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AU2001234883A1 - Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion - Google Patents

Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion

Info

Publication number
AU2001234883A1
AU2001234883A1 AU2001234883A AU3488301A AU2001234883A1 AU 2001234883 A1 AU2001234883 A1 AU 2001234883A1 AU 2001234883 A AU2001234883 A AU 2001234883A AU 3488301 A AU3488301 A AU 3488301A AU 2001234883 A1 AU2001234883 A1 AU 2001234883A1
Authority
AU
Australia
Prior art keywords
single nucleotide
restriction endonuclease
nucleotide polymorphisms
melting curve
endonuclease digestion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001234883A
Inventor
Joshua M. Akey
Mark D. Shriver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Penn State Research Foundation
Original Assignee
Penn State Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Research Foundation filed Critical Penn State Research Foundation
Publication of AU2001234883A1 publication Critical patent/AU2001234883A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001234883A 2000-02-10 2001-02-07 Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion Abandoned AU2001234883A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18161800P 2000-02-10 2000-02-10
US60181618 2000-02-10
US19685500P 2000-04-12 2000-04-12
US60196855 2000-04-12
PCT/US2001/003893 WO2001059144A1 (en) 2000-02-10 2001-02-07 Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion

Publications (1)

Publication Number Publication Date
AU2001234883A1 true AU2001234883A1 (en) 2001-08-20

Family

ID=26877345

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001234883A Abandoned AU2001234883A1 (en) 2000-02-10 2001-02-07 Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion

Country Status (3)

Country Link
US (1) US6506568B2 (en)
AU (1) AU2001234883A1 (en)
WO (1) WO2001059144A1 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6979541B1 (en) * 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
CA2483489C (en) * 2002-04-26 2015-12-08 University Of Utah Research Foundation Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye
AU2003234938A1 (en) * 2002-05-21 2003-12-02 Adgene Co., Ltd. Method of identifying nucleic acid
US20060094010A1 (en) * 2002-06-28 2006-05-04 Giles Robert C Methods and compositions for analyzing comprised sample using single nucleotide polymorphism panels
KR100477766B1 (en) * 2002-09-26 2005-03-21 (주)진매트릭스 Primers and method for detecting base mutation
AU2003286573B2 (en) * 2002-10-23 2009-09-10 Idaho Technology, Inc. Amplicon melting analysis with saturation dyes
AU2002338167A1 (en) * 2002-10-24 2004-05-13 Ben Gao A 4-dimensional method and equipment of detecting nucleic acid hybrid pair on the gene chip
AU2004265581B2 (en) * 2003-05-20 2008-06-05 Investigen, Inc System for detecting polynucleotides
DE10324912A1 (en) * 2003-05-30 2005-01-05 Siemens Ag Method for the detection of DNA point mutations (SNP analysis) and associated arrangement
US7381818B2 (en) * 2003-10-28 2008-06-03 Epoch Biosciences, Inc. Fluorescent probes containing 5′-minor groove binder, fluorophore and quenching moieties and methods of use thereof
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US20050255483A1 (en) * 2004-05-14 2005-11-17 Stratagene California System and method for smoothing melting curve data
KR100808312B1 (en) * 2004-07-26 2008-02-27 홍경만 Method for measuring the chromosome, gene or nucleotide sequence copy numbers using co-amplification of artificial SNP sequences
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
GB0512116D0 (en) * 2005-06-14 2005-07-20 Matforsk Norwegian Food Res In Classification method
US20070198653A1 (en) * 2005-12-30 2007-08-23 Kurt Jarnagin Systems and methods for remote computer-based analysis of user-provided chemogenomic data
US8021839B2 (en) * 2006-02-24 2011-09-20 Investigen, Inc. Methods and compositions for detecting polynucleotides
US7771947B2 (en) * 2007-02-23 2010-08-10 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
ES2582602T3 (en) 2007-03-28 2016-09-14 Signal Diagnostics High resolution nucleic acid analysis system and method to detect sequence variations
CN101663408B (en) * 2007-12-26 2012-11-28 爱科来株式会社 Melting curve analyzing method and melting curve analyzing device
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
JP6059453B2 (en) * 2011-05-31 2017-01-11 アークレイ株式会社 A method for detecting a plurality of gene polymorphisms at a single wavelength using a plurality of oligonucleotides modified with a fluorescent dye having the same or near detection wavelength
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP2880184B1 (en) * 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
US20160230231A1 (en) * 2013-10-18 2016-08-11 Institut De Cardiologie De Montreal Genotyping tests and methods for evaluating plasma creatine kinase levels
CN103589797A (en) * 2013-11-12 2014-02-19 中国农业科学院蔬菜花卉研究所 SNP (single nucleotide polymorphism) genotyping method and application thereof
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110709A (en) * 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
AUPO882497A0 (en) 1997-08-29 1997-09-18 Grains Research & Development Corporation A method of genotyping
GB9821989D0 (en) 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
GB0020633D0 (en) 2000-08-22 2000-10-11 Moulton Development Ltd Recumbent bicycle

Also Published As

Publication number Publication date
US20020098484A1 (en) 2002-07-25
WO2001059144A1 (en) 2001-08-16
US6506568B2 (en) 2003-01-14

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