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AU2017246730A1 - Synergistic combination of a lenacil compound and fludioxonil for dry film protection - Google Patents

Synergistic combination of a lenacil compound and fludioxonil for dry film protection Download PDF

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Publication number
AU2017246730A1
AU2017246730A1 AU2017246730A AU2017246730A AU2017246730A1 AU 2017246730 A1 AU2017246730 A1 AU 2017246730A1 AU 2017246730 A AU2017246730 A AU 2017246730A AU 2017246730 A AU2017246730 A AU 2017246730A AU 2017246730 A1 AU2017246730 A1 AU 2017246730A1
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AU
Australia
Prior art keywords
fludioxonil
lenacil
antimicrobial
coating composition
dry film
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2017246730A
Inventor
Pierre Marie Lenoir
Lukas Thomas Johannes VILLIGER
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Dow Global Technologies LLC
Original Assignee
Dow Global Technologies LLC
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Filing date
Publication date
Application filed by Dow Global Technologies LLC filed Critical Dow Global Technologies LLC
Publication of AU2017246730A1 publication Critical patent/AU2017246730A1/en
Abandoned legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Paints Or Removers (AREA)

Abstract

A synergistic antimicrobial composition containing lenacil and fludioxonil is provided. Also provided is a method of inhibiting the growth of or controlling the growth of microorganisms in a building material, a coating composition containing such a synergistic antimicrobial composition, and a dry film made from such a coating composition.

Description

This invention relates to combinations of antimicrobial compounds and their uses in dry film protection applications, the combinations having unexpectedly greater activity than would be expected for the use of both of the individual antimicrobial compounds.
[0002] Use of combinations of at least two antimicrobial compounds can broaden potential markets, reduce use concentrations and costs, and reduce waste. In some cases, commercial antimicrobial compounds cannot provide effective control of microorganisms, even at high use concentrations, due to weak activity against certain types of microorganisms,
e.g., those resistant to some antimicrobial compounds. Combinations of different antimicrobial compounds are sometimes used to provide overall control of microorganisms in a particular end user environment. For example, WO 1998/121962 discloses combinations of
3-iodo-2-propynyl-butylcarbamate and pyrithione, but this reference does not suggest any of the combinations claimed herein. Moreover, there is a need for additional combinations of antimicrobial compounds with relatively low impact on health and/or the environment. The problem addressed by this invention is to provide such additional combinations of antimicrobial compounds.
[0003] Antimicrobial compounds are sometimes included in liquid coating compositions that are applied to a substrate and that become dry films. It is desirable that such dry films control surface fungi and algae and that such dry films also present as little adverse effect as possible on health and the environment.
[0004] In the present invention there is provided a synergistic antimicrobial composition
Comprising 3-Cyclohexyl-6,7-dihydro-lH-cyclopentapyrimidine-2,4-(3/7,5H)-dione hereinafter, “lenacil” (CAS registry number 2164-08-1) and 4-(2,2-Difluoro-l,3-benzodioxol1
WO 2017/176544
PCT/US2017/024954
4-yl)-1 H-pyrrole-3-carbonitrile hereinafter “fludioxonil” (CAS registry number is 13134186-1).
[0005] The invention further provides a method of inhibiting the growth of or controlling the growth of microorganisms in a building material, the method comprising the step of adding a synergistic antimicrobial composition comprising lenacil and fludioxonil; wherein the weight ratio of the fludioxonil to lenacil is from 1:10 to 5:1.
[0006] The present invention further comprises a coating composition comprising a synergistic antimicrobial composition comprising lenacil and fludioxonil. The coating composition of the present invention may also comprise a synergistic antimicrobial composition comprising lenacil and fludioxonil; wherein the weight ratio of the fludioxonil to lenacil is from 1:10 to 5:1.
[0007] Lastly, the present invention provides a dry film made by a process comprising applying a layer of the coating composition onto a substrate comprising a synergistic antimicrobial composition comprising lenacil and fludioxonil; wherein the weight ratio of the fludioxonil to lenacil is from 1:10 to 5:1 to a substrate and drying the coating composition or allowing the coating composition to dry.
[0008] The following is a detailed description of the invention.
[0009] As used herein, the following terms have the designated definitions, unless the context clearly indicates otherwise.
[0010] The term antimicrobial compound refers to a compound capable of inhibiting the growth of or controlling the growth of microorganisms; antimicrobial compounds include bactericides, bacteriostats, fungicides, fungistats, algaecides, algistats, and herbicides depending on the dose level applied, system conditions and the level of microbial control desired. Such term “antimicrobial compound” as used herein is synonymous with the term “biocide”.
WO 2017/176544
PCT/US2017/024954 [0011] The term microorganism includes, for example, fungi (such as yeast and mold), bacteria and algae.
[0012] The following abbreviations are used throughout the specification: ppm = parts per million by weight (weight/weight), mL = milliliter, ATCC = American Type Culture
Collection.
[0013] Unless otherwise specified, temperatures are in degrees centigrade (°C), and references to percentages are by weight (wt%). Percentages of antimicrobial compounds in the composition of this invention are based on the total weight of active ingredients in the composition, i.e., the antimicrobial compounds themselves, exclusive of any amounts of solvents, carriers, dispersants, stabilizers or other materials which may be present.
[0014] As used herein, fludioxonil is fludioxonil (CAS registry number 131341-86-1).
[0015] When a ratio is the herein to be X:l or higher, it is meant that the ratio is Y:l, where
Y is X or greater, and when a ratio is the herein to be X: 1 or lower, it is meant that the ratio is Z: 1, where Z is X or less. The same logic follows for ratios that are “1 :X or higher” and
1:X or lower.
[0016] Lenacil belongs to the class of uracil herbicides, 3-cyclohexyl-l,5,6,7tetrahydrocyclopentapyrimidine-2,4(377)-dione (C13H18N2O2) (CAS registry number 2164-081). Lenacil is a known herbicide that is approved in many jurisdictions as a soil-acting herbicide for the control of grass and broad-leaved weeds in beet and other crops.
[0017] Lenacil has relatively low solubility in water. This relatively low solubility in water is preferred for an antimicrobial material that may be included in a coating composition or other building material, because dried coatings and building materials are exposed to water, which could tend to remove a highly soluble compound from the dried coating or the building material.
WO 2017/176544
PCT/US2017/024954 [0018] The present invention involves a composition that contains both lenacil and fludioxonil. It has been surprisingly found that such a composition is synergistically effective as a biocide. It has been especially surprisingly found that compositions that contain both lenacil and fludioxonil are synergistically effective as biocides against algae.
[0019] When fludioxonil is present, preferably the weight ratio of fludioxonil to lenacil compound is preferably 1:10 to 5:1.
[0020] The mixture of lenacil and fludioxonil may be included in a coating composition.
Lenacil and fludioxonil may be added to the coating composition separately or as a mixture or any combination thereof. Preferred coating compositions are liquid. Coating compositions may be aqueous or non-aqueous. Aqueous coating compositions generally contain 30% or more water by weight of the mixture, based on the weight of the coating composition.
[0021] Among embodiments in which lenacil and fludioxonil are included in paint or other coating composition, preferred coating compositions are liquid compositions, especially compositions that contain dispersions of polymers in aqueous media.
[0022] In addition to paints and other coating compositions such as marine anti-fouling, the antimicrobial compound combinations of the present invention are particularly useful in preservation of building materials, e.g., adhesives, caulk, joint compound, sealant, wallboard, etc., polymers, plastics, synthetic and natural rubber, paper products, fiberglass sheets, insulation, exterior insulating finishing systems, roofing and flooring felts, building plasters, bricks, mortar, gypsum board, wood products and wood-plastic composites. When an antimicrobial compound combination of the present invention is present in a building material, it is preferred that some or all of the antimicrobial compound combination is present at the surface of the building material or near enough to the surface of the building material to inhibit microbial growth on that surface.
WO 2017/176544
PCT/US2017/024954 [0023] In some embodiments, latex paints or other liquid coating compositions are used that contain the antimicrobial compound combinations disclosed herein.
[0024] Coating compositions are designed so that a layer of the coating composition can readily be applied to a substrate and then dried or allowed to dry to form a dry film. Coating compositions contain at least one binder. Binders contain one or more of the following: one or more polymer, one or more oligomer, and/or one or more monomer. Oligomers and monomers in binders are designed to polymerize and/or crosslink during or after the formation of the dry film. Polymers in a binder may or may not be designed to crosslink during or after the formation of the dry film.
[0025] Coating compositions optionally contain one or more pigment. A pigment is a mineral or an organic substance in the form of small solid particles. Pigments provide full or partial opacity to the dry film.
[0026] The antimicrobial compound combinations are useful for preservation of the dry film coating resulting after application of paint or other liquid coating composition. Preferably, the antimicrobial composition is an aqueous latex paint comprising one or more of the antimicrobial compound combinations disclosed herein, or the dry film coating resulting from application of the paint to a surface. An aqueous latex paint is an aqueous liquid coating composition in which the binder is a polymer in the form of a latex (i.e., in the form of polymer particles dispersed throughout the water). More preferred are aqueous latex paints in which the binder contains one or more acrylic polymer.
[0027] Typically, the amount of the antimicrobial compound combinations of the present invention to control the growth of microorganisms is from 100 ppm to 40,000 ppm active ingredient. For example, in the present invention, lenacil plus fludioxonil is present in an amount from 100 ppm to 10,000 ppm. The antimicrobial combinations of the composition are present in an amount of at least 100 ppm and no more than 10,000 ppm, alternatively no
WO 2017/176544
PCT/US2017/024954 more than 8,000 ppm, alternatively no more than 6,000 ppm, alternatively no more than
5,000 ppm, alternatively no more than 3,000 ppm, alternatively no more than 2500 ppm, alternatively no more than 2,000 ppm, alternatively no more than 1,000 ppm, and alternatively no more than 500 ppm. Concentrations mentioned above are in a liquid coating composition containing the antimicrobial compound combinations; antimicrobial compound combination levels in the dry film coating will be higher.
[0028] The present invention also encompasses a method for preventing microbial growth in building materials, especially in dry film coatings, by incorporating any of the claimed antimicrobial compound combinations into the materials.
[0029] Typically, the antimicrobial compositions are used to inhibit growth of algae and/or fungi.
[0030] The composition of the present invention contains lenacil and fludioxonil. It is contemplated that some embodiments may contain one or more additional antimicrobial compound.
[0031] The following are examples of the present invention.
[0032] Sample preparation for antimicrobial testing was performed as follows:
[0033] Sample preparation:
[0034] A powder containing 100% fludioxonil and a dispersion containing 30% biocide active ingredient for lenacil was post added into white, acrylic/silicone based outdoor paint free of biocides to give a total active ingredient concentration of 10,000 and l,000ppm respectively. These paints were then diluted with a biocide free acrylic/silicone based paint and mixed to prepare targeted concentrations of biocide combinations for the testing. The total biocides concentrations obtained were 125, 250, 500, 1000, 2000 and 5000 ppm. After biocides addition or dilution, the paints were mixed 90 seconds with the horse power shaker (AXEL 75M3372/ Agitateur SO-10MI) until uniformity was achieved. The obtained paints
WO 2017/176544
PCT/US2017/024954 containing fludioxonil and containing lenacil were mixed together to obtain paints containing the two actives in the desired ratios and amounts. After one day, the paints were applied to
Schleicher & Schuell filter paper at 280pm wet film thickness and dried for 3 days at room temperature avoiding direct exposure to sunlight. Square discs (1.8 cm x 1.8 cm) were cut out from each panel and were used as the substrate for algal efficacy tests. This sample size allowed for an agar border when the sample disc was placed into the well of the test plate.
[0035] Algal Efficacy Testing:
[0036] Algal efficacy was tested according to modified ASTM 5589 which is a standard accelerated test method for determining resistance of various coatings (including paints) to algal defacement. To accommodate for high-throughput screening, this method was scaled down from petri plates to 6-well plates. Bold Modified Basal Freshwater Nutrient Solution was used as growth medium for the algal culture and Bold’s agar was used for the efficacy testing. A single coupon was placed with a pair of sterile forceps at the center of the agar plug (on top) with the painted surface facing upwards. The algal inoculum was prepared by diluting an exponentially growing culture of Chlorella sp. to lx 106 cfu/ml.
WO 2017/176544
PCT/US2017/024954 [0037] Algal inoculum:
Organism Strain No. Type Medium for testing
Chlorella sp. ATCC7516 Unicellular Chlorophyte Bold Modified Freshwater Solution
[0038] Each well that contains a tested coupon was inoculated with 1750 μΐ of algal suspension (lx 106 cfu/ml) making sure that the whole surface (paint film as well as the agar surrounding it) was evenly covered. The plates were incubated at room temp (21 °C - 25 °C) with cyclic exposure to 14 hours fluorescent light followed by 10 hours darkness, for a period of three weeks.
[0039] At the end of the incubation period the samples were scored for percent inhibition regarding color intensity compared to the blank sample [0040] The Synergy Index calculation was performed as follows:
[0041] The SI is calculated based on F.C.Kull et. Al. method (Applied Microbiology, Vol. 9 (1961). In this study, SI was calculated based on the following formula with the minimum inhibitory concentration chosen based on the percent inhibitory exhibited by the individual antimicrobial against each microorganism tested.
SI= Qa/QA+Qb/QB
Qa = the concentration of Antimicrobial A in the blend
QA = the concentration of Antimicrobial A as the only biocide
WO 2017/176544
PCT/US2017/024954
Qb = the concentration of Antimicrobial B in the blend
QB = the concentration of Antimicrobial B as the only antimicrobial [0042] SI value of < 1 in the formula indicates a synergism of the blended biocides exists.
[0043] Note: If any of the active with maximum concentration tested did not exhibit some inhibition, this maximum tested concentration is used to calculate the estimated SI and a sign of less than (<) is included to take into account that higher concentration of the active (e.g.
Lenacil) is needed to achieve the targeted inhibition. The minimal targeted inhibition was set at 75%, meaning a coupon with at least 75% algal growth inhibition was considered as a pass.
[0044] Compositions listed below that contain both lenacil and fludioxonil are examples of the present invention.
[0045] Test Results for fludioxonil with lenacil at three weeks were as follows:
[0046] Pass level >75% inhibition
Pass level >75% inhibition
Fludioxonil/ Lenacil
Chlorella
Fludioxonil
Total cone, ppm 5000
10
Lenacil
Total cone, ppm 1,000
% inhibition 90
Fludioxonil/Lenacil 1/10
Total cone, ppm 1000
WO 2017/176544
PCT/US2017/024954
% inhibition 100
SI <0.93
Fludioxonil/Lenacil 1/5
Total cone, ppm 250
% inhibition 80
SI <0.22
Fludioxonil/Lenacil 1/2
Total cone, ppm 250
% inhibition 75
SI <0.18
Fludioxonil/Lenacil 1/1
Total cone, ppm 250
% inhibition 80
SI <0.15
Fludioxonil/Lenacil 2/1
Total cone, ppm 250
% inhibition 80
SI <0.12
Fludioxonil/Lenacil 5/1
Total cone, ppm 500
% inhibition 80
SI <0.17
[0047] Fludioxonil + Lenacil at ratios of 1:10 to 5:1 exhibited a synergy.
WO 2017/176544
PCT/US2017/024954

Claims (8)

1. A synergistic antimicrobial composition comprising lenacil and fludioxonil.
2. The synergistic antimicrobial composition of claim 1, wherein the weight ratio of the fludioxonil to lenacil is from 1:10 to 5:1.
3. A method of inhibiting the growth of or controlling the growth of microorganisms in a building material, the method comprising the step of adding the synergistic antimicrobial composition of claim 1 to the building material.
4 A method of inhibiting the growth of or controlling the growth of microorganisms in a building material, the method comprising the step of adding the synergistic antimicrobial composition of claim 2 to the building material.
5. A coating composition comprising the synergistic antimicrobial composition of claim 1.
6. A coating composition comprising the synergistic antimicrobial composition of claim 2.
7. A dry film made by a process comprising applying a layer of the coating composition of claim 5 to a substrate and drying the coating composition or allowing the coating composition to dry.
8. A dry film made by a process comprising applying a layer of the coating composition of claim 6 to a substrate and drying the coating composition or allowing the coating composition to dry.
AU2017246730A 2016-04-04 2017-03-30 Synergistic combination of a lenacil compound and fludioxonil for dry film protection Abandoned AU2017246730A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662317733P 2016-04-04 2016-04-04
US62/317,733 2016-04-04
PCT/US2017/024954 WO2017176544A1 (en) 2016-04-04 2017-03-30 Synergistic combination of a lenacil compound and fludioxonil for dry film protection

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AU2017246730A1 true AU2017246730A1 (en) 2018-11-08

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EP (1) EP3439475A1 (en)
CN (1) CN108882707A (en)
AU (1) AU2017246730A1 (en)
BR (1) BR112018069692A2 (en)
MX (1) MX2018012040A (en)
WO (1) WO2017176544A1 (en)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3836332A1 (en) * 1988-10-25 1990-04-26 Richter Gedeon Vegyeszet USE OF 3- (CYCLOHEXYL) -5,6- (TRIMETHYLENE) -URACIL AND / OR 3- (TERT.-BUTYL) -5- (CHLORO) -6- (METHYL) -URACIL FOR CHANGING THE BIOLOGICAL WATER QUALITY OF FREE WAITERS
AU5448998A (en) 1996-11-20 1998-06-10 Troy Corporation Synergistic algaecide
JP5432925B2 (en) * 2008-02-06 2014-03-05 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ Combination of phenylpyrrole and pillion compounds
EP2925133B1 (en) * 2012-11-30 2016-11-02 Rohm and Haas Company Synergistic combination of lenacil and terbutryn for dry film protection
US9301528B2 (en) * 2012-11-30 2016-04-05 Rohm And Haas Company Synergistic combination of a lenacil compound and one of DCOIT or OIT for dry film protection
US9288991B2 (en) * 2012-11-30 2016-03-22 Rohm And Haas Company Synergistic combination of a lenacil compound and zinc pyrithione for dry film protection
KR20150091088A (en) * 2012-11-30 2015-08-07 롬 앤드 하스 캄파니 Synergistic combination of lenacil and IPBC for dry film protection
WO2015031874A1 (en) * 2013-08-30 2015-03-05 Rohm And Haas Company Synergistic combination of a lenacil compound and carbendazim for dry film protection
AU2014311984B2 (en) * 2013-08-30 2017-09-21 Dow Global Technologies Llc Synergistic combination of a lenacil compound and flurochloridone for dry film protection
KR20160052580A (en) * 2013-08-30 2016-05-12 롬 앤드 하아스 컴패니 Synergistic combination of a lenacil compound and phenylurea herbicides for dry film protection

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MX2018012040A (en) 2019-01-14
CN108882707A (en) 2018-11-23
EP3439475A1 (en) 2019-02-13
WO2017176544A1 (en) 2017-10-12
BR112018069692A2 (en) 2019-01-29

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