AU2012211390B2 - Metallo-beta-lactamase inhibitors - Google Patents

Abstract

Abstract A new metallo--lactamase inhibitor which acts as a medicament for inhibiting the inactivation of P-lactam antibiotics and recovering anti-bacterial activities is disclosed. The maleic acid derivatives having the general formula (I) have 5 metallo-p-lactamase inhibiting activities. It is possible to recover the anti-bacterial activities of P-lactam antibiotics against metallo- P-lactamase producing bacteria by combining the compound of the general formula (I) with P-lactam antibiotics. __ (I) M'OOC COOM'

Description

Australian Patents Act 1990 - Regulation 3.2A ORIGINAL COMPLETE SPECIFICATION STANDARD PATENT Invention Title "Metallo-beta-lactamase inhibitors" The following statement is a full description of this invention, including the best method of performing it known to us:- C \NRPonb\DCOCDAR\45 1249 I DOC-NA91/21'12 Metallo-p-lactamase inhibitors This application is a divisional of Australian Patent Application No. 2006293023, the entire content of which is incorporated herein by reference. [Background of the Invention] [0001] Field of the Invention The present invention relates to a metallo-p-lactannase inhibitor comprising a maleic acid compound as an effective ingredient, and more particularly, it relates to a pharmaceutical composition to improve the effectiveness against metal lo-p-lactamase producing resistant bacteria in combination with p-lactam antibiotics for the therapy of bacterial infection in animal or human being and a method for treating the bacterial infections. [0002] Background Art P-lactamase plays an important role in the acquisition of resistance against p-lactam antibiotics in bacteria. Particularly, metallo-p-lactamases which contains zinc at their active center and exhibits wide ranges of substrate specificities are at issue due to the fact that the metallo--lactamases also hydrolyze carbapenem antibiotics which are relatively stable to serine-p-lactamase. In fact, metallo-p-lactamase producing bacteria are a menace to clinically important p-lactam pharmaceuticals due to the acquisition of resistance to such pharmaceuticals. Metallo-p-lactamases have been confirmed in a variety of bacterial strains such as Bacillus cereus, Bacteriocides fragilis. Escherichia coli, Aeromonas hydrophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, Stenotrophomonas maltophilia, Shigella flexneri, Alcaligenes xylosoxidans, Legionella gormanii, Chryseobacterium meningosepticum, Chryseobacterium indologenes, Acinetobacter baumannii, Citrobacter freundii, and Enterobacter cloacae. Particularly, Pseudomonas aeruginosa causes serious problem because of its conspicuous multidrug resistance. Clavulanic acid, sulbactam, and tazobactam which are now used as p-lactamase inhibitors are effective against serine-p-lactamase having serine as an active center, but these drugs exhibit not inhibiting effect on metallo-0-lactamases. [0003] Therefore, metallo-0-lactamase inhibitors have been 5 increasingly necessitated in order to recover the effectiveness of P-lactam antibiotics such as imipenem against metallo-0-lactamase producing resistant bacteria. [0004] Since a metallo-1P-lactamase coded on a transferable plasmid 10 has first been reported in Pseudomonas aeruginosa, many compounds have been reported as those having metallo-p-lactamase inhibiting activities. In W098/17639, W097/30027, W098/40056, W098/39311, and W097/10225, certain p-thiopropionyl-amino acid derivatives have been 15 described together with their uses as the inhibitor against the metallo- -lactamases. Also, several literatures disclose thiols and thioesters as metallo-1P-lactamase inhibitors (Biol. Pharm. Bull. 1997, 20, 1136; FEMS Microbiology Letters 1997, 157, 171; Antimicrob. Agents Chemother. 1997, 41, 135; Chem. 20 Commun. 1998, 1609; Biochem. .. 1998, 331, 703; WOOO/076962). Furthermore, there are disclosed succinic acid compounds as the metallo- 0-lactamase inhibitors in WO01/030148 and WO01/030149. In addition, there is a literature in which general situations on various 25 metallo-0-lactamase inhibitive compounds and metallo- P-lactamase producing bacteria are described (Clin. Microbiol.Rev. 2005, 18, 306). However, none of the literatures described above disclose or suggest the maleic acid derivatives as the compounds of the present invention. 30 [0005] In order to exert the effect of the metallo-p0-lactamase inhibitor against the metallo-p-lactamase producing resistant bacteria actually in clinical practice, it is essential to recover the effectiveness in combination with p-lactam antibiotics. However, 35 there has hitherto been described scarcely such combination effect against bacterial species including Pseudomonas aeruginosa which is at issue in the medical field. There is at present no metallo-p-lactamase inhibitor which is effective on infections in human beings and animals. [0006] 5 With respect to the maleic acid derivatives, dimethyl maleate has been disclosed in Japanese Patent Laid-Open Publication No. 57-207245, dimethyl maleate and diethyl maleate, i.e. the compounds having the general formula (I) wherein M1 represents a hydrogen atom, and the like have been disclosed 10 in Acta Chem. Scand. 1964, 18 , 1276, and 3-methyl-2-tetradecanyl maleate has been disclosed in Japanese Patent Publication No. 7-91213. However, the salts or esters having a group which can be hydrolyzed in vivo of these derivatives have not been described in them. 15 [0007] Also, there is disclosed in Bioorg. Med. Chem., 2000, 8, 571 2-hydroxymethyl-3-tetradecanyl maleate but not the other 2-hydroxymethyl-3-lower alkyl maleate. Furthermore, there is disclosed in J. Am. Chem. Soc. 1955, 77, 6702 dicarboxylic 20 acids having a cyclic alkyl group in the side chain, which are mono-substituted fumarate derivatives but not maleate derivatives. There is disclosed in J. Org. Chem. 1984, 49, 1985 maleic acid derivatives, one of which has an alkylthio substituent, but the other has an alkenyl group. There are 25 disclosed in WO 91/08775 and Tetrahedron Lett. 1988, 29, 3869 maleic acid ester compounds mono-substituted with an alkoxy group, but a carboxylic acid thereof, i.e. the compound having the general formula (I) wherein M1 represents a hydrogen atom, or a salt thereof have not been disclosed in these literatures. In 30 addition, a compound having a dihydrofuranyl group has been described in Nord. Pulp Pap. Res. J. 1994, 9, 84, and a compound having a pyridinium group has been described in Angew. Chem., 1990, 102, 1164, but no metallo-@-lactamase inhibiting activity has been indicated in therein. 35 [Summary of the Invention] [0008] 4 Accordingly, the present invention advantageously provides a new metallo P-lactamase inhibitor which acts as a medicament for inhibiting the inactivation of p-lactam antibiotics and recovering anti-bacterial activities. [0009] 5 The present inventors have now found that maleate derivatives and pharmacologically acceptable salts thereof have an inhibitory effect against metallo-p-lactamases. Furthermore, it has been found that these compounds are effective for recovering the activities of P-lactam antibiotics against the metallo-@-lactamase producing bacteria. The present invention 10 is based on such findings. [0009a] According to one aspect of the present invention, there is provided a method of treating or preventing a bacterial infection comprising administering to a subject in need thereof a metallo-p-lactamase inhibitor, 15 which comprises a compound represented by the following formula (I), or a salt or solvate thereof: (I) M10OC COOMI in which R' represents a C 2

-

6 alkyl group, a C 3

-

7 cycloalkyl group, a hydroxymethyl 20 group, a -C 1 3 alkylene-phenyl group, a -CO- 1 alkylene-heterocycle, a -O-C- 6 alkyl group, or a -S-C 1 s alkyl group, all of which may be substituted,

R

2 represents a C 1 6 alkyl group, a C 3

-

7 cycloalkyl group, a hydroxymethyl group, -C- 3 alkylene-phenyl group, a -Co-, alkylene-heterocycle, a -O-C, 1 6 alkyl group, or a -S-C 1 s alkyl group, all of which may be substituted, 25 each M independently represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which can be hydrolyzed in vivo. [0009b] W~lrrnIlCIOVC\NKI'OrIbl~L)CC\lKl(R1fV.939_ d ZIL/2//UI 4a According to another aspect, there is provided a compound represented by the following general formula (II), or a salt or solvate: M>OC COOM2

M

2 000 0M 5 in which

R

3 represents a C 2

-

6 alkyl group, a C 3

.

7 cycloalkyl group, or a hydroxymethyl group, all of which may be substituted

R

4 represents a C 1

.

6 alkyl group or a C 3

.

7 cycloalkyl group, all of which may 10 be substituted, each M 2 independently represents a sodium cation or a potassium cation. [0009c] According to a further aspect, there is provided a compound represented by the following general formula (III), or a salt or solvate thereof: 15 R5 R

M

3 00C COOM 3 in which

R

5 represents an ethyl group,

R

6 represents a C 1

-

3 linear alkyl group, the two groups M 3 , represent a 20 sodium cation or a potassium cation. [0009d] According to another aspect, there is provided a compound represented by the following general formula (IV), or a salt or solvate thereof: 4b

R

7 IR (IV)

M

4 00C COOM 4 in which

R

7 represents a C 1

_

6 alkyl group, a C3- 7 cycloalkyl group, a -C 1

-

3 alkylene phenyl group, a -C2 alkylene-ring A, a -O-C 1

-

6 alkyl group, or a -S-CJ- 6 alkyl 5 group, all of which may be substituted,

R

8 represents a -Cls 3 alkylene-phenyl group, a -Co.

1 alkylene-ring A, a -0

C

1 -6 alkyl group, or a -S-C.

6 alkyl group, all of which may be substituted, the ring A represents a five- to ten-membered mono- or bi-cyclic heterocyclic ring having 1-4 hetero atoms selected from nitrogen, oxygen 10 and sulfur atoms, each M 4 independently represents a sodium cation or a potassium cation, provided that when R' is a C 1

.

6 alkyl group, R 8 excludes dihydrofuran. [0010] According to another aspect of the present invention, there is provided a 15 process for treating bacterial infections, which comprises administering the compound represented by the following formula (I), or a salt or solvate thereof to mammals including human being: RI (I) M'OOC COOM' in which 20 R' represents a C 2

.

6 alkyl group, a C 3

.

7 cycloalkyl group, a hydroxymethyl group, a -Cs 3 alkylene-phenyl group, a -Co 1 -alkylene-heterocycle, a -0-Cl.- 6 alkyl group, or a -S-C 1

.

6 alkyl group, all of which may be substituted,

R

2 represents a C 1

-

6 alkyl group, a C 3

..

7 cycloalkyl group, a hydroxymethyl group, a -C 1 3 alkylene-phenyl group, a -Co_ 1 , alkylene-heterocycle, a -0-Cl- 6 25 alkyl group, or a -S-C 1

.

6 alkyl group, all of which may be substituted, 4c each M' independently represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which may be hydrolyzed in vivo. 1-Lactam antibiotics are in general administered concomitantly or 5 sequentially with the compound represented by the general formula (I), or a salt or solvate thereof. [0011] Also, according to another aspect of the present invention, there is provided a pharmaceutical composition comprising the 5 metallo-p0-lactamase inhibitor, the p-lactam antibiotics, and optionally a pharmaceutically acceptable carrier, [0012] Also, according to another aspect of the present invention, there is provided a process for treating bacterial infections, which 10 comprises co-administering a P-lactam antibiotic and a metallo-0-lactamase inhibitor defined above. [0013] In addition, according to another aspect of the present invention, there is provided a compound represented by the 15 following general formula (II), or a salt or solvate thereof: Ra R (II)

M

2 00C COOM 2 in which

R

3 represents a C 1

-

6 alkyl group of a C 3

-

7 cycloalkyl group, all of which may be substituted, provided that when R 3 is a C 1

-

2 linear 20 alkyl group, this has at least one substituent,

R

4 represents a C1- 6 alkyl group or a C 3

-

7 cycloalkyl group, all of which may be substituted, each M 2 independetnly represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically 25 acceptable group which may be hydrolyzed in vivo. [0014] Also, according to another aspect of the present invention, there is provided a compound represented by the following general formula (III), or a salt or solvate thereof: R5 R (III)

M

3 00C COOM 3 in which

R

5 represents an ethyl group,

R

6 represents a C 1

.

3 linear alkyl group, 5 each M 3 independently represents a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which may be hydrolyzed in vivo. [0015] Also, according to another aspect of the present invention, there 10 is provided a compound represented by the following general formula (IV), or a salt or solvate thereof: R 7 R8 (IV)

M

4 00C COOM 4 in which

R

7 represents a C 1

-

6 alkyl group, a C 3

-

7 cycloalkyl group, a -C 1

.

3 15 alkylene-phenyl group, a -C 1 alkylene-ring A, a -0-C 1

.

6 alkyl group, or a -S-C 1

.

6 alkyl group, all of which may be substituted,

R

8 represents a -C 1

.

3 alkylene-phenyl group, a -Co.

1 alkylene-ring A, a -0-C 1 .- alkyl group, or a -S-C 1

.

6 alkyl group, all of which may be substituted, 20 the ring A represents a five- to ten-membered mono- or bi-cyclic heterocyclic ring having 1-4 hetero atoms selected from nitrogen, oxygen and sulfur atoms, each M 4 independently represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically 25 acceptable group which may be hydrolyzed in vivo, provided that when R 7 is a C 1

.

6 alkyl group, R 8 excludes dihydrofuran. [0016] Also, according to another aspect of the present invention, there is provided a compound represented by the following general formula (V), or a salt or solvate thereof: R9 RI"

M

5 00C COOMS 5 in which

R

9 represents a C 1

-

6 alkyl group, a C 3

.

7 cycloalkyl group, a -C1-3 alkylene-phenyl group, a -Co- 1 alkylene-ring B, a -0-C1.

6 alkyl group, or, a -S-C 1

.

6 alkyl group, all of which may be substituted, Rl 0 represents a -C 1

.

3 alkylene-phenyl group, a -Co- 1 10 alkylene-ring B, a -0-C 1

-

6 alkyl group, or a -S-C 1

-

6 alkyl group, all of which may be substituted, the ring B represents pyridine, piperidine or tetrahydropyran, each M 5 independently represents a hydrogen atom, a pharmaceutically acceptable cation or a pharmaceutically 15 acceptable group which may be hydrolyzed in vivo, provided that the case when R 9 represents pyridinium is excluded. [Detailed Description of the Invention] [0017] 20 Definition Unless otherwise specified, the terms "C 1

.

6 ", "C 2

-

6 ", "C3 7 1 ,

"C

1 .31", "Co.1", "C 2

-

3 ", "C 1 ", and the like denote the number of carbon atoms, and for example, "a C 1

-

6 alkyl group" represents an alkyl group having 1-6 carbon atoms. Also, Co represents a 25 bond. Lower preferably means C 1

-

6 , and in the case of the cyclic group it preferably means C 3

-

7 . [0018] The term "halogen atom" represents a fluorine atom, a chlorine atom, a bromine atom, or an iodine atom. 30 [0019] The term "hetero atom" represents a nitrogen atom, an oxygen atom, or a sulfur atom.

[0020] As herein used, the terms "alkyl group" or "alkoxy group" as a group or as a part of a group preferably mean a linear or branched alkyl group having 1-6 carbon atoms or alkoxy group 5 having 1-6 carbon atoms. Examples of "alkyl" include methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, s-butyl, t-butyl, n-pentyl, neopentyl, i-pentyl, t-pentyl, n-hexyl, i-hexyl, and the like. Examples of "alkoxy" include methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, i-butoxy, s-butoxy, t-butoxy, n-pentyloxy, 10 neopentyloxy, i-pentyloxy, t-pentyloxy, n-hexyloxy, i-hexyloxy, and the like. Also, the term lower alkyl group preferably means a C 1

.

6 alkyl group, as defined above. [0021] As herein used, the term "cycloalkyl group" as a group or as a 15 part of a group preferably means a mono-cyclic alkyl group having 3-7 carbon atoms. Examples of "cycloalkyl" include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and the like. Also, the term lower cycloalkyl group preferably means a C 3

-

7 cycloalkyl group, as defined above. 20 [0022] As herein used, the term "alkylene" as a group or as a part of a group preferably means an alkylene group having 1-3 carbon atoms. Examples of "alkylene" include methylene, ethylene, propylene, and the like. 25 [0023] As herein used, the term "heterocyclic ring" includes a five- to fourteen-membered mono- to tri-cyclic heterocyclic ring containing 1-4 hetero atoms selected from nitrogen, oxygen and sulfur atoms, more preferably a five- to ten-membered mono 30 to bi-cyclic heterocyclic ring containing 1-4 hetero atoms selected from nitrogen, oxygen and sulfur atoms. Preferred examples include tetrahydrofuran, furan, pyrrolidine, piperidine, pyrazolidine, imidazolidine, piperazine, morpholine, thiomorpholine, pyrrole, thiophene, oxazole, isoxazole, thiazole, 35 isothiazole, imidazole, pyrazole, pyridine, pyridazine, pyrimidine, pyrazine, triazole, tetrazole, thiadiazole, azetidine, thiazoline, quinuclidine, triazine, isobenzofuran, indole, indolizine, chromene, quinoline, isoquinoline, cinnoline, quinazoline, quinoxaline, phthalazine, purine, pteridine, and the like. 5 [0024] The term "aryl" means preferably aromatic rings such as phenyl, substituted phenyl, and the like as well as fused rings such as naphthyl, phenanthrenyl, fluorenyl, anthryl, and the like. Preferred aryl groups include a phenyl group, a naphthyl group 10 and a fluorenyl group. [0025] As herein used, the term "which may be substituted" means that which may be substituted preferably by 1-6 substituents, more preferably 1-3 substituents, and the term "substituents" 15 include a hydroxyl group, a thiol group, a C 1

.

6 alkyl group, a C 1

.

6 alkoxy group, a S-C1.

6 alkyl group, amino, a mono-substituted amino group, a di-substituted amino group, an amide group, a guanidyl group, an N-substituted amide group, an N,N-disubstituted amide group, a halogen atom, a carboxyl 20 group, a phenyl group, a substituted phenyl group, a C 1

.

6 alkylcarbonyl group, a heterocycle, a heterocyclic carbonyl group, and the like, and the phenyl group may be fused. Preferred substituted groups include a hydroxyl group, a C 1

.

6 alkyl group, a C1- 6 alkoxy group, an amino group, a 25 mono-substituted amino group, a di-substituted amino group, an amide group, a guanidyl group, an N-substituted amide group, an N,N-disubstituted amide group, a halogen atom, a carboxyl group, a phenyl group, which may be fused, a substituted phenyl group, a C 1

-

6 alkylcarbonyl group, a 30 heterocycle, and a heterocyclic carbonyl group. [0026] The "substituents" described above including a C 1

.

6 alkyl group, a C 1

-

6 alkoxy group, an S-C 1

-

6 alkyl group, and a C 1

.

6 alkylcarbonyl group, and an alkyl group and an alkoxy group as 35 a part of a group have the same meanings as described above. Also, a halogen atom has the same meaning as described above. The C 1

.

6 alkyl group, the C 1

.

6 alkoxy group, the S-C 1 alkyl group and the C 1

.

6 alkylcarbonyl group may be further substituted by the "substituents" described above. Above all, the C 1

.

6 alkyl group and the C 1

.

6 alkoxy group may be 5 substituted by a hydroxyl group, an amino group, a mono-substituted amino group, a di-substituted amino group, an amide group, a guanidyl group, an N-substituted amide group, an N,N-disubstituted amide group, a carboxyl group, a heterocycle, a phenyl group, a substituted phenyl group, and 10 the like. [0027] When the "substituents" described above is a carboxyl group or a group having a carboxyl group as a part of the group, the carboxyl group may be a pharmaceutically acceptable cation or 15 a pharmaceutically acceptable group which may be hydrolyzed in vivo, and preferably includes a sodium salt or a potassium salt. [0028] The term "substituted" in the mono-substituted amino group, 20 the di-substituted amino group, the amide group, the N-substituted amide group, the N,N-disubstituted amide group, the substituted phenyl group, and the like of the "substituents" described above means that these groups preferably have the "substituents" described above. 25 [0029] The heterocycle and the heterocycle in the heterocyclic carbonyl group of the "substituents" have the same meanings as the "heterocycle" described above. [0030] 30 Preferred examples of the heterocyclic carbonyl group include morpholylcarbonyl, piperazylcarbonyl, piperidylcarbonyl, and the like, preferably morpholyl-4-yl-carbonyl, piperazin-4-yl carbonyl, (4-hydroxypiperazin)-1-yl carbonyl, and the like. [0031] 35 Metallo-pD-lactamase inhibitor comprising the compound represented by the general formula (I) According to one aspect of the present invention, a metallo-p-lactamase inhibitor comprising the compound represented by the general formula (I), or a salt thereof or a solvate thereof is provided. 5 [0032] The compound represented by the general formula (I) has a metallo- P-lactamase inhibitory effect, and the compound as such can be used as a metallo-P-lactamase inhibitor. [0033] 10 As described above, the metallo-1-lactamase hydrolyzes many P-lactam antibiotics and inactivates the effectiveness. Hence, the activities of the p-lactam antibiotics can be recovered by combining the compound represented by the general formula (I) with the antibiotics. 15 [0034] The compound represented by the general formula (I) can be used as such as the metallo-p-lactamase inhibitor, or can be preferably used as a pharmaceutical composition described below in a combination with carrier and furthermore with the 20 p-lactam antibiotics. [0035] The term "C 2

-

6 alkyl group" represented by R' may be either a linear or branched chain, preferably a C 2

.

4 alkyl group, such as an ethyl group, a n-propyl group, an i-propyl group, a n-butyl 25 group, an i-butyl group, a s-butyl group, a t-butyl group, a n-pentyl group, a neopentyl group, an i-pentyl group, a t-pentyl group, a n-hexyl group, an i-hexyl group, and the like, more preferably, an ethyl group, a n-propyl group, an i-propyl group, a n-butyl group, an i-butyl group, as-butyl group, and a t-butyl 30 group. The alkyl group may be substituted, and the substituent includes the "substituents" described above, and more preferably, a hydroxyl group, a thiol group, an amino group, and a halogen atom. [0036] 35 The term "C 1

.

6 alkyl group" represented by R 2 may be either a linear or branched chain, preferably a C 1

.

4 alkyl group, such as a methyl group, an ethyl group, a n-propyl group, an i-propyl group, a n-butyl group, an i-butyl group, a s-butyl group, a t-butyl group, a n-pentyl group, a neopentyl group, an i-pentyl group, a t-pentyl group, a n-hexyl group, an i-hexyl group, and 5 the like, more preferably, a methyl group, an ethyl group, a n-propyl group, an i-propyl group, a n-butyl group, an i-butyl group, a s-butyl group, and a t-butyl group. These alkyl groups may be substituted, and the substituent includes the "substituents" described above, and more preferably a thiol 10 group, an amino group, and a halogen atom. [0037] The term "C 3

.

7 cycloalkyl group" represented by R 1 or R2 preferably includes a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, a cycloheptyl group, and 15 the like, more preferably a cyclopentyl group, a cyclohexyl group, and a cycloheptyl group. The C 3

-

7 cycloalkyl group may be substituted, and the substituent includes the "substituent" described above, and more preferably a hydroxyl group, a thiol group, a C 1

.

6 alkyl group, an amino group, and a halogen atom. 20 In addition, the C 3

.

7 cycloalkyl group may be fused with the other rings such as aryl, preferably phenyl. [0038] The -C 1

.

3 alkylene-phenyl group represented by R1 or R 2 includes a benzyl group, a phenethyl group, a phenylpropyl 25 group, and the like. The phenyl group of the -C 1

.

3 alkylene-phenyl group may be substituted, and the substituent includes the "substituent" described above, and more preferably a hydroxyl group; a C 1

.

6 alkyl group; -COOM, wherein M represents a hydrogen atom or a pharmaceutically acceptable 30 cation; -CO-NR R , wherein R 2 and R , which may be the same or different, represents a hydrogen atom or a C 1

.

6 alkyl group, preferably a C 1

.

4 alkyl group, more preferably a C 1

-

2 alkyl group, wherein the alkyl group may be further substituted by an aminocarbonyl group, or R 2 and R , together with the nitrogen 35 atom to which R 22 and R 23 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl group, or a piperidyl group, and the heterocycle, especially the piperidyl group may be substituted by a hydroxyl group or a C 1

-

6 alkanoyloxy group; -O-R 2 4 wherein R 2 4 5 represents a C 1

-

6 alkyl group, preferably C 1

-

4 alkyl group, wherein the alkyl group may be substituted, and the substituent includes -COOM, wherein M represents a hydrogen atom, a C 1 .6 alkyl group or a pharmaceutically acceptable cation, an aminocarbonyl group, an amino group, a guanidino group, or a 10 five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, preferably, imidazole; a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms, preferably pyrrolidine; and a hydroxymethyl group. [0039] 15 The term "-Co- 1 alkylene-heterocycle" represented by R1 or R2 means -bond-heterocycle or -methylene-heterocycle, and the "heterocycle" has the same meaning as described above and preferably includes a five- to ten-membered mono- or bi-cyclic heterocycle having 1-4 hetero atoms selected from nitrogen, 20 oxygen, or sulfur atoms, more preferably a five- or six-membered saturated or unsaturated heterocycle having one nitrogen or oxygen atom. Specific examples of the "heterocycle" include tetrahydrofuran, furan, pyrrolidine, piperidine, pyrazolidine, imidazolidine, piperazine, morpholine, 25 thiomorpholine, pyrrole, thiophene, oxazole, isoxazole, thiazole, isothiazole, imidazole, pyrazole, pyridine, pyridazine, pyrimidine, pyrazine, triazole, tetrazole, thiadiazole, azetidine, thiazoline, quinuclidine, triazine, isobenzofuran, indole, indolizine, chromene, quinoline, isoquinoline, cinnoline, 30 quinazoline, quinoxaline, phthalazine, purine, pteridine, and the like. The bond or the methylene group may be bonded with any atoms on the heterocycle. One or more hydrogens on the heterocycle of the -Co- 1 alkylene-heterocycle may be substituted, and the substituent includes the "substituents" 35 described above, more preferably a hydroxyl group, a thiol group, a C 1

-

6 alkyl group, an amino group, and a halogen atom.

[0040] The term "-0-C 1

-

6 alkyl group" represented by R' or R 2 means a linear, branched or cyclic C 1

.

6 alkoxy group, preferably a -0-C 1

-

4 alkyl group including methoxy, ethoxy, propoxy, isopropoxy, 5 butoxy, isobutoxy, s-butoxy, t-butoxy, and the like, more preferably methoxy, ethoxy, propoxy, isopropoxy, t-butoxy, and the like. The portion of this alkyl group may be substituted, and the substituent includes the "substituents" described above, more preferably a hydroxyl group, a thiol group, a C 1

-

6 alkyl 10 group, an amino group, a halogen atom, and a phenyl group. [0041] The term "-S-C 1

-

6 alkyl group" represented by R 1 or R 2 means a linear, branched or cyclic C 1

-

6 alkylthio group, preferably a -S

-C

1

-

4 alkyl group including methylthio, ethylthio, propylthio, 15 isopropylthio, butylthio, isobutylthio, s-butylthio, t-butylthio, and the like, more preferably methylthio, ethylthio, propylthio, isopropylthio, t-butylthio, and the like. The portion of this alkyl group may be substituted, and the substituent includes the "substituents" described above, more preferably a hydroxyl 20 group, a thiol group, a C 1

-

6 alkyl group, an amino group, a halogen atom, and a phenyl group. [0042]

M

1 represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which may be 25 hydrolyzed in vivo. [0043] The term "pharmaceutically acceptable cation" represents a cation which may form a salt with one or both of the carboxyl groups of the general formula (I), and includes, for example, 30 alkali metals, alkaline earth metals, ammonium, organic bases, and the like, preferably lithium, sodium, potassium, magnesium, calcium, ammonium, ethanolamine, triethanolamine, trimethylamine, triethylamine, diisopropylamine, and the like. [0044] 35 The term "pharmaceutically acceptable group which may be hydrolyzed in vivo" represents an eliminable group which is bonded to one or both of the carboxyl groups of the general formula (I), and represents a group which may be metabolized in vivo, hydrolyzed and removed to form a carboxyl group. [0045] 5 "The pharmaceutically acceptable group which may be hydrolyzed in vivo" in the compound represented by the general formula (I) is preferably an ester moiety, and includes those moieties usually used, for example, a lower alkyl group, a lower alkenyl group, a lower alkylcarbonyloxy lower alkyl group, a 10 lower cycloalkylcarbonyloxy lower alkyl group, a lower cycloalkylmethylcarbonyloxy lower alkyl group, a lower alkenylcarbonyloxy lower alkyl group, an arylcarbonyloxy lower alkyl group, a tetrahydrofuranylcarbonyloxymethyl group, a lower alkoxy lower alkyl group, a lower alkoxy lower alkoxy 15 lower alkyl group, an arylmethyloxy lower alkyl group, an arylmethyloxy lower alkoxy lower alkyl group, a lower alkoxycarbonyloxy lower alkyl group, a lower alkoxycarbonyloxy lower alkoxy group, a lower cycloalkoxycarbonyloxy lower alkyl group, a lower cycloalkylmethoxycarbonyloxy lower alkyl group, 20 an aryloxycarbonyloxy lower alkyl group, a 3-phthalidyl group which may have substituents on the aromatic ring, a 2-(3-phthalidylidene)ethyl group which may have substituents on the aromatic ring, a 2-oxotetrahydrofuran-5-yl group, a mono-lower alkyl aminocarbonyloxymethyl group, a dilower 25 alkyl aminocarbonyloxymethyl group, a 2-oxo-5-lower alkyl-1,3-dioxolen-4-yl methyl group, a piperidinyl carbonyloxy lower alkyl group which may be substituted, a lower alkyl lower cycloalkylaminocarbonyloxy lower alkyl group, and the like. [0046] 30 "The pharmaceutically acceptable group which may be hydrolyzed in vivo" preferably includes a methyl group, an ethyl group, a 1-(cyclohexyloxycarbonyloxy)ethyl group, an acetoxymethyl group, a 1-(isopropyloxycarbonyloxy)ethyl group, a 1-(ethoxycarbonyloxy)ethyl group, a pivaloyloxymethyl 35 group, a cyclohexyloxycarbonyloxymethyl group, a 1-(isobutyloxycarbonyloxy)ethyl group, a 1-(cyclohexyloxycarbonyloxy)-2-methylpropan-1-y group, an isobutyloxycarbonyloxymethyl group, an isopropyloxycarbonyloxymethyl group, an isobutyryloxymethyl group, a (pentan-1-yl)oxycarbonyloxymethyl group, a 5 (butan-1-yl)oxycarbonyloxymethyl group, a (1 -ethylpropan-1-yl)oxycarbonyloxymethyl group, an isopentyloxycarbonyloxymethyl group, a (propan-1-yl)oxymethyl group, an ethoxycarbonyloxymethyl group, a neopentyloxycarbonyloxymethyl group, a 10 methoxycarbonyloxymethyl group, a cyclopentyloxycarbonyloxymethyl group, a t-butoxycarbonyloxymethyl group, a phthalidyl group, a 1-(methoxycarbonyloxy)ethyl group, a 1-(cyclopentyloxycarbonyloxy)ethyl group, a 15 (tetrahydropyran-4-yl)oxycarbonyloxymethyl group, a 1-(neopentyloxycarbonyloxy)ethyl group, a (piperidin-1-yl)carbonyloxymethyl group, an allyl group, a 1-(t-butoxycarbonyloxy)ethyl group, an (N,N-di-n-propylamino)carbonyloxymethyI group, a 20 phenyloxycarbonyloxymethyl group, a (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyl group, a (cis-2,6-dimethylpiperidin-1-yl)carbonyloxymethyl group, an N,N-di-(butan-1-yl)aminocarbonyloxymethyl group, a hexane-1-yl group, an 25 N-(hexane-1-y)-N-methylaminocarbonyloxymethyl group, an N,N-diisobutylaminocarbonyloxymethyl group, an N,N-diisopropylaminocarbonyloxymethyl group, an N-cyclohexyl-N-methylaminocarbonyloxymethyI group, an N-pentan-1-yl aminocarbonyloxymethyl group, an 30 N-cyclohexyl-N-ethylaminocarbonyloxymethyl group, an N-isobutyl-N-isopropylaminocarbonyloxymethyI group, an N-t-butyl-N-ethylaminocarbonyloxymethyl group, a 1-[(cis-2,6-dimethylpiperidin-1-yl)carbonyloxy]ethyl group, a 1-(N,N-diisopropylaminocarbonyloxy)ethyI group, 35 N-ethyl-N-isoamylaminocarbonyloxymethyl group, and the like. [0047] According to a preferred embodiment of the present invention, in the general formula (I), R' represents a C 2

-

6 alkyl group, a

C

3

-

7 cycloalkyl group, a hydroxymethyl group, a benzyl group, a phenethyl group, a -Co- alkylene-heterocycle, wherein 5 heterocycle represents tetrahydropyran, pyridine, or piperidine, a -0-C 1

-

6 alkyl group, or a -S-C 1

-

6 alkyl group, all of which may be substituted, R 2 represents a C 1

-

6 alkyl group, a C 3

.

7 cycloalkyl group, a hydroxymethyl group, a benzyl group, a phenethyl group, a -Co.

1 alkylene-heterocycle, wherein heterocycle 10 represents tetrahydropyran, pyridine, or piperidine, a -0-C 1

-

6 alkyl group, or a -S-C 1

-

6 alkyl group, all of which may be substituted, M', which may be the same or different, represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which may be hydrolyzed in 15 vivo. [0048] In this embodiment, the "substituent" has the same meaning as described above, and more preferably includes a hydroxyl group, a C 1

-

6 alkyl group, a C 1

-

6 alkoxy group, a carboxy group, 20 an amide group, a phenyl group, which may be fused with the ring, a heterocyclic carbonyl group which may be substituted, e.g. a piperidinecarbonyl group, an acyloxypiperidinecarbonyl group, a hydroxypiperidinecarbonyl group, a piperazinecarbonyl group, a morpholinecarbonyl group, and the like, a 25 heterocycle-C1-s alkoxy group such as an imidazolyl C 1

.

6 alkoxy group, a heterocycloxy group such as a pyrrolidineoxy group, an aminoxo C 1

-

6 alkylcarbamoyl group, a carboxyl group, an amino

C

1

-

6 alkoxy group, a guanidyl group, a guanidyl C 1

-

6 alkyl group, a guanidyl C 1

.

6 alkoxy group, a C 1

-

6 alkoxyoxo C 1

.

6 alkoxy 30 group, and the like. [0049]

M

1 may be the same or different, and represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which may be hydrolyzed in 35 vivo. [0050] "The pharmaceutically acceptable cation" preferably includes lithium, sodium, potassium, magnesium, calcium, ammonium, ethanolamine, triethanolamine, trimethylamine, triethylamine, diisopropylamine, and the like. 5 [0051] "The pharmaceutically acceptable group which may be hydrolyzed in vivo" represents an eliminable group which is bonded to one or both of the carboxyl groups of the general formula (I), and represents a group which may be metabolized 10 in vivo, hydrolyzed and removed to form a carboxyl group. [0052] "The pharmaceutically acceptable group which may be hydrolyzed in vivo" in the compound represented by the general formula (I) is preferably a methyl group, an ethyl group, a 15 1-(cyclohexyloxycarbonyloxy)ethyl group, an acetoxymethyl group, a 1-(isopropyloxycarbonyloxy)ethyl group, a 1-(ethoxycarbonyloxy)ethyl group, a pivaloyloxymethyl group, a cyclohexyloxycarbonyloxymethyl group, a 1-(isobutyloxycarbonyloxy)ethyl group, a 20 1-(cyclohexyloxycarbonyloxy)-2-methylpropan-1-yl group, an isobutyloxycarbonyloxymethyl group, an isopropyloxycarbonyloxymethyl group, an isobutyryloxymethyl group, a (pentan-1-yl)oxycarbonyloxymethyl group, a (butan-1-yl)oxycarbonyloxymethyl group, a 25 (1-ethylpropan-1-yl)oxycarbonyloxymethyl group, an isopentyloxyca rbonyloxymethyl group, a (propan-1-yl)oxymethyl group, an ethoxycarbonyloxymethyl group, a neopentyloxycarbonyloxymethyl group, a methoxycarbonyloxymethyl group, a 30 cyclopentyloxycarbonyloxymethyl group, a t-butoxycarbonyloxymethyl group, a phthalidyl group, a 1-(methoxycarbonyloxy)ethyl group, a 1-(cyclopentyloxycarbonyloxy)ethyl group, a (tetrahydropyran-4-yl)oxycarbonyloxymethyl group, a 35 1-(neopentyloxycarbonyloxy)ethyl group, a (piperidin-1-y)carbonyloxymethyl group, an allyl group, a 1-(t-butoxycarbonyloxy)ethyl group, an (N,N-di-n-propylamino)carbonyloxymethyl group, a phenyloxycarbonyloxymethyl group, a (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyl group, a 5 (cis-2,6-dimethylpiperidin-1-yl)carbonyloxymethyl group, an N,N-di-(butan-1-yl)aminocarbonyloxymethyl group, an hexane-1-yl group, an N-(hexane-1-y)-N-methylaminocarbonyloxymethyl group, an N,N-diisobutylaminocarbonyloxymethyl group, an 10 N,N-diisopropylaminocarbonyloxymethyl group, an N-cyclohexyl-N-methylaminocarbonyloxymethyl group, an N-pentan-1-yl aminocarbonyloxymethyl group, an N-cyclohexyl-N-ethylaminocarbonyloxymethyl group, an N-isobutyl-N-isopropylaminocarbonyloxymethyl group, an 15 N-t-butyl-N-ethylaminocarbonyloxymethyl group, a 1-[(cis-2,6-dimethylpiperidin-1-yl)carbonyloxy]ethyl group, a 1-(N,N-diisopropylaminocarbonyloxy)ethyl group, an N-ethyl-N-isoamylaminocarbonyloxymethyl group, and the like. [0053] 20 The preferred group of the compounds of the present invention includes the group of the compounds, in which R' represents a C 2

-

6 alkyl group; a C 3

.

7 cycloalkyl group, which may be substituted by a hydroxyl 25 group and may be fused with aryl, preferably phenyl; a hydroxymethyl group; a -C 1

.

3 alkylene-phenyl group, in which the phenyl group may be substituted and the substituent includes a hydroxyl group, a C 1

.

6 alkyl group, a hydroxymethyl group, -COOM, wherein M 30 represents a hydrogen atom or a pharmaceutically acceptable cation, -CO-NR R , wherein R 2 and R , which may be the same or different, represent a hydrogen atom or a C1.

6 alkyl group, preferably a C 1

-

4 alkyl group, more preferably a C 1

-

2 alkyl group, wherein the alkyl group may be further substituted by an 22 2 35 aminocarbonyl group, or R and R , together with the nitrogen atom to which R 22 and R 23 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl group, or a piperidyl group, and the heterocycle, especially the piperidyl group may be substituted by a hydroxyl 5 group or a C 1 .6 alkanoyloxy group, a group -O-R 2 4 wherein R 24 represents a C 1

.

6 alkyl group, preferably a C1- 4 alkyl group, wherein the alkyl group may be substituted, and the substituent includes -COOM, wherein M represents a hydrogen atom, a C1- 6 alkyl group or a pharmaceutically acceptable cation, an 10 aminocarbonyl group, an amino group, a guanidino group, or a five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, preferably, imidazole, a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms, preferably pyrrolidine; 15 a -Co- 1 alkylene-heterocycle, in which the heterocycle represents five- or six-membered saturated or unsaturated heterocycle comprising one nitrogen or oxygen atom, and may be substituted by a hydroxyl group; a -0-C 1

.

6 alkyl group; or 20 a -S-C 1

-

6 alkyl group, and

R

2 represents a C1.6 alkyl group; a C 3

-

7 cycloalkyl group, in which the ring may be substituted by a hydroxyl group and fused with aryl, preferably phenyl; 25 a hydroxymethyl group; a -C 1

-

3 alkylene-phenyl group, in which the phenyl group may be substituted and the substituent includes a hydroxyl group, a

C

1

.

6 alkyl group, a hydroxymethyl group, -COOM, wherein M represents a hydrogen atom or a pharmaceutically acceptable 30 cation, -CO-NR R , wherein R 2 and R , which may be the same or different, represent a hydrogen atom or a C 1

.

6 alkyl group, preferably a C 1

-

4 alkyl group, more preferably a C 1

-

2 alkyl group, wherein the alkyl group may be further substituted by an aminocarbonyl group, or R 2 and R , together with the nitrogen 35 atom to which R 22 and R 23 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl group, or a piperidyl group, and the heterocycle, especially the piperidyl group may be substituted by a hydroxyl group or a C 1

.

6 alkanoyloxy group, a group -O-R 2 4 wherein R 2 4 5 represents a C 1

.

6 alkyl group, preferably a C 1

.

4 alkyl group, wherein the alkyl group may be substituted, and the substituent includes -COOM, wherein M represents a hydrogen atom, a C 1

.

6 alkyl group or a pharmaceutically acceptable cation, an aminocarbonyl group, an amino group, a guanidino group, or a 10 five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, preferably, imidazole, a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms, preferably pyrrolidine; a -Co- 1 alkylene-heterocycle, in which the heterocycle represents 15 five- or six-membered saturated or unsaturated heterocycle comprising one nitrogen or oxygen atom, and may be substituted by a hydroxyl group; a -0-C 1

.

6 alkyl group; or a -S-C 1

.

6 alkyl group. 20 [0054] The more preferred group of the compounds of the formula (I) includes the group of the compounds, in which

R

1 represents a C 2

-

6 alkyl group, 25 a C 3

.

7 cycloalkyl group, which may be substituted by a hydroxyl group and may be fused with aryl, preferably phenyl; a hydroxymethyl group; a -C 1

.

3 alkylene-phenyl group, in which the phenyl group may be substituted by a hydroxyl group, a C 1

.

6 alkyl group, a 30 hydroxymethyl group, -COOM, wherein M represents a hydrogen atom or a pharmaceutically acceptable cation, -CO-NR R 2 , wherein R 22 and R 2 3 , which may be the same or different, represent a hydrogen atom or a C 1

.

6 alkyl group, preferably a

C

1

.

4 alkyl group, more preferably a C 1

-

2 alkyl group, wherein the 35 alkyl group may be further substituted by an aminocarbonyl group, or R 2 and R , together with the nitrogen atom to which

R

22 and R 23 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl group, or a piperidyl group, and the heterocycle, especially the piperidyl 5 group may be substituted by a hydroxyl group or a C1- 6 alkanoyloxy group, a group -O-R 24 wherein R 24 represents a C1- 6 alkyl group, preferably a C 1

.

4 alkyl group, wherein the alkyl group may be substituted, and the substituent includes -COOM, wherein M represents a hydrogen atom, a C 1

-

6 alkyl group or a 10 pharmaceutically acceptable cation, an aminocarbonyl group, an amino group, a guanidino group, or a five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, preferably, imidazole, a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms, preferably pyrrolidine; 15 a -Co.

1 alkylene-heterocycle, in which the heterocycle represents five- or six-membered saturated or unsaturated heterocycle comprising one nitrogen or oxygen atom, and may be substituted by a hydroxyl group; a -0-C 1

.

6 alkyl group; or 20 a -S-C 1

-

6 alkyl group, and

R

2 represents a C 1

.

6 alkyl group; a C 3

.

7 cycloalkyl group, in which the ring may be substituted by a hydroxyl group and fused with aryl, preferably phenyl; or 25 a -Ci- 2 alkylene-phenyl group. [0055] The more preferred group of the compounds includes the group of the compounds, in which R' represents 30 a C 2

-

6 alkyl group; a C 3

.

7 cycloalkyl group, in which the ring may be substituted by a hydroxyl group and fused with a phenyl group; a hydroxymethyl group; a C 1

-

2 alkylene-phenyl group, in which the phenyl group may be 35 substituted by a hydroxyl group, a C 1

.

6 alkyl group, a hydroxymethyl group, -COOM, wherein M represents a hydrogen atom or a pharmaceutically acceptable cation, -CO-NR 2

R

2 , wherein R 22 and R 23 , which may be the same or different, represent a hydrogen atom or a C 1

.

6 alkyl group, preferably a

C

1

.

4 alkyl group, more preferably a C 1

-

2 alkyl group, wherein the 5 alkyl group may be further substituted by an aminocarbonyl group, or R 2 and R , together with the nitrogen atom to which

R

22 and R 23 are bonded, form a five- or six-membered saturated heterocycle comprising 1-2 oxygen atoms or nitrogen atoms, preferably a morphonyl group, a piperazyl group, or a piperidyl 10 group, wherein the heterocycle, especially a piperidyl group may be substituted by a hydroxyl group or a C 1

-

6 alkanoyloxy group, a group -O-R 2 4 , wherein R 24 may be substituted by a C 1 alkyl group, preferably a C 1

-

4 alkyl group, which may be substituted by such substituents as -COOM, wherein M 15 represents a hydrogen atom, a C 1

.

6 alkyl group or a pharmaceutically acceptable cation, an aminocarbonyl group, an amino group, a guanidino group, or a five- or six-membered unsaturated heterocycle comprising 1-2 nitrogen atoms, preferably imidazole, or a five- or six-membered saturated 20 heterocycle comprising 1-2 nitrogen atoms, preferably pyrrolidine; a -Co.

1 alkylene-heterocycle, in which the heterocycle represents pyridine, morpholine, piperidine, or tetrahydropyran, and may be substituted by a hydroxyl group; 25 a -0-C 1

.

6 alkyl group, or a -S-C 1

.

6 alkyl group,

R

2 represents a C 1

-

6 alkyl group, or a C 3

.

7 cycloalkyl group. 30 [0056] The more preferred group of the compounds includes the group of the compounds, in which R1 represents a C 2

.

4 alkyl group; or 35 a C 3

-

7 cycloalkyl group, which may be substituted by a hydroxyl group, and fused with a phenyl group;

R

2 represents a C 1

.

4 alkyl group; or a C 3

.

7 cycloalkyl group. [0057] 5 The preferred examples of R 1 or R 2 preferably include an ethyl group, a n-propyl group, an i-propyl group, a cyclopentyl group, a 2,3-dihydro-1H-inden-2-yl group, a cyclohexyl group, a 2-(trans-4-hydroxycyclohexyl) group, a 2-(cis-4-hydroxycyclohexyl) group, a 2-(tetrahydropyran-4-yl) 10 group, a 2-[(piperidin-1-yl)methyl] group, a (pyridine-3-yl)methyl group, a (4-hydroxypiperidin-1-yl)methyl, a hydroxymethyl group, a (2-methylphenyl)methyl group, a methoxy group, a methylthio group, an isopropylthio group, a benzyl group, a 2-(4-hydroxybenzyl) group, a 4-methoxybenzyl 15 group, a 4-carboxybenzyl group, a 4-carbamoylbenzyl group, a 4-(4-acetoxypiperidin-1-carbonyl)benzyI group, a 4-(4-hydroxypiperidin-1-carbonyl)benzyl group, a 4-(2-amino-2-oxoethylcarbamoyl)benzyI group, a 4-hydroxybenzyl group, a 4-oxidobenzyl group (sodium salt), a 20 4-(carboxylatomethoxy)benzyl group, a 4-(2-methoxy-2-oxoethoxy)benzyl group, a 4-(2-amino-2-oxoethoxy)benzyl group, a 4-(2-amino-2-oxoethoxy)benzyl group, a 4-(2-aminoethoxy)benzyl group, a 25 4-(morpholine-1-carbonyl)benzyl group, a 4-(piperazine-1-carbonyl)benzyl group, a 4-[2-(1H-imidazol-1-yl)ethoxy]benzyI group, a 4-(2-guanidinoethoxy)benzyl group, a 4-(pyrrolidine-3-yloxy)benzyl group, a phenethyl group, and the 30 like, provided that R 1 does not represent a methyl group. [0058] In the general formula (I), the more preferred group of the compounds includes the group of the compounds, in which R' represents an ethyl group, a n-propyl group, an i-propyl 35 group, a cyclopentyl group, a 2,3-dihydro-1H-inden-2-yl group, a cyclohexyl group, a 2-(trans-4-hydroxycyclohexyl) group, a 2-(cis-4-hydroxycyclohexyl) group, a 2-(tetrahydropyran-4-yl) group, a 2-[(piperidin-1-yl)methyl] group, a (4-hydroxypiperidin-1-yl)methyl group, a hydroxymethyl group, a methoxy group, a methylthio group, an isopropylthio group, a 5 benzyl group, a 2-(4-hydroxybenzyl) group, a 4-carboxybenzyl group, a 4-carbamoylbenzyl group, a 4-(4-acetoxypiperidin-1-carbonyl)benzyl group, a 4-(4-hydroxypiperidin-1-carbonyl)benzyl group, a 4-(2-amino-2-oxoethylcarbamoyl)benzyl group, a 10 4-hydroxybenzyl group, a 4-oxidobenzyl group (sodium salt), a 4-(carboxylatomethoxy)benzyl group, a 4-(2-amino-2-oxoethoxy)benzyl group, a 4-(2-amino-2-oxoethoxy)benzyl group, a 4-(2-aminoethoxy)benzyl group, a 15 4-[2-(1H-imidazol-1-yl)ethoxy]benzyI group, a 4-(2-guanidinoethoxy)benzyl group, a phenethyl group, or the like, R 2 represents a methyl group, an ethyl group, a n-propyl group, an i-propyl group, a cyclopentyl group, a benzyl group, a phenethyl group, a (2-methylphenyl)methyl group, a 20 (pyridine-3-yl)methyl group, a 4-(morpholine-1-carbonyl)benzyl group, a 4-(piperazine-1-carbonyl)benzyl group, a 4-methoxybenzyl group, a 4-(2-methoxy-2-oxoethoxy)benzyl group, a 4-(pyrrolidine-3-yloxy)benzyl group, or the like. [0059] 25 Furthermore, specific examples of the compounds represented by the general formula (I) include: 2-ethyl-3-methylmaleic acid dimethyl ester, 2-ethyl-3-methylmaleic acid disodium 2,3-diethylmaleic acid diethyl ester, 30 2,3-diethylmaleic acid disodium, 2,3-diethylmaleic acid dipotassium, 2,3-di-n-propylmaleic acid diethyl ester, 2,3-di-n-propylmaleic acid disodium, 2-benzyl-3-methylmaleic acid dimethyl ester, 35 2-benzyl-3-methylmaleic acid disodium, 2-benzyl-3-ethylmaleic acid disodium, 3-ethyl-2-(4-hydroxybenzyl)maleic acid disodium, 2,3-dibenzylmaleic acid disodium, 2-benzyl-3-phenethylmaleic acid disodium, 2,3-diphenethylmaleic acid diethyl ester, 5 2,3-diphenethylmaleic acid disodium, 2-isopropyl-3-methylmaleic acid disodium, 3-ethyl-2-isopropylmaleic acid disodium, 2,3-diisopropylmaleic acid disodium, 3-benzyl-2-isopropylmaleic acid disodium, 10 2-isopropyl-3-(2-methylphenyl)methylmaleic acid disodium, 2-cyclopentyl-3-ethylmaleic acid disodium, 2-cyclopentyl-3-isopropylmaleic acid disodium, 3-benzyl-2-cyclopentylmaleic acid disodium, 2,3-dicyclopentylmaleic acid disodium, 15 2-(2,3-dihydro-1H-inden-2-yI)-3-isopropylmaleic acid disodium, 2-cyclohexyl-3-isopropylmaleic acid disodium, 2-(trans-4-hydroxycyclohexyl)-3-isopropylmaleic acid disodium, 2-(cis-4-hydroxycyclohexyl)-3-isopropylmaleic acid disodium, 3-isopropyl-2-(tetrahydropyran-4-yl)maleic acid disodium, 20 2-isopropyl-3-[(pyridine-3-yl)methyl]maleic acid disodium, 3-methyl-2-[(piperidin-1-yl)methyl]maleic acid dimethyl ester, 3-methyl-2-[(piperidin-1-yl)methyl]maleic acid disodium, 2-[(4-hydroxypiperidin-1-yl)methyl]-3-methylmaleic acid dimethyl ester, 25 2-[(4-hydroxypiperidin-1-yl)methyl]-3-methylmaleic acid disodium, 2-hydroxymethyl-3-methylmaleic acid disodium, 3-ethyl-2-methoxymaleic acid disodium, 3-ethyl-2-methylthiomaleic acid disodium 30 3-ethyl-2-isopropylthiomaleic acid disodium, 2-(4-carboxybenzyl)-3-isopropylmaleic acid dimethyl ester, 2-(4-carboxybenzyl)-3-isopropylmaleic acid trisodium, 2-(4-carbamoylbenzyl)-3-isopropylmaleic acid dimethyl ester, 2-(4-carbamoylbenzyl)-3-isopropylmaleic acid disodium, 35 2-isopropyl-3-[4-(morpholine-1-carbonyl)benzyl]maleic acid dimethyl ester, 2-isopropyl-3-[4-(morpholine-1-carbonyl)benzyl]maleic acid disodium, 2-isopropyl-3-[4-(piperazine-1-carbonyl)benzyl]maeic acid dimethyl ester hydrochloride, 5 2-isopropyl-3-[4-(piperazine-1-carbonyl)benzyl]maleic acid disodium, 2-[4-(4-acetoxypiperidin-1-carbonyl)benzyl]-3-isopropylmaleic acid dimethyl ester, 2-[4-(4-hydroxypiperidin-1-carbonyl)benzyl]-3-isopropylmaleic 10 acid disodium, 2-[4-(2-amino-2-oxoethylcarbamoyl)benzyl]-3-isopropylmaleic acid dimethyl ester, 2-[4-(2-amino-2-oxoethycarbamoyl)benzyl]-3-isopropylmaleic acid disodium, 15 2-isopropyl-3-(4-methoxybenzyl)maleic acid disodium, 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester, 2-(4-oxidobenzyl)-3-isopropylmaleic acid trisodium, 2-isopropyl-3-[4-(2-methoxy-2-oxoethoxy)benzyl]maleic acid dimethyl ester, 20 2-[4-(carboxylatomethoxy)benzyl]-3-isopropylmaleic acid trisodium, 2-[4-(2-amino-2-oxoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester, 2-[4-(2-amino-2-oxoethoxy)benzyl]-3-isopropylmaleic acid 25 disodium, 2-[4-(2-aminoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester, 2-[4-(2-aminoethoxy)benzyl]-3-isopropylmaleic acid disodium, 2-{4-[2-(1H-imidazol-1-yl)ethoxy]benzyl}-3-isopropylmaleic 30 acid dimethyl ester, 2-{4-[2-(1H-imidazol-1-yl)ethoxy]benzyl}-3-isopropylmaleic acid disodium, 2-isopropyl-3-[4-(pyrrolidine-3-yloxy)benzyl]maleic acid dimethyl ester, 35 2-isopropyl-3-[4-(pyrrolidine-3-yloxy)benzyl]maleic acid disodium, 2-[4-(2-guanidinoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester hydrochloride, and 2-[4-(2-guanidinoethoxy)benzyl]-3-isopropylmaleic acid disodium. 5 [0060] The compound of the general formula (I) is preferably a pharmaceutically acceptable salt, which includes an acid addition salt. Thus, the compound of the general formula (I) can be used in the form of a salt derived from mineral acids or 10 organic salts, which include an acetic acid salt, an adipic acid salt, an alginic acid salt, an aspartic acid salt, a benzoic acid salt, a benzenesulfonic acid salt, a hydrogensulfate salt, a butyric acid salt, a citric acid salt, a camphoric acid salt, a camphorsulfonic acid salt, a cyclopentanpropionic acid salt, a 15 digluconic acid salt, a dodecylsulfuric acid salt, an ethanesulfonic acid salt, a fumaric acid salt, a glucoheptanoic acid salt, a glycerophosphoric acid salt, a hemisulfuric acid salt, a heptanoic acid salt, a hexanoic acid salt, a hydrochloride salt, a hydrobromic acid salt, a hydroiodic acid salt, a 20 2-hydroxyethanesulfonic acid salt, a lactic acid salt, a maleic acid salt, a methanesulfonic acid salt, a 2-naphthalenesulfonic acid salt, a nicotinic acid salt, an oxalic acid salt, a pamoic acid salt, a pectic acid salt, a persulfuric acid salt, a 3-phenylpropionic acid salt, a picric acid salt, a pivalic acid salt, 25 a propionic acid salt, a succinic acid salt, a tartaric acid salt, a thiocyanic acid salt, a tosylic acid salt, and an undecanoic acid salt. [0061] The compound of the general formula (I) may be in the form of 30 a solvate. [0062] The solvent of the solvate includes water, methanol, ethanol, isopropanol, butanol, acetone, ethyl acetate, chloroform, and the like. 35 [0063] The compound of the general formula (I) or a salt thereof may contain asymmetric carbons in the molecule, and each of the isomers or all of the mixtures of the isomers are included in the present invention. Also, the compound of the general formula (I) may be used in the form of a prodrug. The prodrugs can be 5 hydrolyzed in vivo and thus preferably used for oral administration because of its good absorption from gastric mucosa or intestinal mucosa, resistance against acid, and the other factors. [0064] 10 Pharmaceutical uses and pharmaceutical compositions The compound of the general formula (I) has, as described above, metallo-p-lactamase inhibitory effect and thus used for the inhibition of metallo-p-lactamase. As one of the specific embodiments of its applications, the compound of the present 15 invention can be used in combination with antibiotics which will be inactivated by the action of metallo- P-lactamase, among others p-lactam antibiotics to recover the activities of these antibiotics for the therapy of infections. [0065] 20 Therefore, according to one embodiment of the present invention, a metallo-P-lactamase inhibitor and pharmaceutical composition comprising the compound of the general formula (I) as an effective ingredient which is used in combination with p-lactam antibiotics is provided. That is, the 25 metallo- P-lactamase inhibitor and pharmaceutical composition according to the present invention is administered concomitantly or sequentially with P-lactam antibiotics. [0066] The P-lactam antibiotics include carbapenems, penicillins, 30 cephems, or prodrugs thereof. [0067] Specific examples of the carbapenems include imipenem, meropenem, biapenem, doripenem, ertapenem, tebipenem (pivaloyloxymethyl(4R,5S,6S)-6-[(R)-1-hydroxyethyl]-4-methyl 35 -7-oxo-3-{[1-(1,3-thiazoline-2-yl)azetidine-3-yl]thio})-1-azabic yclo[3.2.0]hept-2-ene-2-carboxylate), CS-023 ((-)-(4R, 5S,6S) -3- [[(3S, 5S) -5- [(S) -3- (2-g u a ni di noacetylam ino) pyrrolidin- 1-ylcarbonyl]-1 -methylpyrrolid in-3-yl]thio]-6-[(R)- 1 hydroxyethyl] -4-methyl-7-oxo-aza bicyclo[3.2.0] hept-2-ene-2-c arboxylic acid) and ME1036 5 ((1S,5R,6S)-2-[7-(1-carbamoylmethylpyridinium-3-yl)carbonyli midazo[5, 1 -b]thiazol-2-yl]-6-((1 R)- 1-hydroxyethyl)-1-methyl- 1 -carbapen-2-em-3-carboxylate), and the like. Particularly, the carbapenem which is preferably used in combination with the compound of the general formula (I) is imipenem, meropenem, 10 biapenem and doripenem. [0068] Examples of the penicillins include benzylpenicillin, phenoxymethylpenicillin, carbenicillin, azidocillin, propicillin, ampicillin, amoxicillin, epicillin, ticarcillin, ciclacillin, pirbenicillin, 15 azlocillin, mezlocillin, sulbenicillin, piperacillin, and the other well known penicillins. These penicillins can be used in the form of their prodrugs, for example, as in vivo hydrolyzable esters such .as acetoxymethyl, pivaloyloxymethyl, 1-(ethoxycarbonyloxy)ethyl and phthalidyl esters of ampicillin, 20 benzylpenicillins and amoxicillin, as aldehyde or ketone addition products of penicillins having a 6-a-aminoacetamide side chain including, for example, similar derivatives of hetacillin, metampicillin and amoxicillin, and further as esters such as phenyl or indanyl esters of penicillins having a 25 6-a-carboxyacetamide side chain such as carbenicillin and ticarcillin. Particularly preferred penicillins used in combination with the compound of the general formula (I) ampicillin, amoxicillin, carbenicillin, piperacillin, azlocillin, mezlocillin and ticarcillin. These penicillins can be used in the form of a 30 pharmaceutically acceptable salt thereof such as a sodium salt. As another form, ampicillin or amoxicillin can be used in the form of a suspension for injection or of amphoteric granules for a suspension for injection (ampicillin trihydrate or amoxicillin trihydrate) in combination with the compound of the general 35 formula (I). [0069] Cephems include, for example, cefatrizine, cefaloridine, cefalotin, cefazolin, cephalexin, cephacetrile, cefapilin, cefamandole naphate, cefradine, 4-hydroxycephalexin, cefoperazone, latamoxef, cefminox, flomoxef, cefsulodin, 5 ceftadizime, cefuroxime, cefditoren, cefmetazole, cefotaxime, ceftriaxone, cefepime, cefpirome, cefozopran, as well as the other well know cephems, and these cephems can also be used in the form of their prodrugs. Particularly preferred cephems used in combination with the compound of the general formula 10 (I) are cefotaxime, ceftriaxone, ceftadizime and cefepime, which can be used in the form of a pharmaceutically acceptable salt such as a sodium salt. [0070] According to the preferred embodiment of the present 15 invention, it is also preferred to combine the compound of the general formula (I) and carbapenem antibiotics in the presence of a dehydropeptidase (DHP) inhibitor, because many carbapenems are liable to be hydrolyzed by DHP. The preferred DHP inhibitor includes cilastatin or a salt thereof. 20 [0071] According to the preferred embodiment of the present invention, it is preferred to combine further the other serine-p-lactamase inhibitor in addition to the compound of the general formula (I), and the preferred examples of the other 25 serine-p-lactamase inhibitor include clavulanic acid, sulbactam or tazobactam. [0072] The metallo-p-lactamase producing strain which is preferably used in combination with the antibiotics and the compound of 30 the general formula (I) includes, for example, Bacillus cereus, Bacteroides fragilis, Escherichia coli, Aeromonas hydrophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, Stenotrophomonas maltophilia, Shigella flexneri, Alcaligenes xylosoxidans, Legionella gormanii, 35 Chryseobacterium meningosepticum, Chryseobacterium indologenes, Acinetobacter baumannii, Citrobacter freundiiand Enterobacter cloacae, and the like. [0073] The doses of the compound of the general formula (I) and the antibiotic can be varied over a wide range, for example, 5 generally in a proportion of 1:0.5-20 by weight, preferably 1 1-8. [0074] The compound of the general formula (I) and the 1-lactam antibiotic may also be administered individually or in the form of 10 a unit composition containing the both effective ingredients. In any of the embodiments, the compound of the general formula (I) and/or the antibiotic are preferably combined with a pharmaceutically acceptable carrier, i.e. an additive for a preparation, to form a pharmaceutical composition. 15 [0075] The pharmaceutical composition according to the present invention may be administered orally or parenterally. It is possible to envisage parenteral administration routes including intranasal, intraocular, intra-aural, transdermal, intratracheal, 20 intrarectal, intraurinary, subcutaneous, intramuscular and intravenous routes. Examples of the preparation suitable for oral administration include, for example, tablets, particles, granules, powders, syrups, solutions, capsules, chewables, or suspensions. Examples of the preparation suitable for parenteral 25 administration include, for example, injections, droplets, inhalants, nebulae, suppositories, vaginal suppositories, percutaneous absorbent, transmucosal absorbent, eye drops, ear drops, nose drops, or patches. Liquid preparations such as injections or drops may be provided, for example, as a 30 pharmaceutical composition in the form of lyophilized powder, which may be dissolved or suspended into water or the other appropriate solvents such as physiological saline, or glucose infusion at the time of use. [0076] 35 Carriers, i.e. additives for preparations can be appropriately selected according as the forms of the pharmaceutical composition, and include, but are not limited to, stabilizers, surfactants, plasticizers, lubricants, solubilizing agents, buffering agents, sweetenings, bases, adsorbents, flavoring agents, binding agents, suspending agents, brightening agents, 5 coating agents, aromatizing agents, perfumes, humectants, humidity modifiers, fillers, anti-foaming agents, masticatories, refrigerants, colorants, sugar-coating agents, isotonicity agents, pH modifiers, softeners, emulsifiers, adhesives, adhesion intensifiers, viscous agents, thickeners, foaming agents, 10 excipients, dispersants, propellants, disintegrating agents, disintegration accelerators, fragrances, anti-humectants, aseptics, preservatives, analgesics, solvents, liquefacients, dissolution accelerators, fluidizing agents, and the like, and two or more of these additives may be combined. Specific examples 15 of these additives for preparations are described, for example, in "The Dictionary of Pharmaceutical Additives" (Ed. by Japan Pharmaceutical Excipients Council, Yakuji Nippo Limited), a person skilled in the art can select an appropriate additive for preparation according to the form of a pharmaceutical 20 composition to prepare the desired form of the pharmaceutical composition according to the process widely used in the field. Generally, the pharmaceutical composition can be prepared so that the substance as the effective ingredient is in the range of 1.0-100% (w/w), preferably 1.0-60% (w/w). 25 [0077] Specific examples of the carrier preferably include gelatin, lactose, refined sugar, titanium oxide, starch, crystalline cellulose, hyd roxypro pylmethylcellulose, carboxymethylcellulose, cornstarch, microcrystalline wax, white 30 petrolatum, magnesium aluminometasilicate, anhydrous calcium phosphate, citric acid, trisodium citrate, hydroxypropyl cellulose, sorbitol, sorbitan esters of fatty acid, polyisobate, sucrose esters of fatty acid, polyoxyethylene hydrogenated castor oil, polyvinylpyrrolidone, magnesium stearate, light 35 anhydrous silicic acid, talc, vegetable oil, benzyl alcohol, gum arabic, propylene glycol, polyalkylene glycol, cyclodextrin, and hydroxypropylcyclodextrin. However, the additives for pharmaceutical preparations are not limited to these examples. [0078] Doses and administration frequencies of the medicaments of the 5 present invention are not particularly limited, and suitable doses and administration frequencies can be determined depending on various factors such as purpose of administration, type of a disease, the age, body weight and symptoms of a patient and the like. In the case of oral administration, the medicament can 10 be administered once to several times a day in an amount of 1 100 mg/kg based on the weight of the compound of the general formula (I) as a daily dose for an adult. In the case of parenteral administration, the medicament may preferably be administered once to several times a day in an amount of 0.1 15 100 mg/kg based on the weight of the compound of the general formula (I) as a daily dose for an adult. [0079] Also, according to another embodiment of the present invention, a therapeutic process of infection comprising administering 20 concomitantly or sequentially the compound of the general formula (I), a P-lactam antibiotic, and optionally furthermore a P-lactamase inhibitor or dehydropeptidase (DHP) inhibitor to animals including human being is provided. [0080] 25 Furthermore, according to another embodiment of the present invention, the use of the compound of the general formula (I) in order to prepare a pharmaceutical composition, especially a therapeutic agent of infections comprising a p-lactam antibiotic, and optionally furthermore a p-lactamase inhibitor or 30 dehydropeptidase (DHP) inhibitor is provided. [0081] Preparation of the compound The compound of the general formula (I) according to the present invention can be preferably prepared by the methods 35 shown in the Schemes A-H or the similar methods. [0082] As herein used, Me represents a methyl group, Et represents an ethyl group, TBS represents a t-butyldimethylsilyl group, TBDPS represents a t-butyldiphenylsilyl group, Ac represents an acetyl group, and Bn represents a benzyl group. 5 [0083] Scheme A

R

1

R

2

R

1

R

2

P

1 0 OP M 1 0 OM 1 0 0 00 Al (I-a) Scheme B RI R 2 RI R 2 0 - M 1 0- C M 1 000 00 5(- a) 10 Scheme C 1) R 1 or 2 Mgxl CuBr-Me 2 S RI R 2

R

1

R

2 2) RI ir 2X P p'0 2 C C0 2

P

1

P

1 04 OP' - M'O M 1 00 00 C2 (I-a) Scheme D R' R 2 R' R 2

R

1

R

2

R

1

R

2 HO OH M 1 0 m 00 0 0 00 _ m 3(I-a) Scheme E Rior 2 RI R 2 R R 2

OP

1

P

1 0 OP' M'0 OMl 0 0 0 00 15 E2 (I-a) Scheme F R' or 2 R'or 2 RI Or 2 R1 or 2 R1 o 2R er 2 OK _ HO ___ HO __

P

1 O ,'pl P 1 0 OP 1 HO OH 0 0 0 0 0 0 El E El EA R1 R 2

R

1

R

2 O' = O MO0 OM E0 0 0 F5I-a) Scheme F' R1o2 R1 o2 R 1 ' O 2

R

1 a2 R 1 ' 2

R

1 o 2 _____ HO_ ___ HO _____

P

1 < O0O OPl HO OH O 0 00 00 El E2 E3 EA

R

1

R

2

R

1

R

2 R" R 2

R

1

R

2 0 ~Q 00 00 0 0 MeO OMe MeO OMe M'O OM' E5 ( - a) Scheme G R' R 1 2 X2 R 1 2 R" R 1 2 R" R"H P1O OP 1 P'O OP, P'O OP M'O OM' 0 0 00 00 0 0 1 3 (1 - b) 5 Scheme H Rior X H R'o 2 OH R' - OH - H 2 0 O O Ot: O" M O O O M I 0 0o 0 0 0 al (I-c) [0084] In the aforementioned Schemes A-H, R1, R 2 , and M 1 have the 10 same meanings as those in R 1 , R 2 , and M 1 of the general formula (I). The compounds (I-a), (I-b) and (I-c) represent the compounds of the general formula (I) wherein M 1 represents a pharmaceutically acceptable cation. Also, R" represents a hydroxyl group, a thiol group, a -0-C 1

-

6 alkyl group, a -S-C 1

.

6 15 alkyl group, an amino group, an N-mono-substituted amino group, an N,N-di-substituted amino group, a heterocycle containing one nitrogen atom. X1 represents a halogen atom, P1 and P 2 represent a protective groups of a carboxyl group. [0085] 20 In Schemes A-H, it is possible to carry out at need the step of eliminating a protective group, in the case that the desired substituent is not conform to the reaction condition of synthesis used, with first introducing the substituent in the form of a protected derivative and eliminating the protective group after 25 the reaction. It is also possible to convert the substituent itself via the step of eliminating a protective group at need.

[0086] The protective group may be appropriately determined by referring to Protective Groups in Organic Synthesis (T. W. Greene et al., Wiley, New York (1999)), and the like. 5 [0087] In the present invention, the protective group required is mainly a hydroxyl protective group, an amino protective group, or a carboxyl protective group. [0088] 10 Examples of the hydroxyl protective group include an acetyl group, a pivaloyl group, silyl groups such as triethylsilyl group, t-butyldimethylsilyl (TBS) group, and t-butyldiphenylsilyl (TBDPS) group, a benzyl group, a trytyl group, an o-nitrobenzyloxycarbonyl group, a p-nitrobenzyloxycarbonyl 15 group, a benzyloxycarbonyl group, an allyloxycarbonyl group, a t-butyloxycarbonyl group, a 2,2,2-trichloroethyloxycarbonyl group, and the like. [0089] Examples of the amino protective group include an acetyl group, 20 a t-butoxycarbonyl group, a benzyl group, a benzyloxycarbonyl group, a benzenesulfonyl group, an o -nitrobenzenesulfonyl group, p -nitrobenzenesulfonyl group, and the like. [0090] Examples of the carboxyl protective group include a methyl 25 group, an ethyl group, a t-butyl group, an allyl group, a benzhydryl group, a 2-naphthylmethyl group, a benzyl group, a silyl group such as t-butyldimethylsilyl (TBS) group, a phenacyl group, a p-methoxybenzyl group, an o-nitrobenzyl group, a p -methoxyphenyl group, a p-nitrobenzyl group, a 4-pyridylmethyl 30 group, a 1-(cyclohexyloxycarbonyloxy)ethyl group, an acetoxymethyl group, a 1-(isopropyloxycarbonyloxy)ethyl group, a 1-(ethoxycarbonyloxy)ethyl group, a pivaloyloxymethyl group, a cyclohexyloxycarbonyloxymethyl group, and the like. [0091] 35 Scheme A This scheme illustrates a preferred process in case that a maleate diester, the raw material Al, is easily commercially available from a commercial source or can be easily prepared by a variety of methods well known in the art. [0092] 5 The conversion of the compound Al to the compound (I-a) is carried out by changing M1 into a metal cation at need, reacting the compound Al with two or more equivalents of an aqueous solution of alkali hydroxide such as sodium hydroxide, potassium hydroxide, and lithium hydroxide in tetrahydrofuran, 10 dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C-90 *C for 5 min to 48 h, then concentrating the reaction mixture at reduced pressure, and drying the concentrate in vacuum. Thus, the compound (I-a) can be 15 obtained. [0093] Scheme B This scheme illustrates a preferred process in case that a maleic anhydride, the raw material B1, is easily commercially available 20 from a commercial source or can be easily prepared by a variety of methods well known in the art. [0094] The compound (I-a) is obtained by the ring opening reaction of the acid anhydride, the compound B1, according to the 25 conventional method. If necessary, after M' is changed into a metal cation, the compound B1 is reacted with two or more equivalents of an aqueous solution of alkali hydroxide such as sodium hydroxide, potassium hydroxide, and lithium hydroxide in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, 30 dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 OC-90 OC for 5 min to 48 h, then the reaction mixture is concentrated at reduced pressure, and the concentrate is dried in vacuum to give the compound (I-a). [0095] 35 Scheme C The conversion from the compound C1 to the compound C2 in this scheme can be carried out on the basis of the known method described in the literature (E. S. Ratemi et al., J. Org. Chem. 1996, 61, 6296) according to the following steps. [0096] 5 An acetylene dicarboxylic acid ester, the raw material C1, is easily commercially available from a commercial source or can be easily prepared by a variety of methods well known in the art. [0097] 10 The compound C1 is reacted with one equivalent or an excessive amount of magnesium alkyl halide R' (or R 2)MgX, in the presence of one equivalent or an excessive amount of bromo(dimethyl sulfide)copper(I) in tetrahydrofuran, dioxane, diethyl ether, toluene, benzene, hexamethylphosphoric 15 triamide, dimethylformamide, dichloromethane, dichloroethane, hexane, or a mixed solvent thereof at -80 *C to 0 *C for 5 min to 5 h. Then, the anionic product at 2-position obtained is reacted with hexamethylphosphoric triamide and alkyl halide R' (or R 2 )X1 at -80 *C to 50 *C for 5 min to 5 h to give the 20 compound C2 after usual treatment. [0098] R1 (or R 2 )MgXl includes, for example, benzylmagnesium bromide, benzylmagnesium chloride, n-propylmagnesium chloride, t-butylmagnesium chloride, s-butylmagnesium 25 bromide, c-hexylmagnesium bromide, c-pentylmagnesium bromide, ethylmagnesium bromide, ethylmagnesium chloride, n-heptylmagnesium bromide, n-hexylmagnesium bromide, i-propylmagnesium bromide, i-propylmagnesium chloride, methylmagnesium bromide, methylmagnesium iodide, 30 n-octylmagnesium bromide, n-pentadecylmagnesium bromide, n-pentylmagnesium bromide, phenylmagnesium bromide, phenylmagnesium chloride, phenylmagnesium iodide, n-propylmagnesium bromide, n-tetradecylmagnesium chloride, o-trylmagnesium bromide, m-trylmagnesium bromide, p 35 -trylmagnesium bromide, and vinylmagnesium bromide.

[0099] Also, R1 (or R 2 )Xl includes, for example, methyl iodide, ethyl iodide, n-propyl iodide, i-propyl iodide, n-butyl iodide, s-butyl iodide, i-butyl iodide, methyl bromide, ethyl bromide, n-propyl 5 bromide, i-propyl bromide, n-butyl bromide, s-butyl bromide, and i-butyl bromide. [0100] Next, the conversion of the compound C2 into the compound (I-a) can be carried out according to the following steps. In this 10 case, after M1 is changed into a metal cation, the compound C2 is reacted with two equivalents or more of an aqueous solution of alkali hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, 15 methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C to 90 *C for 5 min to 48 h, then the reaction mixture is concentrated at reduced pressure, and the concentrate is dried in vacuum to give the compound (I-a). [0101] 20 Scheme D This scheme illustrates a preferred process in case that a succinic acid derivative, the raw material D1, is the one which is easily commercially available from a commercial source or can be easily prepared by a variety of methods well known in the art 25 (e.g. Japanese Patent Publication No. 2003-513890). The conversion of the compound D1 into the compound D3 can be carried out on the basis of the known method described in the literature (M. J. Kates et al., J. Org. Chem. 1996, 61, 4164). That is, the compound D1 is reacted with one equivalent or an 30 excessive amount of a halogenated formic acid ester such as methyl chloroformate or ethyl chloroformate in the presence of one equivalent or an excessive amount of a base in tetrahydrofuran, dioxane, diethyl ether, toluene, benzene, hexamethylphosphoric triamide, dimethylformamide, 35 dichloromethane, dichloroethane, hexane, or a mixed solvent thereof at -80 IC to 80 *C for 5 min to 8 h, and the reaction mixture is concentrated under reduced pressure to give the compound D2. The aforementioned base includes, for example, organic bases such as diisopropylethylamine, triethylamine, 2,6-lutidine, pyridine, N-methylmorpholine, and the like, and 5 inorganic bases such as sodium hydride, sodium hydroxide, potassium hydroxide, sodium methoxide, sodium ethoxide, potassium t-butoxide, sodium hydrogen carbonate, potassium hydrogen carbonate, sodium carbonate, potassium carbonate, cesium carbonate, and the like. 10 [0102] Next, the conversion of the compound D2 into the compound D3 can be carried out by the following method. That is, the compound D2is reacted with one equivalent or an excessive amount of trimethylsilyl trifluoromethanesulfonic acid in the 15 presence of a base in tetrahydrofuran, dioxane, diethyl ether, toluene, benzene, hexamethylphosphoric triamide, dimethylformamide, dichloromethane, dichloroethane, hexane, or a mixed solvent thereof at -80 oC to 100 OC for 5 min to 5 h, then with a catalytic amount or more of tetrabutylammonium 20 bromide and one equivalent or an excessive amount of bromine at -80 *C to 100 OC for 5 min to 8 h, and then the reaction mixture is treated in the usual manner to give the compound D3. The aforementioned base includes, for example, organic bases such as diisopropylethylamine, triethylamine, 2,6-lutidine, 25 pyridine, N-methylmorpholine, and the like, and inorganic bases such as sodium hydride, sodium hydroxide, potassium hydroxide, sodium methoxide, sodium ethoxide, potassium-t-butoxide, sodium hydrogen carbonate, potassium hydrogen carbonate, sodium carbonate, potassium carbonate, 30 cesium carbonate, and the like. [0103] Then, the compound (I-a) is obtained by the ring opening reaction of the compound D3, the acid anhydride, according to the conventional method. If necessary, after M1 is changed into 35 a metal cation, the compound D3 is reacted with two equivalents or more of an aqueous solution of an alkali hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C 5 to 90 *C for 5 min to 48 h, and the reaction mixture is concentrated under reduced pressure and dried in vacuum to give the compound (I-a). [0104] Scheme E 10 The conversion of the compound El to the compound E2 can be carried out by the following method on the basis of the known method described in the literature (H. Hagiwara et al., Synthetic Commun. 1984, 14, 1193). The a-halogenated carboxylic acid derivative El is commercially available from a commercial 15 source or can be easily prepared by a variety of methods well known in the art. The compound El is reacted with one equivalent or an excessive amount of a strong base such as lithium diisopropylamide, lithium hexamethyldisilazane, potassium hexamethyldisilazane, sodium hydride, potassium 20 hydride, or the like and a catalytic amount or more of a copper (I) halide such as copper (I) chloride, copper (I) bromide, and copper (I) iodide in tetrahydrofuran, dioxane, diethyl ether, toluene, benzene, hexamethylphosphoric triamide, dimethylformamide, dichloromethane, dichloroethane, hexane, 25 or a mixed solvent thereof at -90 *C to 20 *C for 5 min to 5 h, and then the reaction mixture is treated in the usual manner to give the compound E2. In addition, this scheme is based on the dimer type reaction and thus most appropriate to the synthesis of symmetrically 2,3-di-substituted maleic acids. 30 [0105] Next, the conversion of the compound E2 to the compound (I-a) can be carried out by the following method. After M 1 is changed into a metal cation, the compound E2 is reacted with two equivalents or more of an aqueous solution of an alkali 35 hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C to 90 *C for 5 min to 48 h, then the reaction mixture is concentrated at reduced pressure and dried in vacuum to give 5 the compound (I-a). [0106] Scheme F The conversion of the compounds F1 and F2 to the compound F5 can be carried out by the following method on the basis of 10 the known method described in the literature (S. B. Singh et al., Bioorg. Med. Chem. 2000, 8, 571). The pyruvic acid derivative F1 and the compound F2 are commercially available from a commercial source or can be easily prepared by a variety of methods well known in the art. That is, one equivalent or an 15 excessive amount of compound F2 is reacted with one equivalent or an excessive amount of a strong base such as lithium diisopropylamide, lithium hexamethyldisilazane, potassium hexamethyldisilazane, sodium hydride, or potassium hydride in tetrahydrofuran, dioxane, diethyl ether, toluene, 20 benzene, hexamethylphosphoric triamide, dimethylformamide, dichloromethane, dichloroethane, hexane, or a mixed solvent thereof at -90 *C to 20 *C for 5 min to 5 h. The enolate thus obtained is reacted with the compound F1 at -90 *C to 20 *C for 5 min to 5 h, the reaction mixture is treated in the usual 25 manner to give the compound F3. [0107] Next, the conversion of the compound F3 to the compound F4 can be carried out by the following method. The compound F3 is reacted with two equivalents or more of an aqueous solution of 30 an alkali hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water or a mixed solvent thereof at 70 *C to 100 *C for 1 h to 48 h, and then the reaction 35 mixture is treated in the usual manner to give the compound F4.

[0108] When P' in the compound F3 is a protective group such as benzyl group which can be removed by hydrogenation reaction, the conversion of F3 into the compound F4 can be carried out 5 by the following manner. That is, the compound F3 is hydrogenated under a vigorous stirring condition in the presence of a catalytic amount of a metal catalyst such as nickel or palladium in methanol, ethanol, tetrahydrofuran, dioxane, diethyl ether, hexane, toluene, water or a mixed solvent thereof 10 in the hydrogen stream at 00 C to 50 *C for 1 h to 48 h, and then the reaction mixture is treated in the usual manner to give the compound F4. [0109] Next, the conversion of the compound F4 to the compound F5 15 can be carried out by the following method. The compound F4 is reacted with an excessive amount of acetic anhydride at 70 *C to 140 *C for 1 h to 48 h, and then the reaction mixture is treated in the usual manner to give the compound F5. [0110] 20 The compound (I-a) is obtained by the ring opening reaction of the compound F5, the acid anhydride, to give the compound (I-a) according to the standard method. After M 1 is changed into a metal cation at need, the compound F4 is reacted with two equivalents or more of an aqueous solution of an alkali 25 hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like at 0 *C to 90 0 Cfor 5 min to 48 h in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof, and then the reaction mixture is 30 concentrated under reduced pressure and dried in vacuum to give the compound (I-a). [0111] Scheme F' The conversion from the compounds F1 and F2 into the 35 compound F5 can be carried out by the method described in Scheme F. R" and R 2 have the same meanings as those in R1, and either or both of R" or R 2 represent a different substituent from R' or R 2 [0112] Next, the conversion from the compound F5 into the compound 5 F6 can be carried out by the following method. The compound F5 is reacted with one equivalent or an excessive amount of trimethylsilyl diazomethane or the like in an alcohol such as methanol at 0 *C to 20 *C for 5 min to 8 h, and the reaction mixture is subjected to the usual post-treatment to give the 10 compound F6. [0113] The conversion of the compound F6 into the compound F7 can be carried out by a variety of methods known in the art, in which R' and R 2 in the compound F6 are changed into the other 15 R'and R 2 . [0114] Next, compound F7 OD compound (I-a) can be carried out by the following method. In this case, after M' is changed into a metal cation, the compound F7 is reacted with two equivalents or 20 more of an aqueous solution of an alkali hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C to 90 *C for 5 min to 48 h, and 25 then the reaction mixture is concentrated under reduced pressure and dried in vacuum to give the compound (I-a). [0115] Scheme G The conversion from the compound G1 into the compound G2 30 can be carried out by the following method. That is, the compound G1 which is easily commercially available from a commercial source or can be easily prepared by a variety of methods known in the art (e.g., E. S. Ratemi et al., .. Org. Chem. 1996, 61, 6296) is reacted with one equivalent or an 35 excessive amount of N-halogenated succinimide such as N-bromosuccinimide or N-chlorosuccinimide and a catalytic amount or more of a radical initiator such as 2,2'-azobisisobutyronitrile, dibenzoyl peroxide, and the like in carbon tetrachloride at 0 *C to 80 *C for 5 min to 48 h, and the reaction mixture is subjected to the usual post-treatment to 5 give the compound G2. [0116] Next, the conversion of the compound G2 into compound G3 can be carried out by the following method. That is, the compound G2 is reacted with water, hydrogen sulfide, alcohol, 10 thiol or the like in tetrahydrofuran, dioxane, diethyl ether, toluene, benzene, hexamethylphosphoric triamide, dimethylformamide, dichloromethane, dichloroethane, hexane or a mixed solvent thereof in the presence of a primary amine, a secondary amine including a cyclic amine or a base at -80 *C 15 to 100 *C for 5 min to 48 h, and the reaction mixture is subjected to the usual post-treatment to give the compound G3. [0117] Next, the conversion of the compound G3 into the compound (I-b) can be carried out by the following method. In this case, 20 after M' is changed into a metal cation, the compound G3 is reacted with two equivalents or more of an aqueous solution of an alkali hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, 25 methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C to 90 *C for 5 min to 48 h, and the reaction mixture is then concentrated under reduced pressure and dried in vacuum to give the compound (I-b). [0118] 30 Scheme H The compound H1 in this scheme is easily commercially available from commercial sources or can be easily manufactured by a variety of methods well known in the art (e.g., A. M. Despande et al., Synthesis 2001, 5, 702). The 35 conversion from the compound H1 into the compound H2 can be carried out by the following method. That is, the compound Hi is reacted with an excessive amount of water in the presence of a base in tetrahydrofuran, dioxane, diethyl ether, hexamethylphosphoric triamide, dimethylformamide or a mixed solvent thereof at 0 *C to 100 *C for 5 min to 48 h, and the 5 reaction mixture is then subjected to the usual post-treatment to give the compound H2. [0119] Next, the conversion of the compound H2 into the compound (I-c) can be carried out by the following method. In this case, 10 after M 1 is changed into a metal cation, the compound H2 is reacted with two equivalents or more of an aqueous solution of an alkali hydroxide such as sodium hydroxide, potassium hydroxide, lithium hydroxide, and the like in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, 15 methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 *C to 90 *Cfor 5 min to 48 h, and the reaction mixture is then concentrated under reduced pressure and dried in vacuum to give the compound (I-c). [0120] 20 As described above, the compounds (I-a), (I-b) and (I-c) obtained in Schemes A-H can be refined by chromatography with non-ionic porous resin, gel filtration with Sephadex, normal phase or reversed phase chromatography, crystallization, and the other methods, if necessary. 25 [0121] Conversion of M' in the general formula (I) The compound (I-a), (I-b) or (I-c) as the alkali salt such as a sodium salt or a potassium salt is reacted with two equivalents or more of a hydrochloride salt of amines such as ammonia, 30 methylamine, dimethylamine, trimethylamine, ethylamine, diethylamine, or triethylamine in tetrahydrofuran, dioxane, diethyl ether, acetonitrile, dimethylformamide, methanol, ethanol, n-propanol, water, or a mixed solvent thereof at 0 0 C to 90 0 C for 5 min to 48 h, and the reaction mixture is then 35 concentrated under reduced pressure and dried in vacuum to give the ammonium salt of the general formula (I).

[0122] The ammonium salt of the general formula (I) thus obtained can also be refined by chromatography with non-ionic porous resin, gel filtration with Sephadex, normal phase or reversed phase 5 chromatography, crystallization, and the other methods, if necessary. [0123] Also, the compound of the general formula (I) in which M 1 is an in vivo hydrolyzable group can be obtained by the following 10 method. [0124] The compound described above is obtained by reacting the above described (I-a), (I-b) or (I-c) in the form of an alkali metal salt such as a sodium salt or a potassium salt in which M 1 15 is a metal cation with a halide compound of the in vivo hydrolyzable group M 6

-X

2 . [0125]

M

6 has the same meaning as the in vivo hydrolyzable group of the general formula (I) described above, X 2 represents an 20 eliminable group such as chlorine, bromine, iodine, -OSO 2

CF

3 ,

-OSO

2

CH

3 , -OSO 2 PhCH 3 , and the like. The compound of the general formula (I) can be obtained by reacting (I-a), (I-b) or (I-c), as occasion demands, with two equivalents or more of an alkyl halide (M 6

-X

2 :) in the presence of a catalytic amount or an 25 excessive amount of a base at -70 *C to 50 *C, preferably at -30 *C to 30 *C for 10 min to 24 h, thus obtaining the compound of the general formula (I) in which M1 is an in vivo hydrolyzable group. The base described above includes organic bases such as diisopropylethylamine, 30 1,8-diazabicyclo[5.4.0]-7-undecene, 2,6-lutidine, and the like and inorganic bases such as sodium hydroxide, potassium hydroxide, sodium hydrogen carbonate, potassium hydrogen carbonate, sodium carbonate, potassium carbonate, cesium carbonate, and the like. The alkyl halide described above is 35 represented by M 6

-X

2 , in which X 2 represents a halogen atom or an eliminable group and includes preferably iodine, bromine, or chlorine. Examples include methyl iodide, ethyl iodide, 1-(cyclohexyloxycarbonyloxy)ethyl iodide, acetic acid bromomethyl 1-(isopropyloxycarbonyloxy)ethyl iodide, 1-(ethoxycarbonyloxy)ethyl iodide, iodomethyl pivalate, 5 cyclohexyloxycarbonyloxymethyl iodide, 1 -(isobutyloxycarbonyloxy)ethyl iodide, 1-(cyclohexyloxycarbonyloxy)-2-methylpropan-1-yl iodide, isobutyloxycarbonyloxymethyl iodide, isopropyloxycarbonyloxymethyl iodide, isobutyryloxymethyl 10 iodide, (pentan-1-yl)oxycarbonyloxymethyl iodide, (butan-1-yl)oxycarbonyloxymethyl iodide, (1-ethylpropan-1-yl)oxycarbonyloxymethyl iodide, isopentyloxycarbonyloxymethyl iodide, (propan-1-yl)oxymethyl iodide, ethoxycarbonyloxymethyl iodide, 15 neopentyloxycarbonyloxymethyl iodide, methoxycarbonyloxymethyl iodide, cyclo pentyloxyca rbonyloxymethyl iodide, t-butoxycarbonyloxymethyl iodide, 3-bromophthalide, 1 -(methoxycarbonyloxy)ethyl iodide, 20 1-(cyclopentyloxycarbonyloxy)ethyl iodide, (tetrahydropyran-4-yl)oxycarbonyloxymethyl iodide, 1-(neopentyloxycarbonyloxy)ethyl iodide, (piperidin-1-yl)carbonyloxymethyl iodide, allyl iodide, 1- (t- butoxyca rbonyloxy)ethyl iodide, 25 N,N-di(propan-1-yl)aminocarbonyloxymethyl iodide, phenyloxycarbonyloxymethyl iodide, (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyl bromide, (Z)-2-(3-phthalidylidene)ethyl bromide, (cis-2,6-dimethylpiperidin-1-yl)carbonyloxymethyl chloride, 30 chloromethyl N,N-di-n-butylcarbamate, 1-iodohexane, chloromethyl N-n-hexyl-N-methylcarbamate, chloromethyl N,N-diisobutylcarbamate, chloromethyl N,N-diisopropylcarbamate, chloromethyl N-cyclohexyl-N-methylcarbamate, chloromethyl 35 N-pentan-1-ylcarbamate, chloromethyl N-cyclohexyl-N-ethylcarbamate, chloromethyl N-isobutyl-N-isopropylcarbamate, chloromethyl N-t-butyl-N -ethylcarbamate, 1-chloroethyl N,N-diisopropylcarbamate, 1-[(cis-2,6-dimethylpiperidin-1-yl)carbonyloxy]ethyl chloride, 5 chloromethyl N-ethyl-N-isoamylcarbamate, and the like. In a single or mixed inert solvent (e.g., N,N-dimethylformamide, N,N-dimethylacetamide, N,N-diethylformamide, N,N-diethylacetamide, N-methylpyrrolidinone, N,N-dimethylimidazolidinone, dimethylsulfoxide, sulfolane, 10 acetonitrile, acetone, ethyl acetate, tetrahydrofuran, 1,4-dioxane, diethyl ether, anisole, dichloromethane, 1,2-dichloroethane, chloroform, toluene, benzene, hexamethylphosphoric triamide, methanol, ethanol, and the like. It is also possible to obtain the compound of the general 15 formula (I) by preliminarily introducing an in vivo hydrolyzable group into the corresponding portion to M 1 at the initial stage of Schemes A-H and conducting the treatment according to each of the schemes. [0126] 20 The ester compound (I) thus obtained can be isolated and refined by precipitation, gel filtration with Sephadex, normal phase or reversed phase chromatography, and the like. [0127] Novel compounds represented by the general formulae (II), 25 (III), (IV) and (V) The group of the compounds represented by the general formula (I) includes novel compounds. Thus, according to another embodiment of the present invention are provided novel 2,3-di-substituted maleic acid derivatives, specifically the novel 30 compounds represented by the general formulae (II), (III), (IV) and (V). [0128] Compound of the general formula (II) In the formula (II), R 3 represents a C 2

.

6 alkyl group, a C 3

.

7 35 cycloalkyl group or a hydroxymethyl group, all of which may be substituted, and R 4 represents a C 1

.

6 alkyl group or a C 3

.

7 cycloalkyl group, all of which may be substituted. As herein used, the terms "C 1

.

6 alkyl group" or "C 2 . alkyl group" represented by R 3 or R4 may be either of a linear or branched chain and preferably represent a C 1

.

4 alkyl or a C 2

-

4 alkyl group, 5 including, for example,a methyl group, an ethyl group, a n-propyl group, an i-propyl group, a n-butyl group, an i-butyl group, a s-butyl group, a t-butyl group, a n-pentyl group, a neopentyl group, an i-pentyl group, a t-pentyl group, a n-hexyl group, an i-hexyl group, and the like. The term represented by 10 "C 3

-

7 cycloalkyl group" represented by R 3 or R 4 includes preferably a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, a cycloheptyl group, and the like, more preferably a cyclopentyl group, a cyclohexyl group, and a cycloheptyl group. 15 [0129] All of these groups may be substituted as described on the general formula (I), and their preferred examples are also the same as in the general formula (I). [0130] 20 M 2 represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which may be hydrolyzed in vivo. [0131] The term "pharmaceutically acceptable cation" represented by 25 M 2 has the same meaning as the general formula (I). [0132] Compound of the general formula (III)

R

5 represents an ethyl group, and R 6 represents a C 1

.

3 linear alkyl group, i.e. a methyl group, an ethyl group and a propyl 30 group. [0133] M3 may be the same or different and represents a "pharmaceutically acceptable cation", and a "pharmaceutically acceptable group which may be hydrolyzed in vivo". In other 35 words, it represents a salt or an ester which has an in vivo hydrolyzable group.

[0134] The term "pharmaceutically acceptable cation" represented by

M

3 has the same meanings as those of the general formulae (I) and (II). 5 [0135] The term "pharmaceutically acceptable group which may be hydrolyzed in vivo" represented by M 3 represents an eliminable group linked to one or both of the carboxyl groups of the general formula (III), which represents a group metabolized, 10 hydrolyzed and removed in vivo to give a carboxyl group, and has the same meanings as those of the general formula (II). [0136] Compound of the general formula (IV) The terms "C 1

-

6 alkyl group" represented by R 7 and "C 3

.

7 15 cycloalkyl group" represented by R 7 have the same meanings as those of the general formulae (I) and (II), and their preferred examples also have the same meanings as those of the general formula (I). [0137] 20 The term "-C 1

-

3 alkylene-phenyl group" represented by R 7 or R 8 has the same meanings as those of the general formula (I), and their preferred examples also have the same meanings as those of the general formula (I). [0138] 25 The terms "-C1 alkylene-ring A" represented by R 7 or "-Co.

1 alkylene-ring A" represented by R 8 represent that the alkylene is linked with the ring A via methylene or methylene/bond, and the ring A represents a five- to ten-membered mono-cyclic or bi-cyclic heterocyclic ring containing 1 to 4 nitrogen, oxygen or 30 sulfur atoms, more preferably, a five- or six-membered saturated or unsaturated heterocycle containing one nitrogen or oxygen atom. The specific examples of the "heterocycle" include tetrahydrofuran, furan, pyrrolidine, piperidine, pyrazolidine, imidazolidine, piperazine, morpholine, thiomorpholine, pyrrole, 35 thiophene, oxazole, isoxazole, thiazole, isothiazole, imidazole, pyrazole, pyridine, pyridazine, pyrimidine, pyrazine, triazole, tetrazole, thiadiazole, azetidine, thiazoline, quinuclidine, triazine, isobenzofuran, indole, indolizine, chromene, quinoline, isoquinoline, cinnoline, quinazoline, quinoxaline, phthalazine, purine, pteridine, and the like. The bond or the methylene group 5 may be linked with any position of the heterocycle. However, if

R

7 is a C 1

.

6 alkyl group, R 8 does not represent dihydrofuran. [0139] The terms "-0-C 1

.

6 alkyl group" represented by R 7 or R 8 and

"-S-C.

6 alkyl group" represented by R 7 or R 8 have the same 10 meanings as those of the general formula (I), and their preferred examples also have the same meanings as those of the general formula (I). [0140] These groups may be substituted as described above. 15 M 4 , which may be the same or different, represents a hydrogen atom, a "pharmaceutically acceptable cation" or a "pharmaceutically acceptable group which may be hydrolyzed in vivo". [0141] 20 The term "pharmaceutically acceptable cation" represented by

M

4 has the same meanings as those of the general formulae (I) and (II), and the preferred examples also have the same meanings as those of the general formula (I). [0142] 25 The term "pharmaceutically acceptable group which may be hydrolyzed in vivo" represented by M 4 represents an eliminable group which is linked with one or both of the carboxyl groups of the general formula (IV), which represents a group metabolized, hydrolyzed and removed in vivo to give a carboxyl group, and 30 has the same meanings as those of the general formula (II). [0143] Compound of the general formula (V) The terms "C 1 s 6 alkyl group" represented by R 9 and "C 3

.

7 cycloalkyl group" represented by R 9 have the same meanings as 35 those of the general formula (I) and (II), and the preferred examples also have the same meanings as those of the general formula (I). [0144] The term "-C 1

.

3 alkylene-phenyl group" represented by R' or R" has the same meanings as those of the general formula (I), and 5 the preferred examples also have the same meanings as those of the general formula (I). [0145] The term "-Co- 1 alkylene-ring B" represented by R 9 or R1 represents that the alkylene is linked with the ring B via a bond 10 or methylene, and the ring B represents pyridine, piperidine, or tetrahydropyran. However, R 9 and Rlo do not represent pyridinium which is a quaternary salt formed by bonding at the nitrogen atom on the pyridine ring. [0146] 15 The terms "-0-C 1

.

6 alkyl group" or "-S-C 1

-

6 alkyl group" represented by R 9 or R1 0 have the same meanings as those of the general formula (I), and the preferred examples also have the same meanings as those of the general formula (I). [0147] 20 These groups may be substituted as described above. The substituent Ms, which may be the same or different, represents a hydrogen atom, a "pharmaceutically acceptable cation" or a "pharmaceutically acceptable group which may be hydrolyzed in vivo". 25 [0148] The term pharmaceuticallyy acceptable cation" represented by

M

4 has the same meanings as those of the general formulae (I) and (II), and the preferred examples also have the same meanings as those of the general formula (I). 30 [0149] The term "pharmaceutically acceptable group which may be hydrolyzed in vivo" represented by M 4 represents an eliminable group which is linked with one or both of the carboxyl groups of the general formula (V), which represents a group metabolized, 35 hydrolyzed and removed in vivo to give a carboxyl group, and has the same meanings as those of the general formula (II).

[0150] The compounds of the general formulae (II)-(V) can be prepared according to the above described methods for preparing the compound of the general formula (I). 5 [0151] Also, the compounds of the general formulae (II)-(V) can be also prepared in the similar manner to the compound of the general formula (I) by well known methods, or the methods described in Schemes A-H or similar thereto. 10 [Examples] [0152] The present invention is now explained by way of examples below, but it is not limited to these examples. In addition, the structures of the compounds prepared in Examples are shown in 15 Tables 1-1 - 5. [0153] Example 1: 2-ethyl-3-methylmaleic acid dimethyl ester To a suspension of bromo(dimethylsulfide)copper (I) (1.08 g, 20 5.25 mmol) in anhdrous tetrahydrofuran (24 mL), a 1.06 M ethylmagnesium chloride-tetrahydrofuran solution (4.8 mL, 5.1 mmol) was added dropwise under vigorous stirring at -50 *C. The mixture was stirred at the same temperature for 2 h, and further stirred at -78 *C for 5 min. Then, a solution of dimethyl 25 acetylenedicarboxylate (563 mg, 3.96 mmol) in tetrahydrofuran (8 mL) was added dropwise to the mixture. After 40 min, a solution of 4 mL of hexamethylphosphoric triamide in tetrahydrofuran (4 mL) was added dropwise, and a solution of methyl iodide (1.44 g, 10.1 mmol) in tetrahydrofuran (8 mL) 30 was further added dropwise. After stirring for 20 min, the reaction mixture was warmed up to room temperature. An aqueous solution of saturated ammonium chloride (10 mL, adjusted to pH 8 with aqueous ammonia) was added at -20 *C, and the temperature was again raised to room temperature. The 35 reaction solvent was evaporated under reduced pressure, and diethyl ether and water were added to separate the phases. The aqueous layer was extracted three times with diethyl ether, and the combined organic layer was washed with an aqueous solution of saturated ammonium chloridewater, and brine. The residue obtained by concentrating the organic layer under 5 reduced pressure was subjected to silica gel column chromatography (hexane - ethyl acetate 9 : 1) to give 98.2 mg of the title compound as a syrup (yield: 14%). 'H NMR (CDCl 3 ) 6 1.07 (t, J = 7.5 Hz, 3H), 1.95 (s, 3H), 2.37 (q, J = 7.5 Hz, 2H), 3.75 (s, 3H), 3.78 (s, 3H). 10 [0154] Example 2: 2-Ethyl-3-methylmaleic acid disodium 2-Ethyl-3-methylmaleic acid dimethyl ester (86.5 mg, 0.465 mmol) was dissolved in 1,4-dioxane (1.0 mL), 1 M sodium 15 hydroxide aqueous solution (0.93 mL, 0.93 mmol) was added at room temperature, and the mixture was stirred at 50 *C for 4 h. The reaction solvent was evaporated under reduced pressure, and the residue thus obtained was dried in vacuum to give 93.8 mg (quantitative) of the title compound as a solid. 20 'H NMR (D 2 0) 6 0.81 (t, J = 7.6 Hz, 3H), 1.64 (s, 3H), 2.08 (q, J = 7.6 Hz, 2H); MS (FAB+) m/z 203 [(M+H)+]. [0155] Example 3: 2,3-Diethyl maleic acid diethyl ester 25 To a solution of diisopropylamine (654 mg, 6.46 mmol) in anhydrous tetrahydrofuran (5 mL) under the atmosphere of argon was added a 1.58 M n-butyl lithium-hexane solution (3.9 mL, 6.4 mmol) with stirring under ice cooling, and the mixture was stirred at 0 OC for 10 min and at -90 *C for 5 min. To the 30 mixture, a solution of ethyl 2-bromo-n-butyrate (1.00 g, 5.13 mmol) in anhydrous tetrahydrofuran (9.5 mL) was added dropwise at -90 *C, and the mixture was stirred for 30 min. Copper (I) iodide (489 mg, 2.56 mmol) was added at the same temperature, and the mixture was vigorously stirred further 5 35 min. The temperature was raised to 0 *C with stirring, and a saturated aqueous ammonium chloride solution (30 mL) was added. The reaction mixture was directly filtered, and the filtrate was concentrated under reduced pressure. The residue was diluted with water and extracted twice with ethyl acetate, and the combined organic layer was washed sequentially with 5 water and brine and concentrated. The residue thus obtained was subjected to silica gel column chromatography (hexane ethyl acetate 9 1) to give 368.4 mg of the title compound as oil (yield: 63%). 1H NMR (CDCl3) 5 1.07 (t,

-

= 7.5 Hz, 6H), 1.30 (tH z = 7.1 Hz, 10 6H), 2.37 (q, J= 7.5 Hz, 4H), 4.22 (q, 3 = 7.1 Hz, 4H); MS (ESI+) m/z 229 [(M+H)*]. [0156] 2,3-Diethylmaleic acid disodium 15 To a solution of 2,3-diethylmaleic acid diethyl ester (368 mg, 1.61 mmol) in 1,4-dioxane (3.2 mL was added a 1 M sodium hydroxide aqueous solution (3.2 mL, 3.2 mmo) at room temperature, and the mixture was stirred at 50 OC for 15 h. The reaction mixture was concentrated under reduced pressure, and 20 the residue obtained was dried in vacuum to give 346 mg of the title compound as a colorless solid (quantitative). iH NMR (D20) 5 0.83 (t, J = 7.5 Hz, 6H), 2.11 (q, 3 = 7.5 Hz, 4H); MS (FAB+) m/z 217' [(M+H)*]. [0157] 25 Example 5: 2,3-Diethylmaleic acid dipotassium 2 ,3-Diethylmaleic acid diethyl ester was reacted with 1 M aqueous potassium hydroxide solution In the same manner as Example 4 to give the title compound as a solid (quantitative). 30 MS (FAB+) m/z 249 [(M+H)*]. [0158] 2,3-Di-n-propylmaleic acid diethyl ester The title compound was obtained as oil (yield: 85/) from ethyl 35 2 -bromo-n-valerate in the same manner as Example 3. 1H NMR (CDCI3) 6 0.95 (t, 3 = 7.5 Hz, 6H), 1.30 (t, I = 7.2 Hz, 6H), 1.47 (m, 4H), 2.33 (m, 4H), 4.20 (q, J = 7.2 Hz, 4H). [0159] Example 7: 2,3-Di-n-propylmaleic acid disodium 5 The title compound was obtained as a solid (quantitative) from 2,3-di-n-propylmaleic acid diethyl ester in the same manner as Example 2. 'H NMR (D 2 0) 6 0.74 (t, J = 7.5 Hz, 6H), 1.20 (tq, J = 7.5, 7.8 Hz, 4H) 2.07 (t, J = 7.8 Hz, 4H); MS (FAB+) m/z 245 [(M+H)+]. 10 [0160] Example 8: 2-Benzyl-3-methylmaleic acid dimethyl ester Dimethyl acetylenedicarboxylate was reacted with benzylmagnesium chloride and methyl iodide in the same 15 manner as Example 1 to give the title compound as oil (yield: 24 %). 'H NMR (CDCl 3 ) 6 2.04 (s, 3H), 3.66 (s, 3H), 3.72 (s, 2H), 3.78 (s, 3H), 7.15-7.32 (m, 5H). [0161] 20 Example 9: 2-Benzyl-3-methylmaleic acid disodium The title compound was obtained as a solid (quantitative) from 2-benzyl-3-methylmaleic acid dimethyl ester in the same manner as Example 2. 25 'H NMR (D 2 0) 6 1.75 (s, 3H), 3.49 (s, 2H), 7.09-7.23 (m, 5H); MS (FAB+) m/z 265[(M+H)f]. [0162] Example 10 (Synthesis Example): 2-Benzyl-3-ethylmaleic anhydride 30 a) To anhydrous tetrahydrofuran (5 mL) was added diisopropylamine (759 mg, 7.5mmol) under nitrogen stream, and the solution was cooled to -78 *C. To this solution was added a 1.58 M n-butyl lithium-hexane solution (4.3 mL, 6.8 mmol), and the mixture was stirred at -78 *C for 5 min and at 35 0 OC for 15 min. A solution of lithium diisopropylamide tetrahydrofuran thus obtained above was cooled to -78 *C. To the solution, a solution of 3-phenylpropionic acid ethyl ester (891 mg, 5.0 mmol) in anhydrous tetrahydrofuran (10 mL) was added dropwise over 1 h, and the reaction mixture was further stirred at -78 *C for 1 h. The cold solution of the enolate thus 5 prepared above was added dropwise via a cannula to a solution of ethyl 2-ketobutyrate (781 mg, 6.0 mmol) in anhydrous tetrahydrofuran (5 mL) having been cooled to -78 *C. After stirring at -78 *C for 2 h, the reaction mixture was quenched with a 10% aqueous citric acid solution, and extracted with 10 ethyl acetate. The organic layer was washed with 1 M hydrochloric acid, a saturated aqueous sodium hydrogen carbonate solution and brine, dried over anhydrous magnesium sulfate, and filtered. The residue obtained by concentrating the filtrate under reduced pressure was subjected to silica gel 15 column chromatography (hexane - ethyl acetate 9 : 1) to give 1.12g of 3-benzyl-2-ethyl-2-hydroxysuccinic acid diethyl ester as oil in the form of a diastereomer mixture (yield: 7 3 %). [0163] b) The 3-Benzyl-2-ethyl-2-hydroxysuccinic acid diethyl ester 20 thus obtained was dissolved in 1,4-dioxane (5 mL), added with water (5 mL) and 5 M sodium hydroxide aqueous solution (15 mL), and stirred at 100 *C for 15 h. The reaction mixture was cooled to room temperature, adjusted to pH 1 with 1 M hydrochloric acid, and extracted with ethyl acetate. The organic 25 layer having been washed with 1 M hydrochloric acid and brine was dried over anhydrous magnesium sulfate and then filtered. The organic layer was concentrated under reduced pressure to give 1.0 g (quantitative) of 3-benzyl-2-ethyl-2-hydroxysuccinic acid as a solid. 30 [0164] c) The 3-Benzyl-2-ethyl-2-hydroxysuccinic acid obtained was dissolved in acetic anhydride (35 mL) and stirred at 110 *C for 15 h. The residue obtained by evaporating the acetic anhydride of the reaction mixture under reduced pressure was subjected 35 to silica gel column chromatography (hexane - toluene - ethyl acetate 8 : 3 : 1) to give 619 mg of the title compound as oil (yield: 79%). 'H NMR (CDCI 3 ) 6 1.13 (t, J = 7.8 Hz, 3H), 2.49 (q, J = 7.8 Hz, 2H), 3.79 (s, 2H), 7.18-7.49 (m, 5H); MS (EI) m/z 216 (M+). [0165] 5 Example 11: 2-benzyl-3-ethylmaleic acid disodium To a solution of 2-benzyl-3-ethylmaleic anhydride (619 mg, 2.86 mmol) in 1,4-dioxane (3.0 mL) was added 1 M aqueous sodium hydroxide solution (5.73 mL, 5.73 mmol) at room 10 temperature, and the mixture was stirred for 10 min. The reaction mixture was concentrated under reduced pressure, and the residue obtained was dried in vacuum to give 800 mg (quantitative) of the title compound as a colorless solid. 'H NMR (D 2 0) 6 0.85 (t, J = 7.5 Hz, 3H), 2.20 (q, J = 7.5 Hz, 15 2H), 3.79 (s, 2H), 7.08-7.31 (m, 5H); MS (FAB+) m/z 279 [(M + H)+] [0166] Example 12 (Synthesis Example): Ethyl 3-(4-t-butyldimethylsilyloxyphenyl)propionate 20 To a solution of ethyl 3-(4-hydroxyphenyl)propionate (940 mg, 4.84 mmol) in anhydrous dimethylformamide (9.4 mL) were added t-butyldimethylsilyl chloride (1.0 g, 6.6 mmol) and imidazole (490 mg, 1.5 mmol) at room temperature, and the mixture was left standing at the same temperature for 14 h. 25 The reaction mixture was directly concentrated, and subjected to silica gel column chromatography (hexane - ethyl acetate 25 : 1 - 20 : 1) to give 1.49 g (quantitative) of the title compound as a solid. 'H NMR (CDCl 3 ) 6 0.18 (s, 6H), 0.97 (s, 9H), 1.23 (t, J = 7.0 30 Hz, 3H), 2.57 (t, J = 7.3 Hz, 2H), 2.88 (t, J = 7.3 Hz, 2H), 4.12 (q, J = 7.0 Hz, 2H), 6.57 (m, 2H), 6.87 (m, 2H). [0167] Example 13 (Synthesis Example): 2-(4-Acetoxybenzyl)-3-ethylimaleic anhydride 35 a) Under the atmosphere of argon, to a solution of diisopropylamine (572 mg, 5.65 mmol) in anhydrous tetrahydrofuran (7 mL) was added a 1.54 M n-butyl lithium-hexane solution (3.5 mL, 5.4 mmol) with stirring under ice cooling, and the mixture was stirred at 0 *C for 10 min and then at -78 *C for 5 min.To the mixture, a solution of ethyl 5 3-(4-t-butyldimethylsilyloxyphenyl)propionate (1.47 g, 4.78 mmol) in anhydrous tetrahydrofuran (7 mL) was added dropwise at the same temperature over 6 min, and the reaction mixture was further stirred for 20 min. The cold solution of the enolate thus prepared above was added dropwise via a cannula 10 into a solution of ethyl 2-ketobutyrate (518 mg, 3.98 mmol) in anhydrous tetrahydrofuran (5 mL) having been cooled to -78 *C. After stirring at -78 *C for 1 h, the reaction mixture was quenched and adjusted to pH 4 with acetic acid, and the temperature was raised to room temperature. The reaction 15 solvent was evaporated under reduced pressure, and the residue was diluted with ethyl acetate and water to separate the phases. The organic layer having been washed with brine was concentrated under reduced pressure. The residue obtained was subjected to silica gel column chromatography (hexane - ethyl 20 acetate 10 1 -+ 5 1) to give diethyl 3-(4-t-butyldimethylsilyloxybenzyl)-2-ethyl-2-hydroxysuccinate as oil in amounts of 433 mg (low polar diastereomer, yield: 25%) and 290 mg (high polar diastereomer, yield: 17%), respectively. 25 [0168] b) To a solution of diethyl 3-(4-t-butyldimethylsilyloxybenzyl)-2-ethyl-2-hydroxysuccinate 425 mg (0.968 mmol) in 1,4-dioxane(3.9 mL) was added 1 M lithium hydroxide aqueous solution (3.9 mL, 3.9 mmol) at room 30 temperature, and the mixture was stirred at 60 *C for 22 h. The reaction mixture was adjusted to pH 1 with 1 M hydrochloric acid with stirring under ice cooling. The solvent was evaporated under reduced pressure, and the residue thus obtained was dissolved in ethyl acetate, and washed with water and and 35 brine. The organic layer was concentrated under reduced pressure and dried in vacuum to give 343 mg (crude) of 3-(4-t- butyldimethylsilyloxybenzyl) -2-ethyl-2-hyd roxysuccin ic acid as a solid. [0169] c) To the crude 5 3-(4-t-butyldimethylsilyloxybenzyl)-2-ethyl-2-hydroxysuccinic acid thus obtained was added acetic anhydride (5 mL), and the mixture was stirred at 115 *C for 3 h. The acetic anhydride was evaporated under reduced pressure, and the residue thus obtained was subjected to silica gel column chromatography 10 (toluene-ethyl acetate 19 : 1 -+ 16 : 1) to give the title compound as oil in an amount of 210 mg (2 step yield: 79 %). 'H NMR (CDCl 3 ) 6 1.16 (t, J = 7.6 Hz, 3H), 2.30 (s, 3H), 2.51 (q, J = 7.6 Hz, 2H), 3.78 (s, 2H), 7.05 (m, 2H), 7.50 (m, 2H). [0170] 15 Example 14: 3-Ethyl-2-(4-hydroxybenzyl)maleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-(4-acetoxybenzyl)-3-ethylmaleic anhydride as a solid (quantitative). 20 'H NMR (D 2 0) 6 0.83 (t, J = 7.6 Hz, 3H), 2.18 (q, J = 7.6 Hz, 2H), 3.40 (s, 2H), 6.66 (m, 2H), 6.99 (m, 2H); MS (FAB+) m/z 295 [(M+H)+]. [0171] Example 15 (Synthesis Example): 25 2,3-Dibenzylmaleic anhydride a) To a solution of (2S,3S)-2,3-dibenzylsuccinic acid (252 mg, 0.845 mmol) in anhydrous tetrahydrofuran (2.5 mL) were added N-methylmorpholine (90 mg, 0.89 mmol) and ethyl chloroformate (97 mg, 0.89 mmol) with stirring under ice 30 cooling, and the mixture was stirred at room temperature for 20 min. The reaction mixture was directly concentrated to dryness under reduced pressure to give 340 mg of (2S,3S)-2,3-dibenzylsuccinic anhydride(crude) as a solid. [0172] 35 b) To the suspension of (2S,3S)-2,3-dibenzylsuccinic anhydride thus obtained in anhydrous toluene (3.5 mL) were added triethylamine (361 mg, 2.54 mmol) and trimethylsilyltrifluoromethanesulfonic acid (565 mg, 2.54 mmol) with stirring under ice cooling, and the mixture was stirred at 90 *C for 2 h. To the reaction mixture with stirring under ice 5 cooling was added dropwise a solution of tetrabutylammonium bromide (2.7 mg, 0.00845 mmol) in dichloromethane(3 mL) and bromine (140 mg, 0.876 mmol), and the reaction mixture was stirred at the same temperature for 30 min. The reaction mixture was directly concentrated under reduced pressure and 10 the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 8 : 1) to give 59 mg of the title compound as a solid (2 step yield: 25%). 1H NMR (CDCl 3 ) 6 3.80 (s, 4H), 7.14 (m, 4H), 7.28 (m, 6H). [0173] 15 Example 16: 2,3-Dibenzylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 2,3-dibenzylmaleic anhydride as a solid (quantitative). 20 'H NMR (D 2 0) 6 3.59 (s, 4H), 7.10-7.21 (m, 10H); MS (FAB+) n/z 341 [(M+H)*]. [0174] Example 17 (Synthesis Example): 2-Benzyl-3-phenethylmaleic anhydride 25 a) Diisopropylamine (1.44 g, 14.3 mmol) was dissolved in anhydrous tetrahydrofuran (20 mL) under the atmosphere of argon, and a 1.54 M n-butyl lithium-hexane solution (8.8 mL, 13.6 mmol) was added with stirring under ice cooling. The mixture was stirred at 0 *C for 10 min, and then at -78 *C for 5 30 min.To the mixture, a solution of ethyl 3-phenylpropionate (2.14 g, 12.0 mmol) in anhydrous tetrahydrofuran (2 mL) was added at the same temperature over 6 min, and the reaction mixture was further stirred for 20 min. The cold solution of the enolate thus prepared above was added dropwise via a cannula 35 into a solution of ethyl 2-oxo-4-phenylbutyrate (2.05 g, 10 mmol) in anhydrous tetrahydrofuran (10 mL) having been cooled to -78 *C. After stirring at -78 0 C for 1 h, the reaction mixture was adjusted to pH 4 with acetic acid, and the temperature was raised to room temperature. The reaction solvent was evaporated under reduced pressure, and the 5 residue was diluted with ethyl acetate and water to separate the phases. The organic layer having been washed with brine was concentrated under reduced pressure. The residue obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 10 : 1) to give diethyl 10 3-benzyl-2-phenethyl-2-hydroxysuccinate as oil in amounts of 806 mg (low polar diastereomer, yield: 21%) and 412 mg (high polar diastereomer, yield: 13%), respectively. [0175] b) To a diethyl 3-benzyl-2-hydroxy-2-phenethylsuccinate (806 15 mg, 2.1 mmol) in 1,4-dioxane (8.0 mL) was added 1 M aqueous lithium hydroxide solution (8.4 mL, 8.4 mmol) at room temperature, and the mixture was stirred at 60 *C for 48 h. The reaction mixture was quenched and adjusted to pH 1 with 1 M hydrochloric acid with stirring under ice cooling. The solvent 20 was evaporated under reduced pressure, and the residue thus obtained was dissolved in ethyl acetate, and washed with water and brine. The organic layer was concentrated under reduced pressure and dried in vacuum to give 651 mg (crude) of 3-benzyl-2-hydroxy-2-phenethylsuccinic acid 25 [0176] c) To the crude 3-benzyl-2-hydroxy-2-phenethylsuccinic acid obtained was added acetic anhydride (2.5mL), and the mixture was stirred at 115 *C for 3 h. The acetic anhydride was evaporated under reduced pressure, and the residue thus 30 obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 10 : 1) to give 180 mg of the title compound as a solid (2 step yield: 81%). 'H NMR (CDCl 3 ) 6 2.73 (m, 2H), 2.82 (m, 2H), 3.51 (s, 2H), 7.09 (m, 4H), 7.22-7.32 (m, 6H). 35 [0177] Example 18: 2-Benzyl-3-phenethylmaleic acid disodium In the same manner as Example 11, the title compound was 5 obtained from 2-benzyl-3-phenethylmaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 2.44 (m, 2H), 2.53 (m, 2H), 3.44 (s, 2H), 7.07-7.23 (m, 10H); MS(FAB+) m/z 355 [(M+H)*]. [0178] 10 Example 19: 2,3-Diphenethylmaleic acid diethyl ester In the same manner as Example 3, the title compound was obtained from ethyl 2-bromo-4-phenylbutyrate as a solid (yield: 70%). 15 'H. NMR (CDCI 3 ) 6 1.32 (t, J = 7.2 Hz, 6H), 2.51-2.67 (m, 8H), 4.22 (q, J = 7.2 Hz, 4H), 7.14-7.30 (m, 10H). [0179] Example 20: 2,3-Diphenethylmaleic acid disodium 20 In the same manner as Example 2, the title compound was obtained from 2,3-diphenethylmaleic acid diethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 2.30 (m, 4H), 2.49 (m, 4H), 7.08-7.23 (m, 10H); MS (FAB+) m/z 369 [(M+H)+]. 25 [0180] Example 21 (Synthesis Example): 2-Isopropyl-3-methylmaleic anhydride a) To anhydrous tetrahydrofuran (5 mL) was added diisopropylamine (759 mg, 7.5 mmol) under nitrogen stream, 30 and the mixture was cooled to -78 *C. To the solution was added a 1.58 M n-butyl lithium-hexane solution (4.3 mL, 6.8 mmol), and the mixture was stirred at -78 *C for 5 min, and then at 0 *C for 15 min. The solution of lithium diisopropylamide tetrahydrofuran was cooled to -78 0 C, and a solution of benzyl 35 propionate (821 mg, 5.0 mmol) in anhydrous tetrahydrofuran (10 mL) was added dropwise over 1 h, and the reaction mixture was further stirred at -78 *C for 1 h. The enolate thus prepared was added via a cannula to a solution of benzyl 3-methyl-2-ketobutyrate (1.24 g, 6.0 mmol) in anhydrous tetrahydrofuran (5 mL) having been cooled to -78 *C. After 5 stirring at -78 *C for 2 h, the reaction mixture was diluted with a 10% aqueous citric acid solution, and extracted with ethyl acetate. The organic layer was washed with 1 M hydrochloric acid, saturated aqueous sodium hydrogen carbonate and brine, dried over anhydrous magnesium sulfate, and filtered. The 10 solvent was evaporated under reduced pressure, and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 9 : 1) to give 1.22 g of dibenzyl 2-isopropyl-2-hydroxy-3-methylsuccinate as oil (yield: 66%) in the form of a diastereomer mixture. 15 [0181] b) The dibenzyl 2-isopropyl-2-hydroxy-3-methylsuccinate thus obtained was dissolved in tetrahydrofuran (25 mL), and the solution was stirred in the presence of 10% palladium/carbon (120 mg) under hydrogen stream at room temperature for 15 h. 20 The catalyst was removed by filtration, and the filtrate was concentrated under reduced pressure to give 748 mg of 2-hydroxy-2-isopropyl-3-methylsuccinic acid as oil (quantitative). [0182] 25 c) The 2-hydroxy-2-isopropyl-3-methylsuccinic acid thus obtained was dissolved in acetic anhydride (30 mL), and stirred at 110 *C for 15 h. The acetic anhydride was evaporated under reduced pressure, and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 30 19 : 1) to give 203 mg of the title compound as oil (yield: 40%). 1 H (CDCl 3 ) 6 1.30 (d, J = 7.1 Hz, 6H), 2.10 (s, 3H), 2.92-3.03 (m, 1H); MS (EI) m/z 154 (M*). [0183] 35 Example 22: 2-isopropyl-3-methylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-isopropyl-3-methylmaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.91 (d, J = 6.9 Hz, 6H), 1.63 (s, 3H), 5 2.55-2.65 (m, 1H); MS (FAB+) m/z 217 [(M+H)*]. [0184] Example 23 (Synthesis Example): 3-Ethyl-2-isopropylmaleic anhydride In the same manner as Example 10, the title compound was 10 obtained from ethyl butyrate and ethyl 3-methyl-2-ketobutyrate as a oil (yield: 5 0 %). 'H NMR (CDCl 3 ) 6 1.15 (t, J = 7.6 Hz, 3H), 1.30 (d, J = 6.8 Hz, 6H), 2.54 (q, J= 7.6 Hz, 2H), 2.93-3.04 (m, 1H); MS (EI) m/z 169 [(M+H)*]. 15 [0185] Example 24: 3-ethyl-2-isopropylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 3-ethyl-2-isopropylmaleic anhydride as a solid 20 (quantitative). 'H NMR (D 2 0) 6 0.87 (t, J = 7.5 Hz, 3H), 0.91 (d, J = 6.2 Hz, 6H), 2.09 (q, J =7.5 Hz, 2H), 2.57-2.68 (m, 1H); MS (FAB+) m/z 231 [(M+ H)+]. [0186] 25 Example 25 (Synthesis Example): 2,3-Diisopropylmaleic anhydride In the same manner as Example 21, the title compound was obtained from benzyl 3-methylbutyrate and benzyl 3-methyl-2-ketobutyrate as a solid (yield: 68%). 30 NMR (CDCl 3 ) 6 1.30 (d, J = 6.9 Hz, 12H), 2.98-3.09 (m, 2H); MS (FAB+) m/z 183 [(M+H)*]. [0187] Example 26: 2,3-Diisopropylmaleic acid disodium 35 In the same manner as Example 11, the title compound was obtained from 2,3-diisopropylmaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.68 (d, J = 6.8 Hz, 12H), 2.32-2.42 (m, 2H); MS (FAB+) m/z 245 [(M+H)*]. [0188] 5 Example 27 (Synthesis Example): 3-Benzyl-2-isopropylmaleic anhydride In the same manner as Example 10, the title compound was obtained from ethyl phenylpropionate and ethyl 3-methyl-2-ketobutyrate as oil (yield: 27%). 10 'H NMR (CDCI 3 ) 6 1.27 (d, J = 6.9 Hz, 6H), 3.00-3.10 (m, 1H), 3.80 (s, 2H), 7.20-7.35 (m, 5H); MS (EI) m/z 231 [(M+H)*]. [0189] Example 28: 3-Benzyl-2-isopropylmaleic acid disodium 15 In the same manner as Example 11, the title compound was obtained from 3-benzyl-2-isopropylmaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.91 (d, J = 7.0 Hz, 6H), 2.65-2.76 (m, 1H), 3.53 (s, 2H), 7.06-7.23 (m, 5H); MS (FAB+) m/z 293 [(M+ 20 H)*]. [0190] Example 29 (Synthesis Example): 2-isopropyl-3-(2-methylphenyl)methylmaleic anhydride In the same manner as Example 21, the title compound was 25 obtained from benzyl 3-(2-methyl)phenylpropionate and benzyl 3-methyl-2-ketobutyrate as oil (yield: 60%). 'H NMR (CDCl 3 ) 6 1.22 (d, J = 6.1 Hz, 6H), 2.35 (s, 3H), 2.83-2.94 (m, 1H), 3.79 (s, 2H), 7.00 (d, J = 6.9 Hz, 1H), 7.12-7.20 (m, 3H); MS (EI) m/z 244 (M+). 30 [0191] Example 30: 2-Isopropyl-3-(2-methylphenyl)methylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-isopropyl-3-(2-methylphenyl)methylmaleic 35 anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.89 (d, J = 7.1 Hz, 6H), 2.19 (s, 3H), 2.54-2.65 (m, 1H), 3.47 (s, 2H), 7.00-7.15 (m, 4H); MS (FAB+) m/z 307 [(M+-H)*]. [0192] Example 31 (Synthesis Example): 5 2-Cyclopentyl-3-ethylmaleic anhydride In the same manner as Example 21, the title compound was obtained from benzyl cyclopentylacetate and benzyl 2-ketobutyrate as oil (yield: 63%). 'H NMR (CDCl 3 ) 6 1.18 (t, J = 7.5 Hz, 3H), 1.65-1.95 (m, 8H), 10 2.49 (q, J = 7.5 Hz, 2H), 2.92-3.03 (m, 1H); MS (EI) m/z 195 [(M+H)*]. [0193] Example 32: 2-Cyclopentyl-3-ethylmaleic acid disodium 15 In the same manner as Example 11, the title compound was obtained from 2-cyclopentyl-3-ethylmaleic anhydride as a solid (quantitative). 1H NMR (D 2 0) 6 0.80 (t, J = 7.3 Hz, 3H), 1.24-1.68 (m, 8H), 2.10 (q, J = 7.3 Hz, 2H), 2.57-2.67 (m, 1H); MS (FAB+) m/z 20 257 [(M+H)*]. [0194] Example 33 (Synthesis Example): 2-Cyclopentyl-3-isopropylmaleic anhydride In the same manner as Example 21, the title compound was 25 obtained from benzyl cyclopentylacetate and benzyl 3-methyl-2-ketobutyrate as oil (yield: 79%). 'H NMR (CDCI 3 ) 6 1.30 (d, J = 7.1 Hz, 6H), 1.65-1.95 (m, 8H), 2.96-3.08 (m, 2H); MS (EI) m/z 208 (M*). [0195] 30 Example 34: 2-Cyclopentyl-3-isopropylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-cyclopentyl-3-isopropylmaleic anhydride as a solid (quantitative). 35 'H NMR (D 2 0) 6 0.92 (d, J = 6.8 Hz, 6H), 1.26-1.70 (m, 8H), 2.56-2.68 (m, 2H); MS (FAB+) m/z 271 [(M+H)*].

[0196] Example 35 (Synthesis Example): Benzyl 2-keto-2-cyclopentylacetate a) A 0.5 M potassium bistrimethylsilylamide-toluene solution 5 (67.2 mL, 33.6 mmol) was added to anhydrous tetrahydrofuran (30 mL), and the mixture was cooled to -78 *C under nitrogen stream. To this mixture was added dropwise a solution of benzyl cyclopentylacetate (6.11 g, 28 mmol) in anhydrous tetrahydrofuran (30 mL) over 1 h. After the mixture was stirred 10 at -78 *C for 30 min, a solution of Davis oxaziridine reagent (8.78g, 33.6 mmol) in anhydrous tetrahydrofuran (30 mL) was added dropwise over 1 h. The reaction mixture, which had been stirred at -78 *C for 1 h, was diluted with a saturated aqueous ammonium chloride solution and extracted with diethyl ether. 15 The organic layer was washed with water and brine, dried over anhydrous magnesium sulfate, filtered, and the organic solvent in the filtrate was evaporated under reduced pressure. The residue was subjected to silica gel column chromatography (hexane - ethyl acetate 9 : 1) to give 6.157g of benzyl 20 2-cyclopentyl-2-hydroxyacetate (yield: 94%). 'H NMR (CDCl3) 6 1.36-1.74 (m, 8H), 2.19-2.3 (m, 1H), 2.70 (d, J = 6.5 Hz, 1H), 4.17 (dd, J = 4.9, 6.5 Hz, 1H), 5.19 (d, J = 12.2 Hz, 1H), 5.23 (d, J = 12.2 Hz, 1H), 7.30-7.45 (m, 5H). [0197] 25 b) To a solution of benzyl 2-cyclopentyl-2-hydroxyacetate (1.76 g, 7.5 mmol) in dichloromethane (30 mL) was added Dess-Martin reagent (5 g, 11.8 mmol), and the mixture was stirred at room temperature for 15 h. The reaction mixture was diluted with 10% sodium thiosulfate, and extracted with diethyl 30 ether. The organic layer was washed with water, saturated aqueous sodium hydrogen carbonate, and brine, dried over anhydrous magnesium sulfate, filtered, and the organic solvent in the filtrate was evaporated under reduced pressure. The residue was subjected to silica gel column chromatography 35 (hexane - ethyl acetate 9 : 1) to give 1.383 g of the title compound as oil (yield: 79%).

'H NMR (CDCl 3 ) 6 1.58-1.92 (m, 8H), 3.44-3.53 (m, 1H), 5.28 (s, 2H), 7.34-7.42 (m, 5H); MS (EI) m/z 232 (M*). [0198] Example 36 (Synthesis Example): 5 3-Benzyl-2-cyclopentylmaleic anhydride In the same manner as Example 21, the title compound was obtained from benzyl 3-phenylpropionate and benzyl 2-cyclopentyl-2-ketoacetate as oil (yield: 42%). 'H NMR (CDCl 3 ) 6 1.60-1.95 (m, 8H), 3.00-3.10 (m, 1H), 3.80 10 (s, 2H), 7.18-7.34 (m, 5H); MS (EI) m/z 256 (M). [0199] Example 37: 3-benzyl-2-cyclopentylmaleic acid disodium In the same manner as Example 11, the title compound was 15 obtained from 3-benzyl-2-cyclopentylmaleic anhydride as a solid (quantitative). 1H NMR (D 2 0) 6 1.28-1.66 (m, 8H), 2.64-2.74 (m, 1H), 3.54 (s, 2H), 7.06-7.22 (m, 5H); MS (FAB+) m/z 319 [(M+H)*]. [0200] 20 Example 38 (Synthesis Example): 2,3-Dicyclopentylmaleic anhydride In the same manner as Example 21, the title compound was obtained from benzyl cyclopentylacetate and benzyl 2-cyclopentyl-2-ketoacetate as a solid (yield: 60%). 25 'H NMR (CDCl 3 ) 6 1.60-1.90 (m, 16H), 2.97-3.07 (m, 2H); MS (EI) m/z 234 (M). [0201] Example 39: 2,3-Dicyclopentylmaleic acid disodium 30 In the same manner as Example 11, the title compound was obtained from 2,3-dicyclopentylmaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 1.26-1.68 (m, 16H), 2.57-2.67 (m, 2H); MS (FAB+) m/z 297 [(M+H)*]. 35 [0202] Example 40 (Synthesis Example): 2-(2,3-Dihydro-1H-inden-2-yl)-3-isopropylmaleic anhydride In the same manner as Example 21, the title compound was obtained from benzyl 2-(2,3-dihydro-1H-inden-2-yl)acetate and benzyl 3-methyl-2-ketobutyrate as oil (yield: 62%). 5 1H NMR (CDCl 3 ) 6 1.30 (d, J = 7.1 Hz, 6H), 2.98-3.08 (m, 1H), 3.14 (dd, J = 8.6, 15.1 Hz, 2H), 3.33 (dd, J = 10.0, 15.1 Hz, 2H), 3.65-3.75 (m, 1H), 7.18-7.26 (m, 4H); MS (EI) m/z 256 (M*). [0203] 10 Example 41: 2-(2,3-Dihydro-1H-inden-2-yl)-3-isopropylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-(2,3-dihydro-1H-inden-2-yl)-3-isopropylmaleic anhydride as a solid (quantitative). 15 1H NMR (D 2 0) 6 0.95 (d, J = 7.1 Hz, 6H), 2.62-2.73 (m, 1H), 2.84-2.96 (m, 4H), 3.26-3.37 (m, 1H), 7.02-7.07 (m, 2H), 7.12-7.16 (m, 2H); MS (FAB+) m/z 319 [(M+H)*]. [0204] Example 42 (Synthesis Example): 20 2-Cyclohexyl-3-isopropylmaleic anhydride In the same manner as Example 21, the title compound was obtained from benzyl cyclohexylacetate and benzyl 3-methyl-2-ketobutyrate as oil (yield: 57%). 'H NMR (CDCl 3 ) 6 1.30 (d, J = 7.0 Hz, 6H), 1.60-1.90 (m, 25 10H), 2.62-2.72 (m, 1H), 3.00-3.12 (m, 1H); MS (EI) m/z 222 (M+). [0205] Example 43: 2-Cyclohexyl-3-isopropylmaleic acid disodium 30 In the same manner as Example 11, the title compound was obtained from 2-cyclohexyl-3-isopropylmaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.91 (d, J = 6.8 Hz, 6H), 0.96-1.28 (m, 5H), 1.47-1.59 (m, 5H), 2.15-2.26 (m, 1H), 2.56-2.64 (m, 1H); MS 35 (FAB+) m/z 285 [(M+H)+]. [0206] Example 44 (Synthesis Example): Trans-4-benzyloxycarbonylmethyl-1-t-butyldimethylsilyloxycyclo hexane and cis-4-benzyloxycarbonylmethyl-1-t-butyldimethylsilyloxycyclohe 5 xane a) To a solution of 1,4-cyclohexanedionemonoethyleneacetal (5.02 g, 32.1 mmol) in toluene (150 mL) were added benzyl (triphenylphospholanylidene)acetate (23.7 g, 57.8 mmol) and benzoic acid (832.5 mg, 6.82 mmol) at room temperature, and 10 the mixture was vigorously stirred at 95 *C for 2 days. The reaction mixture was directly concentrated under reduced pressure, and the residue obtained was diluted with hexane (300 mL) and diethyl ether (150 mL), and vigorously stirred for 10 min. The reaction mixture was filtered, and the residue 15 obtained by concentrating the filtrate under reduced pressure was subjected to silica gel column chromatography (hexane ethyl acetate 6 1 -+ 4 : 1) to give 8.53 g of 4-benzyloxycarbonylmethylidenecyclohexane-ethylene acetal as oil (yield: 92%). 20 [0207] b) To a solution of 4-Benzyloxycarbonylmethylidenecyclohexane-ethylene acetal (8.19 g, 28.4 mmol) in methanol were added diphenyl sulfide (52 mg, 0.28 mmol) and 10% palladium/carbon (1.74 g), and 25 the mixture was vigorously stirred under hydrogen atmosphere at room temperature for 40 h. The reaction mixture was filtered, and the residue obtained by concentrating the filtrate under reduced pressure was subjected to silica gel column chromatography (hexane - ethyl acetate3:1) to give 7.62 g of 30 4-benzyloxycarbonylmethylcyclohexanone ethylene acetal as oil (yield: 92%). [0208] c) To a solution of 4-benzyloxycarbonylmethylcyclohexanone ethylene acetal (7.62 g, 26.2 mmol) in acetone (235 mL)-water 35 (10 mL) was added p -toluenesulfonic acid monohydrate (221 mg, 1.16 mmol), and the mixture was stirred at 55 *C for 10 h.

The reaction mixture was adjusted to pH 5 with sodium hydrogen carbonate with stirring under ice cooling, and concentrated under reduced pressure. The residue obtained by azeotropic distillation with the addition of toluene was subjected 5 to silica gel column chromatography (hexane - ethyl acetate 4 : 1) to give 5.57 g of benzyl 2-(4-oxocyclohexyl)acetate as oil (yield: 86%). [0209] d) To a solution of benzyl 2-(4-oxocyclohexyl)acetate (2.64 g, 10 10.7 mmol) in methanol (50 mL) was added sodium borohydride (393 mg, 10.4 mmol) with stirring under ice cooling. After stirring at the same temperature for 2 h, the reaction mixture was adjusted to pH 4 with acetic acid (1 mL). The reaction mixture was concentrated under reduced pressure, and the 15 residue thus obtained was diluted with ethyl acetate and water to separate the layers. The aqueous layer was extracted again with ethyl acetate, and the combined organic layer was concentrated under reduced pressure, and the residue was subjected to silica gel column chromatography (hexane - ethyl 20 acetate 3 : 1 -+ 2 : 1 - 1 : 1) to give 2.20 g of trans-4-benzyloxycarbonylmethyl-1-hydroxycyclohexane (yield: 82%) and 540 mg of cis-4-benzyloxycarbonylmethyl-1-hydroxycyclohexane (yield: 20%) as oil. 25 [0210] e) To a solution of trans-4-benzyloxycarbonylmethyl-1-hydroxycyclohexane (2.20 g, 8.86 mmol) in dimethylformamide (31 mL) were added imidazole (953mg, 14.0 mmol) and t-butyldimethylsilyl chloride 30 (1.60 g, 10.6 mmol) at room temperature, and the mixture was stirred at 60 *C for 3 h. The reaction mixture was diluted with water (70 mL) and hexane (200 mL) with stirring under ice cooling to separate the phases. The aqueous layer was extracted again with hexane, and the combined organic layer 35 was concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 50 : 1) to give 2.96 g of trans-4-benzyloxycarbonylmethyl-1-t-butyldimethylsilyloxycyclo hexane as oil (yield: 92%). 'H NMR (CDCI 3 ) 6 0.04 (s, 6H), 0.89 (s, 9H), 1.01 (m, 2H), 5 1.31 (m, 2H), 1.57-1.84 (m, 5H), 2.23 (d, J = 6.6 Hz, 2H), 3.50 (m, 1H), 5.11 (s, 2H), 7.30-7.38 (m, 5H). [0211] f) In the same manner as the step e), cis-4-benzyloxycarbonylmethyl-1-t-butyldimethylsilyloxycyclohe 10 xane as oil (yield: 89%) from cis-4-benzyloxycarbonylmethyl-1-hydroxycyclohexane. 'H NMR (CDCl 3 ) 6 0.02 (s, 6H), 0.87 (s, 9H), 1.44-1.49 (m, 6H), 1.52-1.62 (m, 2H), 1.85 (m, 1H), 2.28 (d, J = 7.3 Hz, 2H), 3.92 (m, 1H), 5.11 (s, 2H), 7.30-7.38 (m, 5H). 15 [0212] Example 45 (Synthesis Example): 2-(Trans-4-hydroxycyclohexyl)-3-isopropylmaleic anhydride a) To a solution of diisopropylamine (418 mg, 4.15 mmol) in anhydrous tetrahydrofuran (6 mL) under the atmosphere of 20 argon was added a 1.58 M n-butyl lithium-hexane solution (2.5 mL, 5.4 mmol) with stirring under ice cooling, and the mixture was stirred at 0 *C for 10 min, and then at -78 *C for 5 min. A solution of trans-4-benzyloxycarbonylmethyl-1-t-butyldimethylsilyloxycyclo 25 hexan (1.36 g, 3.76 mmol) in anhydrous tetrahydrofuran (7 mlL) was added dropwise at the same temperature over 7 min, and the reaction mixture was further stirred for 15 min. The cold enolate solution thus prepared was added dropwise via canula to a solution of benzyl 3-methyl-2-ketobutyrate (776 mg, 3.76 30 mmol) in anhydrous tetrahydrofuran (4 mL) having been cooled to -78 *C. After stirring at -78 *C for 1 h, the reaction mixture was adjusted to pH 4 with acetic acid, and the temperature was raised to room temperature. The reaction solvent was evaporated under reduced pressure, and the residue was diluted 35 with ethyl acetate and water to separate the phases for extraction. The aqueous layer was extracted again with ethyl acetate, and the combined organic layer was concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 20 : 1) to give dibenzyl 5 3-(trans-4-t-butyldimethylsilyloxycyclohexyl)-2-hydroxy-2-isopr opylsuccinate as oil in amounts of 629 mg (low polar diastereomer, yield: 2 9%) and 801 mg (high polar diastereomer, yield: 3 7 %), respectively. [0213] 10 b) To a solution of dibenzyl 3-(trans-4-t-butyldimethylsilyloxycyclohexyl)-2-hydroxy-2-isopr opylsuccinate (801 mg, 1.41 mmol) in ethanol (13 mL) was added 10% palladium/carbon (251 mg, 50% water containing product), and the mixture was stirred under hydrogen stream at 15 room temperature for 20 h. The catalyst was removed by filtration, and the filtrate was concentrated to dryness under reduced pressure to give 514 mg of 3-(trans-4-t-butydimethylsilyloxy)-2-hydroxy-2-isopropylsuccini c acid as oil (yield: 94%). 20 [0214] c) 3-(Trans-4-t-butyldimethylsilyloxycyclohexyl) 2-hydroxy-2-isopropylsuccinic acid (510 mg, 1.31 mmol) was dissolved in acetic anhydride (5.5 mL), and the solution was stirred at 120 *C for 18 h. The acetic anhydride was evaporated 25 under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 50 : 1) to give 293 mg of 2-(trans-4-t-butyldimethylsilyloxycyclohexyl)-3-isopro pylmaleic anhydride as oil (yield: 63%). 30 [0215] d) To a solution of 2-(trans-4-t-butyldimethylsilyloxycyclohexyl)-3-isopropylmaleic anhydride (263 mg, 0.745 mmol) in ethanol (7 mL) was added concentrated. hydrochloric acid (83 mg, 0.84 mmol), and the 35 mixture was left standing at 50 *C for 30 min. The reaction mixture was directly concentrated, and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 1 : 1) to give 179 mg (quantitative) of the title compound as a solid. 1H NMR (CDCI 3 ) 5 1.31 (d, J = 6.8 Hz, 6H), 1.38 (m, 2H), 1.55 5 (br s, 1H), 1.70 (m, 2H), 1.93 (m, 2H), 2.11 (m, 2H), 2.63 (m, 1H), 3.03 (m, 1H), 3.71 (m, 1H). [0216] Example 46: 2-(trans-4-Hydroxycyclohexyl)-3-isopropylimaleic acid disodium 10 In the same manner as Example 11, the title compound was obtained from 2-(trans-4-hyd roxycyclohexyl)-3-isopropylmaleic anhydride as a solid (quantitative). 1H NMR (D 2 0) 6 0.93 (d, J = 7.1 Hz, 6H), 1.18 (m, 2H), 1.35 (m, 2H), 1.57 (m, 2H), 1.83 (m, 2H), 2.21 (m, 1H), 2.60 15 (septet, J = 7.1 Hz, 1H), 3.46 (m, 1H); MS (FAB+) m/z 301 [(M+H)*]. [0217] Example 47 (Synthesis Example): 2-(Cis-4-hydroxycyclohexyl)-3-isopropylmaleic anhydride 20 In the same manner as Example 45, the title compound was obtained from cis-4-benzyloxycarbonylmethyl-1-t-butyldimethylsilyloxycyclohe xane as a solid (yield: 42%). 'H NMR (CDCl 3 ) 5 1.31 (d, J = 6.8 Hz, 6H), 1.40 (br s, 1H), 25 1.48 (m, 2H), 1.59 (m, 2H), 1.92 (m, 2H), 2.20 (m, 2H), 2.70 (m, 1H), 3.11 (septet, J = 6.8 Hz, 1H), 4.15 (m, 1H). [0218] Example 48: 2-(cis-4-Hydroxycyclohexyl)-3-isopropylmaleic acid disodium 30 In the same manner as Example 11, the title compound was obtained from 2-(cis-4-hydroxycyclohexyl)-3-isopropylmaleic anhydride as a solid (quantitative). 'H NMR (D20) 6 0.92 (d, J = 7.0 Hz, 6H), 1.32 (m, 2H), 1.42-1.60 (m, 4H), 1.65(m, 2H), 2.28 (m, 1H), 2.61 (septet, J 35 = 7.0 Hz, 1H), 3.88 (m, 1H); MS (FAB+) m/z 301 [(M+H)*].

[0219] Example 49 (Synthesis Example): Benzyl 2-(tetrahydropyran-4-yl)acetate To a solution of 2-(tetrahydropyran-4-yl)acetic acid (325 mg, 5 2.26 mmol) in anhydrous dichloromethane(lOmL) were added benzyl alcohol (439 mg, 2.94 mmol), 1-ethyl-3-(3-dimethylammoniumpropyl)carbodiimide hydrochloride (564 mg, 2.94 mmol) and triethylamine (297 mg, 2.94 mmol) at room temperature, and the mixture was stirred 10 for 12 h. The reaction mixture was diluted with water (1 mL)to separate the phases, and the organic layer was further washed with water (1 mL) and brine (1 mL). The organic layer was concentrated under reduced pressure, and the residue thus obtained was subjected to silica gel column chromatography 15 (hexane - ethyl acetate 7 : 1) to give 172 mg of the title compound as oil (yield: 32%). 'H NMR (CDCl 3 ) 6 1.34 (m, 2H), 1.63 (m, 2H), 2.02 (m, 1H), 2.30 (d, I = 7.1 Hz, 2H), 3.39 (m, 2H), 3.93 (m, 2H), 5.12 (s, 2H), 7.31-7.40 (m, 5H). 20 [0220] Example 50 (Synthesis Example): 3-Isopropyl-2-(tetrahydropyran-4-yl)maleic anhydride a) To a solution of diisopropylamine (122 mg, 1.21 mmol) in anhydrous tetrahydrofuran (2 mL) under the atmosphere of 25 argon was added a 1.58 M n-butyl lithium-hexane solution (703 pL, 1.11 mmol) with stirring under ice cooling, and the mixture was stirred at 0 *C for 10 min and then at -78 *C for 5 min. A solution of benzyl 2-(tetrahydropyran-4-yl)acetate (250 mg, 1.07 mmol) in anhydrous tetrahydrofuran (1 mL) was added 30 dropwise over 6 min at the same temperature, and the reaction mixture was further stirred for 15 min. The cold enolate solution thus prepared was added dropwise via a cannula to a solution of benzyl a solution of benzyl 3-methyl-2-ketobutyrate (191 mg, 0.928 mmol) in anhydrous tetrahydrofuran (1 mL) solution 35 having been cooled to -78 *C. After stirring at -78 *C for 1 h, the reaction mixture was adjusted to pH 4 with acetic acid and raised to room temperature. The reaction solvent was evaporated under reduced pressure, and the residue was diluted with ethyl acetate and water to separate the phases for extraction. The aqueous layer was extracted again with ethyl 5 acetate, and the combined organic layer was concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 7 1) to give dibenzyl 2-hydroxy-2-isopropyl-3-(tetrahydro-2H-pyran-4-yl)succinate 10 as oil in amounts of 115 mg (low polar diastereomer, yield: 29%) and 158 mg (high polar diastereomer, yield: 39%), respectively. [0221] b) To a solution of dibenzyl 15 2-hydroxy-2-isopropyl-3-(tetrahydro-2H-pyran-4-yl)succinate (112 mg, 0.255 mmol) in ethanol (2.8 mL) was added 10% palladium/carbon (44 mg, 50% wet), and the mixture was stirred under hydrogen stream at room temperature for 2 h. The catalyst was removed by filtration, and the filtrate was 20 concentrated to dryness under reduced pressure to give 72 mg (quantitative) of 2-hydroxy-2-isopropyl-3-(tetrahydropyran-4-yl)succinic acid as a foam. [0222] 25 c) 2-Hydroxy-2-isopropyl-3-(tetrahydropyran-4-yl)succinic acid (70 mg, 0.27 mmol) was dissolved in acetic anhydride (1 mL), and the solution was left standing at 120 *C for 10 h. The acetic anhydride was evaporated under reduced pressure and the residue thus obtained was subjected to silica gel column 30 chromatography (hexane - ethyl acetate 3 : 1) to give 38 mg of the title compound as a solid (yield: 6 7 %). 1 H NMR (CDCl 3 ) 6 1.32 (d, J = 7.1 Hz, 6H), 1.54 (m, 2H), 2.16 (m, 2H), 2.94 (m, 1H), 3.10 (septet, J = 7.1 Hz, 1H), 3.45 (m, 2H), 4.08 (m, 2H). 35 [0223] Example 51: 3-Isopropyl-2-(tetrahydropyran-4-yl)maleic acid disodium In the same manner as Example 11, the title compound was 5 obtained from 3-isopropyl-2-(tetrahydropyran-4-yl)maleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.93 (d, J = 7.1 Hz, 6H), 1.42 (m, 2H), 1.61 (m, 2H), 2.55 (m, 1H), 2.62 (septet, J = 7.1 Hz, 1H), 3.38 (m, 2H), 3.84 (m, 2H); MS (FAB+) m/z 287 [(M+H)+]. 10 [0224] Example 52 (Synthesis Example): Benzyl 3-(3-pyridyl)propionate To a suspension of 3-(3-pyridyl)propionic acid hydrochloride (1.51 g, 10.0 mmol) in benzyl alcohol (3.1mL, 30 mmol) was 15 added p -toluenesulfonic acid monohydrate (181.8 mg, 0.96 mmol), and the mixture was stirred at 130 0 C for 20 h. The reaction mixture was diluted with ethyl acetate and a saturated aqueous sodium hydrogen carbonate solution was added to separate the phases. The aqueous layer was extracted again 20 with ethyl acetate, and the combined organic layer was concentrated with reduced pressure. The residue was subjected to silica gel column chromatography (hexane - ethyl acetatel : 1 -+ 1 : 3) to give 1.69 g of the title compound as oil (yield: 70%). 25 [0225] Example 53(Synthesis Example): 2-Isopropyl-3-[(pyridine-3-yl)methyl]maleic anhydride In the same manner as Example 50, the title compound was obtained from benzyl 3-(3-pyridyl)propionate and benzyl 30 3-methyl-2-ketobutyrate as oil in an amount of 122 mg (yield: 38%). 1H NMR (CDCI 3 ) 5 1.30 (d, J = 6.8 Hz, 6H), 3.07 (septet, J = 6.8 Hz, 1H), 3.82 (s, 2H), 7.28 (m, 1H), 7.59 (m, 1H), 8.53 (m, 2H). 35 [0226] Example 54: 2-Isopropyl-3-[(pyridine-3-yl)methyl]maleic acid disodium In the same manner as Example 11, the title compound was 5 obtained from 2-isopropyl-3-[(pyridine-3-yl)methyl]maleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.90 (d, J = 6.8 Hz, 6H), 2.68 (septet, J = 6.8 Hz, 1H), 3.55 (s, 2H), 7.24 (m, 1H), 7.62 (m, 1H), 8.19 (m, 1H), 8.26 (m, 1H); MS (FAB+) m/z 294[(M+H)+]. 10 [0227] Example 55: Dimethyl 3-methyl-2-[(piperidin-1-yl)methyl]maleate a) To a solution of dimethyl 2,3-dimethylmaleate (500 mg, 2.90 mmol)in carbon tetrachloride (11mL) were added 15 N-bromosuccinimide (803 mg, 4.51 mmol) and 2,2'-azobisisobutyronitrile (3.2 mg, 0.019 mmol) at room temperature, and the mixture was heated under reflux with stirring for 4 h. The reaction mixture was directly filtrated, and the filtrate was washed with water and brine. The residue 20 obtained by concentrating the organic layer under reduced pressure was subjected to silica gel column chromatography (hexane - ethyl acetate 20 : 1 -> 8 : 1) to give 191 mg of dimethyl 2-bromomethyl-3-methylmaleate (yield: 26%). [0228] 25 b) To a solution of dimethyl 2-bromomethyl-3-methylmaleate (32 mg, 0.13 mmol) in anhydrous dimethylformamide (450 pL) was added piperidine (21 mg, 0.25 mmol) at room temperature, and the mixture was left standing at 50 *C for 4 h. The reaction mixture was directly concentrated under reduced pressure and 30 the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 3 : 2) to give 3 mg of the title compound as oil (yield: 39%). 'H NMR (CDCl 3 ) 6 1.39 (m, 2H), 1.51 (m, 4H), 1.99 (s, 3H), 2.37 (m, 4H), 3.23 (s, 2H), 3.75 (s, 3H), 3.76 (s, 3H). 35 [0229] Example 56: 3-Methyl-2-[(piperidin-1-yl)methyl]maleic acid disodium In the same manner as Example 2, the title compound was 5 obtained from dimethyl 3-methyl-2-[(piperidin-1-yl)methyl]maleate as a solid (quantitative). 'H NMR (D 2 0) 6 1.26 (m, 2H), 1.38 (m, 4H), 1.74 (s, 3H), 2.33 (m, 4H), 3.10 (s,2H); MS (FAB+) m/z 272 [(M+H)*]. 10 [0230] Example 57: 2-[(4-Hydroxypiperidin-1-yl)methyl]-3-methylmaleic acid dimethyl ester In the same manner as Example 55, the title compound was 15 obtained from dimethyl 2,3-dimethylmaleate and 4-hydroxypiperidine as oil (yield: 43%). 1H NMR (CDCl 3 ) 6 1.35 (br s, 1H), 1.55 (m, 2H), 1.84 (m, 2H), 1.99 (s, 3H), 2.17 (m, 2H), 2.74 (m, 2H), 3.27 (s, 2H), 3.68 (in, 1H), 3.76 (s, 3H), 3.78 (s, 3H). 20 [0231] Example 58: 2-[(4-Hydroxypiperidin-1-yl)methyl]-3-methylmaleic acid disodium In the same manner as Example 2, the title compound was 25 obtained from 2-[(4-hydroxypiperidin-1-yl)methyl]-3-methylmaleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 1.34 (m, 2H,), 1.70 (m, 2H), 1.72 (s, 3H), 2.13 (m, 2H), 2.66 (m, 2H), 3.13 (s, 2H), 3.54 (m, 1H); MS 30 (FAB+) m/z 288 [(M+H)+]. [0232] Example 59 (Synthesis Example): 2-Hydroxymethyl-3-methylmaleic anhydride To 2-bromomethyl-3-methylimaleic anhydride (1.25 g, 6.10 35 mmol) was added 5 M aqueous sodium hydroxide solution (4 mL) at room temperature, and the mixture was stirred for 12 h.

The reaction mixture was adjusted to pH 1 with 5 M hydrochloric acid (5 mL), added with sodium chloride and extracted three times with ethyl acetate. The combined organic layer was concentrated under reduced pressure, and the residue 5 thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 2 : 1) to give 227 mg of the title compound as oil (yield: 26%). 1H NMR (CDCl 3 ) 6 2.21 (t, J = 1.1 Hz, 3H), 4.63 (q, J = 1.1 Hz, 2H). 10 [0233] Example 60: 2-Hydroxymethyl-3-methylmaleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-hydroxymethyl-3-methylmaleic anhydride as a 15 solid (quantitative). 'H NMR (D 2 0) 6 1.75 (s, 3H), 4.65 (s, 2H); MS (FAB+) m/z 205 [(M+H)+]. [0234] Example 61 (Synthesis Example): 20 3-Ethyl-2-methoxymaleic anhydride In the same manner as Example 17, the title compound was obtained from ethyl 2-ketobutyrate and methyl methoxyacetate as oil (yield: 37%). 'H NMR (CDCI3) 6 1.18 (t, J = 7.6 Hz, 3H), 2.45 (q, J = 7.6 Hz, 25 2H), 4.25 (s, 3H). [0235] Example 62: 3-Ethyl-2-methoxymaleic acid disodium In the same manner as Example 11, the title compound was 30 obtained from 3-ethyl-2-methoxymaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 0.82 (t, J = 7.5 Hz, 3H), 2.13 (q, J = 7.5 Hz, 2H), 3.39 (s, 3H); MS (FAB+) m/z 219 [(M+H)+]. [0236] 35 Example 63 (Synthesis Example): 3-Ethyl-2-methylthiomaleic anhydride In the same manner as Example 17, the title compound was obtained from ethyl 2-ketobutyrate and methyl thioethylacetate as oil (yield: 18%). 'H NMR (CDCI 3 ) 6 1.18 (t, J = 7.5 Hz, 3H), 2.49 (q, J = 7.5 Hz, 5 2H), 2.77 (s, 3H). [0237] Example 64: 3-ethyl-2-methylthiomaleic acid disodium In the same manner as Example 11, the title compound was 10 obtained form 3-ethyl-2- methylthiomaleic anhydride as a solid (quantitative). 1H NMR (D 2 0) 6 0.81 (t, J = 7.5 Hz, 3H), 2.05 (s, 3H), 2.22 (q, J = 7.5 Hz, 2H); MS (ESI+) m/z 235 [(M+H)*]. [0238] 15 Example 65 (Synthesis Example): 3-Ethyl-2-isopropylthiomaleic anhydride In the same manner as Example 17, the title compound was obtained from ethyl 2-ketobutyrate and isopropyl thioethylacetate as oil (yield: 26%). 20 1H NMR (CDCI 3 ) 6 1.17 (t, J = 7.6 Hz, 3H), 1.37 (d, J = 6.8 Hz, 6H), 2.49 (q, J= 7.6 Hz, 2H), 4.38 (septet, ) = 6.8 Hz, 1H). [0239] Example 66: 3-Ethyl-2-isopropylthiomaleic acid disodium 25 In the same manner as Example 11, the title compound was obtained from 3-ethyl-2-isopropylthiomaleic anhydride as a solid (quantitative). 'H NMR (D 2 0) 6 1.17 (t, J = 7.6 Hz, 3H), 1.37 (d, J = 6.8 Hz, 6H), 2.49 (q, J = 7.6 Hz, 2H), 4.38 (septet, J = 6.8 Hz, 1H); MS 30 (FAB+) m/z 263 [(M+H)*]. [0240] Example 67 (Synthesis Example): Ethyl 3

-{

4 -[(t-butyldiphenylsilyloxymethyl)phenyl]}propionate a) To a solution of 35 4 -(t-butyldiphenylsilyloxymethyl)benzaldehyde (3.26 g, 8.71 mmol) in benzene (80 mL) were added ethyl (triphenylphospholanylidene)acetate (3.19 g, 9.14 mmol) and benzoic acid (116.0 mg, 0.950 mmol) at room temperature, and the mixture was heated under reflux with stirring for 2 h. The reaction mixture was directly concentrated under reduced 5 pressure, and the residue thus obtained was diluted with hexane (80 mL) and diethyl ether (40 mL) and stirred for 8 h. Precipitated solids were filtered, and the residue obtained by concentrating the filtrate under reduced pressure was subjected to silica gel column chromatography (hexane - ethyl acetate 10 25 1) to give 13.47 g of ethyl (E)-3-{4-[(t-butyldiphenylsilyloxy)methyl]phenyl}acrylate as oil (yield: 90%). [0241] b) To a solution of ethyl 15 (E)-{3-{[(t-butyldiphenylsilyloxy)methyl]phenyl}acrylate (3.47 g, 7.80 mmol) in methanol (70 mL) were added diphenyl sulfide (14 mg, 0.078 mmol) and 10% palladium/carbon (1.06 g), and the mixture was vigorously stirred at room temperature under hydrogen atmosphere for 12 h. The reaction mixture was 20 filtered, and the residue obtained by concentrating the filtrate under reduced pressure was subjected to silica gel column chromatography (hexane - ethyl acetate 25 : 1 - 10 : 1) to give 3.21 g of the title compound as oil (yield: 92%). 'H NMR (CDCI 3 ) 6 1.09 (s, 9H), 1.24 (t, J = 7.2 Hz, 3H), 2.62 25 (t, J = 7.8 Hz, 2H), 2.95 (t, J = 7.8 Hz, 2H), 4.13 (q, J = 7.2 Hz, 2H), 4.74 (s, 2H), 7.17 (d, J = 8.0 Hz, 2H), 7.27 (d, J = 8.0 Hz, 2H), 7.17 (d, J = 8.0 Hz, 2H), 7.35-7.43 (m, 6H), 7.69 (m, 4H). [0242] 30 Example 68 (Synthesis Example): 2-[4-(Hydroxymethyl)benzyl]-3-isopropylmaleic anhydride a) To diisopropylamine (1.06 g, 0.763 mmol) dissolved in anhydrous tetrahydrofuran (15 mL) under the atmosphere of argon was added 1.60 M n-butyl lithium-hexane solution (4.5 35 mL, 7.16 mmol) with stirring under ice cooling, and the mixture was stirred at 0 *C for 10 min, and then at -78 *C for 5 min. To the mixture, ethyl 3-{4-[(t-butyldiphenylsilyloxymethyl)phenyl]}propionate (3.20 g, 7.16 mmol) dissolved in anhydrous tetrahydrofuran (20 mL) was added dropwise at the same temperature over 15 min, and 5 the reaction mixture was further stirred for 20 min. The cold solution of the enolate thus prepared was added dropwise via a cannula to a solution of ethyl 3-methyl-2-ketobutyrate (1.14 g, 7.88 mmol) in anhydrous tetrahydrofuran (5.5 mL) having been cooled to -78 *C. After stirring at -78 *C for 1 h, the reaction 10 mixture was adjusted to pH 4 with acetic acid, and raised up to room temperature. The reaction solvent was evaporated under reduced pressure, and the residue was diluted with ethyl acetate and water to separate the phases. After the organic layer was washed with brine, the mixture was concentrated 15 under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 10 1) to give diethyl 3-{4-[(t-butyldiphenylsilyloxymethyl)phenyl]}-2-isopropyl-2-hy droxysuccinate as oil in amounts of 962 mg (low polar 20 diastereomer, yield: 23%) and 999 mg (high polar diastereomer, yield: 24%), respectively. [0243] b) To a solution of diethyl 3-{4-[(t-butyldiphenylsilyloxymethyl)phenyl]}-2-isopropyl-2-hy 25 droxysuccinate (1.32 g, 1.59 mmol) in tetrahydrofuran (26 mL) was added a 1 M tetrabutylammonium fluoride-tetrahydrofuran solution (2.45 mL, 2.45 mmol) at room temperature, and the mixture was left standing at room temperature for 2 h. The reaction mixture was directly concentrated under reduced 30 pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 2 : 1 -+ 1 1 ) to give 700 mg of diethyl 2-isopropyl-3-{4-[(hydroxymethyl) phenyl] }-2-hydroxysuccinate as oil (yield: 89%). 35 [0244] c) To a solution of diethyl 2-isopropyl-3-{4-[(hydroxymethyl) phenyl] }-2-hydroxysucci nate thus obtained (700 mg, 1.99 mmol) in 1,4-dioxane(6.0 mL) was added 1 M aqueous lithium hydroxide solution (4.0 mL, 4.0 mmol) at room temperature, and the mixture was stirred at 5 60 *C for 16 h. Furthermore, 5 M aqueous sodium hydroxide solution (16.5 mL, 82.5 mmol) was added, and the reaction mixture was stirred at 90 *C for 24 h. After the reaction mixture was left cooling and stirred under ice cooling together with 5 N hydrochloric acid (19 mL), the reaction mixture was 10 concentrated to dryness to give a crude 2-isopropyl-3-{4-[(hydroxymethyl) phenyl] }-2-hydroxysuccinic acid. [0245] d) To the crude 15 2-isopropyl-3-{4-[(hydroxymethyl)phenyl]}-2-hydroxysuccinic acid obtained in the step c) described above was added acetic anhydride (20 mL), and the mixture was stirred at 140 *C for 2 h. The reaction mixture was directly filtrated, and the residue obtained by concentrating the filtrate under reduced pressure 20 was subjected to silica gel column chromatography (hexane ethyl acetate 6 1) to give 302 mg of 2-isopropyl-3-{4-((hydroxymethyl)phenyl]}-2-hydroxysuccinic anhydride (2 step yield: 50%). [0246] 25 e) To a solution of 2-isopropyl-3-{4-[(hydroxymethyl)phenyl]}-2-hydroxysuccinic anhydride (224 mg, 0.742 mmol) in methanol (9 mL) was added sulfuric acid (18 mg, 0.18 mmol), and the mixture was heated under reflux with stirring for 12 h. The reaction mixture was 30 directly concentrated under reduced pressure, and the residue thus obtained was subjected to silica gel column chromatography (hexane - diethyl ether 1 : 1 ) to give 101 mg of the title compound as oil (yield: 52%). 'H NMR (CDCl 3 ) 5 1.28 (d, J = 7.0 Hz, 6H), 3.07 (septet, J = 35 7.0.Hz, 1H), 3.80 (s, 2H), 4.68 (d, J = 4.4 Hz, 2H), 7.22 (d, J = 8.1 Hz, 2H), 7.33 (d, J = 8.1 Hz, 2H).

[0247] Example 69: 2-(4-Carboxybenzyl)-3-isopropylmaleic acid dimethyl ester a) To a solution of 5 2-[4-(hydroxymethyl)benzyl]-3-isopropylmaleic anhydride (101 mg, 0.388 mmol) in a mixed solvent of methanol (1.0 mL) and tetrahydrofuran (1.0 mL) was added a 2.0 M trimethylsilyl diazomethane-hexane solution (1.0 mL, 2.0 mmol) with stirring under ice cooling. The reaction mixture was stirred at room 10 temperature for 2 h and directly concentrated under reduced pressure. The residue thus obtained was subjected to silica gel column chromatography (toluene-ethyl acetate 4 : 1 -+ 3 : 1) to give 96 mg of dimethyl 2-[4-(hydroxymethyl)benzyl]-3-isopropylmaleate as oil (yield: 15 81%). [0248] b) To a solution of dimethyl 2-[4-(hydroxymethyl)benzyl]-3-isopropylmaleate (96 mg, 0.31 mmol) in benzene (2.0 mL) was added active manganese 20 dioxide (602 mg), and the mixture was vigorously stirred at room temperature for 3 h. After the reaction mixture was filtered, the filtrate was concentrated to dryness to give 79 mg of 2-(4-formylbenzyl)-3-isopropylmaleic acid dimethyl ester as colorless oil (yield: 83%). 25 [0249] c) To a suspension of 2-(4-formylbenzyl)-3-isopropylmaleic acid dimethyl ester (79 mg, 0.259 mmol) in a mixed solvent of dioxane (3.0 mL) and water (3.0 mL) were added amide sulfate (38 mg, 0.39 mmol) and sodium chlorite (35 mg, 0.39 mmol) 30 with stirring under ice cooling, and the mixture was stirred for 30 min. The reaction mixture was directly concentrated, and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 1 : 2) to give 80 mg of the title compound as oil (yield: 96%). 35 'H NMR (CDCi 3 ) 6 1.12 (d, J = 6.8 Hz, 6H), 2.95 (septet, J = 6.8 Hz, 1H), 3.66 (s, 3H), 3.83 (s, 5H), 7.28 (m, 2H), 8.03 (m, 2H); MS (ESI+) m/z 321 [(M+H)*]. [0250] Example 70: 2-(4-Carboxybenzyl)-3-isopropylmaleic acid trisodium 5 In the same manner as Example 2, the title compound was obtained from 2-(4-carboxybenzyl)-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 1H NMR (D20) 6 0.89 (d, J = 6.8 Hz, 6H), 2.67 (septet, J = 6.8 Hz, 1H), 3.55 (s, 2H), 7.18 (d, 2H), 7.61 (d, 2H); MS (FAB+) 10 m/z 359 [(M+H)*]. [0251] Example 71: 2-(4-Carbamoylbenzyl)-3-isopropylmaleic acid dimethyl ester To a solution of 2-(4-carboxybenzyl)-3-isopropylmaleic acid 15 dimethyl ester (7.8 mg, 0.024 mmol) in tetrahydrofuran (400 pL) were added a 0.5 M ammonia-dioxane solution (600 pL) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (14.8 mg, 0.0535 mmol), and the mixture was stirred at room temperature for 4 h. The reaction mixture was directly 20 concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 1 : 4) to give 7.0 mg of the title compound as a solid (yield: 90%). 'H NMR (CDCI 3 ) 6 1.11 (d, J = 6.8 Hz, 6H), 2.95 (septet, J = 25 6.8 Hz, 1H), 3.65 (s, 3H), 3.80 (s, 2H), 3.82 (s, 3H), 5.54 (br s, 1H), 6.02 (br s, 1H), 7.26 (d, J = 8.5 Hz, 2H), 7.74 (d, J = 8.5 Hz, 2H); MS (ESI+) m/z 320 [(M+H)+]. [0252] Example 72: 30 2-(4-Carbamoylbenzyl)-3-isopropylmaleic acid disodium In the same manner as Example 2, the title compound was obtained from 2-(4-carbamoylbenzyl)-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.87 (d, J = 6.8 Hz, 6H), 2.66 (septet, J = 6.8 35 Hz, 1H), 3.57 (s, 2H), 7.23 (d, J = 8.2 Hz, 2H), 7.56 (d, J = 8.2 Hz, 2H); MS (FAB+) m/z 336 [(M+H)].

[0253] Example 73: 2-Isopropyl-3-[4-(morpholine-1-carbonyl)benzyl]-maleica acid dimethyl ester 5 To a solution of 2-(4-carboxybenzyl)-3-isopropylmaleic acid dimethyl ester (9.7 mg, 0.030 mmol) in dichloromethane (300 pL) were added 1-hydroxybenzotriazole (14.1 mg, 0.104 mmol), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (18.8 mg, 0.0981 mol) and morpholine (12.9 mg, 0.151 mmol) 10 with stirring under ice cooling. After stirring under ice cooling for 2 h and at room temperature for further 1 h, The reaction mixture was diluted with water and chloroform to separate the phases. The aqueous layer was extracted again with ethyl acetate, and the combined organic layer was concentrated 15 under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (ethyl acetate alone) to give 11.8 mg of the title compound as oil (quantitative). 'H NMR (CDCl 3 ) 6 1.12 (d, J = 6.8 Hz, 6H), 2.95 (septet, J = 20 6.8 Hz, 1H), 3.35-3.82, (m, 8H), 3.66 (s, 3H), 3.78 (s, 2H), 3.82 (s, 3H), 7.22 (m, 2H), 7.34 (m, 2H). [0254] Example 74: 2-Iisopropyl-3-[4-(morpholine-1-carbonyl)benzyl]maleic acid 25 disodium In the same manner as Example 2, the title compound was obtained from 2-isopropyl-3-[4-(morpholine-1-carbonyl)benzyl]maleic acid dimethyl ester as a solid (quantitative). 30 'H NMR (D 2 0) 6 0.86 (d, J = 6.8 Hz, 6H), 2.65 (septet, J = 6.8 Hz, 1H), 3.34 (m, 2H), 3.50 (m, 2H), 3.53 (s, 2H), 3.57 (m, 2H), 3.64 (m, 2H), 7.15-7.20 (m, 4H); MS (ESI+) m/z 362 {[(M-2Na+2H)+H]+}. [0255] 35 Example 75: 2-Iisopropyl-3-[4-(piperazine-1-carbonyl)benzyl]imaleica acid dimethyl ester hydrochloride a) In the same manner as Example 73, 2-{4-[4-(t-butoxycarbonyl)piperazine-1-carbonyl]benzyl}-3-iso propylmaleic acid dimethyl ester was obtained from 5 2-(4-carboxybenzyl)-3-isopropylmaleic acid dimethyl ester and t-butyl piperazine-1-carboxylate (quantitative). [0256] b) To a solution of 2-{4-[4-(t-butoxycarbonyl)piperazine-1-carbonyl]benzyl}-3-iso 10 propylmaleic acid dimethyl ester (11.8 mg, 0.0302 mmol) in methanol (200 pL) was added 5 M hydrochloric acid (200 pL) at room temperature, and the mixture was left standing for 5 h. The reaction mixture was directly concentrated to dryness to give 10.2 mg of the title compound as a solid (quantitative). 15 'H NMR (CDCl 3 ) 6 0.89 (d, J = 7.0 Hz, 6H), 2.92 (septet, J = 7.0 Hz, 1H), 3.09 (br s, 2H), 3.16 (br s, 2H), 3.49 (s, 3H), 3.58 (br s, 2H), 3.67 (s, 3H), 3.70 (s, 2H), 3.81 (br s, 2H), 7.16 (d, J = 8.1 Hz, 2H), 7.24 (d, J = 8.1 Hz, 2H). [0257] 20 Example 76: 2-Isopropyl-3-[4-(piperazine-1-carbonyl)benzyl]maleic acid disodium In the same manner as Example 2, the title compound was obtained from 25 2-isopropyl-3-[4-(piperazine-1-carbonyl)benzyl]maleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.87 (d, J = 7.0 Hz, 6H), 2.57-2.73 (m, 5H), 3.27 (m, 1H), 3.50-3.53 (m, 3H), 7.13-7.20 (m, 4H); MS (ESI-) m/z 359 {[(M-2Na+2H)-H]~}. 30 [0258] Example 77: 2-[4-(4-Acetoxypiperidin-1-carbonyl)benzyl]-3-isopropylmaleic acid dimethyl ester To a solution of 2-(4-carboxybenzyl)-3-isopropylmaleic acid 35 dimethyl ester (10.3 mg, 0.0322 mmol) in dichloromethane (500 pL) were added 1-hydroxybenzotriazole (13.3 mg, 0.0965 mmol), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (20.8 mg, 0.109 mol), piperidin-4-yl acetate hydrochloride (28.8 mg, 0.161 mmol) and triethylamine (14.6 mg, 0.145 mmol) with stirring under ice cooling. After stirring at 5 room temperature for 1 h, the reaction mixture was diluted with water to separate the phases. The aqueous layer was extracted again with ethyl acetate, and the combined organic layer was concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography 10 (hexane - ethyl acetatel:2) to give 11.1 mg of the title compound as a solid (yield: 78%). 1H NMR (CDCI 3 ) 6 1.11 (d, J = 7.0 Hz, 6H), 1.61 (br s, 1H), 1.74 (br s, 1H), 1.86 (br s, 1H), 1.96 (br s, 1H), 2.08 (br s, 3H), 2.96 (septet, J = 7.0 Hz, 1H), 3.31 (br s, 1H), 3.53 (br s, 15 1H), 3.66 (br s, 1H), 3.66 (s, 3H), 3.78 (s, 2H), 3.82 (s, 3H), 4.07 (br s, 1H), 5.01 (m, 1H), 7.21 (m, 2H), 7.33 (m, 2H). [0259] Example 78: 2-[4-(4-Hydroxypiperidin-1-carbonyl)benzyl]-3-isopropylmaleic 20 acid disodium In the same manner as Example 2, the title compound was obtained from 2-[4-(4-acetoxypiperidin-1-carbonyl)benzyl]-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 25 'H NMR (D20) 6 0.89 (d, J = 7.0 Hz, 6H), 1.28-1.44 (m, 2H), 1.65-1.91 (m, 2H), 2.69 (septet, I = 7.0 Hz, 1H), 3.04-3.12 (m, 2H). 3.55 (m, 1H), 3.56 (s, 2H), 3.82 (m, 1H), 4.04 (m, 1H), 7.15-7.23 (m, 4H); MS (ESI+) m/z 376 {[(M-2Na+2H)+H]*}. [0260] 30 Example 79: 2- [4-(2-Amno-2-oxoethylcarbamoyl)benzyl]-3-isopropylmaleic acid dimethyl estere In the same manner as Example 77, the title compound was obtained from 2-(4-carboxybenzyl)-3-isopropylmaleic acid 35 dimethyl ester and glycineamide hydrochloride as a solid (yield: 84%).

'H NMR (CD 3 0D) 6 1.08 (d, J = 6.8 Hz, 6H), 3.03 (septet, J = 6.8 Hz, 1H), 3.62 (s, 3H), 3.76 (s, 3H), 3.84 (s, 2H), 4.02 (s, 2H), 7.28 (d, J = 8.3 Hz, 2H), 7.81 (d, J = 8.3 Hz, 2H). [0261] 5 Example 80: 2-[4-(2-Amino-2-oxoethylcarbamoyl)benzyl]-3-isopropylmaleic acid disodiume In the same manner as Example 2, the title compound was obtained from 10 2-[4-(2-amino-2-oxoethylcarbamoyl)benzyl]-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.87 (d, J = 6.8 Hz, 6H), 2.65 (septet, J = 6.8 Hz, 1H), 3.57 (s, 2H), 3.92 (s, 2H), 7.24 (d, J = 8.3 Hz, 2H), 7.58 (d, J = 8.3 Hz, 2H); MS (ESI+) m/z 349 15 {[(M-2Na+2H)+H]*}. [0262] Example 81 (Synthesis Example): 2-Isopropyl-3-(4-methoxybenzyl)maleic anhydride In the same manner as Example 50, the title compound was 20 obtained from benzyl 3-methyl-2-ketobutyrate and benzyl 3-(4-methoxyphenyl)propionate as oil (yield: 51%). 'H NMR (CDCl3) 6 1.28 (d, J = 7.0 Hz, 6H), 3.05 (septet, J = 7.0 Hz, 1H), 3.74 (s, 2H), 3.79 (s, 3H), 6.85 (d, J = 8.7 Hz, 2H), 7.14 (d, J = 8.7 Hz, 2H). 25 [0263] Example 82: 2-Isopropyl-3-(4-methoxybenzyl)maleic acid disodium In the same manner as Example 11, the title compound was obtained from 2-isopropyl-3-(4-methoxybenzyl)maleic 30 anhydride as a solid (quantitative). 1H NMR (D 2 0) 6 0.85 (d, J = 7.0 Hz, 6H), 2.65 (septet, J = 7.0 Hz, 1H), 3.41 (s, 2H), 3.61 (s, 3H), 6.74 (m, 2H), 7.04 (m, 2H); MS (ESI+) m/z 323 [(M+H)*]. [0264] 35 Example 83 (Synthesis Example): Benzyl 3-[4-(t-butydimethylsilyloxy)phenyl]propionate a) To a solution of 3-(4-hydroxyphenyl)propionic acid (1.49 g, 8.96 mmol) in dimethylformamide (21.5 mL) were added potassium carbonate (1.30 g, 9.41 mmol) and benzyl bromide (1.69 g, 9.86 mmol), and the mixture was stirred at room 5 temperature for 12 h. The reaction mixture was diluted with toluene and water to separate the phases. The residue obtained by concentrating the organic layer under reduced pressure was subjected to silica gel column chromatography (hexane - ethyl acetate 5 : 1 -+ 3 : 1) to give 2.16 g of benzyl 10 3-(4-hydroxyphenyl)propionate as oil (yield: 94%). [0265] b) In the same manner as Example 12, the title compound was obtained from benzyl 3-(4-hydroxyphenyl)propionate as oil (yield: 93%). 15 'H NMR (CDCl 3 ) 6 0.18 (s, 6H), 0.98 (s, 9H), 2.65 (m, 2H), 2.90 (m, 2H), 5.11 (s, 2H), 6.73 (m, 2H), 7.03 (m, 2H), 7.26-7.40 (m, 5H). [0266] Example 84 (Synthesis Example): 20 2-[4-(t-Butyldimethylsilyloxy)benzyl]-3-isopropylmaleic anhydride In the same manner as Example 50, the title compound was obtained from benzyl 3-methyl-2-ketobutyrate and benzyl 3-[4-(t-butyldimethylsilyloxy)phenyl]propionate as oil (yield: 25 58%). 1 H NMR (CDC13) 6 0.18 (s, 6H), 0.97 (s, 9H), 1.25 (d, J = 6.8 Hz, 6H), 3.02 (septet, J = 6.8 Hz, 1H), 3.72 (s, 2H), 6.78 (m, 2H), 7.06 (m, 2H). [0267] 30 Example 85: 2-(4-Hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester a) To a solution of 2-[4-(t-butyldimethylsilyloxy)benzyl]-3-isopropylmaleic anhydride (823 mg, 2.28 mmol) in methanol (15 mL) was added 35 a 2.0 M trimethylsilyl diazomethane-hexane solution (8.5 mL, 17 mmol) with stirring at room temperature. After stirring at room temperature for 30 min, the reaction mixture was concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate 10 : 1 -+ 8 : 1) to give 913 mg of 5 dimethyl 2-[4-(t-butyldimethylsilyloxy)benzyl]-3-isopropylmaleate as oil (yield: 98%). [0268] b) To a solution of dimethyl 10 2-[4-(t-butyldimethylsilyloxy)benzyl]-3-isopropylmaleate (823 mg, 2.02 mmol) in tetrahydrofuran (13 mL) was added a 1 M tetrabutylammonium fluoride-tetrahydrofuran solution (2.1 mL, 2.1 mmol) at room temperature, and the mixture was left standing at room temperature for 1 h. The reaction mixture was 15 directly concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (hexane - ethyl acetate2:1) to give 592 mg of 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 20 'H NMR (CDCl 3 ) 6 1.11 (d, J = 6.8 Hz, 6H), 2.98 (septet, J = 6.8 Hz, 1H), 3.65 (s, 3H), 3.68 (s, 2H), 3.80 (s, 3H), 4.98 (br s, 1H), 6.74 (m, 2H), 7.02 (m, 2H). [0269] Example 86: 25 2-(4-Oxidobenzyl)-3-isopropylmaleic acid trisodium In the same manner as Example 2, the title compound was obtained from 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.90 (d, J = 7.0 Hz, 6H), 2.71 (septet, J = 7.0 30 Hz, 1H), 3.36 (s, 2H), 6.45 (m, J = 8.5 Hz, 2H), 6.90 (d, J = 8.5 Hz, 2H); MS (FAB+) m/z 331 [(M+H)+]. [0270] Example 87: 2-Isopropyl-3-[4-(2-methoxy-2-oxoethoxy)benzyl]maleic acid 35 dimethyl ester To a solution of 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester (44.1 mg, 0.151 mmol) in (880 pL) were added potassium carbonate (34.7 mg, 0.251 mmol) and methyl bromoacetate (138.4 mg, 0.904 mmol) with stirring at room temperature. After stirring at room temperature for 20 h, the 5 reaction mixture was diluted with toluene and water to separate the phases. The aqueous layer was extracted again with toluene, and the combined organic layer was concentrated under reduced pressure and the residue thus obtained was subjected to silica gel column chromatography (toluene-ethyl 10 acetate 10 : 1) to give 45.2 mg of the title compound as oil (yield: 82%). 1 H NMR (CDC 3 ) 6 1.11 (d, J = 7.0 Hz, 6H), 2.97 (septet, J = 7.0 Hz, 1H), 3.65 (s, 3H), 3.69 (s, 2H), 3.80 (s, 3H), 3.81 (s, 3H), 4.61 (s, 2H), 6.83 (m, 2H), 7.09 (m, 2H). 15 [0271] Example 88: 2-[4-(Carboxylatomethoxy)benzyl]-3-isopropylmaleic acid tridodium In the same manner as Example 2, the title compound was 20 obtained from 2-isopropyl-3-[4-(2-methoxy-2-oxoethoxy)benzyl]maleic acid dimethyl ester as a solid (quantitative). 1 H NMR (D 2 0) 6 0.89 (d, J = 7.0 Hz, 6H), 2.68 (septet, J = 7.0 Hz, 1H), 3.45 (s, 2H), 4.29 (s, 2H), 6.72 (d, J = 8.5 Hz, 2H), 25 7.06 (d, J = 8.5 Hz, 2H); MS (FAB+) m/z 389 [(M+H)*]. [0272] Example 89: 2-[4-(2-Amino-2-oxoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester 30 In the same manner as Example 87, the title compound was obtained from 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester and bromoacetamide as oil (yield: 80%). 'H NMR (CDCl 3 ) 6 1.12 (d, J = 7.0 Hz, 6H), 2.98 (septet, J = 7.0 Hz, 1H), 3.65 (s, 3H), 3.67 (s, 2H), 3.80 (s, 3H), 4.47 (s, 35 2H), 5.58 (br s, 1H), 6.54 (br s, 1H), 6.84 (m, 2H), 7.12 (m, 2H); MS (FAB+) m/z 372 [(M+Na)+].

[0273] Example 90: 2-[4-(2-Amino-2-oxoethoxy)benzyl]-3-isopropylmaleic acid disodium 5 In the same manner as Example 2, the title compound was obtained from 2-[4-(2-amino-2-oxoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.91 (d, J = 6.8 Hz, 6H), 2.70 (septet, J = 6.8 10 Hz, 1H), 3.48 (s, 2H), 4.47 (s, 2H), 6.81 (d, J = 8.5 Hz, 2H), 7.11 (d, J = 8.5 Hz, 2H); MS (FAB+) m/z 320 {[(M-2Na+2H)+H]+}. [0274] Example 91: 15 2-[4-(2-Aminoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester a) To a solution of 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester (204.5 mg, 0.6996 mmol) in benzene (6 mL) were added N-(t-butoxycarbonyl)ethanolamine(343 mg, 2.13 20 mol), tributylphosphine (853 mg, 4.20 mmol) and 1,1'-(azodicarbonyl)dipiperidine (1.05 g, 4.20 mmol) with stirring at room temperature. After stirring at room temperature for 1 h, the reaction mixture was filtered, and the residue obtained by concentrating the filtrate under reduced pressure 25 was subjected to silica gel column chromatography (hexane ethyl acetate 2 : 1) to give 287 mg of dimethyl 2-{4-[2-(t-butoxycarbonylamino)ethoxy]benzyl}-3-isopropylmal eate as oil (yield: 98%). [0275] 30 b) To a solution of dimethyl 2-{4-[2-(t-butoxycarbonylamino)ethoxy]benzyl}-3-isopropylmal eate (287 mg, 0.659 mmol) in methanol (6 mL) was added 5 M hydrochloric acid (5.9 mL) at room temperature, and the mixture was left standing at room temperature for 20 h. The 35 residue obtained by concentrating the mixture under reduced pressure was dissolved in ethyl acetate, and a saturated aqueous sodium hydrogen carbonate solution was added. After separating the phases, the organic layer was concentrated and the residue thus obtained was subjected to LH-20 (dichloromethane - methanol 1 : 1 ) to give 222 mg of the title 5 compound as oil (quantitative). 'H NMR (CDCl 3 ) 6 1.11 (d, J = 7.0 Hz, 6H), 2.99 (septet, J = 7.0 Hz, 1H), 3.07 (t, J = 5.1 Hz, 2H), 3.65 (s, 3H), 3.69 (s, 2H), 3.80 (s, 3H), 3.96 (t, J = 5.1 Hz, 2H), 6.82 (m, 2H), 7.08 (m, 2H). 10 [0276] Example 92: 2-[4-(2-aminoethoxy)benzyl]-3-isopropylmaleic acid disodium In the same manner as Example 2, the title compound was obtained from 2-[4-(2-aminoethoxy)benzyl]-3-isopropylmaleic 15 acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.89 (d, J = 7.0 Hz, 6H), 2.68 (septet, J = 7.0 Hz, 1H), 2.82 (m, 2H), 3.45 (s, 2H), 3.91 (m, 2H), 6.79 (m, 2H), 7.07 (m, 2H); MS (FAB+) m/z 352 [(M+H)*]. [0277] 20 Example 93: 2-{4-[2-(1H-Imidazol-1-yl)ethoxy]benzyl}-3-isopropylmaleic acid dimethyl ester In the same manner as Example 91a, the title compound was obtained from 2-(4-hydroxybenzyl)-3-isopropylmaleic acid 25 dimethyl ester and 1-(2-hydroxyethyl)imidazole as oil (yield: 52%). 'H NMR (CDCI 3 ) 6 1.11 (d, J = 7.0 Hz, 6H), 2.97 (septet, J = 7.0 Hz, 1H), 3.01 (s, 3H), 3.68 (s, 2H), 3.80 (s, 3H), 4.18 (t, J = 5.0 Hz, 2H), 4.32 (t, J = 5.0 Hz, 2H), 6.78 (m, 2H), 30 7.03-7.09 (m, 4H), 7.60 (m, 1H); MS (ESI+) m/z 387 [(M+H)+]. [0278] Example 94: 2-{4-[2-(1H-Imidazol-1-yl)ethoxy]benzyl}-3-isopropylmaleic 35 acid disodium In the same manner as Example 2, the title compound was obtained from 2-{4-[2-(1H-imidazol-1-yl)ethoxy] benzyl}-3-isopropylmaleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.88 (d, J = 7.0 Hz, 6H), 2.67 (septet, ) = 7.0 5 Hz, 1H), 3.45 (s, 2H), 4.20 (m, 2H), 4.24 (m, 2H), 6.72 (m, 2H), 6.85 (m, 1H), 7.03-7.08 (m, 3H), 7.58 (s, 1H); MS (ESI+) m/z 403 [(M+H)*]. [0279] Example 95: 10 2 -Isopropyl-3-[4-(pyrrolidine-3-yloxy)benzyl]maleic acid dimethyl ester In the same manner as Example 91, the title compound was obtained from 2-(4-hydroxybenzyl)-3-isopropylmaleic acid dimethyl ester and N-(t-butoxycarbonyl)-3-hydroxypyrrolidine 15 as oil (yield: 82%). 1H NMR (CDCl 3 ) 6 1.11 (d, J = 7.0 Hz, 6H), 1.93 (m, 1H), 2.07 (m, 1H), 2.84-3.02 (m, 3H), 3.15-3.20 (m, 2H), 3.65 (s, 3H), 3.69 (s, 2H), 3.80 (s, 3H), 4.79 (m, 1H), 6.77 (m, 2H), 7.06 (m, 2H). 20 [0280] Example 96: 2-isopropyl-3-[4-(pyrrolidine-3-yloxy)benzyl]maleic acid disodium In the same manner as Example 2, the title compound was 25 obtained from 2-isopropyl-3-[4-(pyrrolidine-3-yloxy)benzyl]maleic acid dimethyl ester as a solid (quantitative). 'H NMR (D 2 0) 6 0.90 (d, J = 7.0 Hz, 6H), 1.84-1.98 (m, 2H), 2.69 (septet, J = 7.0 Hz, 1H), 2.70-3.05 (m, 4H), 3.45 (s, 2H), 30 4.85 (m, 1H), 6.78 (m, 2H), 7.08 (m, 2H); MS (ESI+) m/z 334 {[(M-2Na+2H)+H]*}. [0281] Example 97: 2-[4-(2-Guanidinoethoxy)benzyl]-3-isopropylmaleic acid 35 dimethyl ester hydrochloride a) To a solution of 2-[4-(2-aminoethoxy)benzyl]-3-isopropylmaleic acid dimethyl ester (30.2 mg, 0.0900 mmol) in tetrahydrofuran (900 pL) was added N,N'-bis(t-butoxycarbonyl)-1-guanylpyrazole (35.2 mg, 0.113 mmol) at room temperature, and the mixture was left 5 standing for 20 h. The reaction mixture was directly concentrated under reduced pressure, and the residue obtained was subjected to silica gel column chromatography (hexane ethyl acetate 3 : 1) to give 47.6 mg of dimethyl 2-[4-{2-[2,3-bis(t-butoxycarbonyl)guanidino]ethoxylbenzyl]-3 10 isopropylmaleate as a syrup (yield: 92%). [0282] b) To a solution of dimethyl 2-[4-{2-[2,3-bis(t-butoxycarbonyl)guanidino]ethoxylbenzyl]-3 isopropylmaleate (23.5 mg, 0.0407 mmol) in methanol (470 pL) 15 was added 5 M hydrochloric acid (407 pL) at room temperature, and the mixture was left standing for 4 h. The reaction mixture was concentrated to dryness to give 17.6 mg of the title compound as oil (quantitative). 1H NMR (D 2 0) 6 0.91 (d, J = 7.0 Hz, 6H), 2.94 (septet, J = 7.0 20 Hz, 1H), 3.43 (t, J = 5.0 Hz, 3H), 3.50 (s, 3H), 3.57 (s, 2H), 3.68 (s, 3H), 4.02 (t, J = 5.0 Hz, 2H), 6.80 (m, 2H), 7.01 (m, 2H). [0283] Example 98: 25 2-[4-(2-guanidinoethoxy)benzyl]-3-isopropylmaleic acid disodiurn In the same manner as Example 2, the title compound was obtained from 2-[4-(2-guanidinoethoxy)benzyl]-3-isopropylmaleic acid 30 dimethyl ester hydrochloride as a solid (quantitative). 1H NMR (D 2 0) 6 0.92 (d, J = 6.8 Hz, 6H), 2.97 (m, 1H), 2.79 (septet, J = 6.8 Hz, 1H), 2.79 (m, 2H), 3.69 (s, 2H), 4.04 (m, 2H), 6.83 (m, 2H), 7.07 (m, 2H); MS (ESI+) m/z 350 {[(M-2Na+2H)+H]+}. 35 [0284] The compounds prepared above have the structures as shown in the following Tables. Table 1 - 1 Example Compounds Example Compounds 1 12 TBS MeO2C CO2Me CO 2 Et 2 13 NaO 2 C C0 2 Na 0 3 14 OH EtO 2 C CO 2 Et NaO 2 C CO 2 Na NaO 2 C CO 2 Na O15 0 0 5 16

KO

2 C CO 2 K NaO 2 C CO 2 Na 6 17 EtO 2 C CO 2 Et 7 18 NaO 2 C CO 2 Na NaO 2 C CO 2 Na 8 19 MeO 2 C CO 2 Me EtO 2 C CO 2 Et 9 20 NaO 2 C CO 2 Na NaO 2 C CO 2 Na 10 21 00O-O 000 11 22 NaO 2 C CO 2 Na NaO 2 C CO 2 Na Table 1 -2 Example Compounds Example Compounds 23 34 0 0 NaO 2 C CO 2 Na 24 35 NaO 2 C CO 2 Na BnO 2 C 25 36 000t 0 0 0 26 37 NaO 2 C CO 2 Na NaO 2 C CO 2 Na 27 038 28 39 NaO 2 C CO 2 Na NaO 2 C CO 2 Na 29 40 0~0 0 30 41 NaO 2 C C 2 Na2Na 31 42 0 00 00 0 32 43 NaO 2 C CO 2 Na NaO 2 C CO 2 Na OTBS TBS 33 44 0CO0B0

CO

2 Bn Table 1 - 3 Example Compounds Example Compounds PH 45 56 No NaO 2 C CO 2 Na 00 OH 46 57 NOjOH MeO 2 C CO 2 Me NaO 2 C CO 2 Na OH 47 58 Na OH NaO 2 C CO 2 Na 000 OH OH 48 59 NaO 2 C CO 2 Na 49 60 OH NaO 2 C CO 2 Na

CO

2 Bn 0 50 61 00 51 62 NaO 2 C CO 2 Na NaO 2 C CO 2 Na 52 - 63 CO 2 Bn 53 N 64 Na2 Na 54 - N 65 NaO 2 C CO 2 Na 0 O O 5 5 N N aONO 6 6 MeO 2 C CO 2 Me NaO 2 C CO 2 Na Table 1 - 4 Example Compounds Example Compounds OTBDPS 67 78 NaO 2 C CO 2 Na

CO

2 Et O OH - OH- 0 68 H 79 -Me NH 69 OH 80 NaO H NH2 Na2 CO2Na0Na -H MeO2C CO2Me 0 76 H2 80 NaO 2 C CO 2 Na 0 70 M COM 812O /O NaO 2 C CO 2 Na 0e2 O2Me 75 - HC 826 \O~a MeO 2 C C 2 Me NaO 2 C CO 2 Na 72 -NH 2 83 \O B NaO 2 C CO 2 Na

CO

2 Bn

-

0/ P1,O B 73

-

84 ~/OB Me0 2 C CO 2 Me 0i & 74 - t~ N-\ 85 - \/aOH NaO 2 C CO 2 Na 00MeO 2 C C0 2 Me -0 OONa 75 N *HCI 86 r- \O - 0 0 - N\ 0 ONa 77 MeO 2 C CO 2 Me 88 NaO 2 C CO 2 Na

CH

3 Table 1 - 5 Example Compounds Example Compounds 89 1O

NH

2 9 MeO 2 C CO 2 Me NaO 2 C CO 2 Na 90 - \ O NH 2 95 5 NaO 2 C CO 2 Na MeO 2 C CO 2 Me 0H 0 NH 2 MeO 2 C CO 2 MeNa2 CON Na02 C02aaM0 2 C CO 2 Na

NH

2

NH

2 N 92 - NH NaO 2 C CO 2 Na MeO 2 C CO 2 Me n HC

NH

2 /-j r- H NH 93 798 N MeO 2 C CO 2 Me NaO 2 C CO 2 Na [0285] Inhibitory activity The total length of IMP-1 as a metallo-1-lactamase was amplified by PCR with a template of blaIMP-1 of Pseudomonas 5 aeruginosa MSC15369 which possesses IMP-1. The PCR product was integrated into pTrcHis2 TOPO vector (Invitrogen), and then introduced into E. coli DH5a (TOYOBO) for culture under the induction of 0.5 mM Isopropyl-@-D-(-)-thiogalactopyranoside (Wako) at 37 *C for 3 h 10 to express IMP-1. After recovering the bacterial cells, the periplasmic fraction was extracted by ocmotic shock with sucrose to refine the IMP-1 by Ni-NTA Slurry (QIAGEN) with the His tag at the C-terminus. Similarly, blavM-2 gene of Pseudomonas putida MSC06534 which possesses VIM-2 as a 15 metallo-P-lactamase was integrated and expressed in E. coli DH5a to refine the VIM-2. [0286] In order to determine the inhibitory activity of metallo-P-lactamase, a 50 mM HEPES (pH 7.5) buffer (referred 20 to hereinafter as "buffer") was used, and nitrocefin (Oxoid) with the final concentration of 100 pM was used as a substrate. A test drug (material to be tested: compound having an Example Number shown in Table 2) was added to each well of a 96-well plate, into which nitrocefin was added and mixed. Then, IMP-1 25 or VIM-2 was added to each well in the final concentration of 1 nM or 1.5 nM, respectively to react the mixture at room temperature for 20 min. In this case, ZnSO 4 was added in the final concentration of 100 pM in order to exclude the inhibitory effect by the chelate effect. Enzyme inhibiting activity was 30 determined by measuring the hydrolysis activity of nitrocefin at a wave length of 490 nm with ARVOsx microplate reader (Wallac). A reaction solution without metallo-01-lactamase was prepared as a control, and the concentration of the test drug which exhibits 50% inhibition was set as IC 5 o. The results are 35 shown in Table 2.

[0287] (Inhibitory activity) Table 2 5

IC

5 , of each inhibitor against IMP-1 and VIM-2 Example Compounds IMP-1 (A M) VIM-2 (y M) 4 2.5 13 NaO 2 C CO 2 Na 11 0.29 3.0 NaO 2 C CO 2 Na 22 2.4 7.3 NaO 2 C CO 2 Na 43 0. 19 0. 16 NaO 2 C CO 2 Na 51 1.9 1.8 NaO 2 C CO 2 Na 54 2.8 5.3 NaO 2 C CO 2 Na 66 1.2 14 NaO 2 C CO 2 Na 72

NH

2 1.4 0.60 NaO 2 C CO 2 Na 86 Na 0.56 1.9 NaO 2 C CO 2 Na

NH

2 92 0 0.98 0.30 NaO 2 C CO 2 Na [0288] Combination effect IMP-1 producing Pseudomonas aeruginosa strain PAO/pMS363 10 (FEMS Microbiology Letters 1994, 121, 175) was used for evaluating the inhibitory effect of maleic acid derivatives on the resistance against carbapenem by the metallo-1-lactamase in bacteria. Minimum inhibitory concentration (MIC) of imipenem, meropenem, biapenem, doripenem, CS-023, ceftriaxone, ceftadizime, and cefepime against IMP-1 producing Pseudomonas aeruginosa was measured by the microliquid dilution method which is the standard method of Japanese 5 Society of Chemotherapy (Chemotherapy 1981, 29, 76). That is, a strain which had been cultured overnight in a Mueller-Hinton broth was adjusted to a concentration of 104 CFU/well, and added to the same media containing respective concentrations of imipenem (IPM), meropenem (MEPM), biapenem (BIPM), 10 doripenem (DRPM) and CS-023. The compound of the present invention was added to each well in the final concentration of 50 pg/ml to confirm its effect on the basis of MIC of imipenem, meropenem, biapenem, doripenem, and CS-023. The results are shown in Table 3. 15 [0289] (Combination effect) 109 Table 3 Combination effect of the compounds with a variety of p-lact am drugs against metallo-1-lactamase producing Pseudomona 5 s aeruginosa (combination with 50 pg/ml of inhibitor) MIC of I -lactam drugs Example Compounds (combination with 50 pg/ml of inhibitor) IPM MEPM BIPM DRPM CS-023 4 2 4 1 4 4-8 NaO 2 C CO 2 Na 11 4 NT 4 NT NT NaO 2 C CO 2 Na 22 1-2 4 1 4 4 NaO 2 C CO 2 Na 43 . 2-4 16 2 16 16 NaO 2 C CO 2 Na 51 4 16 2 32 32 NaO 2 C CO 2 Na 54 16 32 4 32 32 NaO 2 C CO 2 Na 66 s 1 8 1 8 16 NaO 2 C CO 2 Na 72

H

2 8 16 8 16 64 NaO 2 C CO 2 Na 86 \/N a 4 8 1 16 32 NaO 2 C CO 2 Na

NH

2 92 0 2 4 0.5 4 8 NaO 2 C CO 2 Na -lactam 128-2 128 64-128 128 256 alone 56 110 [0290l It has been found that enzyme inhibiting As shown In Table 2, eIhibitors are observed in activities as the metallo-p-lactamase inhibitora (b),rthe maleic acid derivatives of the general formulate the 5 compounds of the present invention, which Inhibit both enzymes of IMP and VIM series. While few metalop-lactamase Inhibitors which exhibit the combination effect with p-lactam drugs have been described, it has been found that the compounds of the present invention, as shown In Table 3, have the effect of 10 recovering the activities of imipenemn by combininlg with Imipenern against the metallo-P-lactamase producing Pseudomonas aeruginosa of which therapy has hitherto been regarded difficult in the medical field. Thus, the compound represented by the general formula (1) is useful as the 15 metaoePlactayase Inhibitor for the combination with P-lactan drugs and the like. [0291] Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. [02921 The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates.

Claims (22)

1. A method of treating or preventing a bacterial infection comprising administering to a subject in need thereof a metallo-@-lactamase inhibitor, 5 which comprises a compound represented by the following formula (I), or a salt or solvate thereof: R' F M'OOC COOM' in which R' represents a C 2 - 6 alkyl group, a C 3 .7 cycloalkyl group, a hydroxymethyl 10 group, a -C 1 3 alkylene-phenyl group, a -Co-, alkylene-heterocycle, a -O-C- 6 alkyl group, or a -S-C 1 . 6 alkyl group, all of which may be substituted, R 2 represents a C 1 - 6 alkyl group, a C 3 -7 cycloalkyl group, a hydroxymethyl group, -C 1 3 alkylene-phenyl group, a -Co-, alkylene-heterocycle, a -O-CI alkyl group, or a -S-C 1 . 6 alkyl group, all of which may be substituted, 15 each M 1 independently represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which can be hydrolyzed in vivo.

2. A method according to Claim 1, wherein 20 R1 represents a C 2 - 6 alkyl group, a C3- 7 cycloalkyl group, a hydroxymethyl group, a benzyl group, phenethyl group, a -CO- alkylene-heterocycle, wherein the heterocycle represents tetrahydropyran, pyridine, or piperidine, a -0-C- 6 alkyl group, or a -S-C 1 - 6 alkyl group, all of which may be substituted, 25 R 2 represents a C 1 6 alkyl group, a C3- 7 cycloalkyl group, a hydroxymethyl group, a benzyl group, phenethyl group, a -CO- alkylene-heterocycle, wherein the heterocycle represents tetrahydropyran, pyridine, or piperidine, a -O-CI- ,alkyl group, or a -S-C 1 6 alkyl group, all of which may be H.AMM3F1-1l DN OCflIN KiO Il MAUL t UI(IS 193-195 LOC-23/0)4UOI 14I 112 substituted, the two M', which may be the same or different, represents a hydrogen atom, a pharmaceutically acceptable cation, or a pharmaceutically acceptable group which can be hydrolyzed in vivo. 5

3. A method according to Claim 1, wherein R' represents a C 2 6 alkyl group, a C 3 -7 cycloalkyl group, wherein said cycle may be substituted with a 10 hydroxyl group, or fused with an aryl, a hydroxymethyl group, a -Cr 3 alkylene-phenyl group, wherein said phenyl group may be substituted by a hydroxyl group, 15 a C- 6 alkyl group, a hydroxymethyl group, a group -COOM, wherein M represents a hydrogen atom or a pharmaceutically acceptable cation, a group -CO-NR R , wherein R 2 and R , which may be the same or 20 different, represents a hydrogen atom or a C 16 alkyl group, wherein said alkyl group may be further substituted with an aminocarbonyl group, or R 2 1 and R , together with the nitrogen atom to which R 2 and R 2 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl 25 group, or a piperidyl group, and the heterocycle may be substituted by a hydroxyl group or a Cl 6 alkanoyloxy group, a group -O-R 2 1 wherein R 2 represents a C 1 -6 alkyl group, wherein the alkyl group represents -COOM, wherein M represents a hydrogen atom, a C 1 6 alkyl group or a pharmaceutically acceptable cation, an aminocarbonyl 30 group, an amino group, a guanidino group, or a five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, or a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms, H:\Irrl ierwI c 11\NRI'OrI I)It W \K (,I J 9 _ I, OOC-23/10412014 113 preferably pyrrolidine, a -CO- alkylene-heterocycle, wherein said heterocycle represents a five- or six-membered saturated or unsaturated heterocycle containing one nitrogen or oxygen atom, and may be substituted by a hydroxyl group, 5 a -O-CI- 6 alkyl group, or a -S-C 1 6 alkyl group, and R 2 represents a C1 6 alkyl group, a C 3 7 cycloalkyl group, wherein said cycle may be substituted with a 10 hydroxyl group or may be fused with an aryl, a hydroxymethyl group, a -C 1 - alkylene-phenyl group, a -C 1 3 alkylene-phenyl group, wherein said phenyl group may be substituted by a hydroxyl group, 15 a C 1 6 alkyl group, a hydroxymethyl group, a group -COOM, wherein M represents a hydrogen atom or a pharmaceutically acceptable cation, a group -CO-NR R wherein R 2 and R , which may be the same or 20 different, represents a hydrogen atom or a C 1 6 alkyl group, wherein said alkyl group may be further substituted with an aminocarbonyl group, or R 22 and R , together with the nitrogen atom to which R 2 and R 2 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl 25 group, or a piperidyl group, and the heterocycle may be substituted by a hydroxyl group or a C 1 .6 alkanoyloxy group, a group -O-R 24 wherein R 24 represents a C1-6 alkyl group, wherein the alkyl group represents -COOM, wherein M represents a hydrogen atom, a C1s alkyl group or a pharmaceutically acceptable cation, an aminocarbonyl 30 group, an amino group, a guanidino group, or a five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, or a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms, 114 preferably pyrrolidine, a -CO. 1 alkylene-heterocycle, wherein said heterocycle represents a five- or six-membered saturated or unsaturated heterocycle containing one nitrogen or oxygen atom, and may be substituted by a hydroxyl group, 5 a -O-C 1 6 alkyl group, or a -S-C 1 6 alkyl group.

4. A method according to Claim 1, wherein R1 represents 10 a C 2 - alkyl group; a C3 7 cycloalkyl group, wherein said cycle may be substituted by a hydroxyl group or may be fused with aryl; a hydroxymethyl group; a -C1 alkylene-phenyl group, in which the phenyl group may be substituted 15 with a hydroxyl group, a C1 6 alkyl group, a hydroxymethyl group, a group -COOM, wherein M represents a hydrogen atom or a 20 pharmaceutically acceptable cation, a group -CO-NR R , wherein R 2 and R , which may be the same or different, represent a hydrogen atom or a Coe alkyl group, preferably a C 1 4 alkyl group, more preferably a C 12 alkyl group, wherein the alkyl group may be further substituted by an aminocarbonyl group, or R 2 and R , together 25 with the nitrogen atom to which R 22 and R 23 are bonded, may form a five- or six-membered saturated heterocyclic ring comprising 1-2 oxygen or nitrogen atoms, preferably, a morphonyl group, a piperazyl group, or a piperidyl group, and the heterocycle may be substituted by a hydroxyl group or a C 16 alkanoyloxy group, 30 a group -O-R 24 wherein R 24 represents a C1-6 alkyl group, wherein the alkyl group represents -COOM, wherein M represents a hydrogen atom, a CIe alkyl group or a pharmaceutically acceptable cation, an aminocarbonyl Ii:\rbr\lirwoe \ CNR'ortbl\DC\RB3R\6193495-1,doc-23/04/2014 115 group, an amino group, a guanidino group, or a five- or six-membered unsaturated heterocycle having 1-2 nitrogen atoms, or a five- or six-membered saturated heterocycle having 1-2 nitrogen atoms; a -CO- alkylene-heterocycle, in which the heterocycle represents five- or 5 six-membered saturated or unsaturated heterocycle comprising one nitrogen or oxygen atom, and may be substituted by a hydroxyl group; a -O-C- 6 alkyl group; or a -S-C 1 - alkyl group 10

5. A method according to Claim 1, wherein R' and R 2 represents a C 2 4 alkyl group.

6. A pharmaceutical composition which comprises a compound represented by the general formula (I) defined in Claim 1 and a 15 pharmaceutically acceptable carrier, which is used as metallo-p-lactamase inhibitor.

7. A pharmaceutical composition according to Claim 6, which is administered concomitantly or sequentially with a p-lactam antibiotic. 20

8. A pharmaceutical composition according to Claim 7, which is administered concomitantly or sequentially with dehydropeptidase and/or p-lactamase inhibitors other than the compound of the general formula (I). 25

9. A pharmaceutical composition according to Claim 7, wherein said p-lactam antibiotic is a carbapenem antibiotic.

10. A pharmaceutical composition which comprises a metallo-p-lactamase inhibitor defined in Claim 1, a p-lactam antibiotic, and optionally a 30 pharmaceutically acceptable carrier.

11. A pharmaceutical composition according to any one of Claims 6 to 10, 116 which is used as an antibiotic,

12. A process for treating bacterial infections, which comprises co-administering a s-lactam antibiotic and a metallo-P-lactamase inhibitor 5 defined in Claim 1.

13. A process according to Claim 12, wherein said p-lactam antibiotic is carbapenem antibiotic, 10

14. A compound represented by the following general formula (II), or a salt or solvate: (R 4 M 2 00C COOM 2 15 in which R 3 represents a C 2 - 6 alkyl group, a C 3 .7 cycloalkyl group, or a hydroxymethyl group, all of which may be substituted R" represents a C 1 . 6 alkyl group or a C 3 -7 cycloalkyl group, all of which may be substituted, 20 each M2 independently represents a sodium cation or a potassium cation,

15. A compound represented by the following general formula (III), or a salt or solvate thereof: M00C COOM 3 25 117 in which R 5 represents an ethyl group, R 6 represents a C 1 3 linear alkyl group, the two groups M 3 , represent a sodium cation or a potassium cation. 5

16. A compound represented by the following general formula (IV), or a salt or solvate thereof: (IV) M 4 00C COOM 4 in which 10 R 7 represents a C1s alkyl group, a C 3 -7 cycloalkyl group, a -C1 3 alkylene phenyl group, a -C, alkylene-ring A, a -0-CI- 6 alkyl group, or a -S-C1 6 alkyl group, all of which may be substituted, R 8 represents a -C 1 3 alkylene-phenyl group, a -Co 1 alkylene-ring A, a -0 C 1 6 alkyl group, or a -S-C 1 s alkyl group, all of which may be substituted, 15 the ring A represents a five- to ten-membered mono- or bi-cyclic heterocyclic ring having 1-4 hetero atoms selected from nitrogen, oxygen and sulfur atoms, each M 4 independently represents a sodium cation or a potassium cation, provided that when R 7 is a C 1 6 alkyl group, R" excludes dihydrofuran. 20

17. A compound according to Claim 16, wherein the ring A represents tetrahydrofuran, furan, pyrrolidine, piperidine, pyrazolidine, imidazolidine, piperazine, morpholine, thiomorpholine, pyrrole, thiophene, oxazole, isoxazole, thiazole, isothiazole, imidazole, pyrazole, pyridine, pyridazine, 25 pyrimidine, pyrazine, triazole, tetrazole, thiadiazole, azetidine, thiazoline, quinuclidine, triazine, isobenzofuran, indole, indolizine, chromene, quinoline, isoquinoline, cinnoline, quinazoline, quinoxaline, phthalazine, purine, pteridine, or a salt or solvate thereof. 118

18. A compound represented by the following general formula (V), or a salt or solvate thereof: R 9 R1 0 M 5 00C COOM 5 5 in which R 9 represents a C 1 s alkyl group, a C 3 7 cycloalkyl group, a -C 1 3 alkylene phenyl group, a -CO- alkylene-ring B, a -0-C- 6 alkyl group, or, a -S-C 1 s alkyl group, all of which may be substituted, R1 0 represents a -C- 3 alkylene-phenyl group, a -Co 1 alkylene-ring B, a -O-C 10 6 alkyl group, or a -S-C 1 6 alkyl group, all of which may be substituted, ring B represents pyridine, piperidine, or tetrahydropyran, each M 5 independently represents a sodium cation or a potassium cation, provided that the case when R 9 and R' 0 represent pyridinium is excluded. 15

19. Use of a metallo-p-lactamase inhibitor defined in any one of Claims 1 to 5 or a compound defined in any one of claims 14 to 18, for preventing or treating a bacterial infection.

20. Use of a metallo-P-lactamase inhibitor defined in any one of Claims 1 20 to 5 or a compound defined in any one of Claims 14 to 18 in the manufacture of a medicament for the prevention or treatment of a bacterial infection.

21. A method of treating or preventing a bacterial infection comprising 25 administering to a subject in need thereof a compound defined in any one of Claims 14 to 18,

22. A method according to Claim 1; a pharmaceutical composition 119 according to any one of Claims 6, 10 and 11; a process according to Claim 12; a compound according to any one of Claims 14 to 16 and 18; a use according to Claim 19 or 20; or a method according to Claim 21; substantially as hereinbefore described and/or exemplified.