AU2002366191B2 - Gabusectin derivatives, method for the production thereof and use of the same - Google Patents

Abstract

3-(Octahydronaphth-1-yl-methylene)-pyrrolidine derivatives (I) and its derivatives, are new. Gabusectin compounds of formula (I) and their stereoisomers and tautomers (including mixtures in all ratios) and salts are new. R1-R3 = H; or 1-6C alkyl, 2-6C alkenyl or 2-6C alkynyl (all optionally substituted by 1-2 of OH, =O, halo or NH2; or 1-6C alkoxy, 2-6C alkenyloxy, aryl, 1-6C alkylamino or 2-6C alkenylamino (all optionally substituted by CN, amide or oxime functions)); R4 = 1-6C alkyl or 2-6C alkenyl (both optionally substituted as in R1-R3); R5 = H or Me; X1-X5 = O, NH, N-(1-6C alkyl), N-(2-6C alkenyl), N-(2-6C alkynyl), N-acyl, N-aryl, N-O-R1 or S. Independent claims are also included for: (1) the preparation of (I); and (2) the new microorganism ST003236 (DSM 14476).

Description

IN THE MATTER OF an Australian Application corresponding to PCT Application PCT/EP02/12420 RWS Group plc, of Europa House, Marsham Way, Gerrards Cross, Buckinghamshire, England, hereby solemnly and sincerely declares that, to the best of its knowledge and belief, the following document, prepared by one of its translators competent in the art and conversant with the English and German languages, is a true and correct translation of the PCT Application filed under No. PCT/EP02/12420.

Date: 26 February 2004 C. E. SITCH Deputy Managing Director UK Translation Division For and on behalf of RWS Group plc (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International publication date May 2003 (30.05.2003) PCT (10) International publication number WO 03/043984 Al (51) International patent classification 7 C07D 207/44 (21) International application number: PCT/EP02/12420 (22) International filing date: 7 November 2002 (07.11.2002) (81) Designated states (national): AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZM, ZW.

Language of filing: German German (26) Language of publication: Data relating to the priority: 101 56906.8 21 November 2001 (21.11.2001) (71) Applicant: AVENTIS PHARMA DEUTSCHLAND GM [DE/DE]; Briiningstrasse 50, 65929 Frankfurt am Main (D] (72) Inventors: SCHIELL, Matthias; Zehntenstrasse 1, 65 Brechen KNAUF, Martin; Bahnhofstrasse 5, Post 331, CH-6037 Root TOTI, Luigi; Frankfurter Strass 65239 Hochheim MARKUS-ERB, Astrid; Sulzbai Strasse 6, 65835 Liederbach (DE).

(84) Designated states (regional): ARIPO Patent (GH, GM, DE KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian Patent (AM, AZ, BY, KG, KZ, MD, RU, TJ, [BH TM), European Patent (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SK, TR), OAPI Patent (BF, BJ, CF, CG, CI, CM, 611 GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).

fach e 5, Published: cher With the International Search Report.

Before expiry of the period provided for amending he claims, will be republished if such amendments re received.

For an explanation of the two-letter codes and the other abbreviations, reference is made to the explanations ("Guidance Notes on Codes and Abbreviations") at the beginning of each regular edition of the PCT Gazette.

As printed (54) Title: GABUSECTIN DERIVATIVES, METHOD FOR THE PRODUCTION THEREOF AND USE OF THE SAME (54) Bezeichnung: GABUSECTIN-DERIVATE, VERFAHREN ZUR DEREN HERSTELLUNG UND DEREN VERWENDUNG (57) Abstract: The invention relates to compounds of formula which are formed by the micro-organism ST 003236 (DSM 14476) during fermentation.

The invention also relates to a method for producing said compounds and to the derivation thereof, to pharmaceuticals containing a compound of formula and to the use of the same for producing a pharmaceutical.

S"4 (57) Zusammenfassung: Gabusectin-Derivate.

O Verfahren zur deren Herstellung und deren Rlm Verwendung Die vorliegende Erfindung betrifft H C 5 Verbindungen der Formel die von dem

CH

3 Mikroorganismus ST 003236 (DSM 14476) Swiihrend der Fermentation gebildet werden, ein SVerfahren zu ihrer Herstellung und Derivatisierung, Arzneimittel enthallend eine Verbindung der Formel und Verwendung derselhen zur Herstellung eines Arzneimittels.

WO 03/043984 PCT/EP02/12420 Gabusectin derivatives, processes for preparing them and their use A large number of antibiotics are used therapeutically for treating infectious diseases of bacterial origin. However, the pathogens are becoming increasingly resistant to the pharmaceuticals employed; Even what are termed multiresistant organisms, which have become resistant not only to individual antibiotic groups, such as 3-lactam antibiotics, glycopeptides or macrolides, but also carry several resistances simultaneously, pose a great threat. There are even pathogens which have become resistant to all the commercially available antibiotics. Infectious diseases which are caused by these organisms can no longer be treated. There is therefore a great need for novel medicines which can be used against resistant organisms. While many thousand antibiotics have been described in the literature, most of them are too toxic to be able to be used as pharmaceuticals.

A relatively large number of antibiotics having a tetramic acid basic structure have already been described. Tetramic acid, i.e. 2,4-pyrrolidinedione, is the parent compound for a variety of natural products which are formed by some microorganisms and marine invertebrates.

harzianic acid, an antibiotic which possesses very little activity, was described in 1994 Sawa et al., J. Antibiotics, 47, 731-732, 1994); The natural tetramic acid derivatives which were published up until 1994 are described in a review by B.J. L. Royles (Chem. Rev. 95, pages 1981 2001, 1995).

Further natural tetramic acids, some of which possess antibacterial properties, have been described since 1995: reutericyclin H6ltzel et al., Angew. Chem. 112, 2886-2888, 2000), a compound which possesses slight antibacterial activity; equisetin and phomasetin S. Singh et al., Tetrahedron Lett. 39, 2243-2246, 1998) are isomeric inhibitors of HIV-1 integrase; cryptocin Y. Li et al., Org. Lett. 2, 767-770, 2000), which is an antimycotic compound; vancoresmycin V. S. Ramakrishna et al., Int. Patent Publication No.

WO 0028064), an antibiotic; 00 O* coniosetin Vertesy et al., German patent application No. DE c 10060810.8), a potent antibiotic composed of a tetramic acid moiety and a naphthyl moiety.

It has been found, surprisingly, that the strain ST 003236 (DSM 14476) is S able to form the novel antibiotic gabusectin, which is not only very active against bacteria but is also well tolerated.

ID

CThe invention accordingly relates to the compounds which are formed by 0 10 the strain ST 003236 (DSM 14476) and to their physiologically tolerated salts, stereoisomers, tautomers, derivatives, in particular ester derivatives, and obvious chemical equivalents, such as ethers.

The invention relates to isolated compounds of the formula (I) RX (I) R3 H3 2 C R

CHR

where R, R 2 and R 3 are, independently of each other: 1. H, or 2. C 1

-C

6 -alkyl, C 2

-C

6 -alkenyl or C 2

-C

6 -alkynyl, in which alkyl, alkenyl and alkynyl are straight-chain or branched and are optionally substituted, once or twice, by: 2.1 -OH, 2.2 =0, 2.3 -O-C 1

-C

6 -alkyl, in which alkyl is straight-chain or branched, 2.4 -O-C2-C6-alkenyl, in which alkenyl is straight-chain or branched, -aryl, 2.6 -NH-C1-C 6 -alkyl, in which alkyl is straight-chain or branched, 2.7 -NH-C 2 -C6-alkenyl, in which alkenyl is straight-chain or branched, 2.8 -NH 2 or 2.9 halogen, in which the substituents 2.3 to 2.7 can be further substituted by -CN, -amide or -oxime functions,

R

4 is C1-C 6 -alkyl or C2-C6-alkenyl, in which alkyl and alkenyl can be straightchain or branched and are optionally substituted once or twice, as described under 2.1 to 2.9,

R

5 is H or methyl, X, X 2

X

3

X

4 and X 5 are, independent of each other O, NH, N-C1-C 6 -alkyl, N-

C

2

-C

6 -alkenyl, N-C 2

-C

6 -alkynyl, N-acyl, N-aryl, N-O-R or S, or a stereoisomeric form or a tautomeric form of the compound of the formula or a mixture of the previously mentioned forms in any ratio, or a physiologically tolerated salt of the compound of the formula or of a stereoisomeric form or of a tautomeric form of a compound of the formula

C

1

-C

6 -alkyl is a straight-chain or branched alkyl having from 1 to 6 C atoms, preferably having from 1 to 4 C atoms, e.g. methyl, ethyl, i-propyl, tert-butyl and hexyl.

C

2

-C

6 -alkenyl is a straight-chain or branched alkenyl which has from 2 to 6 C atoms, and which is unsaturated once, twice or three times, e.g. allyl, crotyl, 1-propenyl, penta-1,3-dienyl and pentenyl.

00 0 C 2

-C

6 -alkynyl is a straight-chain or branched alkynyl which has from 2 to 6 C Nc atoms, and which is saturated once or twice, eg. propynyl, butynyl and pentynyl.

Ct Aryl is phenyl, benzyl or 1- or 2-naphthyl, which can also be additionally substituted, for example by halogen, such as chlorine, bromine, fluorine, by alkyl having 1-4 C atoms, preferably methyl-, by hydroxyl, by alkoxy having 1-4 C atoms, in particular methoxyl, or by trifluoromethyl.

IND

S Acyl can be aliphatic or aromatic acyl radicals. Aliphatic acyl has 1-7, 0 10 preferably 1-4, C atoms, such as formyl, acetyl, propionyl, butyryl, hexanoyl, acryloyl, crotonoyl, or propioloyl, which can be still further substituted, for example by halogen, such as chlorine, bromine or fluorine, by amino, or by alkylamino having 1-4 C atoms, preferably methyl or ethylamino groups. Aromatic acyl can, for example, be benzoyl or naphthoyl which can also be additionally substituted, for example by halogen, such as chlorine, bromine or fluorine, by alkyl having 1-4 C atoms, preferably methyl, by hydroxyl, by amino groups, such as ethylamino, or by alkoxy groups having 1-7, preferably 1-4, C atoms, in particular methoxy.

The invention preferably relates to an isolated compound of the formula where R is 1.0 H, or

C

1

-C

6 -alkyl, C 2

-C

6 -alkenyl or C 2

-C

6 -alkynyl, in which alkyl, alkenyl and alkynyl are straight-chain or branched and are optionally substituted once or twice by: 2.1 -OH, 2.2 =0, 2.3 -O-C1-C 6 -alkyl, in which alkyl is straight-chain or branched, 2.4 -O-C 2

-C

6 -alkenyl, in which alkenyl is straight-chain or branched, -aryl, 2.6 -NH-C 1

-C

6 -alkyl, in which alkyl is straight-chain or branched, 2.7 -NH-C 2

-C

6 -alkenyl, in which alkenyl is straight-chain or branched, 2.8 -NH 2 or 2.9 halogen, in which the substituents 2.3 to 2.7 can be additionally substituted by -CN, -amide or-oxime functions,

R

2 is H,

R

3 is CH 3

R

4 is-CH=CH-CH 3

R

5 is CH 3 and X, X 2

X

3

X

4 and X 5 are 0.

Particularly preferably, the invention relates to a compound of the formula where R is H,

R

2 is H or CH 3

R

3 is CH 3

R

4 is -CH=CH-CH 3

R

5 is CH 3 and X, X 2

X

3

X

4 and X 5 are 0.

Tautomeric forms of the compound are, for example, a compound of the formula (II) R3 R2X3 N

XR

C H 3 X

(I)

H3,C R 3 where the radicals R, R 2

R

3

R

4

R

5 X, X 2

X

3

X

4 and X 5 are defined as above, where tautomeric forms of the compounds of the formula result, for example, from the hydrogen-bonded tetramic acid structural moiety, 6 00 S3 R3 1O N O N

H

X0

X

S and are converted into each other in solution in dependence on parameters such as pH and solvent polarity.

CN SUnless otherwise indicated, chiral centers in the compounds of the formulae and (II) can be present in the R configuration or in the S configuration. The invention relates both to the optically pure compounds and to stereoisomeric mixtures, such as enantiomeric mixtures and diasteromeric mixtures, in any ratio.

Of the compounds of the formulae and (II) according to the invention, preference is given to those compounds in which the configuration corresponds to the substituted hydrogenated naphthyl backbone of the formula (III): H ~.CH 3 3 R4 1 l ll)

H

3

C

H, R,

CH

3 The invention furthermore relates to an isolated compound of the formula (IV), CH3 13 O N 0 HO 0

OH

CH3

(IV)

lo'CH3 to an isolated compound of the formula

IND

IND

H3C 'CH 3 CH3 to an isolated compound of the formula (VI),

O-CH

3

(VI)

H3C to an isolated compound of the formula (VII),

CH

3 1

O-CH

3

(VII)

H3C or to a stereoisomeric form or a tautomeric form of a compound of the formula (IV), (VI) or (VII) or to a mixture of the respective previously mentioned forms in any ratio, or to a physiologically tolerated salt of a compound of the formula (VI) or (VII) or of a stereoisomeric form or of a tautomeric form of a compound of the formula (VI) or (VII).

The inventive compounds differs from substances which are known from the literature, for example in their polarity, their chemical structure or their antimicrobial activity or other physical properties. In particular, as compared with the compounds in the prior art, the compounds according to the invention contain an additional methyl group in the naphthyl moiety.

The invention furthermore relates to obvious chemical equivalents of the compounds of the formulae to (VII).

Obvious chemical equivalents of the compounds according to the invention are compounds which possess the same activity as the compounds according to the invention and exhibit a trivial chemical difference or which are converted, under mild conditions, into the compounds according to the invention. Said equivalents include, for example, esters, azomethines (Schiff's bases), ketals, oximes, hydrogenation products, reduction products, complexes or addition compounds of or with the compounds according to the invention.

For example, an activated acid, for example acid chlorides or other acid derivatives, can be reacted with the hydroxyl group of the compound of the formula or of one or more double bonds and/or carbonyl groups of the compound of the formula can be reduced with a reducing agent, with double bonds being reduced, for example, using H 2 /Pd and carbonyl groups being reduced, for example, using NaBH 4 The abovementioned methods for derivatizing are described in text books such as Jerry March, Advanced Organic Chemistry, John Wiley Sons, 4 th Edition, 1992. In order to carry out reactions selectively, it can be advantageous to introduce suitable protecting groups, in a manner known per se, prior to the reaction. The protecting groups are eliminated after the reaction and the reaction product is subsequently purified.

The invention furthermore relates to gabusectin, a compound which has the empirical formula C 2 5

H

35 N0 4 as demonstrated by ESI and FAB mass spectroscopy, and which is characterized by the 1H NMR and 1 3 C NMR data given in table 2, or to a stereoisomeric form or a tautomeric form of the compound gabusectin, or to a mixture of the respective previously mentioned forms in any ratio, or to a physiologically tolerated salt of the compound gabusectin or of a stereoisomeric form or of a tautomeric form of the compound gabusectin.

The invention furthermore relates to gabusectin methyl ester, a compound of the empirical formula C 27

H

39 N0 5 demonstrated by ESI and FAB mass spectroscopy, and characterized by the 1H NMR and 13 C NMR data given in table 3, or to a stereoisomeric form or a tautomeric form of the compound gabusectin methyl ester, or to a mixture of the respective previously mentioned forms in any ratio, or to a physiologically tolerated salt of the compound gabusectin methyl ester or of a stereoisomeric form or a tautomeric form of the compound gabusectin methyl ester.

The invention furthermore relates to a compound of the formula which can be obtained by fermenting ST 003236 (DSM 14476), or a variant and/or mutants of ST 003236 (DSM 14476), in a culture medium until the compound of the formula (I) accumulates in the culture broth, then isolating the compound of the formula and, where appropriate, converting it into a pharmacologically tolerated salt.

The invention also relates to a compound of the empirical formula C 2 6

H

3 7 N0 (Gabusectin) which can be obtained by fermenting ST 003236 (DSM 14476), or a variant and/or mutant of ST 003236 (DSM 14476) in a culture medium until the compound gabusectin accumulates in the culture broth, subsequently isolating the compound Gabusectin and, where appropriate, converting it into a pharmacologically tolerated salt.

The invention additionally relates to a process for preparing a compound of the formula which comprises culturing the microorganism ST 003236 (DSM 14476), or a variant and/or mutant of ST 003236 (DSM 14476), in an aqueous nutrient medium, isolating and purifying a compound of the formula and, where appropriate, converting it into an obvious chemical equivalent or a pharmacologically tolerated salt.

The invention furthermore relates to a process for preparing a compound of the formula which comprises esterifying gabusectin of the formula (IV) with a C 1

-C

6 alkyl-, C 2

-C

6 -alkenyl- or C 2

-C

6 -alkynyl-alcohol derivative, or with a C 1

-C

6 -alkyl-, C2-C6-alkenyl- or C2-C6-alkynyl-alkylating agent, to give a compound of the formula in which alkyl, alkenyl and alkynyl are straight-chain or branched and can optionally be substituted, once or twice, by the radicals 2.1 to 2.9 in accordance with formula in claim 1, in which the substituents 2.3 to 2.7 can be further substituted by -CN, -amide or -oxime functions, and R 2 is H, R 3 is CH 3

R

4 is -CH=CH-CH 3

R

5 is CH 3 and X, X 2

X

3

X

4 and X 5 are 0, preferably using a Cl-C6-alkylalkylating agent, particularly preferably using a C 1 -alkylating agent.

C

1

-C

6 -Alkyl-, C 2

-C

6 -alkenyl- or C 2

-C

6 -alkynyl-alcohol derivatives are straight-chain or branched and optionally substituted once or twice by the radicals 2.1 to 2.9, see above, in which the substituents 2.3 to 2.7 can be further substituted by -CN, -amide or -oxime functions, for example methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, tert-butanol and n-hexanol, 2-buten-l-ol (crotyl alcohol), 1-propen-3-ol (allyl alcohol), 1,3-pentadien-5-ol, 1,4-pentadien-3-ol and 2-penten-1ol, 1-penten-4-ol (allylmethylcarbinol), 1-penten-3-ol (ethylvinylcarbinol), 2-propyn-1ol (propargyl alcohol), 1-butyn-3-ol, 2-butyn-l-ol, 3-butyn-l-ol, 1-pentyn-3-ol, 2-pentyn-l-ol, 3-pentyn-l-ol and 4-pentyin-l-ol, preferably methanol.

CI-C6-Alkyl-, C 2

-C

6 -alkenyl- or C 2

-C

6 -alkynyl-alkylating agents are straight-chain or branched and optionally substituted once or twice by the radicals 2.1 to 2.9, see above, for example diazomethane derivatives as Cl-alkylating agents, for example trimethylsilyldiazomethane.

Methods for esterifying are described, for example, in Jerry March, Advanced Organic Chemistry, John Wiley Sons, 4 th Edition, 1992.

The strain ST 003236 has been deposited in the Deutsche Sammlung von Microorganismen und Zellkulturen [German collection of microorganisms and cell cultures] GmbH (DSM), Mascheroder Weg 1B, 38124 Braunschweig, Germany, in accordance with the rules of the Budapest Treaty, under the following number DSM 14476.

Said process comprises culturing ST 003236 (DSM 14476), its mutants or variants, under aerobic conditions in a culture media containing one or more carbon and nitrogen sources, inorganic salts and, where appropriate, trace elements.

The course of the fermentation, and the formation of the antibiotics according to the invention, can be monitored using methods known to a skilled person, for example by testing the biological activity in bioassays or by means of chromatographic methods such as thin layer chromatography (TLC) or high performance liquid chromatography

(HPLC).

A mutant is a microorganism in which one or more genes in the genome has/have been modified, with the gene or genes which is/are responsible for the ability of the organism to produce the compound according to the invention remaining functional and inheritable.

Such mutants can be generated, in a manner known per se, by physical means, for example irradiation, such as with ultraviolet rays or X-rays, or using chemical mutagens, such as ethyl methanesulfonate (EMS); 2-hydroxy-4-methoxy-benzophenone (MOB) or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), or as described by Brock et al. in "Biology of Microorganisms", Prentice Hall, pages 238-247 (1984).

A variant is a phenotype of the microorganism. Microorganisms have the ability to adapt to their environment and therefore demonstrate pronounced physiological flexibility. In phenotypic adaptation, all the cells in the microorganism are involved, with the nature of the change not being genetically conditioned and being reversible under altered circumstances Stolp, Microbial ecology: organism, habitats, activities. Cambridge University Press, Cambridge, GB, page 180, 1988).

Screening for mutants and variants which produce the antibiotic according to the invention can be carried out by determining the biological activity of the active compound which has accumulated in the culture broth, for example by determining its antibacterial effect, or by detecting compounds, which are known to be antibacterially active, in the fermentation broth using HPLC or LC-MS methods, for example.

The compound gabusectin is found both in the mycelium and in the culture filtrate. It is therefore expedient to separate the fermentation solution into the culture filtrate and the mycelium by means of filtration and to dry these fractions separately. The dried culture filtrate and the dried mycelium are expediently extracted separately with an organic solvent, for example methanol or 2-propanol.

While the extraction can be carried out over a wide pH range, it is expedient to carry it out in a neutral or weakly acidic medium, preferably between pH 3 and pH 7. The extract can, for example, be concentrated and dried in vacuo.

One method of isolating the antibiotic according to the invention proceeds in accordance with the polarity separation principle, in a manner known per se.

Another method of purification is chromatography on adsorption resins, for example on Diaion® HP-20 (Mitsubishi Casei Corp., Tokyo), on Amberlite® XAD 7 (Rohm and Haas, USA), on Amberchrom® CG, (Toso Haas, Philadelphia, USA) or on similar materials. A large number of reverse-phase supports, such as RP 8 and RP 18 as have become well known, for example, within the context of high pressure liquid chromatography (HPLC), are also suitable.

Another possibility for purifying the compound according to the invention is that of using what are termed normal-phase chromatography supports, such as silica gel or A1 2 0 3 or other supports, in a manner known per se.

An alternative isolation method is that of using molecular sieves, such as Fractogel® TSK HW-40 (Merck, Germany) and others, in a manner known per se. In addition to this, it is also possible to isolate the gabusectin by crystallization from enriched material. Organic solvents and their mixtures, either anhydrous or containing added water, are, for example, suitable for this purpose. An additional method for isolating and purifying the antibiotics according to the invention is that of using anion exchangers, preferably in a pH range of from 4 to 10, and cation exchangers, preferably in a pH range of from 2 to 5. The use of buffer solutions to which quantities of organic solvents have been added is particularly suitable for this purpose.

Gabusectin, the said chemical derivatives thereof, and the obvious chemical equivalents thereof, can be converted into the corresponding pharmacologically tolerated salts using methods known to a skilled person.

Pharmacologically tolerated salts of the compounds according to the invention are understood as being both inorganic and organic salts, as are described in Remington's Pharmaceutical Sciences (17th edition, page 1418 [1985]). Suitable salts are, in particular, alkali metal salts, ammonium salts, alkaline earth metal salts, salts with physiologically tolerated amines and salts with inorganic or organic acids, such as HCI, HBr, H 2 S0 4 maleic acid, and fumaric acid.

It has been found, surprisingly, that the compounds of the formula according to the invention exhibit antibacterial effects and are therefore suitable for the treatment of diseases which are caused by bacterial infection. Table 1 summarizes the minimum inhibitory concentrations (MICs) of gabusectin, by way of example.

Table 1: In-vitro antibacterial activity of the compound gabusectin in a serial dilution test.

Bacterium (strain) MIC values (pg/ml) S.aureus (SG511) S.aureus (Exp54146) S.pyogenes (A561) E.faecium (M78L) Gabusectin is well-tolerated at and above its effective concentration.

The present invention therefore also relates to the use of one or more of the compounds of the formula to (VII) according to the invention as pharmaceuticals, and the use of one or more of the compounds of the formula to (VII) according to 00 O the invention for producing pharmaceuticals, in particular for the treatment and/or Sprophylaxis of bacterial infections.

The present invention furthermore relates to a pharmaceutical which has a content of one or more compounds according to the invention.

Said pharmaceutical comprising a compound of the formula is produced

IND

IDusing one or more physiological auxiliary substances and brought into a suitable Sadministration form.

C The pharmaceuticals according to the invention can be used enterally (orally), parenterally (intramuscularly or intravenously), rectally or locally (topically). They can be administered in the form of solutions, powders (tablets and capsules, including microcapsules), ointments (creams or gels), or suppositories. Suitable auxiliary substances for such formulations are the pharmaceutically customary liquid or solid fillers and extenders, solvents, emulsifiers, glidants, taste corrigents, dyes and/or buffering substances. 0.1 1 000, preferably 0.2 100, mg/kg of body weight is/are administered as an expedient dose. The doses are expediently administered in dosage units which contain at least the effective daily quantity of the compounds according to the invention, for example 30 3 000, preferably 50 1 000, mg.

Comprises/comprising and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features, integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.

The following examples are intended to be used for clarifying the invention without in any way restricting its scope.

Example 1 Preparing a glycerol culture of ST 003236 (DSM 14476).

14a 00 0 30 ml of nutrient solution (malt extract, yeast extract, glucose, (NH4) 2

HPO

4 0.05%, pH 6.0) were inoculated with the strain ST 003236 S(DSM 14476) in a sterile 100 ml Erlenmeyer flask and incubated for 6 days, at and 140 rpm, on a rotating shaker. 1.5 ml of this culture were then diluted with 2.5 ml of 80% glycerol and stored at -135°C.

Example 2 Preparing a preliminary culture of ST 003236 (DSM 14476) in I an Erlenmeyer flask.

IN

100 ml of nutrient solution (malt extract, yeast extract, glucose, (NH4) 2 HP04, 0.05%, pH 6.0) were inoculated with an ampoule of the strain ST 003236 (DSM 14476) in a sterile 300 ml Erlenmeyer flask and incubated for 6 days at 25°C and 140 rpm. 2 ml of this preliminary culture were subsequently inoculated for preparing the main cultures.

Example 3 Preparing a liquid main culture of ST 003236 (DSM 14476).

A sterile 300 ml Erlenmeyer flask containing 100 ml of the following nutrient solution: potato dextrose, yeast extract, was inoculated with a culture grown on a sloping tube (same nutrient solution but containing 2% agar) or with 2 ml of a preliminary culture (see example 2) and incubated, at 140 rpm and 25°C, on a shaker. The maximum production of one or more compounds of the formula (I) according to the invention was reached after approx. 144 hours. A 96 hour-old submerged culture from the same nutrient solution (inoculation quantity, approx.

was adequate for inoculating fermenters of from 10 to 200 I in volume. The conditions for these fermenters were: Temperature: 25 0

C

Stirrer speed: 200 rpm Aeration: 15 I. Min-.

It was possible to suppress foam formation by repeatedly adding ethanolic polyol solution. The production maximum was achieved after approx. 96 to 144 hours.

Example 4: Isolating the compound gabusectin.

3 I of culture solution, obtained as described in example 3, were filtered and the culture filtrate and the mycelium were freeze-dried separately. The dried culture filtrate was extracted with 3 liters of methanol. The clear liquid phase was concentrated down to 200 ml in vacuo and filtered. This methanol solution was mixed with water in a ratio of 9:1 in an HPLC high pressure gradient unit and loaded onto a 300 ml-capacity column filled with the adsorption resin MCI Gel® (Mitsubishi Casei Corp., Tokyo). Column dimensions: width x height: 5 cm x 15 cm.

The column was eluted with a solvent gradient of water to 100% methanol and the outflow from the column (50 ml/minute) was collected in fractions of in each case ml in volume. The gabusectin-containing fractions 65 to 75, which were checked by HPLC analyses, were collected and concentrated in vacuo and freeze-dried (0.23 g).

Example 5: Purifying gabusectin by high pressure liquid chromatography (HPLC).

Column: Purospher STAR RP-18 e 3 pm, 30-2, (Merck, Germany) Mobile phase buffer A: 5% acetonitrile 0.1% ammonium acetate, Mobile phase buffer B: 95% acetonitrile 0.1% ammonium acetate, Gradient: 15 min Flow rate: 0.25 ml per minute Detection by UV absorption at 210 nm.

Gabusectin was found to have a retention time of 6.5 min.

Example 6: Final purification of gabusectin.

The enriched antibiotic gabusectin (0.23 obtained as described in example 4, was fractionated on a LUNA® 10 pm C 18(2) HPLC column (Phenomenex, USA) (width x height 2.1 cm x 25 cm) by the gradient method using from 5% to 95% acetonitrile in 0.05% trifluoroacetic acid. Flow rate: 25 ml/min. Fraction size: 25 ml. Fraction 48, which was examined by analytical HPLC (see example 5) was freeze-dried. It yielded mg of gabusectin at 98% purity.

Example 7: Characterizing gabusectin.

The physicochemical and spectroscopic properties of the antibiotic according to the invention can be summarized as follows: Appearance: Colorless to pale yellow substance which is soluble in medium-polar and polar organic solvents but not particularly soluble in water. Stable in neutral and mildly acidic medium but unstable in strongly acidic and strongly alkali solution.

Empirical formula: Molecular weight: 1 H NMR and 13C NMR: UV maxima:

C

2 6

H

37 443.59 see table 2 236 nm and 294 nm Determining the molar peak: The mass of 443 is assigned to the sought-after molecule on the basis of the following findings: ESI spectrum and FAB spectra exhibit peaks at 444 amu (M+H) ESI spectrum exhibits a peak at 442 amu High resolution of the quasi molecule ion: FAB 444.27424 (M+H) 443.59 was calculated for the empirical formula C 2 6

H

3 7 N0 5 Table 2: 1H- and 1 3C-chemical shifts of gabusectin in CDCI 3 at 275K.

OH

H

3

C

Position 13C 6 (ppm) 1H (ppm) HMBC correlations (13C-1H) 1 49.01 H3-Me(w), H1-Me, H2(w), H10, 14-OH 1-Me 20.77 1.24 s 2 45.69 3.36 H3-Me, H9',H1-Me, H10, H4, H12(s), H11(w) 3 132.86 H11(w), H2, H3-Me(s), H6'(w) Position 13 C 8 (ppm) 1 H 6 (ppm) HMBC correlations 13

C-

1

H)

3-Me 23.43 1.69 s H4 4 130.04 5.06 H2, H10, H3-Me(s), H6', 37.61 H6', H7-Me(w), H5-Me(s), H10, H4(s) 31.91 0.70 H3-Me, H6', 6 51.62 1.36, 0.92 H3-Me(w), H9', H7-Me, H5-Me, H4(w) 7 29.46 1.26 H6(w), H6', H9(w), H7-Me(s), 7-Me 22.41 0.81 d 8 34.97 1.65, 0.87 H9(w), H6(w), H6', H7-Me(s) 9 25.52 1.84, 1.29 42.17 2.66 dd H9', H1-Me, H5-Me, H2(w), H4 11 132.25 5.31 H12, H2(w), H13(s) 12 128.95 5.44 H11, H2, H13(s) 13 17.90 1.69 H12, H11 14 203.37 14-OH, H2, H10, H1-Me 14-OH 17.73 98.81 14-OH 16 177.05 -14-OH, H18(s), H17-Me(s) 17-Me 27.20 3.02s 18 64.27 3.76 dd H17-Me, H20, 19 190.36 H18, H20(w), 23.27 2.32, 2.08 H21, H18 21 27.44 2.30, 2.30 H20, H20', H18 22 178.18 H20, H21 22-COOH 7.1 br Example 8: Inhibitory effect of gabusectin in the agar diffusion test.

Agar plates containing 2 ml of Staphylococcus aureus inoculum in 200 ml of agar solution were prepared. gabusectin was applied, in a 10 mM solution, to 6 mmdiameter paper disks, which were then laid on the agar plate. The inoculated Staphylococcus plates were incubated at 37°C for 16 hours. Inhibition halos having the following diameters (mm) were then observed: Quantity Inhibition halo size (mm) pL 8 pL 14 17 Example 9: Methylation, and subsequent purification of the gabusectin methyl ester.

20 mg of the antibiotic gabusectin (0.045 mmol), obtained as described in example 6, were dissolved in 5 ml of MeOH, after which trimethylsilyldiazomethane was added in a 6-fold molar excess. The reaction mixture was left to stand at room temperature for min and then concentrated to dryness. The resulting mixture was fractionated chromatographically on a LUNA® 5 pm C 18(2) HPLC column (Phenomenex, USA) (width x height 1 cm x 25 cm) by the gradient method using from 5% to acetonitrile in 0.05% trifluoroacetic acid. Flow rate: 6.5 ml/min. Fraction size: 6.5 ml.

Fraction 61, which was examined by analytical HPLC (see example was freezedried. It yielded 7.4 mg of gabusectin methyl ester at 97% purity.

Example 10: Characterizing gabusectin methyl ester.

The physicochemical and spectroscopic properties of the antibiotic according to the invention can be summarized as follows: Appearance: Colorless to pale-yellow substance which is soluble in medium-polar and polar organic solvents but not particularly soluble in water. Stable in neutral and mildly acidic medium but unstable in strongly acidic and strongly alkaline solution.

Empirical formula: C 2 7

H

3 9 N0 Molecular weight: 457.62 1 H NMR and 1 3 C NMR: see table 3 UV maxima: 236 nm and 294 nm Determination of the molar peak: The mass of 457.6 is assigned to the sought-after molecule on the basis of the following findings: ESI spectrum and FAB spectra exhibit peaks at 457 amu (M+H) ESI- spectrum exhibits a peak at 458.5 amu Table 3: 1H and 13C chemical shifts of gabusectin methyl ester in CDCI 3 at 275K

O-CH

3

H

3

C

Position 13 C 8 (ppm) 1 H 6 (ppm) HMBC correlations (13C- 1H) 1 48.89 H3-Me(w), H1-Me, 1-Me 20.83 1.24- 2 45.70 3.36 H3-Me, H1-Me, H9', H10(w), H12, H4 3 132.86 H3-Me 3-Me 23.44 1.68 H4 4 130.08 5.05 H3-Me, H6', H5-Me, 37.63 H6', H5-Me, H10(w), H4(w) 31.91 0.70 H10(w), H6', H3-Me(w) 6 51.65 1-35, 0.92 H9', H7-Me, H5-Me, H4(w) 7 29.48 1.25 H6', H7-Me 7-Me 22.42 0.80 8 35.00 1.64, 0.88 H6', H7-Me(s) 9 25.55 1.83, 1.30 42.17 2.67 H1-Me, H5-Me, H9', H4(w) 11 132.35 5.30 H12, H13 12 128.87 5.43 H11,H13 Position 13C (ppm) 1 H 5 (ppm) HMBC correlations (13C- 1H) 13 17.90 1.69 H12, H11 14 202.86 H1-Me 14-OH 17.75 98.91 16 177.06 H17-Me 17-Me 27.13 3.02 18 64.49 3.71 H20, H20', H21, H17-Me 19 190.33 H18, 23.52 2.29, 2.11 H21 21 27.42 2.23, 2.23 22 173.05 22-OMe, H20, H20', H21 22-OMe 51.93 3.66 Example 11: Inhibitory effect of the gabusectin methyl ester in the agar diffusion test.

Agar plates containing 2 ml of Staphylococcus aureus inoculum in 200 ml of agar solution were prepared. gabusectin methyl ester is applied, in a 10 mM solution, to 6 mm-diameter paper disks, which are then laid on the agar plate. The inoculated Staphylococcus plates were incubated at 37°C for 16 hours. Inhibition halos having the following diameters (mm) were then observed.

Quantity Inhibition halo size (mm) pL 0 pL 7 pL 8

Claims (10)

1. An isolated compound of the formula (I) where R, R 2 and R 3 are, independently of each other: 1. H,or

2. C 1 -C 6 -alkyl, C 2 -C 6 -alkenyl or C 2 -C 6 -alkynyl, in which alkyl, alkenyl and alkynyl are straight-chain or branched and are optionally substituted, once or twice, by: 2.1 -OH, 2.2 =0, 2.3 -O-C 1 -C 6 -alkyl, in which alkyl is straight-chain or branched, 2.4 -O-C 2 -C 6 -alkenyl, in which alkenyl is straight-chain or branched, -aryl, 2.6 -NH-C1-C 6 -alkyl, in which alkyl is straight-chain or branched, 2.7 -NH-C 2 -C 6 -alkenyl, in which alkenyl is straight-chain or branched, 2.8 -NH 2 or 2.9 halogen, in which the substituents 2.3 to 2.7 can be further substituted by -CN, -amide or -oxime functions, R 4 is C1-C6-alkyl or C2-C6-alkenyl, in which alkyl and alkenyl can be straight- chain or branched and are optionally substituted once or twice, as described under 2.1 to 2.9, R 5 is H or methyl, X, X 2 X 3 X 4 and X 5 are, independent of each other O, NH, N-C1-C 6 -alkyl, N- C2-C 6 -alkenyl, N-C2-C6-alkynyl, N-acyl, N-aryl, N-O-R or S, or a stereoisomeric form or a tautomeric form of the compound of the formula or a mixture of the previously mentioned forms in any ratio, or a physiologically tolerated salt of the compound of the formula or of a stereoisomeric form or of a tautomeric form of a compound of the formula 2. A compound of the formula as claimed in claim 1, where R is 1.0 H, or C 1 -C 6 -alkyl, C2-C6-alkenyl or C2-C6-alkynyl, in which alkyl, alkenyl and alkynyl are straight-chain or branched and are optionally substituted once or twice by: 2.1-OH, 2.2=0, 2.3-0-C 1 -C 6 -alkyl, in which alkyl is straight-chain or branched, 2.4-O-C2-C6-alkenyl, in which alkenyl is straight-chain or branched, 2.6-NH-C1-C 6 -alkyl, in which alkyl is straight-chain or branched, 2.7-NH-C2-C6-alkenyl, in which alkenyl is straight-chain or branched, 2.8-NH 2 or 2.9 halogen, in which the substituents 2.3 to 2.7 can be additionally substituted by -CN, -amide or -oxime functions, R 2 is H, R 3 is CH 3 00 O R 4 is -CH=CH-CH 3 0 C R 5 is CH 3 and SX, X2, X3, X4 and X 5 are 0.

3. A compound of the formula as claimed claim 1, where R is H, SR 2 is H or CH 3 N R 3 is CH 3 SR 4 is -CH=CH-CH 3 R 5 is CH 3 and X, X2, X3, X4 and Xs are O.

4. An isolated compound of the formula (IV) CHC 13 HO CH3 (IV) CH or a stereoisomeric form or a tautomeric form of a compound of the formula (IV) or a mixture of the respective previously mentioned forms in any ratio, or a physiologically tolerated salt of a compound of the formula (IV) or of a stereoisomeric form or of a tautomeric form of a compound of the formula (IV).

5. An isolated compound of the formula (V) H 3 C or a stereoisomeric form or a tautomeric form of a compound of the formula or a mixture of the respective previously mentioned forms in any ratio, or a physiologically tolerated salt of a compound of the formula or of a stereoisomeric form or of a tautomeric form of a compound of the formula

6. An isolated compound of the formula (VI) O-CH 3 (VI) or a stereoisomeric form or a tautomeric form of a compound of the formula (VI) or a mixture of the respective previously mentioned forms in any ratio, or a physiologically tolerated salt of a compound of the formula (VI) or of a stereoisomeric form or of a tautomeric form of a compound of the formula (VI).

7. An isolated compound of the formula (VII), CH 3 HO O-CH 3 H CH H O 3 CH (VII) H 3 C H CH 3 CH3 or a stereoisomeric form or a tautomeric form of a compound of the formula (VII) or a mixture of the respective previously mentioned forms in any ratio, or a physiologically tolerated salt of a compound of the formula (VII) or of a stereoisomeric form or of a tautomeric form of a compound of the formula (VII).

8. Gabusectin of the empirical formula C 2 5 H 3 5 N0 4 demonstrated by ESI and FAB mass spectroscopy, and characterized by the 1H NMR data 5 (CDCI 3 275K) 0.70, 0.81, 0.87, 0.92, 1.24, 1.26, 1.29, 1.36, 1.65, 1.69, 1.84, 2.08, 2.30, 2.32, 2.66, 3.02, 3.36, 3.76, 5.06, 5.31, 5.44, 7.1, 17.73, and the 13C NMR data 6 (CDCl 3 275K) 17.90, 20.77, 22.41, 23.27, 23.43, 25.52, 27.20, 27.44, 29.46, 31.91, 34.97,

37.61, 42.17, 45.69, 49.01, 51.62, 64.27, 98.81, 128.95, 130.04, 132.25, 132.86,

177.05, 178.18, 190.36, 203.37, or a stereoisomeric form or a tautomeric form of the compound gabusectin or a mixture of the respective previously mentioned forms in any ratio, or a physiologically tolerated salt of the compound gabusectin or of a stereoisomeric form or of a tautomeric form of the compound gabusectin. 9. Gabusectin methyl ester of the empirical formula C 2 7 H 3 9 N0 5 demonstrated by ESI and FAB mass spectroscopy, and characterized by the 1H NMR data (CDCl 3 275K) 0.70, 0.80, 0.88, 0.92, 1.24, 1.25, 1.30, 1.35, 1.64, 1.68, 1.69, 1.83, 2.11, 2.23, 2.29, 2.67, 3.02, 3.36, 3.66, 3.71, 5.05, 5.30, 5.43, 17.75 and the 13C NMR data 5 (CDCl 3 275K) 17.90, 20.83, 22.42, 23.44, 23.52, 25.55, 27.13, 27.42, 29.48, 31.91, 35.00, 37.63, 42.17, 45.70, 48.89, 51.65, 51.93, 64.49, 98.91, 128.87, 130.08, 132.35, 132.86, 173.05, 177.06, 190.33, 202.86, or a stereoisomeric form or a tautomeric form of the compound gabusectin methyl ester or a mixture of the respective previously mentioned forms in any ratio, or a physiologically tolerated salt of the compound gabusectin methyl ester or of a stereoisomeric form or of a tautomeric form of the compound gabusectin methyl ester. A compound of the formula which can be obtained by fermenting ST 003236 (DSM 14476), or a variant and/or mutant of ST 003236 (DSM 14476) in a culture medium until the compound of the formula accumulates in the culture broth, subsequently isolating the compound of the formula and, where appropriate, converting it into a pharmacologically tolerated salt. 11. A compound of the empirical formula C 2 6 H 37 N0 5 (gabusectin) which can be obtained by fermenting ST 003236 (DSM 14476) or a variant and/or mutant of ST 003236 (DSM 14476), in a culture medium until the compound gabusectin accumulates in the culture broth, subsequently isolating the compound gabusectin and, where appropriate, converting it into a pharmacologically tolerated salt. 12. A process for preparing the compound of the formula which comprises culturing the microorganism ST 003236 (DSM 14476), or a variant and/or mutant of ST 003236 in an aqueous nutrient medium, isolating and purifying a compound of the formula and, where appropriate, converting it into an obvious chemical equivalent and/or a pharmacologically tolerated salt. 13. The process as claimed in claim 12, wherein the microorganism ST 003236 (DSM 14476) or a mutant and/or variant of ST 003236 is fermented, under aerobic conditions, in a culture medium which contains a carbon source and a nitrogen source and also the customary inorganic salts and trace elements. 14. The process as claimed in either or both claims 12 and 13, wherein the fermentation under aerobic conditions is carried out at a temperature between 20 and 0 C and at a pH of between 4 and A process for preparing a compound of the formula as claimed in claim 1, which comprises esterifying gabusectin of the formula (IV) as claimed in claim 4, with 00 O Cl a C 1 -C 6 -alkyl-, C 2 -C6-alkenyl- or C2-C 6 -alkynyl-alcohol derivative or with a CI-C6- Salkyl-, C2-C 6 -alkenyl- or C 2 -C6-alkynyl-alkylating agent to give a compound of the formula as claimed in claim 1, in which alkyl, alkenyl and alkynyl are straight- chain or branched and can be optionally substituted once or twice by the radicals 2.1 to 2.9, in which the substituents 2.3 to 2.7 can be further substituted by -CN, -amide or -oxime functions, and R 2 is H, R 3 is CH 3 R 4 is -CH=CH-CH 3 R 5 is O CH 3 and X, X 2 X 3 X 4 and X 5 are O. NO S16. The use of a compound as claimed in one or more of claims 1 to 11, for c N producing a pharmaceutical. 17. A pharmaceutical having a content of at least one compound as claimed in one or more of claims 1 to 11 and of one or more physiologically suitable auxiliary substances. 18. A process for producing a pharmaceutical as claimed in claim 17, which comprises bringing at least one compound as claimed in one or more of claims 1 to 11, together with one or more physiologically suitable auxiliary substances, into a suitable administration form. 19. The microorganism ST003236 (DSM 14476). An isolated compound of formula (VI) or (VII) substantially as hereinbefore described with reference to the Examples. 21. A process for producing an isolated compound according to claim substantially as hereinbefore described with reference to the Examples. SANOFI-AVENTIS DEUTSCHLAND GMBH WATERMARK PATENT TRADEMARK ATTORNEYS P23973AU00