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AU2001234775A1 - Direct detection and mutation analysis of low copy number nucleic acids - Google Patents

Direct detection and mutation analysis of low copy number nucleic acids

Info

Publication number
AU2001234775A1
AU2001234775A1 AU2001234775A AU3477501A AU2001234775A1 AU 2001234775 A1 AU2001234775 A1 AU 2001234775A1 AU 2001234775 A AU2001234775 A AU 2001234775A AU 3477501 A AU3477501 A AU 3477501A AU 2001234775 A1 AU2001234775 A1 AU 2001234775A1
Authority
AU
Australia
Prior art keywords
nucleic acids
copy number
direct detection
low copy
mutation analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001234775A
Inventor
Jill M. Kolesar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of AU2001234775A1 publication Critical patent/AU2001234775A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2001234775A 2000-02-04 2001-02-02 Direct detection and mutation analysis of low copy number nucleic acids Abandoned AU2001234775A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/499,003 US6261781B1 (en) 1997-08-05 2000-02-04 Direct detection and mutation analysis of low copy number nucleic acids
US09499003 2000-02-04
PCT/US2001/003482 WO2001057258A2 (en) 2000-02-04 2001-02-02 Direct detection and mutation analysis of low copy number nucleic acids

Publications (1)

Publication Number Publication Date
AU2001234775A1 true AU2001234775A1 (en) 2001-08-14

Family

ID=23983392

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001234775A Abandoned AU2001234775A1 (en) 2000-02-04 2001-02-02 Direct detection and mutation analysis of low copy number nucleic acids

Country Status (3)

Country Link
US (1) US6261781B1 (en)
AU (1) AU2001234775A1 (en)
WO (1) WO2001057258A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7083920B2 (en) * 2001-05-18 2006-08-01 Nagaoka & Co. Ltd. Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto
DE10163677A1 (en) * 2001-12-21 2003-07-10 Henkel Kgaa Method for the quantitative determination of gene expression
US20050019901A1 (en) * 2002-01-31 2005-01-27 Evgenia Matveeva Methods for synthesis of bio-active nanoparticles and nanocapsules for use in optical bio-disc assays and disc assembly including same
US20050118603A1 (en) * 2002-10-11 2005-06-02 Ahram Biosystems Inc. Target detection system having a conformationally sensitive probe comprising a nucleic acid based signal transducer
EP1687442B1 (en) * 2003-11-17 2008-04-09 Agilent Technologies, Inc. Nucleic acid detection
WO2005098031A1 (en) * 2004-04-06 2005-10-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Liquid-based hybridization assay
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
AU2013236175A1 (en) * 2012-03-22 2014-10-16 Kanagawa Prefectural Hospital Organization Method for identification and detection of mutant gene using intercalator
CN105181778B (en) * 2015-09-06 2017-12-05 常州大学 A kind of method of Capillary Electrophoresis quick detection base number of mismatches
US20240410857A1 (en) * 2021-01-27 2024-12-12 Beth Israel Deaconess Medical Center Electrophoretic mobility shift as a molecular beacon-based readout for mirna detection

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8902689D0 (en) 1989-02-07 1989-03-30 Ici Plc Assay method
JP2833063B2 (en) 1989-10-31 1998-12-09 株式会社島津製作所 Nucleic acid detection method
WO1993013223A1 (en) 1991-12-23 1993-07-08 Chiron Corporation Hiv probes for use in solution phase sandwich hybridization assays
JP3189000B2 (en) 1994-12-01 2001-07-16 東ソー株式会社 Specific nucleic acid sequence detection method
IT1282607B1 (en) * 1996-02-13 1998-03-31 Pier Giorgio Righetti USE OF MULTIPLE GRADIENTS FOR THE ANALYSIS OF MUTATIONS IN HUMAN GENOMIC DNA
US6013442A (en) * 1997-08-05 2000-01-11 Wisconsin Alumni Res Found Direct quantitation of low copy number RNA

Also Published As

Publication number Publication date
US6261781B1 (en) 2001-07-17
WO2001057258A2 (en) 2001-08-09
WO2001057258A3 (en) 2002-04-18

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