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AU2002218129A1 - Method for the detection of cytosine methylations - Google Patents

Method for the detection of cytosine methylations

Info

Publication number
AU2002218129A1
AU2002218129A1 AU2002218129A AU1812902A AU2002218129A1 AU 2002218129 A1 AU2002218129 A1 AU 2002218129A1 AU 2002218129 A AU2002218129 A AU 2002218129A AU 1812902 A AU1812902 A AU 1812902A AU 2002218129 A1 AU2002218129 A1 AU 2002218129A1
Authority
AU
Australia
Prior art keywords
cytosine
detection
amplified
genomic dna
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002218129A
Other languages
English (en)
Inventor
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of AU2002218129A1 publication Critical patent/AU2002218129A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Control Of Turbines (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2002218129A 2000-10-10 2001-10-10 Method for the detection of cytosine methylations Abandoned AU2002218129A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10050942A DE10050942B4 (de) 2000-10-10 2000-10-10 Verfahren zum Nachweis von Cytosin-Methylierungen
DE10050942.8 2000-10-10
PCT/DE2001/003901 WO2002031186A2 (de) 2000-10-10 2001-10-10 Verfahren zum nachweis von cytosin-methylierungen

Publications (1)

Publication Number Publication Date
AU2002218129A1 true AU2002218129A1 (en) 2002-04-22

Family

ID=7659773

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002218129A Abandoned AU2002218129A1 (en) 2000-10-10 2001-10-10 Method for the detection of cytosine methylations

Country Status (7)

Country Link
EP (1) EP1325154B1 (de)
JP (1) JP2004511235A (de)
AT (1) ATE368752T1 (de)
AU (1) AU2002218129A1 (de)
CA (1) CA2425366A1 (de)
DE (2) DE10050942B4 (de)
WO (1) WO2002031186A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10061348C2 (de) * 2000-12-06 2002-10-24 Epigenomics Ag Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA
DE10151055B4 (de) 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
AU2003236461B2 (en) 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
JP2006516391A (ja) 2003-01-29 2006-07-06 エフ．ホフマン−ラ　ロシュ　アーゲー 重亜硫酸塩処理の改良された方法
US7262013B2 (en) 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
JP4571643B2 (ja) * 2003-10-09 2010-10-27 エピゲノミクス アーゲー 改良された重亜硫酸塩によるｄｎａの変換
EP1692315A1 (de) * 2003-12-02 2006-08-23 Roche Diagnostics GmbH Verbessertes verfahren zur bisulfitbehandlung
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
EP1794319B1 (de) * 2004-09-30 2014-08-13 Epigenomics AG Verfahren zur bereitstellung von dna-fragmenten aus einer archivierten probe
US7658288B2 (en) 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP1869215B1 (de) 2005-04-01 2014-05-07 Epigenomics AG Verbesserte bisulfit-umwandlung von dna
DE602006016859D1 (de) 2005-12-14 2010-10-21 Roche Diagnostics Gmbh Neues verfahren für die bisulfitbehandlung
WO2009113303A1 (ja) 2008-03-12 2009-09-17 国立大学法人北陸先端科学技術大学院大学 光応答性プローブを用いたメチルシトシンの検出法
US9624530B2 (en) * 2009-08-03 2017-04-18 Epigenomics Ag Methods for preservation of genomic DNA sequence complexity

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
DE19853398C1 (de) * 1998-11-19 2000-03-16 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischer DNA
DE19905082C1 (de) * 1999-01-29 2000-05-18 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben

Also Published As

Publication number Publication date
ATE368752T1 (de) 2007-08-15
DE10050942B4 (de) 2005-11-17
CA2425366A1 (en) 2002-04-18
DE10050942A1 (de) 2002-04-18
WO2002031186A2 (de) 2002-04-18
WO2002031186A3 (de) 2003-03-13
EP1325154B1 (de) 2007-08-01
JP2004511235A (ja) 2004-04-15
EP1325154A2 (de) 2003-07-09
DE50112799D1 (de) 2007-09-13

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application