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AU2001238458A1 - Improved assay for protein tyrosine phosphatases - Google Patents

Improved assay for protein tyrosine phosphatases

Info

Publication number
AU2001238458A1
AU2001238458A1 AU2001238458A AU3845801A AU2001238458A1 AU 2001238458 A1 AU2001238458 A1 AU 2001238458A1 AU 2001238458 A AU2001238458 A AU 2001238458A AU 3845801 A AU3845801 A AU 3845801A AU 2001238458 A1 AU2001238458 A1 AU 2001238458A1
Authority
AU
Australia
Prior art keywords
protein tyrosine
tyrosine phosphatases
improved assay
assay
improved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001238458A
Inventor
Deborah E. Cool
Andrew J. Flint
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ceptyr Inc
Original Assignee
Ceptyr Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ceptyr Inc filed Critical Ceptyr Inc
Publication of AU2001238458A1 publication Critical patent/AU2001238458A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/912Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205Phosphotransferases in general
    • G01N2333/9121Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/04Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/20Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001238458A 2000-02-14 2001-02-13 Improved assay for protein tyrosine phosphatases Abandoned AU2001238458A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18176900P 2000-02-14 2000-02-14
US60/181,769 2000-02-14
PCT/US2001/005180 WO2001061031A2 (en) 2000-02-14 2001-02-13 Improved assay for protein tyrosine phosphatases

Publications (1)

Publication Number Publication Date
AU2001238458A1 true AU2001238458A1 (en) 2001-08-27

Family

ID=22665713

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001238458A Abandoned AU2001238458A1 (en) 2000-02-14 2001-02-13 Improved assay for protein tyrosine phosphatases

Country Status (5)

Country Link
US (2) US20020009762A1 (en)
EP (1) EP1257824A2 (en)
AU (1) AU2001238458A1 (en)
CA (1) CA2435507A1 (en)
WO (1) WO2001061031A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001064939A2 (en) * 2000-03-02 2001-09-07 Morphochem Ag Process for obtaining inhibitors/activators of an enzyme
WO2003025199A2 (en) 2001-09-18 2003-03-27 Millennium Pharmaceuticals, Inc. Mid 4460, a human tyrosine phosphatase family member and uses therefor
AU2003299466A1 (en) * 2002-05-03 2004-06-07 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US20040171034A1 (en) * 2002-05-03 2004-09-02 Brian Agnew Compositions and methods for detection and isolation of phosphorylated molecules
US20060127965A1 (en) * 2002-12-31 2006-06-15 Charlie Rodi Bindingzyme arrays and high-throughput proteomic methods
CA2514363A1 (en) * 2003-02-12 2004-08-26 Transtech Pharma, Inc. Substituted azole derivatives as therapeutic agents
US20040186151A1 (en) * 2003-02-12 2004-09-23 Mjalli Adnan M.M. Substituted azole derivatives as therapeutic agents
EP1730118A1 (en) * 2004-02-12 2006-12-13 Transtech Pharma, Inc. Substituted azole derivatives, compositions, and methods of use
AU2007211319B9 (en) 2006-01-30 2012-05-31 Vtv Therapeutics Llc Substituted imidazole derivatives and their use as PTPase inhibitors
US20080171351A1 (en) * 2006-05-22 2008-07-17 University Of Notre Dame Du Lac Probes for anionic cell surface detection
US20130078660A1 (en) * 2010-03-23 2013-03-28 Salk Institute For Biological Studies Methods and compositions for detecting protein modifications
JP5854545B1 (en) * 2015-04-30 2016-02-09 大学共同利用機関法人自然科学研究機構 Screening method for inhibitors of receptor protein tyrosine phosphatases belonging to R3RPTP subfamily
CN113899902B (en) * 2020-06-22 2024-10-29 上海科技大学 A method for identifying tyrosine phosphatase substrates
CN111812183B (en) * 2020-07-27 2022-05-17 山东理工大学 Preparation method and application of intramolecular photo-electrochemical sensor
CN116183565A (en) * 2022-12-09 2023-05-30 青岛大学 Method for Rapidly Detecting the Binding Ability of Small Molecule Compounds to FXR Receptor
CN117736257B (en) * 2023-12-18 2024-07-19 嘉华药锐科技(北京)有限公司 Tyrosine phosphorylation polypeptide enrichment method and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE920151A1 (en) * 1991-01-18 1992-07-29 Univ New York A novel expression-cloning method for identifying target¹proteins for receptor tyrosine kinases and novel human grb¹proteins
US5804395A (en) * 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
US5912138A (en) * 1996-07-25 1999-06-15 Cold Spring Harbor Laboratory Substrate trapping protein tyrosine phosphatases
ATE233325T1 (en) * 1997-12-05 2003-03-15 Upjohn Co FLUORESCENCE-BASED HTS (HIGH THROUGHPUT SCREENING) ASSAY METHODS FOR PROTEIN KINASE AND PHOSPHATASE

Also Published As

Publication number Publication date
US20020009762A1 (en) 2002-01-24
CA2435507A1 (en) 2001-08-23
WO2001061031A2 (en) 2001-08-23
US20070015231A1 (en) 2007-01-18
WO2001061031A9 (en) 2002-10-17
WO2001061031A3 (en) 2002-03-07
EP1257824A2 (en) 2002-11-20

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