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AU2001226395A1 - Techniques for growth and differentiation of human pluripotent stem cells - Google Patents

Techniques for growth and differentiation of human pluripotent stem cells

Info

Publication number
AU2001226395A1
AU2001226395A1 AU2001226395A AU2639501A AU2001226395A1 AU 2001226395 A1 AU2001226395 A1 AU 2001226395A1 AU 2001226395 A AU2001226395 A AU 2001226395A AU 2639501 A AU2639501 A AU 2639501A AU 2001226395 A1 AU2001226395 A1 AU 2001226395A1
Authority
AU
Australia
Prior art keywords
differentiation
growth
techniques
stem cells
pluripotent stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001226395A
Inventor
Melissa K Carpenter
Walter D. Funk
Joseph D. Gold
Margaret S Inokuma
Chunhui Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Geron Corp
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corp filed Critical Geron Corp
Publication of AU2001226395A1 publication Critical patent/AU2001226395A1/en
Abandoned legal-status Critical Current

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
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    • C12N2500/62—DMSO
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/39—Steroid hormones
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    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
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    • C12N2503/00—Use of cells in diagnostics
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2001226395A 2000-01-11 2001-01-10 Techniques for growth and differentiation of human pluripotent stem cells Abandoned AU2001226395A1 (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US17558100P 2000-01-11 2000-01-11
US60175581 2000-01-11
US21374000P 2000-06-22 2000-06-22
US21373900P 2000-06-22 2000-06-22
US60213740 2000-06-22
US60213739 2000-06-22
US21638700P 2000-07-07 2000-07-07
US60216387 2000-07-07
US22006400P 2000-07-21 2000-07-21
US60220064 2000-07-21
US09688031 2000-10-10
US09/688,031 US6667176B1 (en) 2000-01-11 2000-10-10 cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
PCT/US2001/001030 WO2001051616A2 (en) 2000-01-11 2001-01-10 Techniques for growth and differentiation of human pluripotent stem cells

Publications (1)

Publication Number Publication Date
AU2001226395A1 true AU2001226395A1 (en) 2001-07-24

Family

ID=42331357

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2001226395A Abandoned AU2001226395A1 (en) 2000-01-11 2001-01-10 Techniques for growth and differentiation of human pluripotent stem cells
AU11128/01A Expired AU751321B2 (en) 2000-01-11 2001-01-11 Techniques for growth and differentiation of human pluripotent stem cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU11128/01A Expired AU751321B2 (en) 2000-01-11 2001-01-11 Techniques for growth and differentiation of human pluripotent stem cells

Country Status (8)

Country Link
US (13) US6667176B1 (en)
EP (2) EP1250420A2 (en)
JP (2) JP2003530828A (en)
CN (2) CN1416462A (en)
AU (2) AU2001226395A1 (en)
CA (1) CA2388811C (en)
IL (2) IL141742A (en)
WO (1) WO2001051616A2 (en)

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