inconvenience during transportation and administration.
[0007] At least one embodiment of the present invention is proposed to resolve the disadvantages above. In one embodiment, a granular fertilizer is provided. The granular fertilizer comprises a granule and a layer on the surface of the granule. More particularly, the layer comprises an oil and at least one surface additive dispersed in the oil. Moreover, the granular fertilizer may also comprise an additive based on demands.
[0008] In another embodiment, a method of manufacturing a granular fertilizer is provided. The method comprises a step of molding or rolling a material to form a granule, a step of preparing a solution by dispersing at least one surface additive in an oil, a step of spraying the solution on the granule to form a layer on the surface of the granule, and a step of drying the granule to form the granular fertilizer.
[0009] There are many embodiments described and illustrated herein. The present inventions are neither limited to any single aspect nor embodiment thereof, nor to any combinations and/or permutations of such aspects and/or embodiments. Moreover, each of the aspects of the present inventions, and/or embodiments
2019202785 18 Apr 2019 thereof, may be employed alone or in combination with one or more of the other aspects of the present inventions and/or embodiments thereof. For the sake of brevity, many of those permutations and combinations will not be discussed separately herein.
BRIEF DESCRIPTION OF THE DRAWINGS [0010] FIG. 1 is a flowchart illustrating a method of manufacturing a granular fertilizer, in accordance with one embodiment of the present invention.
[0011] FIG. 2 is a flowchart illustrating a method of manufacturing a granular fertilizer, in accordance with another embodiment of the present invention.
DESCRIPTION OF THE PREFERRED EMBODIMENTS [0012] The drawings disclose some preferred embodiments of the present invention, which are intended to be used with the descriptions herein to enable one skilled in the art to understand the claimed features, as well as to make and use the claimed invention.
[0013] FIG. 1 is a flowchart illustrating a method of manufacturing a granular fertilizer, in accordance with one embodiment of the present invention. The method begins with a step (S01) of molding or rolling a material to form a granule. The material may be urea, calcium carbonate (CaCCh), calcium sulfate (CaSOff, magnesium sulfate (MgSOff, peat soil, kaolinite, clay, bentonite, diatomaceous earth, acid clay, or the combination thereof. The material may be known compound fertilizers or organic fertilizers, such as No.43 Compound Fertilizer, No.39 Compound Fertilizer, or No.5 Compound Fertilizer obtained from Taiwan Fertilizer Co., Ltd.
2019202785 18 Apr 2019 [0014] In this embodiment, the particle size of the granule which may be formed by molding or rolling in the step (S01) is not over 325 mm.
[0015] The next step (S02) of the method is to prepare a solution by dispersing at least one surface additive in an oil. The at least one surface additive is homogeneously mixed with the oil to form the solution. The oil is one selected from the group consisting of mineral oil, base oil, lubricating oil, vegetable oil (e.g., palm oil, soybean oil), and the combination thereof. The oil is characterized by the huge quantity of unsaturated hydrocarbons in it.
[0016] In this step (S02), the at least one surface additive may be a bacterial powder. The bacterial powder comprises phosphate-solubilizing microorganisms (PSM), actinobacteria, saccharomyces, Trichoderma, fungi, or the combination thereof. In some preferred embodiment, the bacterial powder comprises Bacillus licheniformis, Bacillus amyloliquefaciens, Pseudomonas fluorescens, Bacillus mycoides AGB01, Bacillus subtilis, or the combination thereof. The at least one surface additive may be calcium lignosulfonate, sodium lignosulfonate, biochar, humic acid, amino acid, micronutrient, and the combination thereof. The granule and the at least one micronutrient together may serve as sources of nutrients. The as least one micronutrient may be B, Ca, Cl, Co, Cu, Fe, Mn, Mo, Ni, Na, Zn, or the combination thereof, and provided as salts, like sulfates, nitrates, and halides.
[0017] The particle size of the at least one surface additive dispersed in the oil is not over 60 mesh.
[0018] The 16S rDNAof Bacillus amyloliquefaciens comprises a nucleic acid sequence of SEQ ID No. 1, the 16S rDNA of Bacillus mycoides AGB01 comprises a nucleic acid sequence of SEQ ID No. 2, and the 16S rDNA of Bacillus subtilis comprises a nucleic acid sequence of SEQ ID No. 3.
[0019] Following the second step (S02) is a step (S03) of spraying the solution on
2019202785 18 Apr 2019 the granule to form a layer on the surface of the granule. The solution comprising the surface additive and oil is atomized by a device and then sprayed on the surface of granule to form a layer. The layer fixes the surface addictive (e.g., a bacterial powder, sodium lignosulfonate, biochar, humic acid, amino acid) on the surface of the granule to elevate the nutrient content of the granule. It also protects the fertilizer from clotting, as well as maintains the appearance and quality of the fertilizer, by isolating the granule from humidity with the oil.
[0020] During the step of spraying, it is suggested to keep the granule and the device at an appropriate distance. If the distance is too short, the solution may bounce off the granule and decrease the coverage and integrity of the layer; if the distance is too far, the adhesiveness of the solution significantly decreases as the temperature falling, and thus failing to be attached on the surface of the granule.
[0021] In the fourth step (S04), granule is dried to form the granular fertilizer. The granule with the layer obtained in the third step (S03) is send to a machine for air-drying with cold or hot air to prevent clotting. Once pass the inspection, the manufacturing of granular fertilizer is completed.
[0022] FIG. 2 is a flowchart illustrating a method of manufacturing a granular fertilizer, in accordance with another embodiment of the present invention. The method comprises a step (Sil) of molding or rolling a mixture of an additive and a material to form a granule, a step (S12) of preparing a solution by dispersing at least one surface additive in an oil, a step (SI 3) of spraying the solution on the granule to form a layer on the surface of the granule, and a step of drying the granule to form the granular fertilizer.
[0023] The embodiments in FIG. 1 and FIG. 2 are slightly different at the step of molding or rolling. In FIG. 2, the additive is added to the material, and then molding or rolling them into granule. The additive here may be an adhesive,
2019202785 18 Apr 2019 anticaking agent, biocide, antioxidant, reducing agent, surfactant, or the combination thereof. The selection criteria of additive may be based on the characteristics of the material used.
[0024] The adhesive may be xanthan gum, gelatin, arabic gum, clay, or the combination thereof. Some other examples of adhesives include poly(ethylene-vinyl acetate) (PEVA), chloroprene rubber phenolic adhesive, poly(oxyethylene) (PEG), and methyl methacrylate (MMA).
[0025] The following embodiments demonstrate the advantages of some granular fertilizers disclosed in the present invention by several studies. The embodiments are intended to enable one skilled in the art to understand the claimed features. The present inventions are neither limited to any single aspect nor embodiment thereof.
[0026] The first study is based on one embodiment of the present invention. In the study, the effects of different fertilizers on tomatoes cultivated in a screen house were investigated. Group A as control was treated with water in this study; Group B was treated with CaSCU granule; and Group C was treated with a granular fertilizer of the present invention. The granular fertilizer was manufactured by the following steps: first, molding or rolling CaSCL to form a CaSCU granule;
then, the surface additive, 2.2x107 CFU/g PSM, was homogeneously mixed with an oil, mineral oil, to form a solution; finally, sprayed the solution on the surface of CaSCfi granule to let the solution (i.e., the mineral oil with PSM dispersed within) cover the CaSCb granule to form a granular fertilizer.
[0027] In order to investigate the effect of different fertilizers on tomatoes, 3 blocks of CHUNG-HUA-TSAO soil (available at Taiwan Fertilizer Co., Ltd., characterized by that the N, P2O5, and K2O content is less than 1%) were allocated into Group A, B, and C. As previously described, the soil in Group A,
2019202785 18 Apr 2019
Group B, and Group C was fertilized with water, CaSCU granule, and the granular fertilizer respectively. Tomato seedlings were then transplanted in these three blocks. Two days after transplantation, each groups were administrated with additional 80 g of Lion-horse Nitrophoska Special (characterized by that N:P2Os:K2O = 12.0:12.0:17.0). Tomatoes were harvested after three weeks, and the weight, sweetness, and flower numbers were then measured.
[0028] The result of this study is described in the table below. From the aspect of total weight, Group C (treated with the granular fertilizer manufactured by a method disclosed in the present invention) has the highest total weight (3267 g), followed by Group B (1993 g) and Group A (659 g). The differences on the average sweetness and average flower numbers were not significant, but Group C does have the highest average sweetness (7.3°Bx) and average flower numbers (17 flowers).
Group Harvest Weight Sweetness Total Weight Avg. Sweetness Avg. Flower Num.
A 1 0g 0°Bx 659 g 7.05°Bx 11
2 0g 0°Bx
3 0g 0°Bx
4 203 g 7°Bx
5 152 g 7.1°Bx
6 154 g 7.1°Bx
7 150 g 7°Bx
B 1 150 g 7.6°Bx 1993 g 7.2°Bx 13
2 53 g 6°Bx
3 150 g 7.6°Bx
4 600 g 7.8°Bx
5 412 g 7.5°Bx
6 178 g 7°Bx
7 450 g 6.8°Bx
2019202785 18 Apr 2019
c 1 200 g 7.1°Bx 3267 g 7.3°Bx 17
2 232 g 7.3°Bx
3 800 g 7.1°Bx
4 657 g 7.7°Bx
5 456 g 7.5°Bx
6 322 g 7.3°Bx
7 600 g 7.1°Bx
[0029] Accordingly, this study demonstrates that the granular fertilizer manufactured by the method can significantly improve the weight of tomatoes, and, though not significant, elevate the average sweetness and average flower number.
[0030] The second study is based on multiple embodiments of the present invention. In the study, the methods to manufacture granular fertilizers are slightly varied as described in the table below.
Fertilizer A B C D E F
Form of Product Form Granular Granular Granular Granular Powder (Instant) Powder (Instant)
Material NPK (11:5.5:22) 40% SOC NPK (15:10:8) 50% SOC NPK (3:3:3) 60% SOC NPK (3:3:3) NPK (3:5:5) NPK (2.5:5.5:2.5)
Surface Additive Bacillus mycoides AGB01 Bacillus safensis Bacillus safensis Bacillus safensis Bacillus safensis Bacillus mycoides AGB01
kg per Package 20 20 20 1 1 or 5 1 or 5
Conditions pH 6.8 6.8 6.92 8.36 - -
Drying Temp. (°C) 100 100 110 70-80 - -
Drying Time 15 15 10 20-30 - -
2019202785 18 Apr 2019
(min)
Final Product pH 6.3 6.9 6.8 8.2 6.0 6.1
EC (mS/cm) 52-83 29-45 30-58 13-28 28-40 35-47
CFU/g 107 107 107 107 109 109
[0031] The material used to produce Fertilizer A is an NPK fertilizer (N^CUIGO = 11.0:5.5:22.0) containing 40% soil organic matter (SOC). The NPK fertilizer is first molding or rolling into a granule A, and then a solution (Bacillus mycoides AGB01 dispersed in mineral oil) is sprayed on the surface of the granule A.
Finally, the granule A is dried under 100°C to form a granular fertilizer A (i.e., Fertilizer A).
[0032] The material used to produce Fertilizer B is an NPK fertilizer (N:P2Os:K2O = 15.0:18.0:10.0) containing 50% SOC. The NPK fertilizer is first molding or rolling into a granule B, and then a solution (Bacillus safensis dispersed in mineral oil) is sprayed on the surface of the granule B. Finally, the granule B is dried under 100°C to form a granular fertilizer B (i.e., Fertilizer B).
[0033] The material used to produce Fertilizer C is an NPK fertilizer (N:P2Os:K2O = 3.0:3.0:3.0) containing 60% SOC. The NPK fertilizer is first molding or rolling into a granule C, and then a solution (Bacillus safensis dispersed in mineral oil) is sprayed on the surface of the granule C. Finally, the granule C is dried under 110°C to form a granular fertilizer C (i.e., Fertilizer C).
[0034] The material used to produce Fertilizer D is an NPK fertilizer (N:P2Os:K2O = 3.0:3.0:3.0). The NPK fertilizer is first molding or rolling into a granule D, and then a solution (Bacillus safensis dispersed in mineral oil) is sprayed on the
2019202785 18 Apr 2019 surface of the granule D. Finally, the granule D is dried under 70-80°C to form a granular fertilizer D (i.e., Fertilizer D).
[0035] The material used to produce Fertilizer E is an NPK fertilizer (N^CUIGO = 3.0:5.0:5.0). The NPK fertilizer is first molding or rolling into a granule E, and then a solution (Bacillus safensis dispersed in mineral oil) is sprayed on the surface of the granule E. As such, a granular fertilizer E (i.e., Fertilizer E) is formed.
[0036] The material used to produce Fertilizer F is an NPK fertilizer (N:P2Os:K2O = 2.5:5.5:5.5). The NPK fertilizer is first molding or rolling into a granule F, and then a solution (Bacillus mycoides AGB01 dispersed in mineral oil) is sprayed on the surface of the granule F. As such, a granular fertilizer F (i.e., Fertilizer F) is formed.
[0037] The third study is based on some other embodiments of the present invention. In the study, the effects of different fertilizers, produced by the methods disclosed in the present invention with a commercial fertilizer as the material, on Ipomoea aquatica were investigated. Group A as control was treated with water in this study; Group B was treated with a granule molded from 1/2 HSIN-YANG-FENG Fertilizer B (N:P2O5:K2O = 13.0:5.0:7.0 and containing 15% SOC) as the material; Group C was treated with a granule molded from HSIN-YANG-FENG Fertilizer B as the material; Group D was treated with a granular fertilizer using HSIN-YANG-FENG Fertilizer B as the material for granule, in which a solution containing PSM dispersed in mineral oil is sprayed on the surface of the granule; and Group E was treated with a granular fertilizer using HSIN-YANG-FENG Fertilizer B and CaSCU as the material for granule, in which a solution containing PSM dispersed in mineral oil is sprayed on the io
2019202785 18 Apr 2019 surface of the granule.
[0038] The results of the study are recorded in the table below. From the aspect of weight, Group E has the highest weight (111.2 g) followed by Group D (80.8 g). Compared to Group C, the weights of Group E and Group D are 73.8% and 41.9% higher.
[0039] Accordingly, this study demonstrates that granular fertilizers manufactured by the methods disclosed in the present invention can significantly improve the weight of Ipomoea aquatica.
Group A B C D E
Treatment BK 1/2B B PSBB BCaPSB
Weight (g) 44.4 49.6 64.0 80.8 111.2
Percentage (%) -30.6 -22.5 0 (basal line) 41.9 73.8
Number of Leaf 23.4 30.2 31.2 29.6 39.2
[0040] The forth study is based on some other embodiments of the present invention. In the study, the effects of different fertilizers, produced by the methods disclosed in the present invention with another commercial fertilizer as the material, on Ipomoea aquatica were investigated. Group A as control was treated with water in this study; Group B was treated with a granule molded from 1/2 HSIN-YANG-FENG Fertilizer A (N:P2O5:K2O = 12.0:8.0:10.0 and containing 20% SOC) as the material; Group C was treated with a granule molded from HSIN-YANG-FENG Fertilizer A as the material; Group D was treated with a granular fertilizer using HSIN-YANG-FENG Fertilizer A as the material for granule, in which a solution containing PSM dispersed in mineral oil is sprayed on the surface of the granule; and Group E was treated with a granular fertilizer using HSIN-YANG-FENG Fertilizer A and CaSCU as the material for granule, in which a solution containing PSM dispersed in mineral oil is sprayed
2019202785 18 Apr 2019 on the surface of the granule.
[0041] The results of the study are recorded in the table below. From the aspect of weight, Group E has the highest weight (126.4 g) followed by Group D (116.4 g). Compared to Group C, the weights of Group E and Group D are 31.1% and 20.7% higher.
[0042] Accordingly, this study demonstrates that granular fertilizers manufactured by the methods disclosed in the present invention can significantly improve the weight of Ipomoea aquatica.
Group A B C D E
Treatment BK 1/2 A A PSBA ACaPSB
Weight (g) 44.4 68.0 96.4 116.4 126.4
Percentage (%) -53.9 -29.5 0 (basal line) 20.7 31.1
Number of Leaf 23.4 28.6 34.8 38.6 40.2
[0043] There are many inventions described and illustrated above. The present inventions are neither limited to any single aspect nor embodiment thereof, nor to any combinations and/or permutations of such aspects and/or embodiments. For the sake of brevity, many of those permutations and combinations will not be discussed separately herein.

Claims (20)

1. A granular fertilizer, comprising:
a granule; and a layer on the surface of the granule, wherein the layer comprises an oil and at least one surface additive dispersed in the oil.
2. The granular fertilizer as claimed in claim 1, wherein the granular fertilizer comprises an additive.
3. The granular fertilizer as claimed in claim 2, wherein the granule is made of one selected from the group consisting of urea, peat soil, kaolinite, clay, bentonite, diatomaceous earth, acid clay, and the combination thereof.
4. The granular fertilizer as claimed in claim 2, wherein the oil is one selected from the group consisting of mineral oil, base oil, lubricating oil, palm oil, soybean oil, and the combination thereof.
5. The granular fertilizer as claimed in claim 2, wherein the at least one surface additive is a bacterial powder.
6. The granular fertilizer as claimed in claim 5, wherein the bacterial powder comprises one selected from the group consisting of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mycoides AGB01, Bacillus subtilis, and the combination thereof.
7. The granular fertilizer as claimed in claim 6, wherein the 16S rDNA of Bacillus amyloliquefaciens comprises a nucleic acid sequence of SEQ ID No. 1, the 16S rDNA of Bacillus mycoides AGB01 comprises a nucleic acid sequence of SEQ ID No. 2, and the 16S rDNA of Bacillus subtilis comprises a nucleic acid sequence of SEQ ID No.
3.
8. The granular fertilizer as claimed in claim 2, wherein the at least one surface
2019202785 18 Apr 2019 additive is one selected from the group consisting of calcium lignosulfonate, sodium lignosulfonate, biochar, humic acid, amino acid, trace element, and the combination thereof.
9. The granular fertilizer as claimed in claim 2, wherein the additive is one selected from the group consisting of adhesive, anticaking agent, biocide, antioxidant, reducing agent, surfactant, and the combination thereof.
10. A method of manufacturing a granular fertilizer, comprising:
molding or rolling a material to form a granule;
preparing a solution by dispersing at least one surface additive in an oil;
spraying the solution on the granule to form a layer on the surface of the granule; and drying the granule to form the granular fertilizer.
11. The method as claimed in claim 10, wherein an additive is mixed in the material in the step of molding or rolling.
12. The method as claimed in claim 11, wherein the particle size of the granule is not more than 325 mm.
13. The method as claimed in claim 11, where the material is one selected from the group consisting of urea, peat soil, kaolinite, clay, bentonite, diatomaceous earth, acid clay, and the combination thereof.
14. The method as claimed in claim 11, wherein the oil is one selected from the group consisting of mineral oil, base oil, lubricating oil, palm oil, soybean oil, and the combination thereof.
15. The method as claimed in claim 11, wherein the at least one surface additive is a bacterial powder.
16. The method as claimed in claim 15, wherein the bacterial powder comprises one selected from the group consisting of Bacillus licheniformis, Bacillus
2019202785 18 Apr 2019 amyloliquefaciens, Bacillus mycoides AGB01, Bacillus subtilis, and the combination thereof.
17. The method as claimed in claim 16, wherein the 16S rDNA of Bacillus amyloliquefaciens comprises a nucleic acid sequence of SEQ ID No. 1, the 16S rDNA of Bacillus mycoides AGB01 comprises a nucleic acid sequence of SEQ ID No. 2, and the 16S rDNA of Bacillus subtilis comprises a nucleic acid sequence of SEQ ID No.
3.
18. The method as claimed in claim 11, wherein the at least one surface additive is one selected from the group consisting of calcium lignosulfonate, sodium lignosulfonate, biochar, humic acid, amino acid, micronutrient, and the combination thereof.
19. The method as claimed in claim 15 or 18, wherein the particle size of the at least one surface additive is not more than 60 mesh.
20. The method as claimed in claim 11, wherein the additive is one selected from the group consisting of adhesive, anticaking agent, biocide, antioxidant, reducing agent, surfactant, and the combination thereof.
2019202785 18 Apr 2019
S01
S02
S03
S04
FIG. 1
2019202785 18 Apr 2019
S11
S12
S13
S14
FIG. 2
2019202785 18 Apr 2019
Sequence Listing <110> Advanced Green Biotechnology Inc.
<120> Granular Fertilizer and Method of Manufacturing Thereof <130>
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637 <210> 2 <211> 1468
2019202785 18 Apr 2019 <212> DNA <213> Bacillus mycoides AGB01 <220>
<221>
<222>
<400> 2 ggacgatggc ggcgtgccta tagatgcaag tcgagcgaat ggattgagag cttgctctca60 agaagttagc ggcggacggg tgagtaacac gtgggtaacc tgcccataag actgggataa120 ctccgggaaa ccggggctaa taccggataa tattttgaac tgcatggttc gaaattgaaa180 ggcggcttcg gctgtcactt atggatggac ccgcgtcgca ttagctagtt ggtgaggtaa240 cggctcacca aggcaacgat gcgtagccga cctgagaggg tgatcggcca cactgggact300 gagacacggc ccagactcct acgggaggca gcagtaggga atcttccgca atggacgaaa360 gtctgacgga gcaacggcgc gtgagtgatg aaggctttcg ggtcgtaaaa ctctgttgtt420 agggaagaac aagtgctagt tgaataagct ggcaccttga cggtacctaa ccagaaagcc480 acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt atccggaatt540 attgggcgta aagcgcgcgc aggtggtttc ttaagtccga tgtgatgtga aagcccacgg600 ctcaaccgtg gagggtcatt ggaaactggg agacttgagt gcagaagagg aaagtggaat660 tccatgtgta gcggtgaaat gcgtagagat atggaggaaa caccagtggc gaaggcgact720 ttctggtctg taactgacac tgaggcgcga aagcgtgggg agcaaacagg attagatacc780 ctggtagtcc aggccgtaaa cgatgagtgc taagtgttag agggtttccg ccctttagtg840 ctgaagttaa cgcattaagc actccgcctg gggagtacgg ccggaaggct gaaactcaaa900 ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa960 gaaccttacc aggtcttgac atcctctgaa aactctagag atagagcttc tccttcggga1020 gcagagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt1080 ccggcaacga gcgcaaccct tgatcttagt tgccatcatt aagttgggca ctctaaggtg1140 actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga1200 cctgggctac acacgtgcta caatggacgg tacaaagagc tgcaagaccg cgaggtggag1260 ctaatctcat aaaaccgttc tcagttcgga ttgtaggctg caactcgcct acatgaagct1320 ggaatcgcta gtaatcgcgg atcagcatgc cgcggtgaat acgttcccgg gccttgtaca1380
2019202785 18 Apr 2019 caccgcccgt cacaccacga gagtttgtaa aggccagccg cctaaggtga acacgaga <210> 3 <211> 630 <212> DNA <213> Bacillus subtilis <220>
<221>
<222>
<400> 3 cgccgcgtga gtgatgaagg ttttcggatc accgttcgaa tagggcggta ccttgacggt gccagcagcc gcggtaatac gtaggtggca gctcgcaggc ggtttcttaa gtctgatgtg tggaaactgg ggaacttgag tgcagaagag tgcgtagaga tgtggaggaa caccagtggc tgaggagcga aagcgtgggg agcgaacagg cgatgagtgc taagtgttag ggggtttccg actccgcctg gggagtacgg tcgcaagact caagcggtgg agcatgtggt ttaattcgaa atcctctgac aatcctagag ataggacgtc cacccgaagt cggtggggta acctttatgg1440
1468 gtaaagctct gttgttaggg aagaacaagt60 acctaaccag aaagccacgg ctaactacgt120 agcgttgtcc ggaattattg ggcgtaaagg180 aaagcccccg gctcaaccgg ggagggtcat240 gagagtggaa ttccacgtgt agcggtgaaa300 gaaggcgact ctctggtctg taactgacgc360 attagatacc ctggtagtcc acgccgtaaa420 ccccttagtg ctgcagctaa cgcattaagc480 gaaactcaaa ggaattgacg ggggcccgca540 gcaacgcgaa gaaccttacc aggtcttgac600
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