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AU2001294734A1 - Calcium free subtilisin mutants - Google Patents

Calcium free subtilisin mutants

Info

Publication number
AU2001294734A1
AU2001294734A1 AU2001294734A AU9473401A AU2001294734A1 AU 2001294734 A1 AU2001294734 A1 AU 2001294734A1 AU 2001294734 A AU2001294734 A AU 2001294734A AU 9473401 A AU9473401 A AU 9473401A AU 2001294734 A1 AU2001294734 A1 AU 2001294734A1
Authority
AU
Australia
Prior art keywords
calcium free
subtilisin mutants
free subtilisin
mutants
calcium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001294734A
Inventor
Philip N Bryan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
The University of Maryland Biotechnology Institute
Original Assignee
The University of Maryland Biotechnology Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University of Maryland Biotechnology Institute filed Critical The University of Maryland Biotechnology Institute
Publication of AU2001294734A1 publication Critical patent/AU2001294734A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2001294734A 2000-09-29 2001-09-26 Calcium free subtilisin mutants Abandoned AU2001294734A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/672,105 US6541235B1 (en) 2000-09-29 2000-09-29 Calcium free subtilisin mutants
US09/672,105 2000-09-29
PCT/US2001/030092 WO2002026956A1 (en) 2000-09-29 2001-09-26 Calcium free subtilisin mutants

Publications (1)

Publication Number Publication Date
AU2001294734A1 true AU2001294734A1 (en) 2002-04-08

Family

ID=24697163

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001294734A Abandoned AU2001294734A1 (en) 2000-09-29 2001-09-26 Calcium free subtilisin mutants

Country Status (3)

Country Link
US (1) US6541235B1 (en)
AU (1) AU2001294734A1 (en)
WO (1) WO2002026956A1 (en)

Families Citing this family (18)

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US7172892B2 (en) * 2001-03-22 2007-02-06 Dendreon Corporation Nucleic acid molecules encoding serine protease CVSP14, the encoded polypeptides and methods based thereon
US7112430B2 (en) * 2001-05-14 2006-09-26 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
WO2003044179A2 (en) * 2001-11-20 2003-05-30 Dendreon San Diego Llc Nucleic acid molecules encoding serine protease 17, the encoded polypeptides and methods based thereon
BRPI0306955B1 (en) * 2002-01-16 2016-03-22 Danisco Us Inc bacillus lentus subtilisin protease variant, dna, expression vector, prokaryotic host cell, as well as cleaning composition
WO2004005471A2 (en) * 2002-07-02 2004-01-15 Dendreon Corporation Serine protease 16
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
EP1651751B1 (en) * 2003-08-06 2015-11-18 University Of Maryland Biotechnology Institute Engineered proteases for affinity purification and processing of fusion proteins
US7642080B2 (en) 2005-08-16 2010-01-05 Novozymes A/S Subtilases
US20080090747A1 (en) * 2006-07-18 2008-04-17 Pieter Augustinus Protease variants active over a broad temperature range
MX350188B (en) * 2008-06-06 2017-08-30 Danisco Us Inc Compositions and methods comprising variant microbial proteases.
US10138268B2 (en) * 2014-10-10 2018-11-27 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
US10752931B2 (en) 2015-07-09 2020-08-25 Enzypep B.V. Designing an enzymatic peptide fragment condensation strategy
CN104973738B (en) * 2015-07-26 2017-01-18 衢州学院 A method for removing chromium-containing sewage pollutants
EP3404036A1 (en) * 2017-05-19 2018-11-21 Enzypep B.V. Subtilisin variants having a mutation in the s2 or s2' pocket
EP3679133B1 (en) * 2017-09-05 2024-11-06 Henkel AG & Co. KGaA Performance-enhanced protease variants i
WO2019170895A1 (en) * 2018-03-09 2019-09-12 Enzypep B.V. Chemo-enzymatic synthesis of semaglutide, liraglutide and glp-1
US10883132B2 (en) 2018-03-09 2021-01-05 Enzypep B.V. Chemo-enzymatic synthesis of Semaglutide, Liraglutide and GLP-1
US12497644B2 (en) 2021-02-12 2025-12-16 Fresenius Kabi Ipsum S.R.L. Subtilisin variants and their use

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US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5700676A (en) * 1984-05-29 1997-12-23 Genencor International Inc. Modified subtilisins having amino acid alterations
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5371008A (en) * 1984-05-29 1994-12-06 Genencor International, Inc. Substrate assisted catalysis
US5371190A (en) * 1984-05-29 1994-12-06 Genencor International, Inc. Substrate assisted catalysis
US5346823A (en) * 1984-05-29 1994-09-13 Genencor, Inc. Subtilisin modifications to enhance oxidative stability
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (en) * 1988-01-07 1988-01-07 Novo Industri As ENZYMES
PT89702B (en) * 1988-02-11 1994-04-29 Gist Brocades Nv PROCESS FOR PREPARING NEW PROTEOLITIC ENZYMES AND DETERGENTS THAT CONTAINS THEM
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
JP2739982B2 (en) * 1988-04-11 1998-04-15 株式会社リコー Toner for developing electrostatic images
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5246849A (en) * 1988-04-12 1993-09-21 Enzon, Inc. Thermally stable serine proteases
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
WO1992002615A1 (en) * 1990-08-09 1992-02-20 Genentech, Inc. Serine protease variants having peptide ligase activity
DK271490D0 (en) * 1990-11-14 1990-11-14 Novo Nordisk As detergent composition
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
EP0525610A3 (en) * 1991-07-27 1993-03-24 Solvay Enzymes Gmbh & Co. Kg Process for increasing the stability of enzymes and stabilized enzymes
US5275945A (en) * 1991-10-08 1994-01-04 Vista Chemical Company Alkaline proteases stable in heavy-duty detergent liquids
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
JP2888985B2 (en) * 1993-10-14 1999-05-10 ザ、プロクター、エンド、ギャンブル、カンパニー Protease-containing cleaning composition
EP0723580B1 (en) * 1993-10-14 2003-07-16 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
ES2364776T3 (en) * 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA IMPROVED AND DETERGENT ENZYMES THAT CONTAIN THEM.
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues

Also Published As

Publication number Publication date
WO2002026956A1 (en) 2002-04-04
US6541235B1 (en) 2003-04-01
WO2002026956A9 (en) 2003-05-01

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